CN117282356A

CN117282356A - Fluid system

Info

Publication number: CN117282356A
Application number: CN202311576920.3A
Authority: CN
Inventors: 沈玥; 江湘儿; 梁承龙; 蔡冬; 张虎; 蔡健身; 刘俞斌
Original assignee: Changzhou New Yichan Life Technology Co ltd; BGI Shenzhen Co Ltd
Current assignee: Changzhou New Yichan Life Technology Co ltd; BGI Shenzhen Co Ltd
Priority date: 2023-11-24
Filing date: 2023-11-24
Publication date: 2023-12-26
Anticipated expiration: 2043-11-24
Also published as: CN117282356B

Abstract

A fluid system is disclosed. The fluid system comprises a reaction assembly and a cleaning assembly, wherein the reaction assembly comprises a reaction reagent source, a first liquid path and a reaction device, the reaction reagent source is used for providing a reaction reagent, and the reaction reagent source is communicated with a reaction cavity of the reaction device through the first liquid path. The cleaning component comprises a cleaning reagent source, a second liquid path and a power source, wherein the cleaning reagent source is used for providing a cleaning reagent, the cleaning reagent source is selectively communicated with the first liquid path through the second liquid path, the communicating part of the second liquid path and the first liquid path is close to the reaction reagent source, and the power source is used for enabling the cleaning reagent to sequentially flow through the second liquid path and the first liquid path. The fluid system realizes the automatic cleaning of the first liquid path, avoids the first liquid path blocking phenomenon caused by long-time residual crystallization of the reaction reagent in the first liquid path, has high cleaning efficiency, needs less manual operation and reduces labor cost.

Description

Fluid system

Technical Field

The invention relates to the technical field of biochemical reactions, in particular to a fluid system.

Background

The existing fluid system has a plurality of pipelines for adding the reagent, if the pipelines are not used for a long time, the reagent remained in the pipelines is contacted with air, and the reagent is easily crystallized in the pipelines to cause a blocking phenomenon, so that the reagent pipelines need to be cleaned frequently.

The reaction reagent generally used in biochemical reaction, for example, nucleic acid synthesis reaction, the reagent used is more than ten, so that the reagent bottle involved is more than ten, at present, the cleaning mode of the pipeline is to manually replace the reagent bottle of more than ten reagents, in particular to replace the reagent bottle with cleaning reagent, and then manually click each pump valve of the control pipeline to enable the cleaning reagent in the reagent bottle to enter the pipeline, thereby cleaning the pipeline. However, this cleaning method requires a long cleaning agent, is costly in labor, is prone to misoperation, and cannot achieve effective immediate cleaning, thereby increasing production cost.

Disclosure of Invention

The invention aims to provide a fluid system which can automatically clean a pipeline through which a reagent flows, has high cleaning efficiency and requires low labor cost.

To achieve the purpose, the invention adopts the following technical scheme:

a fluid system, comprising: the reaction component comprises a reaction reagent source, a first liquid path and a reaction device, wherein the reaction reagent source is used for providing a reaction reagent, the reaction device is provided with a reaction cavity, and the reaction reagent source is communicated with the reaction cavity through the first liquid path; the cleaning assembly comprises a cleaning reagent source, a second liquid path and a power source, wherein the cleaning reagent source is used for providing cleaning reagent, the cleaning reagent source is selectively communicated with the first liquid path through the second liquid path, the communicating position of the second liquid path and the first liquid path is close to the reaction reagent source, and the power source is used for enabling the cleaning reagent to sequentially flow through the second liquid path and the first liquid path.

Preferably, the power source comprises an air source, an air path and a first switch valve, wherein the air source is used for providing air which does not react with the cleaning agent, the air source is communicated with the cleaning agent source through the air path, a first air outlet end of the air path is positioned in the cleaning agent source and above the liquid level of the cleaning agent, a liquid inlet end of the second liquid path extends to below the liquid level of the cleaning agent, and the first switch valve is arranged on the air path.

Preferably, the reactant source and the first liquid path are provided with a plurality of groups; the second liquid path is of a multi-layer structure with at least two liquid path layers, each liquid path layer comprises at least one diversion branch, each diversion branch is provided with at least one liquid inlet end and a plurality of liquid outlet ends, the liquid outlet ends of the liquid path layer of the upper layer are communicated with the liquid inlet ends of the diversion branches of the liquid path layer of the lower layer in a one-to-one correspondence manner, at least one liquid inlet end of the liquid path layer of the first layer is arranged in a one-to-one correspondence manner with at least one cleaning reagent source, and the liquid outlet ends of the liquid path layer of the last layer are communicated with the first liquid paths in a one-to-one correspondence manner; and/or, the gas circuit is a multi-layer structure with at least two gas circuit layers, each gas circuit layer comprises at least one gas distribution branch circuit, the gas distribution branch circuit is provided with at least one gas inlet end and a plurality of gas outlet ends, the gas outlet ends of the gas circuit layer of the upper layer are communicated with the gas inlet ends of the gas distribution branch circuit of the next layer, at least one gas inlet end of the first layer is communicated with at least one gas source in a one-to-one correspondence manner, and the gas outlet ends of the last layer extend into a plurality of reactant sources in a one-to-one correspondence manner.

