CN117214095A - Detection system and protein analyzer based on filtering and amplifying module - Google Patents
Detection system and protein analyzer based on filtering and amplifying module Download PDFInfo
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- CN117214095A CN117214095A CN202311195798.5A CN202311195798A CN117214095A CN 117214095 A CN117214095 A CN 117214095A CN 202311195798 A CN202311195798 A CN 202311195798A CN 117214095 A CN117214095 A CN 117214095A
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- 238000001914 filtration Methods 0.000 title claims abstract description 67
- 238000001514 detection method Methods 0.000 title claims abstract description 52
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 16
- 238000006243 chemical reaction Methods 0.000 claims abstract description 82
- 238000001228 spectrum Methods 0.000 claims abstract description 78
- 230000003321 amplification Effects 0.000 claims abstract description 34
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 34
- 238000002955 isolation Methods 0.000 claims description 27
- 239000003990 capacitor Substances 0.000 claims description 17
- 230000003287 optical effect Effects 0.000 claims description 17
- 230000010354 integration Effects 0.000 claims description 3
- 239000002131 composite material Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000005259 measurement Methods 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
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Abstract
The embodiment of the invention discloses a detection system and a protein analyzer based on a filtering and amplifying module, wherein a processing module is used for simultaneously providing the same pulse modulation signal for a light source module and the filtering and amplifying module. The light source module is used for providing a first wavelength spectrum under the action of a first level signal and is closed under the action of a second level signal, the photoelectric conversion module is used for converting the second wavelength spectrum into a level signal when the light source module is closed, and the filtering amplification module is used for being disconnected under the action of the first level signal and being connected under the action of the second level signal. Therefore, when the light source module works, the photoelectric conversion module comprises the first wavelength spectrum and the second wavelength spectrum, the filtering amplification module is not conducted at the moment, the light source module is turned off, the second wavelength spectrum can be maintained for a certain time, the filtering amplification module is conducted at the moment, namely, the first wavelength spectrum is filtered through the turning off of the light source module, and the detection accuracy is improved.
Description
Technical Field
The invention relates to the technical field of protein detection devices, in particular to a detection system based on a filtering and amplifying module and a protein analyzer.
Background
A specific protein analyzer is an auxiliary instrument for analyzing the content and properties of specific proteins in the body. Most of the specific protein analyzers currently use the principles of turbidimetry and refractometry to achieve the measurement of reagent concentrations. However, the protein analysis method based on nephelometry and refraction method has larger disturbance of light, and thus the accuracy of analysis results is lower.
Disclosure of Invention
The invention provides a detection system and a protein analyzer based on a filtering amplification module, which are used for improving the accuracy of measurement by filtering a first wavelength spectrum.
In a first aspect, an embodiment of the present invention provides a detection system based on a filtering and amplifying module, including a processing module, a filtering and amplifying module, a light source module and a photoelectric conversion module;
the output end of the processing module is respectively and electrically connected with the input end of the light source module and the control end of the filtering and amplifying module and is used for simultaneously providing the same pulse modulation signal for the light source module and the filtering and amplifying module, wherein the pulse modulation signal comprises a first level signal and a second level signal, and the first level signal is larger than the second level signal;
the light source module is used for providing a first wavelength spectrum under the action of the first level signal and is turned off under the action of the second level signal;
the sample module is used for converting the first wavelength spectrum into a second wavelength spectrum;
the photoelectric conversion module is used for converting the second wavelength spectrum into a level signal when the light source module is turned off;
the input end of the filtering and amplifying module is electrically connected with the output end of the photoelectric conversion module, the output end of the filtering and amplifying module is electrically connected with the processing module, and the filtering and amplifying module is used for being disconnected under the action of the first level signal and being conducted under the action of the second level signal, so that the level signal input by the photoelectric conversion module is output to the processing module after passing through the filtering and amplifying module.
Further, the filtering and amplifying module comprises a first amplifying circuit, an integrating circuit, a control circuit and a second amplifying circuit;
the inverting input end of the first amplifying circuit is electrically connected with the output end of the photoelectric conversion module, the output end of the first amplifying circuit is electrically connected with the input end of the integrating circuit, the output end of the integrating circuit is respectively electrically connected with the input end of the control circuit and the in-phase input end of the second amplifying circuit, the output end of the second amplifying circuit is electrically connected with the input end of the processing module, the control end of the control circuit is electrically connected with the input end of the processing module, and the control module is used for being conducted under the action of a first level signal so as to disconnect the integrating circuit from the second amplifying circuit and disconnect the integrating circuit from the second amplifying circuit under the action of a second level signal so as to conduct the integrating circuit from the second amplifying circuit.
