CN117070315A - Fermentation reactor for coenzyme Q10 and application thereof - Google Patents
Fermentation reactor for coenzyme Q10 and application thereof Download PDFInfo
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- CN117070315A CN117070315A CN202311322929.1A CN202311322929A CN117070315A CN 117070315 A CN117070315 A CN 117070315A CN 202311322929 A CN202311322929 A CN 202311322929A CN 117070315 A CN117070315 A CN 117070315A
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- tubular reactor
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- fermentation
- detection unit
- air inlet
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Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 115
- 230000004151 fermentation Effects 0.000 title claims abstract description 115
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 235000017471 coenzyme Q10 Nutrition 0.000 title claims abstract description 36
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 title claims abstract description 36
- 229940110767 coenzyme Q10 Drugs 0.000 title claims abstract description 36
- 238000010438 heat treatment Methods 0.000 claims abstract description 44
- 238000001514 detection method Methods 0.000 claims description 46
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 30
- 229910052760 oxygen Inorganic materials 0.000 claims description 30
- 239000001301 oxygen Substances 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 5
- 230000002093 peripheral effect Effects 0.000 claims description 5
- 239000002609 medium Substances 0.000 description 23
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 14
- 238000011218 seed culture Methods 0.000 description 13
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
- 239000001963 growth medium Substances 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 230000001276 controlling effect Effects 0.000 description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 description 8
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 8
- 239000002054 inoculum Substances 0.000 description 7
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 6
- 241000191043 Rhodobacter sphaeroides Species 0.000 description 6
- 229960002685 biotin Drugs 0.000 description 6
- 235000020958 biotin Nutrition 0.000 description 6
- 239000011616 biotin Substances 0.000 description 6
- 244000068988 Glycine max Species 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 5
- 108010080698 Peptones Proteins 0.000 description 5
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 5
- 238000007599 discharging Methods 0.000 description 5
- 239000011664 nicotinic acid Substances 0.000 description 5
- 229960003512 nicotinic acid Drugs 0.000 description 5
- 235000001968 nicotinic acid Nutrition 0.000 description 5
- 235000019319 peptone Nutrition 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 235000019157 thiamine Nutrition 0.000 description 5
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 5
- 229960003495 thiamine Drugs 0.000 description 5
- 239000011721 thiamine Substances 0.000 description 5
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 235000013379 molasses Nutrition 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 229960002477 riboflavin Drugs 0.000 description 4
- 235000019192 riboflavin Nutrition 0.000 description 4
- 239000002151 riboflavin Substances 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 239000012137 tryptone Substances 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 229910000365 copper sulfate Inorganic materials 0.000 description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000011790 ferrous sulphate Substances 0.000 description 3
- 235000003891 ferrous sulphate Nutrition 0.000 description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 3
- 239000011565 manganese chloride Substances 0.000 description 3
- 235000002867 manganese chloride Nutrition 0.000 description 3
- 229940099607 manganese chloride Drugs 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 description 3
- 229960001763 zinc sulfate Drugs 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a fermentation reactor, and discloses a fermentation reactor for coenzyme Q10 and application thereof. The fermentation reactor comprises a tubular reactor body (1), a heating structure (2) arranged outside the tubular reactor body (1) and an air inlet structure connected with the tubular reactor body (1), wherein the tubular reactor body (1) is spirally arranged and is provided withThe structure of the tubular reactor body (1) satisfies the following formula:formula (I); d=ar formula (II); wherein L is the screw pitch of the tubular reactor body (1), D is the spiral radius of the tubular reactor body (1), R is the inner tube diameter of the tubular reactor body (1), a is a coefficient, and a is 2-3. The fermentation reactor can effectively improve the fermentation efficiency of the coenzyme Q10 and ensure that the coenzyme Q10 has higher yield.
Description
Technical Field
The invention relates to a fermentation reactor, in particular to a fermentation reactor for coenzyme Q10 and application thereof.
Background
In the fields of bioengineering, pharmaceutical industry and the like, the fermentation process is an important production process. The traditional batch fermentation process has the defects of long reaction time, low yield, unstable quality and the like. To solve these problems, a continuous fermentation process has been developed.
