CN117165421A

CN117165421A - Embryo in-vitro retrieval device

Info

Publication number: CN117165421A
Application number: CN202311177149.2A
Authority: CN
Inventors: 张乐; 苏来; 陈迈
Original assignee: Xixian New District Guoruiyinuo Drug Safety Evaluation Research Co ltd
Current assignee: Xixian New District Guoruiyinuo Drug Safety Evaluation Research Co ltd
Priority date: 2023-09-13
Filing date: 2023-09-13
Publication date: 2023-12-05

Abstract

The invention relates to the technical field of embryo retrieval, and discloses an embryo in-vitro retrieval device, which comprises an air film type embryo retrieval structure and a piston type air guide structure, wherein the air film type embryo retrieval structure and the piston type air guide structure are arranged in a main longitudinal reciprocating cavity, the inside of the air film type embryo in-vitro retrieval device is provided with a main piston which can axially move along the main longitudinal reciprocating cavity, and in the moving process, the air pressure of the space above a flaky elastic air film is reduced, and meanwhile, the air pressure of the peripheral area of an annular elastic air film is increased. The embryo in-vitro picking device can suck in an in-vitro embryo into a specific area by utilizing the adsorption capacity of gas under a negative pressure state, and can seal the opening end of the embryo storage area by utilizing the capacity of gas circulation flow when sucking the embryo, thereby preventing the occurrence of the dripping phenomenon of the embryo in the transfer process, and simultaneously effectively blocking external pollutants by the closed area, and reducing the infection probability in the embryo picking process.

Description

Embryo in-vitro retrieval device

Technical Field

The invention relates to the technical field of embryo retrieval, in particular to an embryo in-vitro retrieval device.

Background

Currently, in vitro studies or clinical work on mammalian embryos, workers have generally performed in vitro embryo retrieval operations through embryo aspiration tubing. Embryo suction tubes are divided into oral suction tubes and hand suction tubes. Wherein, the traditional hand suction tube generally uses a Pasteur suction tube with a silica gel suction head, and realizes the in-vitro detection of the embryo by pressing the silica gel suction head; the traditional oral siphon is formed by connecting a hose and an embryo glass needle. The mouth suction pipe has the advantages of simple and convenient operation, rapidness, convenient control and the like, and is adopted by most workers.

For example, chinese patent publication No. CN206843460U discloses an embryo in vitro retrieval device, whose main structure includes a silica gel suction head, a mouth suction head, a first connecting tube, a second connecting tube, an embryo glass needle, a T-shaped three-way ball valve; the T-shaped three-way ball valve is respectively communicated with one end of the first connecting pipe, one end of the second connecting pipe and the embryo glass needle; the other end of the first connecting pipe is communicated with the silica gel suction head; the other end of the second connecting pipe is communicated with the mouth-sucking head. The embryo in-vitro picking device provided by the embryo in-vitro picking device combines two operation modes of oral suction and hand suction; during embryo picking or moving operation, adjusting the sucking mode, sucking the embryo into the embryo glass needle in a sucking mode; when the embryo is picked up to another culture dish, the mode is adjusted to a hand suction mode, and the embryo is placed in the culture dish by pressing the silica gel suction head. The method can avoid the pollution to the embryo caused by direct contact of harmful components such as gas blown out from the mouth of an operator or bacteria or viruses in saliva and the like with the embryo culture solution, and influence the development of the embryo.

For another example, chinese patent with publication number of CN217202669U discloses an in vitro horse embryo detecting device, which mainly comprises a pressing bag, wherein the output end of the pressing bag is fixedly connected with a rectangular three-way pipe, the output ends at two sides of the rectangular three-way pipe are respectively and fixedly connected with a right connecting pipe and a left connecting pipe, the bottom ends of the right connecting pipe and the left connecting pipe are respectively provided with an oral suction head and a glass needle, and the inner side of the top of the rectangular three-way pipe is fixedly sleeved with a rotating shaft; this external device of retrieving of horse embryo through the setting of each subassembly of rectangle three-way pipe inboard, can adjust the position of two sets of whirl boards through the rotatory handle, and then can make right connecting tube or left connecting tube be linked together with the trachea respectively to can reach the effect of opening suction head and glass needle substep operation, and then make the device possess the effect of operation opening suction head or glass needle simultaneously, solved current device and need constantly change corresponding apparatus complex operation and the lower problem of efficiency, further, omit the operation of changing the utensil and also can reduce the infection probability of embryo retrieval in-process.

