CN117122054A - Instant bird's nest product formula suitable for pregnant women - Google Patents

Instant bird's nest product formula suitable for pregnant women Download PDF

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Publication number
CN117122054A
CN117122054A CN202311136761.5A CN202311136761A CN117122054A CN 117122054 A CN117122054 A CN 117122054A CN 202311136761 A CN202311136761 A CN 202311136761A CN 117122054 A CN117122054 A CN 117122054A
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parts
vitamin
bird
nest
mice
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蒋林
张小玲
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Guangxi Jinpin Yanzhuang Food Co ltd
Sun Yat Sen University
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Guangxi Jinpin Yanzhuang Food Co ltd
Sun Yat Sen University
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Priority to CN202311136761.5A priority Critical patent/CN117122054A/en
Publication of CN117122054A publication Critical patent/CN117122054A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A23L33/155—Vitamins A or D
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention relates to the technical field of food, and in particular discloses an instant bird's nest product suitable for pregnant women, which comprises the following components: 1-25 parts of bird's nest and 1-30 parts of bird's nest mate; the bird's nest mate comprises the following components: at least one of sugar, probiotics, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin B6, folic acid, vitamin B12, vitamin C, vitamin D and vitamin E. The formula bird's nest in the invention has the functions of improving immunity, resisting oxidation, regulating normal secretion of hormone and the like, and can supplement nutrition for pregnant women and improve fetal quality.

Description

Instant bird's nest product formula suitable for pregnant women
Technical Field
The invention belongs to the technical field of health-care food processing, and relates to a formula of an instant bird's nest product suitable for pregnant women.
Background
The bird's nest has flat nature, sweet taste, lung tonifying and yin nourishing, and the main nutritional ingredients of the bird's nest include protein, amino acid and sialic acid. Sialic acid is ubiquitous in mammals and has a high content in breast milk. In recent years, the edible bird's nest is increasingly consumed in China, and most of consumers are pregnant women, puerperal women or women with pregnancy. Many reports prove that the bird's nest is beneficial to pregnant women, and the effect of tonifying both mother and infant can be achieved by eating the bird's nest. The bird's nest can improve immunity of human body, and keep pregnant woman healthy during pregnancy and lactation, and can resist striae gravidarum and inhibit puerperal pigmentation. After childbirth, the bird's nest is eaten, which is helpful for the recovery of the body. The sialic acid glycoprotein contained in the bird's nest can directly stimulate cell division, regeneration and tissue reconstruction, helps the recovery of the uterus and the birth canal of a puerpera, and plays a great role in nourishing and recovering the pregnant and puerpera. In addition, the edible bird's nest is helpful for milk secretion, and is helpful for improving the immunity and even intelligence of the offspring. Therefore, how to provide a healthy and nutritional instant bird's nest product formula suitable for pregnant women is a technical problem to be solved by the technicians in the field.
Disclosure of Invention
The formula of the instant bird's nest product is suitable for pregnant women, and is beneficial to the growth and development of fetuses and the physical health of the pregnant women.
The invention is realized by the following technical scheme: an instant bird's nest product suitable for consumption by pregnant women, comprising: 1-25 parts of bird's nest and 1-30 parts of bird's nest partner. The bird's nest mate comprises the following components: at least one of sugar, probiotics, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin B6, folic acid (vitamin B9), vitamin B12, vitamin C, vitamin D and vitamin E.
Further, the bird's nest mate comprises the following components in parts by weight: 30 parts of sugar, 12 parts of probiotics, 4 parts of vitamin A,4 parts of vitamin B1,4 parts of vitamin B2,4 parts of vitamin B3,4 parts of vitamin B6,2 parts of folic acid, 4 parts of vitamin B12,4 parts of vitamin C,4 parts of vitamin D and 8 parts of vitamin E.
Further, the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,2 parts of vitamin B3,2 parts of vitamin B6,1 part of folic acid, 2 parts of vitamin B12,2 parts of vitamin C,2 parts of vitamin D and 4 parts of vitamin E.
Further, the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,2 parts of vitamin B3,2 parts of vitamin B6,1 part of folic acid, 2 parts of vitamin B12,2 parts of vitamin C and 4 parts of vitamin E.
