CN117042810A - 用于具有提高的感染性的aav病毒颗粒的aav产生系统 - Google Patents
用于具有提高的感染性的aav病毒颗粒的aav产生系统 Download PDFInfo
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- CN117042810A CN117042810A CN202280022124.2A CN202280022124A CN117042810A CN 117042810 A CN117042810 A CN 117042810A CN 202280022124 A CN202280022124 A CN 202280022124A CN 117042810 A CN117042810 A CN 117042810A
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- Prior art keywords
- aav
- raav
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- capsid
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- 239000002245 particle Substances 0.000 title abstract description 196
- 230000003612 virological effect Effects 0.000 title abstract description 136
- 238000004519 manufacturing process Methods 0.000 title description 85
- 230000001965 increasing effect Effects 0.000 title description 40
- 210000000234 capsid Anatomy 0.000 claims abstract description 309
- 238000000034 method Methods 0.000 claims abstract description 135
- 101000805768 Banna virus (strain Indonesia/JKT-6423/1980) mRNA (guanine-N(7))-methyltransferase Proteins 0.000 claims description 134
- 101000686790 Chaetoceros protobacilladnavirus 2 Replication-associated protein Proteins 0.000 claims description 134
- 101000864475 Chlamydia phage 1 Internal scaffolding protein VP3 Proteins 0.000 claims description 134
- 101000803553 Eumenes pomiformis Venom peptide 3 Proteins 0.000 claims description 134
- 101000583961 Halorubrum pleomorphic virus 1 Matrix protein Proteins 0.000 claims description 134
- 101710081079 Minor spike protein H Proteins 0.000 claims description 121
- 241000238631 Hexapoda Species 0.000 claims description 104
- 229910052723 transition metal Inorganic materials 0.000 claims description 78
- 150000003624 transition metals Chemical class 0.000 claims description 76
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 70
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 69
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 44
- 229910052802 copper Inorganic materials 0.000 claims description 44
- 239000010949 copper Substances 0.000 claims description 44
- 238000012258 culturing Methods 0.000 claims description 43
- 238000010348 incorporation Methods 0.000 claims description 42
- 241000702421 Dependoparvovirus Species 0.000 claims description 17
- 101000749287 Clitocybe nebularis Clitocypin Proteins 0.000 claims description 13
- 101000767029 Clitocybe nebularis Clitocypin-1 Proteins 0.000 claims description 13
- 229940094664 Cysteine protease inhibitor Drugs 0.000 claims description 13
- -1 transition metal salt Chemical class 0.000 claims description 10
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- XTVVROIMIGLXTD-UHFFFAOYSA-N copper(II) nitrate Chemical compound [Cu+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XTVVROIMIGLXTD-UHFFFAOYSA-N 0.000 claims description 8
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 7
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 6
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims description 6
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 5
- 235000019800 disodium phosphate Nutrition 0.000 claims description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 5
- 229910052737 gold Inorganic materials 0.000 claims description 5
- 239000010931 gold Substances 0.000 claims description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 claims description 4
- KQHXBDOEECKORE-UHFFFAOYSA-L beryllium sulfate Chemical compound [Be+2].[O-]S([O-])(=O)=O KQHXBDOEECKORE-UHFFFAOYSA-L 0.000 claims description 4
- 229910021538 borax Inorganic materials 0.000 claims description 4
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 4
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 4
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims description 4
- HSZCZNFXUDYRKD-UHFFFAOYSA-M lithium iodide Chemical compound [Li+].[I-] HSZCZNFXUDYRKD-UHFFFAOYSA-M 0.000 claims description 4
- IIPYXGDZVMZOAP-UHFFFAOYSA-N lithium nitrate Chemical compound [Li+].[O-][N+]([O-])=O IIPYXGDZVMZOAP-UHFFFAOYSA-N 0.000 claims description 4
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical compound [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- NROKBHXJSPEDAR-UHFFFAOYSA-M potassium fluoride Chemical compound [F-].[K+] NROKBHXJSPEDAR-UHFFFAOYSA-M 0.000 claims description 4
- 229910052709 silver Inorganic materials 0.000 claims description 4
- 239000004332 silver Substances 0.000 claims description 4
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 claims description 4
- 239000004328 sodium tetraborate Substances 0.000 claims description 4
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 4
- DHEQXMRUPNDRPG-UHFFFAOYSA-N strontium nitrate Chemical compound [Sr+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O DHEQXMRUPNDRPG-UHFFFAOYSA-N 0.000 claims description 4
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 4
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 3
- JJLJMEJHUUYSSY-UHFFFAOYSA-L Copper hydroxide Chemical compound [OH-].[OH-].[Cu+2] JJLJMEJHUUYSSY-UHFFFAOYSA-L 0.000 claims description 3
- 239000005750 Copper hydroxide Substances 0.000 claims description 3
- QPLDLSVMHZLSFG-UHFFFAOYSA-N Copper oxide Chemical compound [Cu]=O QPLDLSVMHZLSFG-UHFFFAOYSA-N 0.000 claims description 3
- 239000005751 Copper oxide Substances 0.000 claims description 3
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 3
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 claims description 3
- DOVLHZIEMGDZIW-UHFFFAOYSA-N [Cu+3].[O-]B([O-])[O-] Chemical compound [Cu+3].[O-]B([O-])[O-] DOVLHZIEMGDZIW-UHFFFAOYSA-N 0.000 claims description 3
- RAOSIAYCXKBGFE-UHFFFAOYSA-K [Cu+3].[O-]P([O-])([O-])=O Chemical compound [Cu+3].[O-]P([O-])([O-])=O RAOSIAYCXKBGFE-UHFFFAOYSA-K 0.000 claims description 3
- 229910052793 cadmium Inorganic materials 0.000 claims description 3
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims description 3
- 229910052804 chromium Inorganic materials 0.000 claims description 3
- 239000011651 chromium Substances 0.000 claims description 3
- 229910017052 cobalt Inorganic materials 0.000 claims description 3
- 239000010941 cobalt Substances 0.000 claims description 3
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 3
- 229940116318 copper carbonate Drugs 0.000 claims description 3
- 229910001956 copper hydroxide Inorganic materials 0.000 claims description 3
- 229910000431 copper oxide Inorganic materials 0.000 claims description 3
- GEZOTWYUIKXWOA-UHFFFAOYSA-L copper;carbonate Chemical compound [Cu+2].[O-]C([O-])=O GEZOTWYUIKXWOA-UHFFFAOYSA-L 0.000 claims description 3
- ZZBBCSFCMKWYQR-UHFFFAOYSA-N copper;dioxido(oxo)silane Chemical compound [Cu+2].[O-][Si]([O-])=O ZZBBCSFCMKWYQR-UHFFFAOYSA-N 0.000 claims description 3
- 229910052735 hafnium Inorganic materials 0.000 claims description 3
- VBJZVLUMGGDVMO-UHFFFAOYSA-N hafnium atom Chemical compound [Hf] VBJZVLUMGGDVMO-UHFFFAOYSA-N 0.000 claims description 3
- 229910052741 iridium Inorganic materials 0.000 claims description 3
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 claims description 3
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 3
- 229910052753 mercury Inorganic materials 0.000 claims description 3
- 229910052759 nickel Inorganic materials 0.000 claims description 3
- 229910052762 osmium Inorganic materials 0.000 claims description 3
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 claims description 3
- 229910052763 palladium Inorganic materials 0.000 claims description 3
- 229910052697 platinum Inorganic materials 0.000 claims description 3
- 229910052702 rhenium Inorganic materials 0.000 claims description 3
- WUAPFZMCVAUBPE-UHFFFAOYSA-N rhenium atom Chemical compound [Re] WUAPFZMCVAUBPE-UHFFFAOYSA-N 0.000 claims description 3
- 229910052703 rhodium Inorganic materials 0.000 claims description 3
- 239000010948 rhodium Substances 0.000 claims description 3
- MHOVAHRLVXNVSD-UHFFFAOYSA-N rhodium atom Chemical compound [Rh] MHOVAHRLVXNVSD-UHFFFAOYSA-N 0.000 claims description 3
- 229910052706 scandium Inorganic materials 0.000 claims description 3
- SIXSYDAISGFNSX-UHFFFAOYSA-N scandium atom Chemical compound [Sc] SIXSYDAISGFNSX-UHFFFAOYSA-N 0.000 claims description 3
- IRPLSAGFWHCJIQ-UHFFFAOYSA-N selanylidenecopper Chemical compound [Se]=[Cu] IRPLSAGFWHCJIQ-UHFFFAOYSA-N 0.000 claims description 3
- 239000001488 sodium phosphate Substances 0.000 claims description 3
- 229910052715 tantalum Inorganic materials 0.000 claims description 3
- GUVRBAGPIYLISA-UHFFFAOYSA-N tantalum atom Chemical compound [Ta] GUVRBAGPIYLISA-UHFFFAOYSA-N 0.000 claims description 3
- 229910052719 titanium Inorganic materials 0.000 claims description 3
- 239000010936 titanium Substances 0.000 claims description 3
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 3
- 229910052721 tungsten Inorganic materials 0.000 claims description 3
- 239000010937 tungsten Substances 0.000 claims description 3
- 229910052720 vanadium Inorganic materials 0.000 claims description 3
- 229910052727 yttrium Inorganic materials 0.000 claims description 3
- VWQVUPCCIRVNHF-UHFFFAOYSA-N yttrium atom Chemical compound [Y] VWQVUPCCIRVNHF-UHFFFAOYSA-N 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 229910052726 zirconium Inorganic materials 0.000 claims description 3
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 claims description 2
- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical compound C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 claims description 2
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- UNMYWSMUMWPJLR-UHFFFAOYSA-L Calcium iodide Chemical compound [Ca+2].[I-].[I-] UNMYWSMUMWPJLR-UHFFFAOYSA-L 0.000 claims description 2
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 2
- 239000004115 Sodium Silicate Substances 0.000 claims description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- 239000004288 Sodium dehydroacetate Substances 0.000 claims description 2
- VEMHGRSPAZPNBH-UHFFFAOYSA-N [Ru].[Tc] Chemical compound [Ru].[Tc] VEMHGRSPAZPNBH-UHFFFAOYSA-N 0.000 claims description 2
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 claims description 2
- QAFATHSWTJYDJP-UHFFFAOYSA-J aluminum;rubidium(1+);disulfate Chemical compound [Al+3].[Rb+].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O QAFATHSWTJYDJP-UHFFFAOYSA-J 0.000 claims description 2
- YPPRKFXMPDSDJV-UHFFFAOYSA-J aluminum;sodium;2-hydroxypropanoate Chemical compound [Na+].[Al+3].CC(O)C([O-])=O.CC(O)C([O-])=O.CC(O)C([O-])=O.CC(O)C([O-])=O YPPRKFXMPDSDJV-UHFFFAOYSA-J 0.000 claims description 2
- RQPZNWPYLFFXCP-UHFFFAOYSA-L barium dihydroxide Chemical compound [OH-].[OH-].[Ba+2] RQPZNWPYLFFXCP-UHFFFAOYSA-L 0.000 claims description 2
- 229910001863 barium hydroxide Inorganic materials 0.000 claims description 2
- 229910001622 calcium bromide Inorganic materials 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 235000011148 calcium chloride Nutrition 0.000 claims description 2
- WGEFECGEFUFIQW-UHFFFAOYSA-L calcium dibromide Chemical compound [Ca+2].[Br-].[Br-] WGEFECGEFUFIQW-UHFFFAOYSA-L 0.000 claims description 2
- 229940046413 calcium iodide Drugs 0.000 claims description 2
- 229910001640 calcium iodide Inorganic materials 0.000 claims description 2
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 2
- 229940059936 lithium bromide Drugs 0.000 claims description 2
- PEXNRZDEKZDXPZ-UHFFFAOYSA-N lithium selenidolithium Chemical compound [Li][Se][Li] PEXNRZDEKZDXPZ-UHFFFAOYSA-N 0.000 claims description 2
- OTCKOJUMXQWKQG-UHFFFAOYSA-L magnesium bromide Chemical compound [Mg+2].[Br-].[Br-] OTCKOJUMXQWKQG-UHFFFAOYSA-L 0.000 claims description 2
- 229910001623 magnesium bromide Inorganic materials 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 229910052750 molybdenum Inorganic materials 0.000 claims description 2
- 239000011733 molybdenum Substances 0.000 claims description 2
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 2
- LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 claims description 2
- 229910000363 nickel(II) sulfate Inorganic materials 0.000 claims description 2
- 229910052758 niobium Inorganic materials 0.000 claims description 2
- 239000010955 niobium Substances 0.000 claims description 2
- GUCVJGMIXFAOAE-UHFFFAOYSA-N niobium atom Chemical compound [Nb] GUCVJGMIXFAOAE-UHFFFAOYSA-N 0.000 claims description 2
- GRLPQNLYRHEGIJ-UHFFFAOYSA-J potassium aluminium sulfate Chemical compound [Al+3].[K+].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O GRLPQNLYRHEGIJ-UHFFFAOYSA-J 0.000 claims description 2
- 239000011698 potassium fluoride Substances 0.000 claims description 2
- 235000003270 potassium fluoride Nutrition 0.000 claims description 2
- 229940096017 silver fluoride Drugs 0.000 claims description 2
- REYHXKZHIMGNSE-UHFFFAOYSA-M silver monofluoride Chemical compound [F-].[Ag+] REYHXKZHIMGNSE-UHFFFAOYSA-M 0.000 claims description 2
- 239000001632 sodium acetate Substances 0.000 claims description 2
- 235000017281 sodium acetate Nutrition 0.000 claims description 2
- 229960004249 sodium acetate Drugs 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 239000001509 sodium citrate Substances 0.000 claims description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 2
- 229940079839 sodium dehydroacetate Drugs 0.000 claims description 2
- 235000019259 sodium dehydroacetate Nutrition 0.000 claims description 2
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 claims description 2
- 239000000176 sodium gluconate Substances 0.000 claims description 2
- 235000012207 sodium gluconate Nutrition 0.000 claims description 2
- 229940005574 sodium gluconate Drugs 0.000 claims description 2
- 229940073490 sodium glutamate Drugs 0.000 claims description 2
- 239000001540 sodium lactate Substances 0.000 claims description 2
- 235000011088 sodium lactate Nutrition 0.000 claims description 2
- 229940005581 sodium lactate Drugs 0.000 claims description 2
- 235000019795 sodium metasilicate Nutrition 0.000 claims description 2
- BYKRNSHANADUFY-UHFFFAOYSA-M sodium octanoate Chemical compound [Na+].CCCCCCCC([O-])=O BYKRNSHANADUFY-UHFFFAOYSA-M 0.000 claims description 2
- ZNCPFRVNHGOPAG-UHFFFAOYSA-L sodium oxalate Chemical compound [Na+].[Na+].[O-]C(=O)C([O-])=O ZNCPFRVNHGOPAG-UHFFFAOYSA-L 0.000 claims description 2
- 229940039790 sodium oxalate Drugs 0.000 claims description 2
- 229940048086 sodium pyrophosphate Drugs 0.000 claims description 2
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 2
- 235000011152 sodium sulphate Nutrition 0.000 claims description 2
- DSOWAKKSGYUMTF-GZOLSCHFSA-M sodium;(1e)-1-(6-methyl-2,4-dioxopyran-3-ylidene)ethanolate Chemical compound [Na+].C\C([O-])=C1/C(=O)OC(C)=CC1=O DSOWAKKSGYUMTF-GZOLSCHFSA-M 0.000 claims description 2
- JOWTZKYGHSPYKA-UHFFFAOYSA-M sodium;2-(2-butoxyethoxy)acetate Chemical compound [Na+].CCCCOCCOCC([O-])=O JOWTZKYGHSPYKA-UHFFFAOYSA-M 0.000 claims description 2