Further, the first amplifying circuit comprises a first operational amplifier, the integrating circuit comprises a first resistor and a first capacitor, the second amplifying circuit comprises a second operational amplifier, and the control circuit comprises a triode;
the inverting input end of the first operational amplifier is electrically connected with the output end of the photoelectric conversion module, the non-inverting input end of the first operational amplifier is electrically connected with the grounding end, the output end of the first operational amplifier is electrically connected with the first end of the first resistor, the second end of the first resistor is electrically connected with the first polar plate of the first capacitor, the second polar plate of the first capacitor is respectively electrically connected with the collector electrode of the triode and the non-inverting input end of the second operational amplifier, and the output end of the second operational amplifier is electrically connected with the input end of the processing module;
the emitter of the triode is electrically connected with the grounding end, and the base of the triode is electrically connected with the input end of the processing module.
Further, the control circuit further comprises a second resistor and a third resistor;
the first end of the second resistor is electrically connected with the input end of the processing module, the second end of the second resistor is electrically connected with the first end of the third resistor and the base electrode of the triode respectively, and the second end of the third resistor is electrically connected with the grounding end;
the first amplifying circuit further comprises a fourth resistor, and the second amplifying circuit further comprises a fifth resistor and a sixth resistor;
the first end of the fourth resistor is electrically connected with the output end of the photoelectric conversion module, the second end of the fourth resistor is electrically connected with the reverse input end of the first operational amplifier, the first end of the fifth resistor is electrically connected with the second polar plate of the first capacitor, the second end of the fifth resistor is electrically connected with the non-inverting input end of the second operational amplifier, and the sixth resistor is connected in series between the output end of the second operational amplifier and the input end of the processing module.
Further, the filtering and amplifying module further comprises a first isolation circuit, wherein the first isolation circuit is positioned between the output end of the processing module and the control end of the control circuit, and the first isolation circuit comprises a first optocoupler;
the first end of the first optical coupler is electrically connected with the output end of the processing module, the second end of the first optical coupler is electrically connected with the ground end, the third end of the first optical coupler is electrically connected with the control end of the control circuit, and the fourth end of the first optical coupler is electrically connected with the power supply voltage end.
Further, the detection module further comprises a second isolation circuit, the second isolation circuit is located between the output end of the processing module and the input end of the light source module, and the second isolation circuit comprises a second optocoupler;
the first end of the second optical coupler is electrically connected with the output end of the processing module, the second end of the second optical coupler is electrically connected with the ground end, the third end of the second optical coupler is electrically connected with the input end of the light source module, and the fourth end of the second optical coupler is electrically connected with the power supply voltage end.
Further, the detection system further comprises an analog-to-digital conversion module, and the analog-to-digital conversion module is located between the output end of the filtering and amplifying circuit and the input end of the processing module.
Further, the detection system further comprises a display module, wherein the display module is electrically connected with the output end of the processing module, and the display module is used for displaying image information generated by the processing module according to the level signal of the filtering and amplifying module.
In a second aspect, an embodiment of the present invention further provides a protein analyzer, including the detection system provided in any one of the embodiments of the present invention.
The detection system based on the filtering and amplifying module comprises a processing module, a filtering and amplifying module, a light source module and a photoelectric conversion module. The output end of the processing module is respectively and electrically connected with the input end of the light source module and the control end of the filtering and amplifying module and is used for simultaneously providing the same pulse modulation signal for the light source module and the filtering and amplifying module, wherein the pulse modulation signal comprises a first level signal and a second level signal, and the first level signal is larger than the second level signal. The light source module is used for providing a first wavelength spectrum under the action of a first level signal and is turned off under the action of a second level signal. The sample module is used for converting the first wavelength spectrum into a second wavelength spectrum, and the photoelectric conversion module is used for converting the second wavelength spectrum into a level signal when the light source module is turned off. The input end of the filtering and amplifying module is electrically connected with the output end of the photoelectric conversion module, the output end of the filtering and amplifying module is electrically connected with the processing module, and the filtering and amplifying module is used for being disconnected under the action of a first level signal and being conducted under the action of a second level signal, so that the level signal input by the photoelectric conversion module is output to the processing module after being filtered and amplified. In the above technical scheme, the light source module and the filtering amplification module adopt the same pulse modulation information for control, when the light source module works, a first wavelength spectrum is provided, the first wavelength spectrum is converted into a second wavelength spectrum through the sample module, namely, the photoelectric conversion module comprises the first wavelength spectrum and the second wavelength spectrum, because the filtering amplification module is disconnected, the influence of the first wavelength spectrum on detection is avoided, and when the light source module is closed, the second wavelength spectrum can be maintained for a certain time, namely, the photoelectric conversion module comprises the second wavelength spectrum, the filtering amplification module is conducted, and then level signals input by the photoelectric conversion module are output to the processing module after being filtered and amplified, and the detection system further has higher sensitivity and anti-interference capability by filtering the influence of the first wavelength spectrum on detection at the source.
