CN117356571A

CN117356571A - Seed treatment agent containing allicin

Info

Publication number: CN117356571A
Application number: CN202311306940.9A
Authority: CN
Inventors: 李真真; 陈庭倬; 李艳芬; 龚宝金; 周海伟; 彭涛; 何洁; 牟天秀; 殷浩; 任丹; 何其明
Original assignee: Chengdu New Chaoyang Crop Science Co ltd
Current assignee: Chengdu New Chaoyang Crop Science Co ltd
Priority date: 2022-10-19
Filing date: 2023-10-10
Publication date: 2024-01-09

Abstract

The invention discloses a seed treating agent containing allicin, which contains 0.5-20% of allicin. The seed treated by the seed treating agent has the functions of preventing and controlling plant fungi, improving the germination rate of the seed, and increasing the plant height, root length and fresh weight of the plant.

Description

Seed treatment agent containing allicin

Technical Field

The invention belongs to the technical field of agricultural breeding, and particularly relates to a seed treatment agent containing allicin.

Background

Chemical pesticides are a common means for preventing and controlling crop diseases at present, but a large amount of chemical pesticides cause residues of harmful substances in plant bodies, seriously pollute crops, lands, water sources and the like, and endanger human and animal safety. Therefore, there is an urgent need to search for and develop new pesticides that are friendly to human health and ecology. The botanical pesticide is natural, can be degraded in nature, generally does not pollute the environment and agricultural products, has little possibility of accumulating toxicity in the environment and human bodies, is relatively safe to people and livestock, has little harm to natural enemies of pests, and is difficult to generate antibodies to the pests, so that the botanical pesticide is one of the solutions.

The botanical pesticide is also called as botanical pesticide, and the pesticide developed by utilizing plant resources is an important component of biological pesticide. The plant source preparation is prepared by using stable effective components contained in certain parts (roots, stems, leaves, flowers or fruits) of plants, and using the plant source preparation to prevent or reduce the damage of pests such as diseases, insects, weeds and the like after the acceptor plants are used according to a certain method. Comprises active ingredients extracted from plants, the plants themselves and compounds and derivatives synthesized according to the active structures.

Allicin is a recognized plant source antibiotic, and the chemical structural formula of allicin is CH=CH-CH ₂ -S-SCH-CH＝CH ₂ O, the allicin molecule has a pair of active groups-S (0) -S-formed by coupled sulfur atoms, which are active groups important for the antimicrobial action of the allicin. In recent years, allicin has been widely studied for its application in livestock, poultry and aquatic animal breeding and for controlling plant diseases and insect pests. The allicin has a certain inhibition effect on certain pathogenic bacteria, has obvious control effect on bacteria, can be used for preventing and treating diseases of various crops such as oranges, kiwi fruits, melons, eggplants, leaf vegetables and the like caused by the bacterial pathogenic bacteria, such as angular leaf spot, soft rot, ulcer, bacterial wilt and the like, has the advantages of quick degradation, low toxicity, difficult generation of drug resistance, difficult generation of harmful substances, environmental friendliness and the like compared with chemical pesticides, has the characteristics of no toxic or side effect, no drug residue, no drug resistance and the like, and becomes a good choice for replacing antibiotics to produce safe and pollution-free pesticides, but has short duration, and has the defect of application such as the allicin being extremely easy to decompose under high temperature and alkaline conditions. The allicin content can not be detected after the mass spectrum method detects that the allicin microemulsion is irrigated into the soil for 7 days, the existing allicin dosage form has short duration and severely restricts the application and popularization.

Disclosure of Invention

In order to solve the problems in the prior art, the present invention provides a seed treatment agent containing allicin.

The seed treatment is a crop protection means which is very popular in the world today, and mainly utilizes a seed treatment agent to treat crop seeds so as to achieve the effects of preventing diseases and insect pests, improving the sprouting quality of the seeds, promoting the root system development, improving the stress resistance of seedlings and the like, thereby leading crops to grow better. In recent years, the market share of seed treatments for various crops worldwide including corn, soybean, cotton, rice, canola and the like has continued to increase. Although the plant seed treatment agents in the current market have a plurality of types, the defects of poor sterilization and disease resistance and poor germination accelerating effect generally exist, and the yield and the growth of plant seeds are limited. If the allicin can be prepared into a plant seed treating agent, the allicin can effectively sterilize and improve the germination rate of seeds and promote the growth of seedlings.

In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:

a seed treatment agent containing allicin, which contains 0.5-20% allicin, further 1-10%; for example, the garlicin may be selected from 0.5%, 1%, 2%, 3%, 5%, 7%, 8%, 10%, 12%, 15%, 16%, 17%, 19% or 20% of the total content.

