CN117054673A - Full-automatic luminous immunoassay detector - Google Patents
Full-automatic luminous immunoassay detector Download PDFInfo
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- CN117054673A CN117054673A CN202310889485.3A CN202310889485A CN117054673A CN 117054673 A CN117054673 A CN 117054673A CN 202310889485 A CN202310889485 A CN 202310889485A CN 117054673 A CN117054673 A CN 117054673A
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- 238000003018 immunoassay Methods 0.000 title claims abstract description 30
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 493
- 239000007788 liquid Substances 0.000 claims abstract description 167
- 238000005406 washing Methods 0.000 claims abstract description 118
- 238000001514 detection method Methods 0.000 claims abstract description 65
- 238000012545 processing Methods 0.000 claims abstract description 60
- 239000000758 substrate Substances 0.000 claims abstract description 40
- 230000036046 immunoreaction Effects 0.000 claims abstract description 32
- 238000005070 sampling Methods 0.000 claims abstract description 27
- 238000007789 sealing Methods 0.000 claims description 65
- 239000002699 waste material Substances 0.000 claims description 65
- 239000003550 marker Substances 0.000 claims description 34
- 239000000243 solution Substances 0.000 claims description 30
- 238000004020 luminiscence type Methods 0.000 claims description 27
- 238000012546 transfer Methods 0.000 claims description 26
- 238000000605 extraction Methods 0.000 claims description 21
- 239000012528 membrane Substances 0.000 claims description 17
- 238000005086 pumping Methods 0.000 claims description 17
- 238000003825 pressing Methods 0.000 claims description 16
- 238000012360 testing method Methods 0.000 claims description 16
- 238000002347 injection Methods 0.000 claims description 14
- 239000007924 injection Substances 0.000 claims description 14
- 238000003860 storage Methods 0.000 claims description 14
- 230000007246 mechanism Effects 0.000 claims description 13
- 238000005119 centrifugation Methods 0.000 claims description 11
- 230000008105 immune reaction Effects 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 9
- 230000006835 compression Effects 0.000 claims description 7
- 238000007906 compression Methods 0.000 claims description 7
- 238000001746 injection moulding Methods 0.000 claims description 5
- 239000006210 lotion Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 239000007790 solid phase Substances 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 3
- 230000003044 adaptive effect Effects 0.000 claims description 3
- 238000010586 diagram Methods 0.000 description 11
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 230000006872 improvement Effects 0.000 description 10
- 210000002381 plasma Anatomy 0.000 description 3
- 238000012123 point-of-care testing Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000036470 plasma concentration Effects 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 206010008479 Chest Pain Diseases 0.000 description 1
- 102000029749 Microtubule Human genes 0.000 description 1
- 108091022875 Microtubule Proteins 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 210000004688 microtubule Anatomy 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
Abstract
A full-automatic luminous immunoassay detector comprises a shell, a sample processing device, an automatic sampling device, a dry integrated reagent access device, an integrated reagent immunoreaction and washing device, a luminous substrate liquid filling reaction and photon detection counting and data processing device and a control center module; the sample processing device, the automatic sample feeding device, the dry integrated reagent access device, the integrated reagent immunoreaction and washing device, the luminescent substrate liquid filling reaction and photon detection counting and data processing device are all arranged in the inner cavity and controlled by the control center module. The full-automatic luminous immunoassay detector has high detection efficiency.
Description
Technical Field
The invention relates to a full-automatic luminous immunoassay detector, and belongs to the technical field of luminous immunoassay.
Background
To date, luminescence analysis techniques have been widely used in the field of clinical testing because of their high sensitivity, wide linear analysis range, good stability, and automation. The automatic luminous immunoassay detection system takes a hospital center laboratory and a third party detection inspection mechanism as using objects, develops to a high-flux assembly line, and develops to the demands of middle and small medical institution clients.
In the field of clinical medicine testing, it is often desirable to report plasma concentrations on-board whole blood samples. The detection of whole blood samples is often performed using an automated luminescent immunoassay detection system. When the mainstream automatic luminous immunoassay detection system adopts a magnetic particle solid phase, the reaction efficiency is reduced due to the blocking of blood cells when the whole blood sample is directly used for detection reaction, and the accurate plasma concentration is obtained, so that most of use scenes are that the collected blood sample tube is manually transferred and concentrated at a place with centrifugal sample processing equipment, and a great deal of time is consumed in the aspects of sample transfer, classification, centrifugal processing and the like because the whole blood sample is fully centrifuged outside a whole blood sample machine to obtain the plasma for detection.
Because of this, although the degree of automation of clinical test platforms is greatly improved at present, the test turnover (TAT) of the overall test result report is limited to patient benefits, even if an automatic luminescence immunoassay test system is used, except for chest pain centers and few items of emergency treatment, for other immunoassay item tests. Most patients still face reports of results from immunodetection programs obtained on alternate days or shifts. Patients still need to endure the pain and take time to get the physician to diagnose and enter treatment. This clearly makes a gap with the patient's expectations. It is difficult to meet the requirements of point-of-care testing (Point of Care Test, POCT).
Disclosure of Invention
The invention provides a full-automatic luminous immunoassay detector which can effectively solve the problems, and the invention is realized in the following way:
a full-automatic luminous immunoassay detector comprises a shell, a sample processing device (10), an automatic sampling device (20), a dry integrated reagent access device (30), an integrated reagent immunoreaction and washing device (40), a luminous substrate liquid filling reaction and photon detection counting and data processing device (50) and a control center module;
an inner cavity is arranged in the shell; the sample processing device (10), the automatic sample feeding device (20), the dry integrated reagent access device (30), the integrated reagent immune reaction and washing device (40), the luminescent substrate liquid filling reaction and photon detection counting and data processing device (50) are all arranged in the inner cavity and controlled by the control center module; a plurality of dry integrated reagents are stored on the dry integrated reagent storing and taking device (30), and an integrated reagent sealing film (32222) is covered on the dry integrated reagents;
the sample processing device (10) comprises a centrifugal device (11), and is controlled by the control center module to automatically perform centrifugal processing on a sample;
the automatic sample injection device (20) is controlled by the control center module to automatically add the centrifugally processed sample into the dry integrated reagent of the integrated reagent immunoreaction and washing device (40);
The dry integrated reagent storing and taking device (30) stores a plurality of dry integrated reagents, and is controlled by the control center module to automatically grab the dry integrated reagents into the integrated reagent immune reaction and washing device (40) and tear off an integrated reagent sealing film (32222);
the integrated reagent immunoreaction and wash apparatus (40) comprises an immunoreaction element and a wash element; the immunoreactive component comprises a dry marker solution filling device (42) for carrying out immunoreactions in the detection of the sample and the dry integrated reagent; the washing element is controlled by the control center module to automatically wash the immunoreaction element;
the luminous substrate liquid filling reaction and photon detection counting and data processing device (50) comprises a luminous substrate liquid filling element and a photon detection counting and data processing element; the luminous substrate liquid filling element is controlled by the control center module to automatically fill luminous substrate liquid into the immunoreaction element for luminous reaction; the photon detection counting and data processing element is controlled by the control center module to automatically detect the luminescence reaction and report the detection result of the sample.
As a further improvement, the sample processing device (10) comprises a centrifugal device (11), a cover opening clamping device (12), a sample tube clamp (13), a sample tube transferring device (14) and a sample feeding disc (15);
The centrifugal device (11) comprises a centrifugal cavity (111), a centrifugal rotor (112), a first sample tube position (113), a rotor core (115) and a first driving motor (116); the centrifugal rotor (112) is positioned in the centrifugal cavity (111), the first sample tube position (113) is positioned on the centrifugal rotor (112), the rotor core (115) is positioned on the central shaft of the centrifugal rotor (112), the rotor core (115) is electrically connected with the first driving motor (116), and the first driving motor (116) provides driving force for centrifugation; the centrifugal cavity (111) limits the centrifugal process to the space where the centrifugal rotor (112) is located, the first sample tube position (113) is formed by arranging holes on the upper surface and the lower surface of the centrifugal rotor (112), and the sample tube is limited in the upper hole and the lower hole and is used for fixing the sample tube; the rotor core (115) is a dumbbell-shaped middle structure hollowed out in the middle of the centrifugal rotor (112) so as to expose the sample tube body. An upper end opening of the centrifugal cavity (111); the side wall of the centrifugal cavity (111) is provided with a sample tube clamping hollow (114); a support rod is arranged beside the centrifugal device (11).