The continuous fermentation process is a fermentation process which can realize long-time stable operation by continuously supplementing nutrient substances and controlling the discharge of waste materials in a certain time to keep the reaction system in a stable state. Compared with batch fermentation, continuous fermentation can improve the reaction rate and yield, reduce the waste treatment capacity and improve the control and stability of the reaction process.
In a continuous fermentation process, a continuous fermentation reactor is a critical device. Conventional continuous fermentation reactors typically employ tower or stirred reactors, but these reactors suffer from the disadvantages of complex operation, low equipment utilization, uneven fluid distribution, etc. To overcome these drawbacks, some new continuous fermentation reactors have been proposed, such as tubular continuous fermentation reactors, membrane continuous fermentation reactors, etc.
The commonly used tubular reactor is used for feeding the suspended solid fermentation raw material with high concentration from one end to the other end and discharging the suspended solid fermentation raw material from the other end, so that the tubular reactor can separate the inflow and outflow of the product, and is convenient for collecting and separating the reaction product. However, when the tube reactor is used for coenzyme Q10 fermentation, the fermentation efficiency thereof is still to be improved.
Disclosure of Invention
The invention aims to solve the problem that the fermentation efficiency of a tubular reactor for fermenting coenzyme Q10 needs to be improved in the prior art, and provides a fermentation reactor for fermenting coenzyme Q10 and application thereof.
The inventor unexpectedly found in the research process that the tubular reactor body is spirally arranged, and the spiral tubular reactor body can meet the formulas shown in the formulas (I) and (II), so that the fermentation reaction of the coenzyme Q10 can be promoted, the fermentation efficiency of the coenzyme Q10 can be effectively improved, and the coenzyme Q10 has higher yield.
In order to achieve the above object, an aspect of the present invention provides a fermentation reactor for coenzyme Q10, comprising a tubular reactor body, a heating structure provided outside the tubular reactor body, and an air intake structure connected to the tubular reactor body, the tubular reactor body being provided in a spiral shape, and the structure of the tubular reactor body satisfying the following formula:
formula (I);
d=ar formula (II);
wherein units of L, D and R are the same, L is a screw pitch of the tubular reactor body, D is a screw radius of the tubular reactor body, R is an inner tube diameter of the tubular reactor body, a is a coefficient, and a is 2-3.
Preferably, R is 8-10mm.
Preferably, the tubular reactor body is provided with a reactor feed inlet and a reactor discharge outlet, and the air inlet structure comprises an air inlet pipeline arranged at the reactor feed inlet and an air inlet arranged on the air inlet pipeline.
Further preferably, the air inlet pipe is provided in a circular ring shape, the plurality of air inlets are provided in a plurality on the outer peripheral wall of the air inlet pipe around the circumferential direction, the air inlets extend outward along the tangential direction of the air inlet pipe, and the plurality of air inlets are in the same plane.
Preferably, the heating structure is a heating sleeve, and a heat exchange liquid inlet and a heat exchange liquid outlet are arranged on the heating sleeve.
Preferably, the tubular reactor further comprises a control unit, wherein a temperature detection unit and a dissolved oxygen detection unit are arranged in the tubular reactor body, and the control unit is respectively connected with the temperature detection unit and the dissolved oxygen detection unit so as to receive information detected by the temperature detection unit and the dissolved oxygen detection unit and control the heating temperature of the heating structure and the air inflow of the air inlet structure according to the information.
Further preferably, a pH detection unit is further provided in the tubular reactor body, and the control unit is connected with the pH detection unit to receive information detected by the pH detection unit.
Preferably, the reactor further comprises a baffle structure, wherein the baffle structure comprises a baffle and a driving structure for driving the baffle to rotate, and the baffle is arranged in the tubular reactor body.
Further preferably, a plurality of baffles are provided in the tubular reactor body along the extending direction of the tubular reactor body, and the length of the baffles is gradually increased along the flowing direction of the material.
In a second aspect, the invention provides the use of a fermentation reactor according to the first aspect in a coenzyme Q10 fermentation process.
