CN117269284B - Oxygen carrying-releasing capacity relative evaluation device - Google Patents
Oxygen carrying-releasing capacity relative evaluation device Download PDFInfo
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- CN117269284B CN117269284B CN202311558242.8A CN202311558242A CN117269284B CN 117269284 B CN117269284 B CN 117269284B CN 202311558242 A CN202311558242 A CN 202311558242A CN 117269284 B CN117269284 B CN 117269284B
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- oxygen
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- 239000001301 oxygen Substances 0.000 title claims abstract description 68
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 68
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 56
- 230000003578 releasing effect Effects 0.000 title claims abstract description 21
- 238000011156 evaluation Methods 0.000 title claims abstract description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 40
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 34
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 19
- 239000012530 fluid Substances 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims description 105
- 230000001105 regulatory effect Effects 0.000 claims description 21
- 238000010438 heat treatment Methods 0.000 claims description 11
- 230000001276 controlling effect Effects 0.000 claims description 8
- 239000007853 buffer solution Substances 0.000 claims description 7
- 238000004891 communication Methods 0.000 claims description 5
- 239000013078 crystal Substances 0.000 claims description 4
- 238000004088 simulation Methods 0.000 claims description 4
- 230000004044 response Effects 0.000 abstract description 5
- 230000010354 integration Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 50
- 239000000969 carrier Substances 0.000 description 9
- 102000001554 Hemoglobins Human genes 0.000 description 5
- 108010054147 Hemoglobins Proteins 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000010517 secondary reaction Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000006392 deoxygenation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- NBVXSUQYWXRMNV-UHFFFAOYSA-N fluoromethane Chemical compound FC NBVXSUQYWXRMNV-UHFFFAOYSA-N 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a relative evaluation device for oxygen carrying-oxygen releasing capacity, which belongs to the field of measuring and testing instruments, and comprises the following components: the flow cell module is used for detecting the oxygen partial pressure value of the to-be-detected product and comprises a fixed box and at least two flow cells, and the flow cells are connected to the outer wall of the fixed box; the peristaltic pump is connected inside the fixed box, connected with each flow cell tube and used for transmitting fluid in different flow cells; the device comprises a pneumatic control valve, a plurality of air tanks and a plurality of air tanks, wherein the pneumatic control valve is used for introducing one of nitrogen or air into each flow cell and is connected with each flow cell pipe; the circuit module is used for transmitting instructions for controlling the flow cell and comprises an upper computer and a circuit board which are connected, the upper computer is connected with the flow cell module, and the circuit board is electrically connected with the flow cell. By the arrangement, the time difference required by response between the running modules can be eliminated, the integration level of equipment can be improved, and the layout is compact.
Description
Technical Field
The invention belongs to the technical field of measuring and testing instruments, and particularly relates to a relative evaluation device for oxygen carrying-releasing capacity.
Background
Oxygen carrying-releasing properties are the most important functional indicators of various oxygen carriers (including natural oxygen carriers such as erythrocytes, hemoglobin, and artificial oxygen carriers), while the partial pressure of half oxygen saturation P50 is a key parameter characterizing this capability. At present, only HEMOXANALYZER produced by TSC Scientific company is internationally approved as an instrument for detecting P50, which draws an oxygen separation curve of a sample through the change of a characteristic spectrum of hemoglobin under visible light to obtain a P50 value of the sample, and natural hemoglobin, cross-linked or polymerized hemoglobin can be detected, but the instrument relates to an oxygenation and deoxygenation process, an optical component is also required to be regulated, and finally the P50 value is obtained according to the obtained oxygen separation curve, so that the detection operation is complicated, and a professional is usually required to operate the instrument, and a special buffer solution is required; the instrument is not suitable for evaluating the oxygen carrying/releasing functions of various artificial oxygen carriers such as fluorocarbon, ferriporphyrin analogues and the like, and the artificial oxygen carriers can not be detected because of colorless or absorption light wavelength ranges are no longer in the visible light range or because of too dark and opaque color. In addition, the blood gas instrument commonly used in clinic also detects the oxygen carrying-releasing capacity of blood through a characteristic spectrum, but when detecting different artificial oxygen carriers, the blood gas instrument can not well evaluate the important function index of the oxygen carrying-releasing capacity of the artificial oxygen carriers due to measurement errors caused by color interference.
