CN117143722A - Multichannel frozen cell synchronous thawing device and control method thereof - Google Patents
Multichannel frozen cell synchronous thawing device and control method thereof Download PDFInfo
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- CN117143722A CN117143722A CN202310918192.3A CN202310918192A CN117143722A CN 117143722 A CN117143722 A CN 117143722A CN 202310918192 A CN202310918192 A CN 202310918192A CN 117143722 A CN117143722 A CN 117143722A
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- 238000010257 thawing Methods 0.000 title claims abstract description 95
- 230000001360 synchronised effect Effects 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims description 18
- 238000007710 freezing Methods 0.000 claims abstract description 44
- 230000008014 freezing Effects 0.000 claims abstract description 44
- 238000010438 heat treatment Methods 0.000 claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 28
- 238000005138 cryopreservation Methods 0.000 claims abstract description 11
- 230000005540 biological transmission Effects 0.000 claims abstract description 10
- 239000000523 sample Substances 0.000 claims description 24
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 238000012544 monitoring process Methods 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 4
- 239000004065 semiconductor Substances 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims 2
- 238000001816 cooling Methods 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 239000012595 freezing medium Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/85—Food storage or conservation, e.g. cooling or drying
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a multichannel frozen cell synchronous thawing device, which comprises: a plurality of cryopreservation tubes; a thawing box, inside which a thawing cavity for accommodating a plurality of freezing storage pipes is formed; the air guide pipeline is arranged in the defrosting box, an air duct is formed in the air guide pipeline, and the outlet of the air duct faces to the plurality of defrosting cavities; the fan is arranged at the inlet of the air duct and used for conveying air flow in the air duct from the inlet to the outlet; the heating piece is arranged in the air duct and is positioned above the fan; the refrigerating piece is arranged in the air duct and is positioned below the fan; and the power output ends of the rotating mechanisms are in transmission connection with the freezing pipes and are used for driving the freezing pipes to rotate around the axes of the rotating mechanisms. During multitube resuscitates, in order to guarantee the uniformity of resuscitating the condition, through fountain formula wind channel structure, let the interior air synchronous flow of 4 cavities that unfreezes, guarantee that the interior temperature of 4 cavities is even. The independent resuscitation chamber can independently detect the real-time state of each freezing tube and carry out accurate resuscitation end point judgment.
Description
Technical Field
The invention relates to the technical field of medical experimental equipment, in particular to a multichannel frozen cell synchronous thawing device and a control method thereof.
Background
Cryopreservation of cells in suspension is a well established and accepted technique for long-term archival storage and retrieval of living cells. As a general method, cells are suspended in a cryopreservation medium that typically includes saline, buffers, nutrients, growth factors, proteins, and cryopreservation agents. The cells are then dispensed into archival storage containers of the desired size and volume, after which the temperature of the container is reduced until the container contents are frozen. Typical long-term archiving conditions include liquid nitrogen vapor storage, where temperatures are typically between-196 and-150 degrees celsius.
In order to ensure the survival rate after cell resuscitating, the requirements of different phases of different types of cells on the environmental temperature are not constant, so that the environmental temperature needs to be regulated according to the acquired tube wall temperature by a program, the cell ice crystal can quickly cross the zero point, and the overheat death of the cell after thawing caused by the overhigh environmental temperature is avoided. The resuscitation device on the market only has the heating module and does not have the refrigeration module, and is mostly solid parcel formula heating, is the temperature of the required environment of adjustment resuscitation that can not be timely.
Disclosure of Invention
The invention aims to provide a multichannel frozen cell synchronous thawing device and a control method thereof, which solve one or more of the problems in the prior art.
In one aspect, the invention provides a multi-channel frozen cell synchronous thawing device, which comprises:
a plurality of freezing tubes for storing cell fluid samples;
a thawing box, in which a plurality of thawing chambers for accommodating the freezing pipes are formed;
the air guide pipeline is arranged in the defrosting box, an air duct is formed in the air guide pipeline, and the outlet of the air duct faces to the defrosting cavities;
the fan is arranged at the inlet of the air duct and used for conveying air flow in the air duct from the inlet to the outlet;
the heating piece is arranged in the air duct and is positioned above the fan;
the refrigerating piece is arranged in the air duct and is positioned below the fan; and
the power output ends of the rotating mechanisms corresponding to the freezing pipes are in transmission connection with the freezing pipes and used for driving the freezing pipes to rotate around the axes of the rotating mechanisms.
In some embodiments, the rotating mechanism comprises a carrying platform, a rotating motor installed on the carrying platform and a freezing pipe rack arranged at the output end of the rotating motor, wherein the freezing pipe rack is used for placing the freezing pipe.
