CN117264729A - PCR tube cover and application - Google Patents

PCR tube cover and application Download PDF

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Publication number
CN117264729A
CN117264729A CN202210685345.XA CN202210685345A CN117264729A CN 117264729 A CN117264729 A CN 117264729A CN 202210685345 A CN202210685345 A CN 202210685345A CN 117264729 A CN117264729 A CN 117264729A
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tube
pcr
cover
liquid channel
liquid
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谭若颖
从青
杨谷烨莉
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BIOVUE TECHNOLOGY Ltd
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BIOVUE TECHNOLOGY Ltd
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Priority to CN202210685345.XA priority Critical patent/CN117264729A/en
Publication of CN117264729A publication Critical patent/CN117264729A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The application discloses a PCR tube cover and application thereof, wherein the PCR tube cover comprises a first tube cover and a second tube cover; a liquid channel is arranged in the first pipe cover, and two ends of the liquid channel can be opened or closed; the liquid channel is provided with an opening for communicating the liquid channel with the PCR tube; the second pipe cover is provided with a first vent pipe and a second vent pipe; the inner section of the tube cover of the first vent tube extends to the bottom of the second PCR tube; the opening of the inner section of the tube cover of the second ventilation tube is positioned at the top of the second PCR tube; the openings of the outer sections of the tube covers of the first ventilation tube and the second ventilation tube can be opened or closed; the outer section of the tube cover of the second vent tube can be communicated with one end of the liquid channel in an open state. By adopting the PCR tube cover, the transfer of liquid is realized under the condition that the tube cover is not required to be opened, and the pollution caused by the liquid in the transfer process is reduced or avoided.

