CN117126736A - Biological culture device for enzyme conversion - Google Patents

Biological culture device for enzyme conversion Download PDF

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Publication number
CN117126736A
CN117126736A CN202311401013.5A CN202311401013A CN117126736A CN 117126736 A CN117126736 A CN 117126736A CN 202311401013 A CN202311401013 A CN 202311401013A CN 117126736 A CN117126736 A CN 117126736A
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Prior art keywords
fixedly connected
wall
culture
enzyme
pipe
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CN202311401013.5A
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Inventor
李广群
鞠瑞成
王燕蓬
郑超
王晓霞
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Shandong Baiwo Biotechnology Co ltd
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Shandong Baiwo Biotechnology Co ltd
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Priority to CN202311401013.5A priority Critical patent/CN117126736A/en
Publication of CN117126736A publication Critical patent/CN117126736A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/52—Mobile; Means for transporting the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

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  • Health & Medical Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a biological culture device for enzyme conversion, which relates to the technical field of culture devices and comprises a support control mechanism, wherein the top of the support control mechanism is fixedly connected with a culture mechanism, the culture mechanism comprises a base, and the bottom of the inner wall of the base is provided with a fixed groove. In use, the liquid metal is used as a heat conducting medium under the action of the culture mechanism, when the culture device is required to be subjected to heating treatment, the heating pipe can start heating, heat can be conveyed into the culture dish under the transmission of the inner pipe, the liquid metal and the shell guide pipe, after the heating is finished, the high-temperature liquid metal is replaced by the normal-temperature liquid metal, the transmission of temperature is blocked, the cooling speed is high, the enzyme conversion efficiency is high, the liquid metal can be cooled under the cooperation of the cooling device, the heat and cold replacement of the internal temperature can be realized, the temperature adjustment can be finished in real time, and the practicability of equipment is improved.

Description

Biological culture device for enzyme conversion
Technical Field
The invention relates to the technical field of culture devices, in particular to a biological culture device for enzyme conversion.
Background
The enzyme reactor is a device for catalyzing conversion reaction of biological enzyme, which provides a proper environment to make enzyme catalyze reaction under the best condition, and is generally composed of reaction container, temperature control device, stirring device, substrate supply device and product collecting device, etc., and the principle of the enzyme reactor is that the enzyme and substrate react specifically to produce required product under the condition of a certain reaction temperature and pH, and the enzyme reactor can control the parameters of concentration, reaction time, temperature and pH, etc. of the substrate to optimize reaction condition, raise yield and purity of the product, and the enzyme reactor is widely used in various fields such as food industry, pharmaceutical industry, biofuel production and biochemical synthesis, etc. and has the characteristics of high efficiency, environmental protection and repeated use, etc., so that it is widely focused and applied.
In the prior art, for example, chinese patent numbers: CN213232303U provides a biological enzyme culture apparatus, including organism, host computer and liquid reserve tank, organism one side is through bolted connection have the host computer, the organism upper end middle part is through bolted connection have the liquid reserve tank, liquid reserve tank one side is through water piping connection there is the measuring pump, be connected with the spray tube bank through the couple in the organism, the spray tube bank with the measuring pump passes through the ferrule and is connected. The beneficial effects are that: through setting up liquid reserve tank, measuring pump and spray tube bank, can take out the culture solution in the liquid reserve tank through the measuring pump to in carrying the spray tube bank, evenly spray on the culture dish through the spray tube bank, can accurate control the additive amount of culture solution, thereby replace manual work to add, easy operation is convenient, labour saving and time saving, efficient, through setting up supporting shoe, fixing bolt, can guarantee that the culture dish is placed stably, fixed effectual, effectively reduces the rocking of culture dish, thereby improves biological enzyme's survival rate and culture quality.
Above-mentioned equipment is nevertheless can effectually reduce equipment and cultivate rocking that the in-process produced at carrying out biological enzyme to improve biological enzyme survival rate and quality, but current enzymatic conversion device heating method is more, and after heating its inside raw materials, need carry out cooling treatment to it afterwards, and current cooling method is through natural cooling, and cooling rate is slower, and the time that needs wait is very long, leads to enzymatic conversion inefficiency, and when its inside needs to realize cold and hot alternating treatment, current equipment is difficult to realize, causes equipment to have the restriction when using.
Disclosure of Invention
The invention aims to provide an enzyme-converted biological culture device, which aims to solve the problems that the cooling time is long, the production efficiency is low, cold and cold alternation cannot be completed, and the limitation exists in the use of equipment because the equipment cannot be subjected to effective cooling treatment in the background.
