CN117296846A - Attractant composition and application thereof - Google Patents
Attractant composition and application thereof Download PDFInfo
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- CN117296846A CN117296846A CN202311254854.8A CN202311254854A CN117296846A CN 117296846 A CN117296846 A CN 117296846A CN 202311254854 A CN202311254854 A CN 202311254854A CN 117296846 A CN117296846 A CN 117296846A
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- cis
- methyl
- butanol
- tetradecene acetate
- tetradecene
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- 239000005667 attractant Substances 0.000 title claims abstract description 20
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 19
- 239000000203 mixture Substances 0.000 title claims abstract description 19
- MXLMTQWGSQIYOW-UHFFFAOYSA-N 3-methyl-2-butanol Chemical compound CC(C)C(C)O MXLMTQWGSQIYOW-UHFFFAOYSA-N 0.000 claims abstract description 74
- 229940095068 tetradecene Drugs 0.000 claims abstract description 61
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims abstract description 60
- 241000199694 Harmonia axyridis Species 0.000 claims abstract description 25
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 35
- 241000256251 Spodoptera frugiperda Species 0.000 claims description 13
- 239000002904 solvent Substances 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 26
- 239000000243 solution Substances 0.000 description 29
- 241000607479 Yersinia pestis Species 0.000 description 13
- 241000255749 Coccinellidae Species 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 7
- 239000000877 Sex Attractant Substances 0.000 description 6
- 230000004044 response Effects 0.000 description 6
- 239000011259 mixed solution Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000256250 Spodoptera littoralis Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000003620 semiochemical Substances 0.000 description 3
- 241001480224 Heterodera Species 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000008786 sensory perception of smell Effects 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000545417 Aleurites Species 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241001414720 Cicadellidae Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241001604845 Heterodera sinensis Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000019439 ethyl acetate Nutrition 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000010832 independent-sample T-test Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
Abstract
The invention discloses an attractant composition, which is prepared from cis-9-tetradecene acetate and 3-methyl-2-butanol according to the mass ratio of (1-4): (1-4). The invention discovers that cis-9-tetradecene acetate has an attracting effect on female and male of the harmonia axyridis for the first time, 3-methyl-2-butanol can increase the attracting effect of cis-9-tetradecene acetate on the harmonia axyridis, and 3-methyl-2-butanol also has a certain attracting effect on the harmonia axyridis. Therefore, the cis-9-tetradecene acetate or the cis-9-tetradecene acetate and 3-methyl-2-butanol composition can attract the heteroladybug in a local area, so that the population density of the heteroladybug is improved, the field heteroladybug utilization rate can be improved, and the biological control efficiency of the heteroladybug can be enhanced.
Description
Technical Field
The invention relates to the technical field of attractants, in particular to an attractant composition and application thereof.
Background
Spodoptera frugiperda is one of ten world pests and has great harm to crops. With the diffusion and invasion of spodoptera frugiperda in China, the method has a great threat to the grain safety in China. The harmonia axyridis (Harmonia axyridis) belongs to the family of Coleoptera and is an important predatory natural enemy insect and is widely distributed worldwide. The development calendar of the harmonia axyridis passes through 4 stages of eggs, larvae, pupae and adults, and has the characteristics of large appetite, long service life, strong adaptability and fertility and the like. The harmonia axyridis can prey on various agricultural pests such as aphids, lepidoptera insect eggs and larvae, leafhoppers, whiteflies and the like, and is an important ecological factor for playing the service function of a farmland ecological system. The harmonia axyridis can exert higher predatory potential in many habitats and plays an important role in biological control of pests.
The localization of natural enemy insects to hosts or prey mainly uses semiochemicals as cues. The harmonia axyridis is an important predatory natural enemy of spodoptera frugiperda. Besides artificial propagation release, the utilization way of the harmonia axyridis is also an important means for trapping and collecting semiochemicals released by agricultural pests. Agricultural pest semiochemicals are micro-chemical substances secreted by pests and released to the outside of the body, can regulate the behaviors of the pests and natural enemies, and are the most important ways for the natural enemies to locate the pests or the habitat of the pests. Spodoptera frugiperda is a crop pest and disease damage delimited by agricultural rural areas, the theoretical maximum food catching capacity of spodoptera frugiperda 1-year larvae is 249, and female adult of the spodoptera frugiperda has stronger predatory capacity on spodoptera frugiperda larvae. Has stronger prevention and control capability on spodoptera frugiperda larvae.
