CN117192140A - Sperm sample automatic processing analysis method and system - Google Patents
Sperm sample automatic processing analysis method and system Download PDFInfo
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- CN117192140A CN117192140A CN202311322073.8A CN202311322073A CN117192140A CN 117192140 A CN117192140 A CN 117192140A CN 202311322073 A CN202311322073 A CN 202311322073A CN 117192140 A CN117192140 A CN 117192140A
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- 238000012545 processing Methods 0.000 title claims abstract description 40
- 238000004458 analytical method Methods 0.000 title claims description 30
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- 239000011521 glass Substances 0.000 claims description 42
- 238000001514 detection method Methods 0.000 claims description 22
- 238000012546 transfer Methods 0.000 claims description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 230000004083 survival effect Effects 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 120
- 210000004027 cell Anatomy 0.000 description 6
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 5
- 210000000278 spinal cord Anatomy 0.000 description 3
- 230000000747 cardiac effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
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- 230000002457 bidirectional effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Abstract
The application discloses an automatic sperm sample processing and analyzing method and system, belonging to the field of sperm medical treatment.
Description
Technical Field
The application relates to the medical field, in particular to an automatic sperm sample processing and analyzing method and system.
Background
Currently, the conventional sperm sample detection method mainly relies on manual operation of a tester, and results are interpreted after complicated operation by observing under a microscope. Therefore, the sperm analysis result is greatly influenced by the individual level of the inspector, the analysis accuracy is low, and strict and objective data support is lacked.
The application aims to develop an automatic sperm sample processing and analyzing method and system, which replace manual operation and manual interpretation of detection personnel, reduce manual operation errors and improve the accuracy of detection results.
Disclosure of Invention
In order to overcome the defects of the prior art, one of the purposes of the application is to provide an automatic sperm sample processing and analyzing method which reduces the artificial operation error and improves the accuracy of the detection result.
In order to overcome the defects of the prior art, the second aim of the application is to provide an automatic sperm sample processing and analyzing system which reduces the artificial operation error and improves the accuracy of the detection result.
One of the purposes of the application is realized by adopting the following technical scheme:
an automatic sperm sample processing and analyzing method comprises the following steps:
transferring a sample to be tested: placing a sample to be tested in a sample injection area, and enabling a system to sense the sample to be tested and transfer the sample to a sample detection area;
obtaining the volume of a sample to be measured: weighing a sample to be measured, and calculating the volume of the sample to be measured according to the weight data;
judging the liquefaction degree: carrying out liquefaction analysis on a sample to be tested, judging the liquefaction degree of the sample to be tested, and carrying out film-making and sample adding after the liquefaction is completed; when the liquefaction is not completed, waiting to enable the sample to be tested to continue to be liquefied and continuing to carry out liquefaction analysis until the liquefaction is completed;
slide glass is manufactured: transferring the blank slide glass to a smear region, filling a sample and a reagent on the blank slide glass, completing smear, and transferring the slide glass to an image detection region;
manufacturing a counting pool: transferring the blank counting pool to a sample adding area, adding a sample into the blank counting pool, and transferring the counting pool after sample adding to an image detection area;
collecting an image: and collecting images of the glass slide and the counting pool, and analyzing the images to obtain the vitality and the survival rate of the sperm sample.
Further, in the step of collecting an image, collecting an image of the slide and the counting chamber includes the steps of:
acquiring an initial image of the slide or the counting chamber;
judging the definition of the initial image;
adjusting the position of the camera according to the definition of the initial image until the definition of the initial image meets the requirement;
and the slide glass or the counting pool after the image is acquired moves out of the view field of the camera, and the slide glass or the image of the counting pool without the image is moved into the view field of the camera for image acquisition.
Further, the method further comprises an information identification step, wherein the information identification step is positioned between the transferring sample to be tested step and the obtaining sample volume to be tested, and the information identification step specifically comprises the following steps: and controlling the sample to be tested to rotate, scanning the label on the sample to be tested, and identifying the information of the sample to be tested.
