CN117269139A - Real-time monitoring system based on Raman spectrum - Google Patents
Real-time monitoring system based on Raman spectrum Download PDFInfo
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- CN117269139A CN117269139A CN202311164486.8A CN202311164486A CN117269139A CN 117269139 A CN117269139 A CN 117269139A CN 202311164486 A CN202311164486 A CN 202311164486A CN 117269139 A CN117269139 A CN 117269139A
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- 238000012544 monitoring process Methods 0.000 title claims abstract description 39
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- 239000007788 liquid Substances 0.000 claims description 53
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- 238000001069 Raman spectroscopy Methods 0.000 claims description 22
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- 239000000523 sample Substances 0.000 claims 27
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- G—PHYSICS
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a real-time monitoring system based on Raman spectrum, which comprises: the equipment interface module is used for controlling disconnection of the spectrometer, setting of parameters and acquisition of data; the user interface module is interacted with a user and comprises data processing operation and display of data processing results; the data processing module comprises spectrum preprocessing, feature quantity extraction and a mathematical model algorithm. According to the invention, chemical components and structures in a sample can be rapidly, accurately and real-time monitored and analyzed through a non-invasive Raman spectrum technology, and compared with a spectrum analysis method based on photon scattering phenomenon caused by molecular vibration and rotation, the Raman spectrum has the characteristics of very abundant chemical information and high specificity, and can be used for carrying out non-destructive analysis on molecular bonds, chemical components and crystal structures in the sample under the condition of no sample treatment.
Description
Technical Field
The invention relates to the technical field of chemical component monitoring, in particular to a real-time monitoring system based on Raman spectrum.
Background
In the fields of scientific research, industrial production, quality control and the like, monitoring chemical components and structural changes of samples in real time is important for understanding material characteristics, optimizing process flows and ensuring product quality.
The conventional real-time monitoring method has some limitations, such as the need of sample treatment or sampling, destructive testing of the sample, slower monitoring speed, etc., and in order to solve these problems, the present application aims to develop a real-time monitoring system, which can rapidly, accurately and real-time monitor and analyze chemical components and structures in the sample by using a non-invasive monitoring technology.
Disclosure of Invention
The invention aims to provide a Raman spectrum-based real-time monitoring system which can rapidly, accurately and real-timely monitor and analyze chemical components and structures in a sample.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a real-time monitoring system based on raman spectroscopy, comprising:
the equipment interface module is used for controlling disconnection of the spectrometer, setting of parameters and acquisition of data;
the user interface module is interacted with a user and comprises data processing operation and display of data processing results;
the data processing module comprises spectrum preprocessing, feature quantity extraction and a mathematical model algorithm.
Preferably, the device interface module comprises a hardware system having:
a laser source for providing a laser beam for exciting the sample to generate raman scattered light;
an optical assembly for manipulating the laser beam and collecting raman scattered light of the sample;
the detector is used for collecting Raman scattered light signals of the sample, is responsible for receiving and measuring the intensity of the scattered light and converting the intensity of the scattered light into an electric signal, and comprises a photodiode and a photomultiplier;
the spectrometer is used for dispersing and recording the raman scattering spectrum of the sample, and is usually composed of a grating for dispersing light of different wavelengths into different angles to obtain spectral information of the sample and a detector for measuring the intensity of the light of different wavelengths.
Preferably, the hardware system further comprises:
the rectangular block is internally provided with a closed rectangular cavity;
the first U-shaped frame is fixedly arranged below the rectangular block and forms an open space with the rectangular block;
the second U-shaped frame is inversely and fixedly arranged in the rectangular cavity, so that the rectangular cavity is divided into two parts, namely a light beam path area and a liquid sample collecting area;
the laser source is arranged on one side of the second U-shaped frame, the first filter lens, the collimating lens and the first reflector are sequentially arranged in a path of the laser source emitting light beams, the detector is arranged on the other side of the second U-shaped frame, the second reflector, the second filter lens and the focusing lens are sequentially arranged in a path of the detector receiving the light beams, and the first reflector and the second reflector are symmetrically arranged and form an included angle of 90 degrees.