It is apparent from the above description that: when the embryo in-vitro picking device adsorbs embryos, the mouth suction head is used for sucking the embryos in the culture dish, after the embryos are sucked into the glass needle, the hand suction mode is switched, the embryos are placed in the culture dish by pressing the suction head, in the actual conversion process, the space in the glass needle can be changed to a certain extent due to the change of the internal position of the T-shaped three-way ball valve, the embryos in the glass needle can be changed in longitudinal height, hidden danger that the embryos drop along the suction port exists, and in addition, the pollution degree of the embryos in the transfer process can be increased undoubtedly due to the fact that the suction end in the glass needle is in an opening state.

Disclosure of Invention

The invention provides an embryo in-vitro picking device, which can suck in an in-vitro embryo into a specific area by utilizing the adsorption capacity of gas under a negative pressure state, and can close the opening end of an embryo storage area by utilizing the capacity of gas circulation flow when sucking the embryo, thereby preventing the dripping phenomenon of the embryo in the transfer process, effectively blocking external pollutants by using the closed area, reducing the infection probability in the embryo picking process, and solving the technical problems.

In order to achieve the above purpose, the present invention provides the following technical solutions: the embryo in-vitro retrieving device comprises a columnar hollow shell, a hollow rod structure arranged at the top end of the columnar hollow shell, a component embedding groove arranged at the bottom end of the columnar hollow shell, a main funnel-shaped cavity arranged at the top end of the component embedding groove, a main longitudinal reciprocating cavity arranged at the top end of the main funnel-shaped cavity, a gas flow channel arranged in the columnar hollow shell and communicated with the top end of the main longitudinal reciprocating cavity and the bottom of the columnar hollow shell, and a shaft body perforation arranged in the hollow rod structure, and also comprises a gas film embryo retrieving structure, wherein the gas film embryo retrieving structure is internally provided with a flaky elastic gas film which is upwards deformed under the condition of upper gas negative pressure, sucks embryos into a deformation concave area and generates deformation towards the central direction after the periphery is subjected to gas pressure, and blocks the central area from being closed; and the piston type gas guide structure is arranged in the main longitudinal reciprocating cavity, and a main piston capable of axially moving along the main longitudinal reciprocating cavity is arranged in the piston type gas guide structure, so that the gas pressure of the space above the sheet-shaped elastic gas film is reduced in the moving process, and the gas pressure of the peripheral area of the annular elastic gas film is increased.

Preferably, the gas film type embryo picking structure comprises an embryo suction needle, the top end of the embryo suction needle is provided with a cylindrical bulge structure which can be inserted into the part embedding groove, the circumferential side surface of the cylindrical bulge structure is provided with a sealing ferrule, the center of the embryo suction needle is provided with an embryo suction hole communicated with the bottom of the embryo suction needle, the inside of the cylindrical bulge structure is provided with a rod body inserting groove with an open top end and a bottom end communicated with the embryo suction hole, the inside of the embryo suction needle is provided with an annular gas compression space near the periphery of the open bottom end of the embryo suction hole, the upper end face of the embryo suction needle is provided with a plurality of integrated structures at the periphery of the cylindrical bulge structure and can be correspondingly inserted into an inserting rod structure in the bottom end of the gas flow channel, the inside of the inserting rod structure and the embryo suction needle is provided with a first gas flow hole communicated with the space above the inserting rod structure and the annular gas compression space, the embryo suction needle is intersected with an annular elastic gas film located above the annular elastic gas film embedded in the embryo suction hole.

Preferably, the sealing collar may be inserted into the component insertion groove, and after the insertion, the sealing collar may seal the installation gap.

Preferably, the annular elastic air film and the flaky elastic air film are made of elastic materials which are easy to deform.

Preferably, the piston type gas guiding structure comprises a moving shaft which is inserted into the shaft body perforation and can axially move along the shaft body perforation, a main piston is arranged at one end of the moving shaft, which is positioned in the main longitudinal reciprocating cavity, a main funnel-shaped boss structure matched with the main funnel-shaped cavity is arranged at the bottom end of the main piston, a cylindrical valve rod which is of an integrated structure and can be inserted into a rod body inserting groove is arranged at the bottom end of the main funnel-shaped boss structure, an array-shaped wing-shaped mounting plate is arranged at the end part, which is positioned above the hollow rod structure, a sector-shaped notch is formed in the area between every two wing-shaped structures in the array-shaped wing-shaped mounting plate, a pull rod is arranged at the center of the top end of the array-shaped wing-shaped mounting plate, and a pull plate is arranged at the top end of the pull rod.

Preferably, a sealing hoop is clamped on the circumferential side surface of the main piston, and the shaft body through hole is embedded into a sealing ring at the periphery of the movable shaft.