According to the technical scheme, the tubular reactor body is spirally arranged, and the relationship among the pitch, the spiral radius and the pipe diameter of the spiral tubular reactor body is further limited, so that the fermentation efficiency and the yield of the coenzyme Q10 can be remarkably improved in the fermentation process of the coenzyme Q10.
Other advantages and technical effects of the preferred embodiments of the present invention will be further described in the following detailed description.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention, illustrate and explain the invention and are not to be construed as limiting the invention. In the drawings:
FIG. 1 is a schematic view of a tubular reactor according to one embodiment of the present invention;
fig. 2 is a schematic structural view of an air intake structure according to an embodiment of the present invention.
Description of the reference numerals
1: a tubular reactor body; 2: a heating structure; 3: an air intake duct; 4: an air inlet; 5: a feed pipe; 6: and a discharging pipe.
Detailed Description
The following describes specific embodiments of the present invention in detail with reference to the drawings. It should be understood that the detailed description and specific examples, while indicating and illustrating the invention, are not intended to limit the invention.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; the connection may be direct, indirect via an intermediate medium, abutting, communication between two elements, or interaction between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
It is to be understood that the directions or positional relationships indicated by the terms "upper", "lower", "front", "rear", "inner", "outer", etc., are based on the directions or positional relationships shown in the drawings, are merely for convenience of description and to simplify the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the invention.
In one basic embodiment of the present invention, as shown in FIG. 1, there is provided a fermentation reactor for coenzyme Q10, comprising a tubular reactor body 1, a heating structure 2 provided outside the tubular reactor body 1 and an air intake structure connected to the tubular reactor body 1, the tubular reactor body 1 being provided in a spiral shape, and the structure of the tubular reactor body 1 satisfying the following formula:
L/2D is more than or equal to 0.1 and less than or equal to 0.25;
d=ar formula (II);
wherein units of L, D and R are the same, L is the screw pitch of the tubular reactor body 1, D is the screw radius of the tubular reactor body 1, R is the inner tube diameter of the tubular reactor body 1, and a is 2-3.
Specifically, the heating structure 2 may be any structure capable of heating the tubular reactor body 1, such as a heating jacket or a heating jacket, or the like. The air intake structure may be any structure capable of taking in air to the tubular reactor body 1, such as an air intake pipe or an air intake port provided in the tubular reactor body 1.
When the fermentation reactor provided by the basic embodiment is used, the seed fermentation liquid containing the coenzyme Q10 is injected into the fermentation reactor containing the fermentation medium with specific pH, the temperature of the fermentation medium in the fermentation reactor is regulated to be 28-32 ℃ through the heating structure 2, the oxygen content in the fermentation medium is regulated to be 30-40% through the air inlet structure, and then fermentation is carried out.
The inventors have unexpectedly found during the course of the study that by arranging the tubular reactor body in a spiral shape and restricting the structure of the spiral tubular reactor body to satisfy the formulas (I) and (II), the fermentation efficiency and yield of coenzyme Q10 can be significantly improved in the fermentation process for coenzyme Q10.
In one embodiment of the invention, R is 8-10mm. By limiting the inner tube diameter R of the tubular reactor body 1 to the above-described range, the fermentation efficiency and yield of coenzyme Q10 can be further improved.
In one embodiment of the invention, the tubular reactor body 1 is provided with a reactor feed inlet and a reactor discharge outlet, and the air inlet structure comprises an air inlet pipeline 3 arranged at the reactor feed inlet and an air inlet 4 arranged on the air inlet pipeline.
As a relatively preferred embodiment of the present invention, as shown in fig. 2, the intake duct 3 is provided in a circular ring shape, the intake ports 4 are provided in plurality, the plurality of intake ports 4 are provided on the outer peripheral wall of the intake duct 3 around the circumferential direction, the intake ports 4 extend outward in the tangential direction of the intake duct 3, and the plurality of intake ports 4 are in the same plane. By the arrangement, the oxygen content in the fermentation medium can be better controlled, so that the fermentation reaction can be promoted, and the fermentation efficiency and yield of the coenzyme Q10 can be improved.