By utilizing the specificity of the insects, the attractant can effectively attract the corresponding insects, thereby effectively destroying the reproduction of the insects and controlling the population thereof. Therefore, the sex pheromone of the major agricultural pest spodoptera littoralis is utilized to develop behavior regulation research on the sex pheromone, and the development of the behavior regulator for accurately positioning important pests such as spodoptera littoralis by the heterocarpa, is beneficial to improving the control effect on the pests such as spodoptera littoralis.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide an attractant composition and application thereof, and the sex pheromone component cis-9-tetradecene acetate of spodoptera frugiperda is found to have obvious attracting effect on predatory natural enemy of the spodoptera frugiperda by using an antenna potential EAG and Y-type pipe olfaction measuring technology, and the attracting effect of the cis-9-tetradecene acetate on the heterocarpa can be obviously improved by adding 3-methyl-2-butanol.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the invention provides a first aspect of an attractant composition for a ladybug, which is prepared from cis-9-tetradecene acetate and 3-methyl-2-butanol according to the mass ratio of (1-4): (1-4).
Preferably, the mass ratio of the cis-9-tetradecene acetic ester to the 3-methyl-2-butanol is 1:1.
the second aspect of the invention provides the use of an attractant composition as described above for attracting a ladybug.
Preferably, the attractant composition is used with n-hexane as a solvent, the concentration of cis-9-tetradecene acetate in the attractant composition is 0.001 μg/μl to 0.1 μg/μl, and the concentration of 3-methyl-2-butanol in the attractant composition is 0.001 μg/μl to 0.1 μg/μl.
Preferably, the ladybug includes female and male individuals.
Sex pheromone has the advantages of specificity, low concentration, high efficiency, no toxicity and the like. The invention discovers that the sex pheromone component cis-9-tetradecene acetate of spodoptera frugiperda has obvious attraction effect on predatory natural enemy heterodera sinensis. The addition of 3-methyl-2-butanol can obviously improve the attracting effect of cis-9-tetradecene acetate on the harmonia axyridis, and the 3-methyl-2-butanol also has a certain attracting effect on the harmonia axyridis. The invention combines cis-9-tetradecene acetate and 3-methyl-2-butanol for use, and has synergistic promotion effect in improving the attracting effect of the harmonia axyridis.
The invention has the beneficial effects that:
the invention discovers that cis-9-tetradecene acetate has an attracting effect on female and male of the harmonia axyridis for the first time, 3-methyl-2-butanol can increase the attracting effect of cis-9-tetradecene acetate on the harmonia axyridis, and 3-methyl-2-butanol also has a certain attracting effect on the harmonia axyridis. Therefore, the cis-9-tetradecene acetate or the cis-9-tetradecene acetate and 3-methyl-2-butanol composition can attract the heteroladybug in a local area, so that the population density of the heteroladybug is improved, the field heteroladybug utilization rate can be improved, and the biological control efficiency of the heteroladybug can be enhanced.
Drawings
Fig. 1: absolute EAG response value of cis-9-tetradecene acetate to female and male tentacles of the harmonia axyridis;
fig. 2: the cis-9-tetradecene acetate reacts on the Y-type tube of the female and male of the harmonia axyridis in an olfactory manner;
fig. 3: the attraction effect of the composition of cis-9-tetradecene acetate and 3-methyl-2-butanol on the harmonia axyridis.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
As described in the background art, in order to develop the attractant with excellent effect on the heteroladybug, the sex pheromone component cis-9-tetradecene acetate of spodoptera frugiperda is found to have obvious attracting effect on predatory natural enemy heteroladybug by using the antenna potential EAG and Y-type pipe olfaction measuring technology.