Preferably, the device further comprises a gas-limited reset structure, the gas-limited reset structure comprises an annular hollow shell, a central sleeve hole capable of being fixedly sleeved on the periphery of the hollow rod structure is arranged in the center of the annular hollow shell, an annular gas flow space is arranged in the bottom area of an annular solid area of the annular hollow shell, three annular array type auxiliary longitudinal reciprocating cavities are arranged in the annular hollow shell in the solid area above the annular gas flow space, a second gas flow hole communicated with the bottom end of the auxiliary longitudinal reciprocating cavity and the top end of the annular gas flow space is arranged in the annular hollow shell, a part mounting hole is arranged in the annular hollow shell in the solid area above the annular gas flow space, the bottom end of the part mounting hole is communicated with the top end of the annular gas flow space, the top end of the part mounting hole is in an opening state, an auxiliary piston capable of axially moving along the auxiliary longitudinal reciprocating cavity is arranged in the annular hollow shell, a telescopic rod penetrating through the annular hollow shell top structure is arranged in the upper end face of the auxiliary longitudinal reciprocating cavity, the top end of each telescopic rod is respectively connected with a fin-shaped telescopic rod in a longitudinal spring in a telescopic sleeve in a telescopic structure in a longitudinal state, and the telescopic rod is fixedly arranged on the inner surface of the main rod body.

Preferably, the circumferential side surface of the auxiliary piston is clamped with a sealing hoop.

Preferably, the device further comprises an elastic gas flow control structure, the elastic gas flow control structure comprises a cylindrical hollow body which can be fixedly arranged in the part mounting hole, sealing glue is filled in the cylindrical hollow body and the part mounting hole at the mounting gap, a longitudinal hollow cavity is arranged in the cylindrical hollow body, a third gas flow hole is formed in the bottom end of the longitudinal hollow cavity, an inverted auxiliary funnel-shaped cavity is formed in the bottom end of the third gas flow hole, the bottom end of the auxiliary funnel-shaped cavity is communicated with the top end of the annular gas flow space, a plurality of communicating longitudinal hollow cavities and a fourth gas flow hole which is arranged in the space above the cylindrical hollow body are formed in the top of the cylindrical hollow body, a longitudinal rod body which can longitudinally move and is matched with the shape of the auxiliary funnel-shaped cavity is arranged in the interior of the cylindrical hollow body, the longitudinal rod body radius of the longitudinal rod body is smaller than that of the third gas flow hole is arranged at the bottom end of the third gas flow hole, a longitudinal rod body is arranged at the top end of the longitudinal rod body, a movable sleeve is arranged at the top end face of the hollow rod body, a movable sleeve is arranged at the top end face of the movable sleeve of the hollow rod body, and the movable sleeve is arranged at the top end face of the movable sleeve, and the movable sleeve is arranged at the top end face of the movable sleeve is arranged at the movable sleeve of the movable sleeve.

Preferably, in the initial state, the elastic strength of the auxiliary coil spring is greater than the sum of the elastic strengths of the three main coil springs.

Compared with the prior art, the invention provides an embryo in-vitro detection device, which has the following beneficial effects:

the embryo in-vitro picking device can suck in an in-vitro embryo into a specific area by utilizing the adsorption capacity of gas under a negative pressure state, and can seal the opening end of the embryo storage area by utilizing the capacity of gas circulation flow when sucking the embryo, thereby preventing the occurrence of the dripping phenomenon of the embryo in the transfer process, and simultaneously effectively blocking external pollutants by the closed area, and reducing the infection probability in the embryo picking process.

Drawings

FIG. 1 is a schematic diagram of the present invention in full section;

FIG. 2 is a schematic diagram of a full section of a gas film embryo retrieval structure according to the present invention;

FIG. 3 is a perspective view of a circular elastic membrane and a sheet-like elastic membrane of the present invention;

FIG. 4 is a perspective view of a piston type gas guiding structure according to the present invention;

FIG. 5 is a perspective view of a gas-limited reset structure of the present invention;

FIG. 6 is a perspective cross-sectional view of a gas-limited reset structure in accordance with the present invention;

FIG. 7 is a perspective view of an elastic gas flow control structure according to the present invention;

FIG. 8 is a perspective cross-sectional view of an elastic gas flow control structure according to the present invention.