The cover opening clamping and lifting device (12) is arranged above the upper end opening of the centrifugal cavity (111); the cover opening clamp handle (12) comprises an automatic telescopic rod, the lower end of the automatic telescopic rod is provided with an automatic clamp for clamping a tube cover of the sample tube, and the upper end of the automatic telescopic rod is fixedly connected to the top of the inner cavity of the shell;
A supporting rod is arranged beside the centrifugal device (11);
one end of the sample tube clamp (13) is arranged on the supporting rod, and the other end of the sample tube clamp is provided with an automatic clamp for clamping the tube wall of the sample tube and extends into a sample tube clamp hollow-out hole (114) on the side wall of the centrifugal cavity (111);
one end of the sample tube transferring device (14) is connected to the supporting rod and can automatically rotate and automatically slide up and down along the supporting rod, and the other end of the sample tube transferring device is provided with an automatic clamp for clamping a sample tube;
the sample injection disc (15) comprises a disc wall body (151), a chassis turntable (154), a radio frequency scanning identifier and a second driving motor (155); the tray wall body (151) is arranged on the tray turntable (154), a second sample tube position (152) is arranged on the tray wall body (151), a sample tube identification hollow-out hole (153) is formed in the side wall of the tray wall body (151), the radio frequency scanning identifier scans and identifies sample tube information through the sample tube identification hollow-out hole (153), and a second driving motor (155) provides power for rotation of the sample feeding tray (15).
As a further improvement, the automatic sampling device (20) comprises a suction head (22) for sampling, a suction head box (21) for storing the suction head, a driving sampling sampler (23) and a slideway 24 for driving the sampling sampler (23) to move at different stations; the lower end of the driving sampling injector (23) is provided with a suction nozzle for inserting the suction nozzle (22); the upper end of the driving sampling sampler (23) is connected with a slide bar which is connected on the slide way (24) in a sliding way.
As a further improvement, the dry integrated reagent access device (30) comprises an integrated reagent storage structure (32), an integrated reagent seat push-pull structure (33) and a mechanical arm (34) for grasping and placing integrated reagent and tearing a sealing film; the integrated reagent storage structure is used for storing integrated reagents, and the integrated reagent seat push-pull structure (33) and the grabbing and placing integrated reagents and the tearing and sealing film mechanical arm (34) are matched with each other to grab and place the integrated reagents (3222) on the integrated reagent storage structure (32) into the integrated reagent immunoreaction and washing device (40).
As a further improvement, the integrated reagent storage structure (32) comprises an integrated reagent tray supporting cylinder (312), a plurality of layers of equidistance integrated reagent tray trays (321) are arranged on the outer wall of the integrated reagent tray supporting cylinder (312), a plurality of integrated reagent tray slide ways (325) are arranged on the integrated reagent tray trays (321), integrated reagent trays (322) are arranged in the integrated reagent tray slide ways (325), a plurality of integrated reagent plastic bodies (32223) are arranged in the integrated reagent seat (3223), the integrated reagent seat (3223) is of an injection molding multiposition hollow structure, the integrated reagent plastic bodies (32223) comprise integrated reagent micro-reaction tubes (32211) and integrated reagent marker tubes (32213), the integrated reagent micro-reaction tubes (32211) and the integrated reagent marker tubes (32213) are in one-to-one correspondence injection molding, the bottom micro-structures (32211) are micro-structures of various rotating bodies, pyramid shapes or platforms and the like protruding upwards from cavities, the integrated reagent micro-reaction tubes (32211) are solid phase immunoreaction reagents, the integrated reagent marker tubes (3223) are in the form a plurality of integrated reagent card structures, the integrated reagent plastic bodies (32223) are formed into a plurality of integrated reagent connecting pieces (35) in a whole plastic connecting piece (35), and the integrated reagent connecting sheet (32214) is disconnected at a certain stage of the preparation of the integrated reagent (3222) to form the independent integrated reagent (3222); an integrated reagent separation slit (32221) is formed by completely breaking a certain step of the preparation of the integrated reagent (3222) between adjacent integrated reagent plastic bodies (32223), and an integrated reagent micro-reaction tube (32211) and an integrated reagent marker tube (32213) are covered by an integrated reagent sealing film (32222);
A sector-shaped vacancy area which is equal to the sector where the integrated reagent disk (322) is positioned is reserved on each layer of integrated reagent disk tray (321) and is a circular disk tray sector-shaped vacancy (324);
the bottom of integrated reagent dish tray support drum (312) sets up liftable motor (311), lift motor (311) contain electromagnetic materials swinging boom, integrated reagent dish tray support drum (312) are the central pivot position of each layer integrated reagent dish tray (321), rotatory inner ring on integrated reagent dish tray (321) is annular rotatory slider structure (323), the tray support (326) of integrated reagent dish tray (321) is the rotatory channel structure under the cooperation of rotatory slider structure (323) of support on the outer wall of integrated reagent dish tray support drum (312), one department of rotatory slider structure (323) is equipped with permanent magnetic body, electromagnetic materials swinging boom matching with liftable motor (311), both inhale after the circular telegram, drive integrated reagent dish tray (321) are rotatory, each layer integrated reagent dish tray (321) is rotatory independently under the electromagnetic materials matching effect of the lifting motor (311) of rotatory slider (323), tray support (326), the target integrated reagent dish is found.
As a further improvement, the integrated reagent seat push-pull structure (33) comprises an integrated reagent seat push-pull sliding slideway (331), a scissors structure (334), an integrated reagent disc grabbing and pulling mechanism (335), an integrated reagent disc reciprocating sliding motor (332) and a scissors structure driving motor (333); the scissors structure (334) is formed by crossing two struts, the crossing is connected by a scissors fork shaft (336), a sliding rod is arranged between the bottom ends of the two struts, a scissors structure driving motor (333) is arranged on the sliding rod, one end of the bottom of one of the two struts is driven by the scissors structure driving motor (333) to slide on the sliding rod, and then the scissors structure (334) is driven to open and close, so that the integrated reagent tray grabbing and pulling mechanism (335) is lifted, and the integrated reagent tray reciprocating sliding motor (332) drives the supporting frame to slide on the integrated reagent tray push-pull sliding slideway 331, so that the whole integrated reagent tray push-pull structure (33) is driven to slide.
As a further improvement, the mechanical arm (34) for grabbing and placing the integrated reagent and tearing the sealing film comprises a cantilever beam (341), a horizontal travel motor (342), a horizontal beam (343), a horizontal movement motor (344), a vertical travel motor (345), a vertical beam (3461), a cross beam (3462), an integrated reagent lifting and inserting motor (3463), an integrated reagent lifting and inserting vertical rod (3464), an integrated reagent lifting rod (3465), an integrated reagent pressing rod (3471), an integrated reagent pressing rod vertical (3472), an integrated reagent sealing film clamping and lifting motor (3473), an integrated reagent sealing film clamping and lifting rod (3474) and an integrated reagent sealing film clamping piece (3475); the cantilever beam (341) is fixed on the side wall or the top structure of the shell, a slide way is arranged on the cantilever beam (341), the horizontal beam (343) extends outwards from the axis of the integrated reagent tray (321) to form a parallel structure, one end of the horizontal beam (343) is connected to the cantilever beam (341), a traverse motor (342) is arranged at the joint of the horizontal beam (343) and the cantilever beam (341), the traverse motor (342) drives the integrated reagent to be placed and the film tearing mechanical arm 34 to be pulled out into the integrated reagent tray (321), the other end of the horizontal beam (343) is connected to a vertical beam 3461, a horizontal moving motor (344) is arranged at the joint of the horizontal beam (343) and the vertical beam (3461), the horizontal moving motor (344) drives the horizontal beam (343) to be close to or far away from the integrated reagent tray (321), a vertical moving motor (345) is arranged below the horizontal moving motor (344) on the vertical beam (3461), and the vertical moving motor (345) drives the integrated reagent to be placed and the film tearing mechanical arm 34 to move up and down along the vertical beam (3461); the cross beam (3462) is a transverse extension structure in the middle of the vertical beam (3461), and an integrated reagent lifting and inserting motor (3463) is arranged on the cross beam; one end of an integrated reagent lifting and inserting vertical rod (3464) is connected to an integrated reagent lifting and inserting motor (3463), and the other end is connected to an integrated reagent lifting rod (3465); an integrated reagent lifting and inserting motor (3463) drives an integrated reagent lifting and inserting vertical rod (3464) to be close to or separated from an integrated reagent plastic body (32223); the integrated reagent compression bar (3471) is of a transverse structure perpendicular to the vertical beam (3461) and is positioned at the bottom of the vertical beam (3461); the integrated reagent pressing rod is erected (3472) in an upward extending structure at one end of the integrated reagent pressing rod (3471), one end of the integrated reagent sealing film clamping lifting rod (3474) is connected to the integrated reagent pressing rod erected (3472), the integrated reagent sealing film clamping lifting motor (3473) is positioned at the joint of the integrated reagent sealing film clamping lifting rod (3474) and the integrated reagent pressing rod erected (3472), and the integrated reagent sealing film clamping lifting motor (3473) drives the integrated reagent sealing film clamping lifting rod (3474) to move up and down so as to clamp and release the integrated reagent sealing film (32222) and assist in tearing the film; the integrated reagent sealing membrane clamping lifting rod (3474) is an upright structure connected with the integrated reagent sealing membrane clamping lifting motor (3473), and extends transversely below the integrated reagent sealing membrane clamping lifting rod to form an integrated reagent sealing membrane clamping piece (3475).