Drawings
FIG. 1 is a block diagram of a detection system based on a filter amplification module according to an embodiment of the present invention;
fig. 2 is a partial circuit configuration diagram of a detection system based on a filtering amplification module according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be fully described below by way of specific embodiments with reference to the accompanying drawings in the examples of the present invention. It is apparent that the described embodiments are some, but not all, embodiments of the present invention, and that all other embodiments, which a person of ordinary skill in the art would obtain without making inventive efforts, are within the scope of this invention.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present invention and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments of the invention described herein may be implemented in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
Fig. 1 is a block diagram of a detection system based on a filtering and amplifying module according to an embodiment of the present invention, and as shown in fig. 1, the detection system includes a processing module 10, a filtering and amplifying module 20, a light source module 30, and a photoelectric conversion module 40. The output end of the processing module 10 is electrically connected with the input end of the light source module 30 and the control end of the filtering and amplifying module 20, respectively, and is used for simultaneously providing the same pulse modulation signal PWM for the light source module 30 and the filtering and amplifying module 20, wherein the pulse modulation signal PWM comprises a first level signal and a second level signal, and the first level signal is greater than the second level signal. The light source module 30 is configured to provide a first wavelength spectrum under the action of a first level signal and to turn off under the action of a second level signal. The sample module 01 is used to convert the first wavelength spectrum into a second wavelength spectrum. The photoelectric conversion module 40 is configured to convert the second wavelength spectrum into a level signal PMT when the light source module 30 is turned off. The input end of the filtering and amplifying module 20 is electrically connected with the output end of the photoelectric conversion module 40, the output end of the filtering and amplifying module 20 is electrically connected with the processing module 10, and the filtering and amplifying module 20 is used for being disconnected under the action of a first level signal and being conducted under the action of a second level signal, so that a level signal PMT input by the photoelectric conversion module 40 is output to the processing module 10 after passing through the filtering and amplifying module 20.
Specifically, the light source module 30 may include a laser light emitting diode, and the output end of the processing module 10 is electrically connected to the input end of the light source module 30, so that the processing module 10 may provide a light emission control signal for the light source module 30 to make the light source module 30 emit light. The pulse modulation signal PWM includes a first level signal and a second level signal that continuously vary at intervals, wherein the first level signal may be a high level signal and the second level signal may be a low level signal, and when the processing module 10 provides the pulse modulation signal PWM to the light source module 30, the light source module 30 emits light to provide a first wavelength spectrum under the action of the first level signal, and the light source module 30 does not emit light under the action of the second level signal, in other words, the light source module 30 keeps emitting light at intervals under the action of the pulse modulation signal PWM. Sample module 01 is a sample to be tested, for example, sample module 01 may be a protein sample, and the content and nature of a particular protein within the protein sample may be detected by a detection system. The sample module 01 is placed on the light-emitting path of the light source module 30, so that the first wavelength spectrum emitted by the light source module 30 irradiates the sample module 01, and then under the action of the pulse modulation signal PWM, the first wavelength spectrum provided by the light source module 30 and the sample module 01 generate chemical reaction to generate a second wavelength spectrum. The photoelectric conversion module 40 is disposed on the light emitting path of the second wavelength spectrum, and when the light source module 30 is in operation, the receiving end of the photoelectric conversion module 40 includes the composite light (i.e. the second wavelength spectrum generated by the chemical reaction, and the first wavelength spectrum provided by the light source module 30). When the light source module 30 is turned off, the second wavelength spectrum is maintained for a certain time, and thus the receiving end of the photoelectric conversion module 40 includes the second wavelength spectrum generated by the chemical reaction.
In the detection analysis, the second wavelength spectrum is used for detecting the sample module 01, and the first wavelength spectrum may interfere with the detection of the sample module 01. Therefore, the influence of the first wavelength spectrum needs to be filtered, i.e. the optical signal when the light source module 30 is turned off needs to be used as the analysis signal of the processing module 10.