Wherein: 1. a columnar hollow housing; 2. a hollow rod structure; 3. a component insertion groove; 4. a main funnel-shaped cavity; 5. a main longitudinal reciprocating cavity; 6. a gas flow channel; 7. perforating the shaft body; 8. an air film embryo retrieval structure; 81. an embryo aspiration needle; 82. a columnar bump structure; 83. a sealing collar; 84. embryo suction holes; 85. the rod body is inserted into the groove; 86. an annular gas compression space; 87. an insertion rod structure; 88. a first gas flow aperture; 89. a circular elastic air film; 810. a sheet-like elastic air film; 9. a piston type gas guiding structure; 91. a movable shaft; 92. a main piston; 93. an array-like fin mounting plate; 94. a main funnel-shaped boss structure; 95. a cylindrical valve stem; 96. a fan-shaped notch; 97. a pull rod; 98. pulling a plate; 10. a gas-limited reset structure; 101. an annular hollow housing; 102. a central trepanning; 103. an annular gas flow space; 104. a second gas flow hole; 105. a secondary longitudinal reciprocating cavity; 106. a slave piston; 107. a telescopic rod; 108. a main coil spring; 109. a component mounting hole; 11. an elastic gas flow control structure; 111. a cylindrical hollow body; 112. a longitudinal hollow cavity; 113. a third gas flow aperture; 114. a secondary funnel-shaped cavity; 115. a fourth gas flow aperture; 116. a movable plate; 117. a push rod; 118. a secondary coil spring; 119. a secondary funnel-shaped boss structure; 1110. a push plate; 1111. a longitudinal rod body.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Referring to fig. 1, an embryo in-vitro retrieving device includes a cylindrical hollow shell 1, a hollow rod structure 2 disposed at the top end of the cylindrical hollow shell 1, a component embedding groove 3 disposed at the bottom end of the cylindrical hollow shell 1, a main funnel-shaped cavity 4 disposed at the top end of the component embedding groove 3, a main longitudinal reciprocating cavity 5 disposed at the top end of the main funnel-shaped cavity 4, a gas flow channel 6 disposed inside the cylindrical hollow shell 1 and communicating the top end of the main longitudinal reciprocating cavity 5 with the bottom of the cylindrical hollow shell 1, and a shaft perforation 7 disposed inside the hollow rod structure 2, wherein during operation, gas can flow along the main longitudinal reciprocating cavity 5 and the gas flow channel 6, thereby realizing a circulating flow effect of the gas, and of course, the space of the main longitudinal reciprocating cavity 5 needs to be large enough, otherwise, the compression or adsorption of the gas will not reach a predetermined requirement.

In order to realize the functions of sucking, blocking and discharging embryos, please refer to fig. 1, 2 and 3, an air film type embryo picking structure 8 is required to be arranged, an upward deformation is generated in the air film type embryo picking structure by the negative pressure state of the air above, the embryos are sucked into the annular elastic air film 810 in the deformed concave area, the annular elastic air film 89 deforms towards the center after the periphery is subjected to the air pressure, the central area is blocked and closed, when the air pressure in the upper area of the annular elastic air film 810 is reduced to a negative pressure value below the atmospheric pressure, the external atmospheric pressure can enable the annular elastic air film 810 to deform upwards, so that the embryos are sucked into the area of the annular elastic air film 810, the embryos are sucked, meanwhile, when the periphery of the annular elastic air film 89 is influenced by the high-pressure air, the annular elastic air film 89 is enabled to change towards the central direction until the annular hole of the annular elastic air film 89 is closed, the blocking effect on the embryos can be realized, and when the air influencing the negative pressure and the high-pressure area is compensated, the annular elastic air film 810 can reset according to self elasticity, so that the embryos can be transferred into the area with the negative pressure value below the atmospheric pressure, the embryo can be prevented from dropping into the closed area, and the closed end can be prevented from being polluted, and the embryo can be effectively dropped into the closed area.

With respect to the specific structure of the air film type embryo picking up structure 8, please refer to fig. 2 and 3, comprising an embryo sucking needle 81, the top end of the embryo sucking needle 81 is provided with a cylindrical protrusion structure 82 which can be inserted into the part embedding groove 3, the circumferential side surface of the cylindrical protrusion structure 82 is provided with a sealing collar 83, in order to realize separable assembly, thereby facilitating the replacement of the part, the sealing collar 83 can be inserted into the part embedding groove 3, and after the insertion, the sealing collar 83 can seal the installation gap, the center of the embryo sucking needle 81 is provided with an embryo sucking hole 84 communicating with the bottom thereof, the inside of the cylindrical protrusion structure 82 is provided with a rod body inserting groove 85 with the top end opened and the bottom end communicated with the embryo sucking hole 84, an annular gas compression space 86 is arranged at the periphery of the embryo suction needle 81 near the bottom opening end of the embryo suction hole 84, a plurality of integrated structures are arranged at the periphery of the columnar bulge structure 82 on the upper end surface of the embryo suction needle 81, a plurality of inserting rod structures 87 which can be correspondingly inserted into the bottom end of the gas flow channel 6 are arranged at the periphery of the columnar bulge structure 82, a first gas flow hole 88 which is communicated with the space above the inserting rod structure 87 and the annular gas compression space 86 is arranged inside the inserting rod structure 87 and the embryo suction needle 81, an annular elastic gas film 89 is embedded at the annular intersection of the embryo suction needle 81 and the annular gas compression space 86, a sheet-shaped elastic gas film 810 which is arranged above the annular elastic gas film 89 is embedded inside the embryo suction needle 81, in order to have deformation capability, thereby playing different roles, the annular elastic air film 89 and the sheet elastic air film 810 are made of elastic materials which are easy to deform, and when the device is in operation, the bottom opening end of the embryo suction hole 84 is required to be immersed into an embryo, and the immersion depth of the embryo suction hole 84 is required to ensure that the device cannot be contacted with air in the suction process.