In one embodiment of the present invention, the heating structure 2 is a heating jacket, and a heat exchange liquid inlet and a heat exchange liquid outlet are arranged on the heating jacket. Through the setting of heating structure 2, can evenly heat the inside liquid of heating tube reactor body 1 for the temperature in the heating tube reactor body 1 is even, makes it can satisfy the fermentation condition of coenzyme Q10 more easily.
In one embodiment of the present invention, the tubular reactor further comprises a control unit, wherein a temperature detection unit and a dissolved oxygen detection unit are arranged in the tubular reactor body 1, and the control unit is respectively connected with the temperature detection unit and the dissolved oxygen detection unit to receive information detected by the temperature detection unit and the dissolved oxygen detection unit and control the heating temperature of the heating structure 2 and the air inflow of the air inlet structure according to the information.
According to the present invention, the temperature detecting unit and the dissolved oxygen detecting unit are provided in the tubular reactor body 1 and can be brought into contact with or placed in the culture medium during use, preferably, the temperature detecting unit and the dissolved oxygen detecting unit can be placed in the culture medium during use to detect the temperature and the dissolved oxygen amount of the culture medium.
The temperature detection unit may be any unit that can be used to detect temperature, such as a temperature sensor or various units containing a temperature sensor. The dissolved oxygen detection unit may be any unit that can be used to detect the amount of dissolved oxygen, such as a dissolved oxygen sensor or various units containing a dissolved oxygen sensor. The intake structure may include an intake valve, and the intake air amount of the intake structure is controlled by controlling the intake valve. The control of the heating temperature of the heating structure 2 may be achieved by controlling the temperature of the heat exchanging liquid in the heating structure 2, whereas the control of the temperature of the heat exchanging liquid in the heating structure 2 may be achieved by methods disclosed in the prior art.
The carrier of the control unit is a computer, and the control program in the control unit is in the prior art.
Through the arrangement, the temperature and dissolved oxygen amount of the fermentation medium in the fermentation reactor can be accurately controlled, so that the fermentation reaction is more sufficient, and the fermentation efficiency and yield of the coenzyme Q10 are further improved.
In one embodiment of the present invention, a pH detecting unit is further provided in the tubular reactor body 1, and the control unit is connected to the pH detecting unit to receive information detected by the pH detecting unit.
Specifically, a pH detection unit is provided inside the tubular reactor body 1, and is capable of detecting the pH of the fermentation medium inside the reactor body 1. The pH detection unit may be a pH sensor or a unit containing a pH sensor. Through the arrangement, the pH value of the fermentation medium in the fermentation reactor can be accurately controlled, so that the fermentation reaction is more sufficient, and the fermentation efficiency and yield of the coenzyme Q10 are further improved.
As a specific embodiment of the present invention, a baffle structure is further included, the baffle structure including a baffle and a driving structure for driving the baffle to rotate, the baffle being disposed in the tubular reactor body 1.
The drive structure may be a drive motor, a drive shaft connected between the drive motor and the barrier, or a drive string. The fermentation culture medium can be stirred through the rotation or sliding of the baffle plate, so that the dissolved oxygen amount in the fermentation culture medium is adjusted, the fermentation reaction is more sufficient, and the fermentation efficiency and yield of the coenzyme Q10 are further improved.
In one embodiment of the present invention, a plurality of baffles are provided in the tubular reactor body 1 along the extending direction of the tubular reactor body 1, and the length of the baffles is gradually increased along the flowing direction of the material. The length of the baffle plate is gradually increased, so that the stirring effect of the baffle plate on the fermentation medium can be improved, and further the fermentation reaction is more sufficient.
In one embodiment of the invention, the reactor further comprises a feeding pipe 5 and a discharging pipe 6, wherein the feeding pipe 5 is connected with the feeding hole of the tubular reactor body 1, and the discharging pipe 6 is connected with the discharging hole of the reactor body 1.