In order to achieve the above purpose, the present invention provides the following technical solutions: the biological culture device for enzyme conversion comprises a support control mechanism, wherein the top of the support control mechanism is fixedly connected with a culture mechanism;
the culture mechanism comprises a base, the fixed slot has been seted up to the inner wall bottom of base, fixedly inserted between the interior table wall of fixed slot has the shell pipe, and the outward appearance wall activity of shell pipe runs through the inside of base, the interior table wall fixedly connected with multiunit interconnect board of shell pipe, multiunit fixedly connected with inner tube between the interior table wall of interconnect board, the interior table wall fixedly connected with heating pipe of inner tube, the shell pipe with be provided with the inside groove between outer wall one side of inner tube, the shell pipe with the outer wall one side of inner tube is fixed to be linked together and is had two ooff valves A, and two ooff valve A's input and output are all linked together with the inside groove, two one of them output fixed communication of ooff valve A has pipe A, pipe A's output fixed communication has the bin, the top fixed communication of bin has the micropump, the output fixed communication of micropump has pipe B, and the output of pipe B and two ooff valve A's another input fixed communication.
Preferably, the bottom fixedly connected with mounting panel of bin, the fixed transportation ring pipe that inserts in inner wall bottom of base, transportation ring pipe's bottom fixed communication has ooff valve B, ooff valve B's output fixed communication has the output tube, transportation ring pipe's top fixed communication has the multiunit leak hole, first through installing the bin at the top of mounting panel, be connected the inside of mounting panel and support control mechanism, can keep the steady state of bin, and transportation ring pipe's exterior wall is the inside that sets up at the culture dish, after its inside cultivates biological enzyme, thereby can realize the intercommunication between output tube and the transportation ring pipe through starting ooff valve B, finally invertase permeates into the inside of multiunit leak hole, and regard output tube and transportation ring pipe as the transportation passageway to carry it out.
Preferably, the inner surface wall of the storage box is fixedly connected with a temperature guide plate, the bottom of the storage box is fixedly connected with a compressor, the output end of the compressor is fixedly communicated with a condenser pipe, the outer surface wall of the condenser pipe is contacted with the bottom of the inner wall of the storage box, the output end of the condenser pipe is fixedly communicated with a capillary pipe, the output end of the capillary pipe is fixedly communicated with an evaporator, the outer surface wall of the evaporator is contacted with the bottom of the temperature guide plate, the temperature guide plate is firstly arranged in the storage box, two end spaces can be formed in the storage box, when the liquid in the storage box needs to be cooled, the compressor is started, the low-temperature low-pressure gaseous condensing agent is sucked in, and under the compression of the compressor, the high-temperature high-pressure gaseous condensing agent is kept to form high-temperature gaseous, and high-pressure gaseous is introduced into the condenser pipe to cool and dissipate heat, part of gaseous entering into the condenser pipe can become medium-temperature liquid after being condensed, at the moment, the medium-temperature liquid can enter the inside the capillary pipe, and the capillary pipe because the capillary pipe is suddenly narrowed at the position, the pressure drop is caused, the pressure drop is realized, the pressure drop part is suddenly evaporated, the liquid can suddenly and the evaporator is cooled down, the top of the evaporator can be cooled down, the outer surface of the liquid can be cooled down by the evaporator, and the top of the evaporator can be cooled down, and the outer surface of the evaporator can be cooled down, and the liquid can be cooled down by the evaporator, and the outer surface can be cooled down.
Preferably, the culture dish is inserted to the interior table wall activity of base, the exterior wall of culture dish is provided with the observation port, there is sealed lid at the top of culture dish through the hinge upset, the top fixedly connected with driving motor of sealed lid, and driving motor's rotating end activity runs through in the inside of sealed lid, the top fixed intercommunication of sealed lid has the inlet pipe, the interior table wall activity of inlet pipe is inlayed and is equipped with the sealing plug, is provided with the observation port at the exterior wall of culture dish at first, and self intensity is higher, high temperature resistant and have the transparency, and the staff of being convenient for observe the invertase of its inside handles, and can add the raw materials through the inlet pipe to its inside and handle, after the raw materials is added, inserts the sealing plug activity and establishes in the inside of inlet pipe to can keep the sealing state of equipment, carry out enzyme conversion for the biology and provide good environment.