As a further improvement, the integrated reagent immune reaction and washing device (40) comprises a reaction disc (41) and a driving motor thereof, a marker solution filling device (42), a hair washing device (43), a washing liquid filling device (44), a waste liquid pumping device (45) and a post-washing integrated reagent tube transfer arm (46); the reaction disk (41) is arranged at the top end of the dry integrated reagent access device (30); the reaction tray (41) is provided with a plurality of reaction stations for the reaction of the integrated reagent (3222), a marker solution filling device (42) is arranged above the next station which is close to the loading station of the integrated reagent (3222), and more than 2 washing stations for washing the washing heads (43) are arranged; a sample injection station is also arranged on the reaction disc (41) for the automatic sample injection device to feed samples;
the marker solution filling device (42) comprises a marker solution container, a motor for driving the marker solution, a liquid flow control valve and a liquid guide tube;
the lotion-filling device (44) is a container containing lotion, a motor for driving the lotion, a flow control valve and a catheter; the waste liquid pumping and draining device (45) comprises a waste liquid container, a motor for driving waste liquid to be pumped, a liquid flow control valve and a liquid guide pipe;
the post-wash integrated reagent tube transfer arm (46) includes a cross arm and an automatic gripping device attached to the cross arm.
As a further improvement, the hair washing (43) comprises a hair washing cavity (435), a washing liquid filling hair washing inlet (431), a washing liquid filling micro-reaction tube outlet (432), a waste liquid extraction micro-reaction tube suction head (433) and a waste liquid extraction hair washing outlet (434); a wash liquor filling and washing head inlet (431) is communicated with a wash liquor filling device (44), and the wash liquor filling and washing head inlet (431) is communicated with a wash liquor filling micro-reaction tube outlet (432); the waste liquid extraction micro-reaction tube suction head (433) is communicated with a waste liquid extraction and washing outlet (434), and the waste liquid extraction and washing outlet (434) is communicated with a waste liquid extraction and discharge device (45); a wash liquid filling wash head inlet (431) for quantitatively introducing a wash liquid, and a wash liquid filling micro-reaction tube outlet (432) for guiding the wash liquid into the integrated reagent micro-reaction tube (32211) in a directional manner; the waste liquid sucking head (433) of the waste liquid sucking micro-reaction tube is a structure for sucking waste liquid, which is adaptive to the integrated reagent micro-reaction tube (32211), and the waste liquid sucking head for sucking and discharging is a horn-shaped structure with the bottom caliber larger than the tube diameter of the sucking tube; the hair washing cavity (435) is a cavity channel separated by the internal washing liquid and the waste liquid.
As a further improvement, the luminescent substrate liquid filling reaction and photon detection counting and data processing device (50) comprises a luminescent reaction disc (51), a luminescent substrate liquid filling device (52), a photon detection counting and data processing device (53) and a test integrated reagent tube transfer arm (54);
A plurality of stations are arranged on the luminous reaction disk (51) for luminous reaction and photon detection counting; the luminous substrate liquid filling device (52) comprises a luminous liquid container, a motor for driving luminous liquid, a liquid flow control valve and a liquid guide tube; the post-test integrated reagent tube transfer arm (54) includes a cross arm and an automatic gripping device connected to the cross arm.
The beneficial effects of the invention are as follows:
the sample processing device of the full-automatic luminous immunoassay detector comprises a centrifugal device, a whole blood sample tube is adopted to directly enter a machine for centrifugal treatment, and blood plasma (serum) obtained by centrifugation is used for detection, so that the accuracy and reliability of a detection result are ensured, the steps of sample tube transmission and centralized centrifugal treatment in conventional detection are omitted, the time consumption is reduced, the detection items required by patients can be rapidly reported, and the detection efficiency is greatly improved.
The dry integrated reagent storage and taking device of the full-automatic luminous immunoassay detector stores various integrated reagents, realizes automatic grabbing through the integrated reagent seat push-pull structure and the grabbing and placing integrated reagents and the film sealing mechanical arm tearing, omits the process of adding multiple components of the detection reagents during detection, and saves the running time of the detector.
The full-automatic luminous immunoassay detector of the invention realizes continuous waste liquid pumping and draining during washing, and pulse-type washing liquid injection can form certain pressure and liquid flow washing for the side wall and the bottom of the micro-reaction tube, thereby realizing rapid and thorough washing, ensuring the detection result, shortening the washing time and improving the running speed of the whole machine.
The full-automatic luminous immunoassay detector can realize automatic centrifugation, automatic sampling and sample feeding, automatic taking and placing of integrated reagents, automatic washing, automatic luminous reaction and inspection result output, realize full automation, convenient operation, simultaneously can load a plurality of integrated reagents, realize simultaneous detection of a plurality of items and have high detection flux.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is an overall schematic diagram of a full-automatic luminescence immunoassay detector according to an embodiment of the present invention.
FIG. 2 is an overall schematic diagram of a sample processing device 10 according to an embodiment of the present invention.
Fig. 3 is a schematic view of a centrifuge device of the sample processing device 10 according to an embodiment of the present invention.
Fig. 4 is a schematic diagram of a sample tray of the sample processing device 10 according to an embodiment of the present invention.
Fig. 5 is a schematic diagram of an automatic sample injection device according to an embodiment of the invention.
FIG. 6 is a schematic diagram of a dry integrated reagent access device according to an embodiment of the present invention.
FIG. 7 is a schematic diagram of an integrated reagent storage structure of a dry integrated reagent access device according to an embodiment of the present invention.
FIG. 8 is a schematic diagram of an integrated reagent tray of a dry integrated reagent access device according to an embodiment of the present invention.
FIG. 9 is a schematic diagram of an integrated reagent holder push-pull structure of a dry integrated reagent access device according to an embodiment of the present invention.
FIG. 10 is a schematic view of a gripper and gripper integrated with a reagent and a film tearing mechanical arm according to an embodiment of the present invention.
FIG. 11 is a schematic diagram of an integrated reagent immune reaction device and washing device according to an embodiment of the present invention.
FIG. 12 is a schematic diagram of a shampoo of an integrated reagent immune reaction device and washing device according to an embodiment of the present invention.
FIG. 13 is a schematic diagram of a luminescent substrate liquid filling reaction and photon detection counting and data processing device according to an embodiment of the invention.
Reference numerals:
10 sample processing device; 11 a centrifugal device; 111 centrifugal cavity; 112 centrifuging the rotor; 113 a first sample tube position; 114 sample tube clamp hollowed-out; 115 rotor cores; 116 a first drive motor; 12, opening the cover and clamping; 13 sample tube clamps; 14 sample tube transfer means; 15 sample feeding discs; 151 disc wall body, 152 second sample tube position; 153 sample tube identification hollowed-out; 154 turntables; 155 a second drive motor;
20 an automatic sample feeding device; 21 a tip cassette; 22 disposable tips; 23 driving a sampling injector; 24 slides;
30 a dry integrated reagent access device; 311 a liftable motor; 312 an integrated reagent tray support cylinder; 321 integrated reagent tray; 322 an integrated reagent disk; 32211 are integrated with reagent micro-reaction tubes; 32212 a bottom microstructure; 32213 integrated reagent marker tube; 32214 integrated reagent tab; 3222 integrating reagents; 32221 are integrated with a reagent separation slit; 32222 an integrated reagent sealing membrane; 32223 an integrated reagent plastic body; 3223 an integrated reagent holder; 323 rotating the slider; 324 circular tray sector segment; 325 integrated reagent tray slide; 326 a tray support for the integrated reagent tray; 331 integrated reagent seat push-pull sliding slideway; 332 integrated reagent disk shuttle motor; 333 scissors configuration drive motor; 334 scissors configuration; 335 an integrated reagent disk gripping mechanism; 34, grabbing and placing the integrated reagent and tearing the sealing film mechanical arm; 341 a cantilever beam; 342 traverse motor; 343 horizontal beams; 344 a horizontal movement motor; 345 vertical travel motor; 3461 vertical beams; 3462 cross beams; 3463 Integrated reagent lift and insert Motor; 3464 lifting and inserting a vertical rod by an integrated reagent; 3465 integrated reagent lifting lever; 3471 Integrated reagent compression bar; 3472 the integrated reagent compression bar is erected; 3473 Integrated reagent seal Membrane clamping Motor; 3474 Integrated reagent seal Membrane clip lifting rod; 3475 Integrated reagent sealing Membrane clamping sheet; 351 barrel cylinder wall; 352 barrel cylinder wall gaps;
40 integrating a reagent immune reaction and washing device; 41 a reaction plate and a driving motor thereof; 42; a marker solution filling device; 43 washing hair; 431 washing liquid is filled into the hair washing inlet; 432 washing liquid is filled in the outlet of the micro-reaction tube; 433 waste liquid is pumped out of the micro-reaction tube suction head; 434 waste liquid is pumped out of the hair washing outlet; 435 a hair washing cavity; 44 a lotion filling device; 45 waste liquid pumping and draining device; 46, integrating a reagent tube transfer arm after washing;
50 luminous substrate liquid filling reaction and photon detection counting and data processing device, 51 luminous reaction disk; 52 luminescent substrate liquid filling device; 53 photon detection counting and data processing device; 54 test integrated reagent tube transfer arm.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
The automatic control involved in the embodiment of the invention is a conventional automatic control means in the field.