Further, the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,2 parts of vitamin B3,2 parts of vitamin B6,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
Further, the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,2 parts of vitamin B3,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
Further, the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
Further, the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
Further, the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
Further, the bird's nest is a dry bird's nest after cleaning and picking hair.
Further, the sugar is isomaltooligosaccharide.
Further, the probiotics are lactobacillus paracasei.
A brewing product comprises the instant edible bird's nest product suitable for pregnant women, and the instant edible bird's nest product suitable for pregnant women is prepared into a brewing product for brewing.
A beverage comprises the instant edible bird's nest product suitable for pregnant women, and the instant edible bird's nest product suitable for pregnant women is prepared into a direct-drinking product or a heating drinking product.
The synergistic functional bird's nest product comprises the instant bird's nest product suitable for pregnant women, and the instant bird's nest product suitable for pregnant women is prepared into the synergistic functional bird's nest product according to a set proportion.
The invention also provides a preparation method of the instant bird nest suitable for pregnant women, which comprises the following steps: s1, weighing bird ' S nest and bird ' S nest mate according to a proportion, wherein the bird ' S nest is foamed by ten times the volume of purified water for standby; s2, stewing the bird 'S nest for 30min, cooling, adding the bird' S nest mate, then putting the bird 'S nest mate into a container, and sterilizing to obtain the instant bird' S nest suitable for pregnant women.
Compared with the prior art, the invention has the advantages that: the formula bird's nest in the invention has the functions of improving immunity, resisting oxidation, regulating normal secretion of hormone and the like, and can supplement nutrition for pregnant women and improve fetal quality.
Detailed Description
The following describes in detail the examples of the present invention, which are implemented on the premise of the technical solution of the present invention, and detailed embodiments and specific operation procedures are given, but the scope of protection of the present invention is not limited to the following examples.
The example discloses an instant bird's nest suitable for pregnant women to eat, which comprises the following components in parts by weight: 25 parts of bird's nest and 30 parts of bird's nest mate. The bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
The main therapeutic efficacy of the ingredients in the bird's nest mate of the invention is as follows:
the vitamin A has effects in promoting bone growth and development, promoting cell proliferation, maintaining vision, maintaining skin health, promoting synthesis of immunoglobulin, inhibiting tumor growth, resisting aging, and preventing alopecia. Vitamin a deficiency can lead to night blindness and anemia symptoms; children patients then affect growth.
Vitamin B1 is involved in the oxidative metabolism of sugars in the body on the one hand; on the other hand, the anticholinesterase effect is exerted. When it is absent, it results in reduced acetylcholine in the body, affecting nerve conduction.
Vitamin B2 can protect eyesight, repair cells and promote growth and development.
Vitamin B3 has the function of maintaining the digestive system function of the body and is involved in the synthesis of sex hormones.
Vitamin B6 has the functions of promoting human development, inhibiting vomiting and the like, and participates in the metabolism of amino acids in the body. The deficiency of vitamin B6 can cause vomiting, cramp and the like.
Vitamin B12 has effects of resisting fatty liver, promoting vitamin A enrichment and cell development metabolism.
Vitamin C is a nutritional supplement and an antioxidant. Can remove free radicals in vivo, has anticancer, arteriosclerosis resisting, rheumatism treating, and cataract preventing effects by supplementing vitamin C.
Vitamin D has effects of resisting rickets, promoting skin cell growth, differentiating and regulating immunity. Plays an important role in maintaining the intestinal integrity and the balance of the intestinal environment between the host and the intestinal flora.
Vitamin E is an antioxidant with anticoagulant, vasodilating, immunity enhancing and antiaging effects.
Folic acid participates in synthesis of heme, promotes fetal development, protects cardiac vessels, and reduces the occurrence of AD. Can be used for treating folic acid deficiency caused by various reasons and megaloblastic anemia caused by folic acid deficiency. Prophylactic treatment to reduce the risk of fetal neural tube defects.
The invention also provides a pharmacological verification method of the instant bird nest suitable for pregnant women, which comprises the instant bird nest suitable for pregnant women, and the specific steps are as follows.
1.1 laboratory animals and feeding conditions
a) Experimental animal
Grade, germ line: SPF grade ICR mice.
Animal management: animals are kept and managed by personnel qualified for experimental animal management.