Preferably, the outer surface wall fixedly connected with two mounting brackets of culture dish, the interior surface wall fixedly connected with fixed disk of culture dish, the bottom of fixed disk has been seted up and has been driven the commentaries on classics groove, movable groove A has been seted up to the top center department of fixed disk, a plurality of movable grooves B have been seted up at the top of fixed disk, at first movable groove A and the movable groove B of seting up at the top of fixed disk, are convenient for drive bull stick and gangbar and implement the rotation processing in its inside, it is convenient to improve for subsequent work operation.
Preferably, the inner surface wall of the movable groove A is movably inserted with a driving rod, the rotating end of the driving motor is fixedly connected with the top of the driving rod, a plurality of groups of stirring blades are fixedly sleeved on the outer surface wall of the driving rod, the driving motor can drive the driving rod to implement rotation treatment at first under the electrified state, the driving rod and the plurality of groups of stirring blades are in a fixed connection state, under the running state, the linkage rotation state of the driving rod and the plurality of groups of stirring blades can be kept, so that the stirring treatment of raw materials in the driving rod can be accelerated, and the fusion property between raw materials is improved.
Preferably, the outer surface wall of the driving rod is fixedly connected with a gear A, the outer surface wall of the gear A is in meshed connection with a plurality of gears B, a plurality of gears B are fixedly connected with a linkage rod at the top of the gear B, the outer surface walls of the plurality of linkage rods are movably inserted into the movable groove B, the inner surface wall of the driving groove is movably embedded with a driving block, the bottom of the driving block is fixedly connected with a movable disc, the inner surface wall of the movable disc is in meshed connection with the outer surface walls of the plurality of gears B, the bottom of the movable disc is fixedly connected with three scraping plates, the outer surface walls of the three scraping plates are in contact with the inner surface wall of the culture dish, the gear A is fixedly sleeved between the outer surface walls of the driving rod, when a driving motor drives the driving rod to rotate, the linkage rod A can be kept in linkage rotation, and meshed rotation is kept between the gear A and the plurality of gears B, the tops of the gears B are respectively fixed with the linkage rod, the linkage rod is rotated in the movable groove B, the movable disc is meshed with the movable disc, the driving point B is provided for the gear B, and the inner surface of the three scraping plates can be meshed with the inner surface plates through the three scraping plates, and the inner surfaces of the rotating plates can be mutually meshed with each other, and the inner surfaces of the rotating plates can be conveniently fused.
Preferably, the support control mechanism comprises a fixing frame, four bottom wheels are arranged at the bottom of the fixing frame, a control box is fixedly connected to the top of the fixing frame, a control panel is arranged at the top of the control box, four bottom wheels are arranged at the bottom of the device, when the bottom wheels rotate and are in contact with the ground, so that effective position movement treatment can be performed on equipment, workers can convey the designation through the control panel, and finally the designation is transmitted to the position of each electric equipment by the control box to perform corresponding control treatment.
Preferably, the output end of the control box is fixedly communicated with a wire, the output end of the wire is fixedly communicated with a plurality of electromagnetic valves, the output ends of the electromagnetic valves are fixedly communicated with a conveying pipe, the bottoms of the electromagnetic valves are fixedly connected with the bottoms of the fixing frame, at first, an external conveying raw material pipeline is respectively communicated with the electromagnetic valves, the control box is used for effectively controlling the communication of the conveying channel, and proper oxygen, pH value and the like can be provided for the inside of the equipment through the electromagnetic valves, so that the biological enzyme in the equipment can be effectively kept to react fully.
Preferably, a plurality of output of conveyer pipe all is linked together with the inside of culture dish, the outer wall one side of mounting panel is linked together through inside line group with the outer wall one side fixed connection of mount, the input of compressor and the output of control box, two the outer wall one side of mounting bracket all is linked together with the outer wall one side fixed connection of mount, at first with the outer wall one side of mounting panel and the outer wall one side fixed connection of mount, can accomplish the fixed connection state between support control mechanism and the culture mechanism both, keep its stability when the operation.
Compared with the prior art, the invention has the beneficial effects that:
in use, the liquid metal is used as a heat conducting medium under the action of the culture mechanism, when the culture device is required to be subjected to heating treatment, the heating pipe can start heating, heat can be conveyed into the culture dish under the transmission of the inner pipe, the liquid metal and the shell guide pipe, after the heating is finished, the high-temperature liquid metal is replaced by the normal-temperature liquid metal, the transmission of temperature is blocked, the cooling speed is high, the enzyme conversion efficiency is high, the liquid metal can be cooled under the cooperation of the cooling device, the heat and cold replacement of the internal temperature can be realized, the temperature adjustment can be finished in real time, and the practicability of equipment is improved.