In some embodiments, the rotating mechanism further comprises a rotating bearing mounted on the carrying platform, and the rotating bearing is sleeved on the freezing pipe rack.
In some embodiments, a plurality of sample injection holes communicated with the thawing cavity are formed in the top wall of the thawing box, and the sample injection holes are in one-to-one correspondence with the cryopreservation pipes.
In some embodiments, the thawing device further comprises a plurality of lifting mechanisms corresponding to the thawing pipes, wherein power output ends of the lifting mechanisms are in transmission connection with the thawing pipes and used for driving the thawing pipes to lift and enter and exit the thawing cavity through the sample injection holes.
In some embodiments, the lifting mechanism comprises a lifting guide rail, a lifting screw and a lifting driving motor, wherein the lifting guide rail penetrates through the carrying platform, the lifting driving motor is in transmission fit with the lifting screw, and the lifting screw penetrates through the carrying platform and is meshed with the carrying platform.
In some embodiments, the heating element is composed of a plurality of annular heating tubes arranged side by side, and the cooling element comprises a cooling guide block, a semiconductor cooling fin and a cooling fin which are arranged from top to bottom.
In some embodiments, the thawing device further comprises a first infrared temperature sensor and a proximity switch for monitoring the cryopreservation tube, and the thawing device is provided with a controller, wherein the controller is electrically connected with the first infrared temperature sensor, the proximity switch and the lifting mechanism, so that the controller can receive signals acquired by the first infrared temperature sensor and the proximity switch and control the lifting mechanism.
In some embodiments, the thawing device further comprises a second infrared temperature sensor for monitoring the thawing tube and an NTC temperature probe for monitoring the temperature in the thawing cavity, and the thawing device is provided with a controller, wherein the controller, the second infrared temperature sensor, the NTC temperature probe, the heating element and the refrigerating element are arranged in the thawing device, so that the controller can receive signals acquired by the second infrared temperature sensor and control working parameters of the heating element and the refrigerating element.
On the other hand, the control method of the multichannel frozen cell synchronous thawing device is characterized by comprising the following steps of: the control method is applied to a channel frozen cell synchronous thawing device and comprises the following steps:
s1, starting equipment, starting a heating element and a fan, and entering a preheating process;
s2, measuring the temperature of the defrosting cavity by the NTC temperature probe to reach a preset temperature, and finishing preheating;
s3, placing the frozen storage tube into a frozen storage tube rack, detecting whether the temperature of the frozen storage tube is lower than a preset value or not by using a first infrared temperature sensor, if yes, directly entering S4, otherwise, prompting by using equipment to alarm, and not resuscitating;
s4, starting to operate the lifting driving motor, and driving the freezing tube to enter the thawing cavity by the objective table;
s5, starting to operate the rotating motor to drive the objective table to rotate, starting a second infrared temperature sensor to measure the surface temperature of the freezing tube in real time, starting an NTC temperature probe to measure the temperature in the defrosting cavity in real time, and enabling the equipment to enter a defrosting process;
s6, controlling the heating element and the refrigerating element to control the temperature in the defrosting cavity in real time through a PID control module of the controller;
and S7, after thawing is completed, the object stage moves the freezing tube to return to the initial position, and the system prompts that recovery is completed and the freezing tube is taken out.
The beneficial effects are that:
1. when the thawing device provided by the invention is used for multitube resuscitation, in order to ensure consistency of resuscitation conditions, air in 4 thawing cavities synchronously flows through a fountain type air duct structure, and the temperature uniformity in the 4 cavities is ensured. The independent resuscitation chamber can independently detect the real-time state of each freezing tube and carry out accurate resuscitation end point judgment.
2. The thawing device is provided with the integrated fountain type annular air duct, the heating and refrigerating module is arranged in the air duct, and the method of wrapping and surrounding the heating cryopreservation tube by air is adopted, so that the temperature of a resuscitating environment can be timely and rapidly controlled, the resuscitating environment is more suitable for the temperature required by cells, and the survival rate of cell resuscitating is improved.