In order to achieve the cyclic driving effect on the gas in the structure, referring to fig. 1 and 4, a piston type gas guiding structure 9 is required to be disposed in the main longitudinal reciprocating cavity 5, and is disposed therein to be capable of moving axially along the main longitudinal reciprocating cavity 5, and in the moving process, the gas pressure in the upper space of the sheet-shaped elastic gas film 810 is reduced, and meanwhile, the gas pressure in the peripheral area of the annular elastic gas film 89 is increased by the main piston 92, and when the main piston 92 moves upwards in the main longitudinal reciprocating cavity 5, the space in the lower area of the main piston 92 is increased, so that the gas pressure in the upper space of the sheet-shaped elastic gas film 810 is reduced, and meanwhile, the upper area of the main piston 92 is reduced, so that the air above the main piston 92 flows into the annular gas compressing space 86 through the gas flow channel 6 and the first gas flow hole 88, and further, the gas pressure in the peripheral area of the annular elastic gas film 89 is increased, thereby achieving the cyclic driving effect on the gas in the structure and having the convenience of linkage and operation.

With respect to the specific structure of the piston type gas guiding structure 9, please refer to fig. 4, the piston type gas guiding structure comprises a moving shaft 91 which is inserted into the shaft body through hole 7 and can axially move along the shaft body through hole 7, the moving shaft 91 is provided with a main piston 92 at one end located in the main longitudinal reciprocating cavity 5, in order to prevent gas from flowing along the part moving gap, the circumferential side surface of the main piston 92 is required to be clamped with a sealing hoop, the shaft body through hole 7 is embedded with a sealing ring at the periphery of the moving shaft 91, the bottom end of the main piston 92 is provided with a main funnel-shaped boss structure 94 matched with the main funnel-shaped cavity 4, the bottom end of the main funnel-shaped boss structure 94 is provided with a cylindrical valve rod 95 which is of an integrated structure and can be inserted into the rod inserting groove 85, the moving shaft 91 is provided with an array-shaped wing mounting plate 93 at one end located above the hollow rod structure 2, a region between every two wing structures in the array-shaped wing mounting plate 93 forms a fan-shaped notch 96, the center of the array-shaped wing mounting plate 93 is provided with a sealing ring 97, the top end of the array-shaped wing mounting plate 93 is provided with a main funnel-shaped boss structure 94, the main funnel-shaped boss structure 94 is matched with the main funnel-shaped boss structure 94, and the cylindrical valve rod 95 can have a cylindrical shape with improved flow performance.

In order to realize stable discharge capability of embryos, please refer to fig. 1, 5 and 6, a gas-limited reset structure 10 is required, and regarding the specific structure of the gas-limited reset structure 10, please refer to fig. 5 and 6, the device comprises an annular hollow shell 101, a central sleeve hole 102 capable of being fixedly sleeved on the periphery of a hollow rod structure 2 is arranged at the center of the annular hollow shell 101, an annular gas flow space 103 is arranged at the bottom area of an annular solid area of the annular hollow shell 101, three annular array type auxiliary longitudinal reciprocating cavities 105 are arranged in the solid area above the annular gas flow space 103 in the interior of the annular hollow shell 101, a second gas flow hole 104 for communicating the bottom end of the auxiliary longitudinal reciprocating cavities 105 with the top end of the annular gas flow space 103 is arranged in the interior of the annular hollow shell 101, the inside of the annular hollow shell 101 is provided with a component mounting hole 109 in a solid area above the annular gas flowing space 103, the bottom end of the component mounting hole 109 is communicated with the top end of the annular gas flowing space 103, the top end is in an open state, a secondary piston 106 capable of axially moving along the secondary longitudinal reciprocating cavity 105 is arranged in each secondary longitudinal reciprocating cavity 105, in order to prevent gas from flowing along a movable clearance part, a sealing hoop is clamped on the circumferential side surface of the secondary piston 106, the upper end surface of each secondary piston 106 is provided with a telescopic rod 107 penetrating through the top structure of the annular hollow shell 101, the top end of each telescopic rod 107 is fixedly connected with the bottom surface of one fin structure of the array-shaped fin-shaped mounting plate 93 through a connecting plate, each telescopic rod 107 is sleeved with a main spiral spring 108 in a compressed state on a rod body positioned in the auxiliary longitudinal reciprocating cavity 105, when the pull plate 98 is pulled upwards, the telescopic rods 107 are driven to move upwards, so that the three main spiral springs 108 are compressed again, the compressed main spiral springs 108 generate an energy storage state, when an embryo is discharged, the pull plate 98 is released, the energy storage state of the main spiral springs 108 is released, a reset extension phenomenon is generated, and the reset process has elasticity, so that the embryo can be discharged on the basis of buffering effect, and the stable discharge capacity is realized.