Age, number, sex at purchase: 8 weeks of age, 80 females and 40 males.
Breeding unit: hunan Stokes Jingda laboratory animal Co., ltd., the laboratory animal production license number is: SCXK (xiang) 2019-0004, animal quality eligibility: 430727220102027764.
quarantine process: animals were quarantined and observed for 5 days. During this period, the animals were observed for signs of appearance, behavioral activity, fecal properties, body weight, diet, and the like.
b) Feeding conditions
Feeding room: center for tumor control in Zhongshan university (center for experimental animals). Experimental animal use license number: SYXK (Yue) 2020-0102.
Temperature and humidity: 20-25 ℃; humidity: 40% -70%.
Ventilation times: greater than 10 times per hour.
Feeding density: 5-10/cage.
Illumination time: 12 hours (7:00 am on-7:00 pm off).
c) Feed stuff
Category: SPF-grade rat feed
The feeding method comprises the following steps: free ingestion
The conventional nutrient composition index of the feed is as follows: through detection by Guangdong province laboratory animal monitoring institute (refer to national standard of the people's republic of China GB 14924.3-2010), detection frequency is that: twice a year.
And (3) preserving feed: is stored in a special fodder room, and kept ventilated, clean and dry
d) Drinking water
Type of drinking water: 121 ℃ (1.0 kg/cm) 2 ) Sterilizing high-quality water for 30min, and meeting the standard of drinking Water quality (CJ 94-2)005)。
The water supply method comprises the following steps: can be taken by animals.
e) Animal carcass treatment
The animal carcasses are temporarily stored in a refrigerator special for minus 20 ℃ in an animal temporary storage room and are concentrated to a Guangdong living environment harmless treatment center for harmless treatment.
1.2 major instruments and reagents
a) Main instrument
MG416A electronic balance, yiwu Moger electronic technologies Co., ltd;
TLE 104 electronic balance, meltler-tolido instruments (Shanghai);
MIKRO 200R high-speed refrigerated centrifuge, hettich, germany;
an Epoch2 microplate spectrophotometer, biotek company, usa;
eclipse Ti-S microscope, nikon Corp.
b) Main reagent
Superoxide dismutase (SOD), a company of biotechnology limited of shanghai bi yun;
malondialdehyde (MDA) detection kit, shanghai Biyun biotechnology Co., ltd;
IgG, igA, IL-6, TNF-a assay kit, abcam;
ChAT and AchE activity detection kit, nanjing builds the institute of biological engineering.
1.3 Experimental methods
a) Sample dosage and feeding route
The common bird's nest is converted into the feeding dosage of the mice according to the human consumption, the common bird's nest is 1.0g/kg, and the dosage in the formula bird's nest is as follows: bird's nest 1.0 g/kg+bird's nest partner 1.2g/kg, formula bird's nest high dose: 2g/kg of bird's nest and 2.4g/kg of bird's nest partner, and the stomach irrigation volume is 20mL/kg.
b) Preparation of test samples
B. Preparing a sample of a common bird's nest group: 1.0g/kg of bird's nest, weighing 10g of bird's nest, adding distilled water to 200mL, uniformly mixing, soaking for 4h, preparing into 200mL of bird's nest dosage in water bath at 100 ℃, subpackaging at-20 ℃ for preservation, and melting with hot water bath before use.
C. Dosage group test sample preparation in formula bird's nest: 1.0g/kg of bird's nest and 1.2g/kg of partner, weighing 10g of bird's nest and 12g of partner, adding distilled water to 200mL of the mixture, uniformly soaking for 4h, preparing into 200mL of bird's nest dosage in a water bath at 100 ℃ for 30min, subpackaging for preservation at-20 ℃, and melting by using a hot water bath before use.