- QSGQSSCOSSIDLZ-UHFFFAOYSA-M sodium;2-(dihydroxyamino)acetate Chemical compound [Na+].ON(O)CC([O-])=O QSGQSSCOSSIDLZ-UHFFFAOYSA-M 0.000 claims description 2
- UOULCEYHQNCFFH-UHFFFAOYSA-M sodium;hydroxymethanesulfonate Chemical compound [Na+].OCS([O-])(=O)=O UOULCEYHQNCFFH-UHFFFAOYSA-M 0.000 claims description 2
- YJPVTCSBVRMESK-UHFFFAOYSA-L strontium bromide Chemical compound [Br-].[Br-].[Sr+2] YJPVTCSBVRMESK-UHFFFAOYSA-L 0.000 claims description 2
- 229910001625 strontium bromide Inorganic materials 0.000 claims description 2
- 229940074155 strontium bromide Drugs 0.000 claims description 2
- 229910001631 strontium chloride Inorganic materials 0.000 claims description 2
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 claims description 2
- UUCCCPNEFXQJEL-UHFFFAOYSA-L strontium dihydroxide Chemical compound [OH-].[OH-].[Sr+2] UUCCCPNEFXQJEL-UHFFFAOYSA-L 0.000 claims description 2
- 229910001866 strontium hydroxide Inorganic materials 0.000 claims description 2
- KRIJWFBRWPCESA-UHFFFAOYSA-L strontium iodide Chemical compound [Sr+2].[I-].[I-] KRIJWFBRWPCESA-UHFFFAOYSA-L 0.000 claims description 2
- 229910001643 strontium iodide Inorganic materials 0.000 claims description 2
- 235000019818 tetrasodium diphosphate Nutrition 0.000 claims description 2
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 claims description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 2
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Abstract
本发明提供了经修饰的α病毒以及用于制备和使用特别是用衣壳假型化的AAV病毒颗粒的组合物、方法和试剂盒，特别是用于基因疗法和/或诊断。
Description
序列表的引用
创建于2021年2月1日并且大小为475千字节(486,939字节)的以命名为“2021-02-01_Sequence-Listing_6439-0114PUS1_ST25”的文本文件与此同时提交的序列表根据37C.F.R.§1.52(e)(5)通过引用并入本文。
技术领域
本发明涉及用于制备腺相关病毒(AAV)病毒颗粒的方法。本发明还涉及本文描述的AAV病毒颗粒以及与其相关的组合物、方法、用途和试剂盒。
背景技术
腺相关病毒(AAV)是据信需要辅助腺病毒进行复制的小型非致病性卫星病毒。AAV在结构上与腺病毒类似，但具有较小的二十面体核衣壳。AAV是具有在末端有至少一个反向末端重复序列(ITR)的单链DNA基因组的无包膜病毒。例如，AAV2血清型可以具有在末端有两个145个核苷酸长的ITR的、大约4.7千碱基(kb)的单链DNA基因组。病毒不编码聚合酶并且因此依赖细胞聚合酶进行基因组复制。ITR侧接分别编码非结构蛋白和结构蛋白的两个病毒基因—rep(复制)和cap(衣壳)。rep基因通过使用两个启动子和替代性剪接来编码被称为Rep78、Rep68、Rep52和Rep40的四种调节蛋白。这些蛋白质参与AAV基因组复制和包装。cap基因通过替代性剪接和启动翻译来产生三种衣壳蛋白VP1(病毒粒子蛋白1)、VP2和VP3。AAV2的VP1、VP2和VP3的分子量分别为87、72和62kDa。这些衣壳蛋白组装成60个亚基的近球形蛋白壳。AAV结构简单性和非致病性质使重组AAV(rAAV)成为有用的基因疗法载体。AAV基因疗法载体可以感染复制细胞和非复制细胞两者并引入转基因，而无需整合到宿主细胞的基因组中。rAAV载体常常因其高滴度、感染广泛范围的细胞的能力、温和的免疫应答以及整体安全性而是优选的。已经发现rAAV基因疗法载体对于许多疾病(包括糖尿病和其他胰腺病症)非常有用。
仍然需要新的且有效的AAV病毒颗粒以及与其相关的组合物、方法和试剂盒。
发明内容
在各个方面，公开了用于制备重组腺相关病毒(rAAV)的方法。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有有效量的过渡金属的培养基中培养的步骤。宿主细胞能够产生rAAV衣壳。过渡金属的有效量增加VP1、VP2或VP3蛋白到rAAV衣壳中的掺入。替代性地，过渡金属减少VP2的掺入并增加VP1或VP3到rAAV衣壳中的掺入。过渡金属还可以减少VP3的掺入并增加VP1到rAAV衣壳中的掺入。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有纳摩尔浓度的过渡金属的培养基中培养宿主细胞的步骤。宿主细胞能够产生rAAV衣壳。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有微摩尔浓度的过渡金属的培养基中培养宿主细胞的步骤。宿主细胞能够产生rAAV衣壳。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有有效量的过渡金属的培养基中培养的步骤。宿主细胞能够产生rAAV衣壳。过渡金属的有效量增加VP1和VP3蛋白到rAAV衣壳中的掺入。rAAV衣壳具有大于在相同条件下但缺乏有效量的过渡金属的情况下产生的rAAV衣壳的VP1和VP3蛋白浓度的VP1和VP3蛋白浓度。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有有效量的过渡金属的培养基中培养的步骤。宿主细胞能够产生rAAV衣壳。过渡金属的有效量增加VP1蛋白到rAAV衣壳中的掺入。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有有效量的半胱氨酸蛋白酶抑制剂的培养基中培养的步骤。宿主细胞能够产生rAAV衣壳。半胱氨酸蛋白酶抑制剂的有效量增加VP1、VP2或VP3蛋白到rAAV衣壳中的掺入。替代性地，半胱氨酸蛋白酶抑制剂的有效量减少VP2的掺入并增加VP1或VP3到rAAV衣壳中的掺入。半胱氨酸蛋白酶抑制剂的有效量还可以减少VP3的掺入并增加VP1到rAAV衣壳中的掺入。
一方面，公开了一种通过任何方面或实施例的任何方法产生的rAAV衣壳。rAAV衣壳具有大于在相同条件下但缺乏有效量的过渡金属的情况下产生的rAAV衣壳的VP1、VP2或VP3蛋白浓度的VP1、VP2或VP3蛋白浓度。
一方面，公开了一种通过任何方面或实施例的任何方法产生的rAAV衣壳。rAAV衣壳具有低于在相同条件下但缺乏有效量的过渡金属的情况下产生的rAAV衣壳的VP2或VP3蛋白浓度的VP2或VP3蛋白浓度。
一方面，公开了一种通过任何方面或实施例的任何方法产生的rAAV衣壳。rAAV衣壳表现出增强的感染性。
一方面，通过任何方面或实施例的任何方法产生的rAAV衣壳是治疗有效的rAAV。
在一个实施例中，宿主细胞是非哺乳动物宿主细胞。在另一个实施例中，宿主细胞是昆虫细胞。
在一个实施例中，过渡金属是过渡金属盐。过渡金属的实例包括乙酸铜、氧化亚铜、氧化铜、氯化铜、氯氧化铜、氯化亚铜、硝酸铜、氰化铜、铜皂、环烷酸铜或硫酸铜。
在各种实施例中，过渡金属选自以下中的一者或多者：铜、钪、钛、钒、铬、锰、铁、钴、镍、铜、锌、钇、锆、铌、钼、锝、钌、铑、钯、银、镉、铪、钽、钨、铼、锇、铱、铂、金、汞、以及/>
在各种实施例中，过渡金属的有效量的范围为约1纳摩尔(nM)至约1毫摩尔(mM)。在各种实施例中，过渡金属的有效量的范围为约10nM至约100微摩尔(μM)。在各种实施例中，过渡金属的有效量的范围为约20μM至约25μM。在各种实施例中，过渡金属的纳摩尔浓度的范围为约1nM至小于1μM。
在各种实施例中，过渡金属的纳摩尔浓度的范围为约10nM至小于1μM。
在各种实施例中，过渡金属的微摩尔浓度的范围为约1μM至小于1mM。在各种实施例中，过渡金属的微摩尔浓度的范围为约1μM至约100μM。在各种实施例中，过渡金属的微摩尔浓度的范围为约20μM至约25μM。
在各种实施例中，过渡金属的有效量足以增加VP1蛋白到rAAV衣壳中的掺入。在各种实施例中，过渡金属的有效量足以增加或减少VP3蛋白到rAAV衣壳中的掺入。在各种实施例中，过渡金属的有效量足以增加或减少VP2蛋白到rAAV衣壳中的掺入。在各种实施例中，过渡金属的有效量足以增加VP1、VP2或VP3蛋白到rAAV衣壳中的掺入。在其他实施例中，过渡金属的有效量足以减少VP2或VP3蛋白到rAAV衣壳中的掺入。
在各种实施例中，任何方面或实施例的方法进一步包括从宿主细胞中分离rAAV衣壳的步骤。
在各种实施例中，任何方面或实施例的方法进一步包括能够产生rAAV衣壳的宿主细胞。
在各种实施例中，任何方面或实施例的培养步骤在至少1升(L)、至少10L、至少50L、至少100L、至少250L、至少500L、至少1000L、至少1500L、至少2000L或至少2500L的体积中进行。
一方面，本发明提供了一种用于制备AAV病毒颗粒的方法。该方法包括在包含有效量的盐的培养基中培养能够产生AAV病毒颗粒的宿主细胞。AAV病毒颗粒包含AAV衣壳。AAV衣壳包含VP1蛋白。盐包含过渡金属。
另一方面，本发明提供了一种用于制备AAV病毒颗粒的方法。该方法包括在具有有效量的半胱氨酸蛋白酶抑制剂的培养基中培养能够产生AAV病毒颗粒的宿主细胞，其中AAV病毒颗粒包含AAV衣壳并且AAV衣壳包含VP1蛋白。
在其他方面，本发明提供了一种治疗有效的rAAV。治疗有效的rAAV通过任何方面或任何实施例的任何方法产生。治疗有效的rAAV用AAV衣壳假型化。
在一些方面，本发明提供了一种rAAV颗粒，其通过包括在含有有效量的盐的培养基中培养能够产生AAV病毒颗粒的宿主细胞的方法来制备。AAV病毒颗粒包含AAV衣壳。AAV衣壳包含VP1蛋白。盐包含过渡金属诸如铜盐。
一方面，本发明提供了一种rAAV颗粒，其通过包括在具有有效量的半胱氨酸蛋白酶抑制剂的培养基中培养能够产生rAAV颗粒的宿主细胞的方法来制备。rAAV颗粒包含AAV衣壳。AAV衣壳包含VP1蛋白。
另一方面，本发明提供了本发明的rAAV颗粒的药物调配物。
在一些方面，本发明提供了本发明的rAAV颗粒用于高效地转导所关注的细胞、组织和/或器官和/或用于基因疗法的用途。
在其他方面，本发明提供了一种用于与本文描述的方法和组合物一起使用的试剂盒。
附图说明
图1是示出将硫酸铜添加到产生用Bba41衣壳假型化的rAAV颗粒的Sf9昆虫细胞培养物的剂量依赖性影响的图。将Sf9昆虫细胞培养物用0微摩尔(μM)、5μM和50μM浓度的硫酸铜进行培养并用具有用于Bba41衣壳产生的载体的重组杆状病毒(rBV)进行感染。在感染后的预定时间之后，从昆虫细胞培养物中分离Bba41衣壳，并且评估Bba41衣壳的感染性。图1示出了用Bba41衣壳假型化的rAAV颗粒的增加的转导活性，该颗粒是在补充有增加浓度的硫酸铜的Sf9昆虫细胞中产生的。
图2是示出将硫酸铜添加到产生用Bba41衣壳假型化的rAAV颗粒的Sf9昆虫细胞培养物的剂量依赖性影响的十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)。图2示出了来自在硫酸铜补充的Sf9昆虫细胞中产生的Bba41衣壳的VP1，该昆虫细胞具有与来自在人胚胎肾293(HEK293)细胞中产生的Bba41衣壳的VP1类似的带型。将HEK293培养物用0μM、5μM和50μM浓度的硫酸铜进行培养并用用于Bba41衣壳产生的质粒进行转染。在转染后的预定时间之后，从HEK293培养物中分离Bba41衣壳。
图3是示出将硫酸铜添加到产生用Bba41衣壳假型化的rAAV颗粒的Sf9昆虫细胞和HEK293细胞培养物的剂量依赖性影响的图。昆虫细胞培养物是摇瓶产生，并且HEK293培养物是摇瓶和生物反应器产生。从细胞培养物中分离Bba41衣壳，并且体外评估Bba41衣壳的感染性。图3示出了用Bba41衣壳假型化的rAAV颗粒的增加的转导活性，该颗粒是在补充有增加浓度的硫酸铜的Sf9昆虫细胞中产生的。增加的转导活性与HEK293细胞中产生的用Bba41衣壳假型化的rAAV颗粒相当。
图4是示出来自在Sf9昆虫细胞和HEK293细胞中产生的rAAV的AAV载体基因组的琼脂糖凝胶(agarose gel)。
图5是示出在Sf9细胞中产生的rAAV衣壳的VP1浓度的图。将昆虫细胞的摇瓶培养物用0μM、25μM和45μM浓度的硫酸铜进行培养并用具有用于AAV5衣壳产生的载体的rBV进行感染。在感染后的预定时间之后，从昆虫细胞培养物中分离AAV5衣壳，并且对AAV5衣壳的VP1浓度进行定量。不同浓度的硫酸铜增加了所产生的AAV5衣壳的VP1浓度。
图6是示出在Sf9细胞中产生的rAAV衣壳的转导活性的图。将昆虫细胞的摇瓶培养物用0μM和25μM浓度的硫酸铜进行培养并用具有用于AAV5衣壳产生的载体的rBV进行感染。在感染后的预定时间之后，从昆虫细胞培养物中分离AAV5衣壳，并且体外评估AAV5衣壳的感染性。25μM硫酸铜提高了所产生的AAV5衣壳的感染性。
图7是示出在有或没有铜的情况下培养的Sf9细胞中产生的rAAV衣壳的VP1浓度的图。将昆虫细胞的生物反应器培养物用0μM和30μM浓度的硫酸铜进行培养并用具有用于AAV5衣壳产生的载体的rBV进行感染。在感染后的预定时间之后，从昆虫细胞培养物中分离AAV5衣壳，并且对AAV5衣壳的VP1浓度进行定量。30μM硫酸铜增加了在生物反应器规模下产生的AAV5衣壳的VP1浓度。
图8是示出用在Sf9细胞中产生的rAAV衣壳感染的细胞中的转基因表达的图。将昆虫细胞的生物反应器培养物用0μM和30μM浓度的硫酸铜进行培养并用具有用于AAV5衣壳产生的载体的重组杆状病毒(rBV)进行感染。在感染后的预定时间之后，从昆虫细胞培养物中分离AAV5衣壳，并且体外评估AAV5衣壳的感染性。30μM硫酸铜提高了在生物反应器规模下产生的AAV5衣壳的感染性。
图9是示出在Sf9细胞中产生的rAAV衣壳的VP1浓度的图。将昆虫细胞的2000升(L)和3L生物反应器培养物和摇瓶培养物用0μM和30μM浓度的硫酸铜进行培养并用具有用于AAV5衣壳产生的载体的重组杆状病毒(rBV)进行感染。在感染后的预定时间之后，从昆虫细胞培养物中分离AAV5衣壳，并且对AAV5衣壳的VP1浓度进行定量。将在铜补充的生物反应器和摇瓶培养物中产生的AAV5衣壳的VP1浓度与在没有铜的摇瓶培养物中产生的AAV5衣壳的VP1浓度进行比较。30μM硫酸铜增加了使用不同产生类型和规模产生的AAV5衣壳的VP1浓度。
图10是示出在Sf9细胞中产生的rAAV衣壳的VP1浓度的图。将昆虫细胞的100毫升(mL)摇瓶培养物用0μM和30μM浓度的硫酸铜进行培养并用具有用于AAV9衣壳产生的载体的rBV进行感染。在感染后的预定时间之后，从昆虫细胞培养物中分离AAV9衣壳，并且对AAV9衣壳的VP1浓度进行定量。30μM硫酸铜增加了所产生的AAV9衣壳的VP1浓度。
图11是示出在Sf9细胞中产生的rAAV衣壳的转导活性的图。将昆虫细胞的摇瓶培养物用0μM、10μM、20μM和30μM浓度的硫酸铜进行培养并用具有用于在不同感染复数(MOI)下进行AAV9衣壳产生的载体的rBV进行感染。在感染后的预定时间之后，从昆虫细胞培养物中分离AAV9衣壳，并且体外评估AAV9衣壳的感染性。不同MOI下不同浓度的硫酸铜提高了所产生的AAV9衣壳的感染性。
具体实施方式
根据需要，本文公开了本公开的详细方面和实施例；然而，应当理解，所公开的方面和实施例仅仅是示例性的并且可以以各种和替代性形式来体现。
除了在实例中或另外明确指示的情况外，本说明书中指示材料量或反应和/或使用条件的所有数值量应理解为由词语“约”修饰。例如，指代“约X”的描述包括对“X”的描述。在一个实例中，术语“约”被理解为在本领域的正常公差范围内，例如在平均值的2个标准偏差内。在不同实例中，“约”是指±0.0001％、±0.0005％、±0.001％、±0.005％、±0.01％、±0.05％、±0.1％、±0.5％、±1％、±5％或±10％的可变性。在进一步实例中，“约”可以理解为在±9％、±8％、±7％、±6％、±5％、±4％、±3％或±2％内。
除非上下文另外清楚地说明，否则本文提供的所有数值均由术语约修饰。所有范围都包括范围的端点。首字母缩略词或其他缩写的第一定义适用于本文中相同缩写的所有后续使用并且加上必要的变更适用于最初定义的缩写的正常语法变体；而且，除非明确相反地说明，否则特性的测量是通过与之前或之后针对相同特性引用的相同技术来确定的。
除非另外指示，否则本文所使用的所有技术和科学术语具有与本公开所属领域的普通技术人员通常理解的含义相同的含义。
还应当理解，本公开不限于下文描述的具体方面和实施例，因为具体组件和/或条件当然可以变化。此外，本文所使用的术语仅用于描述特定方面和实施例并且不旨在以任何方式进行限制。
贯穿本申请，在引用出版物的情况下，这些出版物的公开内容特此通过引用整体并入本申请中，以更全面地描述本发明所属领域的现状。
还应注意的是，除非上下文清楚地另外指示，否则如说明书和所附权利要求中所使用的，单数形式“一”、“一个”和“该”包括复数指代物。例如，以单数形式引用组分旨在包括多种组分。
术语“或”和“和”可以可互换地使用并且可以被理解为意指“和/或”。
术语“包括”与“包含”、“具有”、“含有”或“以……表征”同义。这些术语是包容性和开放式的并且不排除另外的、未叙述的元素或方法步骤。
短语“由……组成”排除权利要求中未指定的任何元素、步骤或成分。当该短语出现在权利要求的主体的条款中，而不是紧跟在序言之后时，其仅限制该条款中所阐述的元素；其他要素作为一个整体不被排除在权利要求之外。
短语“基本上由……组成”将权利要求的范围限制为指定的材料或步骤，加上不会实质上影响所要求保护的主题的基本和新颖特征的那些材料或步骤。
可以替代性地使用术语“包括”、“由……组成”和“基本上由……组成”。当使用这三个术语中的一个时，当前公开和要求保护的主题可以包括使用其他两个术语中的任一个。
根据本发明，发明人已经惊奇地发现，通过在包含有效量的过渡金属或盐的培养基中培养能够产生rAAV的宿主细胞，提高了由宿主细胞产生的rAAV颗粒的感染性。具体地，发明人已经发现，培养基中盐的有效量增加掺入衣壳中的至少VP1蛋白的量，由此增强了所产生的AAV病毒颗粒的感染性。因此，发明人已经发现了产生治疗有效的rAAV的方法和过程。
尽管本文描述的方法可以被公开和描述为步骤，但是应当理解，方法不一定受步骤的顺序限制，因为根据这些方法，一些步骤可以以不同的顺序进行，和/或与本文描述的和/或本领域已知的其他步骤同时进行。
腺相关病毒
一方面，公开了一种通过任何方面或实施例的方法产生的rAAV衣壳。rAAV衣壳具有大于在相同条件下但缺乏有效量的过渡金属的情况下产生的rAAV衣壳的VP1、VP2或VP3蛋白浓度的VP1、VP2或VP3蛋白浓度。
一方面，公开了一种通过任何方面或实施例的任何方法产生的rAAV衣壳。rAAV衣壳表现出增强的感染性。
rAAV和rAAV衣壳包含已知方法中公开的或可以根据已知方法制成的rAAV颗粒，例如US 9,504,762、WO 2018/022608、WO 2019/222136和US2019/0376081中教导的，其公开内容特此通过引用整体并入全文。
“AAV”是腺相关病毒的标准缩写。腺相关病毒是具有由衣壳包覆的基因组的单链DNA细小病毒。当前已经表征了十三种AAV血清型。AAV的一般信息和评论可以在例如以下中找到：Carter，1989，Handbook ofParvoviruses，Vol.1，pp.169-228；以及Berns，1990，Virology，pp.1743-1764，Raven Press，(New York)。然而，完全预期的是，这些相同的原理将适用于另外的AAV血清型，因为众所周知的是，各种血清型在结构和功能两者上均紧密相关，即使在基因水平下也是如此。(参见例如，Blacklowe，1988，Parvoviruses and HumanDisease中的pp.165-174，J.R.Pattison，ed.；以及Rose，Comprehensive Virology 3:1-61(1974))。例如，所有AAV血清型明显地表现出由同源rep基因介导的非常类似的复制特性；并且全部带有三种相关的衣壳蛋白。相关性程度进一步由异源双链体分析来表明，该分析显示血清型之间沿基因组长度有广泛的交叉杂交；并且在末端存在对应于反向末端重复序列(ITR)的相似自退火片段。类似的感染性模式还表明每种血清型的复制功能处于类似的调节控制下。
如本文所使用的，“AAV病毒颗粒”是指由至少一种AAV衣壳蛋白以及衣壳化AAV基因组构成的感染性病毒颗粒。“重组AAV”或“rAAV”、“rAAV病毒粒子”或“rAAV病毒颗粒”或“rAAV载体颗粒”或“AAV病毒”如本文所述是指由至少一种衣壳或Cap蛋白以及衣壳化rAAV载体基因组(vg)构成的病毒颗粒。如果颗粒包含异源多核苷酸(即，除野生型AAV基因组之外的多核苷酸，诸如待递送到哺乳动物细胞的转基因)，则其通常被称为“rAAV载体颗粒”或简称为“rAAV载体”。因此，AAV载体颗粒的产生必然包括rAAV载体的产生，因为此类载体包含在rAAV载体颗粒内。
如本文所使用的，术语“异源基因”、“异源序列”、“异源”、“异源调节序列”、“异源转基因”或“转基因”意味着，所提及的基因或调节序列不天然存在于AAV载体或颗粒中并且已经人工引入其中。例如，这些术语是指包含异源基因和与异源基因可操作地连接的异源调节序列两者的核酸，该异源调节序列控制该基因在宿主细胞中的表达。预期本文的转基因可以编码生物分子(例如，治疗性生物分子)，诸如蛋白质(例如，酶)、多肽、肽、RNA(例如，tRNA、dsRNA、核糖体RNA、催化RNA、siRNA、miRNA、pre-miRNA、lncRNA、snoRNA、小发夹RNA、反式剪接RNA和反义RNA)、基因或碱基编辑系统例如CRISPR基因编辑系统的一个或多个组分、反义寡核苷酸(AON)、反义寡核苷酸(AON)介导的外显子跳跃、触发无义介导的衰变(NMD)的一个或多个毒性外显子、或显性失活突变体。
术语“重组”是指通过使用重组DNA技术来形成的核酸分子或蛋白质。例如，重组核酸分子可以通过组合核酸序列和序列元件来形成。重组蛋白可以是由已经接受重组核酸分子的细胞产生的蛋白。
术语“编码(encodes)”、“经编码(encoded)”和“编码(encoding)”是指用于充当用于在生物过程中合成其他聚合物和大分子的模板的核酸分子诸如基因、互补DNA(cDNA)或信使RNA(mRNA)中特异性核苷酸序列的固有特性。因此，如果由基因产生的mRNA的转录和翻译在细胞或其他生物系统中产生蛋白质，则该基因编码蛋白质。基因或cDNA的编码链(其核苷酸序列与mRNA序列相同并且通常在序列表中提供)和非编码链(用作转录模板)均可以被称为编码该基因或cDNA的蛋白质或其他产物。
“衣壳”是指包装rAAV载体基因组的结构。衣壳包含VP1蛋白或VP3蛋白，但更典型的是包含VP1蛋白、VP2蛋白和VP3蛋白中的所有三种，如在天然AAV中发现的。衣壳蛋白的序列确定rAAV病毒粒子的血清型。rAAV病毒粒子包括源自多个AAV血清型的病毒粒子，包括AAV1、AAV2、AAV4、AAV5、AAV6、AAV7、AAV8、AAV9、AAV10、AAV11、AAV12、AAV13、AAV-rh.10(AAVrh10)、AAV-DJ(AAVDJ)、AAV-DJ8(AAVDJ8)、AAV-1、AAV-2、AAV-2G9、AAV-3、AAV3a、AAV3b、AAV3-3、AAV4、AAV4-4、AAV-5、AAV-6、AAV6.1、AAV6.2、AAV6.1.2、AAV-7、AAV7.2、AAV8、AAV9、AAV9.11、AAV9.13、AAV9.16、AAV9.24、AAV9.45、AAV9.47、AAV9.61、AAV9.68、AAV9.84、AAV9.9、AAV-10、AAV-11、AAV-12、AAV16.3、AAV24.1、AAV27.3、AAV42.12、AAV42-1b、AAV42-2、AAV42-3a、AAV42-3b、AAV42-4、AAV42-5a、AAV42-5b、AAV42-6b、AAV42-8、AAV42-10、AAV42-11、AAV42-12、AAV42-13、AAV42-15、AAV42-aa、AAV43-1、AAV43-12、AAV43-20、AAV43-21、AAV43-23、AAV43-25、AAV43-5、AAV44.1、AAV44.2、AAV44.5、AAV223.1、AAV223.2、AAV223.4、AAV223.5、AAV223.6、AAV223.7、AAV1-7/rh.48、AAV1-8/rh.49、AAV2-15/rh.62、AAV2-3/rh.61、AAV2-4/rh.50、AAV2-5/rh.51、AAV3.1/hu.6、AAV3.1/hu.9、AAV3-9/rh.52、AAV3-11/rh.53、AAV4-8/r11.64、AAV4-9/rh.54、AAV4-19/rh.55、AAV5-3/rh.57、AAV5-22/rh.58、AAV7.3/hu.7、AAV16.8/hu.10、AAV16.12/hu.11、AAV29.3/bb.1、AAV29.5/bb.2、AAV106.1/hu.37、AAV114.3/hu.40、AAV127.2/hu.41、AAV127.5/hu.42、AAV128.3/hu.44、AAV130.4/hu.48、AAV145.1/hu.53、AAV145.5/hu.54、AAV145.6/hu.55、AAV161.10/hu.60、AAV161.6/hu.61、AAV33.12/hu.17、AAV33.4/hu.15、AAV33.8/hu.16、AAV52/hu.19、AAV52.1/hu.20、AAV58.2/hu.25、AAVA3.3、AAVA3.4、AAVA3.5、AAVA3.7、AAVC1、AAVC2、AAVC5、AAVF3、AAVF5、AAVH2、AAVrh.72、AAVhu.8、AAVrh.68、AAVrh.70、AAVpi.1、AAVpi.3、AAVpi.2、AAVrh.60、AAVrh.44、AAVrh.65、AAVrh.55、AAVrh.47、AAVrh.69、AAVrh.45、AAVrh.59、AAVhu.12、AAVH6、AAVLK03、AAVH-1/hu.1、AAVH-5/hu.3、AAVLG-10/rh.40、AAVLG-4/rh.38、AAVLG-9/hu.39、AAVN721-8/rh.43、AAVCh.5、AAVCh.5R1、AAVcy.2、AAVcy.3、AAVcy.4、AAVcy.5、AAVCy.5R1、AAVCy.5R2、AAVCy.5R3、AAVCy.5R4、AAVcy.6、AAVhu.1、AAVhu.2、AAVhu.3、AAVhu.4、AAVhu.5、AAVhu.6、AAVhu.7、AAVhu.9、AAVhu.10、AAVhu.11、AAVhu.13、AAVhu.15、AAVhu.16、AAVhu.17、AAVhu.18、AAVhu.20、AAVhu.21、AAVhu.22、AAVhu.23.2、AAVhu.24、AAVhu.25、AAVhu.27、AAVhu.28、AAVhu.29、AAVhu.29R、AAVhu.31、AAVhu.32、AAVhu.34、AAVhu.35、AAVhu.37、AAVhu.39、AAVhu.40、AAVhu.41、AAVhu.42、AAVhu.43、AAVhu.44、AAVhu.44R1、AAVhu.44R2、AAVhu.44R3、AAVhu.45、AAVhu.46、AAVhu.47、AAVhu.48、AAVhu.48R1、AAVhu.48R2、AAVhu.48R3、AAVhu.49、AAVhu.51、AAVhu.52、AAVhu.54、AAVhu.55、AAVhu.56、AAVhu.57、AAVhu.58、AAVhu.60、AAVhu.61、AAVhu.63、AAVhu.64、AAVhu.66、AAVhu.67、AAVhu.14/9、AAVhu.t 19、AAVrh.2、AAVrh.2R、AAVrh.8、AAVrh.8R、AAVrh.12、AAVrh.13、AAVrh.13R、AAVrh.14、AAVrh.17、AAVrh.18、AAVrh.19、AAVrh.20、AAVrh.21、AAVrh.22、AAVrh.23、AAVrh.24、AAVrh.25、AAVrh.31、AAVrh.32、AAVrh.33、AAVrh.34、AAVrh.35、AAVrh.36、AAVrh.37、AAVrh.37R2、AAVrh.38、AAVrh.39、AAVrh.40、AAVrh.46、AAVrh.48、AAVrh.48.1、AAVrh.48.1.2、AAVrh.48.2、AAVrh.49、AAVrh.51、AAVrh.52、AAVrh.53、AAVrh.54、AAVrh.56、AAVrh.57、AAVrh.58、AAVrh.61、AAVrh.64、AAVrh.64R1、AAVrh.64R2、AAVrh.67、AAVrh.73、AAVrh.74、AAVrh8R、AAVrh8R A586R突变体、AAVrh8R R533A突变体、AAAV、BAAV、山羊AAV、牛AAV、AAVhE1.1、AAVhEr1.5、AAVhER1.14、AAVhEr1.8、AAVhEr1.16、AAVhEr1.18、AAVhEr1.35、AAVhEr1.7、AAVhEr1.36、AAVhEr2.29、AAVhEr2.4、AAVhEr2.16、AAVhEr2.30、AAVhEr2.31、AAVhEr2.36、AAVhER1.23、AAVhEr3.1、AAV2.5T、AAV-PAEC、AAV-LK01、AAV-LK02、AAV-LK03、AAV-LK04、AAV-LK05、AAV-LK06、AAV-LK07、AAV-LK08、AAV-LK09、AAV-LK10、AAV-LK11、AAV-LK12、AAV-LK13、AAV-LK14、AAV-LK15、AAV-LK16、AAV-LK17、AAV-LK18、AAV-LK19、AAV-PAEC2、AAV-PAEC4、AAV-PAEC6、AAV-PAEC7、AAV-PAEC8、AAV-PAEC11、AAV-PAEC12、AAV-2-pre-miRNA-101、AAV-8h、AAV-8b、AAV-h、AAV-b、AAV SM 10-2、AAV改组100-1、AAV改组100-3、AAV改组100-7、AAV改组10-2、AAV改组10-6、AAV改组10-8、AAV改组100-2、AAV SM 10-1、AAV SM 10-8、AAV SM 100-3、AAV SM 100-10、BNP61 AAV、BNP62 AAV、BNP63 AAV、AAVrh.50、AAVrh.43、AAVrh.62、AAVrh.48、AAVhu.19、AAVhu.11、AAVhu.53、AAV4-8/rh.64、AAVLG-9/hu.39、AAV54.5/hu.23、AAV54.2/hu.22、AAV54.7/hu.24、AAV54.1/hu.21、AAV54.4R/hu.27、AAV46.2/hu.28、AAV46.6/hu.29、AAV128.1/hu.43、理想型AAV(ttAAV)、UPENN AAV10或日本AAV10血清型、AAV_po.6、AAV_po.、AAV_po.5、AAV_LK03、AAV_ra.1、AAV_蝙蝠_YNM、AAV_蝙蝠_巴西、AAV_mo.1、AAV_鸟_DA-1、或AAV_小鼠_NY1、Bba21、Bba26、Bba27、Bba29、Bba30、Bba31、Bba32、Bba33、Bba34、Bba35、Bba36、Bba37、Bba38、Bba41、Bba42、Bba43、Bba44、Bce14、Bce15、Bce16、Bce17、Bce18、Bce20、Bce35、Bce36、Bce39、Bce40、Bce41、Bce42、Bce43、Bce44、Bce45、Bce46、Bey20、Bey22、Bey23、Bma42、Bma43、Bpo1、Bpo2、Bpo3、Bpo4、Bpo6、Bpo8、Bpo13、Bpo18、Bpo20、Bpo23、Bpo24、Bpo27、Bpo28、Bpo29、Bpo33、Bpo35、Bpo36、Bpo37、Brh26、Brh27、Brh28、Brh29、Brh30、Brh31、Brh32、Brh33、Bfm17、Bfm18、Bfm20、Bfm21、Bfm24、Bfm25、Bfm27、Bfm32、Bfm33、Bfm34、Bfm35、AAV-rh10、AAV-rh39、AAV-rh43、AAVanc80L65或其任何变体(参见例如美国专利号8,318,480关于非天然混合血清型的公开内容)。国际申请公开号WO 2018/022608和WO 2019/222136中也提供了示例性衣壳，该国际申请整体并入本文。衣壳蛋白还可以是天然VP1、VP2和VP3的变体，包括突变的、嵌合的或改组的蛋白。衣壳蛋白可以是rh.10或AAV的各种进化枝内的其他亚型的蛋白；各种进化枝和亚型例如美国专利号7,906,111中进行了公开。在各种实施例中，AAV病毒颗粒的衣壳具有VP1、VP2或VP3蛋白，其氨基酸序列与来自以下各项的氨基酸序列的一部分至少约50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、96％、97％、98％、99％或100％相同：AAV-1(Genbank登录号AAD27757.1)、AAV-2(NCBI参考序列号YP_680426.1)、AAV-3(NCBI参考序列号NP_043941.1)、AAV-3B(Genbank登录号AAB95452.1)、AAV-4(NCBI参考序列号NP_044927.1)、AAV-5(NCBI参考序列号YP_068409.1)、AAV-6(Genbank登录号AAB95450.1)、AAV-7(NCBI参考序列号YP_077178.1)、AAV-8(NCBI参考序列号YP_077179.1)、AAV-9(Genbank登录号AAS99264.1)、AAV-10(Genbank登录号AAT46337.1)、AAV-11(Genbank登录号AAT46339.1)、AAV-12(Genbank登录号ABI16639.1)、AAV-13(Genbank登录号ABZ10812.1)或WO 2018/022608和WO 2019/222136中公开的任何氨基酸序列。以下中讨论了不同血清型的AAV蛋白的构建和使用：Chao等人，Mol.Ther.2:619-623，2000；Davidson等人，PNAS 97:3428-3432，2000；Xiao等人，J.Virol.72:2224-2232，1998；Halbert等人，J.Virol.74:1524-1532，2000；Halbert等人，J.Virol.75:6615-6624，2001；以及Auricchio等人，Hum.Molec.Genet.10:3075-3081，2001。