Description

PCR tube cover and application
Technical Field
The application belongs to the technical field of PCR devices, and particularly relates to a PCR tube cover and application.
Background
The PCR (Polymerase Chain Reaction ) technology has become an important role in genetic disease screening, pathogen detection, tumor identification and other aspects because of the characteristics of strong specificity, high sensitivity, good repeatability and the like, and particularly the quantitative PCR technology (qPCR) with higher sensitivity and specificity.
To detect low copy templates, such as several copies of a pathogen in a body fluid, the pathogen nucleic acid template is often amplified by a pre-amplification technique, and the pre-amplified product is transferred to a new PCR tube for detection or identification by qPCR. On the one hand, the preamplified product is easy to generate aerosol in the transfer process, so that the accuracy of qPCR is more seriously influenced, the environmental pollution of a detection chamber can be caused, and serious loss is caused; on the other hand, qPCR techniques are highly sensitive and often suffer from contamination with trace amounts of template in the aerosol in the environment during transfer of the pre-amplified product, resulting in false positives.
For this reason, a need exists for a PCR device that reduces or avoids aerosol contamination from the pre-amplification product transfer process.
Disclosure of Invention
The application aims to provide a PCR tube cover and application thereof, which can reduce or avoid aerosol pollution caused by a pre-amplification product transferring process.
The application provides the following technical scheme for solving the technical problems:
the first aspect of the present application provides a PCR tube cap comprising a first tube cap and a second tube cap;
the first pipe cover is provided with at least one annular bulge for closing the first PCR pipe, the first pipe cover is internally provided with a liquid channel penetrating through the first pipe cover, and two ends of the liquid channel can be opened or closed; the liquid channel is provided with an opening at the position in the annular bulge and is used for communicating the liquid channel with the first PCR tube;
the second pipe cover is provided with a first vent pipe and a second vent pipe; the inner section of the tube cover of the first vent tube extends downwards, and when the second tube cover seals the second PCR tube, the opening of the inner section of the tube cover of the first vent tube is positioned at the bottom of the second PCR tube; the opening of the inner section of the tube cover of the second ventilation tube is positioned at the top of the second PCR tube; the openings of the outer sections of the tube covers of the first ventilation tube and the second ventilation tube can be opened or closed;
the opening of the outer section of the tube cover of the second vent tube is communicated with one end of the liquid channel in an open state.
A second aspect of the present application provides a method of transferring a liquid using the PCR tube cap of the first aspect of the present application, comprising:
filling the liquid into a second PCR tube, sealing the first PCR tube by a first tube cover, and sealing the second PCR tube by a second tube cover;
opening the outer section openings of the tube covers of the first ventilation tube and the second ventilation tube;
opening two ends of a liquid channel of the first tube cover, and communicating an opening of the outer section of the tube of the second vent tube with one end of the liquid channel;
inverting the second PCR tube, introducing gas into the second PCR tube through the first vent tube, and pressing the liquid in the second PCR tube into the liquid channel;
separating the outer section of the tube cover of the second vent tube from the liquid channel, and closing the two ends of the liquid channel;
the liquid in the liquid channel was allowed to enter the first PCR tube by centrifugation.
According to the PCR tube cover, the second vent pipe on the second tube cover is communicated with the liquid channel on the first tube cover, so that liquid transfer is realized without opening the tube cover, and pollution caused by liquid in the transfer process, such as aerosol pollution caused by the qPCR pre-amplification product transfer process, is reduced or avoided.
Drawings
FIG. 1 is a schematic view of a structure in which a liquid passage of a first tube cap communicates with a second vent tube of a second tube cap;
FIG. 2 is a top view of the first tube cap with the liquid passage in communication with the second vent tube of the second tube cap;
FIG. 3 is a schematic view of the structure of the second tube cap;
FIG. 4 is a schematic diagram of the structure of the PCR tube cap and PCR tube connection of the present application;
FIG. 5 is a schematic view showing the appearance of a second tube cover and a second PCR tube of the present application;
FIG. 6 is a schematic diagram of the overall inversion of the PCR tube cap and PCR tube of the present application;
fig. 7 is a schematic structural view of the first tube cap and eight-way tube connection of the present application.
Detailed Description
The terms and descriptions used herein are merely for the purpose of describing particular embodiments and are not intended to limit the application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Furthermore, unless the context requires otherwise, singular terms shall include the plural and plural terms shall include the singular.
Definition of the definition
As used herein, the terms "a" and "an" and "the" and similar referents refer to the singular and the plural, unless the context clearly dictates otherwise.
As used herein, the terms "about" and "similar to" refer to an acceptable error range for a particular value as determined by one of ordinary skill in the art, which may depend in part on the manner in which the value is measured or determined, or on the limitations of the measurement system.
A first aspect of the present application provides a PCR tube cap, as shown in fig. 1-4, comprising a first tube cap 2 and a second tube cap 3;