The second purpose of the application is realized by adopting the following technical scheme:
the utility model provides a sperm sample automated processing analysis system for implement above-mentioned sperm sample automated processing analysis method, includes sample introduction identification module, sample processing module, film-making module, preparation counting cell module, image acquisition module and controller, sample introduction identification module, sample processing module, film-making module, preparation counting cell module, image acquisition module respectively with controller communication connection, sample introduction identification module discerns sample to be detected of sample introduction region, sample processing module is right sample to be detected weighs and judges the liquefaction degree, the controller control film-making module with preparation counting cell module carries out the smear to the sample to be detected that liquefaction is accomplished respectively and fills blank counting cell and form slide and counting cell, image acquisition module gathers slide and the image of counting cell.
Further, the device also comprises a transfer module which is in communication connection with the controller, and transfers the sample to be detected from the sample injection area to the image detection area, and the position of the slide glass and the counting pool relative to the image acquisition module is moved so that the image acquisition module can acquire images of the slide glass and the counting pool respectively.
Further, the film-making module includes filling module, smear module, the filling module is with sample and reagent filling to blank slide glass on, smear module is with sample and reagent evenly scribble on the blank slide glass.
Further, the smear module comprises a rotating structure and a horizontal moving structure connected with the rotating structure.
Further, the device also comprises a storage module, wherein the storage module is positioned at one side of the image acquisition module and is used for storing the glass slide after the image acquisition and the counting pool.
Further, the device also comprises an information identification module, wherein the information identification module comprises a rotating module and a code scanning module, the rotating module drives the sample to be tested to rotate, and the code scanning module scans the label on the sample to be tested to identify the information of the sample to be tested.
Compared with the prior art, the sperm sample automatic processing analysis system automatically realizes the steps of transferring a sample to be detected, acquiring the volume of the sample to be detected, judging the liquefaction degree, manufacturing a glass slide, manufacturing a counting pool, collecting images and the like through the sample injection identification module, the sample processing module, the film-making module, the manufacturing counting pool module, the image acquisition module and the controller, and obtains the data of the volume of the sperm sample, the vitality and the activity rate of the sperm sample and the like so as to replace manual operation and manual interpretation of a detector, reduce the manual operation error and improve the accuracy of a detection result.
Drawings
FIG. 1 is a flow chart of an automated sperm sample processing analysis method of the present application;
FIG. 2 is a block diagram of an automated sperm sample processing analysis system of the present application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
The application relates to an automatic sperm sample processing analysis method, which is based on the principle that cerebrospinal fluid around the spinal cord protects internal spinal tissue, and the flow parameters of the cerebrospinal fluid can reflect the compression degree of the spinal cord. The spinal cord can thus be monitored indirectly by monitoring the flow parameters of the cerebrospinal fluid, which beats with the heartbeat, so that the cerebrospinal fluid has a characteristic motion signal synchronized with the cardiac cycle. The cerebrospinal fluid is influenced by the cardiac cycle and has the physiological characteristic of periodical bidirectional flow, and the flow drives the membrane structures at two sides to beat at the same time.
FIG. 1 is a view showing an automatic sperm sample processing analysis method according to the present application, comprising the steps of:
transferring a sample to be tested: placing a sample to be tested in a sample injection area, and enabling a system to sense the sample to be tested and transfer the sample to a sample detection area;
obtaining the volume of a sample to be measured: weighing a sample to be measured, and calculating the volume of the sample to be measured according to the weight data;
judging the liquefaction degree: carrying out liquefaction analysis on a sample to be tested, judging the liquefaction degree of the sample to be tested, and carrying out film-making and sample adding after the liquefaction is completed; when the liquefaction is not completed, waiting to enable the sample to be tested to continue to be liquefied and continuing to carry out liquefaction analysis until the liquefaction is completed;
slide glass is manufactured: transferring the blank slide glass to a smear region, filling a sample and a reagent on the blank slide glass, completing smear, and transferring the slide glass to an image detection region;
manufacturing a counting pool: transferring the blank counting pool to a sample adding area, adding a sample into the blank counting pool, and transferring the counting pool after sample adding to an image detection area;
collecting an image: and collecting images of the glass slide and the counting pool, and analyzing the images to obtain the vitality and the survival rate of the sperm sample.
Specifically, when transporting the sample that awaits measuring, judge the concrete position that the sample cup is located through the position sensor, when the sample was placed at the sampling region, the position sensor sensed and sent the response signal to the treater, and the treater sends the instruction, and the rotatory module of control sample recognizer drives the sample and rotates, sweeps the sign indicating number module simultaneously and accomplishes information identification to upload to the treater, sample transport module starts, transports the sample to sample detection region, carries out follow-up detection.