D. Preparing a high-dose group test sample of formula bird's nest: 2.0g/kg of bird's nest and 2.4g/kg of partner, weighing 20g of bird's nest and 24g of partner, adding distilled water to 200mL of the mixture, uniformly soaking for 4h, preparing 200mL of bird's nest into high dose in a water bath at 100 ℃, subpackaging the high dose, preserving the high dose at-20 ℃, and melting the high dose in a hot water bath before use.
c) Grouping and handling of animals
SPF grade 8-10 week old ICR 96 were taken, of which female 64 and male 32 were selected. Female mice were randomly divided into 4 groups according to body weight, respectively: A. blank control group, B. Common bird's nest group, C. Formula bird's nest medium dose group, D. formula bird's nest high dose group, 16 in each group, after feeding for 7 days in advance, female mice were intraperitoneally injected with PMSG 5 IU/one, after 48 hours, HCG 5 IU/one was injected 2-3 hours before endogenous LH hormone release, then female mice and male mice were caged in a 2:1 ratio, and on the next day, examination of female mice vaginal suppositories and vaginal secretions was performed around 9 a morning. Mice with pessaries or sperm secretion were scored as pregnant on day 0.5 and each test sample and positive control were given daily lavage to day 15 with a lavage volume of 20mL/kg and the blank group given daily equal volumes of distilled water.
d) Detecting index for pregnant mice
The mother mouse behaviours were observed on day 18, part of pregnant mice were sacrificed and dissected at the dislocation on day 19 of pregnancy, and the index of the test sample on the pregnant mice was detected.
Female mouse body weight and general status observation
The state of animals (appearance signs, behavioral activities, fecal properties, feeding conditions, etc.) was observed daily; mice were weighed at pregnancy 0, 3, 7, 14, 19 d.
Day 18 behavioural observations of the master mice.
Open field experiment
The test mice were individually placed in the center of a novel open square box (50×50×30 cm) in sequence, and the activity of the animals in the open field, including the number of central area entries, the time the central area stayed, and the number of erections, was recorded for 5min.
Overhead plus maze experiment:
the plus maze comprises a central platform (5 x 5 cm), two open arms and two closed arms (30 x 5 cm), which are set up 55cm above the floor. The closing arm has a wall 10cm high, the end being closed and the opening arm being fully open. The mice were placed on a central platform with their heads facing the open arms and allowed to freely enter the four arms for 5min. The number of times the mice entered the open and closed arms and the time they remained on these arms were measured.
Tail suspension experiment
The tail suspension experiment adopts self-made equipment to fix the tail of an experimental mouse, so that the head of the experimental mouse hangs downwards, the mouse struggles in the environment to try to get rid of the dilemma, and after the experimental mouse is striven and still cannot get rid of, the experimental mouse is in intermittent state and is judged to be in relative rest time. The relative rest time includes, but is not limited to, a completely rest state, and the minute shake of the mouse limb is also determined as a relatively rest state, and the relative rest time is counted.
Female mouse immunity index
IgG, igA, IL-6, TNF-alpha in serum
The mice were collected from the eyeballs and blood was collected, placed at room temperature for about 1 hour, centrifuged at 3000rpm for 15min to separate serum, stored at-80℃and assayed for serum cytokine (IgG, igA, IL-6, TNF-a) content according to the assay kit instructions.
Index of spleen and thymus
Mice were sacrificed after blood collection, spleen and thymus were dissected after intact uterus was dissected, the viscera surface blood was blotted with filter paper, weighed, and spleen (thymus) index calculated according to the formula, spleen (thymus) index = spleen (thymus) mass (mg)/body weight (g).
Female fertility and fetal mouse mass:
after blood collection, the whole uterus is dissected and further dissected, the total weight of the fossa and the total weight of the placenta are weighed by an analytical balance, the number of mice is recorded (the number of live fetuses, dead fetuses and absorbed fetuses are recorded), and the physique of the mice (the weight, the body length, the tail length and the appearance of the live mice) is recorded.
g) Action detection index on mice
After the rest pregnant mice are naturally produced, offspring observation experiments are carried out, each group of female mice is subjected to gastric lavage for 21 days, the gastric lavage volume is 20mL/kg, the blank control group is subjected to daily administration of equal volume of distilled water, and 15 mice in each group are indirectly interfered with the mice through feeding milk through the female mice.
Body constitution observation of mice
Observing physical indexes of the mice in 1, 7, 14 and 21 days: body weight, length, tail length, shape, etc.
Behavioral observations of mice
The mice were observed for day 21 behavioural behavior in the same manner as under item "4.6.2".