Drawings
FIG. 1 is a bottom view of a multi-channel synchronized thawing apparatus for frozen cells according to an embodiment of the present invention;
FIG. 2 is a schematic top view of a multi-channel synchronous thawing apparatus for frozen cells according to an embodiment of the present invention;
FIG. 3 is a cross-sectional view of a multi-channel synchronized thawing apparatus for frozen cells according to an embodiment of the present invention;
fig. 4 is a control flow chart of a multi-channel synchronous thawing device for frozen cells according to an embodiment of the invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
As shown in fig. 1 and 2:
a multi-channel frozen cell synchronous thawing device, comprising:
four cryopreservation tubes 10 for storing a cell fluid sample;
a thawing box, in which four thawing chambers 21 for accommodating the freezing storage tube 10 are formed;
the air guide pipeline 30 is arranged in the defrosting box, an air channel is formed in the air guide pipeline, and the outlet of the air channel faces four defrosting cavities 2;
a fan 40, disposed at the inlet of the air duct, for conveying the air flow in the air duct from the inlet to the outlet;
the heating element 50 is arranged in the air duct and is positioned above the fan, and the heating element 50 consists of a plurality of annular heating pipes which are arranged side by side;
the refrigerating piece 60 is arranged in the air duct and is positioned below the fan, and the refrigerating piece 60 comprises a cold guide block, a semiconductor refrigerating sheet and a radiating sheet which are arranged from top to bottom;
four freezes deposits the pipe 10 and is first freezes deposits pipe 101, second freezes deposits pipe 102, third freezes deposits pipe 103 and fourth freezes deposits pipe 104 respectively, and this four freezes deposits the pipe and distributes clockwise in proper order around fan 40, through this fountain wind channel structure, lets four indoor air synchronous flow that unfreezes, guarantees four indoor temperature uniformity, independent resuscitates the cavity, can independently detect the real-time state of every freezes deposits the pipe, carries out accurate resuscitates terminal point judgement.
The fountain type air duct structure designs the heating and refrigerating module, adopts the method of wrapping and encircling the heating freezing tube by air, can timely and rapidly control the resuscitating ambient temperature, ensures that the resuscitating environment is more suitable for the temperature required by cells, and improves the survival rate of cell resuscitating.
As shown in fig. 3:
a multi-channel frozen cell synchronous thawing device, comprising:
a plurality of freezing tubes 10 for storing a cell fluid sample;
the thawing box is internally provided with a plurality of thawing cavities 21 for accommodating the cryopreservation pipes 10, the top wall of the thawing box is provided with a plurality of sample injection holes 11 communicated with the thawing cavities 21, and the sample injection holes 11 are in one-to-one correspondence with the cryopreservation pipes 10;
an air guide duct 30 provided in the defrosting box, and having an air duct formed therein, the air duct having an outlet directed toward the plurality of defrosting chambers 21;
a fan 40, disposed at the inlet of the air duct, for conveying the air flow in the air duct from the inlet to the outlet;
the heating element 50 is arranged in the air duct and is positioned above the fan, and the heating element 50 consists of a plurality of annular heating pipes which are arranged side by side;
the refrigerating piece 60 is arranged in the air duct and is positioned below the fan, and the refrigerating piece 60 comprises a cold guide block, a semiconductor refrigerating sheet and a radiating sheet which are arranged from top to bottom;
the power output ends of the plurality of rotating mechanisms corresponding to the freezing pipes are in transmission connection with the freezing pipes 10 and are used for driving the freezing pipes 10 to rotate around the axes of the rotating mechanisms, the rotating mechanisms comprise a carrying platform 71, a rotating motor 72 arranged on the carrying platform 71, a freezing pipe rack 73 arranged at the output end of the rotating motor 72 and a rotating bearing 74 arranged on the carrying platform 71, the freezing pipe rack 73 is used for placing the freezing pipes 10, and the rotating bearing 74 is sleeved on the freezing pipe rack 73;
the lifting mechanisms are corresponding to the freezing pipes 10, the power output ends of the lifting mechanisms are in transmission connection with the freezing pipes 10 and used for driving the freezing pipes 10 to lift and enter and exit the thawing cavity 21 through the sample injection holes 11, the lifting mechanisms comprise lifting guide rails 81, lifting screws and lifting driving motors 82, the lifting guide rails 81 penetrate through the carrying platform 71, the lifting driving motors 82 are in transmission fit with the lifting screws, and the lifting screws penetrate through the carrying platform 71 and are meshed with the carrying platform 71.
In order to realize intelligent control, the thawing device further comprises a first infrared temperature sensor and a proximity switch for monitoring the cryopreservation tube 10, wherein the thawing device is provided with a controller, and the controller is electrically connected with the first infrared temperature sensor, the proximity switch and the lifting mechanism, so that the controller can receive signals acquired by the first infrared temperature sensor and the proximity switch and control the lifting mechanism; meanwhile, the refrigerator further comprises a second infrared temperature sensor for monitoring the freezing tube and an NTC temperature probe for monitoring the temperature in the thawing cavity, and the controller is further connected with the second infrared temperature sensor, the NTC temperature probe, the heating element 50 and the refrigerating element 60, so that the controller can receive signals acquired by the second infrared temperature sensor and control working parameters of the heating element 50 and the refrigerating element 60.