Therefore, the output end of the processing module 10 is electrically connected with the input end of the light source module 30 and the control end of the filter amplification module 20, the same pulse modulation signal PWM is provided for the light source module 30 and the filter amplification module 20 through the processing module 10, the filter amplification module 20 is cut off under the action of the first level signal, the processing module 10 cannot detect the sample module 01, the filter amplification module 20 is turned on under the action of the second level signal, the processing module 01 can detect the sample module 01, and it can be understood that the light source module 30 emits light to provide a first wavelength spectrum under the action of the first level signal, the receiving end of the photoelectric conversion module 40 is a composite light (namely, the second wavelength spectrum generated through chemical reaction and the first wavelength spectrum provided by the light source module 30), at this moment, the filter amplification module 20 is cut off, the processing module 10 cannot detect the sample module 01, at this moment, the receiving end of the photoelectric conversion module 40 is a second wavelength, the photoelectric conversion module 40 converts the second wavelength into the level signal PMT, and transmits the level signal to the filter amplification module 20, and the filter amplification module 20 is turned on after the filter amplification module 10 starts to process the filter amplification module 10. Therefore, the interference of the first wavelength spectrum is filtered at the source, so that the detection system has higher sensitivity and anti-interference capability, and the detection result is more accurate.
It should be noted that, when the light source module 30 is turned on, the receiving end of the photoelectric conversion module 40 is a composite light, and although the photoelectric conversion module 40 can convert the composite light into an electrical signal, the filtering and amplifying module 20 is turned off, that is, the electrical signal cannot be transmitted to the processing module 10, so that in order to better embody the present invention, the photoelectric conversion module 40 is defined to convert the second wavelength spectrum into the level signal PMT when the light source module 30 is turned off. It will be appreciated by those skilled in the art that the photoelectric conversion module 40 is itself used to convert an optical signal into an electrical signal, i.e., the photoelectric conversion module 40 may also convert a composite light into a level signal.
It should be further noted that, the first wavelength spectrum may be an excitation spectrum of 680nm, and the second wavelength spectrum may be an emission spectrum of 610nm, and the specific data of the first wavelength spectrum and the second wavelength spectrum are not limited in the embodiment of the present invention, and may be set by those skilled in the art according to needs.
In summary, the detection system based on the filtering and amplifying module in the invention comprises a processing module, a filtering and amplifying module, a light source module and a photoelectric conversion module. The output end of the processing module is respectively and electrically connected with the input end of the light source module and the control end of the filtering and amplifying module and is used for simultaneously providing the same pulse modulation signal for the light source module and the filtering and amplifying module, wherein the pulse modulation signal comprises a first level signal and a second level signal, and the first level signal is larger than the second level signal. The light source module is used for providing a first wavelength spectrum under the action of a first level signal and is turned off under the action of a second level signal. The sample module is used for converting the first wavelength spectrum into a second wavelength spectrum, and the photoelectric conversion module is used for converting the second wavelength spectrum into a level signal when the light source module is turned off. The input end of the filtering and amplifying module is electrically connected with the output end of the photoelectric conversion module, the output end of the filtering and amplifying module is electrically connected with the processing module, and the filtering and amplifying module is used for being disconnected under the action of a first level signal and being conducted under the action of a second level signal, so that the level signal input by the photoelectric conversion module is output to the processing module after being filtered and amplified. According to the technical scheme, the light source module and the filtering amplification module are controlled by adopting the same pulse modulation information, when the light source module works, the first wavelength spectrum is provided, the first wavelength spectrum is converted into the second wavelength spectrum through the sample module, namely, the photoelectric conversion module comprises the first wavelength spectrum and the second wavelength spectrum, the filtering amplification module is disconnected, so that the influence of the first wavelength spectrum on detection is avoided, when the light source module is closed, the second wavelength spectrum is maintained for a certain time, namely, the photoelectric conversion module comprises the second wavelength spectrum, the filtering amplification module is conducted, and then level signals input by the photoelectric conversion module are filtered and amplified and then are output to the processing module.
Optionally, fig. 2 is a partial circuit structure diagram of a detection system based on a filtering amplification module according to an embodiment of the present invention. Referring to fig. 1 and 2, the filter amplification module 20 includes a first amplification circuit 210, an integration circuit 220, a control circuit 230, and a second amplification circuit 240. The inverting input terminal of the first amplifying circuit 210 is electrically connected to the output terminal of the photoelectric conversion module 40, the output terminal of the first amplifying circuit 210 is electrically connected to the input terminal of the integrating circuit 220, the output terminal of the integrating circuit 220 is electrically connected to the input terminal of the control circuit 240 and the non-inverting input terminal of the second amplifying circuit 240, the output terminal of the second amplifying circuit 240 is electrically connected to the input terminal of the processing module 10, the control terminal of the control circuit 230 is electrically connected to the output terminal of the processing module 10, and the control module 240 is configured to be turned on under the action of the first level signal so as to disconnect the integrating circuit 220 from the second amplifying circuit 240, and to be turned off under the action of the second level signal so as to turn on the integrating circuit 220 from the second amplifying circuit 240.