Preparing a cis-9-tetradecene acetate solution:
taking 10 mu L (density 0.87 g/mL) of cis-9-tetradecene acetate as a solvent, fixing the volume to 8.70mL of total volume, and uniformly mixing to obtain a cis-9-tetradecene acetate solution with the concentration of 1 mu g/mu L. 100. Mu.L of 1. Mu.g/. Mu.L of cis-9-tetradecene acetate solution was mixed with 900. Mu.L of n-hexane to obtain a cis-9-tetradecene acetate solution having a concentration of 0.1. Mu.g/. Mu.L. 100. Mu.L of 0.1. Mu.g/. Mu.L of cis-9-tetradecene acetate solution was mixed with 900. Mu.L of n-hexane to give a cis-9-tetradecene acetate solution having a concentration of 0.01. Mu.g/. Mu.L. 100. Mu.L of 0.01. Mu.g/. Mu.L of cis-9-tetradecene acetate solution was mixed with 900. Mu.L of n-hexane to obtain a cis-9-tetradecene acetate solution having a concentration of 0.001. Mu.g/. Mu.L. 100. Mu.L of 0.001. Mu.g/. Mu.L of cis-9-tetradecene acetate solution was mixed with 900. Mu.L of n-hexane to give a cis-9-tetradecene acetate solution having a concentration of 0.0001. Mu.g/. Mu.L.
Feeler potential test:
female and male adult ladybug were selected as subjects. Female and male tentacles of the harmonia axyridis were individually cut from the base using an ophthalmic surgical scissors, and each dissected tentacle was immediately fixed to 2 metal electrodes with an electrode gel (Spectra 360 electrode gel). 5 concentrations (0.0001, 0.001, 0.01, 0.1, 1. Mu.g/. Mu.L) of the cis-9-tetradecene acetate solution prepared above and 10. Mu.L of n-hexane (blank) were each dropped onto a 0.5X15 cm filter strip, and the filter strips were placed in 1mL Pasteur tubes, respectively, as a stimulus for use. The observation instrument displays the baseline condition, after waiting for the baseline to be stable, the pedal is stepped on to stimulate the antenna, the stimulation controller releases 0.5s of air at the speed of 0.4mL/s, and a 15s interval is allowed between continuous stimulation for the antenna to restore the stable state. 15 antenna EAG recordings are required for each stimulus. Signal storage and analysis used EAG ver.2.5 software (Synthesis, hilversum, netherlands).
The absolute reaction value calculation formula of the ladybug antenna EAG comprises the following steps: absolute EAG response value = test odor response value-control response value. Independent sample t-test analysis was performed on EAG absolute response values of the female and male treated groups and the control group, respectively, by SPSS Statistics 20.0 (IBM Crop, armonk, new York). The experimental results are shown in FIG. 1.
The results in FIG. 1 show that 5 concentrations of cis-9-tetradecene acetate solution can cause significant electrophysiological reactions on the antenna of male and female heteroladybug, indicating that both female and male heteroladybug can sense cis-9-tetradecene acetate.
Y-tube olfactory assay:
y-tube olfactometer (middle main arm: length 20.0cm, diameter 1.5cm; two side arms: length 15.0cm, diameter 1.5cm, included angle 75 °). The two arms are connected to a flow meter with teflon tubing, respectively, and air is pumped through an activated carbon filter and re-humidified by a bottle containing deionized water and then directedInto the 2 arms of the olfactometer. The behavioral response of female and male adult ladybug to the 5 different concentrations (0.0001, 0.001, 0.01, 0.1, 1. Mu.g/. Mu.L) of cis-9-tetradecene acetate solution was determined using n-hexane as a control. mu.L of each test solution was applied to a filter paper strip (2X 1 cm) 2 ) After the solvent had evaporated for 1min, the filter paper strips were inserted into an odor source glass bottle attached to one arm of the olfactometer. A control glass bottle connected to the other end of the olfactometer was filled with a 10. Mu.L strip of n-hexane treated filter paper. The selection of crossing half arms by the harmonia axyridis within 5min was considered reactive and the data was recorded. Insects that did not make a selection within 5min were considered to be unresponsive. 25 valid data were recorded for each treatment, and no data were recorded for individuals who did not respond. The olfactometer was rotated 90 ° after each test to avoid any directional deviation. After 5 times of repetition, the olfactory instrument is thoroughly cleaned and rinsed by acetone, and the whole testing process is carried out in a darkroom, so that the influence of uneven surrounding environment light sources is avoided. By using χ 2 The test performs a statistical analysis of the number of selections. The experimental results are shown in FIG. 2.