Preferably, the gas source is communicated with the reactant source through the gas path and is used for introducing gas which does not react with the reactant into the reactant source, the second gas outlet end of the gas path is positioned in the reactant source and above the liquid level of the reactant, and the liquid inlet end of the first liquid path extends to below the liquid level of the reactant.

Preferably, the reaction reagent source is provided with a plurality of, the first liquid way includes first total liquid way and a plurality of first branch liquid way, one end of first total liquid way and a plurality of go out liquid end on first branch liquid way between through the diverter valve intercommunication, the other end on first total liquid way with the reaction chamber intercommunication, a plurality of the reaction reagent source and a plurality of the feed liquor end on first branch liquid way one-to-one intercommunication.

Preferably, a flowmeter is arranged on the first total liquid path; and/or a second switch valve is arranged on the first branch liquid path.

Preferably, the second liquid path includes a second total liquid path and a plurality of second branch liquid paths, liquid inlet ends of the second branch liquid paths are all communicated with liquid outlet ends of the second total liquid path, liquid inlet ends of the second total liquid path are communicated with the cleaning reagent source, and liquid outlet ends of the second branch liquid paths are respectively communicated with the first branch liquid paths in a one-to-one correspondence manner through a first switching valve.

Preferably, the second liquid path further comprises a third branch liquid path, a liquid inlet end of the third branch liquid path is communicated with a liquid outlet end of the second total liquid path, and a liquid outlet end of the third branch liquid path is communicated with one liquid inlet port of the switching valve.

Preferably, the fluid system further comprises a liquid discharge assembly comprising a liquid waste collection container and a first liquid waste discharge tube, the liquid discharge port of the reaction chamber being in selective communication with the liquid waste collection container through the first liquid waste discharge tube.

Preferably, the liquid draining assembly further comprises a second liquid draining pipe, one end of the second liquid draining pipe is selectively communicated with the first liquid path, the other end of the second liquid draining pipe is communicated with the liquid draining collecting container, and the communicating position of the second liquid draining pipe and the first liquid path is close to the reaction device.

The invention has the beneficial effects that:

the fluid system provided by the invention comprises a reaction component and a cleaning component, wherein the reaction component comprises a reaction reagent source, a first liquid path and a reaction device, the reaction reagent source is used for providing a reaction reagent, the reaction device is provided with a reaction cavity, and the reaction reagent source is communicated with the reaction cavity through the first liquid path. The cleaning component comprises a cleaning reagent source, a second liquid path and a power source, wherein the cleaning reagent source is used for providing a cleaning reagent, the cleaning reagent source is selectively communicated with the first liquid path through the second liquid path, the communicating part of the second liquid path and the first liquid path is close to the reaction reagent source, and the power source is used for enabling the cleaning reagent to sequentially flow through the second liquid path, the first liquid path and the reaction cavity. The fluid system can automatically inject the cleaning reagent into the first liquid path for the flowing of the reaction reagent by utilizing the cleaning component, and can automatically clean the first liquid path and the valve body arranged on the first liquid path by utilizing the cleaning reagent, thereby avoiding the occurrence of the first liquid path blocking phenomenon caused by long-time residual crystallization of the reaction reagent in the first liquid path, having high cleaning efficiency, requiring less manual operation and reducing labor cost.

Drawings

FIG. 1 is a schematic illustration of a fluid system provided in an embodiment of the present invention;

FIG. 2 is a schematic illustration of a portion of the structure of a fluid system provided in an embodiment of the present invention;

fig. 3 is a schematic view of a power source according to an embodiment of the present invention.

In the figure:

100. a reaction assembly; 110. a source of reactant; 120. a first liquid path; 121. a first total liquid path; 122. a first branch liquid path; 130. a reaction device; 140. a switching valve; 150. a flow meter; 160. a second switching valve; 170. a first switching valve;

200. cleaning the assembly; 210. cleaning a reagent source; 220. a second liquid path; 221. a second total liquid path; 222. a second branch liquid path; 223. a third branch liquid path; 230. a power source; 231. a gas source; 232. an air path; 233. a first switching valve; 234. a pressure regulating valve; 235. a pressure release valve; 236. a pressure reducing valve; 237. a first pressure gauge; 238. a second pressure gauge; 239. a first one-way valve; 2310. a second one-way valve; 240. a third switching valve;

300. a liquid discharge assembly; 310. a waste liquid collection container; 320. a first waste liquid discharge pipe; 330. a second switching valve; 340. a second waste liquid discharge pipe; 350. a third switching valve; 360. and a fourth switching valve.