在一个实施例中，rAAV颗粒用AAV衣壳假型化，其中VP1蛋白包含SEQ ID NO:2-76中的任何一个的氨基酸序列；或者包含在SEQ ID NO:2-76中的任何一个的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。
AAV病毒颗粒可以是“假型化”或“杂合”AAV病毒颗粒。术语“杂合”和“假型化”如与AAV病毒颗粒相关的术语那样在本文中可互换地使用并且旨在指示Rep蛋白、反向末端重复序列(ITR)和/或衣壳蛋白具有不同的血清型。已经从例如人、狒狒、猪、狨猴、黑猩猩和恒河猴、豚尾猴和/或食蟹猕猴(cynomolgus macaque)中鉴定出大量替代性衣壳变体，例如，如美国专利号9,737,618以及Gao，G.等人，Clades ofAdeno-associated viruses arewidely disseminated in human tissues，J.Virol.，78(12):6381-8(2004)中所公开的，其中的每一个都通过引用整体并入本文。假型化AAV病毒颗粒的产生公开于例如WO 2018/022608和WO 2001/83692中，其中的每一个都通过引用整体并入本文。还考虑了其他类型的AAV病毒颗粒变体，例如具有衣壳突变的AAV病毒颗粒。参见例如Marsic等人，MolecularTherapy，22(11):1900-1909(2014)，其通过引用整体并入本文。例如但不限于，ITR和/或Rep蛋白可以具有例如衣壳蛋白源自在哺乳动物中发现的AAV序列，诸如例如本文中所公开并标示为以下各项的衣壳序列或其变体：Bba21、Bba26、Bba27、Bba29、Bba30、Bba31、Bba32、Bba33、Bba34、Bba35、Bba36、Bba37、Bba38、Bba41、Bba42、Bba43、Bba44、Bce14、Bce15、Bce16、Bce17、Bce18、Bce20、Bce35、Bce36、Bce39、Bce40、Bce41、Bce42、Bce43、Bce44、Bce45、Bce46、Bey20、Bey22、Bey23、Bma42、Bma43、Bpo1、Bpo2、Bpo3、Bpo4、Bpo6、Bpo8、Bpo13、Bpo18、Bpo20、Bpo23、Bpo24、Bpo27、Bpo28、Bpo29、Bpo33、Bpo35、Bpo36、Bpo37、Brh26、Brh27、Brh28、Brh29、Brh30、Brh31、Brh32、Brh33、Bfm17、Bfm18、Bfm20、Bfm21、Bfm24、Bfm25、Bfm27、Bfm32、Bfm33、Bfm34或Bfm35。
如本文所使用的，“AAV载体基因组”、“载体基因组”或“rAAV载体基因组”是指单链核酸。rAAV病毒颗粒具有在衣壳内衣壳化的rAAV载体基因组。rAAV载体基因组具有AAV 5'反向末端重复(ITR)序列和侧接蛋白质编码序列(优选地功能性治疗性蛋白质编码序列；例如FVIII、FIX和PAH)的AAV 3'ITR，其与同AAV病毒基因组异源的转录调节元件可操作地连接，例如，插入在调节元件中或调节元件与蛋白质编码序列之间或蛋白质编码序列的外显子之间的一个或多个启动子和/或增强子和任选地聚腺苷酸化序列和/或一个或多个内含子。rAAV载体基因组是指存在于rAAV病毒颗粒中并且可以是本文公开的核酸序列的有义链或反义链的核酸。此类单链核酸的大小以碱基为单位提供。如本文所使用的，术语“反向末端重复序列”和“ITR”是指在rAAV基因组的5'和3'末端发现的本领域公认的区，其作为病毒DNA复制的起点和作为病毒基因组的包装信号以顺式起作用。AAV ITR与Rep蛋白一起提供从插置在两个侧接ITR之间的核苷酸序列的高效切除和拯救以及该核苷酸序列到宿主细胞基因组中的整合。Yan等人，J.Virol.79(1):364-379(2005)中公开了某些AAV相关ITR的序列。ITR还被发现呈“正位”或“翻转”构型，其中AA'反向重复(形成发夹臂)之间的序列存在于反向补体中(Wilmott，Patrick等人Human gene therapy methods 30.6(2019):206-213)。以下中讨论了不同血清型的AAV载体基因组的构建和使用：Chao等人，Mol.Ther.2:619-623，2000；Davidson等人，PNAS 97:3428-3432，2000；Xiao等人，J.Virol.72:2224-2232，1998；Halbert等人，J.Virol.74:1524-1532，2000；Halbert等人，J.Virol.75:6615-6624，2001；以及Auricchio等人，Hum.Molec.Genet.10:3075-3081，2001。由于广泛的构建体可用性和广泛的表征，下文公开的说明性AAV载体基因组源自血清型2。
术语“治疗有效的AAV”、“治疗有效的AAV颗粒”、“治疗性AAV”、“治疗有效的rAAV”、“治疗有效的rAAV颗粒”、“治疗性rAAV”和“治疗有效的rAAV”是指能够感染细胞使得被感染细胞表达(例如，通过转录和/或通过翻译)所关注的元件(例如，核苷酸序列、蛋白质等)的重组AAV。就此而言，治疗有效的rAAV颗粒可以包括具有带有不同特性的衣壳或载体基因组(vg)的AAV颗粒。例如，治疗有效的rAAV颗粒可以具有带有不同的翻译后修饰的衣壳。在其他实例中，治疗有效的AAV颗粒可以含有带有不同的大小/长度、正链或负链序列、不同的正位/翻转ITR构型正位/翻转、翻转/正位、正位/正位、翻转/翻转等)、不同数量的ITR(1个、2个、3个等)或截短的vg。例如，重叠同源重组在rAAV感染的细胞中在具有5'端截短和3'端截短的核酸之间进行，使得生成编码大蛋白的“完整”核酸，由此重建功能性全长基因。在其他实例中，具有5'端截短和3'端截短的互补核酸序列彼此相互作用，使得在第二链合成期间形成“完整”核酸。“完整”核酸编码大蛋白，由此重建功能性全长基因。治疗有效的rAAV颗粒也被称为重衣壳、完整衣壳或部分完整衣壳。
术语“转导(transduction)”和“转导(transduce)”是指将遗传物质(例如，载体基因组)从rAAV转移到受体细胞中以及在受体细胞中表达来自rAAV遗传物质的转基因。遗传物质的转移是通过感染受体细胞的rAAV颗粒介导的。为此目的，术语“效力”是指被rAAV颗粒感染的一个或多个受体细胞中的转基因表达水平。因此，具有更大效力的rAAV强调，被rAAV感染的受体细胞具有更大的转基因表达。
术语“治疗有效量”意指当施用于患有或易患疾病、病症或病状的受试者时足以治疗、诊断、预防或延迟疾病、病症或病状的症状的发作的治疗剂的量。本领域普通技术人员应当理解，治疗有效量通常经由包括至少一个单位剂量的给药方案施用。术语“治疗有效”是指充分发挥作用使得治疗剂的治疗有效量当施用于患有或易患疾病、病症和/或病状的受试者时足以治疗、诊断、预防和/或延迟疾病、病症和/或病状的症状的发作的、治疗剂的任何元素或组成。例如，如先前指出的，治疗有效的rAAV能够感染细胞，使得被感染细胞表达(例如，通过转录和/或通过翻译)所关注的元件(例如，核苷酸序列、蛋白质等)。治疗有效的rAAV具有载体基因组，被治疗有效的rAAV感染的细胞使用该载体基因组来生成治疗有效的核苷酸序列，被感染细胞使用该治疗有效的核苷酸序列来通过各种方法诸如复制、转录或翻译生成所关注的元件(例如，核苷酸序列、蛋白质等)。还应注意的是，“治疗剂”包括治疗有效的rAAV或治疗性rAAV病毒。
作为实例，“治疗性rAAV病毒”(其是指包含编码治疗性蛋白的异源多核苷酸的rAAV病毒粒子、rAAV病毒颗粒、rAAV载体颗粒或rAAV病毒)可以用于体内替代或补充蛋白。“治疗性蛋白”是具有替代或补偿对应内源蛋白质活性的损失或降低的生物活性的多肽。例如，功能性苯丙氨酸羟化酶(PAH)是苯丙酮尿症(PKU)的治疗性蛋白。因此，例如重组rAAVPAH病毒可以用于治疗患有PKU的受试者的药物。药物可以通过静脉内(IV)施用来施用，并且药物的施用导致受试者的血流中PAH蛋白的表达足以改变受试者的神经递质代谢物或神经递质水平。任选地，药物还可以包含用于预防和/或治疗与rAAV PAH病毒的施用相关联的任何肝毒性的预防性和/或治疗性皮质类固醇。包含预防性或治疗性皮质类固醇治疗的药物可以包含至少5、10、15、20、25、30、35、40、45、50、55、60或更多mg/天的皮质类固醇。包含预防性或治疗性皮质类固醇的药物可以在至少约3、4、5、6、7、8、9、10周或更长的连续时期内施用。PKU疗法还可以任选地包括酪氨酸补充。
掺入到AAV衣壳中的转基因不受限制并且可以是具有治疗性意义的任何异源基因。转基因是与转基因侧翼的载体序列异源的核酸序列，其编码所关注的多肽、蛋白质或其它产物。核酸编码序列以允许宿主细胞中的转基因转录、翻译和/或表达的方式与调节组分操作性地连接。
转基因序列的组成将取决于所得载体的用途。例如，一类转基因序列包括报告序列，其在表达时产生可检测的信号。此类报告序列包括但不限于编码以下各项的DNA序列：b-内酰胺酶；b-半乳糖苷酶(LacZ)；碱性磷酸酶；胸苷激酶；绿色荧光蛋白(GFP)；氯霉素乙酰转移酶(CAT)；荧光素酶；膜结合蛋白，其包括例如CD2、CD4、CD8；流感血凝素蛋白和本领域众所周知的其他蛋白，针对其存在或可以通过常规方法来产生高亲和力抗体；以及包含与来自血凝素或Myc等的抗原标签域适当地融合的膜结合蛋白的融合蛋白。
这些编码序列当与驱动其表达的调节元件相关联时提供可通过常规方法检测的信号，该常规方法包括酶促、放射照相、比色、荧光或其它光谱分析、荧光激活细胞分选分析和免疫分析，包括酶联免疫吸附分析(ELISA)、放射免疫分析(RIA)和免疫组织化学。例如，在标志物序列是LacZ基因的情况下，通过测定β-半乳糖苷酶活性来检测携带信号的载体的存在。在转基因是绿色荧光蛋白或荧光素酶的情况下，携带信号的载体可以通过在发光计中的颜色或光产生来目测测量。
然而，转基因通常是非标记序列，其编码可用于生物学和医学的产物，诸如蛋白质、肽、RNA、酶、显性失活突变体或催化RNA。需要的RNA分子包括tRNA、dsRNA、核糖体RNA、催化性RNA、siRNA、小发夹RNA、反式剪接RNA和反义RNA。有用的RNA序列的一个实例是抑制或消除受治疗动物中靶核酸序列表达的序列。通常，合适的靶序列包括肿瘤靶和病毒性疾病。关于此类靶标的实例，参见下文在与免疫原相关的部分中鉴定的肿瘤学靶标和病毒。
转基因可用于纠正或改善基因缺陷，该基因缺陷可包括正常基因以低于正常水平表达的缺陷或功能基因产物不表达的缺陷。优选类型的转基因序列编码在宿主细胞中表达的治疗性蛋白或多肽。载体可以进一步包含多个转基因，例如以纠正或改善由多亚基蛋白引起的基因缺陷。在某些情况下，不同的转基因可以用于编码蛋白的每个亚基，或编码不同的肽或蛋白。当编码蛋白亚基的DNA的大小较大时，例如对于免疫球蛋白、血小板衍化生长因子或肌营养不良蛋白，这是合乎需要的。为了使细胞产生多亚基蛋白，用含有不同亚基中的每一个的重组病毒感染细胞。替代地，蛋白的不同亚基可以由相同的转基因编码。在这种情况下，单个转基因包括编码亚基中的每一个的DNA，每个亚基的DNA由内部核酶进入位点(IRES)分开。当编码亚基中的每一个的DNA的大小较小时，例如编码亚基和IRES的DNA的总大小小于五千碱基(Kb)时，这是合乎需要的。还应注意的是，由于靶细胞中部分基因组的重组，更长的基因组(即>5(Kb))可能是可行的。作为IRES的替代方案，DNA可以由编码2A肽的序列分离，该2A肽在翻译后事件中自切割。参见例如Donnelly等人，J.Gen.Virol.，78(Pt1):13-21(1997年1月)；Furler，等人，Gene Ther.，8(11):864-873(2001年6月)；Klump等人，Gene Ther.，8(lO):811-817(2001年5月)。该2A肽显著小于IRES，使其在空间是限制因素时非常适合使用。更通常地，当转基因大、由多亚基组成，或共同递送两个转基因时，共同施用携带所需的转基因或亚基的rAAV，以允许它们体内串联以形成单一载体基因组。在此类实施例中，第一AAV可以携带表达单个转基因的表达盒，并且第二AAV可以携带表达不同转基因的表达盒，用于在宿主细胞中共表达。然而，所选择的转基因可以编码任何生物活性产物或其它产物，例如研究所需的产物。
本领域技术人员可以容易地选择合适的转基因。对转基因的选择不认为是对本发明的限制。转基因可以是异源蛋白，并且该异源蛋白可以是治疗性蛋白。示例性的治疗性蛋白包括但不限于：血液因子，诸如b-珠蛋白、血红蛋白、组织纤溶酶原激活剂和凝血因子；集落刺激因子(CSF)；白细胞介素，诸如IL-l、IL-2、IL-3、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9等；生长因子，诸如角质细胞生长因子(KGF)、干细胞生长因子(SCF)、成纤维细胞生长因子(FGF，诸如碱性FGF和酸性FGF)、肝细胞生长因子(HGF)、胰岛素样生长因子(IGF)、骨形态发生蛋白(BMP)、表皮生长因子(EGF)、生长分化因子-9(GDF-9)、肝癌衍生生长因子(HDGF)、肌生长抑制素(GDF-8)、神经生长因子(NGF)、神经营养蛋白、血小板衍生生长因子(PDGF)、血小板生成素(TPO)、转化生长因子α(TGF-a.)、转化生长因子β(TGF-.b.)等；可溶性受体，诸如可溶性TNF-a.受体、可溶性VEGF受体、可溶性白细胞介素受体(例如，可溶性IL-l受体和可溶性II型IL-l受体)、可溶性g/d T细胞受体、可溶性受体的配体结合片段等；酶，诸如a-葡糖苷酶、伊米苷酶(imiglucarase)、b-葡糖脑苷脂酶和阿糖脑苷酶；酶激活剂，诸如组织纤溶酶原激活剂；趋化因子，诸如1P-10、由干扰素-γ诱导的单核因子(Mig)、Groa/IL-8、RANTES、MIP-la、MIR-1b.、MCP-l、PF-4等；血管生成剂，诸如血管内皮生长因子(VEGF，例如VEGF121、VEGF165、VEGF-C、VEGF-2)、神经胶质瘤衍生生长因子、血管生成素、血管生成素-2；等；抗血管生成剂，诸如可溶性VEGF受体；蛋白质疫苗；神经活性肽，诸如神经生长因子(NGF)、缓激肽、胆囊收缩素、胃泌素、促胰液素、催产素、促性腺激素释放激素、β-内啡肽、脑啡肽、P物质、生长抑素、催乳素、甘丙肽、生长激素释放激素、铃蟾肽、强啡肽、华法林、神经降压素、胃动素、促甲状腺素、神经肽Y、黄体生成素、降钙素、胰岛素、胰高血糖素、加压素、血管紧张素II、促甲状腺素释放激素、血管活性肠肽、睡眠肽等；溶栓剂；心房钠尿肽；松弛素；胶质纤维酸性蛋白；卵泡刺激素(FSH)；人α-1抗胰蛋白酶；白血病抑制因子(LIF)；组织因子、黄体生成素；巨噬细胞激活因子；肿瘤坏死因子(TNF)；中性粒细胞趋化因子(NCF)；金属蛋白酶的组织抑制剂；血管活性肠肽；血管生成素；血管紧张素；纤维蛋白；水蛭素；IF-l受体拮抗剂等。所关注的蛋白的一些其他非限制性实例包括：睫状神经营养因子(CNTF)；脑源性神经营养因子(BDNF)；神经营养素3和4/5(NT-3和4/5)；胶质细胞源性神经营养因子(GDNF)；芳香族氨基酸脱羧酶(AADC)；血友病相关凝血蛋白，诸如因子VIII、因子IX、因子X；遗传性血管性水肿相关蛋白，诸如C1抑制剂；肌营养不良蛋白、微小肌营养不良蛋白或微肌营养不良蛋白；溶酶体酸性脂肪酶；苯丙氨酸羟化酶(PAH)；糖原贮积病相关酶，诸如葡萄糖-6-磷酸酶、酸性麦芽糖酶、糖原脱支酶、肌糖原磷酸化酶、肝糖原磷酸化酶、肌磷酸果糖激酶、磷酸化酶激酶(例如，PHKA2)、葡萄糖转运蛋白(例如，GFUT2)、醛缩酶A、b-烯醇酶和糖原合酶；溶酶体酶(例如，β-N-乙酰己糖胺酶A)；及其任何变体。其他转基因包括编码心肌肌球蛋白结合蛋白C、β-肌球蛋白重链、心肌肌钙蛋白T、心肌肌钙蛋白I、肌球蛋白心室必需轻链1、肌球蛋白心室调节轻链2、心脏α肌动蛋白(ACTC)、α-原肌球蛋白、肌联蛋白、四加半LIM蛋白1的转基因以及国际申请公开号WO 2014/170470中的美国专利号中公开的其他转基因。AAV载体还包含常规控制元件或序列，该常规控制元件或序列以允许其在用质粒载体转染或用病毒感染的细胞中转录、翻译和/或表达的方式与转基因可操作地连接。如本文所使用的，“可操作地连接的”序列包括与所关注的基因(GOI)邻接的表达控制序列和以反式或在远处发挥作用以控制所关注的基因的表达控制序列。合适的基因包括以下中讨论的那些基因：Anguela等人“Entering the Modern Era ofGene Therapy”，AnnualRev.ofMed.Vol.70，pages 272-288(2019)；以及Dunbar等人，“Gene Comes ofAge”，Science，Vol.359，Issue 6372，eaan4672(2018)。
表达控制序列包括适当的转录起始、终止、启动子和增强子序列；高效的RNA加工信号，诸如剪接和聚腺苷酸化(polyA)信号；稳定细胞质mRNA的序列；增强翻译效率的序列(例如，Kozak共有序列)；增强蛋白质稳定性的序列；以及当需要时，增强所编码产物的分泌的序列。大量表达控制序列包含(天然的、组成型、诱导型和/或组织特异性启动子)是本领域已知的并且可以被利用。
组成型启动子的实例包括但不限于逆转录病毒劳斯肉瘤病毒(RSV)LTR启动子(任选地具有RSV增强子)、巨细胞病毒(CMV)启动子(任选地具有CMV增强子)(参见例如Boshart等人，Cell，41:521-530(1985))、SV40启动子、二氢叶酸还原酶启动子、b-肌动蛋白启动子、磷酸甘油激酶(PGK)启动子和EF1启动子[英杰公司(Invitrogen)]。诱导型启动子允许调节基因表达并且可以通过外源供应的化合物、环境因素诸如温度、或者特异性生理状态例如急性期、细胞的特定分化状态或仅在复制细胞时的存在来调节。诱导型启动子和诱导型系统可从多种商业来源获得，包括但不限于英杰公司、克罗泰克公司(Clontech)和阿瑞雅德公司(Ariad)。已经描述了许多其他系统，并且本领域技术人员可以容易地选择该系统。通过外源供应的化合物调节的诱导型启动子的实例包括：锌诱导型绵羊金属硫蛋白(MT)启动子、地塞米松(Dex)诱导型小鼠乳腺肿瘤病毒(MMTV)启动子、T7聚合酶启动子系统[WO 98/10088]；蜕皮激素昆虫启动子[No等人，Proc.Natl.Acad.Sci.USA，93:3346-3351(1996)]；四环素抑制型系统[Gossen等人，Proc.Natl.Acad.Sci.USA，89:5547-5551(1992)]；四环素诱导型系统[Gossen等人，Science，268:1766-1769(1995)，还参见Harvey等人，Curr.Opin.Chem.Biol.，2:512-518(1998)]；RU486诱导型系统[Wang等人，Nat.Biotech.，15:239-243(1997)和Wang等人，Gene Ther.，4:432-441(1997)]；以及雷帕霉素诱导型系统[Magari等人，J.Clin.Invest.，100:2865-2872(1997)]。在该上下文中可以有用的其他类型的诱导型启动子是通过特异性生理状态例如温度、急性期、细胞的特定分化状态或仅在复制细胞时调节的启动子。
任选地，可以使用用于转基因的天然启动子。当期望转基因的表达应模仿天然表达时，天然启动子可以是优选的。当转基因的表达必须在时间上或发育上或者以组织特异性方式或者响应于特异性转录刺激来调节时，可以使用天然启动子。在进一步实施例中，还可以使用其他天然表达控制元件诸如增强子元件、聚腺苷酸化位点或Kozak共有序列来模仿天然表达。
转基因还可以包括与组织特异性启动子可操作地连接的基因。例如，如果需要在骨骼肌中表达，应该使用在肌肉中有活性的启动子。这些包括来自编码骨骼b-肌动蛋白、肌球蛋白轻链2A、肌营养不良蛋白、肌肉肌酸激酶的基因的启动子、以及具有比天然存在的启动子更高的活性的合成肌肉启动子(参见Li等人，Nat.Biotech.，17:241-245(1999))。组织特异性启动子的实例已知用于肝脏(白蛋白，Miyatake等人，J.Virol.，71:5124-32(1997)；B型肝炎病毒核心启动子，Sandig等人，Gene Ther.，3:1002-9(1996)；甲胎蛋白(AFP)，Arbuthnot等人，Hum.Gene Ther.，7:1503-14(1996)；骨骨钙蛋白(Stein等人，Mol.Biol.Rep.，24:185-96(1997))；骨唾液蛋白(Chen等人，J.Bone Miner.Res.，11:654-64(1996))；淋巴细胞(CD2，Hansal等人，J.Immunol.，161:1063-8(1998))；免疫球蛋白重链；T细胞受体链)、神经元，诸如神经元特异性烯醇酶(NSE)启动子(Andersen等人，Cell.Mol.Neurobiol.，13:503-15(1993))、神经丝轻链基因(Piccioli等人，Proc.Natl.Acad.Sci.USA，88:5611-5(1991))和神经元特异性vgf基因(Piccioli等人，Neuron，15:373-84(1995))等。
重组AAV可以用于体外(例如，在细胞培养物中)产生所关注的蛋白质。例如，AAV可以在用于体外产生所关注的蛋白质的方法中使用，其中该方法包括提供包含编码异源蛋白的核苷酸序列的重组AAV并使重组AAV与细胞培养物中的细胞接触，由此重组AAV在细胞中表达所关注的蛋白质。编码所关注的蛋白质的核苷酸序列的大小可以变化。例如，核苷酸序列可以为至少约0.1千碱基(kb)、至少约0.2kb、至少约0.3kb、至少约0.4kb、至少约0.5kb、至少约0.6kb、至少约0.7kb、至少约0.8kb、至少约0.9kb、至少约1kb、至少约1.1kb、至少约1.2kb、至少约1.3kb、至少约1.4kb、至少约1.5kb、至少约1.6kb、至少约1.7kb、至少约1.8kb、至少约2.0kb、至少约2.2kb、至少约2.4kb、至少约2.6kb、至少约2.8kb、至少约3.0kb、至少约3.2kb、至少约3.4kb、长度为至少约3.5kb、长度至少为约4.0kb、长度为至少约5.0kb、长度为至少约6.0kb、长度为至少约7.0kb、长度为至少约8.0kb、长度为至少约9.0kb或长度为至少约10.0kb。在一些实施例中，核苷酸的长度为至少约1.4kb。
重组AAV还可以用于体内(例如，在动物诸如哺乳动物中)产生所关注的蛋白质。一些实施例提供用于体内产生所关注的蛋白质的方法，其中该方法包括提供包含编码所关注的蛋白质的核苷酸序列的重组AAV并向受试者施用重组AAV，由此重组AAV在受试者中表达所关注的蛋白质。在一些实施例中，受试者可以是非人哺乳动物，例如猴、狗、猫、小鼠或牛。编码所关注的蛋白质的核苷酸序列的大小可以变化。例如，核苷酸序列可以为至少约0.1kb、至少约0.2kb、至少约0.3kb、至少约0.4kb、至少约0.5kb、至少约0.6kb、至少约0.7kb、至少约0.8kb、至少约0.9kb、至少约1kb、至少约1.1kb、至少约1.2kb、至少约1.3kb、至少约1.4kb、至少约1.5kb、至少约1.6kb、至少约1.7kb、至少约1.8kb、至少约2.0kb、至少约2.2kb、至少约2.4kb、至少约2.6kb、至少约2.8kb、至少约3.0kb、至少约3.2kb、至少约3.4kb、长度为至少约3.5kb、长度至少为约4.0kb、长度为至少约5.0kb、长度为至少约6.0kb、长度为至少约7.0kb、长度为至少约8.0kb、长度为至少约9.0kb或长度为至少约10.0kb。在一些实施例中，核苷酸的长度为至少约1.4kb。
特别令人所关注的是使用重组AAV来表达一种或多种治疗性蛋白以治疗各种疾病或病症。疾病的非限制性实例包括：癌症，诸如癌、肉瘤、白血病、淋巴瘤；以及自身免疫性疾病，诸如多发性硬化症。癌的非限制性实例包括食道癌；肝细胞癌；基底细胞癌、鳞状细胞癌(各种组织)；膀胱癌，包括移行细胞癌；支气管癌；结肠癌；结直肠癌；胃癌；肺癌，包括小细胞肺癌和非小细胞肺癌；肾上腺皮质癌；甲状腺癌；胰腺癌；乳腺癌；卵巢癌；前列腺癌；腺癌；汗腺癌；皮脂腺癌；乳头状癌；乳头状腺癌；囊腺癌；髓样癌；肾细胞癌；导管原位癌或胆管癌；绒毛膜癌；精原细胞瘤；胚胎性癌；威尔姆斯肿瘤；宫颈癌；子宫癌；睾丸癌；成骨癌；上皮癌；以及鼻咽癌。肉瘤的非限制性实例包括纤维肉瘤、粘液肉瘤、脂肪肉瘤、软骨肉瘤、脊索瘤、成骨肉瘤、骨肉瘤、血管肉瘤、内皮肉瘤、淋巴管肉瘤、淋巴管内皮肉瘤、滑膜瘤、间皮瘤、尤因氏肉瘤、平滑肌肉瘤、横纹肌肉瘤和其他软组织肉瘤。实体瘤的非限制性实例包括神经胶质瘤、星形细胞瘤、髓母细胞瘤、颅咽管瘤、室管膜瘤、松果体瘤、血管母细胞瘤、听神经瘤、少突胶质细胞瘤、脑血管瘤、黑色素瘤、神经母细胞瘤和视网膜母细胞瘤。白血病的非限制性实例包括：慢性骨髓增生性综合征；急性骨髓性白血病；慢性淋巴细胞白血病，包括B细胞CLL、T细胞CLL幼淋巴细胞白血病和毛细胞白血病；和急性成淋巴细胞白血病。淋巴瘤的实例包括但不限于：B细胞淋巴瘤，诸如伯基特淋巴瘤；霍奇金淋巴瘤等。
可以使用本文公开的rAAV和方法治疗的疾病的其他非限制性实例包括遗传性病症，包括镰状细胞贫血、囊性纤维化、溶酶体酸性脂肪酶(LAL)缺乏1、泰-萨二氏病(Tay-Sachs disease)、苯丙酮尿症、粘多糖贮积病、糖原贮积病(GSD，例如I、II、III、IV、V、VI、VII、VIII、IX、X、XI、XII、XIII和XIV型GSD)、半乳糖血症、肌营养不良症(例如，杜氏肌营养不良症)、心肌病(例如，肥厚型心肌病、扩张型心肌病、致心律失常性右心室心肌病等)和血友病诸如血友病A(经典血友病)和血友病B(克雷司马斯病)、威尔逊病、法布里病、戈谢病遗传性血管性水肿(HAE)以及α1抗胰蛋白酶缺乏症。另外，本文公开的rAAV和方法可以用于治疗可以通过转基因在肝脏中的局部表达或通过来自肝脏或肝细胞的分泌蛋白的表达来治疗的其他病症。
在受试者(例如，受试者的血清)中表达的异源蛋白的量可以变化。例如，在一些实施例中，蛋白质可以以以下量在受试者的血清中表达：至少约9毫克(mg)/mL、至少约10mg/mL、至少约11mg/mL、至少约12mg/mL、至少约13mg/mL、至少约14mg/mL、至少约15mg/mL、至少约16mg/mL、至少约17mg/mL、至少约18mg/mL、至少约19mg/mL、至少约20mg/mL、至少约21mg/mL、至少约22mg/mL、至少约23mg/mL、至少约24mg/mL、至少约25mg/mL、至少约26mg/mL、至少约27mg/mL、至少约28mg/mL、至少约29mg/mL、至少约30mg/mL，至少约31mg/mL、至少约32mg/mL、至少约33mg/mL、至少约34mg/mL、至少约35mg/mL、至少约36mg/mL、至少约37mg/mL、至少约38mg/mL、至少约39mg/mL、至少约40mg/mL、至少约41mg/mL、至少约42mg/mL、至少约43mg/mL、至少约44mg/mL、至少约45mg/mL、至少约46mg/mL、至少约47mg/mL、至少约48mg/mL、至少约49mg/mL或至少约50mg/mL。所关注的蛋白质可以以以下量表达在受试者的血清中：约9pg/mL、约10pg/mL、约50pg/mL、约100pg/mL、约200pg/mL、约300pg/mL、约400pg/mL、约500pg/mL、约600pg/mL、约700pg/mL、约800pg/mL、约900pg/mL、约1000pg/mL、约1500pg/mL、约2000pg/mL、约2500pg/mL或介于这些值中的任何两个值之间的范围。技术人员将理解，治疗性功效需要所关注的蛋白质的表达水平可以根据非限制性因素(诸如特定的所关注的蛋白质和接受治疗的受试者以及技术人员可以在没有过多实验的情况下使用本领域已知的常规方法容易地确定的蛋白质有效量)而变化。
产生腺相关病毒的方法
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有有效量的过渡金属的培养基中培养的步骤。宿主细胞能够产生rAAV衣壳。过渡金属的有效量增加VP1、VP2或VP3蛋白到rAAV衣壳中的掺入。替代性地，过渡金属的有效量减少VP2或VP3蛋白到rAAV衣壳中的掺入。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有纳摩尔浓度的过渡金属的培养基中培养宿主细胞的步骤。宿主细胞能够产生rAAV衣壳。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有微摩尔浓度的过渡金属的培养基中培养宿主细胞的步骤。宿主细胞能够产生rAAV衣壳。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有有效量的过渡金属的培养基中培养的步骤。宿主细胞能够产生rAAV衣壳。过渡金属的有效量增加VP1和VP3蛋白到rAAV衣壳中的掺入。rAAV衣壳具有大于在相同条件下但缺乏有效量的过渡金属的情况下产生的rAAV衣壳的VP1和VP3蛋白浓度的VP1和VP3蛋白浓度。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有有效量的过渡金属的培养基中培养的步骤。宿主细胞能够产生rAAV衣壳。过渡金属的有效量增加VP1蛋白到rAAV衣壳中的掺入。
一方面，公开了一种用于制备rAAV的方法，并且该方法包括在具有有效量的半胱氨酸蛋白酶抑制剂的培养基中培养的步骤。宿主细胞能够产生rAAV衣壳。半胱氨酸蛋白酶抑制剂的有效量增加VP1、VP2或VP3蛋白到rAAV衣壳中的掺入。替代性地，半胱氨酸蛋白酶抑制剂的有效量减少VP2或VP3蛋白到rAAV衣壳中的掺入。
一方面，本发明提供了一种用于制备rAAV颗粒的方法，该方法包括在包含有效量的盐的培养基中培养能够产生AAV病毒颗粒的宿主细胞的步骤，其中AAV病毒颗粒包含AAV衣壳，该衣壳包含VP1蛋白。盐的有效量增加VP1、VP2或VP3蛋白到rAAV衣壳中的掺入。替代性地，盐的有效量减少VP2或VP3蛋白到rAAV衣壳中的掺入。
在各种实施例中，任何方面或实施例的方法都包括培养具有用于rAAV产生的一种或多种载体的宿主细胞的步骤。在其他实施例中，用于rAAV产生的一种或多种载体包括提供AAV辅助功能的至少一种核酸分子、或提供非AAV辅助功能的至少一种核酸分子、或生成AAV基因组载体的至少一种核酸分子、或其任何组合。任何方面或实施例的方法都进一步包括在允许rAAV产生的条件下培养细胞。该方法任选地包括回收rAAV。例如，可以在共转染之后约12小时、约24小时、约36小时、约48小时、约72小时、约96小时、约120小时或介于这两个时间点中的任何时间点之间的时间收集AAV病毒颗粒。
在一些实施例中，用于产生AAV病毒颗粒的培养物包含以下中的一者或多者：宿主细胞、由野生型或突变腺病毒(诸如温度敏感腺病毒)、疱疹病毒、杆状病毒或者提供辅助功能的质粒构建体提供的合适的辅助病毒功能、AAV rep和cap基因和基因产物、由AAV ITR序列侧接的转基因(诸如诊断性和/或治疗性转基因)、以及支持AAV病毒颗粒产生的合适的培养基和培养基组分。
在一些实施例中，可以用辅助质粒或辅助病毒、病毒构建体和编码AAV cap基因的质粒来转染昆虫或哺乳动物细胞；可以在共转染之后的各个时间点收集rAAV颗粒。
在一些实施例中，在昆虫细胞(例如，Sf9)中产生新型rAAV病毒颗粒。在一些实施例中，通过向宿主细胞提供包含转基因以及Rep和Cap基因的AAV基因组载体来制备AAV病毒颗粒。在一些实施例中，AAV基因组载体包含转基因、AAV Rep基因和AAV Cap基因。在一些实施例中，通过向宿主细胞提供两种或更多种载体来制备rAAV病毒颗粒。例如，在一些实施例中，用包含AAV Rep基因和AAV Cap基因的载体(例如，质粒或杆状病毒)将包含转基因的AAV基因组载体引入(例如，转染或转导)到细胞中。在一些实施例中，用包含转基因的AAV基因组载体、包含AAV Rep基因的载体(例如，质粒或杆状病毒)和包含AAV Cap基因的载体(例如，质粒或杆状病毒)转染或转导的细胞。
在各种实施例中，任何方面或实施例的方法都包括用rBV来感染宿主细胞的步骤。rBV包含编码Rep蛋白的一种或多种核酸分子、编码衣壳蛋白的一种或多种核酸分子和生成AAV基因组载体的至少一种核酸分子。任何方面或实施例的方法都进一步包括在允许rAAV产生的条件下培养细胞。该方法任选地包括回收rAAV。