Mice immunity index:
blood is taken, the separated serum is preserved at the temperature of minus 80 ℃, the content of serum cytokines (IgG, igA, IL-6) is detected, then spleen and thymus are dissected, the surface blood stain of the viscera is sucked by filter paper, the spleen (thymus) index is calculated according to a formula, and the spleen (thymus) index=spleen (thymus) quality (mg)/body weight (g).
1.1. Brain tissue index of mice
Brain index
The brains of the mice were weighed with an analytical balance and the brain index = brain mass (mg)/body weight (g) was calculated according to the formula.
Antioxidant index in brain tissue
The brain tissue of the mice is weighed, and then the left hemisphere of the mice is uniformly taken and stored at the temperature of minus 80 ℃ for detecting the oxidation resistance index, and the superoxide dismutase (SOD) activity and the Malondialdehyde (MDA) content are detected according to the instruction of the kit.
Activity detection of ChAT and AchE in cerebral cortex
The rat brain tissue is weighed, then the isolated brain right hemisphere cortex tissue is preserved at-80 ℃ and used for detecting the activities of the Chut and the AchE in the cortex, and the activities of the Chut and the AchE in the brain cortex are detected according to the instruction book of the kit.
1.4 data processing
Experimental data were statistically processed by GraphPad Prism 9.2 biometric software: the metering data are expressed in mean±sd and are analyzed using analysis of variance in combination with Dunnett's multiple comparison.
1.5 experimental results
a) Influence of the sample on the weight and general status of pregnant mice
The animal groups have good mental state, smooth fur color, free movement, uniform breathing, no obvious abnormality of food intake and feces, and no abnormal secretion in the mouth and nose during the experimental period. From Table 1, it can be seen that the animals of each group had a normal weight gain during each period of feeding, and that the pregnant mice of each sample group had a significant weight gain (P < 0.01) compared to the blank group.
TABLE 1 influence of the test samples on the weight of pregnant mice (mean.+ -. SD, n=9)
Compared with blank group, p <0.01, p <0.05
b) Behavioral effects of test samples on pregnant mice
As can be seen from table 2, the number of erection times of pregnant mice in the formula bird's nest high dose group is increased (p < 0.05) to a certain extent compared with that in the blank control group.
The results of the 18d elevated maze of pregnant female mice are shown in Table 3, which shows an increase in the number of times and residence time of the high dose group of formula bird's nest into the open arm (p < 0.05) compared to the blank control group.
The results of the mother pregnancy 18d tail suspension experiments show that the formula bird's nest high dose group has a certain increase (p < 0.05) relative to the rest time compared with the blank control group as shown in table 4.
TABLE 2 influence of the test sample on the field test of pregnant mice (mean+ -SD, n=9)
Compared with blank group, p <0.01, p <0.05
Table 3. Influence of test sample on elevated maze of pregnant mice (mean±sd, n=9)
Compared with blank group, p <0.01, p <0.05
Table 4. Influence of the test sample on the tail suspension experiment of pregnant mice (mean.+ -. SD, n=9)
Compared with blank group, p <0.01, p <0.05
c) Influence of the test sample on fertility of the female mouse
The total weight of the littermates and the total weight of the placenta of the pregnant female mice were 19d, and as can be seen from table 5, the total weight of the placenta was increased (p < 0.05) in the formula bird's nest high dose group compared to the blank control group.
As seen from table 6, the number of live fetuses of each sample group is not significantly different (p > 0.05) from that of the blank control group, and the number of dead fetuses and the number of absorbed fetuses of the dose group in the formula bird's nest and the high-dose group in the formula bird's nest are reduced to a certain extent (p < 0.05).
As can be seen from table 7, compared with the blank control group, the average body weight of the bird's nest high-dose group child is significantly increased (P < 0.05), the average body length of the bird's nest high-dose group child is significantly increased (P < 0.01), and no significant difference (P > 0.05) is seen in the rat tail length of each sample group.
Table 5. Influence of test sample on total weight of fossa and placenta (mean±sd, n=9)
Compared with blank group, p <0.01, p <0.05
TABLE 6 influence of the test samples on the number of parity (mean.+ -. SD, n=9)
Compared with blank group, p <0.01, p <0.05
TABLE 7 influence of the test samples on the constitution of the miscarriage (mean.+ -. SD, n=9)
Compared with blank group, p <0.01, p <0.05
d) Influence of the test sample on the immunocompetence of the female mouse
As shown in Table 8 and Table 9, the serum of pregnant female mice has a tendency of increasing the IgG content (P < 0.05), the serum of female mice has a tendency of increasing the IL-6 and TNF-alpha content, and the serum of female mice has a tendency of increasing the IL-6 and TNF-alpha content (P < 0.05).