Specifically, the first amplifying circuit 210 is located at the output end of the photoelectric conversion module 40, that is, the inverting input end of the first amplifying circuit 210 is electrically connected to the output end of the photoelectric conversion module 40, and the output end of the first amplifying circuit 210 is electrically connected to the inverting input end of the first amplifying circuit 210, so that the first amplifying circuit 210 forms a follower circuit, and the level signal output by the photoelectric conversion module 40 is buffered, isolated and the load capacity of the circuit is improved through the first amplifying circuit 210. The input end of the integrating circuit 220 is electrically connected to the output end of the first amplifying circuit 210, and the output end of the integrating circuit 220 is electrically connected to the control circuit 230 and the second amplifying circuit 240, respectively, so that under the action of the first level signal, the light source module 40 emits light to provide a first wavelength spectrum, the control circuit 230 is turned on, at this time, the first amplifying circuit 210, the integrating circuit 220 and the control circuit 230 are communicated, the receiving end of the photoelectric conversion module 40 includes the composite light, and the level signal converted by the photoelectric conversion module 40 is accumulated in the integrating circuit 220 and is not output to the second amplifying circuit 240, i.e. the processing module 10 does not detect.
Under the action of the second level signal, the light source module 40 is turned off, the control circuit 230 is turned off, at this time, the first amplifying circuit 210, the integrating circuit 220 and the second amplifying circuit 240 are connected, the receiving end of the photoelectric conversion module 40 includes a second wavelength spectrum, and then the photoelectric conversion module 40 converts the second wavelength spectrum into a level signal, the level signal is buffered and isolated by the first amplifying circuit 240, the integrating circuit 220 and the second amplifying circuit 240 amplify and output the level signal to the input end of the processing module 10, and the processing module 10 starts to detect, so, the control circuit 230 and the light source module 30 are simultaneously controlled by the same pulse modulation signal PWM, when the light source module 30 is turned off, the control circuit 230 is turned off, so that the filter amplifying module 20 is turned on to perform signal analysis, and the influence of the first wavelength spectrum on detection is filtered at the source, thereby having higher sensitivity and anti-interference capability, and making the detected result more accurate.
Optionally, with continued reference to fig. 1 and 2 based on the above embodiment, the first amplifying circuit 210 includes a first operational amplifier U1, the integrating circuit 220 includes a first resistor R1 and a first capacitor C1, the second amplifying circuit 240 includes a second operational amplifier U2, and the control circuit 230 includes a transistor Q1. The inverting input terminal of the first operational amplifier U1 is electrically connected to the output terminal of the photoelectric conversion module 40, the non-inverting input terminal of the first operational amplifier U1 is electrically connected to the ground terminal GND, the output terminal of the first operational amplifier U1 is electrically connected to the first terminal of the first resistor R1, the second terminal of the first resistor R1 is electrically connected to the first plate of the first capacitor C1, the second plate of the first capacitor C1 is electrically connected to the collector of the triode Q1 and the non-inverting input terminal of the second operational amplifier U2, respectively, and the output terminal of the second operational amplifier U2 is electrically connected to the input terminal of the processing module 10. The emitter of the triode Q1 is electrically connected with the grounding end GND, and the base of the triode Q1 is electrically connected with the input end of the processing module 10.
Specifically, the output end of the processing module 10 is electrically connected to the base of the triode Q1, and then the triode Q1 is turned on under the action of the first level signal, at this time, the light source module 30 emits light to provide a first wavelength spectrum, the receiving end of the photoelectric conversion module 40 includes composite light, and then the level signal converted by the photoelectric conversion module 40 reaches the first resistor R1 after being buffered and isolated at the first operational amplifier U1, and since the second plate of the first capacitor C1 is electrically connected to the ground end GND through the triode Q1 after the triode Q1 is turned on, the level signal converted by the photoelectric conversion module 40 is accumulated at the second plate of the first capacitor C1 and is not output to the second operational amplifier U2, that is, the filtering and amplifying module 20 is turned off at this time, and the processing module 10 does not detect. Under the action of the second level signal, the triode Q1 is turned off, at this time, the light source module 30 is turned off, the receiving end of the photoelectric conversion module 40 includes a second wavelength spectrum, and then the level signal converted by the photoelectric conversion module 40 reaches the first resistor R1 after being buffered and isolated at the first operational amplifier U1, and after the triode Q1 is turned off, the second polar plate of the first capacitor C1 is electrically connected with the non-inverting input end of the second operational amplifier U2, so that the level signal converted by the photoelectric conversion module 40 is amplified by the second operational amplifier U2 and then output to the input end of the processing module 10, that is, the filtering and amplifying module 20 is turned on at this time, and the processing module 10 starts to detect. Therefore, the influence of the first wavelength spectrum on detection is filtered out at the source, so that the detection device has higher sensitivity and anti-interference capability, and the detection result is more accurate.