In order to realize the control of embryo discharge speed, please refer to fig. 1, 7 and 8, an elastic gas flow control structure 11 is required, with respect to the specific structure of the elastic gas flow control structure 11, please refer to fig. 7 and 8, a cylindrical hollow body 111 is included, which can be fixedly placed inside a component mounting hole 109, and the cylindrical hollow body 111 and the component mounting hole 109 are filled with sealant at a mounting gap, a longitudinal hollow cavity 112 is provided inside the cylindrical hollow body 111, a third gas flow hole 113 is provided at the bottom end of the longitudinal hollow cavity 112 of the cylindrical hollow body 111, an inverted auxiliary funnel-shaped cavity 114 is provided at the bottom end of the third gas flow hole 113 of the cylindrical hollow body 111, and the bottom end of the auxiliary funnel-shaped cavity 114 is communicated with the top end of the annular gas flow space 103, a plurality of fourth gas flow holes 115 are provided at the top of the cylindrical hollow body 111, which are communicated with the longitudinal hollow cavity 112 and the space above the cylindrical hollow body 111, a secondary funnel-shaped boss structure 119 which can move longitudinally and is matched with the structure of the secondary funnel-shaped cavity 114 is arranged in the secondary funnel-shaped cavity 114, a longitudinal rod body 1111 which is positioned in the third gas flow hole 113 and the longitudinal hollow cavity 112 is arranged at the top end of the secondary funnel-shaped boss structure 119, the rod body radius of the longitudinal rod body 1111 is smaller than the structure radius of the third gas flow hole 113, a movable plate 116 is arranged at the top end of the longitudinal rod body 1111 which is positioned in the longitudinal hollow cavity 112, the structure radius of the longitudinal hollow cavity 112 is larger than the structure radius of the movable plate 116, a secondary spiral spring 118 which is in a compressed state is sleeved on the outer periphery of the rod body positioned in the longitudinal hollow cavity 112, in order to ensure the effective control capability of the gas tightness, in an initial state, the elastic strength of the auxiliary spiral spring 118 is greater than the sum of the elastic strengths of the three main spiral springs 108, a moving gap is formed between the upper end surface of the movable plate 116 and the top end of the longitudinal hollow cavity 112, a push rod 117 penetrating through the center structure of the top of the cylindrical hollow body 111 is mounted on the upper end surface of the movable plate 116, a push plate 1110 is mounted on the top end of the push rod 117, when the embryo is sucked, downward pressure needs to be applied to the push plate 1110, a certain gap exists between the auxiliary funnel-shaped boss structure 119 and the conical surface of the auxiliary funnel-shaped cavity 114, at this time, external gas can enter the annular gas flow space 103 along the fourth gas flow hole 115 and the gap to form a high-pressure interval, after the embryo is sucked, the pressure to the push plate 1110 is withdrawn, under the action of the auxiliary spiral spring 118, the gap between the auxiliary funnel-shaped boss structure 119 and the conical surface of the auxiliary funnel-shaped cavity 114 is closed, so that the high pressure gas is sealed inside the annular gas flow space 103, the sealed high pressure gas can effectively prevent the secondary piston 106 from moving downwards, thereby preventing the phenomenon of insufficient gas pressure caused by the downward movement of the primary piston 92, preventing the occurrence of embryo dripping and the phenomenon of contact with the outside, and when the embryo needs to be discharged, the downward pressure is applied to the push plate 1110, so that a certain gap exists between the secondary funnel-shaped boss structure 119 and the conical surface of the secondary funnel-shaped cavity 114, at this time, the high pressure gas inside the annular gas flow space 103 can be discharged to the outside along the gap, and the primary piston 92 can move downwards under the action of the primary coil spring 108, realizing the discharge of the embryo, and in the discharging process, by controlling the size of the gap between the secondary funnel-shaped boss structure 119 and the conical surface of the secondary funnel-shaped cavity 114, the speed of the high-pressure gas discharged outwards can be controlled, and the embryo discharging speed can be controlled.