As shown in fig. 1, an embodiment of the present invention provides a full-automatic luminescence immunoassay detector, which comprises a housing, a sample processing device 10, an automatic sample injection device 20, a dry integrated reagent access device 30, an integrated reagent immunoreaction and washing device 40, a luminescent substrate liquid filling reaction and photon detection counting and data processing device 50 and a control center module (not shown in the figure).
An inner cavity is arranged in the shell; the sample processing device 10, the automatic sample feeding device 20, the dry integrated reagent access device 30, the integrated reagent immunoreaction and washing device 40, the luminescent substrate liquid filling reaction and photon detection counting and data processing device 50 are all arranged in the inner cavity and controlled by the control center module. The dry integrated reagent storage device 30 stores a plurality of dry integrated reagents, and the dry integrated reagents are covered with an integrated reagent sealing film 32222.
The sample processing device 10 comprises a centrifugation device 11, and the sample is automatically centrifuged under the control of the control center module. The sample tube to be tested after blood collection is directly put on a machine and centrifuged by a centrifugal device 11.
The autosampler 20 is controlled by the control center module to automatically add the centrifuged sample to the dry integrated reagent of the integrated reagent immunoreaction and washing device 40.
The dry type integrated reagent storing and taking device 30 stores a plurality of dry type integrated reagents, and is controlled by the control center module to automatically grasp the dry type integrated reagents into the integrated reagent immune reaction and washing device 40, and tear off the integrated reagent sealing film 32222.
The integrated reagent immunoreaction and washing apparatus 40 comprises an immunoreaction element and a washing element; the immunoreactive component comprises a dry marker solution filling device 42 for immunoreactions in the sample and the dry integrated reagent for detection; the washing element is controlled by the control center module to automatically wash the immunoreactive element.
The luminescent substrate liquid filling reaction and photon detection counting and data processing device 50 comprises a luminescent substrate liquid filling element and a photon detection counting and data processing element; the luminous substrate liquid filling element is controlled by the control center module to automatically fill luminous substrate liquid into the immunoreaction element for luminous reaction; the photon detection counting and data processing element is controlled by the control center module to automatically detect the luminescence reaction and report the detection result of the sample.
As shown in fig. 2 to 4, the sample processing device 10 includes a centrifugation device 11, a cover-opening grip 12, a sample tube holder 13, a sample tube transfer device 14, and a sample tray 15 for converting the whole blood centrifugation process of the sample tube into plasma/serum samples and for opening and transferring to the sample tray 15.
The centrifugal device 11 includes a centrifugal chamber 111, a centrifugal rotor 112, a first sample tube 113, a rotor core 115, and a first drive motor 116. The centrifuge rotor 112 is located within the centrifuge chamber 111, the first sample tube 113 is located on the centrifuge rotor 112, the rotor core 115 is located at the central axis of the centrifuge rotor 112, the rotor core 115 is electrically connected to the first drive motor 116, and the first drive motor 116 provides the driving force for centrifugation. The centrifugal cavity 111 limits the centrifugation process to the space where the centrifugal rotor 112 is located, the first sample tube position 113 is formed by arranging holes on the upper surface and the lower surface of the centrifugal rotor 112, and the sample tube is limited in the upper hole and the lower hole and is used for fixing the sample tube. The rotor core 115 is a dumbbell-shaped middle structure hollowed out in the middle part of the centrifugal rotor 112 so as to expose the sample tube body. The upper end of the centrifugal cavity 111 is open. The sidewall of the centrifugal cavity 111 is provided with a sample tube clamp hollow 114. A support rod is arranged beside the centrifugal device 11.
The cover opening clamping and lifting device 12 is arranged above the upper end opening of the centrifugal cavity 111; the cover opening clamp 12 comprises an automatic telescopic rod, wherein the lower end of the automatic telescopic rod is provided with an automatic clamp for clamping a tube cover of a sample tube, and the upper end of the automatic telescopic rod is fixedly connected to the top of the inner cavity of the shell.
One end of the sample tube clamp 13 is arranged on the support rod, and the other end is provided with an automatic clamp for clamping the tube wall of the sample tube and extends into a sample tube clamp hollow 114 on the side wall of the centrifugal cavity 111.
One end of the sample tube transferring device 14 is connected to the supporting rod, and can automatically rotate and automatically slide up and down along the supporting rod, and the other end is provided with an automatic clamp for clamping the sample tube.
The automatic clamp is of an openable arc clamping arm structure matched with the sample tube body.
The cover opening clamp 12 and the sample tube transferring device 14 can move up and down and left and right so as to facilitate the cover opening and the transfer of the sample tube into the sample tray 15.
The sample tray 15 includes a tray wall 151, a tray turntable 154, a radio frequency scanning identifier (not shown in the figure) and a second driving motor 155, the tray wall 151 is disposed on the tray turntable 154, a second sample tube position 152 is disposed on the tray wall 151, a sample tube identification hollow 153 is disposed on a side wall of the tray wall 151, the radio frequency scanning identifier scans and identifies sample tube information through the sample tube identification hollow 153, and the second driving motor 155 provides power for rotation of the sample tray 15.
The sample tube to be detected after blood collection is directly put on a machine, placed in a centrifugal device 11, the centrifugal device 11 loaded with the sample tube starts to centrifuge at a set rotating speed and time until the centrifugation is finished, then a sample tube clamp 13 stretches into the sample tube clamped in place from a sample tube clamp hollow hole 114, a cover opening clamp 12 clamps the outer edge of the bottom of the upper tube cover of the sample tube to lightly lift the cover, and the tube cover is discarded into a designated collecting container. The sample tube transfer apparatus 14 transfers the uncapped sample tube to the second sample tube position 152 designated by the sample tray 15, and then the radio frequency scanning identifier scans and identifies the sample tube information through the sample tube identification hollow 153, and transmits the identified sample tube information to the control center module, and allocates the detection items and sequences, and arranges the sample taking and adding volumes and sequences, the integrated reagent tube taking and loading sequences, and the tag solution adding volumes and sequences.
As shown in fig. 5, the automatic sampling device 20 includes a disposable tip 22 for sampling, a tip box 21 for storing the disposable tip, a driving sampling injector 23, and a slide 24 for driving the sampling injector 23 to move at different stations. The lower end of the driving sampling injector 23 is provided with a suction nozzle for inserting the suction nozzle; the upper end of the driving sampling injector 23 is connected with a sliding rod which is connected on the slideway 24 in a sliding way. The automatic sample feeding device 20 is controlled by the control center module to insert and take the disposable suction head 22 at the suction head box 21 according to a specified program, the drive sampling sample injector 23 is controlled to absorb a specified volume of sample, and the automatic sample feeding device moves at different stations along the slide rail 24 to complete the suction head insertion, the suction sample, the sample feeding, the uniform transfer of mixed samples and the disposal of the disposable suction head 22 into a specified container. The driving sampling injector 23 can move up and down and left and right in two dimensions, and can accurately suck and discharge samples. The embodiment of the invention adopts the disposable suction head for sampling, avoids carrying pollution and ensures that reactants are sufficiently uniform.
As shown in fig. 6, the dry integrated reagent access device 30 includes an integrated reagent storage structure 32, an integrated reagent holder push-pull structure 33, and a gripper and tear-off membrane robot 34.