Optionally, with continued reference to fig. 1 and 2 based on the above embodiments, the control circuit 230 further includes a second resistor R2 and a third resistor R3. The first end of the second resistor R2 is electrically connected to the input end of the processing module 10, the second end of the second resistor R2 is electrically connected to the first end of the third resistor R3 and the base of the triode Q1, and the second end of the third resistor R3 is electrically connected to the ground GND. The first amplifying circuit 210 further includes a fourth resistor R4, and the second amplifying circuit 240 further includes a fifth resistor R5 and a sixth resistor R6. The first end of the fourth resistor R4 is electrically connected to the output end of the photoelectric conversion module 40, the second end of the fourth resistor R4 is electrically connected to the inverting input end of the first operational amplifier U1, the first end of the fifth resistor R5 is electrically connected to the second plate of the first capacitor C1, the second end of the fifth resistor R5 is electrically connected to the non-inverting input end of the second operational amplifier U2, and the sixth resistor R6 is serially connected between the output end of the second operational amplifier U2 and the input end of the processing module 10.
Specifically, the second resistor R2 and the third resistor R3 are disposed between the base and the emitter of the transistor Q1, and the turn-on voltage of the transistor Q1 is set through the second resistor R2 and the third resistor R3. The fourth resistor R4 is disposed between the output end of the photoelectric conversion module 40 and the inverting input end of the first operational amplifier U1, the fifth resistor R5 is disposed between the second polar plate of the first capacitor C1 and the non-inverting input end of the second operational amplifier U2, and the sixth resistor R6 is disposed between the output end of the second operational amplifier U2 and the input end of the processing module 10, so that the voltage division function is achieved through the fourth resistor R4, the fifth resistor R5 and the sixth resistor R6, and the circuit is further protected. It should be noted that, the first amplifying circuit 210, the integrating circuit 220, the control circuit 230, and the second amplifying circuit 240 further include other resistors and capacitors to ensure the normal operation of each device in the circuits, which are not described in detail herein.
Optionally, with continued reference to fig. 1 and 2, the filter amplification module 20 further includes a first isolation circuit 250, the first isolation circuit 50 being located between the output of the processing module 10 and the control terminal of the control circuit 230, the first isolation circuit 250 including a first optocoupler U3. The first end of the first optocoupler U3 is electrically connected to the output end of the processing module 10, the second end of the first optocoupler U3 is electrically connected to the ground GND, the third end of the first optocoupler U3 is electrically connected to the control end of the control circuit 230, and the fourth end of the first optocoupler U3 is electrically connected to the power supply voltage end VCC. Specifically, the pulse modulation signal PWM includes continuously transformed first level information and second level information, and further, by setting the first isolation circuit 250 between the output end of the processing module 10 and the control end of the control circuit 230, the anti-interference capability of the first isolation circuit 250 is stronger, so that the influence of the pulse modulation signal PWM on the measurement result is reduced by the first isolation circuit 250. In addition, the photoelectric conversion module 40 itself also has a large current, and therefore, the influence of the photoelectric conversion module 40 can also be reduced by the first isolation circuit 250. It should be noted that, the first isolation circuit 250 further includes some pull-up resistors and pull-down resistors to ensure the normal operation of the first optocoupler U3, which is not described in detail herein.
Optionally, with continued reference to fig. 1 and 2, the detection module further includes a second isolation circuit 50, the second isolation circuit 50 being located between the output of the processing module 10 and the input of the light source module 30, the second isolation circuit including a second optocoupler U4. The first end of the second optocoupler U4 is electrically connected to the output end of the processing module 10, the second end of the second optocoupler U4 is electrically connected to the ground GND, the third end of the second optocoupler U4 is electrically connected to the input end of the light source module 30, and the fourth end of the second optocoupler U4 is electrically connected to the power supply voltage end VCC. Specifically, the pulse modulation signal PWM includes continuously transformed first level information and second level information, and further, by disposing the second isolation circuit 50 between the output end of the processing module 10 and the input end of the light source module 30, the second isolation circuit 50 has strong anti-interference capability, so that the influence of the pulse modulation signal PWM on the measurement result is reduced by the second isolation circuit 50. In addition, the photoelectric conversion module 40 itself also has a large current, and therefore, the influence of the photoelectric conversion module 40 can also be reduced by the second isolation circuit 50. It should be noted that, the second isolation circuit 50 further includes some pull-up resistors and pull-down resistors to ensure the normal operation of the second optocoupler U4, which is not described in detail herein.
