CN104403937B - Rotate internal circulation gas-lift type membrane bioreactor and process system thereof - Google Patents

Rotate internal circulation gas-lift type membrane bioreactor and process system thereof Download PDF

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CN104403937B
CN104403937B CN201410788068.0A CN201410788068A CN104403937B CN 104403937 B CN104403937 B CN 104403937B CN 201410788068 A CN201410788068 A CN 201410788068A CN 104403937 B CN104403937 B CN 104403937B
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叶立强
李倩
冯宝民
姚子昂
吴海歌
崔红利
董娟
陈琼
余雅娴
钟林杉
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Abstract

本发明公开了一种旋转内循环气升式膜生物反应器,包括反应釜主体、一级膜分离装置和滤液收集装置;一级膜组件均布在导流筒的外圆壁四周,一级膜组件的两端分别可拆卸式固定在上下排布的两个旋转架上,旋转架固定在密封轴承上,密封轴承套装在导流筒上;滤液收集装置包括滤液收集器和滤液收集管;滤液收集器内可根据工艺选择安装二级膜组件。旋转内循环气升式膜生物反应器集气升反应与膜分离于一体,整个过程所需的能量大都由压缩气体提供,膜组件数量、角度、启用与否、膜通量都可以人工进行调节,以适应气升式反应器的不同操作,很好的实现了二者的耦合,具有能耗低、结构简单、操作灵活、反应条件温和等优点。

The invention discloses a rotating internal circulation air-lift membrane bioreactor, which comprises a reactor main body, a primary membrane separation device and a filtrate collection device; The two ends of the membrane module are detachably fixed on the two rotating frames arranged up and down, the rotating frame is fixed on the sealed bearing, and the sealed bearing is set on the guide tube; the filtrate collecting device includes a filtrate collector and a filtrate collecting pipe; Secondary membrane modules can be installed in the filtrate collector according to the process. The rotating internal circulation airlift membrane bioreactor integrates airlift reaction and membrane separation. Most of the energy required for the whole process is provided by compressed gas. The number, angle, activation or not of membrane modules, and membrane flux can all be adjusted manually. , in order to adapt to the different operations of the airlift reactor, the coupling of the two is well realized, and it has the advantages of low energy consumption, simple structure, flexible operation, and mild reaction conditions.

Description

旋转内循环气升式膜生物反应器及其工艺系统Rotating internal circulation airlift membrane bioreactor and its process system

技术领域technical field

本发明涉及膜生物反应器技术领域,尤其涉及一种旋转内循环气升式膜生物反应器及其工艺系统。The invention relates to the technical field of membrane bioreactors, in particular to a rotary internal circulation airlift membrane bioreactor and a process system thereof.

背景技术Background technique

在生化反应过程中,随着反应进行,发酵产物不断积累,产物抑制作用逐渐突出,如在大众化学品如乙醇生产中,对一般酿酒酵母,单级罐批次发酵末期乙醇浓度达到18%-20%时菌体活性严重被抑制甚至出现菌体大量死亡。在酶法降解生物多糖制备活性寡糖的过程中,胞外酶往往受底物诱导和产物抑制双重作用,如卡拉胶或褐藻胶生产中,底物被酶切获得的二糖单位过多积累会影响菌体继续产酶。In the process of biochemical reaction, as the reaction proceeds, the fermentation product accumulates continuously, and the inhibitory effect of the product gradually becomes prominent. For example, in the production of popular chemicals such as ethanol, for general Saccharomyces cerevisiae, the concentration of ethanol at the end of single-stage tank batch fermentation reaches 18%- At 20%, the cell activity was severely inhibited and even a large number of cells died. In the process of enzymatically degrading biological polysaccharides to prepare active oligosaccharides, extracellular enzymes are often subject to both substrate induction and product inhibition. For example, in the production of carrageenan or alginate, excessive accumulation of disaccharide units obtained by enzymatic cleavage of substrates It will affect the bacteria to continue to produce enzymes.

另一方面,产物消耗作用也经常存在,如灵芝多糖深层发酵过程中,发酵所得的灵芝多糖可以被发酵菌体利用,不及时移除系统会造成收率极大的降低。On the other hand, product consumption also often exists. For example, during the submerged fermentation of Ganoderma lucidum polysaccharides, the fermented Ganoderma lucidum polysaccharides can be used by the fermentation cells, and the yield will be greatly reduced if the system is not removed in time.

因此,为了提高反应得率和生产强度,利用膜生物反应器耦合反应与分离操作的特性,来“边反应边移走”体系中的产物是很好的解决方案之一。这类生物反应器将微生物发酵和产物分离相结合,常用于解决发酵产物抑制、底物选择供给等问题,通过长时间的连续发酵,达到微生物的高密度生长和增加发酵产物产率的目的,细胞密度或代谢产物产率通常可以达到间歇发酵的十倍甚至一百倍以上。在该过程中,发酵液进过膜过滤后,将发酵产物有效地移出发酵罐,减轻甚至消除了产物的抑制和消耗作用,而剩余的残液与被截留的细胞继续返回发酵罐中,从而保持了发酵罐中的高细胞浓度,同时很方便的实现了罐内液位平衡。例如采用膜生物反应器系统进行灵芝多糖的连续发酵,可溶性灵芝多糖可被连续渗透通过膜生物反应器的滤膜。通过连续补充新鲜料液,改善了糖源,减少了菌丝体对多糖的利用,提高了菌丝体的得率和灵芝多糖的产量,菌丝体生长则随稀释率的增大而增大,总的菌丝体干重可比间歇发酵增加20%以上。Therefore, in order to improve the reaction yield and production intensity, it is one of the good solutions to use the characteristics of membrane bioreactor coupling reaction and separation operation to "remove while reacting" the product in the system. This type of bioreactor combines microbial fermentation and product separation, and is often used to solve problems such as fermentation product inhibition and substrate selection and supply. Through long-term continuous fermentation, it can achieve the purpose of high-density growth of microorganisms and increase the yield of fermentation products. The cell density or the yield of metabolites can usually reach ten times or even more than one hundred times that of batch fermentation. In this process, after the fermentation liquid is filtered through the membrane, the fermentation product is effectively removed from the fermenter, reducing or even eliminating the inhibition and consumption of the product, while the remaining raffinate and trapped cells continue to return to the fermenter, thereby The high cell concentration in the fermenter is maintained, and at the same time, the liquid level balance in the tank is conveniently realized. For example, using a membrane bioreactor system for continuous fermentation of Ganoderma lucidum polysaccharides, soluble Ganoderma lucidum polysaccharides can be continuously permeated through the filter membrane of the membrane bioreactor. By continuously replenishing fresh feed liquid, the sugar source is improved, the utilization of polysaccharide by mycelium is reduced, the yield of mycelium and the yield of Ganoderma lucidum polysaccharide are increased, and the growth of mycelium increases with the increase of dilution rate , the total dry weight of mycelium can be increased by more than 20% compared with batch fermentation.