the first tube cover 2 is provided with at least one annular bulge 14 for closing the first PCR tube, the first tube cover 2 is internally provided with a liquid channel 12 penetrating through the first tube cover 2, and two ends of the liquid channel 12 can be opened or closed; the liquid channel 12 is provided with an opening 13 at a position in the annular bulge 14 for communicating the liquid channel 12 with the first PCR tube 4;
a first ventilation pipe 10 and a second ventilation pipe 11 are arranged on the second pipe cover 3; the inner pipe cover section of the first ventilation pipe 10 extends downwards, and when the second pipe cover 3 seals the second PCR pipe 5, the inner pipe cover section opening of the first ventilation pipe 10 is positioned at the bottom of the second PCR pipe 5; the opening of the inner section of the tube cover of the second ventilation tube 11 is positioned at the top of the second PCR tube 5; the opening of the pipe cover outer section 7 of the first ventilation pipe and the opening of the pipe cover outer section 6 of the second ventilation pipe can be opened or closed;
the opening of the cap outer section 6 of the second vent tube is communicable with one end of the liquid passage 12 in an open state.
In some embodiments, the annular projection 14 is sized to match the caliber at a PCR nozzle as is conventional in the art, such that the annular projection 14 is inserted into the PCR tube, thereby effecting closure of the first PCR tube; the annular protrusion may be provided according to conventional manners in the art, and is not limited herein. In some embodiments, the annular protrusion 14 may be plural and may be used to close plural PCR tubes, where the first PCR tube is the plural PCR tubes; in some embodiments, the first PCR tube may be a standard eight-way tube in the art, and the annular protrusion 14 is disposed on the first tube cover at a position matching the eight-way tube, so as to seal eight lumens of the eight-way tube; an opening 13 is provided inside each annular projection 14 for communicating the liquid channel with the lumen.
In some embodiments, the two ends of the liquid channel 12 are opened and closed by a detachable plug or cover 1; when the liquid in the second PCR tube is required to be transferred into the first PCR tube, a plug or a cover at two ends of the liquid channel 12 is opened, one end of the plug or the cover is communicated with the second vent tube, and the other end of the plug or the cover is communicated with the atmosphere, so that the liquid in the second PCR tube enters the liquid channel; after all the liquid enters the liquid channel, the plugs or covers at the two ends of the liquid channel 12 are closed, so that the first PCR tube is sealed.
In some embodiments, the liquid channels have the same inner diameter at different locations, and when the first PCR tube comprises a plurality of PCR tubes (e.g., eight-piece tubes), the liquid in the liquid channel 12 can be evenly divided into the different PCR tubes.
In some embodiments, the opening 13 in the annular projection 14 may be provided as a longitudinal conduit with one end in communication with the liquid passage and one end open.
In some embodiments, the opening of the inner section of the tube cover of the first ventilation tube is located at the bottom of the second PCR tube, which is understood that when the second PCR tube 5 is closed by the second tube cover 3, the opening of the inner section of the tube cover of the first ventilation tube is located at a distance of not more than 1/3, 1/4, 1/5 or 1/6 of the height of the second PCR tube, which is understood that when the liquid in the second PCR tube needs to be moved into the first PCR tube, the second PCR tube needs to be inverted, and at this time, the opening of the inner section of the tube cover of the first ventilation tube should be located above the liquid surface.
In some embodiments, the first vent tube 10 may also be used to replenish the second PCR tube with liquid without uncapping. Illustratively, with the second PCR tube in place, the liquid to be replenished is injected into the second PCR tube through the syringe from the opening of the tube cap outer section 7 of the first vent tube.
In some embodiments, the opening of the tube cap inner section of the second vent tube is located at the top of the second PCR tube, preferably the opening of the tube cap inner section of the second vent tube is located within the second tube cap; it will be appreciated that when the second PCR tube is inverted, the opening of the inner section of the tube cap of the second vent tube should be below the liquid level; the shorter the inner section of the tube cover of the second ventilation tube is, the closer the opening of the inner section of the tube cover is to the top of the second tube cover, and the more favorable the complete transfer of liquid is.
In some embodiments, a syringe may be used to pump gas into the second PCR tube through the opening of the outer section of the tube cover of the first vent tube, to transfer liquid in the second PCR tube; in some embodiments, the outer diameter of the outer section of the first vent tube cap may be matched with the inner diameter of the syringe barrel head, i.e., the outer section of the first vent tube cap can be inserted into the syringe barrel head, thereby achieving a sealed connection between the two. In other embodiments, the inner diameter of the opening of the outer section of the first vent pipe cover is matched with the outer diameter of the syringe needle, and the syringe needle can be inserted into the opening of the outer section of the first vent pipe cover, so that the two can be connected in a sealing way.
In some embodiments, the opening of the tube cover outer section 7 of the first vent tube and the opening of the tube cover outer section 6 of the second vent tube may be opened and closed by a removable plug or cap; in other embodiments, the opening of the outer tube cover section 7 of the first ventilation tube and the opening of the outer tube cover section 6 of the second ventilation tube are sealed when leaving the factory, and the opening can be realized by cutting or punching in use.
In some embodiments, the tube cap outer section of the second vent tube is perpendicular to the axis of the second PCR tube when the second tube cap closes the second PCR tube. It can be understood that when the first PCR tube and the second PCR tube are in the upright state, the liquid channel in the first tube cover is in the horizontal state, and at this time, the outer section of the tube cover of the second ventilation tube is also in the horizontal state, so that the connection of the two is facilitated.