有多种方法用于生成AAV病毒颗粒：例如但不限于，使用载体和AAV辅助序列进行转染并与AAV辅助病毒(例如，腺病毒、疱疹病毒或牛痘病毒)之一共感染或者用重组AAV载体、AAV辅助载体和附属功能载体进行转染。制成AAV病毒颗粒的方法描述于以下中：例如，美国专利号US6204059、US5756283、US6258595、US6261551、US6270996、US6281010、US6365394、US6475769、US6482634、US6485966、US6943019、US6953690、US7022519、US7238526、US7291498和US7491508、US5064764、US6194191、US6566118、US8137948；或国际公开号WO1996039530、WO1998010088、WO1999014354、WO1999015685、WO1999047691、WO2000055342、WO2000075353、WO2001023597、WO2015191508、WO2019217513、WO2018022608、WO2019222136、WO2020232044、WO2019222132；Methods In MolecularBiology，ed.Richard，Humana Press，NJ(1995)；O'Reilly等人，Baculovirus ExpressionVectors，A Laboratory Manual，Oxford Univ.Press(1994)；Samulski等人，J.Vir.63:3822-8(1989)；Kajigaya等人，Proc.Nat'l.Acad.Sci.USA 88:4646-50(1991)；Ruffing等人，J.Vir.66:6922-30(1992)；Kimbauer等人，Vir.，219:37-44(1996)；Zhao等人，Vir.272:382-93(2000)；其中的每一个的内容通过引用整体并入本文。关于对用于生成AAV病毒颗粒的方法的详细描述，参见例如美国专利号6,001,650、6,004,797和9,504,762，每一个都通过引用整体并入本文。在一个实施例中，使用三重转染方法(参见例如美国专利号6,001,650，其通过引用整体并入本文)来产生AAV病毒颗粒。该方法不需要使用感染性辅助病毒，从而使得能够在不存在任何可检测的辅助病毒的情况下产生AAV病毒颗粒。这是通过使用用于AAV病毒颗粒产生的三种载体即AAV辅助功能载体、附属功能载体和AAV病毒颗粒表达载体来完成的。然而，本领域技术人员将理解，由这些载体编码的核酸序列可以以各种组合提供在两个或更多个载体上。在其他实施例中，可以用辅助质粒或辅助病毒、病毒构建体和编码AAV cap基因的质粒来转染宿主细胞；并且可以在共转染之后的各个时间点收集AAV病毒颗粒。
例如，野生型AAV和辅助病毒可以用于提供必要的复制功能以用于产生AAV病毒颗粒(参见例如美国专利号5,139,941，其通过引用整体并入本文)。替代性地，含有辅助功能基因的质粒与众所周知的辅助病毒之一的感染组合地可以用作复制功能的来源(参见例如美国专利号5,622,856和美国专利号5,139,941，两者均通过引用整体并入本文)。类似地，含有附属功能基因的质粒可以与野生型AAV的感染组合地使用，以提供必要的复制功能。技术人员还可以采用本文描述的和/或本领域众所周知的其他方法来产生AAV病毒颗粒。
在各种实施例中，任何方面或实施例的培养步骤在以下体积中进行：至少20毫升(mL)、至少50mL、至少100mL、至少500mL、至少1升(L)、至少10L、至少50L、至少100L、至少250L、至少500L、至少1000L、至少1500L、至少2000L或至少2500L。
在实例中，培养步骤可以在一个或多个摇瓶中进行。在各种实施例中，任何方面或实施例的培养步骤在以下体积中进行：20mL、30mL、40mL、50mL、60mL、70mL、80mL、90mL、100mL、200mL、300mL、400mL、500mL、600mL、700mL、800mL、900mL、1L、2L、3L、4L或5L。在其他实施例中，培养步骤的体积是介于上文提供的任何两个体积之间的范围。
在其他实例中，培养步骤可以在一个或多个生物反应器中进行。在各种实施例中，任何方面或实施例的培养步骤在以下体积中进行：1L、2L、3L、4L、5L、6L、7L、8L、9L、10L、11L、12L、13L、14L、15L、16L、17L、18L、19L、20L、21L、22L、23L、24L、25L、26L、27L、28L、29L、30L、31L、32L、33L、34L、35L、36L、37L、38L、39L、40L、41L、42L、43L、44L、45L、46L、47L、48L、49L、50L、51L、52L、53L、54L、55L、56L、57L、58L、59L、60L、61L、62L、63L、64L、65L、66L、67L、68L、69L、70L、71L、72L、73L、74L、75L、76L、77L、78L、79L、80L、81L、82L、83L、84L、85L、86L、87L、88L、89L、90L、91L、92L、93L、94L、95L、96L、97L、98L、99L、100L、110L、120L、130L、140L、150L、160L、170L、180L、190L、200L、210L、220L、230L、240L、250L、260L、270L、280L、290L、300L、310L、320L、330L、340L、350L、360L、370L、380L、390L、400L、410L、420L、430L、440L、450L、460L、470L、480L、490L、500L、510L、520L、530L、540L、550L、560L、570L、580L、590L、600L、610L、620L、630L、640L、650L、660L、670L、680L、690L、700L、710L、720L、730L、740L、750L、760L、770L、780L、790L、800L、810L、820L、830L、840L、850L、860L、870L、880L、890L、900L、910L、920L、930L、940L、950L、960L、970L、980L、990L、1000L、1010L、1020L、1030L、1040L、1050L、1060L、1070L、1080L、1090L、1100L、1110L、1120L、1130L、1140L、1150L、1160L、1170L、1180L、1190L、1200L、1210L、1220L、1230L、1240L、1250L、1260L、1270L、1280L、1290L、1300L、1310L、1320L、1330L、1340L、1350L、1360L、1370L、1380L、1390L、1400L、1410L、1420L、1430L、1440L、1450L、1460L、1470L、1480L、1490L、1500L、1510L、1520L、1530L、1540L、1550L、1560L、1570L、1580L、1590L、1600L、1610L、1620L、1630L、1640L、1650L、1660L、1670L、1680L、1690L、1700L、1710L、1720L、1730L、1740L、1750L、1760L、1770L、1780L、1790L、1800L、1810L、1820L、1830L、1840L、1850L、1860L、1870L、1880L、1890L、1900L、1910L、1920L、1930L、1940L、1950L、1960L、1970L、1980L、1990L、2000L、2010L、2020L、2030L、2040L、2050L、2060L、2070L、2080L、2090L、2100L、2110L、2120L、2130L、2140L、2150L、2160L、2170L、2180L、2190L、2200L、2210L、2220L、2230L、2240L、2250L、2260L、2270L、2280L、2290L、2300L、2310L、2320L、2330L、2340L、2350L、2360L、2370L、2380L、2390L、2400L、2410L、2420L、2430L、2440L、2450L、2460L、2470L、2480L、2490L、2500L、2510L、2520L、2530L、2540L、2550L、2560L、2570L、2580L、2590L、2600L、2610L、2620L、2630L、2640L、2650L、2660L、2670L、2680L、2690L、2700L、2710L、2720L、2730L、2740L、2750L、2760L、2770L、2780L、2790L、2800L、2810L、2820L、2830L、2840L、2850L、2860L、2870L、2880L、2890L、2900L、2910L、2920L、2930L、2940L、2950L、2960L、2970L、2980L、2990L或3000L。在其他实施例中，培养步骤的体积是介于上文提供的任何两个体积之间的范围。
术语“载体”被理解为是指任何遗传元件，诸如质粒、噬菌体、转座子、粘粒、杆粒、微小质粒(例如，缺乏细菌元件的质粒)、狗骨(Doggybone)DNA(例如，最小的闭线构建体)、染色体、病毒、病毒粒子(例如，杆状病毒)等，该遗传元件当与适当的控制元件相关联时能够复制并且可以在细胞之间转移基因序列。如本文所使用的，“昆虫细胞相容性载体”或“载体”是指能够生产性地转化或转染昆虫或昆虫细胞的核酸分子。示例性生物载体包括质粒、线性核酸分子和重组病毒。可以采用任何载体，只要其具有昆虫细胞相容性即可。载体可以整合到昆虫细胞基因组中，但昆虫细胞中载体的存在不需要是永久性的，并且还包括瞬时游离载体。可以通过已知的任何方法(例如，通过细胞的化学处理、电穿孔或感染)来引入载体。杆状病毒载体及其使用方法描述于关于昆虫细胞分子工程化的上文引用的参考文献中。
细胞生成rAAV载体基因组所根据的载体可以含有启动子和启动子下游的限制性位点，以允许插入编码一种或多种所关注的蛋白质的多核苷酸，其中启动子和限制性位点位于5'AAV ITR下游和3'AAV ITR上游。载体还可以含有在限制性位点下游和3'AAV ITR上游的转录后调节元件。病毒构建体可以进一步包含在限制性位点处插入并与启动子可操作地连接的多核苷酸，其中多核苷酸包含所关注的蛋白质的编码区。在一些实施例中，病毒构建体进一步包含启动子和启动子下游的限制性位点，以允许插入编码一种或多种所关注的蛋白质的多核苷酸，其中启动子和限制性位点位于5'AAV ITR下游和3'AAV ITR上游。在一些实施例中，病毒构建体进一步包含在限制性位点下游和3'AAV ITR上游的转录后调节元件。在一些实施例中，病毒构建体进一步包含在限制性位点处插入并与启动子可操作地连接的多核苷酸，其中多核苷酸包含所关注的蛋白质的编码区。如技术人员将理解的，本申请中公开的AAV载体中的任何一个可以在该方法中作为病毒构建体用于产生rAAV病毒粒子。
术语“AAV辅助”是指AAV衍生的编码序列，该编码序列可以被表达成提供AAV基因产物，该基因产物进而以反式起作用以用于生产性AAV复制。因此，AAV辅助功能包括主要的AAV开放阅读框(ORF)中的两者rep和cap。Rep表达产物已经被示出为具有许多功能，包括：对AAV DNA复制起点的识别、结合和切刻；DNA解旋酶活性；以及对来自AAV(或其他异源)启动子的转录的调制等。衣壳(Cap)表达产物供应必要的包装功能。本文使用AAV辅助功能来以反式补充AAV载体基因组中缺失的AAV功能。
对于产生，具有AAV辅助功能的细胞产生足以形成衣壳的重组衣壳蛋白。该衣壳至少包含VP1蛋白和VP3蛋白，但更典型的是包含VP1蛋白、VP2蛋白和VP3蛋白中的所有三种，如在天然AAV中发现的。衣壳蛋白的序列确定由宿主细胞产生的AAV病毒粒子的血清型。可用于本发明的衣壳包括源自多种AAV血清型的衣壳，包括1、2、3、3B、4、5、6、7、8、9、10、11、12、13或混合血清型(参见例如美国专利号8318480关于非天然混合血清型的公开内容)。衣壳蛋白还可以是天然VP1、VP2和VP3的变体，包括突变的、嵌合的或改组的蛋白。衣壳蛋白可以是rh.10或AAV的各种进化枝内的其他亚型的蛋白；各种进化枝和亚型例如美国专利号7,906,111中进行了公开。由于广泛构建体的可用性和广泛的表征，下文公开的说明性AAV载体源自血清型2。以下中讨论了不同血清型的AAV载体和AAV蛋白的构建和使用：Chao等人，Mol.Ther.2:619-623，2000；Davidson等人，PNAS 97:3428-3432，2000；Xiao等人，J.Virol.72:2224-2232，1998；Halbert等人，J.Virol.74:1524-1532，2000；Halbert等人，J.Virol.75:6615-6624，2001；以及Auricchio等人，Hum.Molec.Genet.10:3075-3081，2001。
在各种实施例中，编码VP蛋白的核苷酸序列可以与合适的表达控制序列可操作地连接。在各种实施例中，编码Rep蛋白的核苷酸序列可以与合适的表达控制序列诸如真核启动子可操作地连接。例如，核苷酸序列可以与真核启动子可操作地连接。在另一个实例中，核苷酸序列可以与杆状病毒启动子诸如多角体蛋白(Polh)启动子、ΔIE1启动子、p5启动子、p10启动子、p40启动子、金属硫蛋白启动子、39K启动子、p6.9启动子和orf46启动子可操作地连接。
对于产生，具有AAV辅助功能的细胞产生Rep蛋白，以促进rAAV的产生。已经发现，当细胞中表达至少一种大Rep蛋白(Rep78或Rep68)和至少一种小Rep蛋白(Rep52和Rep40)时，可以产生感染性颗粒。在具体实施例中，表达Rep78、Rep68、Rep52和Rep40中的所有四个。替代性地，表达Rep78和Rep52、Rep78和Rep40、Rep68和Rep52或Rep68和Rep40。下文的实例展现了Rep78/Rep52组合的使用。Rep蛋白可以源自AAV-2或其他血清型。在各种实施例中，编码Rep蛋白的核苷酸序列可以与合适的表达控制序列可操作地连接。在各种实施例中，编码Rep蛋白的核苷酸序列可以与合适的表达控制序列诸如真核启动子可操作地连接。例如，核苷酸序列可以与真核启动子可操作地连接。在其他实例中，核苷酸序列可以与杆状病毒启动子诸如多角体蛋白(Polh)启动子、ΔIE1启动子、p5启动子、p10启动子、p40启动子、金属硫蛋白启动子、39K启动子、p6.9启动子和orf46启动子可操作地连接。
在一些实施例中，AAV cap基因存在于质粒或杆粒中。质粒可以进一步包括AAVrep基因，该基因可以对应于或不对应于与cap基因相同的血清型。来自任何AAV血清型的cap基因和/或rep基因。
具有AAV辅助功能的细胞还可以产生有助于组装衣壳的组装激活蛋白(AAP)。在各种实施例中，编码AAP的核苷酸序列可以与合适的表达控制序列可操作地连接。例如，核苷酸序列可以与真核启动子可操作地连接。在其他实例中，核苷酸序列可以与杆状病毒启动子诸如多角体蛋白(Polh)启动子、ΔIE1启动子、p5启动子、p10启动子、p40启动子、金属硫蛋白启动子、39K启动子、p6.9启动子和orf46启动子可操作地连接。
术语“非AAV辅助功能”是指AAV复制所依赖的非AAV衍生的病毒和/或细胞功能。因此，该术语捕获了AAV复制所需的蛋白质和RNA，包括参与AAV基因转录的激活、阶段特异性AAV mRNA剪接、AAV DNA复制、Cap表达产物的合成和AAV衣壳组装的那些部分。基于病毒的附属功能可以源自任何已知的辅助病毒，诸如腺病毒、疱疹病毒(除了单纯疱疹病毒1型)和牛痘病毒。
术语“非AAV辅助功能载体”通常是指包含提供附属功能的核苷酸序列的核酸分子。附属功能载体可以转染到合适的宿主细胞中，其中载体然后能够支持宿主细胞中的AAV病毒粒子产生。该术语明确排除自然界中存在的感染性病毒颗粒，诸如腺病毒、疱疹病毒或牛痘病毒颗粒。因此，附属功能载体可以是质粒、噬菌体、转座子或粘粒的形式。具体地，已经证明，附属辅助功能不需要腺病毒基因的完整补体。例如，不能够进行DNA复制和晚期基因合成的腺病毒突变体已经被示出为允许AAV复制。Ito等人，(1970)J.Gen.Virol.9:243；Ishibashi等人，(1971)Virology 45:317。类似地，E2B区和E3区内的突变体已经被示出为支持AAV复制，从而表明E2B区和E3区很可能不参与提供附属功能。Carter等人，(1983)Virology 126:505。然而，E1区有缺陷或具有缺失的E4区的腺病毒不能够支持AAV复制。因此，E1A区和E4区可能是AAV复制直接或间接所需的。Laughlin等人，(1982).J.Virol.41:868；Janik等人，(1981)Proc.Natl.Acad.Sci.USA78:1925；Carter等人，(1983)Virology126:505。其他表征的Ad突变体包括：E1B(Laughlin等人(1982)，supra；Janik等人(1981)，supra；Ostrove等人，(1980)Virology 104:502)；E2A(Handa等人，(1975)J.Gen.Virol.29:239；Strauss等人，(1976)J.Virol.17:140；Myers等人，(1980)J.Virol.35:665；Jay等人，(1981)Proc.Natl.Acad.Sci.USA 78:2927；Myers等人，(1981)J.Biol.Chem.256:567)；E2B(Carter，Adeno-Associated Virus Helper Functions，I CRC Handbook ofParvoviruses(P.Tijssen ed.，1990)中)；E3(Carter等人(1983)，supra)；以及E4(Carter等人(1983)，supra；Carter(1995))。尽管对在E1B编码区中具有突变的腺病毒所提供的附属功能的研究已经产生了矛盾的结果，Samulski等人，(1988)J.Virol.62:206-210最近报道了E1B55k是AAV病毒粒子产生所需的，而E1B19k不是。另外，国际公开WO 97/17458和Matshushita等人，(1998)Gene Therapy5:938-945描述了编码各种Ad基因的附属功能载体。特别优选的附属功能载体包含腺病毒VA RNA编码区、腺病毒E4 ORF6编码区、腺病毒E2A72kD编码区、腺病毒E1A编码区和缺乏完整E1B55k编码区的腺病毒E1B区。此类载体描述于国际公开号WO 01/83797中。
用于表达异源蛋白的昆虫细胞的用途被充分证明，将核酸诸如载体例如昆虫细胞相容性载体引入到此类细胞中的方法和将此类细胞维持在培养物中的方法也是如此。(参见例如METHODS IN MOLECULAR BIOLOGY，ed.Richard，Humana Press，N J(1995)；O'Reilly等人，BACULOVIRUS EXPRESSION VECTORS，A LABORATORY MANUAL，Oxford Univ.Press(1994)；Samulski等人，J.Vir.(1989)vol.63，pp.3822-3828；Kajigaya等人，Proc.Nat'l.Acad.Sci.USA(1991)vol.88，pp.4646-4650；Ruffing等人，J.Vir.(1992)vol.66，pp.6922-6930；Kirnbauer等人，Vir.(1996)vol.219，pp.37-44；Zhao等人，Vir.(2000)vol.272，pp.382-393；以及美国专利号6,204,059)。在一些实施例中，编码昆虫细胞中的AAV的核酸构建体是昆虫细胞相容性载体。“表达载体”是指包含重组多核苷酸的载体，该重组多核苷酸包含与待表达的核苷酸序列操作性地连接的表达控制序列。表达载体包含足够的顺式作用元件以用于表达；用于表达的其他元件可以由宿主细胞或体外表达系统供应。表达载体包括本领域已知的所有表达载体，诸如粘粒、质粒(例如，裸露的或包含在脂质体中)、人工染色体和掺入了重组多核苷酸的病毒。如本文所使用的，“昆虫细胞相容性载体”或“载体”是指能够生产性地转化或转染昆虫或昆虫细胞的核酸分子。示例性生物载体包括质粒、线性核酸分子和重组病毒。可以采用任何载体，只要其具有昆虫细胞相容性即可。载体可以整合到昆虫细胞基因组中，但昆虫细胞中载体的存在不需要是永久性的，并且还包括瞬时游离载体。可以通过已知的任何方法(例如，通过细胞的化学处理、电穿孔或感染)来引入载体。在一些实施例中，载体是杆状病毒、病毒载体或质粒。在更优选的实施例中，载体是杆状病毒，即构建体是杆状病毒载体。杆状病毒载体及其使用方法描述于关于昆虫细胞分子工程化的上文引用的参考文献中。
杆状病毒穿梭载体或杆粒用于生成杆状病毒。杆粒作为大质粒在细菌诸如大肠杆菌中繁殖。当转染到昆虫细胞中时，杆粒生成杆状病毒。
在一些实施例中，培养基是感染或转染培养基(例如，其中用基因来感染或转染产生AAV病毒颗粒的宿主细胞的培养基(感染或转染培养基)。
在另一个实施例中，培养基是产生者培养基(例如，宿主细胞在其中产生AAV病毒颗粒的培养基)。这些培养基包括但不限于由生命技术公司(Life Technologies)生产的培养基，包括改良的伊格尔培养基(MEM)、杜氏改良的伊格尔培养基(DMEM)、定制调配物诸如美国专利号6,566,118中描述的调配物和如美国专利号6,723,551中描述的Sf-900 II SFM培养基，其中的每一个都通过引用整体并入本文，特别是关于用于产生AAV病毒颗粒的定制培养基调配物。可以使用的细胞培养基的其他实例包括Sf-900 III SFM(生命技术公司)、ExpiSf CD Medium(生命技术公司)、Express Five SFM(生命技术公司)、IS SfInsect(富士胶片株式会社(Fujifilm))、TheraPEAK(龙沙公司(Lonza))和CD昆虫细胞培养基(密理博西格玛公司(Millipore Sigma))。
还可以使用各种实施例中公开的方法来产生rAAV颗粒。在一些情况下，可以通过使用稳定地表达rAAV颗粒产生所必需的一些组分的昆虫或哺乳动物细胞来产生rAAV颗粒。例如，可以将包含AAV rep和cap基因以及可选择的标志物诸如新霉素抗性基因的质粒(或多个质粒)整合到细胞的基因组中。在另一个实例中，可以将包含可选择的标志物诸如新霉素抗性基因的质粒(或多个质粒)整合到细胞的基因组中。昆虫、真菌或哺乳动物细胞然后可以与辅助病毒(例如，提供辅助功能的腺病毒或杆状病毒)以及包含5'和3'AAV ITR(以及编码异源蛋白的核苷酸序列，如果需要的话)的病毒载体共感染。该方法的优点是细胞是可选择的并且适合rAAV的大规模产生。作为另一个非限制性实例，腺病毒或杆状病毒而不是质粒可以用于将宿主调节基因、rep基因和cap基因引入到包装细胞中。
宿主细胞
宿主细胞可以是允许产生AAV病毒颗粒并且可以在培养物中维持的任何无脊椎动物或脊椎动物细胞类型。
在一个实施例中，宿主细胞是昆虫细胞或哺乳动物细胞。
在另一个实施例中，宿主细胞是昆虫细胞。
在一些实施例中，昆虫细胞来自草地贪夜蛾诸如Sf9、Sf21、Sf900+、果蝇细胞系、蚊子细胞系例如白纹伊蚊衍生的细胞系、家蚕细胞系例如桑蚕细胞系、粉纹夜蛾细胞系诸如High Five细胞、或鳞翅目细胞系诸如暗巫夜蛾细胞系。优选的昆虫细胞是来自对杆状病毒感染敏感的昆虫物种的细胞，包括High Five、Sf9、Se301、SeIZD2109、SeUCRl、Sf900+、Sf21、BTI-TN-5B 1-4、MG-1、Tn368、HzAml、BM-N、Ha2302、Hz2E5和Ao38。
在其他实施例中，宿主细胞是Sf9细胞。
在一个实施例中，宿主细胞是哺乳动物细胞。
在另一个实施例中，哺乳动物细胞是HEK293、HeLa、CHO、NSO、SP2/0、PER.C6、Vera、RD、BHK、HT 1080、A549、Cos-7、ARPE-19或MRC-5细胞。
AAV血清型
已经表征了至少十三种AAV血清型，如表1所示。本发明涵盖但不限于这些特异性AAV血清型。
表1.AAV血清型。
AAV的一般信息和评论可以在例如Carter，1989，Handbook ofParvoviruses，Vol.1，pp.169-228和Berns，1990，Virology，pp.1743-1764，Raven Press，(New York)中找到。然而，完全预期的是，这些相同的原理将适用于另外的AAV血清型，因为众所周知的是，各种血清型在结构和功能两者上均紧密相关，即使在基因水平下也是如此。(参见例如Blacklowe，1988，Parvoviruses and Human Disease中的pp.165-174，J.R.Pattison，ed.；以及Rose，Comprehensive Virology 3:1-61(1974))。例如，所有AAV血清型明显地表现出由同源rep基因介导的非常类似的复制特性；并且全部带有三种相关的衣壳蛋白，诸如在AAV-6中表达的衣壳蛋白。相关性程度进一步由异源双链体分析表明，该分析显示血清型之间沿基因组长度具有广泛的交叉杂交；并且在末端存在对应于ITR的相似自退火片段。类似的感染性模式还表明每种血清型的复制功能处于类似的调节控制下。
AAV“rep”和“cap”基因分别是编码复制和衣壳化蛋白的基因。AAV rep和cap基因已经在检查的所有AAV血清型中发现并且在本文中和引用的参考文献中进行了描述。在野生型AAV中，rep和cap基因通常发现在病毒基因组中彼此相邻(即，它们作为邻接的或重叠的转录单元“偶联”在一起)，并且它们通常在AAV血清型中是保守的。AAV rep和cap基因也单独称为和统称为“AAV包装基因”。根据本发明的AAV cap基因编码Cap蛋白，该蛋白能够在rep和腺辅助功能的存在下包装AAV载体并且能够结合靶细胞受体。在一些实施例中，AAVcap基因编码具有氨基酸序列的衣壳蛋白，该氨基酸序列源自特定AAV血清型例如表1所示的血清型；或源自在哺乳动物例如人、狒狒、猪、狨猴、黑猩猩或猕猴(macaque)(例如，恒河猴(rhesus)(恒河猴(Macaca mulatta))、猕猴(cynomolgus)(“长尾的(long-tailed)”)(长尾猕猴(M.fascicularis))或豚尾猴(pigtailed)(豚尾猴(M.nemestrina)))中发现的AAV替代性衣壳变体序列。
用于产生AAV的AAV序列可以源自任何AAV血清型的基因组。AAV血清型可以具有在氨基酸和核酸水平上显著同源的基因组序列、提供类似的一组遗传功能、产生基本上在物理上和功能上等效的病毒粒子并且通过几乎相同的机制来复制和组装。参见例如GenBank登录号U89790；GenBank登录号J01901；GenBank登录号AF043303；GenBank登录号AF085716；Chlorini等人，J.Vir.71:6823-33(1997)；Srivastava等人，J.Vir.45:555-64(1983)；Chlorini等人，J.Vir.73:1309-1319(1999)；Rutledge等人，J.Vir.72:309-319(1998)；以及Wu等人，J.Vir.74:8635-47(2000)，以上各项关于AAV血清型的基因组序列和/或对基因组相似性的讨论通过引用并入本文。
许多已知AAV血清型的基因组组织可以非常类似。AAV的基因组是长度小于约5,000个核苷酸(nt)的线性单链DNA分子。反向末端重复序列(ITR)侧接非结构复制(Rep)蛋白和结构(VP)蛋白的独特编码核苷酸序列。VP蛋白形成衣壳。末端145nt是自身互补的并且被组织成使得形成T形发夹的能量稳定的分子内双链体可以形成。这些发夹结构作为病毒DNA复制的起点起作用，从而充当用于细胞DNA聚合酶复合物的引物。Rep基因编码Rep蛋白Rep78、Rep68、Rep52和Rep40。Rep78和Rep68由p5启动子转录，并且Rep52和Rep40由p19启动子转录。cap基因编码VP蛋白VP1、VP2和VP3。cap基因由p40启动子转录。
在各种实施例中，提供AAV辅助功能的载体包含编码衣壳蛋白、Rep蛋白或AAP蛋白的一个或多个核苷酸序列。来自任何AAV血清型的cap基因、rep基因和/或AAP基因(包括但不限于AAV1(NCBI参考序列号/Genbank登录号NC_002077.1)、AAV2(NCBI参考序列号/Genbank登录号NC_001401.2)、AAV3(NCBI参考序列号/Genbank登录号NC_001729.1)、AAV3B(NCBI参考序列号/Genbank登录号AF028705.1)、AAV4(NCBI参考序列号/Genbank登录号NC_001829.1)、AAV5(NCBI参考序列号/Genbank登录号NC_006152.1)、AAV6(NCBI参考序列号/Genbank登录号AF028704.1)、AAV7(NCBI参考序列号/Genbank登录号NC_006260.1)、AAV8(NCBI参考序列号/Genbank登录号NC_006261.1)、AAV9(NCBI参考序列号/Genbank登录号AX753250.1)、AAV10(NCBI参考序列号/Genbank登录号AY631965.1)、AAV11(NCBI参考序列号/Genbank登录号AY631966.1)、AAV12(NCBI参考序列号/Genbank登录号DQ813647.1)、AAV13(NCBI参考序列号/Genbank登录号EU285562.1)是AAV-rh.10(AAVrh10)、AAV-DJ(AAVDJ)、AAV-DJ8(AAVDJ8)、AAV-1、AAV-2、AAV-2G9、AAV-3、AAV3a、AAV3b、AAV3-3、AAV4、AAV4-4、AAV-5、AAV-6、AAV6.1、AAV6.2、AAV6.1.2、AAV-7、AAV7.2、AAV8、AAV9、AAV9.11、AAV9.13、AAV9.16、AAV9.24、AAV9.45、AAV9.47、AAV9.61、AAV9.68、AAV9.84、AAV9.9、AAV-10、AAV-11、AAV-12、AAV16.3、AAV24.1、AAV27.3、AAV42.12、AAV42-1b、AAV42-2、AAV42-3a、AAV42-3b、AAV42-4、AAV42-5a、AAV42-5b、AAV42-6b、AAV42-8、AAV42-10、AAV42-11、AAV42-12、AAV42-13、AAV42-15、AAV42-aa、AAV43-1、AAV43-12、AAV43-20、AAV43-21、AAV43-23、AAV43-25、AAV43-5、AAV44.1、AAV44.2、AAV44.5、AAV223.1、AAV223.2、AAV223.4、AAV223.5、AAV223.6、AAV223.7、AAV1-7/rh.48、AAV1-8/rh.49、AAV2-15/rh.62、AAV2-3/rh.61、AAV2-4/rh.50、AAV2-5/rh.51、AAV3.1/hu.6、AAV3.1/hu.9、AAV3-9/rh.52、AAV3-11/rh.53、AAV4-8/r11.64、AAV4-9/rh.54、AAV4-19/rh.55、AAV5-3/rh.57、AAV5-22/rh.58、AAV7.3/hu.7、AAV16.8/hu.10、AAV16.12/hu.11、AAV29.3/bb.1、AAV29.5/bb.2、AAV106.1/hu.37、AAV114.3/hu.40、AAV127.2/hu.41、AAV127.5/hu.42、AAV128.3/hu.44、AAV130.4/hu.48、AAV145.1/hu.53、AAV145.5/hu.54、AAV145.6/hu.55、AAV161.10/hu.60、AAV161.6/hu.61、AAV33.12/hu.17、AAV33.4/hu.15、AAV33.8/hu.16、AAV52/hu.19、AAV52.1/hu.20、AAV58.2/hu.25、AAVA3.3、AAVA3.4、AAVA3.5、AAVA3.7、AAVC1、AAVC2、AAVC5、AAVF3、AAVF5、AAVH2、AAVrh.72、AAVhu.8、AAVrh.68、AAVrh.70、AAVpi.1、AAVpi.3、AAVpi.2、AAVrh.60、AAVrh.44、AAVrh.65、AAVrh.55、AAVrh.47、AAVrh.69、AAVrh.45、AAVrh.59、AAVhu.12、AAVH6、AAVLK03、AAVH-1/hu.1、AAVH-5/hu.3、AAVLG-10/rh.40、AAVLG-4/rh.38、AAVLG-9/hu.39、AAVN721-8/rh.43、AAVCh.5、AAVCh.5R1、AAVcy.2、AAVcy.3、AAVcy.4、AAVcy.5、AAVCy.5R1、AAVCy.5R2、AAVCy.5R3、AAVCy.5R4、AAVcy.6、AAVhu.1、AAVhu.2、AAVhu.3、AAVhu.4、AAVhu.5、AAVhu.6、AAVhu.7、AAVhu.9、AAVhu.10、AAVhu.11、AAVhu.13、AAVhu.15、AAVhu.16、AAVhu.17、AAVhu.18、AAVhu.20、AAVhu.21、AAVhu.22、AAVhu.23.2、AAVhu.24、AAVhu.25、AAVhu.27、AAVhu.28、AAVhu.29、AAVhu.29R、AAVhu.31、AAVhu.32、AAVhu.34、AAVhu.35、AAVhu.37、AAVhu.39、AAVhu.40、AAVhu.41、AAVhu.42、AAVhu.43、AAVhu.44、AAVhu.44R1、AAVhu.44R2、AAVhu.44R3、AAVhu.45、AAVhu.46、AAVhu.47、AAVhu.48、AAVhu.48R1、AAVhu.48R2、AAVhu.48R3、AAVhu.49、AAVhu.51、AAVhu.52、AAVhu.54、AAVhu.55、AAVhu.56、AAVhu.57、AAVhu.58、AAVhu.60、AAVhu.61、AAVhu.63、AAVhu.64、AAVhu.66、AAVhu.67、AAVhu.14/9、AAVhu.t 19、AAVrh.2、AAVrh.2R、AAVrh.8、AAVrh.8R、AAVrh.12、AAVrh.13、AAVrh.13R、AAVrh.14、AAVrh.17、AAVrh.18、AAVrh.19、AAVrh.20、AAVrh.21、AAVrh.22、AAVrh.23、AAVrh.24、AAVrh.25、AAVrh.31、AAVrh.32、AAVrh.33、AAVrh.34、AAVrh.35、AAVrh.36、AAVrh.37、AAVrh.37R2、AAVrh.38、AAVrh.39、AAVrh.40、AAVrh.46、AAVrh.48、AAVrh.48.1、AAVrh.48.1.2、AAVrh.48.2、AAVrh.49、AAVrh.51、AAVrh.52、AAVrh.53、AAVrh.54、AAVrh.56、AAVrh.57、AAVrh.58、AAVrh.61、AAVrh.64、AAVrh.64R1、AAVrh.64R2、AAVrh.67、AAVrh.73、AAVrh.74、AAVrh8R、AAVrh8R A586R突变体、AAVrh8R R533A突变体、AAAV、BAAV、山羊AAV、牛AAV、AAVhE1.1、AAVhEr1.5、AAVhER1.14、AAVhEr1.8、AAVhEr1.16、AAVhEr1.18、AAVhEr1.35、AAVhEr1.7、AAVhEr1.36、AAVhEr2.29、AAVhEr2.4、AAVhEr2.16、AAVhEr2.30、AAVhEr2.31、AAVhEr2.36、AAVhER1.23、AAVhEr3.1、AAV2.5T、AAV-PAEC、AAV-LK01、AAV-LK02、AAV-LK03、AAV-LK04、AAV-LK05、AAV-LK06、AAV-LK07、AAV-LK08、AAV-LK09、AAV-LK10、AAV-LK11、AAV-LK12、AAV-LK13、AAV-LK14、AAV-LK15、AAV-LK16、AAV-LK17、AAV-LK18、AAV-LK19、AAV-PAEC2、AAV-PAEC4、AAV-PAEC6、AAV-PAEC7、AAV-PAEC8、AAV-PAEC11、AAV-PAEC12、AAV-2-pre-miRNA-101、AAV-8h、AAV-8b、AAV-h、AAV-b、AAV SM 10-2、AAV改组100-1、AAV改组100-3、AAV改组100-7、AAV改组10-2、AAV改组10-6、AAV改组10-8、AAV改组100-2、AAV SM10-1、AAV SM 10-8、AAV SM 100-3、AAV SM 100-10、BNP61 AAV、BNP62 AAV、BNP63 AAV、AAVrh.50、AAVrh.43、AAVrh.62、AAVrh.48、AAVhu.19、AAVhu.11、AAVhu.53、AAV4-8/rh.64、AAVLG-9/hu.39、AAV54.5/hu.23、AAV54.2/hu.22、AAV54.7/hu.24、AAV54.1/hu.21、AAV54.4R/hu.27、AAV46.2/hu.28、AAV46.6/hu.29、AAV128.1/hu.43、理想型AAV(ttAAV)、UPENN AAV10或日本AAV10血清型、AAV_po.6、AAV_po.、AAV_po.5、AAV_LK03、AAV_ra.1、AAV_蝙蝠_YNM、AAV_蝙蝠_巴西、AAV_mo.1、AAV_鸟_DA-1、或AAV_小鼠_NY1、Bba21、Bba26、Bba27、Bba29、Bba30、Bba31、Bba32、Bba33、Bba34、Bba35、Bba36、Bba37、Bba38、Bba41、Bba42、Bba43、Bba44、Bce14、Bce15、Bce16、Bce17、Bce18、Bce20、Bce35、Bce36、Bce39、Bce40、Bce41、Bce42、Bce43、Bce44、Bce45、Bce46、Bey20、Bey22、Bey23、Bma42、Bma43、Bpo1、Bpo2、Bpo3、Bpo4、Bpo6、Bpo8、Bpo13、Bpo18、Bpo20、Bpo23、Bpo24、Bpo27、Bpo28、Bpo29、Bpo33、Bpo35、Bpo36、Bpo37、Brh26、Brh27、Brh28、Brh29、Brh30、Brh31、Brh32、Brh33、Bfm17、Bfm18、Bfm20、Bfm21、Bfm24、Bfm25、Bfm27、Bfm32、Bfm33、Bfm34、Bfm35、AAV-rh10、AAV-rh39、AAV-rh43、AAVanc80L65或其任何变体)可以在本文中用于产生重组AAV示例性衣壳还提供在国际申请号WO 2018/022608和WO 2019/222136中，其整体并入本文。上文提供的每个NCBI参考序列号或Genbank登录号也通过引用并入本文。在一些实施例中，AAV cap基因编码来自血清型1、血清型2、血清型3、血清型3B、血清型4、血清型5、血清型6、血清型7、血清型8、血清型9、血清型10、血清型11、血清型12、血清型13或其变体的衣壳。