但在膜反应器运行过程中,料液经泵输送之后在膜表面进行分离,膜的污染极为严重,而经过膜分离出来的产物浓度与反应罐中产物浓度相同,后续处理比较麻烦;同时,由于泵的使用,该过程能耗较高,而且流体的湍动剧烈容易恶化反应条件,不利于菌体生长。However, during the operation of the membrane reactor, the feed liquid is separated on the surface of the membrane after being transported by the pump, and the pollution of the membrane is extremely serious, and the concentration of the product separated by the membrane is the same as that in the reaction tank, and the follow-up treatment is more troublesome; at the same time, Due to the use of pumps, the energy consumption of this process is high, and the violent turbulence of the fluid is easy to deteriorate the reaction conditions, which is not conducive to the growth of bacteria.

为了克服这些问题,ZL01108189.9、ZL200520075139.9、ZL200910026442.2先后提出了以压缩气体作为膜生物反应器的动力,取代传统的泵。但是前者通过普通管道将反应器与膜组件连接,致使反应器结构较为分散,流体循环线路较长,从而使得气液传质效果受到影响,较难满足大规模反应的需求。中者在前者的基础上提出了外环流气升式膜反应器,在该反应器中,膜组件与反应器很好的耦合,但是在该反应器中回流口位于反应釜体上部的扩大段处,回流口位于液面之上或者位于液面下的深度很小,同时回流口处没有导流管,大量压缩气体在循环结束后立即逸出排掉,只起到了循环动力的作用,未得到合理利用,造成能量损失。后者在前两者的基础上提出了外环流气提式膜生物反应器,该反应器在不改变气升式反应器基本结构的基础上引进膜组件,同时具备气升式反应器、膜生物反应器和气提的功能。但该反应器中气提单元的存在,限制了该反应器的应用范围,即对抑制性产物有挥发特性的要求。同时,由于该类反应器的膜组件位于循环管内,增加了气升式反应器的循环阻力,容易造成反应釜内细胞与营养成分分布不均匀。上述这些发明在一定程度解决了膜反应器反应单元-分离单元耦合的问题,但为了进行耦合引入了其他复杂结构而不利于放大,且存在能源利用率低、适用范围较窄、结构复杂、膜易受污染等问题和缺陷。In order to overcome these problems, ZL01108189.9, ZL200520075139.9, and ZL200910026442.2 successively proposed to use compressed gas as the power of the membrane bioreactor instead of the traditional pump. However, the former connects the reactor and the membrane module through ordinary pipes, resulting in a relatively dispersed reactor structure and a long fluid circulation line, which affects the gas-liquid mass transfer effect and is difficult to meet the needs of large-scale reactions. On the basis of the former, the latter proposes an external circulation air-lift membrane reactor. In this reactor, the membrane module is well coupled with the reactor, but in this reactor, the return port is located in the enlarged section of the upper part of the reactor body. At the point where the return port is located above or below the liquid surface, the depth is very small. At the same time, there is no guide tube at the return port, and a large amount of compressed gas will escape and be discharged immediately after the cycle ends, which only plays the role of cycle power and has no effect. Be rationally utilized, resulting in energy loss. The latter proposes an outer loop airlift membrane bioreactor on the basis of the former two, which introduces membrane modules without changing the basic structure of the airlift reactor. Function of bioreactor and gas stripping. However, the existence of the air stripping unit in the reactor limits the scope of application of the reactor, that is, the volatile characteristics of the suppressed product are required. At the same time, because the membrane module of this type of reactor is located in the circulation pipe, the circulation resistance of the airlift reactor is increased, and it is easy to cause uneven distribution of cells and nutrients in the reactor. The above-mentioned inventions have solved the problem of membrane reactor reaction unit-separation unit coupling to a certain extent, but for the purpose of coupling, other complex structures have been introduced, which is not conducive to amplification, and there are low energy efficiency, narrow application range, complex structure, membrane Susceptibility to problems and defects such as contamination.

由此可见,开发出一种新型的应用范围广阔的一体式膜生物反应器,特别是开发出一种适用于发酵领域的一体式膜生物反应器,将膜分离与气升式反应器的优势同时发挥出来,并具有高能量利用效率,具有十分重要的现实意义。It can be seen that a new type of integrated membrane bioreactor with a wide range of applications has been developed, especially an integrated membrane bioreactor suitable for the field of fermentation, which combines the advantages of membrane separation and airlift reactors. At the same time, it has high energy utilization efficiency and has very important practical significance.

发明内容Contents of the invention

本发明的目的是:为了解决上述在发酵领域所存在的产物抑制和产物消耗作用问题,根据膜分离反应器和气升式反应器的优势以及它们所具有的互相弥补缺点的特征,而提供一种旋转内循环气升式膜生物反应器及其工艺系统。The purpose of the present invention is: in order to solve the above-mentioned problems of product inhibition and product consumption in the field of fermentation, according to the advantages of membrane separation reactors and airlift reactors and the characteristics of their mutual compensation of shortcomings, provide a kind of A rotary internal circulation airlift membrane bioreactor and its process system.