In some embodiments, a push-pull protrusion 8 is further provided on the second tube cover to facilitate push-in and pull-out of the second tube cover onto the second PCR tube.
In some embodiments, the bottom of the second tube cover is provided with a plug port 9 adapted to the PCR tube, and the plug port is used for being inserted into the PCR tube to realize the sealing of the PCR tube. The plug is a conventional arrangement in the art, and the application is not limited herein.
In some embodiments, one end of the liquid channel 12 can be connected in a sealing manner to the outer tube cover section 6 of the second ventilation tube 11 in the open state.
In some embodiments, the inner diameter of one end of the liquid channel 12 is equal to the outer diameter of the outer tube cover section 6 of the second ventilation tube, and at this time, the second ventilation tube cover section 6 may be inserted into one end of the liquid channel 12, so as to realize a sealing connection between the two.
In some embodiments, one end of the liquid channel 12 is detachably connected to the outer tube cover section 6 of the second vent tube.
In some embodiments, when the first PCR tube is an eight-tube, the first tube cover is uniformly provided with eight annular protrusions 14, and openings 13 are formed in positions of the liquid channels 12 in the annular protrusions 14, so as to be used for communicating the liquid channels with eight lumens of the eight-tube.
In some embodiments, the liquid channel 12 has a volume of 20-100 μl. It should be noted that the volume of the liquid channel in the present application may have different designs to meet the requirement of transferring different volumes of liquid, and preferably, the total volume of the liquid channel is substantially equal to or slightly smaller than the volume of the liquid in the second PCR tube.
In some embodiments, the total volume of the liquid channel can be controlled by controlling the inner diameter of the designed liquid channel, and controlling the volume of liquid dispensed into each lumen of the first PCR tube, e.g., in some embodiments, by using a smaller inner diameter when the lengths are the same, the liquid channel has a smaller volume, and the liquid dispensed into each lumen also has a smaller volume; in other embodiments, the fluid passage may have a larger volume by using a larger inner diameter, and the fluid dispensed into each lumen may have a larger volume.
The material of the PCR tube cover in the application can be the same as that of the PCR tube, and the application is not limited herein; when the PCR tube cover is matched with the PCR tube to perform a specific reaction, the PCR tube cover can be subjected to the same pretreatment as the PCR tube, and the application is not limited herein.
The second aspect of the present application also provides a method of transferring a liquid using the PCR tube cap of the first aspect of the present application, comprising:
filling the liquid into a second PCR tube 5, sealing the first PCR tube 4 by the first tube cover 2, and sealing the second PCR tube 5 by the second tube cover 3;
opening the opening of the tube cover outer section 7 of the first ventilation tube 10 and the opening of the tube cover outer section 6 of the second ventilation tube 11;
opening two ends of a liquid channel 12 of the first tube cover 2, and communicating an opening of a tube outer section 6 of the second ventilation tube 11 with one end of the liquid channel 12;
inverting the second PCR tube, introducing gas into the second PCR tube 5 through the first vent tube 10, and pressing the liquid in the second PCR tube 5 into the liquid channel 12;
separating the outer tube cover section 6 of the second vent tube 11 from the liquid channel 12, and closing the two ends of the liquid channel 12;
introducing the liquid in the liquid channel 12 into the first PCR tube by centrifugation;
in some embodiments, the first PCR tube is an octant tube; by adopting the first tube cover, liquid can be evenly split into eight tube cavities of the eight connecting tubes.
In some embodiments, the second PCR tube and the first PCR tube are inverted together, and a gas is introduced into the second PCR tube 5 through the first vent tube 10 to press the liquid in the second PCR tube 5 into the liquid channel 12.
In the application, by inverting the second PCR tube, the liquid is located in the second tube cover 3 and at the top of the second PCR tube 5, the liquid is injected into the liquid channel 12 of the first tube cover along with the air pressure, all the liquid is located in the liquid channel in the horizontal direction due to the gravity, and the liquid cannot easily flow out of the other end of the liquid channel 12 due to the surface tension; after the liquid is transferred to the liquid channel, the plug is timely installed, so that the generation of liquid leakage and aerosol can be prevented. The second vent pipe 11 of the second pipe cover is pulled out from the liquid channel 12, and the end of the liquid channel 12 is plugged by a plug, so that the first PCR pipe is closed, and the pollution of aerosol in the air to the liquid is reduced or avoided; the contamination of air by aerosols that may be generated in the second PCR tube is also reduced or avoided.
In some embodiments, the liquid completes the reaction within the second PCR tube 5 with the opening of the first vent tube cover outer section 7 and the opening of the second vent tube cover outer section 6 closed.
The PCR tube cover is combined with the PCR tube, can be used in different reactions, such as pre-amplification-qPCR, wherein the second PCR tube is used for pre-amplification, pre-amplification products are further mixed in the process of passing through the second vent tube and the liquid channel, the pre-amplification products are packaged into a plurality of tube cavities of the first PCR tube without uncapping, the first PCR tube is provided with freeze-dried probes or primers in advance, and a complete qPCR system is formed with the transferred pre-amplification products, so that the qPCR reaction is completed.