Preferably, a sample detection component is arranged on the second U-shaped frame, the sample detection component is located at the middle section position between the first reflecting mirror and the second reflecting mirror, the sample detection component comprises a fixed block fixed on the second U-shaped frame, a circular cavity is formed in the fixed block, a rotatable turning component is arranged in the circular cavity, a detection ring is fixedly connected in the circular cavity, the detection ring is provided with an upper opening and a lower opening, and the liquid sample in the detection ring is in an intermittent flow state along with the rotation of the turning component.
Preferably, the turnover part comprises a rotating ring which is sleeved on the outer ring of the detection ring and is provided with an upper notch and a lower notch, the outer ring of the rotating ring is fixedly connected with two rotating blocks, the two rotating blocks are symmetrically arranged by virtual connecting lines of the upper notch and the lower notch, one end of each rotating block is attached to the inner ring of the circular cavity, the rotating ring is coaxially and fixedly connected with a ring gear, a servo motor is arranged on each fixed block, an output shaft of the servo motor is fixedly connected with a driving gear, the driving gears are mutually meshed with the ring gear, and a glass lens is adopted in a horizontal overlapping area of each fixed block and the detection ring.
Preferably, the top surface of fixed block and the contact of rectangle cavity interior top surface, the perpendicular circular slot has been seted up to the fixed block, the bottom intercommunication circular cavity of circular slot, the top of circular slot upwards continues to extend, run through the top surface of rectangle piece, at the top surface fixed connection pipe of rectangle piece, the hole and the circular slot of pipe are positive to correspond, pipe top threaded connection dome, the pipe outer wall sets up the external screw thread, the dome inner wall sets up the internal thread, through opening the dome, can empty liquid sample into the pipe, and fall into in the circular cavity through the circular slot.
Preferably, the bottom surface fixed connection connecting block of fixed block, the outflow groove that runs through from top to bottom is seted up to the connecting block, the top and the circular cavity intercommunication of outflow groove, set up the part that opens and shuts in the outflow groove for the circulation state of control outflow groove, the part that opens and shuts includes two pneumatic cylinders of fixed mounting in the fixed block bottom surface, the output shaft fixed connection piece that opens and shuts of pneumatic cylinder, the piece that opens and shuts has the big piece that the thickness is less and the thick big piece of thickness, the piece runs through the connecting block and with connecting block sliding seal connection, the big piece is located the outflow inslot portion, when two big pieces contradict each other, then the outflow groove is sealed, the liquid sample in the circular groove cavity can't flow out, the bottom of connecting block extends to the second U shaped frame for outflow groove and liquid sample collection area intercommunication.
Preferably, the bottom surface of the rectangular block is provided with a row hole, the top end of the row hole is communicated with the liquid sample collecting area, the bottom end of the row hole is provided with a blocking component, the blocking component comprises a rotating plate which is rotationally connected with the bottom surface of the rectangular block, the upper side of the rotating plate is fixedly connected with a rubber block, the rubber block is round and has a diameter larger than that of the row hole, the rotating plate is fixedly connected with a driven gear, a straight cylinder is fixedly connected on a first U-shaped frame, a rotating shaft is coaxially arranged in the straight cylinder, the rotating shaft is rotationally connected with the first U-shaped frame, a torsion spring is arranged in the straight cylinder, the torsion spring is sleeved on the periphery of the rotating shaft, two ends of the torsion spring are respectively connected with the straight cylinder and the rotating shaft, the rotating shaft is fixedly sleeved with a driving disc, the outer ring of the driving disc is provided with a tooth groove, so that the driving disc is meshed with the driven gear, and the driving disc is driven by stirring the rubber block to be separated from the bottom end of the row hole, and samples in the liquid sample collecting area can be normally discharged.