Detailed Description

The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixed or removable, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

The specific structure of the fluid system according to the embodiment of the present invention is described below with reference to fig. 1 to 3.

The fluid system provided by the embodiment of the invention comprises a reaction assembly 100 and a cleaning assembly 200. The reaction assembly 100 is used for performing biochemical reactions, specifically, the reaction assembly 100 includes a reaction reagent source 110, a first liquid path 120 and a reaction device 130, where the reaction reagent source 110 is used for providing a reaction reagent, the reaction device 130 has a reaction chamber, the reaction chamber is a closed place where a reaction carrier reacts with the reaction reagent, and the reaction chamber has a liquid inlet and a liquid outlet that can be selectively opened and closed, and in some embodiments, the liquid inlet and the liquid outlet are the same opening. One end of the first liquid path 120 extends into the reaction reagent in the reaction reagent source 110, the other end is communicated with the liquid inlet of the reaction cavity, and the reaction reagent source 110 is communicated with the reaction cavity through the first liquid path 120, so that the reaction reagent can enter the reaction cavity through the first liquid path 120. In some embodiments, reaction assembly 100 is used to perform a nucleic acid synthesis reaction, reaction device 130 is a synthesis column, and the reagent is a synthesis reagent. Of course, in other embodiments, the reaction assembly 100 may be used to perform other types of biochemical reactions.

The cleaning assembly 200 is used for assisting in cleaning the first liquid path 120, specifically, the cleaning assembly 200 includes a cleaning agent source 210, a second liquid path 220 and a power source 230, the cleaning agent source 210 is used for providing a cleaning agent, one end of the second liquid path 220 extends into the cleaning agent in the cleaning agent source 210, the other end is selectively communicated with the first liquid path 120, the cleaning agent source 210 can be communicated with the first liquid path 120 through the second liquid path 220, and the communicating part of the second liquid path 220 and the first liquid path 120 is close to the reaction agent source 110 so as to clean the whole first liquid path 120 as much as possible. The power source 230 is used for providing power to enable the cleaning agent to sequentially flow through the second liquid path 220 and the first liquid path 120, and cleaning of the first liquid path 120 and the valve body arranged on the first liquid path 120 can be completed when the cleaning agent flows through the first liquid path 120.

Compared with the manual cleaning method in the prior art, in which the reaction reagent bottle needs to be disassembled and the cleaning reagent bottle needs to be replaced, the fluid system provided by the embodiment of the invention is provided with the cleaning assembly 200, and the cleaning reagent in the cleaning reagent source 210 flows into the first liquid path 120 through the second liquid path 220 under the driving of the power source 230, so that the cleaning of the first liquid path 120 and the valve body arranged on the first liquid path 120 is realized in the process. The fluid system provided by the embodiment of the invention realizes the automatic cleaning of the first liquid path 120, the consistency of the cleaning time and the cleaning performance can be effectively ensured, the contact time of the residual reagent in the first liquid path 120 with air is reduced, the blocking phenomenon of the first liquid path 120 caused by long-time residual crystallization of the reaction reagent in the first liquid path 120 is avoided, the replacement operation of a cleaning reagent bottle is not needed, the operation required by manpower is reduced, the labor cost is reduced, the occurrence of misoperation caused by insufficient experience or carelessness by manpower is avoided, the labor cost is reduced, and the cleaning efficiency is improved.

In some embodiments, with continued reference to FIG. 3, the power source 230 includes a gas source 231, a gas path 232, and a first on-off valve 233, the gas source 231 for providing a gas that does not react with the cleaning agent. The air source 231 is communicated with the cleaning agent source 210 through the air path 232, a first air outlet end of the air path 232 is positioned in the cleaning agent source 210 and above the liquid level of the cleaning agent, a liquid inlet end of the second liquid path 220 extends to below the liquid level of the cleaning agent, and the first switch valve 233 is arranged on the air path 232. As the gas is injected into the cleaning agent source 210, a high-pressure environment is formed above the cleaning agent, and the cleaning agent in the cleaning agent source 210 can automatically flow into the second liquid path 220 in the high-pressure environment formed by the gas.

In a specific embodiment, the gas source 231 is a high-pressure gas cylinder, and the high-pressure gas cylinder contains high-pressure gas that does not react with the cleaning agent, and optionally, the high-pressure gas is high-pressure air, helium, argon, nitrogen, or the like. In another specific embodiment, the air source 231 is an air compressor for providing air.

In a specific embodiment, the first switch valve 233 is a manual valve; of course, in other embodiments, the first switch valve 233 may be a solenoid valve.