The relative evaluation device of oxygen carrying-releasing ability currently used for solving the above problems has the following problems:
1. in the prior device, a plurality of valve bodies such as single pinch valves, regulating valves, switching valves and the like with different numbers exist, and when the device is used, an operator is required to manually control the on-off of the valve bodies through a peristaltic pump, so that time difference exists in response among all parts of the device, and the use efficiency of the device is low.
2. The current device comprises a circuit, a flow path and a flow cell, but the flow cell is small in size, and the circuit adopts an external layout, so that the overall integration level is low and hidden danger exists in use safety.
It is therefore desirable to design an apparatus based on existing devices that solves the above-mentioned technical problems, in particular an apparatus for the relative evaluation of oxygen carrying-releasing capacity.
Disclosure of Invention
In order to overcome the problems in the background art, the invention adopts the following technical scheme:
an oxygen carrying-oxygen releasing capacity relative evaluation device, comprising: the circulation module is used for detecting the oxygen partial pressure value of the to-be-detected product and comprises a fixed box and at least two circulation cells, and the circulation cells are connected to the outer wall of the fixed box; the peristaltic pump is connected inside the fixed box, is connected with each flow cell tube and is used for transmitting the fluid in different flow cells; the device comprises a pneumatic control valve, a plurality of air tanks and a plurality of air tanks, wherein the pneumatic control valve is used for introducing one of nitrogen or air into each flow cell and is connected with each flow cell pipe; the circuit module is used for transmitting instructions for controlling the flow cell and comprises an upper computer and a circuit board which are connected, wherein the upper computer is connected with the flow cell, and the circuit board is electrically connected with the flow cell.
Further, the flow cell includes: the reaction tank is used for accommodating the to-be-detected product and gas; the fixed seat is connected to the bottom of the reaction tank and is vertically connected to the outer wall of the fixed box so as to support the reaction tank; the heating seat is clamped on the outer wall of the reaction tank and is connected with the fixing seat.
Further, a plurality of fixing clamps are arranged on the inner wall and the outer wall of the fixing box, and each fixing clamp is sleeved with a single pinch valve.
Further, the fixed box is provided with on-off valves on the outer wall, and the number of the on-off valves is at least two.
Further, a regulating valve is arranged in the fixed box and is connected with each switch valve pipe.
Further, be equipped with in the fixed box and hold the chamber, peristaltic pump connects the bottom in holding the chamber, peristaltic pump still includes the buckler, the buckler bolt hold the bottom in chamber.
Further, the flow cell further comprises an oxygen electrode, and the oxygen electrode is inserted into the reaction cell in a plugging manner so as to read the oxygen partial pressure in the reaction cell.
Further, the circuit board is provided with a minimum system board, which comprises: MCU, crystal oscillator circuit, reset circuit, download circuit, nonvolatile memory, control row needle draw forth.
Further, still be equipped with the digit board on the circuit board, the digit board includes: the device comprises a J7-PC interface, an AD conversion circuit, a J11-analog interface, a J8-digital board-analog board communication interface and a J12-temperature sensor interface.
Further, still be equipped with the simulation board on the circuit board, the simulation board includes: the device comprises a J4-analog output interface, a J2-interface, a J3-temperature sensor interface, a J1-pump valve electrode control interface, a J5-relay power supply interface and a J6-analog board chip power supply interface; the J4-analog output interface is electrically connected with the J11-analog interface, and the J8-digital board and analog board communication interface is electrically connected with the analog board.
Further, the oxygen carrying-oxygen releasing capacity relative evaluation device further comprises a shell, the fixed box is arranged in the shell, and the switch valve and the regulating valve penetrate out of the outer wall of the shell.
Further, a shell cover is arranged on the shell, the shell cover is hinged with the shell to rotate, and an opening is formed in the shell cover.
The invention has the beneficial effects that:
1. the computer sends the instruction to the equipment, so that the operation mode that the current device needs to manually control the on-off of each valve body is eliminated, the one-key excitation can be carried out on the instructions based on the heating or stirring of the relevant valve body and the pump body, and the time difference required by the response among all parts of the device during operation is further eliminated.
2. Removing the flow path module, reserving the circuit module and the flow path module, uniformly concentrating valves and other parts required by the flow path module in the flow path module, and enabling the re-planned pipelines and wires to be simple and orderly in layout; and the space which is increased after the flow path module is removed can be used for placing a sample test tube rack, so that the whole equipment is compact in layout and greatly improves the integration level.