As shown in fig. 7-8, the integrated reagent storage structure 32 includes an integrated reagent tray support cylinder 312, a plurality of integrated reagent tray trays 321 are disposed on the outer wall of the integrated reagent tray support cylinder 312 at equal intervals, integrated reagent tray slides 325 are disposed on the integrated reagent tray trays 321, integrated reagent trays 322 are disposed in the integrated reagent tray slides 325, integrated reagent trays 3223 are disposed in a plurality of integrated reagent plastic bodies 32223 loading positions, the integrated reagent trays 3223 are injection molded multi-position hollow structures, the integrated reagent plastic bodies 32223 include integrated reagent micro-reaction tubes 32211 and integrated reagent marker tubes 32213, the integrated reagent micro-reaction tubes 32211 and the integrated reagent marker tubes 32213 are in one-to-one correspondence injection molding, the bottom micro-reaction tubes 32211 are micro-structures such as various rotating bodies, pyramid bodies or platform bodies protruding upwards from the cavity, the integrated reagent micro-reaction tubes 32211 are filled with solid-phase immunoreaction reagents, the integrated reagent marker tubes 32213 are in cone-shaped structures, integrated reagent connecting pieces 32214 are plastic bodies, the integrated reagent connecting pieces 32214 are plastic bodies, the integrated reagent connecting pieces 32223 are independently connected with each other in a certain integrated reagent card integrated reagent forming stage 3222, and the integrated reagent connecting pieces 3222 are independently prepared in a certain integrated reagent connecting stage and integrated reagent connecting pieces 3222; the adjacent integrated reagent plastic bodies 32223 are completely broken at a certain stage of the preparation of the integrated reagent 3222 to form an integrated reagent separation slit 32221, and the integrated reagent micro-reaction tube 32211 and the integrated reagent marker tube 32213 are covered with an integrated reagent sealing film 32222.
The integrated reagent disk tray 321 of each layer is provided with a sector-shaped vacancy area which is equal to the sector where the integrated reagent disk 322 is positioned, and the sector-shaped vacancy area is a circular disk tray sector-shaped vacancy 324.
The bottom of the integrated reagent tray support cylinder 312 is provided with a liftable motor 311, the liftable motor 311 contains an electromagnetic material rotating arm, the integrated reagent tray support cylinder 312 is a central rotating shaft position of each layer of integrated reagent tray 321, a rotating inner ring on the integrated reagent tray 321 is an annular rotating slide block structure 323, a tray support 326 of the integrated reagent tray 321 is a sliding channel structure which is supported and limited on the outer wall of the integrated reagent tray support cylinder 312 under the cooperation of the rotating slide block 323, a permanent magnetic body is arranged at one position of the rotating slide block structure 23 and is matched with the electromagnetic material rotating arm of the liftable motor 311, the two parts are attracted after being electrified, the integrated reagent tray 321 is driven to rotate, and each layer of integrated reagent tray 321 independently rotates under the matching action of the electromagnetic material rotating arm of the lifting motor 311 of the rotating slide block structure 323 and the tray support 326, so as to obtain the target integrated reagent tray.
The bottom of the integrated reagent micro-reaction tube 32211 is formed into a micro-structure 32212 of the bottom of the integrated reagent micro-reaction tube by various rotating bodies, pyramid shapes or platform shapes protruding upwards in the cavity, so as to further increase the upward projection total surface area of the lower part of the micro-reaction tube, form a larger specific surface area, increase the light signal formed by projecting the reaction products upwards by the microtubules, and improve the sensitivity and the reaction speed.
The integrated reagent storage structure 32 is located within a barrel cylinder that is used to house and protect the integrated reagent tray and its contents and to cooperate with the ingress and egress of integrated reagents. The cylindrical inner wall 351 is slit-opened at one side of each integrated reagent disk tray 321 to form a barrel-shaped cylindrical wall slit 352, thereby loading the integrated reagent disk 322 and removing the integrated reagent holder 3223 for the used integrated reagent 3222. The top of the barrel-shaped cylinder is provided with a reaction plate 41 for the immune reaction to occur.
As shown in fig. 9, the integrated reagent seat push-pull structure 33 includes an integrated reagent seat push-pull slide 331, a scissors structure 334, an integrated reagent disk grabbing and pulling mechanism 335, an integrated reagent disk reciprocating slide motor 332, and a scissors structure driving motor 333. The scissors structure 334 is supported on the integrated reagent seat push-pull sliding slideway 331 through a supporting frame, and the integrated reagent disc grabbing and pulling mechanism 335 is arranged on the scissors structure 334. The scissors structure 334 is formed by intersecting two struts, the intersecting parts are connected by a scissors fork shaft 336, a sliding rod is arranged between the bottom ends of the two struts, a scissors structure driving motor 333 is arranged on the sliding rod, one end of the bottom of one of the two struts is driven by the scissors structure driving motor 333 to slide on the sliding rod, and the scissors structure 334 is driven to open and close, so that the integrated reagent tray grabbing and pulling mechanism 335 is lifted. The integrated reagent disk reciprocating sliding motor 332 drives the supporting frame to slide on the integrated reagent seat push-pull sliding slideway 331, so as to drive the whole integrated reagent seat push-pull structure 33 to slide. Sliding sleeves 337 are arranged at sliding positions of the sliding rods of the scissors structure 334 and the supporting frame so as to facilitate sliding. The integrated reagent disk grabbing and pulling mechanism 335 is a protruding structure at the top of the integrated reagent seat push-pull structure 33, and is inserted into the hollow structure at the bottom of the integrated reagent seat 3223, so as to push the integrated reagent disk 322 to slide in the integrated reagent disk slideway 325. A telescopic rod is arranged between the top ends of the two support rods, which is beneficial to opening and closing of the scissors structure 334.
When the integrated reagent 3222 is selected under the control of the control center module, the push-pull structure 33 of the integrated reagent seat slides and goes up and down, and rises from the circular tray sector 324 to push the integrated reagent tray 322 to be discharged into the integrated reagent tray 321, so as to grasp the integrated reagent 3222 by the grasping and placing integrated reagent and tearing the sealing film mechanical arm 34. The integrated reagent and seal tearing robot 34 is a combined mechanism whereby the integrated reagent 3222 is grasped and placed and the seal torn.
As shown in fig. 10, the mechanical arm 34 for gripping and releasing the integrated reagent and tearing the sealing film includes a cantilever beam 341, a traverse motor 342, a horizontal beam 343, a horizontal moving motor 344, a vertical moving motor 345, a vertical beam 3461, a cross beam 3462, an integrated reagent lifting and inserting motor 3463, an integrated reagent lifting and inserting vertical rod 3464, an integrated reagent lifting rod 3465, an integrated reagent pressing rod 3471, an integrated reagent pressing rod vertical 3472, an integrated reagent sealing film clamping and lifting motor 3473, an integrated reagent sealing film clamping and lifting rod 3474, and an integrated reagent sealing film clamping piece 3475.
The cantilever beam 341 is secured to the side wall or top structure of the housing with a slide thereon and carries the force of the pick and place integrated reagent and tear film mechanical arm 34 to self-weight and pick, place integrated reagent 3222 and tear integrated reagent seal film 32222. The horizontal beam 343 extends outwards from the axis of the integrated reagent tray 321 to form a parallel structure, one end of the horizontal beam 343 is connected to the suspension beam 341, and the traverse motor 342 is arranged at the connection between the horizontal beam 343 and the suspension beam 341. The traverse motor 342 drives the grasp and deposit integrated reagent and tear film mechanical arm 34 out into the integrated reagent tray receptacle 321. The other end of the horizontal beam 343 is connected to a vertical beam 3461, and a horizontal movement motor 344 is provided at the connection of the horizontal beam 343 and the vertical beam 3461, the horizontal movement motor 344 driving the horizontal beam 343 to approach or separate from the integrated reagent tray 321. A vertical travel motor 345 is provided below the horizontal travel motor 344 on the vertical beam 3461, and the vertical travel motor 345 drives the gripper and spreader integrated reagent and the tear film robot 34 to move up and down along the vertical beam 3461. The vertical beam 3461 carries the integrated reagent 3222 grasping and film tearing action. The cross member 3462 is a transversely extending structure in the middle of the vertical beam 3461 with an integrated reagent lift and insert motor 3463. One end of the integrated reagent lifting and inserting vertical rod 3464 is connected to the integrated reagent lifting and inserting motor 3463, and the other end is connected to the integrated reagent lifting and inserting rod 3465. The integrated reagent lift and insert motor 3463 drives the integrated reagent lift and insert vertical rod 3464 to approach or separate from the integrated reagent plastic body 32223. The integrated reagent lifting lever 3465 is a mechanism for lifting the integrated reagent plastic body 32223 off the integrated reagent holder 3223. The integrated reagent compression bar 3471 is a transverse structure perpendicular to the vertical beam 3461 and is located at the bottom of the vertical beam 3461. The integrated reagent presser bar 3471 assists in securing the integrated reagent 3222 as the integrated reagent lifter bar 3465 lifts the integrated reagent plastic body 32223 off the integrated reagent holder 3223 and provides support for the film tearing process as the integrated reagent plastic body 32223 is transferred to a defined station on the reaction tray 41. The integrated reagent compression bar upright 3472 is an upward extending structure at one end of the integrated reagent compression bar 3471, and provides support for the integrated reagent sealing membrane clamping motor 3473 and the lower structure thereof. One end of the integrated reagent sealing film clamping and lifting rod 3474 is connected to the integrated reagent pressing rod vertical 3472, the integrated reagent sealing film clamping and lifting motor 3473 is located at the joint of the integrated reagent sealing film clamping and lifting rod 3474 and the integrated reagent pressing rod vertical 3472, and the integrated reagent sealing film clamping and lifting motor 3473 drives the integrated reagent sealing film clamping and lifting rod 3474 to move up and down so as to clamp and release the integrated reagent sealing film 32222 and assist in tearing the film. The integrated reagent sealing membrane holder lifting rod 3474 is an upright structure connected to the integrated reagent sealing membrane holder lifting motor 3473, and extends laterally below the integrated reagent sealing membrane holder plate 3475. The integrated reagent sealing film clamping piece 3475 is driven by the integrated reagent sealing film clamping motor 3473 to move up and down to clamp and release the integrated reagent sealing film 32222, and the integrated reagent plastic body 32223 is grabbed and transferred to the corresponding station of the reaction disc 41 with the assistance of the horizontal moving motor 342, the horizontal moving motor 344, the vertical moving motor 345 and the integrated reagent lifting and inserting motor 3463, and the integrated reagent sealing film 32222 is torn off.