With continued reference to FIG. 1, in some embodiments, a pressure gauge is provided on the air path 232, which may be one or more in number. In one particular embodiment, the gas circuit 232 is provided with a first pressure gauge 237 and a second pressure gauge 238, the first pressure gauge 237 being disposed downstream of the gas source 231 and configured to indicate the pressure of the gas within the gas circuit 232 just exiting the gas source 231, the second pressure gauge 238 being disposed downstream of the first switch valve 233 and configured to indicate the pressure within the gas circuit 232 downstream of the first switch valve 233.

In some embodiments, a pressure regulating valve 234 is provided on the air path 232. Alternatively, the pressure regulating valve 234 is disposed downstream of the first pressure gauge 237 and upstream of the first on-off valve 233, and is used to regulate the pressure of the gas flowing from the gas source 231 within a preset range. In some embodiments, the pressure regulating valve 234 is a solenoid valve, which is simple and convenient to control and can assist in achieving on-line control.

In some embodiments, a pressure relief valve 235 is provided on the air path 232. Optionally, a pressure relief valve 235 is disposed downstream of the pressure regulating valve 234 and upstream of the first on-off valve 233, the pressure relief valve 235 being configured to protect the entire gas system, and gas being vented from the pressure relief valve 235 when the pressure in the gas path 232 exceeds the pressure set by the pressure relief valve 235. In some embodiments, the pressure release valve 235 is a solenoid valve, which is simple and convenient to control and can assist in achieving on-line control.

In some embodiments, a pressure relief valve 236 is provided on the air path 232. Alternatively, a pressure reducing valve 236 is provided downstream of the pressure relief valve 235 and upstream of the first on-off valve 233, the pressure reducing valve 236 being adapted to reduce the pressure of the gas flowing from the gas source 231 to a desired pressure value. In some embodiments, the pressure reducing valve 236 is a solenoid valve, which is simple and convenient to control and can assist in achieving on-line control.

In some embodiments, the air path 232 is provided with a one-way valve for preventing the backflow of gas or reagent from the reagent bottle or the cleaning reagent bottle, and performing a reverse cut-off function. In a specific embodiment, two check valves, a first check valve 239 and a second check valve 2310, are disposed on the gas path 232, the first check valve 239 being disposed downstream of the first switch valve 233 and upstream of the second pressure gauge 238, and the second check valve 2310 being disposed downstream of the second pressure gauge 238 and upstream of the cleaning agent source 210.

Further, gas source 231 also provides power for reactant injection into the reaction chamber. In some embodiments, the gas source 231 is connected to the reactant source 110 through the gas path 232 and is used to introduce a gas that does not react with the reactant into the reactant source 110, the second gas outlet end of the gas path 232 is located in the reactant source 110 and above the liquid surface of the reactant, and the liquid inlet end of the first liquid path 120 extends below the liquid surface of the reactant. With the injection of the gas into the reactant source 110, a high pressure environment is formed above the reactant, and under the high pressure environment formed by the gas, the reactant in the reactant source 110 can automatically flow into the first liquid path 120 and finally enter the reaction chamber.

It should be noted that, the gas source 231 and the gas path 232 for providing power for the flow of the cleaning agent and the reaction agent may be the same group, or may be two groups independent of each other, when the gas source 231 and the gas path 232 are two groups, one group includes a first gas source and a first gas path for providing power for the cleaning agent, and the other group includes a second gas source and a second gas path for providing power for the reaction agent, and the gas in the first gas source and the gas in the second gas source may be the same or different, and may be flexibly set according to the requirement.

In some embodiments, the air path 232 has a structure with one air inlet end and a plurality of air outlet ends, and the air path branching block arranged at the branching position of the air path 232 is used for branching, so that one air source 231 can be used for simultaneously supplying air to the plurality of reaction reagent sources 110 and at least one cleaning reagent source 210, and external air can be prevented from entering while providing power for reagent flow, so that the risk of reagent crystallization is further reduced.

Of course, in other embodiments, a plurality of air sources 231 and a plurality of air paths 232 may be provided, such that each set of air sources 231 and air paths 232 supply a portion of the reactant sources 110, and all of the air sources 231 and air paths 232 together complete the supply of all of the reactant sources 110 and the cleaning agent sources 210.

Optionally, the air channel 232 may also be a multi-layer structure with at least two air channel layers, where each air channel layer includes at least one air distribution branch having at least one air inlet end and a plurality of air outlet ends, the air outlet ends of the air channel layer of the upper layer are in one-to-one correspondence with the air inlet ends of the air distribution branch of the air channel layer of the lower layer, the air inlet ends of the first layer are in communication with the air source 231, and the plurality of air outlet ends of the last layer extend into the plurality of reactant sources 110 in one-to-one correspondence. Specifically, the air channel 232 includes a plurality of air channel layers, where the air channel layer of the first layer has one air inlet end and at least two air outlet ends, so as to realize first division of the air channel 232 into multiple parts (for example, one division into two and one division into three), the air outlet end of the upper layer is used as the air inlet end of the lower layer, the air channel layer of the lower layer continues to divide the branches of the air channel layer of the upper layer into multiple parts (for example, one division into two and one division into three), and the above steps are repeated until the air channel of the last layer has enough air outlet ends. In one particular embodiment, the air paths 232 are laid out in one-half, two-half, four-quarter, eight-quarter, sixteen-eighth patterns.