除了衣壳、Rep和AAP基因之外，实施例还包含表达辅助蛋白的外源多核苷酸。可以以各种组合在宿主细胞中表达的辅助基因产物包括但不限于草地贪夜蛾FKBP46、人FKBP52、腺病毒E1A、E1B、E2A、E4和VA、单纯疱疹病毒UL29、UL30、UL42、Ul5、UL8、UL52和UL9。在一个实施例中，细胞表达至少一种亲免素类似物(即，亲免素或类似的蛋白质)和至少一种辅助病毒基因产物。
在一些实施例中，使用转录物的替代性mRNA剪接和替代性翻译起始密码子使用以重叠方式从cap开放阅读框(ORF)产生三种AAV衣壳蛋白，即VP1、VP2和VP3。例如，VP1可以从mRNA上的ATG起始密码子(氨基酸M1)翻译而来，而VP2和VP3可以从较短的mRNA中产生(例如，使用不同的起始密码子进行VP2产生并使用通读翻译到下一个可用的密码子进行VP3的产生)。
Cap蛋白可以是VP1和VP3，或者VP1、VP2和VP3。VP1、VP2或VP3基因可以表达以下AAV血清型的衣壳蛋白：AAV1、AAV2、AAV4、AAV5、AAV6、AAV7、AAV8、AAV9、AAV10、AAV11、AAV12、AAV13、AAV-rh.10(AAVrh10)、AAV-DJ(AAVDJ)、AAV-DJ8(AAVDJ8)、AAV-1、AAV-2、AAV-2G9、AAV-3、AAV3a、AAV3b、AAV3-3、AAV4、AAV4-4、AAV-5、AAV-6、AAV6.1、AAV6.2、AAV6.1.2、AAV-7、AAV7.2、AAV8、AAV9、AAV9.11、AAV9.13、AAV9.16、AAV9.24、AAV9.45、AAV9.47、AAV9.61、AAV9.68、AAV9.84、AAV9.9、AAV-10、AAV-11、AAV-12、AAV16.3、AAV24.1、AAV27.3、AAV42.12、AAV42-1b、AAV42-2、AAV42-3a、AAV42-3b、AAV42-4、AAV42-5a、AAV42-5b、AAV42-6b、AAV42-8、AAV42-10、AAV42-11、AAV42-12、AAV42-13、AAV42-15、AAV42-aa、AAV43-1、AAV43-12、AAV43-20、AAV43-21、AAV43-23、AAV43-25、AAV43-5、AAV44.1、AAV44.2、AAV44.5、AAV223.1、AAV223.2、AAV223.4、AAV223.5、AAV223.6、AAV223.7、AAV1-7/rh.48、AAV1-8/rh.49、AAV2-15/rh.62、AAV2-3/rh.61、AAV2-4/rh.50、AAV2-5/rh.51、AAV3.1/hu.6、AAV3.1/hu.9、AAV3-9/rh.52、AAV3-11/rh.53、AAV4-8/r11.64、AAV4-9/rh.54、AAV4-19/rh.55、AAV5-3/rh.57、AAV5-22/rh.58、AAV7.3/hu.7、AAV16.8/hu.10、AAV16.12/hu.11、AAV29.3/bb.1、AAV29.5/bb.2、AAV106.1/hu.37、AAV114.3/hu.40、AAV127.2/hu.41、AAV127.5/hu.42、AAV128.3/hu.44、AAV130.4/hu.48、AAV145.1/hu.53、AAV145.5/hu.54、AAV145.6/hu.55、AAV161.10/hu.60、AAV161.6/hu.61、AAV33.12/hu.17、AAV33.4/hu.15、AAV33.8/hu.16、AAV52/hu.19、AAV52.1/hu.20、AAV58.2/hu.25、AAVA3.3、AAVA3.4、AAVA3.5、AAVA3.7、AAVC1、AAVC2、AAVC5、AAVF3、AAVF5、AAVH2、AAVrh.72、AAVhu.8、AAVrh.68、AAVrh.70、AAVpi.1、AAVpi.3、AAVpi.2、AAVrh.60、AAVrh.44、AAVrh.65、AAVrh.55、AAVrh.47、AAVrh.69、AAVrh.45、AAVrh.59、AAVhu.12、AAVH6、AAVLK03、AAVH-1/hu.1、AAVH-5/hu.3、AAVLG-10/rh.40、AAVLG-4/rh.38、AAVLG-9/hu.39、AAVN721-8/rh.43、AAVCh.5、AAVCh.5R1、AAVcy.2、AAVcy.3、AAVcy.4、AAVcy.5、AAVCy.5R1、AAVCy.5R2、AAVCy.5R3、AAVCy.5R4、AAVcy.6、AAVhu.1、AAVhu.2、AAVhu.3、AAVhu.4、AAVhu.5、AAVhu.6、AAVhu.7、AAVhu.9、AAVhu.10、AAVhu.11、AAVhu.13、AAVhu.15、AAVhu.16、AAVhu.17、AAVhu.18、AAVhu.20、AAVhu.21、AAVhu.22、AAVhu.23.2、AAVhu.24、AAVhu.25、AAVhu.27、AAVhu.28、AAVhu.29、AAVhu.29R、AAVhu.31、AAVhu.32、AAVhu.34、AAVhu.35、AAVhu.37、AAVhu.39、AAVhu.40、AAVhu.41、AAVhu.42、AAVhu.43、AAVhu.44、AAVhu.44R1、AAVhu.44R2、AAVhu.44R3、AAVhu.45、AAVhu.46、AAVhu.47、AAVhu.48、AAVhu.48R1、AAVhu.48R2、AAVhu.48R3、AAVhu.49、AAVhu.51、AAVhu.52、AAVhu.54、AAVhu.55、AAVhu.56、AAVhu.57、AAVhu.58、AAVhu.60、AAVhu.61、AAVhu.63、AAVhu.64、AAVhu.66、AAVhu.67、AAVhu.14/9、AAVhu.t 19、AAVrh.2、AAVrh.2R、AAVrh.8、AAVrh.8R、AAVrh.12、AAVrh.13、AAVrh.13R、AAVrh.14、AAVrh.17、AAVrh.18、AAVrh.19、AAVrh.20、AAVrh.21、AAVrh.22、AAVrh.23、AAVrh.24、AAVrh.25、AAVrh.31、AAVrh.32、AAVrh.33、AAVrh.34、AAVrh.35、AAVrh.36、AAVrh.37、AAVrh.37R2、AAVrh.38、AAVrh.39、AAVrh.40、AAVrh.46、AAVrh.48、AAVrh.48.1、AAVrh.48.1.2、AAVrh.48.2、AAVrh.49、AAVrh.51、AAVrh.52、AAVrh.53、AAVrh.54、AAVrh.56、AAVrh.57、AAVrh.58、AAVrh.61、AAVrh.64、AAVrh.64R1、AAVrh.64R2、AAVrh.67、AAVrh.73、AAVrh.74、AAVrh8R、AAVrh8R A586R突变体、AAVrh8R R533A突变体、AAAV、BAAV、山羊AAV、牛AAV、AAVhE1.1、AAVhEr1.5、AAVhER1.14、AAVhEr1.8、AAVhEr1.16、AAVhEr1.18、AAVhEr1.35、AAVhEr1.7、AAVhEr1.36、AAVhEr2.29、AAVhEr2.4、AAVhEr2.16、AAVhEr2.30、AAVhEr2.31、AAVhEr2.36、AAVhER1.23、AAVhEr3.1、AAV2.5T、AAV-PAEC、AAV-LK01、AAV-LK02、AAV-LK03、AAV-LK04、AAV-LK05、AAV-LK06、AAV-LK07、AAV-LK08、AAV-LK09、AAV-LK10、AAV-LK11、AAV-LK12、AAV-LK13、AAV-LK14、AAV-LK15、AAV-LK16、AAV-LK17、AAV-LK18、AAV-LK19、AAV-PAEC2、AAV-PAEC4、AAV-PAEC6、AAV-PAEC7、AAV-PAEC8、AAV-PAEC11、AAV-PAEC12、AAV-2-pre-miRNA-101、AAV-8h、AAV-8b、AAV-h、AAV-b、AAV SM 10-2、AAV改组100-1、AAV改组100-3、AAV改组100-7、AAV改组10-2、AAV改组10-6、AAV改组10-8、AAV改组100-2、AAV SM10-1、AAV SM 10-8、AAV SM 100-3、AAV SM 100-10、BNP61 AAV、BNP62 AAV、BNP63 AAV、AAVrh.50、AAVrh.43、AAVrh.62、AAVrh.48、AAVhu.19、AAVhu.11、AAVhu.53、AAV4-8/rh.64、AAVLG-9/hu.39、AAV54.5/hu.23、AAV54.2/hu.22、AAV54.7/hu.24、AAV54.1/hu.21、AAV54.4R/hu.27、AAV46.2/hu.28、AAV46.6/hu.29、AAV128.1/hu.43、理想型AAV(ttAAV)、UPENN AAV10或日本AAV10血清型、AAV_po.6、AAV_po.、AAV_po.5、AAV_LK03、AAV_ra.1、AAV_蝙蝠_YNM、AAV_蝙蝠_巴西、AAV_mo.1、AAV_鸟_DA-1、或AAV_小鼠_NY1、Bba21、Bba26、Bba27、Bba29、Bba30、Bba31、Bba32、Bba33、Bba34、Bba35、Bba36、Bba37、Bba38、Bba41、Bba42、Bba43、Bba44、Bce14、Bce15、Bce16、Bce17、Bce18、Bce20、Bce35、Bce36、Bce39、Bce40、Bce41、Bce42、Bce43、Bce44、Bce45、Bce46、Bey20、Bey22、Bey23、Bma42、Bma43、Bpo1、Bpo2、Bpo3、Bpo4、Bpo6、Bpo8、Bpo13、Bpo18、Bpo20、Bpo23、Bpo24、Bpo27、Bpo28、Bpo29、Bpo33、Bpo35、Bpo36、Bpo37、Brh26、Brh27、Brh28、Brh29、Brh30、Brh31、Brh32、Brh33、Bfm17、Bfm18、Bfm20、Bfm21、Bfm24、Bfm25、Bfm27、Bfm32、Bfm33、Bfm34、Bfm35、AAV-rh10、AAV-rh39、AAV-rh43或AAVanc80L65。在另一个实施例中，VP1、VP2或VP3基因表达混合血清型的衣壳，其中VP1、VP2和VP3基因并不全部来自相同血清型。示例性衣壳在整体并入本文的国际申请号WO 2018/022608中提供。
例如，野生型AAV血清型5(AAV-5)的VP1蛋白的氨基酸序列在SEQ ID NO:1中阐明(在没有起始子甲硫氨酸的情况下阐明)：
SFVDHPPDWGLREFLGLEALEEVGPPKGEPKPNQQHQDQARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLEAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEGAKTAPTGKRIDDHFPKRKKARTEEDSKPSTSSDAEAGPSGSQQLQIPAQPASSLGADTMSAGGGGPLGDNNQGADGVGNASGDWHCDSTWMGDRVVTKSTRTWVLPSYNNHQYREIKSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLINNYWGFRPRSLRVKIFNIQVKEVTVQDSTTTIANNLTSTVQVFTDDDYQLPYVVGNGTEGCLPAFPPQVFTLPQYGYATLNRDNTENPTERSSFFCLEYFPSKMLRTGNNFEFTYNFEEVPFHSSFAPSQNLFKLANPLVDQYLYRFVSTNNTGGVQFNKNLAGRYANTYKNWFPGPMGRTQGWNLGSGVNRASVSAFATTNRMELEGASYQVPPQPNGMTNNLQGSNTYALENTMIFNSQPANPGTTATYLEGNMLITSESETQPVNRVAYNVGGQMATNNQSSTTAPATGTYNLQEIVPGSVWMERDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPGNITSFSDVPVSSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNDPQFVDFAPDSTGEYRTTRPIGTRYLTRPL(SEQ ID NO:1)。
在一些实施例中，(AAV病毒颗粒的衣壳的)VP1、VP2和/或VP3蛋白具有如WO 2018/022608或美国专利号9,737,618所公开的氨基酸序列。
在其他实施例中，(AAV病毒颗粒的衣壳的)VP1、VP2和/或VP3蛋白具有在例如狒狒、猪、狨猴、黑猩猩或猕猴中发现的AAV衣壳蛋白的氨基酸序列，其中AAV病毒颗粒用VP1、VP2和/或VP3蛋白假型化。
在一个实施例中，VP1序列(来自狒狒；标示为Bba21)包含SEQ ID NO:2的氨基酸序列(氨基酸1-742)；或者包含在SEQ ID NO:2的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:2的氨基酸138-742，并且VP3衣壳蛋白跨越SEQ ID NO:2的氨基酸206-742。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNEADAAALEHDKAYDQQLKSGDNPYLKYNHADAEFQQRLATDTSFGGNLGKAVFQAKKRILEPLGLVEEGVKTAPGKKRPLEKTPNRPTNPDSGKAPAKKKQKDGETADSARRTLDFEDSGAGDGPPEGSSSGEMSHDAEMRAAPGGNAVEAGQGADGVGNASGDWHCDSTWSEGRVTTTSTRTWVLPTYNNHLYLRIGTTANSNTYNGFSTPWGYFDFNRFHCHFSPRDWQRLINNNWGLRPKSMRVKIFNIQVKEVTTSNGETTVANNLTSTVQIFADSTYELPYVMDAGQEGSLPPFPNDVFMVPQYGYCGVVTGENQNQTDRNAFYCLEYFPSQMLRTGNNFEVSYQFEKVPFHSMYAHSQSLDRMMNPLLDQYLWHPQSTTTGNSLNQGTATTTYGKITTGDFAYYRKNWLPGACIKQQKFSKNASQNYKIPASGGDALLKYDTHTTSNGRWSNMAPGPPMATAGAGDSDFSNSQLIFAGPNQSGNTTTSSNNLLFTSEEEIATTNPRDTDMFGQIADNNQNATTAPHIANLDAMGIVPGMVWQNRDIYYLGPIWAKVPHTDGHFHPSPLMGGFGLKHPPPQIFIKNTPVPANPNTTFSAARINSFLTQYSTGQVAVQIDWEIQKEHSKRWDPEVQFTSNYGTQNSMLWAPDNAGNYHEPRAIGSRFLTHHL(SEQ ID NO:2)。
在另一个实施例中，VP1序列(来自狒狒；标示为Bba26)包含SEQ ID NO:3的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:3的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:3的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:3的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQGNSRGLVLPGYKYLGLFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDDPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFGPQPAAPIGQPPAAPPPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNVQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHGVLVFGQTNATNKTTLDNVLVTSEEEIKATNPVATEEYGTVSSNLQASNTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:3)。
在一些实施例中，VP1序列(来自狒狒；标示为Bba27)包含SEQ ID NO:4的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:4的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:4的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:4的氨基酸206-739。
VAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAELQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPPPVGSNTMASGGGGPMADDNQGADGVGNASGNWHRDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHGVLVFGQTNATNKTTLDNVLITSEEEIKATNPVATEEYGTVSSNLQASNTNPTTETVNSQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENGKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:4)。
在其他实施例中，VP1序列(来自狒狒；标示为Bba29)包含SEQ ID NO:5的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:5的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:5的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:5的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKRRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQCLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHGVLVFGQTNATNKTTLDNVLITSEEEIKATNPVATEEYGTVSSNLQASNTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKEDSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:5)。
在其他实施例中，VP1序列(来自狒狒；标示为Bba30)包含SEQ ID NO:6的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:6的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:6的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:6的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGGQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYLLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHGVLVFGQTNATNKTTLDNVLITSEEEIKATNPVATEEYGTVSSNLQASNTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKEDSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:6)。
在一个实施例中，VP1序列(来自狒狒；标示为Bba31)包含SEQ ID NO:7的氨基酸序列(氨基酸1-742)；或者包含在SEQ ID NO:7的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:7的氨基酸138-742，并且VP3衣壳蛋白跨越SEQ ID NO:7的氨基酸206-742。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNEADAAALEHDKAYDQQLKSGDNPYLKYNHADAEFQQRLATDTSFGGNLGKAVFQAKKRILEPLGLVEEGVKTAPGKKHPLEKTPNRPTNPDSGKAPAKKKQKDGETADSARRTLDFEDSGAGDGPPEGSSSGEMSHDAEMRAAPGGNAVEAGQGADGVGNASGDWHCGSTWSEGRVTTTSTRTWVLPTYNNHLYLRIGTTANSNTYNGFSTPWGYFDFNRFHCHFSPRDWQRLINNNWGLRPKSMRVKIFNIQVKEVTTSNGETTVANNLTSTVQIFADSTYELPYVMDAGQEGSLPPFPNDVFMVPQYGYCGVVTGENQNQTDRNAFYCLECFPSQMLRTGNNFEISYQFEKVPFHSMYAHSQSLDRMMNPLLDQYLWHLQSTTTGNSLNQGTATTTYGKITTGDFAYYRKNWLPGACIKQQKFSKNASQNYKIPASGGDALLKYDTHTTLNGRWSNMAPGPPMATAGAGDSDFSNSQLIFAGPNQSGNTTTSSNNLLFTSEEEIAATNPRDTDMFGQIADNNQNATTAPHIANLDAMGIVPGMVWQNRDIYYQGPIWAKVPHTDGHFHPSPLMGGFGLKHPPPQIFIKNTPVPANPNTTFSAARINSFLTQYSTGQVAVQIDWEIQKEHSKRWNPEVQFTSNYGTQNSMLWAPDNAGNYHEPRAIGSRFLTHHL(SEQ ID NO:7)。
在另一个实施例中，VP1序列(来自狒狒；标示为Bba32)包含SEQ ID NO:8的氨基酸序列(氨基酸1-742)；或者包含在SEQ ID NO:8的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:8的氨基酸138-742，并且VP3衣壳蛋白跨越SEQ ID NO:8的氨基酸206-742。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNEADAAALEHDKAYDQQLKSGDNPYLKYNHADAEFQQRLATDTSFGGNLGKAVFQAKKRILEPLGLVEEGVKTAPGKKRPLEKTPNRPTNPDSGKAPAKKKQKDGETADSARRTLDFEDSGAGDGPPEGSSSGEMSHDAEMRAAPGGNAVEAGQGADGVGNASGGWHCDSTWSEGRVTTTSTRTWVLPTYNNHLYLRIGTTANSNTYNGFSTPWGYFDFNRFHCHFSPRDWQRLINNNWGLRPKSMRVKIFNIQVKEVTTSNGETTVANNLTSTVQIFADSTYELPYVMDAGQEGSLPPFPNDVFMVPQYGYCGVVTGENQNQTDRNAFYCLEYFPSQMLRTGNNFEISYQFEKVPFHSMYAHSQSLDRMMNPLLDQYLWHLQSTTTGNSLNQGTATTTYGKITTGDFAYYRKNWLPGACIKQQKFSKNASQNYKIPASGGDALLKYDTHTTLNGRWSSMAPGPPMATAGAGDSDFSNSQLIFAGPNQSGNTTTSSNNLLFTSEEEIATTNPRDTDMFGQIADNNQNATTAPHIANLDAMGIVPGMVWQNRDIYYQGPIWAKVPHTDGHFHPSPLMGGFGLKHPPPQIFIKNTPVPANPNTTFSAARINSFLTQYSTGQVAVQIDWEIQKEHSKRWNPEVQFTSNYGTQNSMLWAPGNAGNHHEPRAIGSRFLTHHL(SEQ ID NO:8)。
在一些实施例中，VP1序列(来自狒狒；标示为Bba33)包含SEQ ID NO:9的氨基酸序列(氨基酸1-742)；或者包含在SEQ ID NO:9的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:9的氨基酸138-742，并且VP3衣壳蛋白跨越SEQ ID NO:9的氨基酸206-742。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNEADAAALEHDKAYDQQLKSGDNPYLKYNHADAEFQQRLATDTSFGGNLGKAVFQAKKRILEPLGLVEEGVKTAPGKKRPLEKTPNRPTNPDSGRAPAKKKQKDGETADSARRTLDFEDSGAGDGPPEGSSSGEMSHDAEMRAAPGGNAVEAGQGADGVGNASGDWHCDSTWSEGRVTTTSTRTWVLPTYNNHLYLRIGTTANSNTYNGFSTPWGYFDFNRFHCHFSPRDWQRLINNNWGLRPKSMRVKIFNIQVKEVTTSNGETTVANNLTSTVQIFADSTYELPYVMDAGQEGSLPPFPNDVFMVPQYGYCGVVTGENQNQTDRNAFYCLEYFPSQMLRTGNNFEISYQFEKVPFHSMYAHSQSLDRMMNPLLDQYLWHLQSTTTGNSLNQGTATTTYGKITTGDFAYYRKNWLPGACIKQQKFSKNASQNYKIPASGGDALLKYDTHTTLNGRWSNMAPGPPMATAGAGDSDFSNSQLIFAGPNQSGNTTTSSNNLLLTSEEEIATTNPRDTDMFGQIADNNQNATTAPHIANLDAMGIVPGMVWQNRDIYYQGPIWAKVPHTDGHFHPSPLMGGFGLKHPPPQIFIKNTPVPANPNTTFSAARINSFLTQYSTGQVAVQIDWEIQKEHSKRWNPEVQFTSNYGTQNSMLWAPGNAGNYHEPRAIGSRFLTHHL(SEQ ID NO:9)。
在其他实施例中，VP1序列(来自狒狒；标示为Bba34)包含SEQ ID NO:10的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:10的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:10的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:10的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGILGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLVNPGIAMATHKDDEERFFPQHGVLVFGKTNATNKTTLENVLVTDEEEVKATNPVATEEYGTVSSNLQSNTTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSADAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:10)。
在一个实施例中，VP1序列(来自狒狒；标示为Bba35)包含SEQ ID NO:11的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:11的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:11的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:11的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEVAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHRVLVFGQTNATNKTTLDNVLITSEEEIKATNPVATEEYGTVSSNLQASNTDPTTETVNNQGILPGMVWQDRDVYLQGPIWVKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYTTQYSTGQVSVEIEWGLQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:11)。