The results of FIG. 2 show that 0.0001-0.1 μg/μl of cis-9-tetradecene acetate is capable of significantly attracting adult male heterodera axyridis; 0.001-0.1 mug/mug of cis-9-tetradecene acetate has an attracting effect on female adult heterodera. In summary, 0.001-0.1 mug/mug of cis-9-tetradecene acetate has the best attracting effect on the adult of the harmonia axyridis.
In order to enable those skilled in the art to more clearly understand the technical solutions of the present application, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and are commercially available.
Example 1
Preparation of the attractant:
(1) Preparing a cis-9-tetradecene acetate solution:
taking 10 mu L (density 0.87 g/mL) of cis-9-tetradecene acetate as a solvent, fixing the volume to 8.70mL of total volume, and uniformly mixing to obtain a cis-9-tetradecene acetate solution with the concentration of 1 mu g/mu L. 100. Mu.L of 1. Mu.g/. Mu.L of cis-9-tetradecene acetate solution was mixed with 900. Mu.L of n-hexane to obtain a cis-9-tetradecene acetate solution having a concentration of 0.1. Mu.g/. Mu.L. 100. Mu.L of 0.1. Mu.g/. Mu.L of cis-9-tetradecene acetate solution was mixed with 900. Mu.L of n-hexane to give a cis-9-tetradecene acetate solution having a concentration of 0.01. Mu.g/. Mu.L.
(2) Preparation of 3-methyl-2-butanol solution: 10. Mu.L (density 0.818 g/mL) of 3-methyl-2-butanol is taken by taking normal hexane as a solvent, the volume is fixed to 8.18mL of the total volume, and the 3-methyl-2-butanol solution with the concentration of 1. Mu.g/mu.L is obtained after uniform mixing. 100. Mu.L of a 1. Mu.g/. Mu.L 3-methyl-2-butanol solution was mixed with 900. Mu.L of n-hexane to give a 3-methyl-2-butanol solution having a concentration of 0.1. Mu.g/. Mu.L. 100. Mu.L of a 0.1. Mu.g/. Mu.L 3-methyl-2-butanol solution was mixed with 900. Mu.L of n-hexane to give a 3-methyl-2-butanol solution having a concentration of 0.01. Mu.g/. Mu.L.
A mixed solution of cis-9-tetradecene acetate and 3-methyl-2-butanol in a mass ratio of 1:1 was prepared by mixing 0.01. Mu.g/. Mu.L of cis-9-tetradecene acetate and 0.01. Mu.g/. Mu.L of 3-methyl-2-butanol each 2 mL.
Experimental example
Attraction test:
y-tube olfactometer (middle main arm: length 20.0cm, diameter 1.5cm; two side arms: length 15.0cm, diameter 1.5cm, included angle 75 °). The two arms were each connected to a flow meter with a teflon tube, air was pumped through an activated carbon filter and re-humidified by a bottle of deionized water before being directed into the 2 arms of the olfactometer. The behavioural selection response of adult female and male heteroladybug was tested against a mixed solution of 0.01. Mu.g/. Mu.L of cis-9-tetradecene acetate, 0.01. Mu.g/. Mu.L of 3-methyl-2-butanol, 0.01. Mu.g/. Mu.L of cis-9-tetradecene acetate and 0.01. Mu.g/. Mu.L of 3-methyl-2-butanol at a mass ratio of 1:1 with respect to n-hexane. mu.L of each test solution was applied to a filter paper strip (2X 1 cm) 2 ) After the solvent had evaporated for 1min, the filter paper strips were inserted into an odor source glass bottle attached to one arm of the olfactometer. A control glass bottle connected to the other end of the olfactometer was filled with a 10. Mu.L strip of n-hexane treated filter paper. Making the ladybug with a color of 5minThe selection of crossing half arms was considered reactive and the data recorded. Insects that did not make a selection within 5min were considered to be unresponsive. 25 valid data were recorded for each treatment, and no data were recorded for individuals who did not respond. The olfactometer was rotated 90 ° after each test to avoid any directional deviation. After 5 times of repetition, the olfactory instrument is thoroughly cleaned and rinsed by acetone, and the whole testing process is carried out in a darkroom, so that the influence of uneven surrounding environment light sources is avoided. By using χ 2 The test performs a statistical analysis of the number of selections.