A method for using a hardware system in a real-time monitoring system based on Raman spectrum comprises the following steps:
starting a servo motor to drive the rotating ring to rotate, so that the notch and the opening are staggered, one rotating block is positioned right above the rotating ring, opening the round cover, pouring the liquid sample into the round pipe until the liquid sample fills up two sides of the two rotating blocks;
continuously driving the rotating ring to rotate, so that the liquid sample at one side of the two rotating blocks enters the detecting ring from the notch to the opening by utilizing gravity, and at the moment, the light beam emitted by the laser source passes through the liquid sample to excite the sample to generate Raman scattered light;
intermittently rotating the rotating ring to periodically change the flow of the liquid sample in the detecting ring;
starting the hydraulic cylinder to separate the two opening and closing blocks, so that the liquid sample in the circular cavity flows into the outflow groove and is accumulated in the liquid sample collecting area;
the driving disc is stirred to drive the driven gear to rotate, and then the rotating plate is driven to rotate, so that the rubber block is separated from the discharge hole, and then the liquid sample accumulated in the liquid sample collecting area is discharged.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, chemical components and structures in a sample can be rapidly, accurately and real-time monitored and analyzed through a non-invasive Raman spectrum technology, and compared with a spectrum analysis method based on photon scattering phenomenon caused by molecular vibration and rotation, the Raman spectrum has the characteristics of very abundant chemical information and high specificity, and can be used for carrying out non-destructive analysis on molecular bonds, chemical components and crystal structures in the sample under the condition of no sample treatment.
2. According to the invention, the servo motor is adopted to drive the rotating ring to rotate, so that the liquid sample in the detecting ring is intermittently led out and filled, and the liquid sample in the detecting ring is periodically changed, so that the liquid sample is subjected to sampling inspection type monitoring by the monitoring system, and the problem that the liquid sample is difficult to monitor comprehensively due to poor standing fluidity of the liquid sample is avoided.
Drawings
Fig. 1 is a schematic structural diagram of a hardware system in a real-time monitoring system based on raman spectrum according to the present invention;
FIG. 2 is a schematic view of the structure of the rectangular chamber in FIG. 1;
FIG. 3 is a schematic elevational view of the structure of FIG. 2;
fig. 4 is a schematic diagram of the internal structure of a fixed block in a real-time monitoring system hardware system based on raman spectrum according to the present invention;
FIG. 5 is a schematic view of the structure of FIG. 4 from another perspective;
fig. 6 is a schematic structural diagram of a detection ring, a rotary ring, a ring gear and a rotary block in a real-time monitoring system hardware system based on raman spectrum according to the present invention;
fig. 7 is a schematic structural diagram of a connection block and two opening and closing blocks in a real-time monitoring system hardware system based on raman spectrum according to the present invention;
fig. 8 is a schematic structural diagram between a rotating plate and an active disc in a real-time monitoring system hardware system based on raman spectrum according to the present invention;
fig. 9 is a schematic diagram of a sectional structure of an active disc and a cylinder in a hardware system of a real-time monitoring system based on raman spectrum according to the present invention;
fig. 10 is a schematic diagram of each module of a real-time monitoring system based on raman spectrum according to the present invention;
fig. 11 is a schematic flow chart of data processing in a real-time monitoring system based on raman spectrum according to the present invention.
In the figure: 1. rectangular blocks; 2. a rectangular chamber; 3. a first U-shaped frame; 4. a second U-shaped frame; 5. a beam path region; 6. a liquid sample collection area; 7. a laser source; 8. a first filter; 9. a collimator lens; 10. a first mirror; 11. a detector; 12. a second mirror; 13. a second filter; 14. a focusing lens; 15. a fixed block; 16. a circular chamber; 17. a detection ring; 18. an opening; 19. a rotating ring; 20. a notch; 21. a rotating block; 23. a ring gear; 24. a servo motor; 25. a drive gear; 26. a glass lens; 27. a circular groove; 28. a round tube; 29. a dome; 30. a connecting block; 31. an outflow groove; 32. a hydraulic cylinder; 33. an opening and closing block; 34. a small block; 35. large blocks; 36. arranging holes; 37. a rotating plate; 38. a rubber block; 39. a driven gear; 40. a straight cylinder; 41. a rotating shaft; 42. a torsion spring; 43. a driving disk; 44. tooth slots.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and all other embodiments obtained by those skilled in the art without making creative efforts based on the embodiments of the present invention are included in the protection scope of the present invention.