In order to prevent the generation of aerosol, the second PCR tube should be disposed of, for example, in a large amount of clear water or by closing the outer sections of the tube covers of the first and second ventilation tubes with a pair of hot clamps.
The PCR tube cap combined with the PCR tube can also be used for reverse transcription PCR, wherein the second PCR tube is used for reverse transcription reaction, the first PCR tube is provided with a PCR or qPCR reaction system to be reacted in advance, and forms a complete PCR or q PCR system with the transferred reverse transcription product, so that the PCR or qPCR reaction is completed.
The PCR tube cap combined PCR tube can also be used for protein recognition based on antigen-antibody reaction, for example, incubation of sample proteins and various primary antibodies can be completed in a second PCR tube, and the first-step reaction can be completed. The second PCR tube is respectively preloaded with the second antibodies for identifying different antibodies in different tube cavities, and the second PCR tube is correspondingly specifically identified with the first antibodies in the first step reaction, so that the purpose of detecting multiple antigen targets by one sample at one time is achieved.
The PCR tube cap of the present application in combination with the PCR tube can also be used for reactions that generate toxic or irritating gases and require sub-packaging or reduced reaction volumes in the second step.
The PCR tube cap and application of the present application will be described below by way of specific examples.
Example 1 application of the PCR tube cap of the present application in Pre-amplification-qPCR
To detect low copy templates, such as several copies of a pathogen in a body fluid, pathogen nucleic acid templates are often amplified by pre-amplification techniques and then detected or identified by qPCR. The pre-amplification step of this technique is prone to aerosol generation affecting qPCR accuracy, and more serious may cause environmental pollution of the detection chamber, causing serious loss. By adopting the PCR tube cover, the pre-amplification product can be transferred without opening the cover, and qCPR is further completed. The method comprises the following specific steps:
1) Before use, the outer sections 6 and 7 of the two vent pipes of the second pipe cover are in a closed state, the pre-amplification reaction system is filled into the second PCR pipe, and the pre-amplification reaction is completed through 8-15 cycles.
2) After the pre-amplification is finished, the pre-amplification product is gathered at the bottom of the tube by short centrifugation. The ends of the outer tube cap sections 6 and 7 are cut along the broken lines shown in fig. 5, so that the two vent tubes are opened, and the required qPCR reaction solution (without primers or probes) is injected into the outer tube cap section 7 by using a syringe, and the solution is mixed by flicking a small single tube.
3) Adding freeze-dried probes and primers required for qPCR into an octant tube (first PCR tube), and closing the octant tube by using a first tube cover;
4) The plugs 1 at the two ends of the liquid channel 12 of the first tube cover are removed, the outer tube cover section 6 of the second vent tube is quickly inserted into the liquid channel 12 of the first tube cover, the whole assembly is quickly inverted (as shown in fig. 6), liquid in the second PCR tube is accumulated at the tube cover, and air is injected into the second PCR tube from the first vent tube by using a syringe, so that the liquid in the second PCR tube is transferred from the second vent tube 11 to the liquid channel 12. The integral assembly is of transparent material and closely focuses on the position of the liquid advancing, and when the liquid forefront reaches the stopper bottom position (which can be marked in advance), the air injection is stopped and the stopper 1 is reinstalled. The second cap is removed and the plug at one end is closed quickly (as shown in fig. 7) because the plug at one end is closed tightly and no liquid flows out.
5) At this time, a closed space is formed in the eight-linked tube cavity, after being placed in front, the liquid is centrifuged briefly, and is uniformly dispersed into each tube through a longitudinal channel or opening 13 of the liquid channel 12, and the liquid and the freeze-dried probe and the primer which are pre-installed at the bottom of the eight-linked tube form a qPCR reaction system, and finally enter a qPCR link.
6) If residual liquid exists in the second PCR tube, the second PCR tube should be discarded to prevent the generation of aerosol, such as a large amount of clean water or closed-end treatment with a pair of hot clamps.
Comparative example 1
1) The pre-amplification reaction was completed using a commercially available single PCR tube using the same reaction system and control conditions as in example 1.
2) The pre-amplified product was pooled at the bottom of the tube by brief centrifugation.
3) The lyophilized probes and primers required for qPCR were added to the octamer tube.
4) And opening the PCR tube, filling the liquid in the PCR tube into the eight-connecting tube by adopting a liquid-transferring gun, and closing the tube cover of the eight-connecting tube.
5) qPCR reactions were completed using the same reaction conditions as in example 1.
After qPCR is finished, the eight connecting pipes of the embodiment 1 and the comparative embodiment 1 are subjected to short centrifugation, so that all liquid in the pipes is gathered at the bottoms of the pipes, the liquid in the two eight connecting pipes is respectively gathered in the two PCR pipes by using a pipette, and the direct observation shows that the volume of the residual liquid is obviously larger than that of the PCR pipe cover adopting the traditional cover-opening transfer mode, thereby indicating that the volume of the evaporated liquid is smaller, the aerosol generation amount is lower, and the pollution caused by the liquid in the transfer process is effectively reduced; in addition, the transfer process does not have uncapping operation, so that the pollution of aerosol in the air to samples in the PCR tube can be obviously reduced, and the accuracy of the reaction and the result in the PCR tube is improved.