Further, the seed treatment agent contains a solvent in an amount of 0.5 to 15%, and is further selected from 1 to 10%; for example, the solvent may be selected from 0.5%, 1%, 2%, 3%, 5%, 7%, 8%, 9%, 10%, 11%, 13%, 15% or the like.

Further, the seed treatment agent contains an emulsifier in an amount of 0.5 to 20%, and is further selected from 1 to 10%; for example, the emulsifier may be selected from the group consisting of 0.5%, 1%, 2%, 3%, 5%, 7%, 8%, 10%, 12%, 15%, 16%, 17%, 19% and 20% of the total content.

Further, the seed treatment agent contains an antioxidant in an amount of 0.05 to 1%, and is further selected from 0.05 to 0.5%; for example, the antioxidant may be selected from 0.05%, 0.06%, 0.08%, 0.1%, 0.2%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1% of the antioxidant.

Further, the seed treatment agent contains a film forming agent, the content of which is 0.5-15%, and is further selected from 1-10%; for example, 0.5%, 1%, 2%, 3%, 5%, 7%, 8%, 9%, 10%, 11%, 13%, 15% or the like may be selected.

Further, the seed treatment agent contains a dispersant in an amount of 0.5 to 20%, and is further selected from 1 to 10%; for example, 0.5%, 1%, 2%, 3%, 5%, 7%, 8%, 10%, 12%, 15%, 16%, 17%, 19% or 20% may be selected.

Further, the seed treatment agent contains microcapsule materials, the content is 0.5-20%, and is further selected from 1-10%; for example, 0.5%, 1%, 2%, 3%, 5%, 7%, 8%, 10%, 12%, 15%, 16%, 17%, 19% or 20% may be selected.

Further, the seed treatment agent contains warning color, the content is 0.5-15%, and is further selected from 1-10%; for example, 0.5%, 1%, 2%, 3%, 5%, 7%, 8%, 9%, 10%, 11%, 13%, 15% or the like may be selected.

Further, the seed treatment agent contains an antifreeze agent, the content of which is 0.5-20%, and is further selected from 1-10%; for example, 0.5%, 1%, 2%, 3%, 5%, 7%, 8%, 10%, 12%, 15%, 16%, 17%, 19% or 20% may be selected.

Further, the seed treatment agent contains a stabilizer in an amount of 0.01 to 0.1%, and is further selected from 0.01 to 0.05%; for example, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, … …, 0.09, or 0.1% may be selected.

Further, the seed treatment may contain other adjuvants including, but not limited to, liquid carriers, solid carriers, surfactants (surfactants), viscosity modifiers, rheology additives, structuring agents, preservatives, biocides or biological inhibitors, crystallization inhibitors, suspending agents, dyes, foaming agents, light absorbers, mixing adjuvants, defoamers, complexing agents, neutralizing or pH adjusting substances and buffering agents, corrosion inhibitors, fragrances, wetting agents, absorption promoters, micronutrients, plasticizers, glidants, lubricants, and the like.

As the existing allicin preparation is in the conventional dosage forms such as microemulsion and emulsifiable concentrate, the property of easy volatilization of the allicin under high temperature and alkaline conditions is not changed, meanwhile, the medicament is washed in rainy days during field application, the actual content of the allicin is reduced to obviously reduce the field prevention effect, the effective components are generally not easy to diffuse to the periphery rapidly after the seed treatment agent coats seeds, the effect of sun, rain and high temperature is avoided, the medicament is released slowly after sowing, the longer-term effect is maintained, the stability is improved, and the field application prevention effect is obviously improved.

In a specific embodiment of the present invention, the present invention provides a seed treatment agent containing allicin, which comprises 0.5 to 20% of allicin, 0.5 to 15% of a solvent, 0.5 to 20% of an emulsifier, 0.05 to 1% of an antioxidant, 0.5 to 15% of a film forming agent, 0.5 to 20% of a dispersing agent, 0.5 to 20% of a microcapsule material, 0.5 to 15% of a warning color, 0.5 to 20% of an antifreeze agent, and 0.01 to 0.1% of a stabilizer.

Further, 1 to 10 percent of allicin, 1 to 10 percent of solvent, 1 to 10 percent of emulsifier, 0.05 to 0.5 percent of antioxidant, 1 to 10 percent of film forming agent, 1 to 10 percent of dispersing agent, 1 to 10 percent of microcapsule material, 1 to 10 percent of warning color, 1 to 10 percent of antifreeze agent and 0.01 to 0.05 percent of stabilizer.

Further, the anti-freezing agent comprises 2 to 7 percent of allicin, 1 to 5 percent of solvent, 2 to 7 percent of emulsifier, 0.1 to 0.4 percent of antioxidant, 2 to 7 percent of film forming agent, 2 to 7 percent of dispersing agent, 2 to 7 percent of microcapsule material, 1 to 5 percent of warning color, 2 to 7 percent of antifreeze agent and 0.01 to 0.05 percent of stabilizing agent.