In use, the bottom opening end of the embryo suction hole 84 is immersed in the embryo, the pulling plate 98 is pulled upwards, the space of the area below the main piston 92 is increased, thus the air pressure of the space above the sheet-shaped elastic air film 810 is reduced, meanwhile, the area above the main piston 92 is reduced, the air above the main piston 92 flows into the annular air compression space 86 through the air flow channel 6 and the first air flow hole 88, the air pressure of the peripheral area of the annular elastic air film 89 is further increased, in the process, when the air pressure of the area above the sheet-shaped elastic air film 810 is reduced to a negative pressure value below the atmospheric pressure, the external atmospheric pressure can cause the sheet-shaped elastic air film 810 to deform upwards, thereby sucking the embryo into the area where the sheet-shaped elastic air film 810 is recessed, realizing the suction of the embryo, meanwhile, when the periphery of the annular elastic air film 89 is affected by high-pressure air, the annular elastic air film 89 is enabled to change towards the center direction until the annular hole part of the annular elastic air film 89 is closed, the blocking effect on the embryo can be achieved, the device is held by hand, the embryo suction hole 84 is aligned to the part needing to be discharged, downward pressure is applied to the push plate 1110, a certain gap exists between the conical surfaces of the auxiliary funnel-shaped boss structure 119 and the auxiliary funnel-shaped cavity 114, at the moment, the high-pressure air in the annular air flow space 103 can be discharged to the outside space along the gap, the main piston 92 can move downwards under the action of the main spiral spring 108, and meanwhile, the sheet-shaped elastic air film 810 can reset according to self elasticity, so that the embryo can obtain the discharging function.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an external retrieval device of embryo, including column hollow shell (1), set up in hollow pole structure (2) on column hollow shell (1) top, set up in part embedded groove (3) of column hollow shell (1) bottom, set up in main funnel-shaped cavity (4) on part embedded groove (3) top, set up in main longitudinal reciprocal cavity (5) on main funnel-shaped cavity (4) top, set up in the inside gas flow passageway (6) and the axle body perforation (7) that communicate main longitudinal reciprocal cavity (5) top and column hollow shell (1) bottom of column hollow shell (1), its characterized in that: and also comprises

The gas film type embryo picking structure (8) is internally provided with a flaky elastic gas film (810) which is subjected to upward deformation under the condition of upper gas negative pressure and sucks the embryo into a deformation concave area, and a ring-shaped elastic gas film (89) which is subjected to gas pressure at the periphery and deforms towards the central direction and blocks the central area;

and a piston type gas guiding structure (9) which is arranged in the main longitudinal reciprocating cavity (5), wherein the main piston (92) which can axially move along the main longitudinal reciprocating cavity (5) and reduce the air pressure in the space above the sheet-shaped elastic air film (810) and increase the air pressure in the peripheral area of the annular elastic air film (89) is arranged in the main longitudinal reciprocating cavity.

2. An embryo in vitro retrieving device according to claim 1, wherein: the gas film type embryo picking structure (8) comprises an embryo suction needle (81), a cylindrical bulge structure (82) which can be inserted into a part embedding groove (3) is arranged at the top end of the embryo suction needle (81), a sealing ferrule (83) is arranged on the circumferential side surface of the cylindrical bulge structure (82), an embryo suction hole (84) which is communicated with the bottom of the embryo suction needle (81) is arranged at the center of the embryo suction needle (81), a rod body inserting groove (85) which is provided with an opening at the top end and is communicated with the embryo suction hole (84) at the bottom end is arranged in the cylindrical bulge structure (82), an annular gas compression space (86) is arranged in the embryo suction needle (81) at the periphery which is close to the opening end at the bottom of the embryo suction hole (84), a plurality of integrated structures are arranged at the periphery of the embryo suction needle (81) and can be correspondingly inserted into an inserting rod structure (87) in the bottom end of a gas flow channel (6), a first annular gas compression space (88) which is communicated with the space above the inserting rod structure (87) and the embryo suction needle (81) is arranged in the embryo suction needle (81), an annular gas compression space (88) is embedded into the annular gas compression space (84), the embryo suction needle (81) is embedded with a sheet-shaped elastic air film (810) positioned above the annular elastic air film (89) in the embryo suction hole (84).

3. An embryo in vitro retrieving device according to claim 2, wherein: the sealing ring (83) can be inserted into the component insertion groove (3), and after insertion, the sealing ring (83) can seal the installation gap.

4. An embryo in vitro retrieving device according to claim 2, wherein: the annular elastic air film (89) and the flaky elastic air film (810) are made of elastic materials which are easy to deform.