为解决上述技术问题,本发明的技术方案是:旋转内循环气升式膜生物反应器,包括反应釜主体、一级膜分离装置和滤液收集装置;反应釜主体包括导气管、导流筒、釜体和气体分布器,釜体的顶部安装有压力表并设有进液口、排气口、通气口,排气口与排气控制阀相连,通气口与通气阀相连,釜体的外圆壁上设有视窗、循环液入口,釜体的底部设有排液口,釜体的内部安装有导流筒,导流筒的内部安装有导气管,导气管的上部与通气口相连,下部与气体分布器相连,气体分布器为阶梯多环形;一级膜分离装置包括旋转架、密封轴承和一级膜组件,一级膜组件为马蹄形截面的弯管式中空结构,若干根所述的一级膜组件均布在导流筒的外圆壁四周,一级膜组件的两端分别可拆卸式固定在上下排布的两个旋转架上,旋转架固定在密封轴承上,密封轴承套装在导流筒上;滤液收集装置包括滤液收集器和滤液收集管,滤液收集器包括密封轴承、上旋盖和罐体,上旋盖与罐体连接,密封轴承套装在上旋盖上,滤液收集器通过上旋盖安装在釜体的底部且位于气体分布器的正下方,滤液收集器与位于下部的旋转架通过滤液收集管相连,罐体的底部设有滤液口,滤液口与真空控制阀相连,罐体外圆壁的下部设有循环液出口,循环液出口与循环调节阀相连。In order to solve the above technical problems, the technical solution of the present invention is: a rotary internal circulation airlift membrane bioreactor, comprising a reactor main body, a primary membrane separation device and a filtrate collection device; The kettle body and the gas distributor, the top of the kettle body is equipped with a pressure gauge and is provided with a liquid inlet, an exhaust port, and a vent port. The exhaust port is connected to the exhaust control valve, and the vent port is connected to the vent valve. The round wall is equipped with a window and a circulating liquid inlet, and the bottom of the kettle body is provided with a liquid discharge port. The inside of the kettle body is equipped with a guide tube, and the inside of the guide tube is installed with an air guide tube. The upper part of the air guide tube is connected to the vent. The lower part is connected with the gas distributor, and the gas distributor is a stepped multi-ring; the first-stage membrane separation device includes a rotating frame, a sealed bearing and a first-stage membrane module. The first-stage membrane modules are evenly distributed around the outer circular wall of the guide tube, and the two ends of the first-stage membrane module are detachably fixed on two rotating frames arranged up and down. The rotating frame is fixed on the sealed bearing, and the sealed bearing Set on the guide tube; the filtrate collection device includes a filtrate collector and a filtrate collection pipe, the filtrate collector includes a sealed bearing, an upper screw cap and a tank body, the upper screw cap is connected with the tank body, and the sealed bearing is set on the upper screw cap, The filtrate collector is installed on the bottom of the kettle through the upper screw cap and is located directly below the gas distributor. The filtrate collector is connected to the rotating frame at the lower part through the filtrate collection pipe. The bottom of the tank is provided with a filtrate port, which is connected to the vacuum The control valve is connected, and the lower part of the outer wall of the tank is provided with a circulating fluid outlet, and the circulating fluid outlet is connected with the circulation regulating valve.

进一步的,滤液收集器内安装有二级膜组件。Further, a secondary membrane module is installed in the filtrate collector.

进一步的,釜体的外圆壁上开设有视窗。Further, a viewing window is provided on the outer circular wall of the kettle body.

进一步的,两个旋转架的间距为导流筒长度的0.1-1。Further, the distance between the two rotating frames is 0.1-1 of the length of the guide tube.

进一步的,反应釜主体的高径比为3-10。Further, the aspect ratio of the main body of the reactor is 3-10.

进一步的,一级膜组件的倾斜角度为20-90度。Further, the inclination angle of the primary membrane module is 20-90 degrees.

进一步的,釜体的侧面安装有温度计、pH计、溶氧电极、液位探头并设有取样口。Further, a thermometer, a pH meter, a dissolved oxygen electrode, a liquid level probe and a sampling port are installed on the side of the kettle body.

进一步的,滤液收集器与位于下部的旋转架通过3根滤液收集管呈120度间距相连。Further, the filtrate collector is connected to the lower rotating frame through 3 filtrate collecting pipes at an interval of 120 degrees.

旋转内循环气升式膜生物反应器的工艺系统,旋转内循环气升式膜生物反应器的通气阀依次与气体流量计、钢瓶、压缩空气机相连;旋转内循环气升式膜生物反应器的真空控制阀依次与滤液收集罐、真空泵相连;旋转内循环气升式膜生物反应器的循环调节阀依次与循环泵、旋转内循环气升式膜生物反应器的循环液入口相连。The process system of the rotary internal circulation airlift membrane bioreactor, the ventilation valve of the rotary internal circulation airlift membrane bioreactor is connected with the gas flow meter, the steel cylinder and the compressed air machine in turn; the rotary internal circulation airlift membrane bioreactor The vacuum control valve of the rotary internal circulation airlift membrane bioreactor is connected with the filtrate collection tank and the vacuum pump in sequence; the circulation regulating valve of the rotary internal circulation airlift membrane bioreactor is connected with the circulation pump and the circulating liquid inlet of the rotary internal circulation airlift membrane bioreactor in sequence.

进一步的,旋转内循环气升式膜生物反应器的滤液收集器内安装有二级膜组件。Further, a secondary membrane module is installed in the filtrate collector of the rotating internal circulation airlift membrane bioreactor.

进一步的,气体流量计依次与反冲控制阀、真空控制阀相连。Further, the gas flow meter is connected with the recoil control valve and the vacuum control valve in sequence.