The control method based on the thawing device, as shown in fig. 4, comprises the following steps:
s1, starting equipment, starting a heating element and a fan, and entering a preheating process;
s2, measuring the temperature of the defrosting cavity by the NTC temperature probe to reach a preset temperature, and finishing preheating;
s3, placing the frozen storage tube into a frozen storage tube rack, detecting whether the temperature of the frozen storage tube is lower than a preset value or not by using a first infrared temperature sensor, if yes, directly entering S4, otherwise, prompting by using equipment to alarm, and not resuscitating;
s4, starting to operate the lifting driving motor, and driving the freezing tube to enter the thawing cavity by the objective table;
s5, starting to operate the rotating motor to drive the objective table to rotate, starting a second infrared temperature sensor to measure the surface temperature of the freezing tube in real time, starting an NTC temperature probe to measure the temperature in the defrosting cavity in real time, and enabling the equipment to enter a defrosting process;
s6, controlling the heating element and the refrigerating element to control the temperature in the defrosting cavity in real time through a PID control module of the controller;
and S7, after thawing is completed, the object stage moves the freezing tube to return to the initial position, and the system prompts that recovery is completed and the freezing tube is taken out.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that a person skilled in the art may make several similar variations and modifications without departing from the inventive concept, which should also be considered as being within the scope of the present invention.
Claims (10)
1. A multichannel frozen cell synchronous thawing device, which is characterized by comprising:
a plurality of cryopreservation tubes (10) for storing a cell fluid sample;
a thawing box, in which a plurality of thawing chambers (21) for accommodating the freezing storage tube (10) are formed;
an air guide pipeline (30) which is arranged in the defrosting box, wherein an air channel is formed in the defrosting box, and the outlet of the air channel faces to a plurality of defrosting cavities (21);
a fan (40) arranged at the inlet of the air duct and used for conveying air flow in the air duct from the inlet to the outlet;
the heating piece (50) is arranged in the air duct and is positioned above the fan;
a refrigerating piece (60) which is arranged in the air duct and is positioned below the fan; and
the power output ends of the rotating mechanisms corresponding to the freezing pipes are in transmission connection with the freezing pipes (10) and are used for driving the freezing pipes (10) to rotate around the axes of the rotating mechanisms.
2. The multi-channel frozen cell synchronous thawing device according to claim 1, wherein the rotating mechanism comprises a carrying platform (71), a rotating motor (72) arranged on the carrying platform (71) and a frozen tube rack (73) arranged at the output end of the rotating motor (72), and the frozen tube rack (73) is used for placing the frozen tube (10).
3. The multi-channel frozen cell synchronous thawing device according to claim 2, wherein the rotating mechanism further comprises a rotating bearing (74) arranged on the carrying platform (71), and the rotating bearing (74) is sleeved on the frozen tube rack (73).
4. A multi-channel frozen cell synchronous thawing device according to claim 3, characterized in that a plurality of sample injection holes (11) communicated with the thawing cavity (21) are formed in the top wall of the thawing box, and the sample injection holes (11) are in one-to-one correspondence with the frozen storage tubes (10).
5. The multi-channel frozen cell synchronous thawing device according to claim 4, further comprising a plurality of lifting mechanisms corresponding to the frozen storage tubes (10), wherein the power output ends of the lifting mechanisms are in transmission connection with the frozen storage tubes (10) and are used for driving the frozen storage tubes (10) to lift and enter and exit the thawing cavity (21) through the sample injection holes (11).
6. The multi-channel frozen cell synchronous thawing device according to claim 5, wherein the lifting mechanism comprises a lifting guide rail (81), a lifting screw and a lifting driving motor (82), the lifting guide rail (81) is arranged on the carrying platform (71) in a penetrating manner, the lifting driving motor (82) is in transmission fit with the lifting screw, and the lifting screw is arranged on the carrying platform (71) in a penetrating manner and meshed with the carrying platform (71).
7. The multi-channel frozen cell synchronous thawing device according to claim 1, wherein the heating element (50) is composed of a plurality of annular heating pipes arranged side by side, and the refrigerating element (60) comprises a cold guide block, a semiconductor refrigerating plate and a radiating plate which are arranged from top to bottom.