在另一个实施例中，VP1序列(来自狒狒；标示为Bba36)包含SEQ ID NO:12的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:12的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:12的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:12的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEAYAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRIPEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRPSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHGVLVFGQTNATNKTTLDNVLITSEEEIKATNPVATEEYGTVSSNLQASNTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:12)。
在一些实施例中，VP1序列(来自狒狒；标示为Bba37)包含SEQ ID NO:13的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:13的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:13的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:13的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAETAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQTEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIINPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHGVLVFGQTNATNKTTLDNVLITSEEEIKATNPVATEEYGTVSSNLQASNTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:13)。
在其他实施例中，VP1序列(来自狒狒；标示为Bba38)包含SEQ ID NO:14的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:14的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:14的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:14的氨基酸206-739。
MAADGYLPDWLEDNLSEGIRGWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPCLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHGVLVFGQTNATNKTTLDNVLITSEEEIKATNPVATEEYGTVSSNLQASNTNPTTETVNNQGILPGMVWQDRDVYLQGPTWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:14)。
在一个实施例中，VP1序列(来自狒狒；标示为Bba41)包含SEQ ID NO:15的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:15的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:15的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:15的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNSFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLANPGIAMATHKDDEERFFPQHGVLVFGQTNATNKTTLDNVLITSEEEIKATNPVATEEYGTVSSNLQASNTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:15)。
在另一个实施例中，VP1序列(来自狒狒；标示为Bba42)包含SEQ ID NO:16的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:16的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:16的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:16的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNGADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLVNPGIAMATHKDDEERFFPQHGVLVFGKTNATNKTTLENVLVTDEEEVKATNPVATEEYGTVSSNLQSNTTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRLIGTRYLTRNL(SEQ ID NO:16)。
在一些实施例中，VP1序列(来自狒狒；标示为Bba43)包含SEQ ID NO:17的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:17的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:17的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:17的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQNSNTNFAWTAASKYNLNGRKSLVNPGIAMATHKDDEERFFPQHGVLVFGKTNATNKTTLENVLVTDEEEVKATNPVATEEYGTVSSNLQSNTTNPTTETVNNQGILPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:17)。
在其他实施例中，VP1序列(来自狒狒；标示为Bba44)包含SEQ ID NO:18的氨基酸序列(氨基酸1-739)；或者包含在SEQ ID NO:18的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:18的氨基酸138-739，并且VP3衣壳蛋白跨越SEQ ID NO:18的氨基酸206-739。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDKQLEQGDNPYLKYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPLEKTPNQPTDTQAAGQTPAKKRPQGEQSGDSARRQLDFDPQPAAPIGQPPAAPSPVGSNTMASGGGGPMADDNQGADGVGNASGNWHCDSTWLGDRVITTSTRTWVLPTYNNHIYKQISSESGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKIFNIQVKEVTQIEGGSTIANNLTSTIQVFADSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFQFSYTFESVPFHSSYAHSQSLDRIMNPLVDQYLYYLARTQTGTGSTTSNTRQLQFYQAGPSNMADQSRNWLPGPMYRQQRVSKTLDQDSNTNFAWTAASKYNLNGRKSLVNPGIAMATHKDDEERFFPQHGVLVFGKTNATNKTTLENVLVTDEEEVKATNPVATEEYGTVSSNLQSNTTNPTTETVNNQGILPGMAWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTTSKFASYITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYAKSNNVEFSVDAAGVYSEPRPIGTRYLTRNL(SEQ ID NO:18)。
在一个实施例中，VP1序列(来自食蟹猕猴(crab-eating macaque)；标示为Bce14)包含SEQ ID NO:19的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:19的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:19的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:19的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQALKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLVGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVRVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:19)。
在另一个实施例中，VP1序列(来自食蟹猕猴；标示为Bce15)包含SEQ ID NO:20的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:20的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:20的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:20的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMPRTGNNFTFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVAIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:20)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce16))包含SEQ ID NO:21的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:21的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:21的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:21的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYEFENVPFHSSYAHSQSLDRPMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:21)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce17))包含SEQ ID NO:22的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:22的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:22的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:22的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAIKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSIRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFSRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:22)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce18))包含SEQ ID NO:23的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:23的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:23的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:23的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:23)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce20))包含SEQ ID NO:24的氨基酸序列(氨基酸1-733)；或者包含在SEQ ID NO:24的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:24的氨基酸138-733，并且VP3衣壳蛋白跨越SEQ ID NO:24的氨基酸203-733。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSRWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGAKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRP IGTRYLT(SEQ ID NO:24)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce35))包含SEQ ID NO:25的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:25的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:25的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:25的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVLQAKKRVLEPLGLVEEAAKTAPGKKRPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNASGNWHCDSTWLGNRVITTSTRTWALPTYNNHLYKQISSSSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLARTQSSTGTSTRELQFHQAGPATMAEQSKNWLPGPCFRQQRISKTTDNNNNSNFAWTGATKYHLNGRSSLTNPGVPMATHKDDESVFFPINGVLVFGKTGASNKTTLENVLMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPTTQTVNNQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYDKQTGVGFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:25)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce36))包含SEQ ID NO:26的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:26的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:26的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:26的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNASGNWHCDSTWLGNRVITTSTRTWALPTYNNHLYKQISSSSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLARTQSSTGTSTRELQFHQAGPATMAEQSKNWLPGPCFRQQRISKTTDNNNNSNFAWTGATKYHLNGRNSLTNPGVPMATHKDDESVFFPINGVLVFGKTGASNKTTLENVLMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPTTQTVNNQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYDKQTGVDFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:26)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce39))包含SEQ ID NO:27的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:27的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:27的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:27的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYPGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVFEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPSYRQQRVSTTKNQNNNSNFAWTGAAKFKLNGRNSLMNPGVAMASHKDDEDRFFPSSGVLIFGKQGAGNDGVDYSQVLITDEEEIKATNPVATEEYGEVAINDQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLMHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNSDGVYSEPRPIGTRYLTRNL(SEQ ID NO:27)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce40))包含SEQ ID NO:28的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:28的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:28的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:28的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQGRLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRPNFKLFNIQVKEVTTNEGTKTIANNLTSTVQAFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPSYRQQRVSTTKNQNNNSNFAWTGAAKFKLNGRNSLMNPGVAMASHKDDEDRFSPSSGVLIFGKQGAGNDGVDYSQVLITDEEEIKATNPVATEEYGEVAINDQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNSDGVYSEPRP IGTRYLTRNL(SEQ ID NO:28)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce41))包含SEQ ID NO:29的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:29的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:29的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:29的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQGRLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQILAFSQAGPSSMANQARNWVPGPSYRQQRVSTTKNQNNNSNFAWTGAAKFKLNGRNSLMNPGVAMASHKDDEDRFFPSSGVLIFGKQGAGNDGMDYSQVLITDEEEIKATNPVATEEYGEVAINDQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNSDGVYSEPRPIGTRYLTRNL(SEQ ID NO:29)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce42))包含SEQ ID NO:30的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:30的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:30的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:30的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGGTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGHLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPSYRQQRVSTTKDQNNNSNFAWTGAAKFKLNGRNSLMNPGVAMASHKDDEDRFFPSSGVLIFGKQGAGNDGVDYSQVLITDEEEIKATNPVATEEYGEVAVNDQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTLNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNSDGVYSEPRPIGTRYLTRNL(SEQ ID NO:30)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce43))包含SEQ ID NO:31的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:31的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:31的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:31的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNAPGNWHCDSTWLGNRVITTSTRTWALPTYNNHLYKQISSSSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWPRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYPYYLARTQSSTGTSTRELQFHQAGPATMAEQSKNWLPGPCFRQQRISKTTDNNNNSNFAWTGATKYHLNGRNSLTNPGVPMATHKDDESVFFPINGVLVFGKTGASNKTTLENVLMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPATQTVNNQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYDKQTGVDFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:31)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce44))包含SEQ ID NO:32的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:32的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:32的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:32的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLMLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNASGSWHCDSTWLGNRVITTSTRTWALPTYNNHLYKQISSSSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGRQSVGRSSFYCLEYFPSQMLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYPYYLARTQSSTGTSTRELQFHQAGPATMAEQSKNWLPGPCFRQQRISKTTDNNNNSNFAWTGATKYHLNGRNSLTNPGVPMATHKDDESVFFPINGVLVFGKTGASNKTTLENVPMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPTTQTVNNQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENNKRWNPEIQYTSNYDKQTGVDFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:32)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce45))包含SEQ ID NO:33的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:33的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:33的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:33的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFSGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNASGNWHCDSTWLGNRVITTSTRTWALPTYNDHLYKQISSSSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLARTQSSTGTSTRELQFHQAGPATMAEQSKNWLPGPCFRQQRISKTTDNNNNSNFAWTGATKYHLNGRNSLTNPGVPMATHKDDESVFFPINGVLVFGKTGASNKTTLENVLMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPTTQTVNNQGALPGMVWRNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYDKQTGVDFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:33)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bce46))包含SEQ ID NO:34的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:34的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:34的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:34的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNASGNWHCDSTWLGNRVITTSTRTWALPTYNNHLYKQISSSSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYMARTQSSTGTSTREPQFHQAGPATMAEQSKNWLPGPCFRQQRISKTTDNNNNSNFAWTGATKYHLNGRNSLTNPGVPMATHKDDESVFFPINGVLVFGKTGASNKTTLENVLMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPTTQTVNNQGALPGMVWQNRDVYLQGPIWAKIPHTDGDFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYDKQTGVDFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:34)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bey20))包含SEQ ID NO:35的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:35的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:35的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:35的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNASGNWHCDSTWLGNRVITTSTRTWALPTYNNHLYKQISSGSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQVLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLARTQSSTGTSTRELQFHQAGPATMAEQSKNWLPGPCFRQQRISKTSDNNNNSNFAWTGATKYHLNGRNSLTNPGVPMATHKDDESVFFPINGVLVFGKTGASNKTTLENVLMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPTTQTVNNQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYDKQTGVDFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:35)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bey22))包含SEQ ID NO:36的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:36的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:36的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:36的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQKQDDGRGLVLPGYRYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKERPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNASGNWHCDSTWLGNRVITTSTRTWALPTYDNHLYKQISSSSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYCLEYFPSQMLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLARTQSSTGTSTRELQFHQAGPATMAEQSKNWLPGPCFRQQRISKTTDNNNNSNFAWTGATKYHLNGRNSLTNPGVPMATHKDDESVFFPINGVLVLGKTGASNKTTLENVLMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPTTQTVNNQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYDKQTGVDFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:36)。
在另一个实施例中，VP1序列(来自食蟹猕猴(标示为Bey23))包含SEQ ID NO:37的氨基酸序列(氨基酸1-730)；或者包含在SEQ ID NO:37的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:37的氨基酸138-730，并且VP3衣壳蛋白跨越SEQ ID NO:37的氨基酸199-730。