In some embodiments, the cleaning reagent employs Acetonitrile (ACN), and the cleaning reagent source 210 is a cleaning reagent bottle containing acetonitrile. In some other embodiments, the cleaning agent is methylene chloride and the cleaning agent source 210 is a cleaning agent bottle containing methylene chloride. It should be noted that any cleaning agent that CAN be used, whether CAN or dichloromethane, is compatible with all reagents and does not affect the reagents.

Since there is generally more than one reagent involved in the biochemical reaction, it is necessary to provide a plurality of reagent sources 110, each of which is for containing the same or different nucleic acid reagents in the reagent source 110. To achieve communication between all of the reactant sources 110 and the reaction chamber, in some embodiments, the fluid system is provided with a plurality of first fluid paths 120 that are independent of each other, and each first fluid path 120 is provided with a power pump and a second switch valve 160, so as to achieve control over different first fluid paths 120, so that the reactants in different reactant sources 110 enter the reaction chamber. The power pump may also power the flow of reactant in first fluid path 120, where the gas source is no longer needed to supply gas into reactant source 110.

In some other embodiments, the first liquid path 120 includes a first total liquid path 121 and a plurality of first branch liquid paths 122, wherein one end of the first total liquid path 121 is communicated with the liquid outlet ends of the plurality of first branch liquid paths 122 through a switching valve 140, the other end of the first total liquid path 121 is communicated with the reaction chamber, and the plurality of reactant sources 110 are communicated with the liquid inlet ends of the plurality of first branch liquid paths 122 in a one-to-one correspondence.

Alternatively, the switching valve 140 is a rotary switching solenoid valve, and the rotary switching solenoid valve is used to switch to a different reagent port in a rotary manner, so that the first total liquid path 121 communicates with the first branch liquid path 122 of the target.

Further, in some embodiments, a flow meter 150 is disposed on the first total liquid path 121. The flow meter 150 functions to meter the injected amount of reagent while feeding back signals to the corresponding control mechanism to effect control of the amounts of reagent and cleaning reagent to be added.

In some embodiments, the first branch liquid path 122 is provided with a second switching valve 160. Optionally, the second switch valve 160 is a through electromagnetic valve, and when the second switch valve 160 is in a power-off state, the first branch liquid path 122 is closed; the second switching valve 160 is in an energized state, and the first branch liquid passage 122 is turned on. Of course, in other embodiments, the second on-off valve 160 may be a manual valve.

In order to enable the cleaning agent source 210 to be selectively communicated with any one of the plurality of first liquid paths 120, so that cleaning of all the first liquid paths 120 by using the cleaning agent of the cleaning agent source 210 can be completed, in some embodiments, the second liquid path 220 includes a second total liquid path 221 and a plurality of second branch liquid paths 222, liquid inlet ends of the plurality of second branch liquid paths 222 are all communicated with one end of the second total liquid path 221, the other end of the second total liquid path 221 is communicated with the cleaning agent source 210, and liquid outlet ends of at least part of the second branch liquid paths 222 are respectively communicated with the plurality of first branch liquid paths 122 in a one-to-one correspondence manner through one first switching valve 170. Alternatively, the first switching valve 170 is a two-position three-way solenoid valve.

Of course, in other embodiments, the second liquid path 220 may also be a multi-layer structure with at least two liquid path layers, each liquid path layer includes at least one split branch, the split branch has at least one liquid inlet end and a plurality of liquid outlet ends, the liquid outlet ends of the liquid path layer of the upper layer are in one-to-one correspondence with the liquid inlet ends of the split branches of the liquid path layer of the lower layer, at least one liquid inlet end of the first layer is in one-to-one correspondence with at least one cleaning agent source 210, and the liquid outlet ends of the last layer are in one-to-one correspondence with the first liquid paths 120. Specifically, the second liquid path 220 includes a plurality of liquid path layers, the liquid path layer of the first layer has one liquid inlet end and at least two liquid outlet ends, so as to realize first one-to-many (for example, one-to-two and one-to-three) of the second liquid path 220, the liquid outlet end of the upper layer is used as the liquid inlet end of the lower layer, the liquid path layer of the lower layer continues to perform one-to-many (for example, one-to-two and one-to-three) on the branch paths separated from the liquid path layer of the upper layer, and the steps are repeated until the liquid path of the last layer has enough liquid outlet ends. In a specific embodiment, the second fluid circuit 220 is laid out in the form of one-two, two-four, four-eight, eight-sixteen.