Claims (10)

1. A PCR tube cap comprising a first tube cap and a second tube cap;
the first pipe cover is provided with at least one annular bulge for closing the first PCR pipe, the first pipe cover is internally provided with a liquid channel penetrating through the first pipe cover, and two ends of the liquid channel can be opened or closed; the liquid channel is provided with an opening at the position in the annular bulge and is used for communicating the liquid channel with the first PCR tube;
the second pipe cover is provided with a first vent pipe and a second vent pipe; the inner section of the tube cover of the first vent tube extends downwards, and when the second tube cover seals the second PCR tube, the opening of the inner section of the tube cover of the first vent tube is positioned at the bottom of the second PCR tube; the opening of the inner section of the tube cover of the second ventilation tube is positioned at the top of the second PCR tube; the openings of the outer sections of the tube covers of the first ventilation tube and the second ventilation tube can be opened or closed;
the opening of the outer section of the tube cover of the second vent tube is communicated with one end of the liquid channel in an open state.
2. The PCR tube cap as claimed in claim 1, wherein one end of the liquid channel is sealably connected to the opening of the outer tube cap section of the second vent tube in an open state.
3. The PCR tube cap of claim 1, wherein an inner diameter of one end of the liquid channel is equal to an outer diameter of the second vent tube cap outer section.
4. The PCR tube cap of claim 1, wherein one end of the liquid channel is detachably connected to the tube cap outer section of the second vent tube.
5. The PCR tube cap of claim 1, wherein the tube cap outer section of the second vent tube is perpendicular to the axis of the second PCR tube when the second tube cap closes the second PCR tube.
6. The PCR tube cap as claimed in claim 1, wherein the liquid channel is opened and closed at both ends by a detachable plug or cap.
7. The PCR tube cap as claimed in claim 1, wherein eight annular protrusions are uniformly provided on the first tube cap, and openings are provided at positions of the liquid channel in the annular protrusions for communicating the liquid channel with the first PCR tube.
8. The PCR tube cap of claim 7, wherein the first PCR tube is an octal tube.
9. The PCR tube cap according to any one of claims 1-8, wherein the liquid channel has a volume of 20-100 μl.
10. A method of transferring liquid using the PCR tube cap of any one of claims 1-9, comprising:
filling the liquid into a second PCR tube, sealing the first PCR tube by a first tube cover, and sealing the second PCR tube by a second tube cover;
opening the outer section openings of the tube covers of the first ventilation tube and the second ventilation tube;
opening two ends of a liquid channel of the first tube cover, and communicating an opening of the outer section of the tube cover of the second vent tube with one end of the liquid channel;
inverting the second PCR tube, introducing gas into the second PCR tube through the first vent tube, and pressing the liquid in the second PCR tube into the liquid channel;
separating the outer section of the tube cover of the second vent tube from the liquid channel, and closing the two ends of the liquid channel;
centrifuging to make the liquid in the liquid channel enter the first PCR tube;
preferably, the first PCR tube is an octal tube.
CN202210685345.XA 2022-06-15 2022-06-15 PCR tube cover and application Pending CN117264729A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210685345.XA CN117264729A (en) 2022-06-15 2022-06-15 PCR tube cover and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210685345.XA CN117264729A (en) 2022-06-15 2022-06-15 PCR tube cover and application

Publications (1)

Publication Number Publication Date
CN117264729A true CN117264729A (en) 2023-12-22

Family

ID=89201487

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210685345.XA Pending CN117264729A (en) 2022-06-15 2022-06-15 PCR tube cover and application

Country Status (1)

Country Link
CN (1) CN117264729A (en)

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