In use, under the action of the culture mechanism, when the equipment effectively stirs the raw materials in the culture dish, the scraping treatment can be reversely carried out on the raw materials adhered to the surface wall in the culture dish, so that the raw materials are separated from the culture dish, the raw materials can be fused with each other, the problem of insufficient raw materials caused by adhesion is avoided, the enzyme conversion quality is improved, and the later cleaning is also facilitated.
According to the invention, under the action of the support control mechanism and the culture mechanism, the oxygen, the pH value and the like can be added into the device through detection, so that the reaction rate is further improved, the rapid conversion of biological enzymes is facilitated, and the device is high in automatic detection and addition efficiency.
Drawings
FIG. 1 is a perspective view showing the structure of an enzyme-transformed biological culture device according to the present invention;
FIG. 2 is a side view in perspective of an enzyme-converted biological growth device of the present invention;
FIG. 3 is a perspective view of a support control mechanism in an enzyme-converted biological growth device of the present invention;
FIG. 4 is a plan view of a culture mechanism in an enzyme-converted biological culture device of the invention;
FIG. 5 is a partially exploded perspective view of a culture mechanism in an enzyme-converted biological culture device of the present invention;
FIG. 6 is a plan sectional view showing the internal structure of a housing tube in an enzyme-converted biological culture device according to the present invention;
FIG. 7 is a plan view of a cooling assembly of a culture mechanism in an enzyme-converted biological culture device according to the invention;
FIG. 8 is a perspective exploded view of a culture dish in an enzyme-transformed biological culture device of the present invention;
FIG. 9 is a perspective exploded view of the inside of a culture mechanism in an enzyme-converted biological culture device of the present invention;
FIG. 10 is a plan view of a portion of a gear A in an enzyme-converted biological growth device of the present invention.
In the figure: 1. a support control mechanism; 101. a fixing frame; 102. a bottom wheel; 103. a control box; 104. a control panel; 106. a wire; 107. an electromagnetic valve; 108. a delivery tube; 2. a culturing mechanism; 201. a base; 202. a fixing groove; 203. a housing conduit; 204. connecting rods internally; 205. an inner tube; 206. heating pipes; 207. an inner tank; 208. a switch valve A; 209. a conduit A; 210. a storage tank; 211. a micropump; 212. a conduit B; 213. a mounting plate; 214. a conveying annular pipe; 215. a switch valve B; 216. a leak hole; 217. a temperature guide plate; 218. a compressor; 219. a condensing tube; 220. a capillary tube; 221. an evaporator; 222. a culture dish; 223. an observation port; 224. sealing cover; 225. a driving motor; 226. a feed pipe; 227. a sealing plug; 228. a mounting frame; 229. a fixed plate; 230. a driving groove; 231. a movable groove A; 232. a movable groove B; 233. a driving rod; 234. stirring blades; 235. a gear A; 236. a gear B; 237. a linkage rod; 238. a driving block; 239. a movable plate; 240. a scraper; 241. and outputting the pipe.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-10, the present invention provides an enzyme-transformed biological culture apparatus, which comprises a support control mechanism 1, wherein a culture mechanism 2 is fixedly connected to the top of the support control mechanism 1;
the culture mechanism 2 comprises a base 201, a fixing groove 202 is formed in the bottom of the inner wall of the base 201, a shell conduit 203 is fixedly inserted between the inner surface walls of the fixing groove 202, the outer surface walls of the shell conduit 203 movably penetrate through the inside of the base 201, a plurality of groups of inner connecting rods 204 are fixedly connected to the inner surface walls of the shell conduit 203, an inner pipe 205 is fixedly connected between the inner surface walls of the plurality of groups of inner connecting rods 204, a heating pipe 206 is fixedly connected to the inner surface walls of the inner pipe 205, an inner groove 207 is formed between the shell conduit 203 and one side of the outer wall of the inner pipe 205, the shell conduit 203 is fixedly communicated with one side of the outer wall of the inner pipe 205, two switch valves A208, the input ends and the output ends of the two switch valves A208 are all communicated with the inner groove 207, one output end of the two switch valves A208 is fixedly communicated with a conduit A209, the output end of the conduit A209 is fixedly communicated with a storage box 210, the top of the storage box 210 is fixedly communicated with a micro pump 211, the output end of the micro pump 211 is fixedly communicated with a conduit B212, and the output end of the conduit B212 is fixedly communicated with the other input end of the two switch valves A208.