Drawings
FIG. 1 is a schematic view of the overall structure of the front side of the present invention when the assembled housing cover is closed;
FIG. 2 is a schematic view of the overall structure of the rear side of the present invention when the assembled housing cover is closed;
FIG. 3 is a schematic view of the rear cross-sectional structure of the assembled cover of the present invention when closed;
FIG. 4 is a schematic view of the overall rear structure of the assembled rear cover of the present invention when opened;
FIG. 5 is a schematic view of the overall structure of the assembled front side of the flow module of the present invention;
FIG. 6 is a schematic cross-sectional view based on FIG. 4;
FIG. 7 is a schematic view of the overall structure of the assembled rear side of the flow module of the present invention;
FIG. 8 is a schematic cross-sectional view based on FIG. 6;
FIG. 9 is a schematic diagram of the piping connections between the various devices;
in the figure, 1, a flow-through module; 11. a fixed box; 111. a fixing clamp; 112. a single pinch valve; 1121. a single clamp tube one valve; 1122. a single-clamp pipe two-valve; 1123. single clamp tube three valves; 1124. single clamp tube four valves; 1125. a single-clamp pipe five valve; 1126. a single clamp pipe six valve; 113. a switch valve; 1131. switching a valve; 1132. switching a second valve; 114. a three-way valve; 115. a negative pressure pipe; 116. a negative pressure secondary pipe; 117. a blow-down pipe; 12. a flow cell; 121. a reaction tank; 1211. reacting a pool; 1212. a second reaction tank; 1213. an input pipe I; 1214. an input pipe II; 122. a fixing seat; 123. a heating seat; 124. an oxygen electrode; 13. a peristaltic pump; 14. a pneumatic control valve; 15. a receiving chamber; 151. a regulating valve; 1511. adjusting a valve; 1512. regulating the two valves; 152. a waterproof cover; 16. a housing; 161. a cover; 162. an opening; 2. a circuit module; 21. a circuit board; 211. a first integrated board; 212. a second integrated board; 22. and a power supply.
Detailed Description
The following detailed description of the embodiments of the present invention will be made more apparent to those skilled in the art from the following detailed description, in which the invention is embodied in several, but not all, embodiments of the invention. The invention may be embodied or applied in other specific forms and features of the following examples and examples may be combined with each other without conflict, all other examples being contemplated by those of ordinary skill in the art without undue burden from the present disclosure, based on the examples of the invention.
The oxygen carrying-releasing capacity relative evaluation device, as shown in fig. 1-9, comprises: the flow module 1 is used for detecting the oxygen partial pressure value of the to-be-detected product and comprises a fixed box 11 and at least two flow cells 12, wherein the flow cells 12 are connected to the outer wall of the fixed box 11; the device comprises a peristaltic pump 13, wherein the peristaltic pump 13 is connected inside a fixed box 11, the peristaltic pump 13 is connected with each flow cell 12 through a pipe, and fluids in different flow cells 12, which can be one or more of buffer solution, to-be-detected product, air and nitrogen, are transmitted through the peristaltic pump 13; comprises a pneumatic control valve 14 for introducing one of nitrogen or air into each flow cell 12, wherein the pneumatic control valve 14 is connected with each flow cell 12 in a pipe manner; the circuit module 2 is used for transmitting the instruction for controlling the flow cell 12, the circuit module 2 comprises an upper computer and a circuit board 21 which are connected, the upper computer is connected with the flow cell 1, and the circuit board 21 is electrically connected with the flow cell 12. The upper computer is a computer provided with N0PPA-2021 software, the upper computer is connected with the circulation module 1 and the upper computer is communicated with the circuit board 21 through serial ports, so that control instructions are sent to the equipment, data fed back by the equipment can be received, the equipment at least comprises a switch valve 113, a circulation cell 12, a peristaltic pump 13, a pneumatic control valve 14 and a regulating valve 151, after the equipment is assembled and connected, one-key control can be performed through the upper computer, so that samples to be tested can pass smoothly among different equipment, the response time difference caused by manual switching or operation when the samples to be tested circulate among two different equipment is solved, and the whole response time of the relative evaluation device is reduced to the greatest extent. The flow cell 12 comprises a reaction cell 121, and the reaction cell 121 is specifically a reaction first cell 1211 in the flow cell 12 located below and a reaction second cell 1212 in the flow cell 12 located above.
1-9, the two reaction tanks 121 are different, the first reaction tank 1211 is used for mixing the fluid input from the second reaction tank 1212, and the mass of the material input from the first reaction tank 1211 to the second reaction tank 1212 is the same before the reaction starts, so that the volume of the first reaction tank 1211 is at least twice that of the second reaction tank 1212.