The result of FIG. 3 shows that 0.01 μg/μl of 3-methyl-2-butanol has a certain attraction effect on the Aleurites variegatus and has a greater attraction effect on females than males; whereas a mixed solution of 0.01 mug/mug of cis-9-tetradecene acetate and 0.01 mug/mug of 3-methyl-2-butanol in a mass ratio of 1:1 can extremely remarkably attract female and male adults of the heterochrome ladybug, the attraction effect of the cis-9-tetradecene acetate and 3-methyl-2-butanol in the mass ratio of 1:1 on the adult heterochrome ladybug is remarkably enhanced compared with that of the cis-9-tetradecene acetate in a mass ratio of 0.01 mug/mug. It was shown that 0.01. Mu.g/. Mu.L of cis-9-tetradecene acetate and 0.01. Mu.g/. Mu.L of 3-methyl-2-butanol in a mass ratio of 1:1 increased the attracting effect of cis-9-tetradecene acetate on the isoladybug.
And (3) field experiment:
the experiment is provided with 3 treatment groups and 1 blank control group, wherein the first treatment group is a mixed solution of 0.01 mug/mug of cis-9-tetradecene acetate and 0.01 mug/mug of 3-methyl-2-butanol with the mass ratio of 1:1; the second treatment group was 0.01. Mu.g/. Mu.L of cis-9-tetradecene acetate; the third treatment group was 0.01. Mu.g/. Mu.L of 3-methyl-2-butanol. Adding each group of solutions into the trapping cores, adding n-hexane into the trapping cores of the blank control group, and placing the trapping cores into the trap after the n-hexane volatilizes, wherein the addition amount of each group of solutions is the same; each trap was spaced 6m apart and randomly arranged. The suspension height of the trap was 0.2m higher than that of the canopy of the plant, the number of individual ladybug in the trap was counted on the 10 th day after the placement, and the experimental results are shown in table 1.
Table 1 effects of field experiments on the attraction of groups of ladybug
The results in Table 1 show that the mixed solution of cis-9-tetradecene acetate and 3-methyl-2-butanol with the mass ratio of 1:1 can extremely remarkably attract female and male adults of the harmonia axyridis, and the cis-9-tetradecene acetate and the 3-methyl-2-butanol have a synergistic promotion effect in improving the attracting effect of the harmonia axyridis.
The foregoing description is only of the preferred embodiments of the present application and is not intended to limit the same, but rather, various modifications and variations may be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.
Claims (6)
1. An attractant composition, which is characterized by comprising cis-9-tetradecene acetate and 3-methyl-2-butanol according to the mass ratio of (1-4): (1-4).
2. An attractant composition according to claim 1, wherein the mass ratio of cis-9-tetradecene acetate to 3-methyl-2-butanol is 1:1.
3. use of the attractant composition of claim 1 or 2 for attracting harmonia axyridis.
4. Use according to claim 3, wherein the attractant composition is used in a concentration of 0.001 μg/μl to 0.1 μg/μl and 3-methyl-2-butanol to 0.001 μg/μl in the attractant composition using n-hexane as solvent.
5. The use according to claim 3, wherein the heteroladybug comprises female and male individuals.
6. Use of an attractant composition according to claim 1 or 2 for biological control of spodoptera frugiperda.
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| CN202311254854.8A CN117296846A (en) | 2023-09-27 | 2023-09-27 | Attractant composition and application thereof |
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| CN202311254854.8A CN117296846A (en) | 2023-09-27 | 2023-09-27 | Attractant composition and application thereof |
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