According to fig. 3 and 4-5: the bottom fixedly connected with mounting panel 213 of bin 210, the fixed transport ring pipe 214 that inserts in the inner wall bottom of base 201, the fixed intercommunication in bottom of transport ring pipe 214 has ooff valve B215, the fixed intercommunication in output of ooff valve B215 has output tube 241, the fixed intercommunication in top of transport ring pipe 214 has multiunit leak hole 216, firstly, through installing bin 210 at the top of mounting panel 213, be connected the inside of mounting panel 213 and support control mechanism 1, can keep the steady state of bin 210, and the outward appearance wall of transport ring pipe 214 is the inside that sets up at culture dish 222, after its inside is to bio-enzyme cultivation, can thereby realize the intercommunication between output tube 241 and the transport ring pipe 214 through starting ooff valve B215, finally, invertase permeates into the inside of multiunit leak hole 216, and take output tube 241 and transport ring pipe 214 as the delivery channel and carry it out.
According to fig. 4-5 and 7: the inner surface wall of the storage tank 210 is fixedly connected with the temperature guide plate 217, the bottom of the storage tank 210 is fixedly connected with the compressor 218, the output end of the compressor 218 is fixedly communicated with the condenser pipe 219, the outer surface wall of the condenser pipe 219 is contacted with the bottom of the inner wall of the storage tank 210, the output end of the condenser pipe 219 is fixedly communicated with the capillary 220, the output end of the capillary 220 is fixedly communicated with the evaporator 221, the outer surface wall of the evaporator 221 is contacted with the bottom of the temperature guide plate 217, the temperature guide plate 217 is firstly arranged in the storage tank 210, two end spaces can be formed in the storage tank 210, when the liquid in the storage tank 210 needs to be cooled, the compressor 218 is started, the compressor 218 sucks low-temperature low-pressure gaseous condensing agent, and can keep the condensing agent to form high-temperature high-pressure gas under the compression of the self, the high-temperature high-pressure gas enters the condenser pipe 219 for cooling and radiating, part of the gas entering the condenser pipe 219 can become medium-temperature liquid after condensation, at this time, the medium-temperature liquid can enter the capillary 220, the capillary 221 can be firstly, the capillary 221 can be arranged at the position, the evaporator 221 can be suddenly cooled down, the evaporator 221 can suddenly, the temperature can be suddenly cooled down, the top of the liquid can be suddenly cooled down, and the evaporator can be suddenly cooled down, the evaporator 221 can be suddenly cooled down, and the evaporator can be suddenly cooled down, the top part can be suddenly cooled down, and the evaporator 221, and the top can be suddenly cooled down, and the top can be cooled down, and the evaporator can be suddenly cooled down.
According to fig. 6-8: the culture dish 222 is movably inserted into the inner surface wall of the base 201, an observation port 223 is formed in the outer surface wall of the culture dish 222, a sealing cover 224 is turned over by a hinge at the top of the culture dish 222, a driving motor 225 is fixedly connected to the top of the sealing cover 224, the rotating end of the driving motor 225 movably penetrates through the inside of the sealing cover 224, a feeding pipe 226 is fixedly communicated with the top of the sealing cover 224, a sealing plug 227 is movably embedded into the inner surface wall of the feeding pipe 226, the observation port 223 is formed in the outer surface wall of the culture dish 222, the strength of the culture dish is high, the culture dish is high in temperature resistance and transparent, workers can observe and process invertase in the culture dish conveniently, raw materials can be added into the culture dish through the feeding pipe 226, after raw materials are added, the sealing plug 227 is movably inserted into the inside of the feeding pipe 226, so that the sealing state of equipment can be maintained, and a good environment is provided for enzyme conversion for the living things.
According to the fig. 8-10: the outer surface wall fixedly connected with two mounting brackets 228 of culture dish 222, the interior surface wall fixedly connected with fixed disk 229 of culture dish 222, drive changeing groove 230 has been seted up to the bottom of fixed disk 229, movable groove A231 has been seted up to the top center department of fixed disk 229, a plurality of movable grooves B232 have been seted up at the top of fixed disk 229, at first movable groove A231 and movable groove B232 of seting up at the top of fixed disk 229, it is convenient for drive bull stick 233 and gangbar 237 to implement the rotation processing in its inside, it is convenient to improve for subsequent work operation.
According to fig. 9: the inner surface wall of the movable groove A231 is movably inserted with a driving rod 233, the rotating end of the driving motor 225 is fixedly connected with the top of the driving rod 233, a plurality of groups of stirring blades 234 are fixedly sleeved on the outer surface wall of the driving rod 233, the driving motor 225 can drive the driving rod 233 to implement rotation treatment in the electrified state, the driving rod 233 and the plurality of groups of stirring blades 234 are in a fixed connection state, and in the running state, the linkage rotation state of the driving rod 233 and the plurality of groups of stirring blades 234 can be kept, so that the stirring treatment of raw materials in the driving rod can be accelerated, and the fusion property among the raw materials can be improved.