As can be seen from table 10, the spleen index of the mice in the d. Formula bird's nest high dose group tended to increase (P < 0.05) compared to the blank group, and the thymus index of the mice in each sample group did not significantly differ (P > 0.05).
Table 8. Influence of the test sample on IgG, igA in serum (mean.+ -. SD, n=9)
Compared with blank group, p <0.01, p <0.05
TABLE 9 influence of the test samples on IL-6, TNF- α in serum (mean.+ -. SD, n=9)
Compared with blank group, p <0.01, p <0.05
Table 10. Influence of the test sample on spleen and thymus index of pregnant mice (mean.+ -. SD, n=9)
Compared with blank group, p <0.01, p <0.05
e) Influence of the test sample on the constitution of mice
As shown in Table 11, the weight of the mice in the dosage group 1d and 7d in the formula bird's nest tends to increase (P < 0.05) compared with the blank control group, and the weight of the mice in the high dosage group 1d, 7d, 14d and 21d in the formula bird's nest tends to increase (P < 0.05).
As a result of the body length of the mice in 1d to 21d, it can be seen from Table 12 that the body lengths of the mice in the dosage group 7d and 21d in the formula bird's nest are increased (P < 0.05) and the body lengths of the mice in the high dosage group 7d, 14d and 21d in the formula bird's nest are increased (P < 0.05) compared with the blank control group.
From Table 13, it can be seen that the tail lengths of the mice 7d and 21d in the high-dose group of formula bird's nest have an increasing tendency (P < 0.05) compared with the blank group.
Table 11. Influence of the test sample on the weight of mice (mean.+ -. SD, n=15, unit g)
Compared with blank group, p <0.01, p <0.05
Table 12. Influence of the sample on the stature of the mice (mean.+ -. SD, n=15, unit cm)
Compared with blank group, p <0.01, p <0.05
Table 13. Influence of the test sample on the length of the tail of the mice (mean.+ -. SD, n=15, unit cm)
Compared with blank group, p <0.01, p <0.05
f) Effects of test samples on mouse behaviours
As shown in table 14, compared with the blank control group, d. The formula bird's nest high dose group mice have a trend of increasing residence time in the central area of open field, the number of times of entering the central area of open field and the number of erection times (P < 0.05).
The experimental results of 21d overhead maze of the born mice show that compared with a blank control group, the frequency, the residence time and the duty ratio of the open arms of the mice in the formula bird's nest high-dose group are all increased, and the frequency and the residence time duty ratio of the open arms of the mice in the formula bird's nest in the formula group are increased (P is less than 0.05).
The results of 21d tail suspension experiments performed on mice born are shown in Table 16, and compared with the blank control group, the B.common bird's nest group and the D.formula bird's nest high dose group have a reduced tendency (P < 0.05) relative to the rest time.
Table 14. Influence of the sample on the open field experiment of mice (mean.+ -. SD, n=15)
Compared with blank group, p <0.01, p <0.05
Table 15. Influence of the sample on the elevated maze of mice (mean.+ -. SD, n=15)
Compared with blank group, p <0.01, p <0.05
Table 16. Influence of the sample on the tail suspension experiment of mice (mean.+ -. SD, n=15)
Compared with blank group, p <0.01, p <0.05
g) Influence of the test sample on the immunity of the mice
As shown in Table 17, the serum of the mice born with 22d mice has a tendency of increasing the content of IgG, igA and IL-6 (p < 0.05) in the serum of the mice in the high-dose group of formula nidus Collocaliae, and the serum of the mice in the dose group of formula nidus Collocaliae has a tendency of increasing the content of IL-6 (p < 0.05) compared with the serum of the mice in the blank group.
The results of immune organs of the mice born at 22d are shown in Table 18, compared with a blank control group, the thymus of the mice in the common bird's nest group and the dosage group in the formula bird's nest group are increased, and the spleen index and thymus of the mice in the formula bird's nest high dosage group are both increased (p is less than 0.05).