5. An embryo in vitro retrieving device according to claim 2, wherein: the piston type gas guiding structure (9) comprises a moving shaft (91) which is inserted into a shaft body perforation (7) and can axially move along the shaft body perforation (7), a main piston (92) is arranged at one end, located inside a main longitudinal reciprocating cavity (5), of the moving shaft (91), a main funnel-shaped boss structure (94) matched with the main funnel-shaped cavity (4) is arranged at the bottom end of the main piston (92), a cylindrical valve rod (95) which is of an integrated structure and can be inserted into a rod body inserting groove (85) is arranged at the bottom end of the main funnel-shaped boss structure (94), an array-shaped wing-shaped mounting plate (93) is arranged at the end part, located above the hollow rod structure (2), a sector-shaped notch (96) is formed in the area between every two wing-shaped structures in the array-shaped wing-shaped mounting plate (93), a pull rod (97) is arranged at the center of the top end of the array-shaped wing-shaped mounting plate (93), and a pull plate (98) is arranged at the top end of the pull rod (97).

6. An embryo in vitro retrieving device according to claim 5, wherein: the circumferential side surface of the main piston (92) is clamped with a sealing hoop, and the shaft body through hole (7) is embedded with a sealing ring at the periphery of the movable shaft (91).

7. An embryo in vitro retrieving device according to claim 5, wherein: the gas-restricted type resetting structure (10) is further included, the gas-restricted type resetting structure (10) comprises an annular hollow shell (101), a central sleeve hole (102) which can be fixedly sleeved on the periphery of the hollow rod structure (2) is arranged in the center of the annular hollow shell (101), an annular gas flow space (103) is arranged in the bottom area of an annular solid area of the annular hollow shell (101), three auxiliary longitudinal reciprocating cavities (105) which are arranged in an annular array mode are arranged in the solid area above the annular gas flow space (103) in the inside of the annular hollow shell (101), a second gas flow hole (104) which is communicated with the bottom end of each auxiliary longitudinal reciprocating cavity (105) and the top end of the annular gas flow space (103) is arranged in the inside of the annular hollow shell (101), a component mounting hole (109) is arranged in the solid area above the annular gas flow space (103), the bottom end of the component mounting hole (109) is communicated with the top end of the annular gas flow space (103), the top end of each auxiliary longitudinal reciprocating cavity (105) is in an opening state, one auxiliary longitudinal reciprocating cavity (105) can be axially arranged on each auxiliary piston (106) of the annular hollow rod structure (101) in a penetrating mode, the top end of each telescopic rod (107) is fixedly connected with the bottom surface of one fin-shaped structure of the array-shaped fin-shaped mounting plate (93) through a connecting plate, and each telescopic rod (107) is sleeved with a main spiral spring (108) in a compressed state on a rod body positioned in the auxiliary longitudinal reciprocating cavity (105).

8. An embryo in vitro retrieving device according to claim 7, wherein: the circumferential side of the auxiliary piston (106) is clamped with a sealing hoop.

9. An embryo in vitro retrieving device according to claim 7, wherein: the elastic gas flow control structure (11) is further included, the elastic gas flow control structure (11) comprises a cylindrical hollow body (111) which can be fixedly arranged in the component mounting hole (109), the cylindrical hollow body (111) and the component mounting hole (109) are filled with sealant at a mounting gap, a longitudinal hollow cavity (112) is arranged in the cylindrical hollow body (111), a third gas flow hole (113) is arranged at the bottom end of the longitudinal hollow cavity (112), an inverted auxiliary funnel-shaped cavity (114) is arranged at the bottom end of the cylindrical hollow body (111) at the third gas flow hole (113), the bottom end of the auxiliary funnel-shaped cavity (114) is communicated with the top end of the annular gas flow space (103), a plurality of fourth gas flow holes (115) which are communicated with the longitudinal hollow cavity (112) and the space above the cylindrical hollow body (111) are arranged at the top of the cylindrical hollow body (111), a third gas flow hole (113) is arranged in the auxiliary funnel-shaped cavity (111), the auxiliary funnel-shaped cavity (114) can longitudinally move and is matched with the auxiliary funnel-shaped structure (114), the longitudinal funnel-shaped auxiliary funnel-shaped cavity (119) is arranged at the top end of the third gas flow hole (119) and is arranged at the top end of the auxiliary funnel-shaped structure (119), the rod radius of the longitudinal rod body (1111) is smaller than the structure radius of the third gas flow hole (113), the movable plate (116) is arranged at the top end of the longitudinal hollow cavity (112), the structure radius of the longitudinal hollow cavity (112) is larger than that of the movable plate (116), the auxiliary spiral spring (118) in a compressed state is sleeved on the periphery of the rod body positioned in the longitudinal hollow cavity (112), a moving gap exists between the upper end face of the movable plate (116) and the top end of the longitudinal hollow cavity (112), a push rod (117) penetrating through the center structure of the top of the cylindrical hollow body (111) is arranged at the upper end face of the movable plate (116), and a push plate (1110) is arranged at the top end of the push rod (117).

10. An embryo in vitro retrieving device according to claim 9, wherein: in an initial state, the elastic strength of the auxiliary spiral spring (118) is larger than the sum of the elastic strengths of the three main spiral springs (108).