According to the fig. 9-10: the outer surface wall of the driving rod 233 is fixedly connected with a gear A235, the outer surface wall of the gear A235 is in meshed connection with a plurality of gears B236, the tops of the gears B236 are fixedly connected with a linkage rod 237, the outer surface walls of the linkage rods 237 are movably inserted into the movable groove B232, the inner surface wall of the driving groove 230 is movably embedded with a driving block 238, the bottom of the driving block 238 is fixedly connected with a movable plate 239, the inner surface wall of the movable plate 239 is in meshed connection with the outer surface wall of the gears B236, the bottom of the movable plate 239 is fixedly connected with three scraping plates 240, the outer surface walls of the three scraping plates 240 are in contact with the inner surface wall of the culture dish 222, the gear A235 is fixedly sleeved between the outer surface walls of the driving rod 233, when the driving motor 225 drives the driving rod 233 to rotate, the gear A235 can be kept in linkage rotation, and is kept in meshed rotation with the gears B236, the tops of the gears B236 are respectively fixed with the rods 237, the inner surface of the movable groove B232 is rotated, and the three scraping plates are mutually meshed with the inner surfaces of the driving rod B236, and the inner surfaces of the driving rod B236 can be mutually meshed with the inner surfaces of the driving rod B236, and the inner surfaces of the driving rod B can be mutually meshed with the inner surfaces of the driving rod B236, and the driving rod B can rotate, and the driving rod can rotate by the driving rod 240 can be conveniently and rotated, and the driving rod can rotate by the driving rod 240.
According to the illustration in fig. 3: the support control mechanism 1 comprises a fixing frame 101, four bottom wheels 102 are arranged at the bottom of the fixing frame 101, a control box 103 is fixedly connected to the top of the fixing frame 101, a control panel 104 is arranged at the top of the control box 103, the four bottom wheels 102 are arranged at the bottom of the device, when the bottom wheels 102 rotate and are in contact with the ground, effective position moving treatment can be carried out on equipment, workers can convey the designation through the control panel 104, and finally the designation is transmitted to the position of each electric equipment by the control box 103 to carry out corresponding control treatment.
According to the illustration in fig. 3: the output end of the control box 103 is fixedly communicated with a wire 106, the output end of the wire 106 is fixedly communicated with a plurality of electromagnetic valves 107, the output ends of the electromagnetic valves 107 are fixedly communicated with a conveying pipe 108, the bottoms of the electromagnetic valves 107 are fixedly connected with the bottom of the fixing frame 101, firstly, an external conveying raw material pipeline is respectively communicated with the electromagnetic valves 107, the control box 103 is used for effectively controlling the communication of the conveying channel, and proper oxygen, pH values and the like can be provided for the inside of the equipment through the electromagnetic valves 107, so that the biological enzyme in the equipment can be effectively kept to fully react.
According to the figures 3-10: the output ends of the conveying pipes 108 are all communicated with the inside of the culture dish 222, one side of the outer wall of the mounting plate 213 is fixedly connected with one side of the outer wall of the fixing frame 101, the input end of the compressor 218 is communicated with the output end of the control box 103 through an internal line group, one side of the outer wall of the two mounting frames 228 is fixedly connected with one side of the outer wall of the fixing frame 101, one side of the outer wall of the mounting plate 213 is fixedly connected with one side of the outer wall of the fixing frame 101 at first, the fixed connection state between the support control mechanism 1 and the culture mechanism 2 can be completed, and the stability of the support control mechanism during operation is kept.