1-11, a Raman spectrum-based real-time monitoring system comprises an equipment interface module, a user interface module and a data processing module, wherein the equipment interface module is responsible for disconnection of a Raman spectrometer, parameter setting and data acquisition; the user interface module is responsible for interaction with a user, comprises data processing related operations and display of data processing results; the data processing module comprises spectrum preprocessing, feature quantity extraction and a mathematical model algorithm;
the device interface module includes a hardware system having:
a laser source 7 for providing a laser beam for exciting the sample to generate raman scattered light;
an optical assembly for manipulating the laser beam and collecting raman scattered light of the sample;
the detector 11 is used for collecting raman scattered light signals of a sample and is responsible for receiving and measuring the intensity of the scattered light and converting the intensity of the scattered light into an electric signal, and the detector 11 comprises a photodiode and a photomultiplier;
the spectrometer is used for dispersing and recording the raman scattering spectrum of the sample, and is usually composed of a grating for dispersing light of different wavelengths into different angles to obtain spectral information of the sample, and a detector 11 for measuring the intensity of the light of different wavelengths.
The data processing flow is as follows:
the first step: data acquisition, namely acquiring Raman scattering spectrum data of a sample through a detector 11;
and a second step of: the spectrum preprocessing, which is to preprocess the collected original spectrum data, comprises the steps of background correction, denoising, signal enhancement and the like, so as to reduce interference and improve signal quality;
and a third step of: feature extraction, extracting useful features such as peak position, peak intensity, peak shape and the like from the pretreated spectrum data;
fourth step: the data dimension reduction is carried out, and dimension reduction processing is carried out on the extracted feature quantity data, so that the data dimension and redundant information are reduced, and the efficiency and accuracy of subsequent analysis are improved;
fifth step: quantitatively analyzing, namely processing the feature quantity data after dimension reduction by using the established quantitative analysis model to obtain the concentration or content of the target component in the sample;
establishment of a quantitative analysis model:
the first step: preparation of a sample dataset, collecting a set of samples of known composition and concentration, and collecting their raman spectral data as a training set.
And a second step of: feature selection, selecting the appropriate features from the spectral data of the training set, which should be correlated to the concentration of the compound or component of interest.
And a third step of: training a model, and establishing a quantitative analysis model according to the characteristics and the known concentration of the training set by using a machine learning or statistical method.
Fourth step: model optimization, optimizing and parameter adjusting are carried out on the established model so as to improve the accuracy and stability of prediction.
Fifth step: model validation, the model is validated using another set of independent sample data sets, and the predictive performance of the model is evaluated.
Sixth step: and (3) applying the model to a Raman real-time monitoring system to realize quantitative analysis of chemical components or molecular structures of unknown samples.
System algorithm:
data preprocessing algorithm: normalization algorithms (min-max normalization, 0 mean normalization), filtering denoising algorithms (sliding mean filtering, S-G filtering), baseline correction algorithms (local extremum median method, polynomial fitting method), deglittering algorithms (median filtering method, hampel filtering method).
Feature quantity extraction algorithm: amplitude method, first derivative method, second derivative method, continuous wavelet transform method and double-scale correlation algorithm;
spectral discrimination algorithm: a hybrid method based on distance measure, similarity function, matching spectrum peak, correlation coefficient and spectrum peak matching;
mathematical model building algorithm: unitary linear regression (SLR), multiple Linear Regression (MLR), principal Component Analysis (PCA), partial Least Squares (PLS), deep learning (TensorFlow), support Vector Machine (SVM).