The sample detection area is provided with a weight measurer, and after the sample is transported, a weight signal is collected and sent to the controller. The controller calculates the sperm sample volume according to the weight signal, and records the sperm sample volume, and the sperm sample volume is used as an index for evaluating the whole condition of the sperm sample.
The sperm sample can be liquefied continuously along with time, and the liquefaction degree analysis is needed before the subsequent operation, so that the complete liquefaction of the sperm is ensured. Specifically, after the sperm sample is weighed, the controller issues a command to the liquefaction degree analyzer to judge the liquefaction degree, if the liquefaction degree is insufficient, the controller executes a waiting command to enable the sperm to continue to be liquefied, and the liquefaction degree analysis is continued until the liquefaction is completed.
In the slide making step, the controller issues an instruction, the transfer module is controlled to transfer the blank counting pool to the slide making area, the injector absorbs samples and reagents and fills the samples and the reagents onto the blank slide, the smear module comprises a rotating structure and a horizontal moving structure connected with the rotating structure, and the samples and the reagents are uniformly smeared on the blank slide through the rotating motion and the horizontal motion.
In the step of collecting images, collecting images of the slide glass and the counting chamber comprises the following steps:
acquiring an initial image of the slide or the counting chamber;
judging the definition of the initial image;
adjusting the position of the camera according to the definition of the initial image until the definition of the initial image meets the requirement;
and the slide glass or the counting pool after the image is acquired moves out of the view field of the camera, and the slide glass or the image of the counting pool without the image is moved into the view field of the camera for image acquisition.
With continued reference to fig. 2, the present application further relates to an automatic sperm sample processing and analyzing system, which is configured to implement the automatic sperm sample processing and analyzing method, and includes a sample injection identification module, a sample processing module, a film-making module, a counting pool making module, an image acquisition module, and a controller, where the sample injection identification module, the sample processing module, the film-making module, the counting pool making module, and the image acquisition module are respectively in communication with the controller, the sample injection identification module identifies a sample to be detected in a sample injection area, the sample processing module weighs the sample to be detected and determines the liquefaction degree, the controller controls the film-making module and the counting pool making module to smear and fill the liquefied sample to be detected into a blank counting pool to form a glass slide and a counting pool, and the image acquisition module acquires images of the glass slide and the counting pool.
Specifically, the sperm sample automatic processing analysis system further comprises a transfer module, an information identification module and a storage module, wherein the transfer module is in communication connection with the controller, the transfer module transfers the sample to be detected from the sample injection area to the image detection area, and the position of the slide glass and the counting pool relative to the image acquisition module is moved, so that the image acquisition module can acquire images of the slide glass and the counting pool respectively. The information identification module comprises a rotating module and a code scanning module, the rotating module drives the sample to be tested to rotate, and the code scanning module scans the label on the sample to be tested to identify the information of the sample to be tested. The storage module is positioned at one side of the image acquisition module and is used for storing the glass slide after the image acquisition and the counting pool.
Specifically, the film-making module includes filling module, smear module, and filling module is with sample and reagent filling to blank slide glass on, smear module evenly smears sample and reagent on blank slide glass. The smear module comprises a rotating structure and a horizontal moving structure connected with the rotating structure.
Compared with the prior art, the sperm sample automatic processing analysis system automatically realizes the steps of transferring a sample to be detected, acquiring the volume of the sample to be detected, judging the liquefaction degree, manufacturing a glass slide, manufacturing a counting pool, collecting images and the like through the sample injection identification module, the sample processing module, the film-making module, the manufacturing counting pool module, the image acquisition module and the controller, and obtains the data of the volume of the sperm sample, the vitality and the activity rate of the sperm sample and the like so as to replace manual operation and manual interpretation of a detector, reduce the manual operation error and improve the accuracy of a detection result.
The foregoing examples illustrate only a few embodiments of the application, which are described in detail and are not to be construed as limiting the scope of the application. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present application, which are equivalent to the above embodiments according to the essential technology of the present application, and these are all included in the protection scope of the present application.