The working principle of the whole mechanism is as follows: firstly, when the biological enzyme reaction is performed, different raw materials can be added into the culture dish 222 through the feeding pipe 226, at the moment, the specification is conveyed at the position of the control panel 104, and finally, the specification is conveyed into the driving motor 225 by the control box 103, when the driving motor 225 is electrified, the output end of the driving motor 225 keeps rotating and drives the driving rod 233 to carry out linkage treatment, the driving rod 233 keeps a fixed state with the gear A235 so as to keep the linkage state of the driving rod and the gear A235, the gear A235 is meshed with the plurality of gears B236, the top of the plurality of gears B236 is respectively fixed with a linkage rod 237, the linkage rod 237 can move in the movable groove B232, thereby providing a driving point for the rotation of the plurality of gears B236, when the plurality of gears B236 are respectively meshed with the movable disc 239, the driving motor 225 drives the driving rod 233 and the gear A235 to rotate, and under mutual meshing transmission, the movable plate 239 drives the three scraping plates 240 and the driving rod 233 to generate opposite rotation treatment with the same frequency, the plurality of groups of stirring blades 234 can stir raw materials in the culture dish 222 to perform rapid fusion reaction, the three scraping plates 240 can be rotationally contacted with the inner surface wall of the culture dish 222 under the driving of the movable plate 239 so as to scrape the raw materials remained on the inner surface wall of the culture dish 222 and avoid adhesion of the raw materials on the inner wall of the culture dish 222, and in the reaction process, the control box 103 can be used for completing starting the plurality of electromagnetic valves 107, so that oxygen and pH value can be added into the culture dish 222, thereby effectively keeping the biological enzymes in the culture dish 222 fully react, and simultaneously, the heating treatment needs to be performed inside the culture dish, the electric power is transmitted to the position of the heating pipe 206, the inside of the heating pipe 206 generates resistance and starts heating, the heat can be transferred through the inner pipe 205, the liquid metal in the inner tank 207 can be heated and transferred into the shell conduit 203, and the shell conduit 203 is used as a medium, the bottom of the culture dish 222 is continuously heated, so that the heat is transferred into the equipment, the temperature requirement is provided for the reaction, after the later reaction is finished, the equipment is required to be cooled, two switch valves A208 are opened, the high-temperature liquid metal in the inner tank 207 can be transferred, the normal-temperature liquid metal is transferred into the inner tank 207, the heat transfer is isolated, the cooling treatment is implemented, the cooling efficiency is improved, when the subsequent low-temperature treatment and rapid cooling are required, the control box 103 is used for conveying the specification and starting the compressor 218, the compressor 218 sucks the low-temperature low-pressure gaseous condensing agent, and the compressor 218 can absorb the low-temperature low-pressure gaseous condensing agent under the compression of the self, the gas with high temperature and high pressure formed by the condensing agent is kept to enter the condensing tube 219 for cooling and radiating, and part of the gas entering the condensing tube 219 becomes medium-temperature liquid after condensation, at this time, the medium-temperature liquid enters the capillary tube 220, the pressure suddenly decreases due to the sudden narrowing at the position of the capillary tube 220, the part of the liquid suddenly evaporates and cools due to the sudden decrease of the pressure, the cooled liquid is evaporated and gasified in the evaporator 221, so that the cooling and radiating treatment of the outer surface wall of the evaporator 221 can be implemented, the low temperature generated by the outer surface wall of the evaporator 221 can be drawn to the heat conducting plate 217, the cooling treatment of the top liquid metal is completed, and under the micro pump 211 as the conveying power, and the conduit A209 and the conduit B212 are taken as conveying paths, the cooled liquid metal can be supplied to the interior of the inner tank 207, so that the temperature can be transferred to the interior of the culture dish 222 by the above-described means while maintaining the low temperature, and the low temperature treatment can be performed on the biological enzymes in the interior.
Although the present invention has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present invention.

Claims (10)

1. An enzyme-transformed biological culture device, characterized in that: the device comprises a support control mechanism (1), wherein the top of the support control mechanism (1) is fixedly connected with a culture mechanism (2);
the culture mechanism (2) comprises a base (201), a fixed slot (202) is formed in the bottom of the inner wall of the base (201), an outer shell conduit (203) is fixedly inserted between the inner surface walls of the fixed slot (202), the outer surface walls of the outer shell conduit (203) movably penetrate through the inside of the base (201), a plurality of groups of connecting rods (204) are fixedly connected to the inner surface walls of the outer shell conduit (203), an inner tube (205) is fixedly connected between the inner surface walls of the connecting rods (204), a heating tube (206) is fixedly connected to the inner surface walls of the inner tube (205), an inner groove (207) is formed between the outer shell conduit (203) and one side of the outer wall of the inner tube (205), two switch valves A (208) are fixedly connected to the outer wall of the inner tube (205), one of the two switch valves A (208) is fixedly connected with a conduit A (209), the output end of the conduit A (209) is fixedly connected to the output end of the micro pump (210), the micro pump (211) is fixedly connected to the output end (211) of the micro pump (211), and the output end of the conduit B (212) is fixedly communicated with the other input end of the two switch valves A (208).