In the present invention, the solvent includes, but is not limited to, an alkane solvent including at least one of the following components: solvent oil, benzene, toluene, xylene, trimethylbenzene, mixed benzene; the solvent oil is 150# solvent oil.

In the invention, the emulsifier at least comprises one of the following components: castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, phenethyl phenol polyoxyethylene ether and calcium dodecyl benzene sulfonate.

In the invention, the antioxidant at least comprises one of the following components: methyl esterified soybean oil, rosemary essential oil, epigallocatechin gallate, phytic acid, propyl gallate, BHT, tea polyphenols, ascorbic acid, BHA, epoxidized soybean oil, and D-alpha tocopherol.

In some embodiments of the invention, the antioxidant comprises D-alpha tocopherol.

In the invention, the film forming agent at least comprises one of the following components: polyvinyl alcohol, polyvinyl acetate, carboxymethyl cellulose, gum arabic, gelatin, xanthan gum, starch, microcrystalline cellulose, polyethylene glycol, polystyrene-acrylic emulsion, polyvinylpyrrolidone, polyvinyl acetate.

In the invention, the dispersing agent at least comprises one of the following components: phosphate dispersants, sodium methyl naphthalene sulfonate formaldehyde condensate, alkylaryl sulfonate, alpha-alkenyl sulfonate, sodium dodecyl sulfate, succinate sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, alkylphenol ethoxylates, polystyrylphenol ethoxylates, castor oil ethoxylates, sorbitan fatty acid esters, sorbitan fatty acid ester ethoxylates, ethylene oxide propylene oxide block copolymers, phenolic resin polyoxyalkylene ethers, fatty alcohol polyoxyethylene ethers, fatty acid polyoxyalkylene ether esters, fatty amine polyoxyalkylene ethers.

In the invention, the microcapsule material at least comprises one of the following components: capsule wall material, polyurea, polyamide, polyurethane, polyester, urea-formaldehyde resin, polystyrene, polyurethane, melamine resin.

In the invention, the warning color at least comprises one of the following components: acid scarlet, rose bengal, rose essence, carmine, congo red.

In the invention, the antifreeze agent at least comprises one of the following components: ethylene glycol, glycerol, urea, propylene glycol, polyethylene glycol, and sorbitol.

In the invention, the stabilizer at least comprises one of the following components: diutan, guar gum, xanthan gum, sodium carboxymethyl cellulose, sodium polyacrylate, diatomite, bentonite, attapulgite, light calcium carbonate, white carbon black and clay.

In the present invention, the seed treatment formulation is beneficial for plant growth and/or protects plants from plant pests; the plant pest is at least one selected from plant fungal pathogens, plant bacterial pathogens, nematodes and insects.

The planting treatment preparation of the present invention may be formulated with a carrier and other auxiliary agents as needed, and is used as a preparation form usually used as a seed treatment agent, for example, as a solid preparation such as a powder, a hydration agent, or a particulate hydration agent, or as a liquid preparation such as a liquid preparation, an emulsion, a fluidity, or an emulsion preparation. In particular embodiments of the present invention, the plant treatment agent may be formulated in forms including, but not limited to, suspended seed coating agents, aqueous emulsions, microencapsulated suspensions, dispersible powders, dry mixes, microemulsions; in a specific embodiment of the invention, the preparation form of the plant treatment agent is an aqueous emulsion or a microcapsule suspension.

The present invention provides the use of said seed treatment in plant growth and/or for protecting plants from plant pests; the plant pest is at least one selected from plant fungal pathogens, plant bacterial pathogens, nematodes and insects.

The present invention provides a method of benefiting plant growth and/or protecting a plant from a plant pest, the method comprising: the allicin-containing composition of the invention is used in an amount suitable for plant growth and/or protection of plants from plant pests, to plants, plant parts, plant seeds, plant roots, soil or growth medium surrounding plants or seeds of plants or soil or growth medium prior to planting plants or sowing seeds of plants.

In a specific embodiment of this aspect, the invention provides a method of benefiting plant growth and/or protecting a plant from a plant pest, the method comprising: the plant seeds are treated with the aforementioned seed treatment agent.

In the seed treatment formulations and methods disclosed herein, the benefits of plant growth and/or protection of plants from plant pests may be manifested by increased seed germination, increased seed vigor, improved seedling vigor, improved root development, improved plant growth, improved plant health, increased yield, improved appearance, improved resistance to plant pests, reduced pathogen infection, or a combination thereof.