Through data processing and system algorithm, can extract useful information from complicated Raman spectrum data, carry out real-time analysis and monitoring, realize the quick discernment and quantitative analysis to the target material, can regard data and analysis result that monitor as feedback control's important input, realize the real-time regulation and control to the monitored object, through the integration with feedback control system, can realize the accurate control and the regulation to the target material, in order to satisfy specific requirement or realize optimizing effect, real-time monitoring system based on Raman spectrum can be applied to many fields such as food safety, environmental monitoring, medicine quality control. Whether the real-time quality monitoring in industrial production or the safety detection in daily life, the system has wide application prospect and provides real-time monitoring and early warning functions. The system can monitor the concentration, the composition and the change trend of the target substance in real time, and timely gives out early warning when abnormal conditions are found, so that timely decision support and intervention opportunities are provided for users, and risks and losses are reduced.
The hardware system further comprises:
the rectangular block 1 is internally provided with a closed rectangular cavity 2;
the first U-shaped frame 3 is fixedly arranged below the rectangular block 1 and forms an open space with the rectangular block 1;
the second U-shaped frame 4 is inversely and fixedly arranged inside the rectangular chamber 2, so that the rectangular chamber 2 is divided into two parts, namely a light beam path area 5 and a liquid sample collecting area 6;
the laser source 7 is arranged at one side of the second U-shaped frame 4, the first filter 8, the collimating mirror 9 and the first reflecting mirror 10 are sequentially arranged in a path of emitting light beams from the laser source 7, the detector 11 is arranged at the other side of the second U-shaped frame 4, the second reflecting mirror 12, the second filter 13 and the focusing lens 14 are sequentially arranged in a path of receiving light beams from the detector 11, the first reflecting mirror 10 and the second reflecting mirror 12 are symmetrically arranged and form an included angle of 90 degrees with each other, the laser source 7 emits light beams vertically from bottom to top, and the detector 11 receives vertical light beams from right above;
a sample detecting part is disposed on the second U-shaped frame 4, and the sample detecting part is located at a middle position between the first reflecting mirror 10 and the second reflecting mirror 12, and includes:
the fixed block 15 is fixed on the second U-shaped frame 4, a circular cavity 16 is formed in the fixed block 15, a rotatable turning component is arranged in the circular cavity 16, a detection ring 17 is fixedly connected in the circular cavity 16, the detection ring 17 is provided with an upper opening and a lower opening 18, and the liquid sample in the detection ring 17 is in an intermittent flow state along with the rotation of the turning component;
the turnover part comprises a rotary ring 19 which is sleeved on the outer ring of the detection ring 17 and is matched with the outer ring of the detection ring, the rotary ring 19 is provided with an upper notch 20 and a lower notch 20, the outer ring of the rotary ring 19 is fixedly connected with two rotary blocks 21, the two rotary blocks 21 are symmetrically arranged by virtual connecting lines of the upper notch 20 and the lower notch 20, one end of each rotary block 21 is attached to the inner ring of the circular cavity 16, the rotary ring 19 is fixedly connected with a ring gear 23 in a coaxial mode, a servo motor 24 is arranged on the fixed block 15, an output shaft of the servo motor 24 is fixedly connected with a driving gear 25, the driving gear 25 is in meshed connection with the ring gear 23 to realize driving rotation and stopping rotation of the rotary ring 19, and a glass lens 26 is adopted in a horizontal overlapping area of the fixed block 15 and the detection ring 17 so that light beams pass through a liquid sample;
the top surface of the fixed block 15 is contacted with the inner top surface of the rectangular cavity 2, the fixed block 15 is provided with a vertical round groove 27, the bottom end of the round groove 27 is communicated with the round cavity 16, the top end of the round groove 27 continues to extend upwards and penetrates through the top surface of the rectangular block 1, a round pipe 28 is fixedly connected to the top surface of the rectangular block 1, an inner hole of the round pipe 28 corresponds to the round groove 27, the top end of the round pipe 28 is in threaded connection with a round cover 29, an external thread is arranged on the outer wall of the round pipe 28, an internal thread is arranged on the inner wall of the round cover 29, and a liquid sample can be poured into the round pipe 28 by opening the round cover 29 and falls into the round cavity 16 through the round groove 27;