As a relatively preferred embodiment of the present invention, as shown in fig. 1 and 2, there is provided a fermentation reactor for coenzyme Q10, comprising a tubular reactor body 1, a heating structure 2, an air intake structure, a control unit, a temperature detecting unit, a dissolved oxygen detecting unit, a pH detecting unit and a baffle structure, the heating structure 2 being a heating jacket provided outside the tubular reactor body 1, the heating jacket being provided with a heat exchange liquid inlet and a heat exchange liquid outlet; the tubular reactor body 1 is provided in a spiral shape, and the structure of the tubular reactor body 1 satisfies the following formula:
formula (I);
d=ar formula (II);
wherein units of L, D and R are the same, L is the screw pitch of the tubular reactor body 1, D is the spiral radius of the tubular reactor body 1, R is the inner tube diameter of the tubular reactor body 1, a is a coefficient, a is 2-3, and R is 8-10mm; the tubular reactor comprises a tubular reactor body 1, a reactor feed inlet and a reactor discharge outlet, wherein an air inlet structure comprises an air inlet pipeline 3 arranged at the reactor feed inlet and an air inlet 4 arranged on the air inlet pipeline 3, the air inlet pipeline 3 is in a circular ring shape, the air inlet 4 is provided with a plurality of air inlets 4, the plurality of air inlets 4 are circumferentially arranged on the peripheral wall of the air inlet pipeline 3, the air inlets 4 extend outwards along the tangential direction of the air inlet pipeline 3, and the plurality of air inlets 4 are in the same plane; the temperature detection unit, the dissolved oxygen detection unit, the pH detection unit and the baffle structure are arranged in the tubular reactor body 1 and can be in contact with the fermentation medium in the tubular reactor body 1, and the control unit is respectively connected with the temperature detection unit, the dissolved oxygen detection unit and the pH detection unit to receive information detected by the detection units and control the heating temperature of the heating structure 2, the air inflow of the air inlet structure and the stirring speed of the baffle structure according to the information; the baffle structure includes baffle and is used for driving baffle pivoted drive structure, and the baffle setting is provided with a plurality ofly in tubular reactor body 1, and a plurality of baffles set up in tubular reactor body 1 along the extending direction of tubular reactor body 1, and along the flow direction of material, the length of baffle increases gradually.
The fermentation reactor provided by the preferred embodiment can improve the yield of fermentation by about 30% and the biomass by about 50%. But also can be automatically controlled and produced in large scale, and has wide application prospect.
In addition, the invention also provides an application of the fermentation reactor provided by any embodiment in the coenzyme Q10 fermentation process.
Specifically, rhodobacter sphaeroides (Rhodobacter sphaeroides) are inoculated into a seed culture medium according to an inoculum size of 1-5% (m/v), and are cultured for 12-48 hours to obtain a seed culture solution; and (3) placing the fermentation medium with the seed culture solution into the tubular reactor provided by any embodiment for fermentation, wherein the seed culture solution is inoculated into the fermentation culture according to an inoculation amount of 5-10% (v/v), and the temperature of the fermentation medium is controlled to be 28-32 ℃, the dissolved oxygen amount is controlled to be 30-40% and the pH value is controlled to be 6.5-8.5 in the whole fermentation reaction process.
The seed medium and the fermentation medium may be determined by a tester according to actual conditions, and preferably the seed medium includes: 4-6g/L glucose, 8-12g/L tryptone, 4-6g/L soybean peptone, 3-5g/L sodium chloride and 1-3g/L potassium dihydrogen phosphate. The fermentation medium comprises: 42-46g/L molasses, 15-25g/L sucrose, 30-40g/L corn steep liquor dry powder, 54-58g/L soybean peptone, 1-3g/L ammonium sulfate, 1-3g/L potassium dihydrogen phosphate, 0.1-0.3 mass% trace elements and 0.01-0.03 mass% biotin. Wherein the trace elements may include iron, calcium, manganese, zinc, copper, cobalt and sodium; biotin may include riboflavin, niacin, vitamin b2, and thiamine. The proportion of each element in the microelements can be determined according to the actual situation, and the proportion of flavin, nicotinic acid, vitamin b2 and thiamine in the biotin can also be determined according to the actual situation.
The present invention will be described in detail by examples. In the examples below, rhodobacter sphaeroides are commercially available. High performance liquid chromatography was purchased from japanese Hitachi.