In a more preferred embodiment, as shown in fig. 9, different devices are connected through hoses, the number of the switch valves 113 can be two, namely, the switch one valve 1131 and the switch two valve 1132, the number of the regulating valves 151 can be two, two ends of each switch valve 113 are respectively connected with one hose, when the switch valve 113 is in an off state, the two hoses at two ends of the switch valve 113 are not communicated with each other, and when the switch valve 113 is in a communicating state, the two hoses at two ends of the switch valve 113 are communicated with each other. One end of the regulating one valve 1511 is connected with the on-off one valve 1131 through a hose, the other end of the regulating one valve 1511 is connected with the normally-closed single-clamp six-valve 1126 through a hose, and the other end of the single-clamp six-valve 1126 is connected with a hose inserted into the bottom of the reaction two-tank 1212, thereby forming a flow path leading into the reaction two-tank 1212 from the outside. Under the working state, the nitrogen tank is connected with the on-off valve 1131 through a hose, then the on-off valve 1131 and the single-clamp pipe six-valve 1126 are opened, so that nitrogen can be introduced into the reaction second tank 1212, the nitrogen participates in the reaction second tank 1212, the flow of the nitrogen is controlled by adjusting the valve 1511, and the input step of the nitrogen in the reaction tank 121 is completed. The sample to be tested in the first reaction cell 1211 is specifically an oxygen carrier, including one or more of red blood cells, hemoglobin, natural oxygen carriers and artificial oxygen carriers, and after fully reacting in the first reaction cell 1211, the sample to be tested enters the second reaction cell 1212 to be mixed with a buffer solution. Before the reaction, the operator fills the buffer solution into the reaction cell 1212, and fully deoxidizes the buffer solution by the nitrogen gas.
In a more preferred embodiment, as shown in fig. 9, the first reaction tank 1211 and the second reaction tank 1212 are connected by a hose, a single-clamp tube four valve 1124 is provided on the hose, the on-off relationship between the first reaction tank 1211 and the second reaction tank 1212 is switched by opening and closing the single-clamp tube four valve 1124, the first reaction tank 1211 is connected with a first input tube 1213, the second reaction tank 1212 is connected with a second input tube 1214, and the first input tube 1213 and the second input tube 1214 are respectively connected with an external container for inputting the sample or buffer into the two reaction tanks 121.
In a more preferred embodiment, as shown in fig. 9, one end of the two-way valve 1512 is connected to the two-way valve 1132 through a hose, the other end of the two-way valve 1512 is connected to the three-way valve 114 through a hose, one of the other ends of the three-way valve 114 is connected to one end of the normally open five-way valve 1125 through a hose, the other end of the other ends of the three-way valve 114 is connected to a hose between the six-way valve 1126 and the one-way valve 1511, and the two-way valve 1132 is connected to the air tank through a hose, so that when the three-way valve 114 and the five-way valve 1125 are disconnected and the six-way valve 1126 is opened, a passage is formed between the air tank and the two-way reaction tank 1212, thereby allowing air to pass through the two-way reaction tank 1212. When the reaction is performed, a proper amount of air is injected into the reaction second tank 1212, so that the to-be-detected sample can be fully saturated with oxygen; after the detection process is finished, excessive air is filled into the second reaction tank 1212, so that residual nitrogen in the second reaction tank 1212 is extruded, and the influence of the residual nitrogen on the next reaction can be prevented. The other end of the single-clamp five-valve 1125 is communicated with the reaction first tank 1211 through a hose, when the three-way valve 114 is communicated with the single-clamp five-valve 1125 and the two regulating valves 1512, a flow path for filling air into the reaction first tank 1211 can be formed by opening the two switching valves 1132, and the air flow rate is regulated by controlling the two regulating valves 1512 so that the air participates in the reaction first tank 1211.