the bottom surface of the fixed block 15 is fixedly connected with the connecting block 30, the connecting block 30 is provided with an outflow groove 31 penetrating up and down, the top end of the outflow groove 31 is communicated with the circular cavity 16, an opening and closing component is arranged in the outflow groove 31 and used for controlling the circulation state of the outflow groove 31, the opening and closing component comprises two hydraulic cylinders 32 fixedly arranged on the bottom surface of the fixed block 15, an output shaft of each hydraulic cylinder 32 is fixedly connected with an opening and closing block 33, each opening and closing block 33 is provided with a small block 34 with smaller thickness and a large block 35 with thicker thickness, the small blocks 34 penetrate through the connecting block 30 and are in sliding sealing connection with the connecting block 30, the large blocks 35 are positioned in the outflow groove 31, when the two large blocks 35 are mutually abutted, the outflow groove 31 is closed, a liquid sample in the cavity of the circular groove 27 cannot flow out, and the bottom end of the connecting block 30 extends to the second U-shaped frame 4, so that the outflow groove 31 is communicated with the sample collecting area 6;
the bottom surface of the rectangular block 1 is provided with a row hole 36, the top end of the row hole 36 is communicated with the liquid sample collecting area 6, the bottom end of the row hole 36 is provided with a blocking component, the blocking component comprises a rotating plate 37 which is rotationally connected with the bottom surface of the rectangular block 1, the upper side of the rotating plate 37 is fixedly connected with a rubber block 38, the rubber block 38 is round and has a diameter larger than that of the row hole 36, the rotating plate 37 is fixedly connected with a driven gear 39, a straight cylinder 40 is fixedly connected onto the first U-shaped frame 3, a rotating shaft 41 is coaxially arranged in the straight cylinder 40, the rotating shaft 41 is rotationally connected with the first U-shaped frame 3, a torsion spring 42 is arranged in the straight cylinder 40, the torsion spring 42 is sleeved on the periphery of the rotating shaft 41, two ends of the torsion spring 42 are respectively connected with the straight cylinder 40 and the rotating shaft 41, the rotating shaft 41 is fixedly sleeved with a driving disc 43, a tooth groove 44 is arranged on the outer ring of the driving disc 43, so that the driving disc 43 is meshed with the driven gear 39, the driving disc 38 is driven by stirring the driving disc 43 to be separated from the bottom end of the row hole 36, and samples in the liquid sample collecting area 6 can be normally discharged.
Working principle:
starting a servo motor 24 to drive the rotary ring 19 to rotate, so that the notch 20 and the opening 18 are staggered, one rotary block 21 is positioned right above, opening a round cover 29, pouring the liquid sample into the round pipe 28 until the liquid sample fills up two sides of the two rotary blocks 21;
continuously driving the rotating ring 19 to rotate, so that the liquid sample at one side of the two rotating blocks 21 enters the detecting ring 17 from the notch 20 to the opening 18 by utilizing gravity, and at the moment, the light beam emitted by the laser source 7 passes through the liquid sample to excite the sample to generate Raman scattered light;
intermittently rotating the rotating ring 19 so that the liquid sample in the detection ring 17 periodically flows;
the hydraulic cylinder 32 is started to separate the two opening and closing blocks 33 from each other, so that the liquid sample in the circular chamber 16 flows into the outflow groove 31 and is accumulated in the liquid sample collecting area 6;
the driving disc 43 is shifted to drive the driven gear 39 to rotate, and the rotating plate 37 is driven to rotate, so that the rubber block 38 is separated from the discharge hole 36, and the liquid sample accumulated in the liquid sample collecting area 6 is discharged.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (9)
1. A real-time monitoring system based on raman spectroscopy, comprising:
the equipment interface module is used for controlling disconnection of the spectrometer, setting of parameters and acquisition of data;
the user interface module is interacted with a user and comprises data processing operation and display of data processing results;
the data processing module comprises spectrum preprocessing, feature quantity extraction and a mathematical model algorithm.