As shown in fig. 11, the integrated reagent immunoreaction device and washing device 40 comprises a reaction plate 41 and a driving motor thereof, a marker solution filling device 42, a washing head 43, a washing liquid filling and washing head inlet 431, a washing liquid filling micro-reaction tube outlet 432, a waste liquid extraction micro-reaction tube suction head 433, a waste liquid extraction and washing head outlet 434, a washing head cavity 435, a washing liquid filling device 44, a waste liquid pumping device 45 and a post-washing integrated reagent tube transfer arm 46.
The reaction plate 41 is provided at the top end of the dry integrated reagent access device 30. The reaction tray 41 is provided with a plurality of reaction stations for reaction of the integrated reagent 3222, a marker solution filling device 42 is arranged above the next station which is close to the loading station of the integrated reagent 3222, and more than 2 washing stations for washing the washing head 43 are arranged. The reaction disk 41 is driven to run according to a prescribed program, and the immune reaction can be timely washed and terminated to meet the optimal reaction time requirements of different projects. The reaction plate 41 is also provided with a sample feeding station for feeding samples by an automatic sample feeding device.
The marker solution filling device 42 includes a marker solution container, a motor driving the marker solution, a flow control valve, and a catheter. The label lysate filling apparatus 42 is configured to fill a defined volume of the lysate to dissolve the lyophilized label within the integrated reagent label tube 32213.
The wash liquid filling means 44 is a liquid container comprising a wash liquid container, a motor for driving the wash liquid, a liquid flow control valve and a liquid guide tube. The waste liquid pump 45 is composed of a waste liquid container, a motor for driving the waste liquid to be pumped, a liquid flow control valve and a liquid guide tube.
As shown in FIG. 12, the washing head 43 is a two-way liquid path structure connecting the washing liquid filling device 44 and the waste liquid pumping device 45, whereby the integrated reagent micro-reaction tube 32211 of the integrated reagent 3222 is washed sufficiently and thoroughly. The washing head 43 includes a washing chamber 435, a washing liquid filling washing head inlet 431, a washing liquid filling micro-reaction tube outlet 432, a waste liquid extraction micro-reaction tube suction head 433, and a waste liquid extraction washing head outlet 434. The wash liquid filling wash head inlet 431 communicates with the wash liquid filling device 44, and the wash liquid filling wash head inlet 431 communicates with the wash liquid filling microreactor tube outlet 432. The waste liquid extraction micro-reaction tube suction head 433 is communicated with a waste liquid extraction and washing outlet 434, and the waste liquid extraction and washing outlet 434 is communicated with a waste liquid extraction and discharge device 45. The washing liquid is injected into the washing head inlet to quantitatively introduce the washing liquid. The wash solution fill microreactor tube outlet 432 is directed into the integrated reagent microreactor tube 32211. The waste liquid pumping-out micro-reaction tube suction head 433 is a structure for pumping waste liquid, which is adaptive to the integrated reagent micro-reaction tube 32211, and the waste liquid pumping-out suction head is a horn-shaped structure with the bottom caliber larger than the tube diameter of the pumping-out tube. The waste liquid extraction rinse outlet 434 is the waste liquid exit suction rinse structure. The hair washing chamber 435 is a chamber channel in which the washing liquid and the waste liquid are partitioned, thereby uniformly introducing the washing liquid and discharging the waste liquid. The washing liquid filling device 44 and the waste liquid pumping device 45 are matched with the washing head 43, and can adopt continuous filling and pumping or intermittent filling or pumping waste liquid, so that a more efficient and rapid washing effect can be generated.
The post-wash integrated reagent tube transfer arm 46 includes a cross arm and an automatic gripping device attached to the cross arm. The post-washing integrated reagent tube transfer arm 46 is a means for transferring the post-washing reacted integrated reagent 3222 to the luminescence reaction tray 51.
The integrated reagent immunoreaction device and washing device 40 receive the grabbing and placing integrated reagent, and the film tearing mechanical arm 34 grabs and places the integrated reagent 3222 on the reaction disc reaction station, after the tearing of the sealing film is completed, the marker dissolved solution filling device 42 which is close to the integrated reagent 3222 is started, a specified volume of dissolved solution is filled after the integrated reagent 3222 enters the reaction station, and the integrated reagent is carried to the sample feeding station in a rotating way according to a specified flow. The automatic sampling device 20 inserts and takes a disposable suction head 22 at the suction head box 21 according to a designated program, sucks a sample with a designated volume under the control of the driving sampling sampler 23, and moves at different stations along the slide way 24 to complete the insertion of the suction head, the suction of the sample, the sample addition, the uniform transfer of the mixed sample and the disposal of the disposable suction head 22 into a designated container. And carrying to the first washing station by rotating according to a specified flow. The washing head 43 on the washing station is lowered to the upper edge of the integrated reagent 3222 micro-reaction tube 32211 to be butted, the waste liquid pumping device 45 is started first, the waste liquid pumping micro-reaction tube suction head 433 is submerged into the micro-reaction tube for pumping and discharging reaction liquid, then the washing liquid filling device 44 is started, the washing liquid is filled and guided into the integrated reagent micro-reaction tube 32211 through the micro-reaction tube outlet 432 in a directional manner, along the measuring wall and the bottom side flow, and the waste liquid is pumped and discharged through the waste liquid pumping micro-reaction tube suction head 433, and the waste liquid is led out of the head sucking and washing structure to a designated waste liquid container through the waste liquid pumping and washing outlet 434. The integrated reagent 3222 not washed at the first washing station is reacted in a certain flow path to the last washing station, and after washing according to the above washing flow path, the integrated reagent tube transfer arm 46 after washing is a device for transferring the integrated reagent 3222 after washing, which has reacted, to the luminescence reaction tray 51.
As shown in fig. 12-13, the luminescence substrate solution filling reaction and photon detection counting and data processing device 50 comprises a luminescence reaction tray 51, a luminescence substrate solution filling device 52, a photon detection counting and data processing device 53, and a test integrated reagent tube transfer arm 54.
The luminescent substrate liquid filling reaction and photon detection counting and data processing device 50 is a structural unit for filling luminescent substrate liquid, completing luminescent reaction and carrying out photon detection counting, data processing and result reporting. The luminescence reaction plate 51 has a plurality of stations for luminescence reaction and photon detection counting. The luminescent substrate liquid filling device 52 is a device comprising a luminescent liquid container, a motor for driving the luminescent liquid, a liquid flow control valve and a liquid guiding tube. The luminescent substrate liquid filling device 52 servo-fills a prescribed volume of luminescent substrate liquid. The photon detection count and data processing device 53 is a device for performing photon detection count, data qualitative and quantitative processing, and reporting results.
The post-test integrated reagent tube transfer arm 54 includes a cross arm and an automatic gripping device attached to the cross arm.
After washing, the reaction mixture is transferred to the integrated reagent 3222 transferred to the luminescence reaction plate 51, and the luminescence substrate solution filling device 52 fills a predetermined volume of luminescence substrate solution, and after a predetermined reaction time, the photon detection count and data processing device 53 performs photon detection count, qualitative and quantitative data processing, and reports the result. The post-test integrated reagent tube transfer arm 54 grips the post-reaction test integrated reagent tube and pours the luminescent substrate liquid into a designated waste liquid container and throws the non-liquid post-test integrated reagent tube into a designated waste collection container.
The integrated reagent micro-reaction tube 32211 is provided with a light shielding cover and a light guiding structure, the luminescence in the integrated reagent micro-reaction tube 32211 is projected to the photon detection count of the photomultiplier of the photon detection counting and data processing device 53, the photon detection counting device can convert the accumulated photon count in unit time in the integrated reagent micro-reaction tube 32211 into a relative luminescence intensity RLU value, and the standard curve is combined to perform qualitative or quantitative processing on the target object of the integrated reagent micro-reaction tube 32211 and report the result.