In a more preferred embodiment, as shown in fig. 9, a hose with a pagoda four-way joint at the end is connected to the inlet of the peristaltic pump 13, the diameter of the connecting port of the pagoda four-way joint is 2.4mm, one port of the four-way joint is connected with a negative pressure first pipe 115 for communicating the peristaltic pump 13 with a reaction first tank 1211, the other port of the four-way joint is connected with a negative pressure second pipe 116 for communicating the peristaltic pump 13 with a reaction second tank 1212, a normally closed single-clamp pipe second valve 1122 is arranged on the negative pressure first pipe 115, and a normally closed single-clamp pipe first valve 1121 is arranged on the negative pressure second pipe 116. The peristaltic pump 13 generates negative pressure by compressing or expanding the internal space and delivers the negative pressure to the two flow cells 12, drawing fluid from the air or nitrogen tank into the flow cells 12. The last port of the four-way joint is connected with a drain pipe 117, and the other end of the drain pipe 117 is connected in series with a hose between a single-clamp pipe five valve 1125 and a reaction one tank 1211; in addition, a hose leading to the outside is connected to the outlet of the peristaltic pump 13. According to the above arrangement, when the detection is completed, the single-pinch one valve 1121, the single-pinch two valve 1122, the single-pinch five valve 1125, and the single-pinch six valve 1126 are closed, and the single-pinch three valve 1123 and the single-pinch four valve 1124 are opened, so that the fluid in the second reaction cell 1212 enters the first reaction cell 1211, the fluid in the two reaction cells 121 is mixed, enters the peristaltic pump 13 through the drain pipe 117, and finally is discharged out of the oxygen carrying-oxygen releasing capacity relative evaluation device through the hose leading to the outside.
More preferred embodiments, as shown in fig. 1-9, the flow cell 12 comprises: a reaction cell 121 for accommodating a sample to be measured and a gas; the fixed seat 122 is connected to the bottom of the reaction tank 121, and the fixed seat 122 is vertically connected to the outer wall of the fixed box 11 to support the reaction tank 121; the heating seat 123 is clamped on the outer wall of the reaction tank 121 and is connected with the fixing seat 122. The heating seat 123 is fixed on the front surface of the fixed box 11, the heating rod on the heating seat 123 penetrates through the fixed box 11, the heating seat 123 needs 24V power supply, so that two power supply 22 wires connected with the power supply 22 penetrate through the fixed box 11 and are respectively in GND and 24V, and then the heating seat 123 is controlled by upper computer software to heat or stop heating.
More preferably, as shown in fig. 1-9, a plurality of fixing clips 111 are provided on the inner wall and the outer wall of the fixing case 11, and each fixing clip 111 is sleeved with a single pinch valve 112.
In a more preferred embodiment, as shown in fig. 1-9, the on-off valves 113 are specifically disposed on the outer wall of the fixed box 11, and the number of the on-off valves 113 is at least two, specifically, the on-off valves comprise a first on-off valve 1131 for controlling the nitrogen injection device and a second on-off valve 1132 for controlling the air injection device, and the operation modes of the first on-off valve 1131 and the second on-off valve 1132 comprise manual screwing and upper computer control, so that the automation is realized, and meanwhile, the emergency termination of the fluid input can be performed manually.
In a more preferred embodiment, as shown in fig. 1-9, the adjusting valve 151 is disposed inside the fixed box 11, the adjusting valve 151 specifically includes an adjusting first valve 1511 and an adjusting second valve 1512, the adjusting first valve 1511 is connected with the opening and closing first valve 1131 through a hose for controlling the flow rate of nitrogen gas when inputting, the adjusting second valve 1512 is connected with the opening and closing second valve 1132 through a hose for controlling the flow rate of air when inputting, and the adjusting first valve 1511 and the adjusting second valve 1512 are both controlled by a single key of the upper computer.
In a more preferred embodiment, as shown in fig. 1-9, the holding chamber 15 is provided in the fixed case 11, the peristaltic pump 13 is connected to the bottom of the holding chamber 15, and the peristaltic pump 13 further includes a waterproof cover 152, where the waterproof cover 152 is bolted to the bottom of the holding chamber 15. When the hose inside the fixed tank 11 leaks, the fluid splashes to the bottom of the fixed tank 11 through the hose, and the waterproof cover 152 can effectively prevent the ejected fluid from directly contacting the peristaltic pump 13.
In a more preferred embodiment, as shown in fig. 1-9, the flow cell 12 further includes an oxygen electrode 124, where the oxygen electrode 124 is inserted into the reaction cell 121, and the oxygen electrode 124 is specifically a Clark electrode, to measure the oxygen partial pressure in the sample, which is a very important parameter in the oxygen saturation process and the neutralization process, must be sent to the host computer through the serial port by the core board, so that the oxygen electrode 124 must be connected to the circuit board 21.