Optionally, a liquid path diversion block joint is provided at a communication position of the second total liquid path 221 and the plurality of second diversion liquid paths 222 or between two adjacent liquid path layers. The fluid path diversion quick connector is used for diverting the cleaning agent to the first plurality of switching valves 170, thereby facilitating switching of the cleaning agent lines.

The first switching valve 170 is used to switch flow paths of the reaction reagent and the washing reagent. Optionally, the first switching valve 170 is a three-way electromagnetic valve, the three-way electromagnetic valve includes an NO end and an NC end, when the three-way electromagnetic valve is in a power-off state, the NO end is normally open, the NC end is normally closed, the reactant flows to the corresponding second switching valve 160, when the three-way electromagnetic valve is in a power-on state, the NO end is switched to be closed, the NC end is switched to be opened, and the cleaning agent can reach the corresponding second switching valve 160.

In some embodiments, the second liquid path 220 further includes a third branch liquid path 223, a liquid inlet end of the third branch liquid path 223 is communicated with a liquid outlet end of the second total liquid path 221, and a liquid outlet end of the third branch liquid path 223 is communicated with one liquid inlet port of the switching valve 140. The third switching valve 240 is disposed on the third branch liquid path 223, and when the third switching valve 240 is in the on state, the cleaning agent directly flows to the switching valve 140 through the third branch liquid path 223 without passing through the first branch liquid path 122, and rapidly flows to the liquid discharging assembly 300 through the switching valve 140 and the first total liquid path 121, so as to achieve separate cleaning of the first total liquid path 121.

It should be noted that the fluid system has at least two cleaning conditions: the first method is that between adding different reagents in a certain biochemical reaction experiment, the first total liquid path 121 needs to be cleaned separately, so that the last reagent remaining in the first total liquid path 121 is cleaned, at this time, when the third switch valve 240 is in an on state, the cleaning reagent directly flows to the switch valve 140 through the third branch liquid path 223, and flows to the liquid discharge assembly 300 quickly through the switch valve 140 and the first total liquid path 121, so as to realize the separate cleaning of the first total liquid path 121; the second is that when the fluid system is not used for a long time, the first branch liquid path 122 and the first total liquid path 121 need to be cleaned uniformly, and when the third switch valve 240 is in the power-off state, the cleaning agent flows to the switching valve 140 through the first branch liquid path 122 to be cleaned, and flows to the liquid discharging assembly 300 after passing through the switching valve 140 and the first total liquid path 121, so that the first branch liquid path 122 and the first total liquid path 121 are cleaned integrally.

With continued reference to fig. 2, in some embodiments, the fluid system further includes a drain assembly 300, the drain assembly 300 including a waste collection container 310 and a first waste drain tube 320, the drain of the reaction chamber being in selective communication with the waste collection container 310 via the first waste drain tube 320. Optionally, a fourth switching valve 360 is provided on the first waste drain pipe 320. In one particular embodiment, the fourth switching valve 360 is a solenoid valve.

In some embodiments, one end of the first waste drain pipe 320 communicates with the first total liquid path 121 through the second switching valve 330, and the other end of the first waste drain pipe 320 communicates with the waste collection container 310. Optionally, the second switching valve 330 is a two-position three-way electromagnetic valve, the second switching valve 330 includes an NO end and an NC end, when the second switching valve 330 is in a power-off state, the NO end is normally open, the NC end is normally closed, the reagent in the first total liquid path 121 can flow into the reaction chamber, when the second switching valve 330 is in a power-on state, the NO end is switched to be closed, the NC end is switched to be opened, and the waste liquid in the reaction chamber can flow into the waste liquid collecting container 310 through the first waste liquid discharge pipe 320.

In some embodiments, the drain assembly 300 further includes a second drain tube 340, one end of the second drain tube 340 is in selective communication with the first fluid pathway 120, the other end of the second drain tube 340 is in communication with the waste collection container 310, and the junction of the second drain tube 340 and the first fluid pathway 120 is disposed proximate to the reaction device 130. This arrangement enables the washing reagent flowing out of the washing reagent bottle to be discharged directly into the waste liquid collecting container 310 without passing through the reaction device 130 after the washing of the first liquid path 120 is completed.

Further, in some embodiments, one end of the second waste drain pipe 340 communicates with the first total liquid path 121 through a third switching valve 350, the other end of the second waste drain pipe 340 communicates with the waste collection container 310, and the third switching valve 350 is disposed upstream of the second switching valve 330. Alternatively, the third switching valve 350 is a two-position three-way solenoid valve, the third switching valve 350 includes an NO end and an NC end, and the NO end of the third switching valve 350 communicates with the NC end of the second switching valve 330. When the third switching valve 350 is in the power-off state, the NO end is normally open, the NC end is normally closed, and the reagent in the first total liquid path 121 can flow to the second switching valve 330; when the third switching valve 350 is in the energized state, the NO end is switched to be closed, the NC end is switched to be opened, and the reagent in the first total liquid path 121 flows into the waste liquid collecting container 310 through the third switching valve 350 and through the second waste liquid discharge pipe 340.