本发明的有益效果:Beneficial effects of the present invention:

1.一级膜分离装置采用旋转式一级膜组件,利用气升式反应器内流体动能带动一级膜组件旋转,无需额外的能量输入,同时达到增加了膜与反应器液体接触面积、提高膜通透量的作用;同时利用流体冲洗膜表面,降低膜污染;一级膜组件的数量与安装角度可调节,适用不同气含率反应和不同分离性质产物,扩大了此反应器的应用范围;1. The first-stage membrane separation device adopts a rotating first-stage membrane module, which uses the fluid kinetic energy in the airlift reactor to drive the first-stage membrane module to rotate without additional energy input, and at the same time increases the liquid contact area between the membrane and the reactor and improves The role of membrane permeation; at the same time, use fluid to flush the membrane surface to reduce membrane fouling; the number and installation angle of the first-stage membrane modules can be adjusted, which is suitable for different gas holdup reactions and products with different separation properties, expanding the application range of this reactor ;

2.滤液收集器内可依据需要设置二级膜组件,进行二次膜分离,二级膜分离过程可以通过循环泵将滤液收集器中的一级滤液泵入釜体中循环,循环泵可根据生产工艺选择开启,扩大反应器适用范围,提高产物纯度;2. Secondary membrane modules can be set in the filtrate collector to perform secondary membrane separation. During the secondary membrane separation process, the primary filtrate in the filtrate collector can be pumped into the kettle body for circulation through the circulation pump. The circulation pump can be used according to The production process is selected and opened, the scope of application of the reactor is expanded, and the purity of the product is improved;

3.膜分离时可选择采用正压操作或者负压抽吸,在进行正压过滤时,可以调小反应釜顶部的通气口处阀门,从而增大釜内压力,实现膜分离;当釜内压力为常压或负压时则打开真空泵进行负压操作;还可以调节釜内压力来调节膜分离装置的工作与否,如当釜内为常压时,膜分离装置处于关闭状态,这样同时可以调节通气量以及膜通量来改变产物的移出量,从而可以根据反应速率进行相应的调整,可适用于对氧需求量不同的反应过程,操作更具灵活性;3. Positive pressure operation or negative pressure suction can be selected for membrane separation. When positive pressure filtration is performed, the valve at the vent on the top of the reactor can be turned down to increase the pressure in the reactor and realize membrane separation; when the pressure in the reactor When it is normal pressure or negative pressure, turn on the vacuum pump for negative pressure operation; you can also adjust the pressure in the kettle to adjust the operation of the membrane separation device. For example, when the pressure in the kettle is normal, the membrane separation device is closed, so that it can Adjust the ventilation rate and membrane flux to change the product removal rate, so that the corresponding adjustment can be made according to the reaction rate, which can be applied to the reaction process with different oxygen demand, and the operation is more flexible;

综上,旋转内循环气升式膜生物反应器打破传统膜反应器中膜组件区域化概念,集气升反应与膜分离于一体,将一级膜组件均匀分散安置在内循环气升式反应器的降液区,实现膜反应器与气升式反应器在生物发酵领域中的有效耦合,整个过程所需的能量大都由压缩气体提供,膜组件数量、角度、启用与否、膜通量都可以人工进行调节,以适应气升式反应器的不同操作,很好的实现了二者的耦合,具有能耗低、结构简单、操作灵活、反应条件温和等优点,为发酵生产多糖、寡糖等产物、底物分子量差别大的过程提供新的反应设备与工艺。In summary, the rotating internal circulation airlift membrane bioreactor breaks the concept of regionalization of membrane modules in traditional membrane reactors, integrates airlift reaction and membrane separation, and evenly disperses and arranges the first-stage membrane modules in the internal circulation airlift reaction The downcomer zone of the reactor realizes the effective coupling between the membrane reactor and the airlift reactor in the field of biological fermentation. Most of the energy required for the whole process is provided by compressed gas. Both can be manually adjusted to adapt to different operations of the airlift reactor, and the coupling between the two is well realized. It has the advantages of low energy consumption, simple structure, flexible operation, and mild reaction conditions. The process of sugar and other products and substrates with large molecular weight difference provides new reaction equipment and technology.

附图说明Description of drawings

图1是旋转内循环气升式膜生物反应器结构示意图;Fig. 1 is a schematic structural diagram of a rotating internal circulation airlift membrane bioreactor;

图2是一级膜分离装置俯视图;Fig. 2 is a top view of a primary membrane separation device;

图3是一级膜组件截面示意图;Fig. 3 is a schematic cross-sectional view of a first-stage membrane module;

图4是一级膜组件结构示意图;Fig. 4 is a structural schematic diagram of a first-stage membrane module;

图5是滤液收集器结构示意图;Fig. 5 is a schematic view of the structure of the filtrate collector;

图6是旋转内循环气升式膜生物反应器工艺系统图。Fig. 6 is a process system diagram of a rotating internal circulation airlift membrane bioreactor.

其中:1.进液口,2.导气管,3.导流筒,4.釜体,5.气体分布器,6.排液口,7.二级膜组件,8.滤液口,9.真空控制阀,10.循环调节阀,11.滤液收集器,12.滤液收集管,13.旋转架,14.密封轴承,15.一级膜组件,16.视窗,17.循环液入口,18.排气口,19.排气控制阀,20.通气阀,21.通气口,22.上旋盖,23.循环泵,24.罐体,25.滤液收集罐,26.真空泵,27.反冲控制阀,28.钢瓶,29.压缩空气机,30.气体流量计,31.循环液出口。Among them: 1. Liquid inlet, 2. Air duct, 3. Guide cylinder, 4. Kettle body, 5. Gas distributor, 6. Liquid outlet, 7. Secondary membrane module, 8. Filtrate port, 9. Vacuum control valve, 10. Circulation regulating valve, 11. Filtrate collector, 12. Filtrate collection pipe, 13. Swivel frame, 14. Sealed bearing, 15. Primary membrane module, 16. Window, 17. Circulating fluid inlet, 18 .Exhaust port, 19. Exhaust control valve, 20. Vent valve, 21. Vent port, 22. Screw cap, 23. Circulation pump, 24. Tank body, 25. Filtrate collection tank, 26. Vacuum pump, 27. Recoil control valve, 28. Steel cylinder, 29. Compressed air machine, 30. Gas flow meter, 31. Circulating fluid outlet.