In a more preferred embodiment, as shown in fig. 1-9, the circuit board 21 includes a first integrated board 211 and a second integrated board 212, where the first integrated board 211 has a minimum system board thereon, which includes: MCU, crystal oscillator circuit, reset circuit, download circuit, nonvolatile memory and control row needle draw forth. The power supply 22 is connected to the bottom of the accommodating cavity 15, the power supply 22 is electrically connected with the circuit board 21, the power supply 22 is electrically connected with each device, specifically, the input voltage of the power supply 22 is 220V, the model of the power supply 22 can be + -12v+5v, and the type of the power supply 22 is the switch power supply 22. The MCU can perform data processing and sending, the crystal oscillator circuit is used for providing a clock signal for the minimum system, the reset circuit is used for resetting and restarting the system, the downloading circuit is used for downloading a usable program, the nonvolatile memory is used for storing nonvolatile data related to starting, and the control pin header is led out to be connected with the digital board through the DuPont line.
In a more preferred embodiment, as shown in fig. 1-9, the first integrated board 211 is further provided with a digital board, where the digital board includes: the system comprises a J7-PC interface, an AD conversion circuit, a J11-analog interface, a J8-digital board and analog board communication interface and a J12-temperature sensor interface, wherein the digital board is connected with a minimum system board through 144P pins.
In a more preferred embodiment, as shown in fig. 1-9, the second integrated board 212 is provided with an analog board, the digital board is connected to the analog board through a flat cable, and the analog board includes: the device comprises a J4-analog output interface, a J2-interface, a J3-temperature sensor interface, a J1-pump valve electrode control interface, a J5-relay power supply interface and a J6-analog board chip power supply interface; the J4-analog output interface is electrically connected with the J11-analog interface, the J8-digital board on the analog board is connected with the J7-PC interface on the digital board through the DuPont wire, and the analog board is connected with the upper computer through the USB wire.
In a more preferred embodiment, as shown in fig. 1-9, the relative evaluation device for oxygen carrying capacity and oxygen releasing capacity further comprises a housing 16, the fixed box 11 is arranged inside the housing 16, and the on-off valve 113 and the regulating valve 151 penetrate through the outer wall of the housing 16. The shell 16 is provided with a shell cover 161, the shell cover 161 is hinged with the shell 16, the shell cover 161 is provided with an opening 162, and the opening 162 is arranged for the following purposes: the sample input and cleaning operations are performed to the first reaction cell 1211 and the second reaction cell 1212 without opening the cover 161.
After assembly, the device has the following checking and practical operation procedures:
firstly, buffer solution is input into the reaction first pool 1211 through a hose externally connected to the reaction first pool 1211, and at the moment, a corresponding operation instruction sent to the MCU by the upper computer is as follows: the six-valve 1126 is closed (command: PC_CMD_V6_OFF), the four-valve 1124 is closed (command: PC_CMD_V4_OFF), the one-valve 1121 is closed (command: PC_CMD_V1_OFF), the two-valve 1122 is open (command: PC_CMD_V2_ON), the three-valve 1123 is closed (command: PC_CMD_V3_OFF), the five-valve 1125 is closed (command: PC_CMD_V5_OFF), the peristaltic pump 13 is opened (command: PC_CMD_P1_ON), and the negative pressure of the 1# negative pressure tube is established, thereby sucking buffer from the input tube one 1213.
Preheating the reaction one pool 1211, and introducing nitrogen into the reaction one pool 1211, wherein when the nitrogen is introduced into the reaction one pool 1211, the corresponding operation instruction sent by the upper computer to the MCU is as follows: the six-valve 1126 is closed (command: PC_CMD_V6_OFF), the four-valve 1124 is closed (command: PC_CMD_V4_OFF), the one-valve 1121 is closed (command: PC_CMD_V1_OFF), the two-valve 1122 is closed (command: PC_CMD_V2_OFF), the three-valve 1123 is closed (command: PC_CMD_V3_OFF), the five-valve 1125 is open (command: PC_CMD_V5_ON), and the peristaltic pump 13 is closed (command: PC_CMD_P1_OFF).
When preheating, air is introduced into the reaction first pool 1211, and at this time, the corresponding operation instruction sent by the upper computer to the MCU is: pinch valve 6 is closed (command: PC_CMD_V6_OFF), single-pinch four valve 1124 is closed (command: PC_CMD_V4_OFF), single-pinch one valve 1121 is closed (command: PC_CMD_V1_OFF), single-pinch two valve 1122 is open (command: PC_CMD_V2_ON), single-pinch three valve 1123 is closed (command: PC_CMD_V3_OFF), single-pinch five valve 1125 is open (command: PC_CMD_V5_ON), peristaltic pump 13 is closed (command: PC_CMD_P1_OFF).