The seed treatment formulations and methods disclosed herein benefit a wide range of plants, including, but not limited to, monocots, dicots, cereal, such as corn, sweet corn, popcorn, seed corn, silage corn, feed corn, rice, wheat, barley, sorghum, asparagus, berries, such as blueberries, blackberries, raspberries, radishes, cowberry fruits, cranberries, currants, elderberries, raisins, cranberries, -bar berries, brassica vegetables, such as broccoli, cabbage, cauliflower, brussels sprouts, collards, mustard sprouts, corm cabbage, melon vegetables, such as cucumber, cantaloupe, melon, pumpkin fruits, watermelons, pumpkin fruits, eggplants, bulbs, such as onion, garlic, green onion, citrus fruits, such as orange, grapefruit, lemon, orange, tangerines, fruit, vegetables, such as onion, ground, green cherry, okra, grape, spice plants, such as coles, leaf vegetables, such as 35, celery, green beans, green vegetables, such as green beans, 37, or vegetables, including green beans, kidney beans, shell beans, soybeans, dried kidney beans, chickpeas, lima beans, peas, chickpeas, split peas, lentils, oil crops such as rape, castor, coconut, cotton, flax, oil palm, olives, peanuts, rapeseed, safflower, sesame, sunflower, soybeans, pome such as apples, crabapple, pears, quince, summer hawthorn, roots, tubers and bulb vegetables such as carrot, potato, sweet potato, tapioca, beet, ginger, horseradish, radish, ginseng, turnip, stone fruits such as apricot, cherry, nectarine, peach, plum, strawberry, tree nuts such as almond, pistachio, pecan, walnut, hazelnut, chestnut, cashew, beech nut, ash walnut, macadamia nut, kiwi, banana, (blue) agave, grass, turf grass, ornamental plant, poinsettia, hard cuttings such as chestnut, oak, maple, sugarcane and beet. In one or more embodiments, the plant may include corn, soybean, potato, cotton, tomato, pepper, melon, sugarcane, peanut, or wheat; or soybean, cotton, wheat, corn or potato.

In a specific embodiment of the invention, the seed treatment formulation of the invention is beneficial to corn.

The invention has the following beneficial effects:

(1) The seed treating agent can prolong the lasting period of allicin, clings the seed treating agent to the seed, has concentrated efficacy and high utilization rate, can reduce the pesticide application amount and application times compared with the conventional spraying, powder spraying and soil treatment, does not need subsequent secondary pesticide application, reduces environmental pollution and field operation procedures, is convenient for mechanical sowing and even sowing after the seed treating agent is adopted to coat the seed, and is one of the most economical and effective methods in plant pest control.

(2) Because the allicin has the pungent taste, the conventional dosage forms are applied by leaf spraying or root irrigation, have larger pungent taste and longer duration, and the seed treatment agent is hidden in use, so that the release of the pungent taste of the allicin can be greatly reduced, the atmospheric pollution is obviously reduced, natural enemies are not injured, and the preparation is safer to the environment.

(3) The seed treatment agent can effectively prevent and treat fungi such as banded sclerotial blight which attack corns, and remarkably improve the lasting period and the prevention effect of allicin on various agricultural diseases; the conventional allicin common dosage form has no influence on the root length and plant height of plants, and the seed treatment agent provided by the invention can improve the seed germination rate, enhance the germination vigor, improve the plant height, fresh weight and root length of seedlings and provide a certain technical support for the industrialization of corn planting industry.

Drawings

FIG. 1 is a graph of 5% allicin seed treatment (microcapsule suspension);

FIG. 2 is a graph of 5% allicin seed treatment (aqueous emulsion);

FIG. 3 is a graph of 5% allicin 01;

FIG. 4 is a graph of 5% garlicin 02;

FIG. 5 is a graph of 5% allicin 03;

FIG. 6 is a graph of 5% allicin 04;

FIG. 7 is a graph of 5% allicin 05;

FIG. 8 is a graph of 5% garlicin 06;

FIG. 9 is a graph of 5% garlicin 07;

FIG. 10 is a graph of 5% allicin 08;

FIG. 11 is a graph of 5% allicin 09;

FIG. 12 is a graph of 5% garlicin 10;

FIG. 13 is a graph of 5% allicin 11;

FIG. 14 is a graph of 5% allicin 12;

FIG. 15 is a graph of 5% garlicin 13;

FIG. 16 is a graph of 5% allicin 14;

FIG. 17 is a graph of 5% garlicin 15;

fig. 18 is a graph of 5% allicin 16.

Detailed Description

The following description of the present invention will be made clearly and fully, and it is apparent that the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to fall within the scope of the invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

Example 1

The microcapsule suspending agent is prepared by the following steps of preparing 5% of allicin, 3% of solvent, 5% of emulsifier, 0.2% of antioxidant, 3% of film forming agent, 5% of dispersing agent, 5% of microcapsule material, 3% of warning color, 5% of antifreeze agent, 0.02% of stabilizer and 100% of deionized water.

