CN117177763A - 用于增强疫苗接种期间的t细胞启动的病毒构建体 - Google Patents

用于增强疫苗接种期间的t细胞启动的病毒构建体 Download PDF

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Publication number
CN117177763A
CN117177763A CN202280021291.5A CN202280021291A CN117177763A CN 117177763 A CN117177763 A CN 117177763A CN 202280021291 A CN202280021291 A CN 202280021291A CN 117177763 A CN117177763 A CN 117177763A
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acid sequence
amino acid
rmva
peptide
seq
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CN202280021291.5A
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Chinese (zh)
Inventor
M·J·豪泽
A·多米
G·古铁雷斯
J·帕努奇
V·科特莱亚
T·法雷斯
P·布翁滕波
C·布劳恩
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Laiduosi Co
Geovax Inc
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Laiduosi Co
Geovax Inc
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CN202280021291.5A 2021-02-02 2022-02-02 用于增强疫苗接种期间的t细胞启动的病毒构建体 Pending CN117177763A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163144834P 2021-02-02 2021-02-02
US63/144,834 2021-02-02
PCT/US2022/014970 WO2022169895A1 (en) 2021-02-02 2022-02-02 Viral constructs for use in enhancing t-cell priming during vaccination

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CN117177763A true CN117177763A (zh) 2023-12-05

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CN202280021291.5A Pending CN117177763A (zh) 2021-02-02 2022-02-02 用于增强疫苗接种期间的t细胞启动的病毒构建体

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US (1) US20240344082A1 (https=)
EP (1) EP4288076A4 (https=)
JP (1) JP2024505274A (https=)
KR (1) KR20240001116A (https=)
CN (1) CN117177763A (https=)
AU (1) AU2022215566A1 (https=)
BR (1) BR112023015467A2 (https=)
CA (1) CA3206004A1 (https=)
IL (1) IL304805A (https=)
MX (1) MX2023009008A (https=)
TW (1) TW202246514A (https=)
WO (1) WO2022169895A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117904069A (zh) * 2024-01-04 2024-04-19 华中农业大学 VirEN蛋白介导的DNA拼接和基因编辑方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111533785B (zh) * 2020-02-11 2022-03-08 北京市肿瘤防治研究所 靶向免疫检查点tim3结合肽及其应用
EP4103231A4 (en) 2020-02-14 2024-03-27 Geovax, Inc. VACCINES AND THEIR USES TO INDUCE AN IMMUNE RESPONSE AGAINST SARS-COV2
WO2024206243A1 (en) * 2023-03-24 2024-10-03 Geovax. Inc. Pan-betacoronavirus vaccines and uses thereof
WO2025072383A1 (en) * 2023-09-25 2025-04-03 The Broad Institute, Inc. Viral open reading frames, uses thereof, and methods of detecting the same
GB202409284D0 (en) * 2024-06-27 2024-08-14 Univ Oxford Innovation Ltd Mrna platform

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7045313B1 (en) 1982-11-30 2006-05-16 The United States Of America As Represented By The Department Of Health And Human Services Recombinant vaccinia virus containing a chimeric gene having foreign DNA flanked by vaccinia regulatory DNA
ATE315660T1 (de) 2002-05-16 2006-02-15 Bavarian Nordic As Intergene regionen als insertionsstellen im genom von modifiziertem vaccinia virus ankara (mva)
DK2062023T4 (en) 2006-08-25 2017-02-20 The Government Of The Us Secretary Dept Of Health And Human Services INTERGENIC SITES BETWEEN CONSERVATED GENES THROUGH MODIFIED VACCINIA ANKARA (VAT) VACCINIAVIRUS
US20100305002A1 (en) 2009-04-27 2010-12-02 Roswell Park Cancer Institute Reagents and Methods for Producing Bioactive Secreted Peptides
DK2488649T3 (da) 2009-10-16 2019-06-17 Us Health Rekombinant modificeret vaccinia ankara (mva)-vaccinia-virus indeholdende restrukturerede insertionssteder
RU2696312C2 (ru) * 2014-07-16 2019-08-01 Трансген Са Онколитический вирус для экспрессии модуляторов иммунологических контрольных точек
CN115300622A (zh) * 2015-02-25 2022-11-08 纪念斯隆-凯特琳癌症中心 使用灭活的修饰的痘苗病毒安卡拉作为实体肿瘤的单一免疫疗法或与免疫检查点阻断剂组合
US10799555B2 (en) 2016-09-15 2020-10-13 Leidos, Inc. PD-1 peptide inhibitors
US10098950B2 (en) 2016-09-15 2018-10-16 Leidos, Inc. PD-1 Peptide Inhibitors
JP2019535311A (ja) 2016-11-02 2019-12-12 デイビッド エバンス, 合成キメラポックスウイルス
WO2018218137A1 (en) 2017-05-25 2018-11-29 Leidos, Inc. Pd-1 and ctla-4 dual inhibitor peptides
EP3796891A4 (en) * 2018-04-11 2022-04-20 Precision Molecular Inc. THERAPEUTIC CONSTRUCTS FOR TREATMENT OF CANCER
EP3788142A4 (en) 2018-05-02 2022-04-27 Tonix Pharma Holdings Limited SYNTHETIC CHIMERIC VACCINE VIRUS
CA3168836A1 (en) 2020-02-03 2021-08-12 City Of Hope Poxvirus-based vectors produced by natural or synthetic dna and uses thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117904069A (zh) * 2024-01-04 2024-04-19 华中农业大学 VirEN蛋白介导的DNA拼接和基因编辑方法

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EP4288076A4 (en) 2025-01-22
CA3206004A1 (en) 2022-08-11
JP2024505274A (ja) 2024-02-05
TW202246514A (zh) 2022-12-01
BR112023015467A2 (pt) 2023-10-10
AU2022215566A9 (en) 2025-01-30
IL304805A (en) 2023-09-01
MX2023009008A (es) 2023-10-05
AU2022215566A1 (en) 2023-08-17
WO2022169895A1 (en) 2022-08-11
US20240344082A1 (en) 2024-10-17
KR20240001116A (ko) 2024-01-03
EP4288076A1 (en) 2023-12-13

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