具体实施方式detailed description

实施例一Embodiment one

如图1-图5所示,旋转内循环气升式膜生物反应器,包括反应釜主体、一级膜分离装置和滤液收集装置;反应釜主体包括导气管2、导流筒3、釜体4和气体分布器5,釜体4的顶部安装有压力表并设有进液口1、排气口18、通气口21,排气口18与排气控制阀19相连,通过调节排气量从而调节釜体4内的压力,通气口21与通气阀20相连,釜体4的外圆壁上设有视窗16、循环液入口17,釜体4的侧面安装有温度计、pH计、溶氧电极、液位探头并设有取样口,釜体4的底部设有排液口6,釜体4的内部安装有导流筒3,导流筒3的内部安装有导气管2,导气管2的上部与通气口21相连,下部与气体分布器5相连,气体分布器5为阶梯多环形,反应釜主体的高径比为3-10;一级膜分离装置包括旋转架13、密封轴承14和一级膜组件15,一级膜组件15为马蹄形截面的弯管式中空结构,可减轻整体重量,更易旋转,增大一级膜组件15与液体接触的比表面积,一级膜组件15可根据生产需要更换为超滤或微滤无机膜,可耐受灭菌操作,拦截菌体和未反应完全的多糖底物,一级膜组件15的数量可根据生产工艺选择,一级膜组件15的倾斜角度可人工调节为20-90度,一级膜组件15在进行液/固分离时进行正压过滤,跨膜压差为0.01~1MPa,或采用负压抽吸,跨膜压差为0.01~1MPa,在气升降液区的4根一级膜组件15轴向45度均布在导流筒3的外圆壁四周,一级膜组件15的两端分别可拆卸式固定在上下排布的两个旋转架13上,两个旋转架13的间距为导流筒3长度的0.1-1,旋转架13固定在密封轴承14上,密封轴承14套装在导流筒3上;滤液收集装置包括滤液收集器11和滤液收集管12,滤液收集器11包括密封轴承14、上旋盖22和罐体24,上旋盖22与罐体24连接,密封轴承14套装在上旋盖22上,滤液收集器11通过上旋盖22安装在釜体4的底部且位于气体分布器5的正下方,滤液收集器11与位于下部的旋转架13通过3根滤液收集管12呈120度间距相连,产生旋转力矩,使旋转顺利进行,同时也利于一级膜组件15被冲刷,防止污染,滤液收集器11内可选择安装二级膜组件7,可根据生产需要更换为超滤或微滤无机膜,可耐受灭菌操作,拦截菌体和未反应完全的多糖底物,罐体24的底部设有滤液口8,滤液口8与真空控制阀9相连,罐体24外圆壁的下部设有循环液出口31,循环液出口31与循环调节阀10相连。As shown in Figures 1 to 5, the rotating internal circulation airlift membrane bioreactor includes the main body of the reactor, the primary membrane separation device and the filtrate collection device; the main body of the reactor includes the air duct 2, the guide tube 3, and the reactor 4 and a gas distributor 5, a pressure gauge is installed on the top of the kettle body 4 and is provided with a liquid inlet 1, an exhaust port 18, and an air vent 21, and the exhaust port 18 is connected with the exhaust control valve 19, and the exhaust volume is adjusted Thereby regulating the pressure in the kettle body 4, the vent 21 is connected with the vent valve 20, the outer circular wall of the kettle body 4 is provided with a window 16, a circulating liquid inlet 17, and the side of the kettle body 4 is equipped with a thermometer, a pH meter, a dissolved oxygen Electrodes, liquid level probes and sampling ports are provided. The bottom of the kettle body 4 is provided with a liquid outlet 6. The inside of the kettle body 4 is equipped with a guide tube 3. The upper part of the reactor is connected to the vent 21, and the lower part is connected to the gas distributor 5. The gas distributor 5 is stepped and multi-ring, and the height-to-diameter ratio of the main body of the reactor is 3-10; the first-stage membrane separation device includes a rotating frame 13 and a sealed bearing 14 and the primary membrane assembly 15, the primary membrane assembly 15 is an elbow-type hollow structure with a horseshoe-shaped cross-section, which can reduce the overall weight, make it easier to rotate, and increase the specific surface area of the primary membrane assembly 15 in contact with the liquid. The primary membrane assembly 15 can Replace with ultrafiltration or microfiltration inorganic membranes according to production needs, which can withstand sterilization operations and intercept bacteria and unreacted polysaccharide substrates. The number of first-stage membrane modules 15 can be selected according to the production process. First-stage membrane modules 15 The inclination angle can be manually adjusted to 20-90 degrees. The first-stage membrane module 15 performs positive pressure filtration when liquid/solid separation is performed, and the transmembrane pressure difference is 0.01-1MPa, or negative pressure suction is used, and the transmembrane pressure difference is 0.01-1MPa, the four first-stage membrane modules 15 in the gas-lifting-liquid area are evenly distributed around the outer circular wall of the guide tube 3 at an axial angle of 45 degrees, and the two ends of the first-stage membrane modules 15 are detachably fixed on the upper and lower rows respectively. On the two rotating racks 13 of cloth, the distance between the two rotating racks 13 is 0.1-1 of the length of the guide tube 3, the rotating rack 13 is fixed on the sealed bearing 14, and the sealed bearing 14 is sleeved on the guide tube 3; the filtrate is collected The device includes a filtrate collector 11 and a filtrate collecting pipe 12, the filtrate collector 11 includes a sealed bearing 14, an upper screw cap 22 and a tank body 24, the upper screw cap 22 is connected with the tank body 24, and the sealed bearing 14 is set on the upper screw cap 22 , the filtrate collector 11 is installed on the bottom of the kettle body 4 through the upper screw cap 22 and is located directly below the gas distributor 5, and the filtrate collector 11 is connected to the lower rotating frame 13 through three filtrate collecting pipes 12 at an interval of 120 degrees , to generate a rotating torque, so that the rotation can be carried out smoothly, and it is also beneficial for the primary membrane module 15 to be washed away to prevent pollution. The secondary membrane module 7 can be installed in the filtrate collector 11, and can be replaced with ultrafiltration or microfiltration inorganic according to production needs. Membrane, which can withstand sterilization operations, intercept bacteria and unreacted polysaccharide substrates, the bottom of the tank body 24 is provided with a filtrate port 8, and the filtrate port 8 is connected to the vacuum control valve 9, and the lower part of the outer circular wall of the tank body 24 A circulating fluid outlet 31 is provided, and the circulating fluid outlet 31 is connected with the circulation regulating valve 10 .