Tubular reactor 1
The device comprises a tubular reactor body 1, a heating structure 2, an air inlet structure, a control unit, a temperature detection unit, a dissolved oxygen detection unit, a pH detection unit and a baffle structure, wherein the heating structure 2 is a heating sleeve, the heating sleeve is arranged outside the tubular reactor body 1, and a heat exchange liquid inlet and a heat exchange liquid outlet are arranged on the heating sleeve; the tubular reactor body 1 is provided in a spiral shape, and the structure of the tubular reactor body 1 satisfies the following formula:
；D=2.5R；
wherein L is the screw pitch of the tubular reactor body 1, D is the spiral radius of the tubular reactor body 1, R is the inner pipe diameter of the tubular reactor body 1, R is 10mm, the pipe length is 0.8m, the pipe wall thickness is 2mm, and the coiling angle is 11.3 degrees; the tubular reactor comprises a tubular reactor body 1, a reactor feed inlet and a reactor discharge outlet, wherein an air inlet structure comprises an air inlet pipeline 3 arranged at the reactor feed inlet and air inlets 4 arranged on the air inlet pipeline 3, the air inlet pipeline 3 is in a circular ring shape, the number of the air inlets 4 is eight, the eight air inlets 4 are circumferentially arranged on the peripheral wall of the air inlet pipeline 3, the air inlets 4 extend outwards along the tangential direction of the air inlet pipeline 3, and the eight air inlets 4 are in the same plane; the temperature detection unit, the dissolved oxygen detection unit, the pH detection unit and the baffle structure are arranged in the tubular reactor body 1 and can be in contact with the fermentation medium in the tubular reactor body 1, and the control unit is respectively connected with the temperature detection unit, the dissolved oxygen detection unit and the pH detection unit to receive information detected by the detection units and control the heating temperature of the heating structure 2, the air inflow of the air inlet structure and the stirring speed of the baffle structure according to the information; the baffle structure includes baffle and is used for driving baffle pivoted drive structure, and the baffle setting is in tubular reactor body 1, and the baffle is provided with four, and four baffles set up in tubular reactor body 1 along the extending direction of tubular reactor body 1, and along the flow direction of material, the length of baffle increases gradually.
Tubular reactor 2
With a tubeThe difference of the device 1 is that:the method comprises the steps of carrying out a first treatment on the surface of the D=4 r, r is 10mm.
Example 1
(1) Inoculating rhodobacter sphaeroides into a seed culture medium according to an inoculum size of 3% (m/v), and culturing for 24 hours at a temperature of 28-32 ℃ to obtain a seed culture solution;
the seed medium comprises: 5g/L glucose, 10g/L tryptone, 5g/L soytone, 4g/L sodium chloride and 2g/L potassium dihydrogen phosphate;
(2) Inoculating the seed culture solution into fermentation culture according to the inoculum size of 8% (v/v), then injecting the fermentation culture medium into the tubular reactor 1 at the speed of 4mL/h for fermentation reaction, controlling the temperature to be 28-32 ℃ in the fermentation process, controlling the dissolved oxygen to be 35+/-2% and controlling the pH to be 7.5+/-0.5, and fermenting for 48 hours to obtain a fermentation product;
the fermentation medium comprises: 44g/L molasses, 20g/L sucrose, 35g/L corn steep liquor dry powder, 56g/L soybean peptone, 2g/L ammonium sulfate, 2g/L potassium dihydrogen phosphate, 0.5g/L ferrous sulfate, 0.5g/L calcium chloride, 0.5g/L manganese chloride, 0.2g/L zinc sulfate, 0.15g/L copper sulfate, 0.15g/L cobalt chloride, 5g/L EDTA-Na and 0.02 mass% biotin, in which the mass ratio of riboflavin, niacin, vitamin b2 and thiamine is 1:1:1:1.