2. The real-time raman spectrum based monitoring system according to claim 1, wherein the device interface module comprises a hardware system having:
a laser source (7) for providing a laser beam for exciting the sample to produce raman scattered light;
an optical assembly for manipulating the laser beam and collecting raman scattered light of the sample;
the detector (11) is used for collecting Raman scattered light signals of a sample and is responsible for receiving and measuring the intensity of the scattered light and converting the intensity of the scattered light into an electric signal, and the detector (11) comprises a photodiode and a photomultiplier;
the spectrometer is used for dispersing and recording the Raman scattering spectrum of the sample, and is usually composed of a grating and a detector (11), wherein the grating is used for dispersing light with different wavelengths into different angles so as to obtain spectrum information of the sample, and the detector (11) measures the intensity of the light with different wavelengths.
3. The real-time raman spectrum based monitoring system according to claim 2, wherein the hardware system further comprises:
the rectangular block (1) is internally provided with a closed rectangular cavity (2);
the first U-shaped frame (3) is fixedly arranged below the rectangular block (1) and forms an open space with the rectangular block (1);
the second U-shaped frame (4) is inversely and fixedly arranged inside the rectangular cavity (2) so that the rectangular cavity (2) is divided into two parts, namely a light beam path area (5) and a liquid sample collecting area (6);
the laser source (7) is arranged on one side of the second U-shaped frame (4), the first filter lens (8), the collimating lens (9) and the first reflecting lens (10) are sequentially arranged in a path of light beams emitted by the laser source (7), the detector (11) is arranged on the other side of the second U-shaped frame (4), the second reflecting lens (12), the second filter lens (13) and the focusing lens (14) are sequentially arranged in a path of light beams received by the detector (11), and the first reflecting lens (10) and the second reflecting lens (12) are symmetrically arranged and form an included angle of 90 degrees.
4. A real-time monitoring system based on raman spectroscopy according to claim 3, characterized in that a detection sample part is arranged on the second U-shaped frame (4), the detection sample part is positioned at a middle section between the first reflecting mirror (10) and the second reflecting mirror (12), the detection sample part comprises a fixed block (15) fixed on the second U-shaped frame (4), a circular chamber (16) is arranged in the fixed block (15), a rotatable turning part is arranged in the circular chamber (16), a detection ring (17) is fixedly connected in the circular chamber (16), the detection ring (17) is provided with an upper opening and a lower opening (18), and the liquid sample in the detection ring (17) is in an intermittent flow state along with the rotation of the turning part.
5. The real-time monitoring system based on Raman spectrum according to claim 4, wherein the turnover part comprises a rotating ring (19) sleeved on the outer ring of the detection ring (17), the rotating ring (19) is provided with an upper notch (20) and a lower notch (20), the outer ring of the rotating ring (19) is fixedly connected with two rotating blocks (21), virtual connecting lines of the upper notch (20) and the lower notch (20) of the two rotating blocks (21) are symmetrically arranged, one end of each rotating block (21) is attached to the inner ring of the circular chamber (16), the rotating ring (19) is fixedly connected with a ring gear (23) in a coaxial manner, a servo motor (24) is installed on a fixed block (15), an output shaft of the servo motor (24) is fixedly connected with a driving gear (25), the driving gear (25) is connected with the ring gear (23) in a meshed manner, and a glass lens (26) is adopted in a horizontal overlapping area of the fixed block (15) and the detection ring (17).
6. The real-time monitoring system based on Raman spectrum according to claim 5, wherein the top surface of the fixed block (15) is in contact with the inner top surface of the rectangular chamber (2), the fixed block (15) is provided with a vertical round groove (27), the bottom end of the round groove (27) is communicated with the round chamber (16), the top end of the round groove (27) continues to extend upwards, the top surface of the rectangular block (1) is penetrated, the round pipe (28) is fixedly connected to the top surface of the rectangular block (1), the inner hole of the round pipe (28) corresponds to the round groove (27), the top end of the round pipe (28) is in threaded connection with the round cover (29), the outer wall of the round pipe (28) is provided with external threads, the inner wall of the round cover (29) is provided with internal threads, and a liquid sample can be poured into the round pipe (28) by opening the round cover (29), and falls into the round chamber (16) through the round groove (27).