It should be noted that, the arrangement of the second waste liquid discharge pipe 340 and the third switching valve 350 enables the cleaning solution after the cleaning agent cleans the first liquid path 120 (especially, between the different reagents added in any biochemical reaction experiment, the process of cleaning the first total liquid path 121 alone is needed) to be directly discharged into the waste liquid collecting container 310, so that the process of cleaning the first liquid path 120 and the process of performing biochemical reaction in the reaction chamber are independent and do not interfere with each other, and the normal operation of the reagents is prevented from being affected by the cleaning agent passing through the reaction chamber. In addition, when the liquid inlet and the liquid outlet of the reaction device 130 are two ports independent of each other, the second switching valve 330 may be omitted.

In addition, after the first total liquid path 121 is individually washed between the addition of different reagents in one of the biochemical reaction experiments, the first total liquid path 121 is filled with a washing reagent. Before the next reaction reagent is injected into the reaction chamber of the reaction device 130, the remaining cleaning reagent in the first total liquid path 121 needs to be replaced, and at this time, the first total liquid path 121 needs to be pre-injected. The process of pre-injection is as follows: the third switching valve 350 is controlled to be in an energized state, and the second switching valve 160 disposed on the first branch liquid path 122 corresponding to the reactant source 110 of the next reactant is controlled to be in an energized state, so that the next reactant flows into the first total liquid path 121 and drives the reactant in the first total liquid path 121 to flow into the second waste liquid discharge pipe 340 and finally discharge into the waste liquid collecting container 310, thereby completing the replacement of the cleaning agent in the first total liquid path 121, and avoiding the cleaning agent in the first total liquid path 121 from directly entering the reaction chamber of the reaction device 130 to affect the biochemical reaction.

As shown in fig. 2 and 3, the process of the priming purge will be described taking the first liquid path 120 and the switching valve 140 for the reactant shown in the drawings as an example:

the first switch valve 233 is opened, the third switch valve 350 is switched to the NC end, the second waste liquid discharge pipe 340 is communicated, the waste liquid discharge channel is opened, the switch valve 140 is switched to the port No. 7, the first total liquid path 121 is communicated with the first branch liquid path 122 of the reaction reagent, the first switch valve 170 is switched to the NC end, the cleaning reagent is connected, the second switch valve 160 is opened, the cleaning reagent enters the first branch liquid path 122 through the second liquid path 220, the flowmeter 150 starts to measure the cleaning reagent amount, the cleaning reagent finishes cleaning according to the cleaning amount, and the waste liquid collection container 310 is discharged, the second switch valve 160 is closed, the first switch valve 170 and the third switch valve 350 are powered off, so that the NO port is switched, the switch valve 140 is switched to the initial position (for example, the position No. 1) and the first liquid path 120 and the switch valve 140 are cleaned once.

The fluid system provided by the embodiment of the invention further comprises a control mechanism, and in the embodiment, the control mechanism can be a centralized or distributed controller, for example, the controller can be a single-chip microcomputer or a plurality of distributed single-chip microcomputers, and a control program can be run in the single-chip microcomputer to further control the valve bodies to realize the functions of the valve bodies. By controlling the on-off of different valve bodies, the cleaning of the plurality of first liquid paths 120 and the corresponding flow channels of the switching valve 140 can be realized in combination with the pre-injection cleaning reagent time sequence action of the different reagents previously set and written. In a word, this fluid system is not under the prerequisite that needs manual change reaction reagent bottle into the washing reagent bottle, can be on-line automatic through control above-mentioned valve body switch to corresponding washing reagent, realize the accurate notes liquid washing to the target liquid way.

It is to be understood that the above examples of the present invention are provided for clarity of illustration only and are not limiting of the embodiments of the present invention. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the invention are desired to be protected by the following claims.

Claims

1. A fluid system, comprising:

a reaction assembly (100), the reaction assembly (100) comprising a reaction reagent source (110), a first liquid path (120) and a reaction device (130), the reaction reagent source (110) being for providing a reaction reagent, the reaction device (130) having a reaction chamber, the reaction reagent source (110) being in communication with the reaction chamber through the first liquid path (120);

the cleaning assembly (200), the cleaning assembly (200) comprises a cleaning agent source (210), a second liquid path (220) and a power source (230), the cleaning agent source (210) is used for providing cleaning agent, the cleaning agent source (210) is selectively communicated with the first liquid path (120) through the second liquid path (220), the communicating position of the second liquid path (220) and the first liquid path (120) is close to the reaction agent source (110), and the power source (230) is used for enabling the cleaning agent to sequentially flow through the second liquid path (220) and the first liquid path (120).