Claims (9)
1. An automatic sperm sample processing and analyzing method is characterized by comprising the following steps:
transferring a sample to be tested: placing a sample to be tested in a sample injection area, and enabling a system to sense the sample to be tested and transfer the sample to a sample detection area;
obtaining the volume of a sample to be measured: weighing a sample to be measured, and calculating the volume of the sample to be measured according to the weight data;
judging the liquefaction degree: carrying out liquefaction analysis on a sample to be tested, judging the liquefaction degree of the sample to be tested, and carrying out film-making and sample adding after the liquefaction is completed; when the liquefaction is not completed, waiting to enable the sample to be tested to continue to be liquefied and continuing to carry out liquefaction analysis until the liquefaction is completed;
slide glass is manufactured: transferring the blank slide glass to a smear region, filling a sample and a reagent on the blank slide glass, completing smear, and transferring the slide glass to an image detection region;
manufacturing a counting pool: transferring the blank counting pool to a sample adding area, adding a sample into the blank counting pool, and transferring the counting pool after sample adding to an image detection area;
collecting an image: and collecting images of the glass slide and the counting pool, and analyzing the images to obtain the vitality and the survival rate of the sperm sample.
2. The automated sperm sample processing analysis method of claim 1, wherein: in the step of collecting images, collecting images of the slide glass and the counting chamber comprises the following steps:
acquiring an initial image of the slide or the counting chamber;
judging the definition of the initial image;
adjusting the position of the camera according to the definition of the initial image until the definition of the initial image meets the requirement;
and the slide glass or the counting pool after the image is acquired moves out of the view field of the camera, and the slide glass or the image of the counting pool without the image is moved into the view field of the camera for image acquisition.
3. The automated sperm sample processing analysis method of claim 1, wherein: the method further comprises an information identification step, wherein the information identification step is positioned between the transferring sample to be tested step and the obtaining sample volume to be tested, and the information identification step specifically comprises the following steps: and controlling the sample to be tested to rotate, scanning the label on the sample to be tested, and identifying the information of the sample to be tested.
4. An automated sperm sample processing analysis system for performing the automated sperm sample processing analysis method of any one of claims 1 to 3, wherein: including advance kind identification module, sample processing module, film-making module, preparation count pond module, image acquisition module and controller, advance kind identification module, sample processing module, film-making module, preparation count pond module, image acquisition module respectively with controller communication connection, advance kind identification module discernment advance the regional sample of waiting to detect, sample processing module is right wait to detect the sample and weigh and judge the liquefaction degree, the controller control film-making module with preparation count pond module carries out the smear and marks blank count pond respectively to the sample of waiting to detect that liquefaction is accomplished and forms slide and count pond, image acquisition module gathers the slide with the image of count pond.
5. The automated sperm sample processing analysis system of claim 4, wherein: the transfer module is in communication connection with the controller, and transfers the sample to be detected from the sample injection area to the image detection area, and the position of the slide glass and the counting pool relative to the image acquisition module is moved so that the image acquisition module can acquire images of the slide glass and the counting pool respectively.
6. The automated sperm sample processing analysis system of claim 4, wherein: the film-making module comprises a filling module and a smear module, wherein the filling module is used for filling samples and reagents onto blank glass slides, and the smear module is used for uniformly smearing the samples and the reagents onto the blank glass slides.
7. The automated sperm sample processing analysis system of claim 6, wherein: the smear module comprises a rotating structure and a horizontal moving structure connected with the rotating structure.
8. The automated sperm sample processing analysis system of claim 4, wherein: the device also comprises a storage module, wherein the storage module is positioned at one side of the image acquisition module and is used for storing the glass slide after the image acquisition and the counting pool.
9. The automated sperm sample processing analysis system of claim 4, wherein: the device comprises a sample to be tested, and is characterized by further comprising an information identification module, wherein the information identification module comprises a rotating module and a code scanning module, the rotating module drives the sample to be tested to rotate, and the code scanning module scans labels on the sample to be tested to identify information of the sample to be tested.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311322073.8A CN117192140A (en) | 2023-10-12 | 2023-10-12 | Sperm sample automatic processing analysis method and system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311322073.8A CN117192140A (en) | 2023-10-12 | 2023-10-12 | Sperm sample automatic processing analysis method and system |
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| Publication Number | Publication Date |
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| CN117192140A true CN117192140A (en) | 2023-12-08 |
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|---|---|---|---|
| CN202311322073.8A Pending CN117192140A (en) | 2023-10-12 | 2023-10-12 | Sperm sample automatic processing analysis method and system |
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| Country | Link |
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| CN (1) | CN117192140A (en) |
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- 2023-10-12 CN CN202311322073.8A patent/CN117192140A/en active Pending
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