2. The enzyme-converted biological culture device of claim 1, wherein: the bottom fixedly connected with mounting panel (213) of bin (210), the inner wall bottom of base (201) is fixed to be inserted and is equipped with and carry annular pipe (214), the bottom of carrying annular pipe (214) is fixed to be linked together and is had ooff valve B (215), the output of ooff valve B (215) is fixed to be linked together and is had output tube (241), the top of carrying annular pipe (214) is fixed to be linked together and is had multiunit leak hole (216).
3. An enzymatically converted biological culture apparatus as claimed in claim 2, wherein: the inner surface wall fixedly connected with heat conduction board (217) of bin (210), the bottom fixedly connected with compressor (218) of bin (210), the output fixedly connected with condenser pipe (219) of compressor (218), and the exterior wall of condenser pipe (219) contacts with the inner wall bottom of bin (210), the output fixedly connected with capillary (220) of condenser pipe (219), the output fixedly connected with of capillary (220) has evaporimeter (221), and the exterior wall of evaporimeter (221) contacts with the bottom of heat conduction board (217).
4. An enzymatically converted biological culture device of claim 3, wherein: the utility model discloses a culture dish, including base (201), interior table wall activity of base (201) is inserted and is equipped with culture dish (222), the exterior wall of culture dish (222) is provided with observation port (223), sealed lid (224) are passed through in the hinge upset at the top of culture dish (222), the top fixedly connected with driving motor (225) of sealed lid (224), and the rotation end activity of driving motor (225) runs through in the inside of sealed lid (224), the top fixed intercommunication of sealed lid (224) has inlet pipe (226), interior table wall activity of inlet pipe (226) is inlayed and is equipped with sealing plug (227).
5. The enzyme-converted biological culture device of claim 4, wherein: the outer surface wall fixedly connected with two mounting brackets (228) of culture dish (222), the interior surface wall fixedly connected with fixed disk (229) of culture dish (222), drive changeing groove (230) have been seted up to the bottom of fixed disk (229), movable groove A (231) have been seted up in the top center department of fixed disk (229), a plurality of movable grooves B (232) have been seted up at the top of fixed disk (229).
6. The enzyme-converted biological culture device of claim 5, wherein: the inner surface wall of the movable groove A (231) is movably inserted with a driving rod (233), the rotating end of the driving motor (225) is fixedly connected with the top of the driving rod (233), and a plurality of groups of stirring blades (234) are fixedly sleeved on the outer surface wall of the driving rod (233).
7. The enzyme-converted biological culture device of claim 6, wherein: the utility model discloses a dish, including drive bull stick (233), gear A (235)'s outward appearance wall meshing is connected with a plurality of gears B (236), a plurality of the top of gear B (236) is all fixedly connected with gangbar (237), and the outward appearance wall of a plurality of gangbars (237) is all movably inserted and is established in the inside of movable groove B (232), the interior surface wall activity of driving bull stick (230) is inlayed and is equipped with and drives rotating block (238), the bottom fixedly connected with movable dish (239) of driving rotating block (238), and the interior surface wall of movable dish (239) is connected with the outward appearance wall meshing of a plurality of gears B (236), the bottom fixedly connected with three scraper blade (240) of movable dish (239), and the outward appearance wall of three scraper blade (240) all contacts with the interior surface wall of culture dish (222).
8. The enzyme-converted biological culture device of claim 7, wherein: the support control mechanism (1) comprises a fixing frame (101), four bottom wheels (102) are arranged at the bottom of the fixing frame (101), a control box (103) is fixedly connected to the top of the fixing frame (101), and a control panel (104) is arranged at the top of the control box (103).
9. The enzyme-converted biological culture device of claim 8, wherein: the output of control box (103) fixedly communicates there is wire (106), the output of wire (106) fixedly communicates there is a plurality of solenoid valves (107), a plurality of the output of solenoid valves (107) all fixedly communicates there is conveyer pipe (108), and the bottom of a plurality of solenoid valves (107) all is connected with the bottom fixed of mount (101).
10. The enzyme-converted biological culture device of claim 9, wherein: the output ends of the conveying pipes (108) are communicated with the inside of the culture dish (222), one side of the outer wall of the mounting plate (213) is fixedly connected with one side of the outer wall of the fixing frame (101), the input end of the compressor (218) is communicated with the output end of the control box (103) through an internal line group, and one side of the outer wall of the mounting frame (228) is fixedly connected with one side of the outer wall of the fixing frame (101).
CN202311401013.5A 2023-10-26 2023-10-26 Biological culture device for enzyme conversion Pending CN117126736A (en)

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Application publication date: 20231128