2. The fluid system of claim 1 wherein the fluid system comprises a fluid,

the power source (230) comprises an air source (231), an air path (232) and a first switch valve (233), wherein the air source (231) is used for providing gas which does not react with the cleaning agent, the air source (231) is communicated with the cleaning agent source (210) through the air path (232), a first air outlet end of the air path (232) is positioned in the cleaning agent source (210) and above the liquid level of the cleaning agent, a liquid inlet end of the second liquid path (220) extends to below the liquid level of the cleaning agent, and the first switch valve (233) is arranged on the air path (232).

3. The fluid system of claim 2 wherein the fluid system comprises,

the reactant source (110) and the first liquid path (120) are provided with a plurality of groups;

the second liquid path (220) is of a multi-layer structure with at least two liquid path layers, each liquid path layer comprises at least one diversion branch, each diversion branch is provided with at least one liquid inlet end and a plurality of liquid outlet ends, the liquid outlet ends of the liquid path layer of the upper layer are communicated with the liquid inlet ends of the diversion branches of the liquid path layer of the lower layer in a one-to-one correspondence manner, at least one liquid inlet end of the liquid path layer of the first layer is arranged with at least one cleaning reagent source (210) in a one-to-one correspondence manner, and the liquid outlet ends of the liquid path layer of the last layer are communicated with the first liquid paths (120) in a one-to-one correspondence manner;

and/or, the gas circuit (232) is a multi-layer structure with at least two gas circuit layers, each gas circuit layer comprises at least one gas distribution branch, the gas distribution branch is provided with at least one gas inlet end and a plurality of gas outlet ends, the gas outlet ends of the gas circuit layer of the upper layer are communicated with the gas inlet ends of the gas distribution branch of the gas circuit layer of the lower layer in a one-to-one correspondence manner, the at least one gas inlet end of the gas circuit layer of the first layer is communicated with the at least one gas source (231) in a one-to-one correspondence manner, and the gas outlet ends of the gas circuit layer of the last layer extend into the reactant sources (110) in a one-to-one correspondence manner.

4. The fluid system of claim 2 wherein the fluid system comprises,

the gas source (231) is communicated with the reactant source (110) through the gas path (232) and is used for introducing gas which does not react with the reactant into the reactant source (110), the second gas outlet end of the gas path (232) is positioned in the reactant source (110) and above the liquid level of the reactant, and the liquid inlet end of the first liquid path (120) extends to below the liquid level of the reactant.

5. The fluid system of claim 1 wherein the fluid system comprises a fluid,

the reaction reagent source (110) is provided with a plurality of, first liquid way (120) is including first total liquid way (121) and a plurality of first branch liquid way (122), one end and a plurality of first total liquid way (121) go out liquid end between through switching valve (140) intercommunication, the other end of first total liquid way (121) with the reaction chamber intercommunication, a plurality of reaction reagent source (110) and a plurality of feed liquor end one-to-one intercommunication of first branch liquid way (122).

6. The fluid system of claim 5 wherein the fluid system comprises a fluid,

a flowmeter (150) is arranged on the first total liquid path (121);

and/or the first branch liquid path (122) is provided with a second switch valve (160).

7. The fluid system of claim 5 wherein the fluid system comprises a fluid,

the second liquid path (220) comprises a second total liquid path (221) and a plurality of second branch liquid paths (222), liquid inlet ends of the second branch liquid paths (222) are communicated with liquid outlet ends of the second total liquid path (221), liquid inlet ends of the second total liquid path (221) are communicated with the cleaning reagent source (210), and liquid outlet ends of the second branch liquid paths (222) are communicated with the first branch liquid paths (122) in a one-to-one correspondence mode through first switching valves (170).

8. The fluid system of claim 7 wherein the fluid system comprises a fluid,

the second liquid path (220) further comprises a third branch liquid path (223), a liquid inlet end of the third branch liquid path (223) is communicated with a liquid outlet end of the second total liquid path (221), and a liquid outlet end of the third branch liquid path (223) is communicated with one liquid inlet port of the switching valve (140).

9. The fluid system of claim 1 wherein the fluid system comprises a fluid,

the fluid system further comprises a liquid draining assembly (300), the liquid draining assembly (300) comprises a liquid waste collecting container (310) and a first liquid waste draining pipe (320), and a liquid draining port of the reaction cavity is selectively communicated with the liquid waste collecting container (310) through the first liquid waste draining pipe (320).

10. The fluid system of claim 9 wherein the fluid system comprises a fluid,

the liquid draining assembly (300) further comprises a second liquid draining pipe (340), one end of the second liquid draining pipe (340) is selectively communicated with the first liquid path (120), the other end of the second liquid draining pipe (340) is communicated with the liquid draining collection container (310), and the communicating part of the second liquid draining pipe (340) and the first liquid path (120) is close to the reaction device (130).