The full-automatic luminous immunoassay detector of the embodiment of the invention directly loads the sample tube to be detected after blood sampling into the machine, sequentially receives the automatic processing of the sample processing device 10, the automatic sample injection device 20, the dry integrated reagent access device 30, the integrated reagent immunoreaction and washing device 40, the luminous substrate liquid filling reaction and photon detection counting and data processing device 50 according to the flow, and then reports the result to realize full automation. After being checked by a checker, the system can intensively report all detection index results of the checked patient.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and various modifications and variations may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The full-automatic luminous immunoassay detector is characterized by comprising a shell, a sample processing device (10), an automatic sample injection device (20), a dry integrated reagent access device (30), an integrated reagent immunoreaction and washing device (40), a luminous substrate liquid filling reaction and photon detection counting and data processing device (50) and a control center module;
an inner cavity is arranged in the shell; the sample processing device (10), the automatic sample feeding device (20), the dry integrated reagent access device (30), the integrated reagent immune reaction and washing device (40), the luminescent substrate liquid filling reaction and photon detection counting and data processing device (50) are all arranged in the inner cavity and controlled by the control center module; a plurality of dry integrated reagents are stored on the dry integrated reagent storing and taking device (30), and an integrated reagent sealing film (32222) is covered on the dry integrated reagents;
the sample processing device (10) comprises a centrifugal device (11), and is controlled by the control center module to automatically perform centrifugal processing on a sample;
the automatic sample injection device (20) is controlled by the control center module to automatically add the centrifugally processed sample into the dry integrated reagent of the integrated reagent immunoreaction and washing device (40);
The dry integrated reagent storing and taking device (30) stores a plurality of dry integrated reagents, and is controlled by the control center module to automatically grab the dry integrated reagents into the integrated reagent immune reaction and washing device (40) and tear off an integrated reagent sealing film (32222);
the integrated reagent immunoreaction and wash apparatus (40) comprises an immunoreaction element and a wash element; the immunoreactive component comprises a dry marker solution filling device (42) for carrying out immunoreactions in the detection of the sample and the dry integrated reagent; the washing element is controlled by the control center module to automatically wash the immunoreaction element;
the luminous substrate liquid filling reaction and photon detection counting and data processing device (50) comprises a luminous substrate liquid filling element and a photon detection counting and data processing element; the luminous substrate liquid filling element is controlled by the control center module to automatically fill luminous substrate liquid into the immunoreaction element for luminous reaction; the photon detection counting and data processing element is controlled by the control center module to automatically detect the luminescence reaction and report the detection result of the sample.
2. The full-automatic luminescence immunoassay detector of claim 1, wherein the sample processing device (10) comprises a centrifugation device (11), a cover opening clamp (12), a sample tube clamp (13), a sample tube transfer device (14) and a sample introduction tray (15);
The centrifugal device (11) comprises a centrifugal cavity (111), a centrifugal rotor (112), a rotor core (115) and a first driving motor (116), wherein the upper end of the centrifugal cavity (111) is provided with an opening, a first sample tube position (113) is arranged in the centrifugal cavity, and a sample tube clamp hollow-out hole (114) is formed in the side wall of the centrifugal cavity; the rotor core (115) is a dumbbell-shaped middle structure hollowed out in the middle part of the centrifugal rotor (112) so as to expose the sample tube body; the driving motor (116) is used for generating power by centrifugation and providing power in the process of opening and transferring the sample tube;
the cover opening clamping and lifting device (12) is arranged above the upper end opening of the centrifugal cavity (111); the cover opening clamp handle (12) comprises an automatic telescopic rod, the lower end of the automatic telescopic rod is provided with an automatic clamp for clamping a tube cover of the sample tube, and the upper end of the automatic telescopic rod is fixedly connected to the top of the inner cavity of the shell;
a supporting rod is arranged beside the centrifugal device (11);
one end of the sample tube clamp (13) is arranged on the supporting rod, and the other end of the sample tube clamp is provided with an automatic clamp for clamping the tube wall of the sample tube and extends into a sample tube clamp hollow-out hole (114) on the side wall of the centrifugal cavity (111);
one end of the sample tube transferring device (14) is connected to the supporting rod and can automatically rotate and automatically slide up and down along the supporting rod, and the other end of the sample tube transferring device is provided with an automatic clamp for clamping a sample tube;
The sample injection disc (15) comprises a disc wall body (151), a chassis turntable (154), a radio frequency scanning identifier and a second driving motor (155); the tray wall body (151) is arranged on the tray turntable (154), a second sample tube position (152) is arranged on the tray wall body (151), a sample tube identification hollow-out hole (153) is formed in the side wall of the tray wall body (151), the radio frequency scanning identifier scans and identifies sample tube information through the sample tube identification hollow-out hole (153), and a second driving motor (155) provides power for rotation of the sample feeding tray (15).
3. The full-automatic luminescence immunoassay detector of claim 1, wherein the automatic sampling device (20) comprises a sampling tip (22), a tip box (21) for storing the tip, a driving sampling injector (23) and a slideway 24 for driving the sampling injector (23) to move at different stations; the lower end of the driving sampling injector (23) is provided with a suction nozzle for inserting the suction nozzle (22); the upper end of the driving sampling sampler (23) is connected with a slide bar which is connected on the slide way (24) in a sliding way.
4. The full-automatic luminescence immunoassay detector of claim 1, wherein the dry integrated reagent access device (30) comprises an integrated reagent storage structure (32), an integrated reagent seat push-pull structure (33) and a mechanical arm (34) for grasping and releasing integrated reagents and tearing a sealing film; the integrated reagent storage structure is used for storing integrated reagents, and the integrated reagent seat push-pull structure (33) and the grabbing and placing integrated reagents and the tearing and sealing film mechanical arm (34) are matched with each other to grab and place the dry integrated reagents (3222) on the integrated reagent storage structure (32) into the integrated reagent immunoreaction and washing device (40).
5. The full-automatic luminescence immunoassay analyzer of claim 4, wherein the integrated reagent storage structure comprises an integrated reagent tray support cylinder (312), a plurality of layers of equidistance integrated reagent tray supports (321) are arranged on the outer wall of the integrated reagent tray support cylinder (312), a plurality of integrated reagent tray slides (325) are arranged on the integrated reagent tray supports (321), integrated reagent trays (322) are arranged in the integrated reagent tray slides (325), a plurality of integrated reagent plastic bodies (32223) are arranged in an integrated reagent seat (3223) for loading, the integrated reagent seat (3223) is of an injection molding multiposition hollow structure, the integrated reagent plastic bodies (32223) comprise integrated reagent micro-reaction tubes (32211) and integrated reagent marker tubes (32213), the integrated reagent micro-reaction tube (32211) and the integrated reagent marker tube (32213) are in one-to-one correspondence injection molding, the micro structure (32212) at the bottom of the integrated reagent micro-reaction tube (32211) is a micro structure such as various rotating bodies, pyramid shapes or platform shapes protruding upwards from the cavity, the integrated reagent micro-reaction tube (32211) is provided with solid-phase immunoreaction reagent, the integrated reagent marker tube (32213) is internally provided with a cone-shaped tube structure, freeze-dried marker components are arranged in the integrated reagent tube, the integrated reagent connecting sheet (32214) is a plastic body, the integrated reagent connecting sheet (32214) connects a plurality of integrated reagent plastic bodies (32223) into a whole to form an integrated reagent strip, each integrated reagent plastic body (32223) of the integrated reagent strip is independently clamped and pressed on the integrated reagent seat (3223), and the integrated reagent connecting sheet (32214) is disconnected at a certain stage of integrated reagent (3222) preparation to form independent integrated reagents (3222); an integrated reagent separation slit (32221) is formed by completely breaking a certain step of the preparation of the integrated reagent (3222) between adjacent integrated reagent plastic bodies (32223), and an integrated reagent micro-reaction tube (32211) and an integrated reagent marker tube (32213) are covered by an integrated reagent sealing film (32222);
A sector-shaped vacancy area which is equal to the sector where the integrated reagent disk (322) is positioned is reserved on each layer of integrated reagent disk tray (321) and is a circular disk tray sector-shaped vacancy (324);
the bottom of the integrated reagent tray supporting cylinder (312) is provided with a liftable motor (311), the liftable motor (311) comprises an electromagnetic material rotating arm, the integrated reagent tray supporting cylinder (312) is a central rotating shaft position of each layer of integrated reagent tray supporting cylinder (321), a rotating inner ring on the integrated reagent tray supporting cylinder (321) is an annular rotating slide block structure (323), a tray supporting cylinder (326) of the integrated reagent tray supporting cylinder (321) is a sliding channel structure under the cooperation of the rotating slide block (323) supported and limited on the outer wall of the integrated reagent tray supporting cylinder (312), one part of the rotating slide block structure (323) is provided with a permanent magnetic body, the permanent magnetic body is matched with the electromagnetic material rotating arm of the liftable motor (311), the permanent magnetic body is attracted after being electrified, the permanent magnetic body drives the integrated reagent tray supporting cylinder (321) to rotate, and each layer of integrated reagent tray supporting cylinder (321) independently rotates under the electromagnetic material matching action of the lifting motor (311) of the rotating arm of the rotating slide block (323) and the tray supporting cylinder (326), and the target integrated reagent tray is obtained.