Example 2
(1) Inoculating rhodobacter sphaeroides into a seed culture medium according to an inoculum size of 1% (m/v), and culturing for 24 hours at a temperature of 28-32 ℃ to obtain a seed culture solution;
the seed medium comprises: 4g/L glucose, 12g/L tryptone, 4g/L soytone, 3g/L sodium chloride and 1g/L potassium dihydrogen phosphate;
(2) Inoculating the seed culture solution into fermentation culture according to an inoculum size of 10% (v/v), then injecting the fermentation culture medium into the tubular reactor 1 at a speed of 2mL/h for fermentation reaction, controlling the temperature to be 28-32 ℃ and the dissolved oxygen to be 32+/-2% and the pH to be 8+/-0.5 in the fermentation process, and fermenting for 48 hours to obtain a fermentation product;
the fermentation medium comprises: 42g/L molasses, 25g/L sucrose, 30g/L corn steep liquor dry powder, 58g/L soybean peptone, 1g/L ammonium sulfate, 3g/L potassium dihydrogen phosphate, 0.5g/L ferrous sulfate, 0.5g/L calcium chloride, 0.5g/L manganese chloride, 0.2g/L zinc sulfate, 0.15g/L copper sulfate, 0.15g/L cobalt chloride, 5g/L EDTA-Na and 0.02 mass% biotin, in which the mass ratio of riboflavin, niacin, vitamin b2 and thiamine is 1:1:1:1.
Example 3
(1) Inoculating rhodobacter sphaeroides into a seed culture medium according to an inoculum size of 5% (m/v), and culturing for 24 hours at a temperature of 28-32 ℃ to obtain a seed culture solution;
the seed medium comprises: 6g/L glucose, 8g/L tryptone, 6g/L soy peptone, 5g/L sodium chloride and 4g/L potassium dihydrogen phosphate;
(2) Inoculating the seed culture solution into fermentation culture according to an inoculum size of 5% (v/v), then injecting the fermentation culture medium into the tubular reactor 1 at a speed of 6mL/h for fermentation reaction, controlling the temperature to be 28-32 ℃ and the dissolved oxygen to be 38+/-2% and the pH to be 7+/-0.5 in the fermentation process, and fermenting for 48 hours to obtain a fermentation product;
the fermentation medium comprises: 46g/L molasses, 15g/L sucrose, 40g/L corn steep liquor dry powder, 54g/L soybean peptone, 3g/L ammonium sulfate, 1g/L potassium dihydrogen phosphate, 0.5g/L ferrous sulfate, 0.5g/L calcium chloride, 0.5g/L manganese chloride, 0.2g/L zinc sulfate, 0.15g/L copper sulfate, 0.15g/L cobalt chloride, 5g/L EDTA-Na and 0.02 mass% biotin, in which the mass ratio of riboflavin, niacin, vitamin b2 and thiamine is 1:1:1:1.
Comparative example 1
The fermentation was carried out as described in example 2, except that the tubular reactor used was tubular reactor 2.
Comparative example 2
The fermentation was carried out as described in example 3, except that the tubular reactor used was tubular reactor 2.
Test case
The fermentation products obtained in examples and comparative examples were centrifuged at 8000rpm for 10min, the supernatant was filtered through a 0.45 μm filter to analyze the crude product yield of coenzyme Q10, 20mL of the sample was subjected to HPLC detection, the mobile phase was formic acid/n-hexane, and the coenzyme Q10 content was detected by ultraviolet at 275nm, and the obtained parameters were shown in Table 1. The biomass of the fermentation was converted by measuring the optical density at 600nm using a visible light spectrophotometer, and the standard curve of absorbance was obtained when the fermentation broth was diluted in proportion, and the obtained parameters were shown in table 1.
TABLE 1
As can be seen from Table 1, the fermentation reactor provided by the invention can promote the fermentation reaction of coenzyme Q10, effectively improve the fermentation efficiency of coenzyme Q10 and enable the coenzyme Q10 to have higher yield. The output of coenzyme Q10 is improved by about 30 percent, and the biomass content is improved by about 50 percent.
The preferred embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Moreover, any combination of the various embodiments of the invention can be made without departing from the spirit of the invention, which should also be considered as disclosed herein.