Table 17. Influence of the test sample on IgG, igA, IL-6 in serum (mean.+ -. SD, n=10)
Compared with blank group, p <0.01, p <0.05
Table 18. Influence of the sample on spleen and thymus index of mice (mean.+ -. SD, n=15)
Compared with blank group, p <0.01, p <0.05
h) Influence of the sample on the brain tissue of the mice
The brain index results of the 22d mice born are shown in table 19. It can be seen that the brain index of the d-formula bird's nest high dose group mice is significantly increased (p < 0.05) compared to the blank control group.
As shown in Table 20, compared with the blank control group, the MDA content in brain tissue of the mice in the formula bird's nest dose group and the formula bird's nest high dose group has a tendency to decrease (p < 0.05), and the SOD activity of brain tissue of the mice in the formula bird's nest high dose group has a tendency (p < 0.05).
Acetylcholine transferase (ChAT) and acetylcholinesterase (AchE) are two important enzymes in brain for synthesis and hydrolysis of the neurotransmitter acetylcholine, whose activity directly affects the content of acetylcholine in the brain. From table 21, it can be seen that the activity of acetylcholine transferase (ChAT) and acetylcholinesterase (AchE) in the cerebral cortex of the newborn mice at birth 22d is increased (p < 0.05) in the c-formulation bird's nest dose group and d-formulation bird's nest high-dose group in the cerebral cortex tissue of the newborn mice compared to the blank control group; B. the AChE activity in rat brain cortex tissues of common bird's nest group and D. formulated bird's nest high dose group has a reduced tendency (p < 0.05).
Table 19. Influence of the test sample on the brain index of mice (mean.+ -. SD, n=15)
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Compared with blank group, p <0.01, p <0.05
Table 20. Influence of the sample on MDA and SOD in brain tissue of mice (mean.+ -. SD, n=10)
Compared with blank group, p <0.01, p <0.05
Table 21 is affected by the effect of the sample on ChAT, achE activities in rat cerebral cortex (mean±sd, n=10)
Compared with blank group, p <0.01, p <0.05
Conclusion: under the experimental conditions, the formula bird nest can obviously increase the body length and the body weight of the pregnant mice in the pregnant mice with 19 days, improve the open field, the overhead cross maze and the tail suspension behavioural index of the pregnant mice to a certain extent, increase the serum immune index IgG, IL-6, TNF-alpha content and immune organ spleen index of the pregnant mice, and improve the immunity of the pregnant mice to a certain extent. Influence of formula bird's nest on offspring: the bird's nest has the tendency of increasing the body weight, the body length and the tail length of the mice, has a certain effect on improving the constitution of the mice, has a certain improvement on the open field, the overhead cross maze and the tail suspension behavioural index of the mice, has a certain tendency of increasing serum immune index IgG, IL-6 content and immune organ spleen and thymus index of the mice, has a certain improvement on the immunity of the mice, has a remarkable increase on the brain index of the mice, has a tendency of reducing MDA content and improving SOD activity, has a tendency of increasing ChAT enzyme activity and reducing AchE activity, and has a certain improvement effect on the development of the brain of the mice.

Claims (15)

1. An instant bird's nest product suitable for consumption by a pregnant woman, comprising: 1-25 parts of bird's nest and 1-30 parts of bird's nest mate; the bird's nest mate comprises the following components: at least one of sugar, probiotics, vitamin A, vitamin B1, vitamin B2, vitamin B3, vitamin B6, folic acid, vitamin B12, vitamin C, vitamin D and vitamin E.
2. The instant bird's nest product suitable for pregnant women according to claim 1, wherein the bird's nest mate comprises the following components in parts by weight: 30 parts of sugar, 12 parts of probiotics, 4 parts of vitamin A,4 parts of vitamin B1,4 parts of vitamin B2,4 parts of vitamin B3,4 parts of vitamin B6,2 parts of folic acid, 4 parts of vitamin B12,4 parts of vitamin C,4 parts of vitamin D and 8 parts of vitamin E.
3. The instant bird's nest product suitable for pregnant women according to claim 1, wherein the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,2 parts of vitamin B3,2 parts of vitamin B6,1 part of folic acid, 2 parts of vitamin B12,2 parts of vitamin C,2 parts of vitamin D and 4 parts of vitamin E.