Optionally, with continued reference to fig. 1, the detection system further includes a filter 60, where the filter 60 is disposed in the optical path between the sample module 01 and the photoelectric conversion module 40, and the filter 60 is configured to filter out the first wavelength spectrum. Specifically, the filter 60 may be a bandpass filter, and the filter 60 may further filter out the spectrum of other wavelength ranges through the second wavelength spectrum. The photoelectric conversion module 40 may be a photomultiplier, and then under the action of the second level signal, the light source module 30 is turned off, the second wavelength spectrum generated at the sample module 01 reaches the photoelectric conversion module 40 through the optical filter 60, so that the influence of the first wavelength spectrum on the measurement result can be further reduced, and the measurement accuracy is further improved.
Optionally, with continued reference to fig. 1, the detection system further includes an analog-to-digital conversion module 70, the analog-to-digital conversion module 70 being located between the output of the filter amplification circuit 20 and the input of the processing module 10. Specifically, the final output of the filter amplifying circuit 20 is a level signal, i.e. an analog signal, and the processing module 10 may be a processing chip, i.e. the processing module 10 cannot directly process and display the analog signal, so that the analog signal is converted into a digital signal by the analog-to-digital conversion module 70 through setting the analog-to-digital conversion module 70 at the output end of the filter amplifying circuit 20 and the input end of the processing module 10, thereby ensuring that the processing module 10 can detect.
Optionally, with continued reference to fig. 1, the detection system further includes a display module 80, where the display module 80 is electrically connected to the output end of the processing module 10, and the display module 80 is configured to display the image information generated by the processing module 10 according to the level signal of the filtering amplifying module 20. Specifically, the level signal output by the filtering and amplifying module 20 is converted into a digital signal by the analog-to-digital conversion module 70 and then output to the processing module 10, the processing module 10 starts to detect, and the final detection result is displayed by the display module 80, so that the user can intuitively understand the detection result.
Optionally, with continued reference to fig. 1, the detection system further includes a parameter adjustment module 90, where the parameter adjustment module 90 is electrically connected to an input of the processing module 10, and is configured to adjust parameter information of the pulse modulation signal PWM. Specifically, the frequency and the duty ratio of the pulse modulation signal PWM may be set by the parameter adjusting module 90, and then multiple groups of detection may be performed by changing the frequency and the duty ratio of the pulse modulation signal PWM, so as to improve the accuracy of detection.
Based on the same inventive concept, the embodiment of the present invention further provides a protein analyzer, which includes the detection system described in any one of the embodiments, so that the protein analyzer provided in the embodiment of the present invention has the corresponding beneficial effects in the above embodiment, which is not described herein again.
Note that the above is only a preferred embodiment of the present invention and the technical principle applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, and that various obvious changes, rearrangements, combinations, and substitutions can be made by those skilled in the art without departing from the scope of the invention. Therefore, while the invention has been described in connection with the above embodiments, the invention is not limited to the embodiments, but may be embodied in many other equivalent forms without departing from the spirit or scope of the invention, which is set forth in the following claims.
Claims (10)
1. The detection system based on the filtering and amplifying module is characterized by comprising a processing module, a filtering and amplifying module, a light source module and a photoelectric conversion module;
the output end of the processing module is respectively and electrically connected with the input end of the light source module and the control end of the filtering and amplifying module and is used for simultaneously providing the same pulse modulation signal for the light source module and the filtering and amplifying module, wherein the pulse modulation signal comprises a first level signal and a second level signal, and the first level signal is larger than the second level signal;
the light source module is used for providing a first wavelength spectrum under the action of the first level signal and is turned off under the action of the second level signal;
the sample module is used for converting the first wavelength spectrum into a second wavelength spectrum;
the photoelectric conversion module is used for converting the second wavelength spectrum into a level signal when the light source module is turned off;
the input end of the filtering and amplifying module is electrically connected with the output end of the photoelectric conversion module, the output end of the filtering and amplifying module is electrically connected with the processing module, and the filtering and amplifying module is used for being disconnected under the action of the first level signal and being conducted under the action of the second level signal, so that the level signal input by the photoelectric conversion module is output to the processing module after passing through the filtering and amplifying module.