如图1-图6所示,旋转内循环气升式膜生物反应器的工艺系统,旋转内循环气升式膜生物反应器的通气阀20依次与气体流量计30、钢瓶28、压缩空气机29相连;旋转内循环气升式膜生物反应器的真空控制阀9依次与滤液收集罐25、真空泵26相连;旋转内循环气升式膜生物反应器的循环调节阀10依次与循环泵23、旋转内循环气升式膜生物反应器的循环液入口17相连;气体流量计30依次与反冲控制阀27、真空控制阀9相连,可在生产结束后反冲洗一级膜组件15和二级膜组件7;应用于连续发酵产酶降解卡拉胶底物获得寡糖组分,通过原位灭菌后,通过进液口1向釜体4内加入料液,通过通气口21向其中鼓入压缩气体,气体经气体分布器5进入导流筒3,由于带有一定初速度的压缩气体的进入,在气升降液区与导流筒3之间产生密度差,在这两种的作用下,导流筒3内流体向上运动,气升降液区内液体向下运动,到达一级膜组件15时,可由真空控制阀9控制一级膜分离装置的开启;通过取样检测生物量、酶活测定等确定菌体产酶状况,达到产酶稳定时,12h左右,开启一级膜分离装置,其后进行连续发酵操作,一级膜分离装置始终开启;浓缩液经滤液收集管12进入滤液收集器11中,利用滤液收集器11中的中空纤维二级膜组件7,进行二级膜分离,使产物纯度和浓度同时提高;一级滤液需经滤液循环管路泵入釜体4,然后浓缩液收集到滤液收集罐25中;该实施方案还可用于琼胶寡糖、褐藻胶寡糖等产物、底物分子量差别大的发酵分离过程。As shown in Figures 1-6, the process system of the rotary internal circulation airlift membrane bioreactor, the vent valve 20 of the rotary internal circulation airlift membrane bioreactor is connected with the gas flow meter 30, the steel cylinder 28, the compressed air machine in sequence 29 connected; the vacuum control valve 9 of the rotary internal circulation airlift membrane bioreactor is connected with the filtrate collection tank 25 and the vacuum pump 26 successively; the circulation regulating valve 10 of the rotary internal circulation airlift membrane bioreactor is sequentially connected with the circulation pump 23, The circulating liquid inlet 17 of the rotary internal circulation airlift membrane bioreactor is connected; the gas flow meter 30 is connected with the recoil control valve 27 and the vacuum control valve 9 in sequence, and the first-stage membrane module 15 and the second-stage membrane module 15 can be backwashed after production is completed. Membrane module 7: applied to continuous fermentation to produce enzymes to degrade carrageenan substrate to obtain oligosaccharide components, after in-situ sterilization, feed liquid into the kettle body 4 through the liquid inlet 1, and blow into it through the vent 21 Compressed gas, the gas enters the guide tube 3 through the gas distributor 5, due to the entry of compressed gas with a certain initial velocity, there is a density difference between the gas lifting liquid area and the guide tube 3, under the action of these two , the fluid in the guide tube 3 moves upward, and the liquid in the air-lifting liquid area moves downward, and when it reaches the first-stage membrane module 15, the opening of the first-stage membrane separation device can be controlled by the vacuum control valve 9; the biomass and enzyme activity can be detected by sampling Determining the enzyme production status of the thalline by measuring, etc., when the enzyme production is stable, turn on the first-level membrane separation device for about 12 hours, and then carry out continuous fermentation operation, and the first-level membrane separation device is always open; the concentrated liquid enters the filtrate collection through the filtrate collection pipe 12 In the device 11, the hollow fiber secondary membrane module 7 in the filtrate collector 11 is used to perform secondary membrane separation, so that the product purity and concentration are improved at the same time; the primary filtrate needs to be pumped into the kettle body 4 through the filtrate circulation pipeline, and then concentrated The liquid is collected in the filtrate collection tank 25; this embodiment can also be used in the fermentation separation process of products such as agarose oligosaccharides and alginate oligosaccharides, and substrates with large molecular weight differences.