After the preheating is finished, the reaction first tank 1211 is emptied, and the reaction first tank 1211 and the reaction second tank 1212 are respectively injected with the to-be-detected product, and when the reaction first tank 1211 is emptied, the corresponding operation instruction sent by the upper computer to the MCU is: pinch valve 6 is closed (command: PC_CMD_V6_OFF), single-pinch four valve 1124 is closed (command: PC_CMD_V4_OFF), single-pinch one valve 1121 is closed (command: PC_CMD_V1_OFF), single-pinch two valve 1122 is closed (command: PC_CMD_V2_OFF), single-pinch three valve 1123 is open (command: PC_CMD_V3_ON), single-pinch five valve 1125 is closed (command: PC_CMD_V5_OFF), peristaltic pump 13 is open (command: PC_CMD_P1_ON).
The to-be-detected product is input into the reaction first pool 1211 through the input pipe 1213, and the corresponding operation instruction sent by the upper computer to the MCU is as follows: the six-valve 1126 is closed (instruction: PC_CMD_V6_OFF), the four-valve 1124 is closed (instruction: PC_CMD_V4_OFF), the one-valve 1121 is closed (instruction: PC_CMD_V1_OFF), the two-valve 1122 is open (instruction: PC_CMD_V2_ON), the three-valve 1123 is closed (instruction: PC_CMD_V3_OFF), the five-valve 1125 is closed (instruction: PC_CMD_V5_OFF), the peristaltic pump 13 is opened (instruction: PC_CMD_P1_ON), and the negative pressure is established in the one tube 115, thereby allowing the sample to be tested to be sucked into the reaction cell 1211.
The to-be-detected product is input into the reaction second pool 1212 through the input pipe II 1214, and the corresponding operation instruction sent by the upper computer to the MCU is as follows: the six-valve 1126 is closed (instruction: PC_CMD_V6_OFF), the four-valve 1124 is closed (instruction: PC_CMD_V4_OFF), the one-valve 1121 is opened (instruction: PC_CMD_V1_ON), the two-valve 1122 is closed (instruction: PC_CMD_V2_OFF), the three-valve 1123 is closed (instruction: PC_CMD_V3_OFF), the five-valve 1125 is closed (instruction: PC_CMD_V5_OFF), and the peristaltic pump 13 is opened (instruction: PC_CMD_P1_ON), so that the sample to be measured is sucked into the reaction cell 1212.
After the to-be-detected product is input, air and nitrogen are respectively input into the primary reaction tank 1212 and the secondary reaction tank 1212, the air input into the primary reaction tank 1211 and the nitrogen input into the secondary reaction tank 1212 can be simultaneously performed, and if the air input into the primary reaction tank 1211 and the nitrogen input into the secondary reaction tank 1212 are simultaneously performed, the corresponding operation instruction sent by the upper computer to the MCU is as follows: the six-valve 1126 is open (command: PC_CMD_V6_ON), the four-valve 1124 is closed (command: PC_CMD_V4_OFF), the one-valve 1121 is closed (command: PC_CMD_V1_ON), the two-valve 1122 is closed (command: PC_CMD_V2_OFF), the three-valve 1123 is closed (command: PC_CMD_V3_OFF), the five-valve 1125 is open (command: PC_CMD_V5_ON), and the peristaltic pump 13 is open (command: PC_CMD_P1_ON).
After the above steps are completed, the single-clamp pipe four-valve 1124 is opened, the fluid in the two reaction tanks 121 is mixed in the reaction tank 1211, the mixing effect is observed through the oxygen electrode 124, and after the reaction is completed, the reaction tank 1211 is emptied again, and the detection is completed.