7. The real-time monitoring system based on raman spectrum according to claim 6, wherein the bottom surface of the fixed block (15) is fixedly connected with the connecting block (30), the connecting block (30) is provided with an outflow groove (31) penetrating up and down, the top end of the outflow groove (31) is communicated with the circular chamber (16), an opening and closing component is arranged in the outflow groove (31) and used for controlling the circulation state of the outflow groove (31), the opening and closing component comprises two hydraulic cylinders (32) fixedly installed on the bottom surface of the fixed block (15), the output shaft of the hydraulic cylinders (32) is fixedly connected with the opening and closing block (33), the opening and closing block (33) is provided with a small block (34) with smaller thickness and a large block (35) with thicker thickness, the small block (34) penetrates through the connecting block (30) and is in sliding sealing connection with the connecting block (30), the large block (35) is located inside the outflow groove (31), when the two large blocks (35) are mutually contradicted, the outflow groove (31) is closed, a liquid sample in the circular groove (27) cannot flow out, and the bottom end of the connecting block (30) extends to the second U-shaped outflow frame (4) so that the small block (31) is communicated with the sample collecting area (6).
8. The real-time monitoring system based on Raman spectrum according to claim 7, wherein a row hole (36) is formed in the bottom surface of the rectangular block (1), the top end of the row hole (36) is communicated with the liquid sample collecting area (6), a blocking part is arranged at the bottom end of the row hole (36), the blocking part comprises a rotating plate (37) rotationally connected with the bottom surface of the rectangular block (1), a rubber block (38) is fixedly connected to the upper side of the rotating plate (37), the rubber block (38) is circular, the diameter of the rubber block (38) is larger than that of the row hole (36), the rotating plate (37) is fixedly connected with a driven gear (39), a straight cylinder (40) is fixedly connected to the first U-shaped frame (3), a rotating shaft (41) is arranged in the straight cylinder (40) coaxially, the rotating shaft (41) is rotationally connected with the first U-shaped frame (3), a torsion spring (42) is arranged in the straight cylinder (40), the torsion spring (42) is sleeved on the periphery of the rotating shaft (41), two ends of the torsion spring (42) are respectively connected with the straight cylinder (40) and the rotating shaft (41), the rotating shaft (41) is fixedly sleeved with a driving disc (43), the driving disc (43) is fixedly connected with a driven gear (39), the driving disc (44) is fixedly connected with the driven gear (43), the driven disc is meshed with the driven gear (38), the driven disc (38) through the driving gear (38), the sample in the liquid sample collection area (6) can be normally discharged.
9. The method for using a hardware system in a real-time monitoring system based on raman spectrum according to claim 8, comprising the steps of:
starting a servo motor (24) to drive a rotating ring (19) to rotate, so that a notch (20) and an opening (18) are staggered, one rotating block (21) is positioned right above, opening a round cover (29), pouring a liquid sample into a round pipe (28) until the liquid sample fills two sides of the two rotating blocks (21);
continuously driving the rotating ring (19) to rotate, so that the liquid sample at one side of the two rotating blocks (21) enters the detecting ring (17) from the notch (20) to the opening (18) by utilizing gravity, and at the moment, the light beam emitted by the laser source (7) passes through the liquid sample to excite the sample to generate Raman scattered light;
intermittently rotating the rotating ring (19) such that the periodic flow of the liquid sample within the probe ring (17) is varied;
starting the hydraulic cylinder (32) to separate the two opening and closing blocks (33) from each other, so that the liquid sample in the circular chamber (16) flows into the outflow groove (31) and is accumulated in the liquid sample collecting area (6);
the driving disc (43) is shifted to drive the driven gear (39) to rotate, and then the rotating plate (37) is driven to rotate, so that the rubber block (38) is separated from the discharge hole (36), and the liquid sample accumulated in the liquid sample collecting area (6) is discharged.
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| CN202311164486.8A CN117269139A (en) | 2023-09-11 | 2023-09-11 | Real-time monitoring system based on Raman spectrum |
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