实施例二Embodiment two

如图1-图5所示,旋转内循环气升式膜生物反应器,包括反应釜主体、一级膜分离装置和滤液收集装置;反应釜主体包括导气管2、导流筒3、釜体4和气体分布器5,釜体4的顶部安装有压力表并设有进液口1、排气口18、通气口21,排气口18与排气控制阀19相连,通过调节排气量从而调节釜体4内的压力,通气口21与通气阀20相连,釜体4的外圆壁上设有视窗16、循环液入口17,釜体4的侧面安装有温度计、pH计、溶氧电极、液位探头并设有取样口,釜体4的底部设有排液口6,釜体4的内部安装有导流筒3,导流筒3的内部安装有导气管2,导气管2的上部与通气口21相连,下部与气体分布器5相连,气体分布器5为阶梯多环形,反应釜主体的高径比为3-10;一级膜分离装置包括旋转架13、密封轴承14和一级膜组件15,一级膜组件15为马蹄形截面的弯管式中空结构,可减轻整体重量,更易旋转,增大一级膜组件15与液体接触的比表面积,一级膜组件15可根据生产需要更换为超滤或微滤无机膜,可耐受灭菌操作,拦截菌体和未反应完全的多糖底物,一级膜组件15的数量可根据生产工艺选择,一级膜组件15的倾斜角度可人工调节为20-90度,一级膜组件15在进行液/固分离时进行正压过滤,跨膜压差为0.01~1MPa,或采用负压抽吸,跨膜压差为0.01~1MPa,在气升降液区的4根一级膜组件15轴向45度均布在导流筒3的外圆壁四周,一级膜组件15的两端分别可拆卸式固定在上下排布的两个旋转架13上,两个旋转架13的间距为导流筒3长度的0.1-1,旋转架13固定在密封轴承14上,密封轴承14套装在导流筒3上;滤液收集装置包括滤液收集器11和滤液收集管12,滤液收集器11包括密封轴承14、上旋盖22和罐体24,上旋盖22与罐体24连接,密封轴承14套装在上旋盖22上,滤液收集器11通过上旋盖22安装在釜体4的底部且位于气体分布器5的正下方,滤液收集器11与位于下部的旋转架13通过3根滤液收集管12呈120度间距相连,产生旋转力矩,使旋转顺利进行,同时也利于一级膜组件15被冲刷,防止污染,罐体24的底部设有滤液口8,滤液口8与真空控制阀9相连,罐体24外圆壁的下部设有循环液出口31,循环液出口31与循环调节阀10相连。As shown in Figures 1 to 5, the rotating internal circulation airlift membrane bioreactor includes the main body of the reactor, the primary membrane separation device and the filtrate collection device; the main body of the reactor includes the air duct 2, the guide tube 3, and the reactor 4 and a gas distributor 5, a pressure gauge is installed on the top of the kettle body 4 and is provided with a liquid inlet 1, an exhaust port 18, and an air vent 21, and the exhaust port 18 is connected with the exhaust control valve 19, and the exhaust volume is adjusted Thereby regulating the pressure in the kettle body 4, the vent 21 is connected with the vent valve 20, the outer circular wall of the kettle body 4 is provided with a window 16, a circulating liquid inlet 17, and the side of the kettle body 4 is equipped with a thermometer, a pH meter, a dissolved oxygen Electrodes, liquid level probes and sampling ports are provided. The bottom of the kettle body 4 is provided with a liquid outlet 6. The inside of the kettle body 4 is equipped with a guide tube 3. The upper part of the reactor is connected to the vent 21, and the lower part is connected to the gas distributor 5. The gas distributor 5 is stepped and multi-ring, and the height-to-diameter ratio of the main body of the reactor is 3-10; the first-stage membrane separation device includes a rotating frame 13 and a sealed bearing 14 and the primary membrane assembly 15, the primary membrane assembly 15 is an elbow-type hollow structure with a horseshoe-shaped cross-section, which can reduce the overall weight, make it easier to rotate, and increase the specific surface area of the primary membrane assembly 15 in contact with the liquid. The primary membrane assembly 15 can Replace with ultrafiltration or microfiltration inorganic membranes according to production needs, which can withstand sterilization operations and intercept bacteria and unreacted polysaccharide substrates. The number of first-stage membrane modules 15 can be selected according to the production process. First-stage membrane modules 15 The inclination angle can be manually adjusted to 20-90 degrees. The first-stage membrane module 15 performs positive pressure filtration when liquid/solid separation is performed, and the transmembrane pressure difference is 0.01-1MPa, or negative pressure suction is used, and the transmembrane pressure difference is 0.01-1MPa, the four first-stage membrane modules 15 in the gas-lifting-liquid area are evenly distributed around the outer circular wall of the guide tube 3 at an axial angle of 45 degrees, and the two ends of the first-stage membrane modules 15 are detachably fixed on the upper and lower rows respectively. On the two rotating racks 13 of cloth, the distance between the two rotating racks 13 is 0.1-1 of the length of the guide tube 3, the rotating rack 13 is fixed on the sealed bearing 14, and the sealed bearing 14 is sleeved on the guide tube 3; the filtrate is collected The device includes a filtrate collector 11 and a filtrate collecting pipe 12, the filtrate collector 11 includes a sealed bearing 14, an upper screw cap 22 and a tank body 24, the upper screw cap 22 is connected with the tank body 24, and the sealed bearing 14 is set on the upper screw cap 22 , the filtrate collector 11 is installed on the bottom of the kettle body 4 through the upper screw cap 22 and is located directly below the gas distributor 5, and the filtrate collector 11 is connected to the lower rotating frame 13 through three filtrate collecting pipes 12 at an interval of 120 degrees , to generate a rotating moment, so that the rotation can be carried out smoothly, and it is also beneficial to the first-stage membrane module 15 to be washed away to prevent pollution. The lower part of the wall is provided with a circulating fluid outlet 31 , and the circulating fluid outlet 31 is connected with the circulating regulating valve 10 .

如图1-图6所示,旋转内循环气升式膜生物反应器的工艺系统,旋转内循环气升式膜生物反应器的通气阀20依次与气体流量计30、钢瓶28、压缩空气机29相连;旋转内循环气升式膜生物反应器的真空控制阀9依次与滤液收集罐25、真空泵26相连;旋转内循环气升式膜生物反应器的循环调节阀10依次与循环泵23、旋转内循环气升式膜生物反应器的循环液入口17相连;气体流量计30依次与反冲控制阀27、真空控制阀9相连,可在生产结束后反冲洗一级膜组件15;应用于深层发酵法生产灵芝多糖的过程,通过进液口1向釜体4内加入料液,通过通气口21向其中鼓入压缩气体,气体经气体分布器5进入导流筒3,导流筒3内流体向上运动,气升降液区内液体向下运动,到达一级膜组件15时,打开真空泵26,调节跨膜压差,进行负压抽吸,使膜通量与补料速率一致,以保持罐内液面平衡;浓缩液经滤液收集管12进入滤液收集器11中,然后浓缩液收集到滤液收集罐25中,由于菌体无法通过微滤膜,菌体滞留在釜体4内,使得罐内含有较高的细胞浓度;该实施方案还可用于香菇多糖、云芝多糖、猴头菇多糖等一级膜过滤。As shown in Figures 1-6, the process system of the rotary internal circulation airlift membrane bioreactor, the vent valve 20 of the rotary internal circulation airlift membrane bioreactor is connected with the gas flow meter 30, the steel cylinder 28, the compressed air machine in sequence 29 connected; the vacuum control valve 9 of the rotary internal circulation airlift membrane bioreactor is connected with the filtrate collection tank 25 and the vacuum pump 26 successively; the circulation regulating valve 10 of the rotary internal circulation airlift membrane bioreactor is sequentially connected with the circulation pump 23, The circulating liquid inlet 17 of the rotary internal circulation air-lift membrane bioreactor is connected; the gas flow meter 30 is connected with the recoil control valve 27 and the vacuum control valve 9 in turn, and the primary membrane module 15 can be backwashed after the production is completed; In the process of producing Ganoderma lucidum polysaccharide by submerged fermentation method, feed liquid is added into the kettle body 4 through the liquid inlet 1, and compressed gas is blown into it through the vent 21, and the gas enters the guide tube 3 through the gas distributor 5, and the guide tube 3 The internal fluid moves upwards, and the liquid in the gas-lifting liquid area moves downwards. When it reaches the first-stage membrane module 15, the vacuum pump 26 is turned on to adjust the transmembrane pressure difference and carry out negative pressure suction to make the membrane flux consistent with the feeding rate. Keep the liquid level balance in the tank; the concentrated solution enters the filtrate collector 11 through the filtrate collection pipe 12, and then the concentrated solution is collected in the filtrate collection tank 25, because the thalline cannot pass through the microfiltration membrane, the thalline remains in the still body 4, The tank contains a higher cell concentration; this embodiment can also be used for primary membrane filtration such as lentinan, versicolor polysaccharide, and Hericium erinaceus polysaccharide.