Claims (8)
1. An oxygen carrying-oxygen releasing capacity relative evaluation device, characterized by comprising:
the circulation module is used for detecting the oxygen partial pressure value of the to-be-detected product and comprises a fixed box and at least two circulation cells, and the circulation cells are connected to the outer wall of the fixed box; the peristaltic pump is connected inside the fixed box, is connected with each flow cell tube and is used for transmitting the fluid in different flow cells; the device comprises a pneumatic control valve, a plurality of air tanks and a plurality of air tanks, wherein the pneumatic control valve is used for introducing one of nitrogen or air into each flow cell and is connected with each flow cell pipe;
the circuit module is used for transmitting an instruction for controlling the flow cell, the circuit module comprises an upper computer and a circuit board which are connected, the upper computer is connected with the flow cell, the circuit board is electrically connected with the flow cell, the upper computer is connected with the flow cell and the circuit board through serial lines, and the upper computer is communicated with the equipment through serial ports, so that the control instruction is sent to the equipment and data fed back by the equipment can be received, the equipment at least comprises a switch valve, a flow cell, a peristaltic pump, a pneumatic control valve and a regulating valve, the flow cell comprises a reaction cell, and the reaction cell is a reaction cell in the flow cell below and a reaction cell in the flow cell above; the number of the switch valves is two, namely a switch one valve and a switch two valve respectively, the number of the regulating valves is two, two ends of each switch valve are respectively connected with a hose, one end of each regulating valve is connected with the switch one valve through the hose, the other end of each regulating valve is connected with a normally closed single-clamp pipe six valve through the hose, and the other end of the single-clamp pipe six valve is connected with the hose inserted into the bottom of the reaction two tanks, so that a flow path which is led into the reaction two tanks from the outside is formed;
the first reaction tank is connected with the second reaction tank through a hose, a single clamping pipe four valve is arranged on the hose, the on-off relation between the first reaction tank and the second reaction tank is switched by opening and closing the single clamping pipe four valve, an input pipe I is connected to the first reaction tank, an input pipe II is connected to the second reaction tank, and the input pipe I and the input pipe II are respectively connected with an external container and are used for inputting to-be-detected products or buffer solution into the two reaction tanks;
one end of the regulating two valves is connected with the two switch valves through hoses, the other end of the regulating two valves is connected with one three-way valve through hoses, one of the other ends of the three-way valve is connected with one end of the normally open single-clamp pipe five valve through hoses, the other end of the other ends of the three-way valve is connected with a hose between the single-clamp pipe six valve and the regulating one valve, the two switch valves are connected with an air tank through hoses, the other end of the single-clamp pipe five valve is communicated with the reaction one tank through hoses, the inlet of the peristaltic pump is connected with a hose with a pagoda four-way joint at the tail end, one port of the four-way joint is connected with a negative pressure one pipe which is communicated with the peristaltic pump and the reaction one tank, the other port of the four-way joint is connected with a negative pressure two pipes which are communicated with the peristaltic pump and the reaction two tanks, the negative pressure one pipe is provided with the normally closed single-clamp pipe two valves, and the negative pressure two pipes are provided with normally closed single-clamp pipe one valve.
2. The oxygen carrying-oxygen releasing capacity relative evaluation device according to claim 1, wherein a fixing base is connected to the bottom of the reaction tank, and the fixing base is vertically connected to the outer wall of the fixing box to support the reaction tank; the heating seat is clamped on the outer wall of the reaction tank and is connected with the fixing seat.
3. The oxygen carrying-oxygen releasing capacity relative evaluation device according to claim 1, wherein a plurality of fixing clamps are arranged on the inner wall and the outer wall of the fixing box, and each fixing clamp is sleeved with a single pinch valve.
4. The oxygen carrying-oxygen releasing capacity relative evaluation device according to claim 1, wherein the fixing box is internally provided with a containing cavity, the peristaltic pump is connected to the bottom of the containing cavity, and the peristaltic pump further comprises a waterproof cover, and the waterproof cover is bolted to the bottom of the containing cavity.
5. The oxygen carrying-oxygen releasing capacity relative evaluation device according to claim 2, wherein the flow cell further comprises an oxygen electrode, and the oxygen electrode is inserted into the reaction cell to read the partial pressure of oxygen in the reaction cell.
6. The oxygen carrying-oxygen evolution capacity relative evaluation device according to claim 1, wherein the circuit board is provided with a minimum system board, comprising: MCU, crystal oscillator circuit, reset circuit, download circuit, nonvolatile memory, control row needle draw forth.
7. The oxygen carrying-oxygen releasing capacity relative evaluation device according to claim 6, wherein the circuit board is further provided with a digital board, the digital board comprises: the device comprises a J7-PC interface, an AD conversion circuit, a J11-analog interface, a J8-digital board-analog board communication interface and a J12-temperature sensor interface.
8. The oxygen carrying-oxygen evolution capacity relative evaluation device according to claim 7, wherein the circuit board is further provided with a simulation board, and the simulation board comprises: the device comprises a J4-analog output interface, a J2-interface, a J3-temperature sensor interface, a J1-pump valve electrode control interface, a J5-relay power supply interface and a J6-analog board chip power supply interface; the J4-analog output interface is electrically connected with the J11-analog interface, and the J8-digital board and analog board communication interface is electrically connected with the analog board.
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