8. The multi-channel synchronous thawing device for frozen cells according to claim 1, further comprising a first infrared temperature sensor and a proximity switch for monitoring the frozen tube (10), wherein the thawing device is provided with a controller, and the controller is electrically connected with the first infrared temperature sensor, the proximity switch and the lifting mechanism, so that the controller can receive signals collected by the first infrared temperature sensor and the proximity switch and control the lifting mechanism.
9. The multi-channel frozen cell synchronous thawing device according to claim 1, further comprising a second infrared temperature sensor for monitoring the frozen tube and an NTC temperature probe for monitoring the temperature in the thawing chamber, wherein the thawing device is provided with a controller, and the controller, the second infrared temperature sensor, the NTC temperature probe, the heating element (50) and the refrigerating element (60) are arranged so that the controller can receive signals acquired by the second infrared temperature sensor and control the working parameters of the heating element (50) and the refrigerating element (60).
10. A control method of a multichannel frozen cell synchronous thawing device is characterized by comprising the following steps of: the control method is applied to the multichannel frozen cell synchronous thawing device as claimed in any one of claims 1 to 9, and comprises the following steps:
s1, starting equipment, starting a heating element and a fan, and entering a preheating process;
s2, measuring the temperature of the defrosting cavity by the NTC temperature probe to reach a preset temperature, and finishing preheating;
s3, placing the frozen storage tube into a frozen storage tube rack, detecting whether the temperature of the frozen storage tube is lower than a preset value or not by using a first infrared temperature sensor, if yes, directly entering S4, otherwise, prompting by using equipment to alarm, and not resuscitating;
s4, starting to operate the lifting driving motor, and driving the freezing tube to enter the thawing cavity by the objective table;
s5, starting to operate the rotating motor to drive the objective table to rotate, starting a second infrared temperature sensor to measure the surface temperature of the freezing tube in real time, starting an NTC temperature probe to measure the temperature in the defrosting cavity in real time, and enabling the equipment to enter a defrosting process;
s6, controlling the heating element and the refrigerating element to control the temperature in the defrosting cavity in real time through a PID control module of the controller;
and S7, after thawing is completed, the object stage moves the freezing tube to return to the initial position, and the system prompts that recovery is completed and the freezing tube is taken out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202310918192.3A CN117143722B (en) | 2023-07-25 | 2023-07-25 | Multichannel frozen cell synchronous thawing device and control method thereof |
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| CN202310918192.3A CN117143722B (en) | 2023-07-25 | 2023-07-25 | Multichannel frozen cell synchronous thawing device and control method thereof |
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| CN117143722B CN117143722B (en) | 2024-07-09 |
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| DE4101926A1 (en) * | 1991-01-23 | 1992-07-30 | Simmet Ludwig Dr Med Vet | Device for thawing deep-frozen living cells in test-tubes - test-tubes are placed in flow of warm water in standing pipe above water tank so that water returns to tank as soon as pump is stopped |
| US20040006999A1 (en) * | 2001-11-01 | 2004-01-15 | Integrated Biosystems, Inc. | Systems and methods for freezing, mixing and thawing biopharmacuetical material |
| US20140251584A1 (en) * | 2013-03-11 | 2014-09-11 | Air Bath Technologies, LLC | System For Precision Temperature Control of Thermal Bead Baths |
| CN105465928A (en) * | 2015-12-15 | 2016-04-06 | 西安工程大学 | Fountain heat radiating and mechanical refrigeration combined cold supply system for data center |
| CN205794726U (en) * | 2016-05-18 | 2016-12-14 | 北京百年盛隆厨房设备有限公司 | A kind of defrosting pond |
| US20190308194A1 (en) * | 2016-07-14 | 2019-10-10 | Hitachi High-Technologies Corporation | Automatic Analyzer |
| CN108260587A (en) * | 2018-01-15 | 2018-07-10 | 赛慕特生物工程（上海）有限公司 | It a kind of large capacity cell cryopreservation tube and its mating freezes and dry type recovery device |
| CN110093252A (en) * | 2019-05-29 | 2019-08-06 | 赛慕特生物工程（上海）有限公司 | A kind of universal cell cryopreservation product dry type resuscitation system and working method |
| CN110305786A (en) * | 2019-06-20 | 2019-10-08 | 大连理工大学 | A kind of hand-held, can be from the dry type cell resuscitator of disinfection |
| CN212748497U (en) * | 2020-07-02 | 2021-03-19 | 杭州欧飞食品有限公司 | A thawing apparatus for food detection |
| CN116240106A (en) * | 2023-01-18 | 2023-06-09 | 上海萌薇生物医疗科技有限公司 | Cell resuscitation system and control method thereof |
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