以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明披露的技术范围内,根据本发明的技术方案及其发明构思加以等同替换或改变,都应涵盖在本发明的保护范围之内。The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, and any person familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solution of the present invention Any equivalent replacement or change of the inventive concepts thereof shall fall within the protection scope of the present invention.

Claims (10)

1. rotate internal circulation gas-lift type membrane bioreactor, it is characterised in that: include reactor main body, one-level film Segregation apparatus and Filtrate collection device;Reactor main body includes airway (2), guide shell (3), kettle (4) With gas distributor (5), the top of kettle (4) is provided with Pressure gauge and is provided with inlet (1), aerofluxus Mouth (18), blow vent (21), air vent (18) is connected with gas exhausting valve (19), blow vent (21) Being connected with breather valve (20), the cylindrical wall of kettle (4) is provided with form (16), circulation fluid entrance (17), The bottom of kettle (4) is provided with leakage fluid dram (6), and the internally installed of kettle (4) has guide shell (3), water conservancy diversion Cylinder the internally installed of (3) has airway (2), and the top of airway (2) is connected with blow vent (21), Bottom is connected with gas distributor (5), and gas distributor (5) is that how annular ladder is;
One-level membrane separation device includes swivel mount (13), seals bearing (14) and one-level membrane module (15), One-level membrane module (15) is the bent-tube boiler hollow structure of horseshoe shaped section, some described one-level membrane modules (15) being distributed on the cylindrical wall surrounding of guide shell (3), the two ends of one-level membrane module (15) are the most removable The formula of unloading is fixed on two swivel mounts (13) arranged up and down, and swivel mount (13) is fixed on sealing bearing (14) On, seal bearing (14) and be sleeved on guide shell (3);
Filtrate collection device includes filtrate collection (11) and percolate collection tubes (12), filtrate collection (11) Including sealing bearing (14), upper spiral cover (22) and tank body (24), upper spiral cover (22) and tank body (24) Connecting, seal bearing (14) and be sleeved on spiral cover (22), filtrate collection (11) is by upper spiral cover (22) it is arranged on the bottom of kettle (4) and is positioned at the underface of gas distributor (5), filtrate collection (11) it is connected by percolate collection tubes (12) with the swivel mount (13) being positioned at bottom, tank body (24) Bottom is provided with filtrate port (8), and filtrate port (8) is connected with vacuum control valve (9), tank body (24) cylindrical The bottom of wall is provided with circulation fluid outlet (31), and circulation fluid outlet (31) is connected with circulating regulation valve (10).
Rotation internal circulation gas-lift type membrane bioreactor the most according to claim 1, it is characterised in that: institute In the filtrate collection (11) stated, second membrane module (7) is installed.
Rotation internal circulation gas-lift type membrane bioreactor the most according to claim 1, it is characterised in that: institute The 0.1-1 that spacing is guide shell (3) length of two swivel mounts (13) stated.
Rotation internal circulation gas-lift type membrane bioreactor the most according to claim 1, it is characterised in that: institute The ratio of height to diameter stating reactor main body is 3-10.
Rotation internal circulation gas-lift type membrane bioreactor the most according to claim 1, it is characterised in that: institute The angle of inclination stating one-level membrane module (15) is 20-90 degree.
Rotation internal circulation gas-lift type membrane bioreactor the most according to claim 1, it is characterised in that: institute The side stating kettle (4) is provided with thermometer, pH meter, dissolved oxygen electrode, liquid-level probe and is provided with sample tap.
Rotation internal circulation gas-lift type membrane bioreactor the most according to claim 1, it is characterised in that: institute The filtrate collection (11) stated and the swivel mount (13) being positioned at bottom pass through 3 percolate collection tubes (12) It is connected in 120 degree of spacing.
8. applying the system rotating internal circulation gas-lift type membrane bioreactor described in claim 1, it is special Levy and be: the breather valve (20) of described rotation internal circulation gas-lift type membrane bioreactor successively with gas flow Meter (30), steel cylinder (28), air compressor (29) are connected;Rotate internal circulation gas-lift type membrane biological reaction The vacuum control valve (9) of device is connected with filtrate collection tank (25), vacuum pump (26) successively;Follow in rotation The circulating regulation valve (10) of ring gas-lifting type membrane bioreactor circulates with in circulating pump (23), rotation successively The circulation fluid entrance (17) of gas-lifting type membrane bioreactor is connected.
System the most according to claim 8, it is characterised in that: described rotation internal circulation gas-lift type film is biological In the filtrate collection (11) of reactor, second membrane module (7) is installed.
System the most according to claim 8, it is characterised in that: described gas flowmeter (30) is successively It is connected with recoil control valve (27), vacuum control valve (9).
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