The preparation process comprises the following steps:

(1) Adding 10 g of 50% allicin mother liquor into a beaker, then adding 3 g of 150# solvent oil and 0.2 g of BHT, stirring and dissolving uniformly, then adding castor oil polyoxyethylene ether and isocyanate, continuously stirring uniformly, then adding 48.75 g of deionized water, shearing for 2min at 2000r/min to be uniform by using a shearing and dispersing machine, and transferring into a three-neck flask;

(2) Adding 2 g of triethylamine and 10 g of deionized water into a beaker, stirring uniformly, adding into a burette, and slowly adding into a three-neck flask at a rotating speed of 500-600 r/min;

(3) After the dripping is finished, opening a water bath to heat to 60-70 ℃ and preserving heat for 2h;

(4) After the incubation was completed, the mixture was transferred to a beaker, and then 3 g of polyacrylate, 3 g of TEGO DIPERS 655, 3 g of rose essence, 5g of urea, 0.02 g of xanthan gum and an antifoaming agent were addedAF 1500.03 g, and stirring and dissolving uniformly.

Example 2

The water emulsion is prepared by adding 5% of allicin, 3% of solvent, 5% of emulsifier, 0.2% of antioxidant, 3% of film forming agent, 5% of dispersing agent, 3% of warning color, 5% of antifreeze agent, 0.02% of stabilizing agent and 100% of deionized water.

The preparation process comprises the following steps:

(1) Adding 10 g of 50% allicin mother liquor into a beaker, then adding 3 g of 150# solvent oil and 0.2 g of BHT, stirring and dissolving uniformly, then adding castor oil polyoxyethylene ether, continuously stirring uniformly, then adding 65.75 g of deionized water, and shearing for 2min to uniformity at 2000r/min by a shearing and dispersing machine;

(2) Adding 3 g of polyacrylate, 655 g of TEGO DIPERS 655, 3 g of rose essence, 5g of urea, 0.02 g of xanthan gum and an antifoaming agent into a beakerAF 1500.03 g, and stirring and dissolving uniformly.

Example 3

Different effects of allicin seed treatment agent (aqueous emulsion) on corn seed growth

Test agent: 5% allicin seed treatment agent (aqueous emulsion), aqueous emulsion solvent control

Test materials: corn (variety: xianyu 335)

Control agent: 6% allicin aqueous emulsion (allicin content 60g/L, amino acid content 100g/L, trace element content 100 g/L), the research shows that the medicament can obviously promote plant growth (refer to the research (Zhouqing, jiangxi agricultural university) of the bactericidal activity of 6% allicin aqueous emulsion and the influence thereof on crop growth, the research of the bactericidal activity of 6% allicin aqueous emulsion on the crop growth, the medicament has a seed ratio of 1:150 is the optimal growth promoting concentration in the paper, which is used as a control agent to demonstrate the superiority of the agents of the invention.

Fludioxonil (active ingredient 25 g/L), the most common seed coating agent in the market at present, therefore, fludioxonil is selected as a control for experiments. The manufacturing factory: first, n-das crop protection limited, pesticide registration number: PD20150099, pesticide production license number: pesticide Shengsheng (su) 0041, product standard number: Q/320601ZN 018-2019, date of manufacture: 20210127.

test materials: tray, filter paper, balance, gun head, disposable self-sealing bag, spray can, pipette (dragon-lab 100-1000 μl), slide caliper, SPAD tester (chlorophyll tester).

And (3) test design:

the test contains 2 medicament treatments, and the medicament seed ratios are respectively 1:100, 1:500 and 1: 1000. 1: 10000. 1:100000, 13 treatments (with blank clear water control and chemical control), each treatment was repeated 3 times, 18 seeds were used for each treatment, and each test treatment was consistent with other management measures and fertilizer water conditions except for the use of different agents.

Firstly, preparing the medicament according to the seed dressing ratio of table 1 for later use, weighing corn seeds, placing the corn seeds in a sealing bag (10 cm multiplied by 15 cm), adding a certain amount of the prepared medicament, sealing, and repeatedly shaking until each seed is uniformly adhered with the test medicament. And then spreading the seeds wrapped with the medicament for 24 hours, airing, sealing after airing, and storing at normal temperature. And (2) selecting normal and full seeds on the 2 nd day, uniformly placing the seeds in a prepared seedling raising tray, watering the seedling raising tray thoroughly in advance, placing the seedling raising tray in a 25 ℃ environment for culturing, and investigating after 7 days.

The plant height, root length, plant fresh weight, root fresh weight, germination rate, leaf width, leaf thickness, SPAD and stem thickness of corn were measured, respectively, and data analysis was performed.