6. The full-automatic luminescence immunoassay monitor according to claim 4, wherein the integrated reagent pad push-pull structure (33) comprises an integrated reagent pad push-pull slide (331), a scissors structure (334), an integrated reagent pad grab-pull mechanism 335, the integrated reagent pad shuttle slide motor 332, a scissors structure drive motor 333; the integrated reagent tray grabbing and pulling mechanism (335) is arranged on the scissors structure (334); the scissors structure (334) is formed by crossing two struts, the crossing is connected by a scissors fork shaft (336), a sliding rod is arranged between the bottom ends of the two struts, a scissors structure driving motor (333) is arranged on the sliding rod, one end of the bottom of one of the two struts is driven by the scissors structure driving motor (333) to slide on the sliding rod, and then the scissors structure (334) is driven to open and close, so that the integrated reagent tray grabbing and pulling mechanism (335) is lifted, and the integrated reagent tray reciprocating sliding motor (332) drives the supporting frame to slide on the integrated reagent tray push-pull sliding slideway 331, so that the whole integrated reagent tray push-pull structure (33) is driven to slide.
7. The full-automatic luminescence immunoassay analyzer according to claim 4, wherein the integrated reagent holding and tearing and sealing mechanical arm (34) comprises a cantilever beam (341), a horizontal line motor (342), a horizontal beam (343), a horizontal moving motor (344), a vertical moving motor (345), a vertical beam (3461), a cross beam (3462), an integrated reagent lifting and inserting motor (3463), an integrated reagent lifting and inserting vertical rod (3464), an integrated reagent lifting rod (3465), an integrated reagent pressing rod (3471), an integrated reagent pressing rod standing (3472), an integrated reagent sealing film clamping and lifting motor (3473), an integrated reagent sealing film clamping and lifting rod (3474) and an integrated reagent sealing film clamping piece (3475); the cantilever beam (341) is fixed on the side wall or the top structure of the shell, a slide way is arranged on the cantilever beam (341), the horizontal beam (343) extends outwards from the axis of the integrated reagent tray (321) to form a parallel structure, one end of the horizontal beam (343) is connected to the cantilever beam (341), a traverse motor (342) is arranged at the joint of the horizontal beam (343) and the cantilever beam (341), the traverse motor (342) drives the integrated reagent to be placed and the film tearing mechanical arm 34 to be pulled out into the integrated reagent tray (321), the other end of the horizontal beam (343) is connected to a vertical beam 3461, a horizontal moving motor (344) is arranged at the joint of the horizontal beam (343) and the vertical beam (3461), the horizontal moving motor (344) drives the horizontal beam (343) to be close to or far away from the integrated reagent tray (321), a vertical moving motor (345) is arranged below the horizontal moving motor (344) on the vertical beam (3461), and the vertical moving motor (345) drives the integrated reagent to be placed and the film tearing mechanical arm 34 to move up and down along the vertical beam (3461); the cross beam (3462) is a transverse extension structure in the middle of the vertical beam (3461), and an integrated reagent lifting and inserting motor (3463) is arranged on the cross beam; one end of an integrated reagent lifting and inserting vertical rod (3464) is connected to an integrated reagent lifting and inserting motor (3463), and the other end is connected to an integrated reagent lifting rod (3465); an integrated reagent lifting and inserting motor (3463) drives an integrated reagent lifting and inserting vertical rod (3464) to be close to or separated from an integrated reagent plastic body (32223); the integrated reagent compression bar (3471) is of a transverse structure perpendicular to the vertical beam (3461) and is positioned at the bottom of the vertical beam (3461); the integrated reagent pressing rod is erected (3472) in an upward extending structure at one end of the integrated reagent pressing rod (3471), one end of the integrated reagent sealing film clamping lifting rod (3474) is connected to the integrated reagent pressing rod erected (3472), the integrated reagent sealing film clamping lifting motor (3473) is positioned at the joint of the integrated reagent sealing film clamping lifting rod (3474) and the integrated reagent pressing rod erected (3472), and the integrated reagent sealing film clamping lifting motor (3473) drives the integrated reagent sealing film clamping lifting rod (3474) to move up and down so as to clamp and release the integrated reagent sealing film (32222) and assist in tearing the film; the integrated reagent sealing membrane clamping lifting rod (3474) is an upright structure connected with the integrated reagent sealing membrane clamping lifting motor (3473), and extends transversely below the integrated reagent sealing membrane clamping lifting rod to form an integrated reagent sealing membrane clamping piece (3475).
8. The full-automatic luminescence immunoassay detector of claim 1, wherein the integrated reagent immunoreaction and washing device (40) comprises a reaction plate (41) and a driving motor thereof, a marker solution filling device (42), a hair washing device (43), a washing liquid filling device (44), a waste liquid pumping device (45) and a post-washing integrated reagent tube transfer arm (46); the reaction disk (41) is arranged at the top end of the dry integrated reagent access device (30); the reaction tray (41) is provided with a plurality of reaction stations for the reaction of the integrated reagent (3222), a marker solution filling device (42) is arranged above the next station which is close to the loading station of the integrated reagent (3222), and more than 2 washing stations for washing the washing heads (43) are arranged; a sample injection station is also arranged on the reaction disc (41) for the automatic sample injection device to feed samples;
the marker solution filling device (42) comprises a marker solution container, a motor for driving the marker solution, a liquid flow control valve and a liquid guide tube;
the lotion-filling device (44) is a container containing lotion, a motor for driving the lotion, a flow control valve and a catheter; the waste liquid pumping and draining device (45) comprises a waste liquid container, a motor for driving waste liquid to be pumped, a liquid flow control valve and a liquid guide pipe;
The post-wash integrated reagent tube transfer arm (46) includes a cross arm and an automatic gripping device attached to the cross arm.
9. The full-automatic luminescence immunoassay detector of claim 8, wherein the wash head (43) comprises a wash head cavity (435), a wash liquid filling wash head inlet (431), a wash liquid filling micro-reaction tube outlet (432), a waste liquid extraction micro-reaction tube tip (433), a waste liquid extraction wash head outlet (434); a wash liquor filling and washing head inlet (431) is communicated with a wash liquor filling device (44), and the wash liquor filling and washing head inlet (431) is communicated with a wash liquor filling micro-reaction tube outlet (432); the waste liquid extraction micro-reaction tube suction head (433) is communicated with a waste liquid extraction and washing outlet (434), and the waste liquid extraction and washing outlet (434) is communicated with a waste liquid extraction and discharge device (45); a wash liquid filling wash head inlet (431) for quantitatively introducing a wash liquid, and a wash liquid filling micro-reaction tube outlet (432) for guiding the wash liquid into the integrated reagent micro-reaction tube (32211) in a directional manner; the waste liquid sucking head (433) of the waste liquid sucking micro-reaction tube is a structure for sucking waste liquid, which is adaptive to the integrated reagent micro-reaction tube (32211), and the waste liquid sucking head for sucking and discharging is a horn-shaped structure with the bottom caliber larger than the tube diameter of the sucking tube; the hair washing cavity (435) is a cavity channel separated by the internal washing liquid and the waste liquid.
10. The full-automatic luminescent immunoassay detector of claim 1, wherein the luminescent substrate liquid filling reaction and photon detection counting and data processing device (50) comprises a luminescent reaction disc (51), a luminescent substrate liquid filling device (52), a photon detection counting and data processing device (53) and a test integrated reagent tube transfer arm (54);
A plurality of stations are arranged on the luminous reaction disk (51) for luminous reaction and photon detection counting; the luminous substrate liquid filling device (52) comprises a luminous liquid container, a motor for driving luminous liquid, a liquid flow control valve and a liquid guide tube; the post-test integrated reagent tube transfer arm (54) includes a cross arm and an automatic gripping device connected to the cross arm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310889485.3A CN117054673A (en) | 2023-07-19 | 2023-07-19 | Full-automatic luminous immunoassay detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310889485.3A CN117054673A (en) | 2023-07-19 | 2023-07-19 | Full-automatic luminous immunoassay detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117054673A true CN117054673A (en) | 2023-11-14 |
Family
ID=88668230
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310889485.3A Pending CN117054673A (en) | 2023-07-19 | 2023-07-19 | Full-automatic luminous immunoassay detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117054673A (en) |
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2023
- 2023-07-19 CN CN202310889485.3A patent/CN117054673A/en active Pending
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