4. The instant bird's nest product suitable for pregnant women according to claim 1, wherein the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,2 parts of vitamin B3,2 parts of vitamin B6,1 part of folic acid, 2 parts of vitamin B12,2 parts of vitamin C and 4 parts of vitamin E.
5. The instant bird's nest product suitable for pregnant women according to claim 1, wherein the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,2 parts of vitamin B3,2 parts of vitamin B6,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
6. The instant bird's nest product suitable for pregnant women according to claim 1, wherein the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,2 parts of vitamin B3,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
7. The instant bird's nest product suitable for pregnant women according to claim 1, wherein the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,2 parts of vitamin B2,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
8. The instant bird's nest product suitable for pregnant women according to claim 1, wherein the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,2 parts of vitamin B1,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
9. The instant bird's nest product suitable for pregnant women according to claim 1, wherein the bird's nest mate comprises the following components in parts by weight: 15 parts of sugar, 6 parts of probiotics, 2 parts of vitamin A,1 part of folic acid, 2 parts of vitamin C and 4 parts of vitamin E.
10. The instant bird's nest product for pregnant women according to claim 1, wherein the bird's nest is a dried bird's nest after washing and picking.
11. The ready-to-eat bird's nest product suitable for consumption by pregnant women according to claim 1, wherein the sugar is isomaltooligosaccharide.
12. The ready-to-eat bird's nest product suitable for pregnant women according to claim 1, wherein the probiotics are lactobacillus paracasei.
13. A brewing product characterized in that the instant bird's nest product suitable for pregnant women is prepared into a brewing product for brewing, wherein the instant bird's nest product suitable for pregnant women is contained in the instant bird's nest product suitable for pregnant women according to claim 1.
14. A beverage comprising the instant bird's nest product of claim 1 suitable for pregnant women, wherein the instant bird's nest product suitable for pregnant women is prepared for direct drinking or for heating drinking.
15. The synergistic functional bird's nest product is characterized by comprising the instant bird's nest product suitable for pregnant women, which is disclosed in claim 1, and the synergistic functional bird's nest product is prepared according to a set proportion.
CN202311136761.5A 2023-09-05 2023-09-05 Instant bird's nest product formula suitable for pregnant women Pending CN117122054A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106036891A (en) * 2016-06-30 2016-10-26 王丽芬 Plant pure dew/flower dew swallow nests and preparation method thereof
CN106579375A (en) * 2016-11-30 2017-04-26 洛阳华以生物工程有限公司 Edible bird's nest collagen peptide composite powder and preparation technology thereof
CN107348483A (en) * 2017-07-05 2017-11-17 阎艺方 A kind of pregnant and lying-in women's full function nutritious supplementary pharmaceutical
CN110663943A (en) * 2019-11-07 2020-01-10 厦门贰拾叁号生物科技有限公司 Instant freeze-dried cubilose product and method thereof
CN110839883A (en) * 2019-12-03 2020-02-28 厦门贰拾叁号生物科技有限公司 Freeze-dried instant edible bird's nest with hand-washing and preparation process thereof
CN113424953A (en) * 2021-07-06 2021-09-24 厦门市燕之屋丝浓食品有限公司 Instant cubilose product suitable for pregnant women and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106036891A (en) * 2016-06-30 2016-10-26 王丽芬 Plant pure dew/flower dew swallow nests and preparation method thereof
CN106579375A (en) * 2016-11-30 2017-04-26 洛阳华以生物工程有限公司 Edible bird's nest collagen peptide composite powder and preparation technology thereof
CN107348483A (en) * 2017-07-05 2017-11-17 阎艺方 A kind of pregnant and lying-in women's full function nutritious supplementary pharmaceutical
CN110663943A (en) * 2019-11-07 2020-01-10 厦门贰拾叁号生物科技有限公司 Instant freeze-dried cubilose product and method thereof
CN110839883A (en) * 2019-12-03 2020-02-28 厦门贰拾叁号生物科技有限公司 Freeze-dried instant edible bird's nest with hand-washing and preparation process thereof
CN113424953A (en) * 2021-07-06 2021-09-24 厦门市燕之屋丝浓食品有限公司 Instant cubilose product suitable for pregnant women and preparation method thereof

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