The test results are shown in tables 1 to 9:

TABLE 1 influence of different treatments on plant height

TABLE 2 Effect of different treatments on root length

TABLE 3 influence of different treatments on plant fresh weight

TABLE 4 Effect of different treatments on root fresh weight

TABLE 5 influence of different treatments on germination rate

TABLE 6 influence of different treatments on leaf width

TABLE 7 effects of different treatments on leaf thickness

TABLE 8 influence of different treatments on stem thickness

TABLE 9 Effect of different treatments on SPAD values

The results in tables 1 to 9 show that there is no difference between germination rate and SPAD value for different treatments, while the ratio of allicin seed treatment agent (aqueous emulsion) to seed treatment agent of 5% is 1:500, the plant height, root length, plant fresh weight, root fresh weight, leaf width, leaf thickness and stem thickness are increased most obviously, the increasing amount is gradually reduced along with the improvement of the medicine seed ratio, and the medicine seed ratio is 1: at 500 hours, various indexes are obviously better than the optimal concentration of the 6% allicin aqueous emulsion.

Example 4

Different effects of allicin seed treatment (microcapsule suspension) on corn seed growth

Test agent: 5% allicin seed treatment agent (microcapsule suspending agent of the invention), microcapsule suspending agent solvent pair

Illumination device

Test materials, control agents, test materials, test designs are all referred to in example 3.

The test results are shown in tables 10 to 18:

TABLE 10 influence of different treatments on plant height

TABLE 11 Effect of different treatments on root Length

TABLE 12 influence of different treatments on plant fresh weight

TABLE 13 Effect of different treatments on root fresh weight

TABLE 14 Effect of different treatments on germination Rate

TABLE 15 influence of different treatments on leaf width

TABLE 16 effects of different treatments on leaf thickness

TABLE 17 effects of different treatments on stem thickness

TABLE 18 effects of different treatments on SPAD values

The results in tables 10 to 18 show that there is no difference between germination rate and SPAD value for the different treatments, while the ratio of 5% allicin seed treatment (microcapsule suspension) is 1: the plant height, root length, plant fresh weight, root fresh weight, leaf width, leaf thickness and stem thickness increase are most remarkable when 1000, the increment is gradually reduced along with the improvement of the medicine seed ratio, and the medicine seed ratio is 1: the various indexes are obviously better than the optimal concentration of the 6% allicin aqueous emulsion at 1000.

Example 5

Allicin seed treatment agent (aqueous emulsion) has effect of preventing corn sheath blight

Test agent: 5% allicin seed treatment agent (aqueous emulsion of the invention), aqueous emulsion solvent control

Test materials: corn (variety: xianyu 335)

Control agent: 6% allicin aqueous emulsion (allicin content 60g/L, amino acid content 100g/L, trace element content 100 g/L), the research shows that the medicament can obviously inhibit pathogenic bacteria (refer to '6% allicin aqueous emulsion bactericidal activity and research on crop growth influence' paper), and the medicament has a specific ratio of 1:50 is the optimal inhibitory concentration in the paper, which is used as a control medicament to prove the superiority of the medicament.

test materials: tray, filter paper, balance, gun head, disposable self-sealing bag, spray can, pipetting gun (dragon-lab 100-1000 muL)

Control object: banded sclerotial blight

And (3) test design: the test contains 2 medicament treatments, the medicament seed ratio is 1:100, 1:500 and 1:1000 respectively, 9 treatments (including blank clear water control and chemical control) are added to seed corn, each treatment is repeated for 3 times, 20 seeds are used for each treatment, and other management measures and fertilizer and water conditions are kept consistent except for different medicaments.

The preparation is prepared according to Table 19, corn seeds are weighed and placed in a sealing bag (10 cm multiplied by 15 cm), a certain amount of prepared preparation is added, and the mixture is repeatedly shaken after sealing until each seed is uniformly adhered with the test preparation. And then spreading the seeds wrapped with the medicament for 24 hours, airing, sealing after airing, and storing at normal temperature. On the 2 nd day, selecting normal and full seeds, uniformly placing the seeds in prepared nutrient soil, and pre-mixing the nutrient soil and the rhizoctonia solani culture solution according to the following formula 1:100 mass ratio, and culturing in 25 deg.c environment for 14 days for investigation.

Survey data and calculation: and calculating average number and preventing effect according to pesticide field efficacy test criterion.

The test results are shown in Table 19:

TABLE 19 control of maize sheath blight by different treatments

Example 6

Allicin seed treatment agent (microcapsule suspending agent) has effect of preventing corn sheath blight

Test agent: 5% allicin seed treatment (microcapsule suspension) and microcapsule suspension solvent control

Test material, control agent, test material, test design reference example 5.

The test results are shown in Table 20:

TABLE 20 control of maize sheath blight by different treatments

As can be seen from tables 19-20, for maize sheath blight, the 5% allicin seed treatment (aqueous emulsion) drug seed ratio was 1: the control effect on the sheath blight of corn at 100 reaches 73.76%, and the medicine seed ratio of the 5% allicin seed treatment agent (microcapsule suspending agent) is 1: the control effect on the sheath blight of corn reaches 74.03% at 100, and the control effect on the sheath blight of corn gradually decreases along with the increase of the seed dressing ratio, and the seed dressing ratio is 1: the prevention effect is obviously better than that of 6% allicin emulsion in water and fludioxonil at 100.