2. The detection system of claim 1, wherein the filter amplification module comprises a first amplification circuit, an integration circuit, a control circuit, and a second amplification circuit;
the inverting input end of the first amplifying circuit is electrically connected with the output end of the photoelectric conversion module, the output end of the first amplifying circuit is electrically connected with the input end of the integrating circuit, the output end of the integrating circuit is respectively electrically connected with the input end of the control circuit and the in-phase input end of the second amplifying circuit, the output end of the second amplifying circuit is electrically connected with the input end of the processing module, the control end of the control circuit is electrically connected with the output end of the processing module, and the control module is used for being conducted under the action of a first level signal so as to disconnect the integrating circuit from the second amplifying circuit and disconnect the integrating circuit from the second amplifying circuit under the action of a second level signal so as to conduct the integrating circuit from the second amplifying circuit.
3. The detection system of claim 2, wherein the first amplification circuit comprises a first operational amplifier, the integration circuit comprises a first resistor and a first capacitor, the second amplification circuit comprises a second operational amplifier, and the control circuit comprises a transistor;
the inverting input end of the first operational amplifier is electrically connected with the output end of the photoelectric conversion module, the non-inverting input end of the first operational amplifier is electrically connected with the grounding end, the output end of the first operational amplifier is electrically connected with the first end of the first resistor, the second end of the first resistor is electrically connected with the first polar plate of the first capacitor, the second polar plate of the first capacitor is respectively electrically connected with the collector electrode of the triode and the non-inverting input end of the second operational amplifier, and the output end of the second operational amplifier is electrically connected with the input end of the processing module;
the emitter of the triode is electrically connected with the grounding end, and the base of the triode is electrically connected with the input end of the processing module.
4. The detection system of claim 3, wherein the control circuit further comprises a second resistor and a third resistor;
the first end of the second resistor is electrically connected with the input end of the processing module, the second end of the second resistor is electrically connected with the first end of the third resistor and the base electrode of the triode respectively, and the second end of the third resistor is electrically connected with the grounding end;
the first amplifying circuit further comprises a fourth resistor, and the second amplifying circuit further comprises a fifth resistor and a sixth resistor;
the first end of the fourth resistor is electrically connected with the output end of the photoelectric conversion module, the second end of the fourth resistor is electrically connected with the reverse input end of the first operational amplifier, the first end of the fifth resistor is electrically connected with the second polar plate of the first capacitor, the second end of the fifth resistor is electrically connected with the non-inverting input end of the second operational amplifier, and the sixth resistor is connected in series between the output end of the second operational amplifier and the input end of the processing module.
5. The detection system of claim 2, wherein the filter amplification module further comprises a first isolation circuit, the first isolation circuit being located between the output of the processing module and the control terminal of the control circuit, the first isolation circuit comprising a first optocoupler;
the first end of the first optocoupler is electrically connected with the output end of the processing module, the second end of the first optocoupler is electrically connected with the grounding end, the third end of the first optocoupler is electrically connected with the control end of the control circuit, and the fourth end of the first optocoupler is electrically connected with the power supply voltage end.
6. The detection system of claim 1, wherein the detection module further comprises a second isolation circuit located between the output of the processing module and the input of the light source module, the second isolation circuit comprising a second optocoupler;
the first end of the second optical coupler is electrically connected with the output end of the processing module, the second grounding end of the second optical coupler is electrically connected with the input end of the light source module, the third end of the second optical coupler is electrically connected with the fourth end of the second optical coupler is electrically connected with the power supply voltage end.
7. The detection system of claim 1, further comprising a filter disposed in the optical path between the sample module and the photoelectric conversion module, the filter configured to filter out the first wavelength spectrum.
8. The detection system of claim 1, further comprising an analog-to-digital conversion module located between the output of the filter amplification circuit and the input of the processing module.
9. The detection system according to claim 1, further comprising a display module electrically connected to an output of the processing module, the display module configured to display image information generated by the processing module according to the level signal of the filtering and amplifying module.
10. A protein analyser comprising a detection system according to any one of claims 1 to 9.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311195798.5A CN117214095A (en) | 2023-09-15 | 2023-09-15 | Detection system and protein analyzer based on filtering and amplifying module |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311195798.5A CN117214095A (en) | 2023-09-15 | 2023-09-15 | Detection system and protein analyzer based on filtering and amplifying module |
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|---|---|
| CN117214095A true CN117214095A (en) | 2023-12-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202311195798.5A Pending CN117214095A (en) | 2023-09-15 | 2023-09-15 | Detection system and protein analyzer based on filtering and amplifying module |
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| Country | Link |
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| CN (1) | CN117214095A (en) |
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- 2023-09-15 CN CN202311195798.5A patent/CN117214095A/en active Pending
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