Claims (8)
1. A fermentation reactor for coenzyme Q10, characterized by comprising a tubular reactor body (1), a heating structure (2) arranged outside the tubular reactor body (1) and an air inlet structure connected with the tubular reactor body (1), wherein the tubular reactor body (1) is arranged in a spiral shape, and the structure of the tubular reactor body (1) satisfies the following formula:
formula (I);
d=ar formula (II);
wherein units of L, D and R are the same, L is a screw pitch of the tubular reactor body (1), D is a screw radius of the tubular reactor body (1), R is an inner tube diameter of the tubular reactor body (1), a is a coefficient, and a is 2-3;
the reactor comprises a tubular reactor body (1), and is characterized in that a reactor feed inlet and a reactor discharge outlet are formed in the tubular reactor body (1), the air inlet structure comprises an air inlet pipeline (3) arranged at the reactor feed inlet and an air inlet (4) arranged on the air inlet pipeline (3), the air inlet pipeline (3) is arranged in a circular shape, a plurality of air inlets (4) are arranged on the peripheral wall of the air inlet pipeline (3) around the circumferential direction, the air inlets (4) extend outwards along the tangential direction of the air inlet pipeline (3), and a plurality of air inlets (4) are arranged in the same plane.
2. The fermentation reactor of claim 1, wherein R is 8-10mm.
3. Fermentation reactor according to claim 1 or 2, characterized in that the heating structure (2) is a heating jacket, on which a heat exchange liquid inlet and a heat exchange liquid outlet are arranged.
4. Fermentation reactor according to claim 1 or 2, further comprising a control unit, wherein a temperature detection unit and a dissolved oxygen detection unit are arranged in the tubular reactor body (1), the control unit being connected to the temperature detection unit and the dissolved oxygen detection unit, respectively, for receiving information detected by the temperature detection unit and the dissolved oxygen detection unit and for controlling the heating temperature of the heating structure (2) and the air intake amount of the air intake structure according to the above information.
5. Fermentation reactor according to claim 4, characterized in that a pH detection unit is also provided in the tubular reactor body (1), the control unit being connected to the pH detection unit for receiving information detected by the pH detection unit.
6. A fermentation reactor according to claim 1 or 2, further comprising a baffle structure comprising a baffle and a drive structure for driving the baffle in rotation, the baffle being arranged in the tubular reactor body (1).
7. A fermentation reactor according to claim 6, wherein a plurality of baffles are provided, a plurality of baffles being provided in the tubular reactor body (1) along the extension direction of the tubular reactor body (1) and the length of the baffles gradually increasing along the flow direction of the material.
8. Use of a fermentation reactor according to any one of claims 1 to 7 in a coenzyme Q10 fermentation process.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103255051A (en) * | 2013-05-09 | 2013-08-21 | 江苏丰泽生物工程设备制造有限公司 | Tubular circulating bioreactor and tubular circulating biological reaction system device |
| CN209442980U (en) * | 2018-10-10 | 2019-09-27 | 南京和润隆环保科技有限公司 | A kind of plug-flow reactor |
| CN110951821A (en) * | 2019-12-06 | 2020-04-03 | 黑龙江八一农垦大学 | Rhodobacter sphaeroides mutant strain for producing coenzyme Q10 and method for producing coenzyme Q10 through fermentation |
| CN210394325U (en) * | 2019-07-15 | 2020-04-24 | 上海益恺生物科技有限公司 | Preparation facilities of coenzyme Q10 based on biological fermentation technique |
| CN117025363A (en) * | 2023-09-19 | 2023-11-10 | 云南师范大学 | Anaerobic digestion system and anaerobic fermentation gas preparation method |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103255051A (en) * | 2013-05-09 | 2013-08-21 | 江苏丰泽生物工程设备制造有限公司 | Tubular circulating bioreactor and tubular circulating biological reaction system device |
| CN209442980U (en) * | 2018-10-10 | 2019-09-27 | 南京和润隆环保科技有限公司 | A kind of plug-flow reactor |
| CN210394325U (en) * | 2019-07-15 | 2020-04-24 | 上海益恺生物科技有限公司 | Preparation facilities of coenzyme Q10 based on biological fermentation technique |
| CN110951821A (en) * | 2019-12-06 | 2020-04-03 | 黑龙江八一农垦大学 | Rhodobacter sphaeroides mutant strain for producing coenzyme Q10 and method for producing coenzyme Q10 through fermentation |
| CN117025363A (en) * | 2023-09-19 | 2023-11-10 | 云南师范大学 | Anaerobic digestion system and anaerobic fermentation gas preparation method |
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