In general, the 5% allicin seed coating agent (aqueous emulsion and microcapsule suspending agent) has the effect obviously better than the 6% allicin aqueous emulsion which is studied in terms of plant growth promotion, including plant height, root length, plant fresh weight, root fresh weight, stem thickness and other indexes, and also has the effect obviously better than the fludioxonil which is a common chemical seed coating agent in terms of plant infection resistance to pathogenic bacteria. On the premise of improving the effect, the dosage is far lower than the optimal concentration of the allicin aqueous emulsion of 6%, and the use cost is greatly reduced. The garlicin seed coating agent can improve the application value of garlicin in fields in multiple aspects, and truly realizes multiple purposes.

Example 7

Effect of different antioxidants on allicin stability

The test method comprises the following steps: according to the property of garlicin, garlicin is stable at normal temperature and is easy to decompose at high temperature, the invention aims to develop a novel pesticide containing garlicin, guide agricultural production and carry out according to the GBT 19136-2003 pesticide heat storage stability measuring method. The allicin is prepared into a preparation with the content of 5 percent, and then the preparation is stored for 14 days at the temperature of 54 ℃, the content detection is carried out by utilizing a liquid phase mass spectrum, and the influence of different antioxidants on the thermal storage stability is screened according to the content of the allicin.

And (3) test design: 5% allicin seed treatment (microcapsule suspension), 5% allicin 01,5% allicin 02,5% allicin 03,5% allicin 04,5% allicin 05,5% allicin 06,5% allicin 07,5% allicin 08,5% allicin seed treatment (aqueous emulsion), 5% allicin 09,5% allicin 10,5% allicin 11,5% allicin 12,5% allicin 13,5% allicin 14,5% allicin 15,5% allicin 16, specific formulations are referred to in table 21.

The detection method comprises the following steps: dissolving with methanol, taking acetonitrile and water as mobile phases, using a stainless steel column with C18 and 5 mu m as fillers and an ultraviolet detector with variable wavelength, and carrying out reversed-phase high-performance liquid chromatography separation and determination on allicin (calculated by the sum of diallyl disulfide (2S) and diallyl trisulfide (3S)) in a sample, and quantifying by an external standard method.

Table 21 test treatment

The test results are shown in the following table.

TABLE 22 detection results of different treatment contents

Example 8

Effect of different antioxidants on allicin growth-promoting test

And (3) test design: the specific formulation is referred to in Table 21.

The test method comprises the following steps: the garlicin was formulated into a 5% formulation in accordance with the concept of example 7 and then stored at 54℃for 14d, with specific reference to the test procedure of example 3. ( Description: samples of the freshly configured 5% allicin preparation for examples 1-6 )

TABLE 23 influence of different treatments on plant height

TABLE 24 Effect of different treatments on root length

TABLE 25 influence of different treatments on plant fresh weight

TABLE 26 Effect of different treatments on root fresh weight

Example 9

Influence of the addition of different antioxidants on the inhibition of the corn sheath blight by allicin

And (3) test design: 5% allicin seed treatment agent (aqueous emulsion: added oxidant BHT); 5% allicin seed treatment agent (aqueous emulsion: added oxidant D-alpha tocopherol); 5% allicin seed treatment agent (microcapsule suspending agent: added oxidant BHT); 5% allicin seed treatment agent (microcapsule suspending agent: added oxidant D-alpha tocopherol). A6% allicin emulsion in water and fludioxonil (25 g/L) were used as positive controls. Meanwhile, clean water is used as CK contrast.

The test method comprises the following steps: the garlicin was formulated into a 5% formulation in accordance with the concept of example 7 and then stored at 54℃for 14d, with specific reference to the test procedure of example 5.

Table 27 control of maize sheath blight by different treatments

Table 28 control of maize sheath blight by different treatments

The result shows that the D-alpha tocopherol can be used as an optimal auxiliary material for the allicin antioxidant, can reduce the decomposition of the allicin at high temperature, and simultaneously plays the growth promoting effect of the allicin on plants and the inhibition effect of the allicin on pathogenic bacteria to the greatest extent.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The seed treating agent containing garlicin is characterized by comprising 0.5-20% of garlicin, 0.5-15% of solvent, 0.5-20% of emulsifier, 0.05-1% of antioxidant, 0.5-15% of film forming agent, 0.5-20% of dispersing agent, 0.5-20% of microcapsule material, 0.5-15% of warning color, 0.5-20% of antifreeze agent and 0.01-0.1% of stabilizing agent.

2. The seed treatment agent according to claim 1, comprising 1 to 10% of allicin, 1 to 10% of a solvent, 1 to 10% of an emulsifier, 0.05 to 0.5% of an antioxidant, 1 to 10% of a film forming agent, 1 to 10% of a dispersing agent, 1 to 10% of a microcapsule material, 1 to 10% of a warning color, 1 to 10% of an antifreeze agent, and 0.01 to 0.05% of a stabilizer.

3. The seed treatment agent according to claim 2, comprising 2 to 7% of allicin, 1 to 5% of solvent, 2 to 7% of emulsifier, 0.1 to 0.4% of antioxidant, 2 to 7% of film forming agent, 2 to 7% of dispersing agent, 2 to 7% of microcapsule material, 1 to 5% of warning color, 2 to 7% of antifreeze agent and 0.01 to 0.05% of stabilizer.

4. A seed treatment agent according to any one of claims 1 to 3, wherein the solvent is selected from the group consisting of an alkane solvent comprising at least one of mineral spirits, benzene, toluene, xylene, trimethylbenzene, and mixed benzene.

5. A seed treatment as claimed in any one of claims 1 to 3, wherein the emulsifier comprises at least one of the following components: castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, phenethyl phenol polyoxyethylene ether and calcium dodecyl benzene sulfonate.

6. A seed treatment as claimed in any one of claims 1 to 3, wherein the antioxidant comprises at least one of the following components: d-alpha tocopherol, methyl-esterified soybean oil, rosemary essential oil, epigallocatechin gallate, phytic acid, propyl gallate, BHT, tea polyphenol, ascorbic acid, BHA, epoxidized soybean oil; further D-alpha tocopherol.

7. A seed treatment as claimed in any one of claims 1 to 3, wherein the film former comprises at least one of the following components: polyvinyl alcohol, polyvinyl acetate, carboxymethyl cellulose, gum arabic, gelatin, xanthan gum, starch, microcrystalline cellulose, polyethylene glycol, polystyrene-acrylic emulsion, polyvinylpyrrolidone, polyvinyl acetate.

8. A seed treatment as claimed in any one of claims 1 to 3, wherein the dispersant comprises at least one of the following components: phosphate dispersants, sodium methyl naphthalene sulfonate formaldehyde condensate, alkylaryl sulfonate, alpha-alkenyl sulfonate, sodium dodecyl sulfate, succinate sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, alkylphenol ethoxylates, polystyrylphenol ethoxylates, castor oil ethoxylates, sorbitan fatty acid esters, sorbitan fatty acid ester ethoxylates, ethylene oxide propylene oxide block copolymers, phenolic resin polyoxyalkylene ethers, fatty alcohol polyoxyethylene ethers, fatty acid polyoxyalkylene ether esters, fatty amine polyoxyalkylene ethers.

9. A seed treatment as claimed in any one of claims 1 to 3, wherein the microcapsule material comprises at least one of the following components: capsule wall material, polyurea, polyamide, polyurethane, polyester, urea-formaldehyde resin, polystyrene, polyurethane, melamine resin.

10. A seed treatment as claimed in any one of claims 1 to 3, wherein the alert colour comprises at least one of the following components: acid scarlet, rose bengal, rose essence, carmine, congo red.

11. A seed treatment as claimed in any one of claims 1 to 3, wherein the antifreeze agent comprises at least one of the following components: ethylene glycol, glycerol, urea, propylene glycol, polyethylene glycol, and sorbitol.

12. A seed treatment as claimed in any one of claims 1 to 3, wherein the stabiliser comprises at least one of the following components: diutan, guar gum, xanthan gum, sodium carboxymethyl cellulose, sodium polyacrylate, diatomite, bentonite, attapulgite, light calcium carbonate, white carbon black and clay.

13. A seed treatment according to any one of claims 1 to 3, wherein the seed treatment is beneficial for plant growth and/or protects plants from plant pests; the plant pest is at least one selected from plant fungal pathogens, plant bacterial pathogens, nematodes and insects.

14. The seed treatment of claim 13, wherein the plant is selected from at least one of corn, soybean, potato, cotton, tomato, pepper, melon, sugarcane, peanut, wheat.

15. A seed treatment as claimed in any one of claims 1 to 3, wherein the plant treatment is in the form of a suspension seed coating, an aqueous emulsion, a microcapsule suspension, a dispersible powder, a dry mix, a microemulsion; further an aqueous emulsion and a microcapsule suspending agent.

16. Use of a seed treatment according to any one of claims 1 to 15 for plant growth and/or for protecting plants from plant pests; the plant pest is at least one selected from plant fungal pathogens, plant bacterial pathogens, nematodes and insects.

17. A method for benefiting plant growth and/or protecting a plant from a plant pest, the method comprising: plant seeds are treated with a seed treatment according to any one of claims 1 to 16.