MAADGYLPDWLEDNLSEGIREWWALKPGAPRPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEAAKTAPGKKRPVDSPDSTSGIGKKGQQPARKRLNFGQTGDAESVPDPQPIGEPPAAPSGLGSGTMAAGGGAPMADNNEGADGVGNASGNWHCDSTWLGNRVITTSTRTWALPTYNNHLYKQISSSSSGATNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLRFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQSVGRSSFYRLEYFPSQMLRTGNNFEFSYEFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLARTQSSTGTSTRELQFHQAGPATMAEQSKNWLPGPCFRQQRISKTTDNNNNSNFAWTGATKYHLNGRNSLTNPGVPMATHRDDESVFFPINGVLVFGKTGASNKTTLENVLMTDEEEIKATNPVATEEYGVVSSNIQSQNSNPTTQTVNNQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPANPPETFTPAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYDKQTGVDFAVDTQGVYSEPRPIGTRYLTRNL(SEQ ID NO:37)。
在另一个实施例中，VP1序列(来自狨猴(标示为Bma42))包含SEQ ID NO:38的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:38的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:38的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:38的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDTESVPDPQPLREPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKPFNIQVKEVTTNEGTKTIANNPTSTVQVFTDSEYQLPYVLGSARQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGDNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDEDRFFPSSGVLIFGKQGAGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTLAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:38)。
在另一个实施例中，VP1序列(来自狨猴(标示为Bma43))包含SEQ ID NO:39的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:39的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:39的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:39的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGANTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLREPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINSNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDEDRFFPSSGVLIFGKQGAGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:39)。
在另一个实施例中，VP1序列(来自猪(标示为Bpol))包含SEQ ID NO:40的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:40的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:40的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:40的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKEDSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:40)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo2))包含SEQ ID NO:41的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:41的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:41的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:41的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRALEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVGTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:41)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo3))包含SEQ ID NO:42的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:42的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:42的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:42的氨基酸184-716。
MSFVDHPPDWFEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPANRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFGEVPLHCSFAPSQNLFKLANPLVDQHLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRPGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLTKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:42)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo4))包含SEQ ID NO:43的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:43的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:43的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:43的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:43)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo6))包含SEQ ID NO:44的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:44的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:44的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:44的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHGISYNEQLQAGDDPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTGGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSLEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:44)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo8))包含SEQ ID NO:45的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:45的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:45的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:45的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLKPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLGRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQANPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSLITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:45)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo13))包含SEQ ID NO:46的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:46的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:46的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:46的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLASTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:46)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo18))包含SEQ ID NO:47的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:47的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:47的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:47的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDDPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMRRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:47)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo20))包含SEQ ID NO:48的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:48的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:48的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:48的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWGPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEGVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:48)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo23))包含SEQ ID NO:49的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:49的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:49的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:49的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKRDNARGLVLPGYNYLGPGNGLDRGEPVNRVDEVARERDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPLQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:49)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo24))包含SEQ ID NO:50的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:50的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:50的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:50的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGECRTTRAIGTRYLTRPL(SEQ ID NO:50)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo27))包含SEQ ID NO:51的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:51的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:51的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:51的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVDNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMVFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYNTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:51)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo28))包含SEQ ID NO:52的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:52的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:52的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:52的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKRDNARGLVLPGYNYLGPGNGLGRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGLRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLADQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMISNSQNAEPGTTSLYRENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:52)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo29))包含SEQ ID NO:53的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:53的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:53的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:53的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESGTQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRGVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFVTQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:53)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo33))包含SEQ ID NO:54的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:54的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:54的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:54的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNAHAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:54)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo35))包含SEQ ID NO:55的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:55的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:55的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:55的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKRDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVGFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:55)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo36))包含SEQ ID NO:56的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:56的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:56的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:56的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLEPGPPKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIGDHYPKKKKARVEETEAGTSGGQQPQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYLPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTSGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQVNPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYNTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:56)。
在另一个实施例中，VP1序列(来自猪(标示为Bpo37))包含SEQ ID NO:57的氨基酸序列(氨基酸1-716)；或者包含在SEQ ID NO:57的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:57的氨基酸137-716，并且VP3衣壳蛋白跨越SEQ ID NO:57的氨基酸184-716。
MSFVDHPPDWLEEIGEGLKEFLGLKPGPLKPKPNQQKQDNARGLVLPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLQAGDNPYLKYNHADAEFQEKLADDTSFGGNLGKAVFQAKKRVLEPFGLVEEPAKTAAKGERIDDHYPKKKKARVEETEAGTSGGQQLQIPAQPASSLGADTMSAGGGSPLGDNNQGADGVGNASGDWHCDSTWMGDRVITKSTRTWVLPSYNNHLYKEIHSGSVDGSNANAYFGYSTPWGYFDFNRFHSHWSPRDWQRLVNNYWGFRPRSLKVKIFNIQVKEVTVQDATTTIANNLTSTVQVFTDDDYQLPYVIGNGTEGCLPAFPPQVFTLPQYGYATLNRNNTDDPTERSSFFCLEYFPSKMLRTGNSFEFTYSFEEVPFHCSFAPSQNLFKLANPLVDQYLYRFVSTDTPGNIQFQKNLKARYANTYKNWFPGPMCRTQGWYTGSGTYNRSGVTNFATSNRMDLEGASYQANPQPNGMTNTLQDSNKYALENTMIFNSQNAEPGTTSLYQENNLLITSESETQPVNRVAYDTGGQMATNAQSTNLAPTVGTYNHQEMLPGSVWMDRDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPMMLIKNTPVPSNVTAFSEIPVKSLITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYNNPEFVDFAPDTSGEYRTTRAIGTRYLTRPL(SEQ ID NO:57)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Brh26))包含SEQ ID NO:58的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:58的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:58的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:58的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPANAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAIFQAKKRVLEPLGLVEEGAKTAPGKKGPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDDDRFFPSSGVLIFGKQGTGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:58)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Brh27))包含SEQ ID NO:59的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:59的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:59的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:59的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAIFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNPSGNWHCDSTRLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTSNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDDDRFFPSSGVLIFGKQGTGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKLPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:59)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Brh28))包含SEQ ID NO:60的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:60的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:60的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:60的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAIFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSPSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDDDRFFPSSGVLIFGKQGTGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:60)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Brh29))包含SEQ ID NO:61的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:61的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:61的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:61的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAIFQAKRRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTSNNFQFSYTFEDVPFHSSCAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPGSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDDDRFFPSSGVLIFGKQGTGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPPPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:61)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Brh30))包含SEQ ID NO:62的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:62的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:62的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:62的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVFPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAIFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGRQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTPGGSTNDNTYFGYSAPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDDDRFFPSSGVLIFGKQGTGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYQQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRP IGTRYLTRNL(SEQ ID NO:62)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Brh31))包含SEQ ID NO:63的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:63的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:63的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:63的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAIFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNSGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAYQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQSSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDDDRFFPSSGVLIFGKQGTGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:63)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Brh32))包含SEQ ID NO:64的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:64的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:64的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:64的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGASKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTVPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAYQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDDDRFFPSSGVLIFGKQGTGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:64)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Brh33))包含SEQ ID NO:65的氨基酸序列(氨基酸1-736)；或者包含在SEQ ID NO:65的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:65的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:65的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWDLKPGASKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTVPGKKRPVEQSPQEPDSSSGIGKTGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSGLGPNTMASGGGAPMADNNEGADGVGNSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNEGTKTIANNLTSTVQVFTDSEYQLPYVLGSAYQGCLPPFPADVFMVPQYGYLTLNNGSQALGRSSFYCLEYFPSQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLVRTQTTGTGGTQTLAFSQAGPSSMANQARNWVPGPCYRQQRVSTTTNQNNNSNFAWTGAAKFKLNGRDSLMNPGVAMASHKDDDDRFFPSSGVLIFGKQGTGNDGVDYSQVLITDEEEIKATNPVATEEYGAVAINNQAANTQAQTGLVHNQGVIPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPLTFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRP IGTRYLTRNL(SEQ IDNO:65)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm17))包含SEQ ID NO:66的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:66的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:66的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:66的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTRLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLSYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQEALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVDTEGVYSEHRPIGTRYLTRNL(SEQ ID NO:66)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm18))包含SEQ ID NO:67的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:67的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:67的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:67的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVPGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYPQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:67)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm.20))包含SEQ ID NO:68的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:68的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:68的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:68的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPPGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSFFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIERELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:68)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm21))包含SEQ ID NO:69的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:69的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:69的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:69的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKERPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSAVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVTTEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTESVYSEPRPIGTRYLTRNL(SEQ ID NO:69)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm24))包含SEQ ID NO:70的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:70的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:70的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:70的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGPDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRSHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEIRTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:70)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm25))包含SEQ ID NO:71的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:71的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:71的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:71的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRLFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEVKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:71)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm27))包含SEQ ID NO:72的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:72的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:72的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:72的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKRRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSARQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVDTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:72)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm32))包含SEQ ID NO:73的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:73的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:73的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:73的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFDIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:73)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm33))包含SEQ ID NO:74的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:74的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:74的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:74的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSAVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTESVYSEPRPIGTRYLTRNL(SEQ ID NO:74)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm34))包含SEQ ID NO:75的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:75的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:75的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:75的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFSFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVYPGVAMATNKDDEDRFFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:75)。
在另一个实施例中，VP1序列(来自恒河猴(标示为Bfm35))包含SEQ ID NO:76的氨基酸序列(氨基酸1-737)；或者包含在SEQ ID NO:76的氨基酸序列的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。VP2衣壳蛋白跨越SEQ ID NO:76的氨基酸138-736，并且VP3衣壳蛋白跨越SEQ ID NO:76的氨基酸203-736。
MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGGPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFRERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEQSPQEPDSSSGIGKKGQQPAKKRLNFGQTGDSESVPDPQPLGEPPAAPSSLGLNTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDSTYFGYSTPWGYFDFNRSHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGGQAVGRSSFYCLEYFPSQMLRTGNNFSFSYIFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPMYRQQRVSTTLSQNNNSNFAWTGGTKYHLNGRDSLVNPGVAMATNKDDEDRFFPSSGVLMFGKQGAGKDNVDYSSVMLTSEEEIKTTNPVATEQYGVVADNLQQQNTAPIVGAVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTAFNQAKLNSFITQYSTGQVSVEIEWELLKESSKRWNPEIQYTSNYYKSTNVDFAVNTEGVYSEPRPIGTRYLTRNL(SEQ ID NO:76)。
在一个实施例中，AAV病毒颗粒是血清型1(AAV-1)、血清型2(AAV-2)、血清型3(AAV-3)、血清型3B(AAV-3B)、血清型4(AAV-4)、血清型5(AAV-5)、血清型6(AAV-6)、血清型7(AAV-7)、血清型8(AAV-8)、血清型9(AAV-9)、血清型10(AAV-10)、血清型11(AAV-11)、血清型12(AAV-12)或血清型13(AAV-13)。
在另一个实施例中，AAV病毒颗粒用源自人、狒狒、狨猴、猪、黑猩猩或猕猴的AAV颗粒的衣壳蛋白假型化。
在一些实施例中，AAV病毒颗粒用源自来自狒狒的AAV颗粒的衣壳蛋白假型化。
在其他实施例中，AAV病毒颗粒用Bba.21、Bba.26、Bba.27、Bba.29、Bba.30、Bba.31、Bba.32、Bba.33、Bba.34、Bba.35、Bba.36、Bba.37、Bba.38、Bba.41、Bba.42、Bba.43、Bba.44、Bce.14、Bce.15、Bce.16、Bce.17、Bce.18、Bce.20、Bce.35、Bce.36、Bce.39、Bce.40、Bce.41、Bce.42、Bce.43、Bce.44、Bce.45、Bce.46、Bey.20、Bey.22、Bey.23、Bma.42、Bma.43、Bpo.1、Bpo.2、Bpo.3、Bpo.4、Bpo.6、Bpo.8、Bpo.13、Bpo.18、Bpo.20、Bpo.23、Bpo.24、Bpo.27、Bpo.28、Bpo.29、Bpo.33、Bpo.35、Bpo.36、Bpo.37、Brh.26、Brh.27、Brh.28、Brh.29、Brh.30、Brh.31、Brh.32、Brh.33、Bfm.17、Bfm.18、Bfm.20、Bfm.21、Bfm.24、Bfm.25、Bfm.27、Bfm.32、Bfm.33、Bfm.34或Bfm.35衣壳假型化。
在一个实施例中，AAV病毒颗粒是血清型1(AAV-1)、血清型2(AAV-2)、血清型3(AAV-3)、血清型3B(AAV-3B)、血清型4(AAV-4)、血清型5(AAV-5)、血清型6(AAV-6)、血清型7(AAV-7)、血清型8(AAV-8)、血清型9(AAV-9)、血清型10(AAV-10)、血清型11(AAV-11)、血清型12(AAV-12)或血清型13(AAV-13)，其中AAV颗粒用源自人、狨猴、狒狒、黑猩猩或猕猴的衣壳蛋白假型化。
在另一个实施例中，AAV病毒颗粒是血清型1(AAV-1)、血清型2(AAV-2)、血清型3(AAV-3)、血清型3B(AAV-3B)、血清型4(AAV-4)、血清型5(AAV-5)、血清型6(AAV-6)、血清型7(AAV-7)、血清型8(AAV-8)、血清型9(AAV-9)、血清型10(AAV-10)、血清型11(AAV-11)、血清型12(AAV-12)或血清型13(AAV-13)，其中AAV颗粒用Bba.21、Bba.26、Bba.27、Bba.29、Bba.30、Bba.31、Bba.32、Bba.33、Bba.34、Bba.35、Bba.36、Bba.37、Bba.38、Bba.41、Bba.42、Bba.43、Bba.44、Bce.14、Bce.15、Bce.16、Bce.17、Bce.18、Bce.20、Bce.35、Bce.36、Bce.39、Bce.40、Bce.41、Bce.42、Bce.43、Bce.44、Bce.45、Bce.46、Bey.20、Bey.22、Bey.23、Bma.42、Bma.43、Bpo.1、Bpo.2、Bpo.3、Bpo.4、Bpo.6、Bpo.8、Bpo.13、Bpo.18、Bpo.20、Bpo.23、Bpo.24、Bpo.27、Bpo.28、Bpo.29、Bpo.33、Bpo.35、Bpo.36、Bpo.37、Brh.26、Brh.27、Brh.28、Brh.29、Brh.30、Brh.31、Brh.32、Brh.33、Bfm.17、Bfm.18、Bfm.20、Bfm.21、Bfm.24、Bfm.25、Bfm.27、Bfm.32、Bfm.33、Bfm.34或Bfm.35衣壳蛋白或其变体假型化。
在其他实施例中，AAV病毒颗粒用具有VP1蛋白的衣壳假型化，该蛋白包含SEQ IDNO:2-76中的任何一个的氨基酸序列；或者包含在SEQ ID NO:2-76中的任何一个的全长上至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％或至少95％相同的氨基酸序列。
在任何方面或实施例中，VP1、VP2或VP3的任何序列都在掺入到衣壳中之前进行翻译后修饰。例如，将任何序列VP1、VP2或VP3乙酰化，其中序列的位置1中的甲硫氨酸被移除并用乙酰基替代。
过渡金属或盐
盐可以是有机盐或无机盐。在一些实施例中，盐是金属盐。
在其他实施例中，盐包含过渡金属。盐包含过渡金属的任何离子(例如，铜(I)、铜(II)、铁(II)、铁(III))。
在各种实施例中，过渡金属是铜、钪、钛、钒、铬、锰、铁、钴、镍、铜、锌、钇、锆、铌、钼、锝、钌、铑、钯、银、镉、铪、钽、钨、铼、锇、铱、铂、金、汞、 或/>
在另一个实施例中，过渡金属是铜。
在各种实施例中，过渡金属或盐是硫酸铜、硝酸铜、硒化铜、氢氧化铜、氧化铜、磷酸铜、硅酸铜、硼酸铜、碳酸铜、氯化铝、氯化镁、硒化锂、碳酸钠、氯化锂、磷酸氢钠、偏硅酸钠、氢氧化锶、磷酸三钠、氟化钾、硫酸镁、氯化钙、硫酸钠、硫酸铝、四硼酸钠、硫酸镁、溴化镁、硫酸铝铷、氢氧化钡、硫酸铝钾、硝酸镁、磷酸氢钠、硫酸镍、硫酸锌、硫酸铍、硝酸锂、氯化锶、硝酸锌、焦磷酸钠、溴化钙、硝酸铜、硝酸铝、四硼酸钠、氟化银、碘化钙、溴化锂、碘化锂、溴化锶、硝酸钙、碘化锶、溴化钠和硝酸锶、乳酸钠铝、乙酸钠、脱氢乙酸钠、丁氧基乙氧基乙酸钠、辛酸钠、柠檬酸钠、乳酸钠、二羟基甘氨酸钠、葡萄糖酸钠、谷氨酸钠、羟基甲烷磺酸钠或草酸钠。盐包括盐的水合物和无水盐。
在其他实施例中，盐是铜盐。
在一些实施例中，可以以铜盐、铜螯合物或其组合的形式将铜添加到培养基中。
在一个实施例中，铜盐是硫酸铜、硝酸铜、硒化铜、氢氧化铜、氧化铜、磷酸铜、硅酸铜、硼酸铜或碳酸铜。
在另一个实施例中，铜盐是硫酸铜。
有效量
在一些实施例中，与掺入到由在除了培养基中没有有效量的铜外类似或基本上类似的培养条件下培养的宿主细胞产生的AAV病毒颗粒的衣壳中的VP1的量相比，培养基中盐的有效量足以增加VP1到由宿主细胞产生的AAV病毒颗粒的衣壳中的掺入。
掺入衣壳中的VP1的量可以通过本领域普通技术人员已知的一种或多种方法来检测和定量。这些可以包括分析生物化学方法诸如电泳、毛细管电泳、高效液相色谱法(HPLC)、薄层色谱法(TLC)、超扩散色谱法等、或各种免疫学方法诸如流体或凝胶沉淀反应、免疫扩散、免疫电泳、放射免疫测定(RIA)、酶联免疫吸附测定(ΕLISA)、免疫荧光测定、蛋白质印迹等。
在一个实施例中，培养基中盐的总浓度为约1nM至约1mM，说明性地约5nM至约900μM、约10nM至约800μM、约20nM至约700μM、约30nM至约600μM、约40nM至约500μM、约50nM至约400μM、约60nM至约300μM、约70nM至约200μM、约80nM至约100μM、以及约90nM至约95nM。
在各种实施例中，培养基中过渡金属或盐的总浓度为1nM、2nM、3nM、4nM、5nM、6nM、7nM、8nM、9nM、10nM、11nM、12nM、13nM、14nM、15nM、16nM、17nM、18nM、19nM、20nM、21nM、22nM、23nM、24nM、25nM、26nM、27nM、28nM、29nM、30nM、31nM、32nM、33nM、34nM、35nM、36nM、37nM、38nM、39nM、40nM、41nM、42nM、43nM、44nM、45nM、46nM、47nM、48nM、49nM、50nM、51nM、52nM、53nM、54nM、55nM、56nM、57nM、58nM、59nM、60nM、61nM、62nM、63nM、64nM、65nM、66nM、67nM、68nM、69nM、70nM、71nM、72nM、73nM、74nM、75nM、76nM、77nM、78nM、79nM、80nM、81nM、82nM、83nM、84nM、85nM、86nM、87nM、88nM、89nM、90nM、91nM、92nM、93nM、94nM、95nM、96nM、97nM、98nM、99nM、100nM、101nM、102nM、103nM、104 nM、105 nM、106 nM、107 nM、108 nM、109 nM、110 nM、111 nM、112 nM、113nM、114 nM、115 nM、116 nM、117 nM、118 nM、119 nM、120 nM、121 nM、122 nM、123 nM、124 nM、125 nM、126 nM、127 nM、128 nM、129 nM、130 nM、131 nM、132nM、133 nM、134 nM、135 nM、136 nM、137 nM、138 nM、139 nM、140 nM、141 nM、142 nM、143 nM、144 nM、145 nM、146 nM、147 nM、148 nM、149 nM、150 nM、151nM、152 nM、153 nM、154 nM、155 nM、156 nM、157 nM、158 nM、159 nM、160 nM、161 nM、162 nM、163 nM、164 nM、165 nM、166 nM、167 nM、168 nM、169 nM、170nM、171 nM、172 nM、173 nM、174 nM、175 nM、176 nM、177 nM、178 nM、179 nM、180 nM、181 nM、182 nM、183 nM、184 nM、185 nM、186 nM、187 nM、188 nM、189nM、190 nM、191 nM、192 nM、193 nM、194 nM、195 nM、196 nM、197 nM、198 nM、199 nM、200 nM、201 nM、202 nM、203 nM、204 nM、205 nM、206 nM、207 nM、208nM、209 nM、210 nM、211 nM、212 nM、213 nM、214 nM、215 nM、216 nM、217 nM、218 nM、219 nM、220 nM、221 nM、222 nM、223 nM、224 nM、225 nM、226 nM、227nM、228 nM、229 nM、230 nM、231 nM、232 nM、233 nM、234 nM、235 nM、236 nM、237 nM、238 nM、239 nM、240 nM、241 nM、242 nM、243 nM、244 nM、245 nM、246nM、247 nM、248 nM、249 nM、250 nM、251 nM、252 nM、253 nM、254 nM、255 nM、256 nM、257 nM、258 nM、259 nM、260 nM、261 nM、262 nM、263 nM、264 nM、265nM、266 nM、267 nM、268 nM、269 nM、270 nM、271 nM、272 nM、273 nM、274 nM、275 nM、276 nM、277 nM、278 nM、279 nM、280 nM、281 nM、282 nM、283 nM、284nM、285 nM、286 nM、287 nM、288 nM、289 nM、290 nM、291 nM、292 nM、293 nM、294 nM、295 nM、296 nM、297 nM、298 nM、299 nM、300 nM、301 nM、302 nM、303nM、304 nM、305 nM、306 nM、307 nM、308 nM、309 nM、310 nM、311 nM、312 nM、313 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在各种实施例中，培养基中盐的总浓度为约10nM至约100μM。
在各种实施例中，培养基中盐的总浓度为约5nM至约50μM。
在各种实施例中，培养基中盐的总浓度为约20μM至约25μM。
在其他实施例中，培养基补充有足以提供培养基中盐的总浓度的量的盐。
在一个实施例中，盐是硫酸铜并且宿主细胞是昆虫细胞，其中培养基中盐的总浓度为约10nM至约100μM。
在各种实施例中，过渡金属或盐的有效量增加VP1蛋白的掺入，其中VP1蛋白占衣壳蛋白的2％、3％、4％、5％、6％、7％、8％、9％、10％、11％、12％、13％、14％、15％、16％、17％、18％、19％、20％、21％、22％、23％、24％、25％、26％、27％、28％、29％、30％、31％、32％、33％、34％、35％、36％、37％、38％、39％、40％、41％、42％、43％、44％、45％、46％、47％、48％、49％或50％。在其他实施例中，VP1蛋白百分比是介于上文提供的任何两个百分比之间的范围。
在各种实施例中，过渡金属或盐的有效量增加掺入，其中衣壳中VP1蛋白的平均数量为1.5、2、2.5、3、3.5、4、4.5、5、5.5、6、6.5、7、7.5、8、8.5、9、9.5、10、10.5、11、11.5、12、12.5、13、13.5、14、14.5、15、15.5、16、16.5、17、17.5、18、18.5、19、19.5、20、20.5、21、21.5、22、22.5、23、23.5、24、24.5、25、25.5、26、26.5、27、27.5、28、28.5、29、29.5、30、30.5、31、31.5、32、32.5、33、33.5、34、34.5、35、35.5、36、36.5、37、37.5、38、38.5、39、39.5或40个。在其他实施例中，衣壳中VP1蛋白的平均数量是介于上文提供的两个值之间的范围。在其他实施例中，每个rAAV衣壳的VP1蛋白为至少5个、至少6个、至少7个、至少8个、至少9个、至少10个、5至10个、6至9个、5个、6个、7个、8个、9个或10个。
在其他实施例中，该方法进一步包括例如从培养基中分离由宿主细胞产生的AAV病毒颗粒。
半胱氨酸蛋白酶的抑制和半胱氨酸蛋白酶抑制剂
尽管不希望受理论束缚，但据信半胱氨酸蛋白酶的抑制增加VP1在由在培养基中培养的宿主产生的AAV病毒颗粒的衣壳中的掺入。
因此，在其他方面，本发明提供了一种用于制备腺相关病毒(AAV)病毒颗粒的方法，该方法包括在包含有效量的半胱氨酸蛋白酶抑制剂的培养基中培养能够产生AAV病毒颗粒的宿主细胞的步骤，其中AAV病毒颗粒包含AAV衣壳，该衣壳包含VP1蛋白。
在一些实施例中，宿主细胞和AAV病毒颗粒如本文所公开的。
在一个实施例中，该方法包括通过调制调制抑制剂的表达或活性的蛋白质或因子来直接或间接调制宿主细胞中抑制剂的表达或活性，由此提供足以抑制或降低半胱氨酸蛋白酶的活性的抑制剂有效量，由此与掺入在由在除了没有有效量的抑制剂外类似或基本上类似的培养条件下培养的宿主细胞产生的AAV病毒颗粒的衣壳中的VP1的量相比，增加了VP1在由宿主细胞产生的AAV病毒颗粒的衣壳中的掺入。
在另一个实施例中，调制宿主细胞中抑制剂的表达或活性包括将抑制剂添加到培养基。
在一些实施例中，抑制剂可以抑制菠萝蛋白酶、钙蛋白酶、半胱天冬酶、组织蛋白酶(例如，B、H和/或L)、木瓜凝乳蛋白酶、无花果蛋白酶或木瓜蛋白酶的半胱氨酸蛋白酶活性。
在其他实施例中，抑制剂包含亮抑酶肽(Ac-Leu-Leu-Arg-CHO)、E-64(L-反式-3-羧基环氧乙烷-2-羰基-L-亮氨酰基精胺)、L-反式环氧琥珀酰基-L-亮氨酰基氨基-3-甲基-丁烷、抗痛素二盐酸盐、糜抑素微生物、N-乙基马来酰亚胺、α2-巨球蛋白或苯甲磺酰氟。
在各种实施例中，半胱氨酸蛋白酶抑制剂的有效量增加VP1蛋白的掺入，其中VP1蛋白占衣壳蛋白的2％、3％、4％、5％、6％、7％、8％、9％、10％、11％、12％、13％、14％、15％、16％、17％、18％、19％、20％、21％、22％、23％、24％、25％、26％、27％、28％、29％、30％、31％、32％、33％、34％、35％、36％、37％、38％、39％、40％、41％、42％、43％、44％、45％、46％、47％、48％、49％或50％。在其他实施例中，VP1蛋白百分比是介于上文提供的任何两个百分比之间的范围。
在各种实施例中，半胱氨酸蛋白酶抑制剂的有效量增加掺入，其中衣壳中VP1蛋白的平均数量为1.5、2、2.5、3、3.5、4、4.5、5、5.5、6、6.5、7、7.5、8、8.5、9、9.5、10、10.5、11、11.5、12、12.5、13、13.5、14、14.5、15、15.5、16、16.5、17、17.5、18、18.5、19、19.5、20、20.5、21、21.5、22、22.5、23、23.5、24、24.5、25、25.5、26、26.5、27、27.5、28、28.5、29、29.5、30、30.5、31、31.5、32、32.5、33、33.5、34、34.5、35、35.5、36、36.5、37、37.5、38、38.5、39、39.5或40个。在其他实施例中，衣壳中VP1蛋白的平均数量是介于上文提供的两个值之间的范围。在其他实施例中，每个rAAV衣壳的VP1蛋白为至少5个、至少6个、至少7个、至少8个、至少9个、至少10个、5至10个、6至9个、5个、6个、7个、8个、9个或10个。
杆状病毒病毒粒子
在一些实施例中，采用杆状病毒系统。
杆状病毒是节肢动物的有包膜DNA病毒，其中的两个成员因在细胞培养物中产生重组蛋白而众所周知。杆状病毒具有环状双链基因组(80-200kbp)，该环状双链基因组可以被工程化以允许将大基因组内容递送到特异性细胞。用作载体的病毒通常是苜蓿银纹夜蛾多衣壳核多角体病毒(AcMNPV)或桑蚕(Bm)NPV)。
杆状病毒通常用于感染昆虫细胞以用于表达重组蛋白。具体地，可以实现异源基因在昆虫中的表达，如例如以下中描述的：美国专利号4,745,051；Friesen等人(1986)；EP127,839；以及EP 155,476。可以用于蛋白质产生的许多杆状病毒株和变体以及对应的允许昆虫宿主细胞是本领域已知的。
例如，商业上可获得的系统(伊利诺伊州罗克福德的赛默飞世尔科技公司(Thermo Fisher Scientific，Rockford，IL))(目录号10359016)包括用于重组蛋白表达的表达载体。pFastBacTM 1载体(伊利诺伊州罗克福德的赛默飞世尔科技公司)具有用于高水平蛋白质表达的强多角体蛋白启动子和用于简化的克隆的大型多克隆位点。pFastBacTM Dual(伊利诺伊州罗克福德的赛默飞世尔科技公司)是单一载体，其在单一载体中以两种强启动子即多角体启动子和p10启动子为特征以用于在昆虫细胞中同时表达两种蛋白质。
简而言之，通过说明的方式，杆状病毒系统诸如依赖于通过大肠杆菌中的位点特异性转座而不是昆虫细胞中的同源重组来生成重组杆状病毒。例如，可以将所关注的基因克隆到pFastBacTM载体中并转化为DH10BacTM感受态大肠杆菌(伊利诺伊州罗克福德的赛默飞世尔科技公司)。DH10BacTM含有具有lacZ-微小attTn7融合体的亲本杆粒。在由辅助质粒提供的转座蛋白的存在下，在pFastBacTM载体元件与亲本杆粒之间进行转座。当转座成功时，表达盒破坏lacZ基因，并且新的表达杆粒可以可视化为白色细菌菌落。可以将新的表达杆粒分离并用于使用合适的转染试剂来转染例如Sf9或Sf21细胞。转染试剂的实例包括脂质体、阳离子聚合物(例如，聚(乙撑亚胺))、阳离子肽(例如，聚-L-赖氨酸)、Lipofectin(赛默飞世尔科技公司)、Cellfectin(赛默飞世尔科技公司)、Cellfectin II(赛默飞世尔科技公司)、Expifectamine Sf(赛默飞世尔科技公司)、/>(Mirus Bio公司(Mirus Bio))、Insect/>(Biontex公司(Biontex))、以及美国专利号5,674,908、5,834,439和6,110,916中公开的转染试剂，所有美国专利均通过引用整体并入本文。在培养适当的时间量之后，可以分离重组杆状病毒。重组杆状病毒可以用于感染细胞以产生AAV病毒颗粒和/或表达所关注的基因。
在一个实施例中，本发明的杆状病毒系统包含杆状病毒转染的细胞，该细胞维持在使得杆状病毒病毒粒子(BV)产生的条件下。然后收集这些产生的杆状病毒病毒粒子以供其随后用于感染宿主细胞。
在一些实施例中，用于制备腺相关病毒(AAV)病毒颗粒的方法包括
在包含有效量的盐的培养基中培养能够产生AAV病毒颗粒的宿主细胞，其中AAV病毒颗粒包含AAV衣壳，该衣壳包含VP1蛋白，
其中宿主细胞包含：第一核酸载体，其包含侧接包含一个或多个转基因的5'和3'AAV反向末端重复序列，该转基因包含一个或多个异源基因，该第一核酸载体与控制一个或多个异源基因在宿主中的表达的调节序列细胞可操作地连接：以及第二核酸载体，其包含AAV rep和cap核酸序列，
其中所述cap核酸序列编码AAV衣壳，
其中AAV颗粒用AAV衣壳假型化，并且
其中第一核酸载体通过由包含第一核酸载体的杆状病毒感染宿主细胞来引入到宿主细胞中。
在其他实施例中，通过由包含第一核酸载体的第一杆状病毒和包含第二核酸载体的第二杆状病毒感染宿主细胞来将第一核酸载体和第二核酸载体引入到宿主细胞中。
在一些实施例中，增加转染或感染培养基和生产者培养基中的盐浓度，使得转染或感染培养基和生产者培养基中的盐浓度高于预产生培养基中的盐浓度。
在另一个实施例中，该方法进一步包括从宿主细胞和/或宿主细胞的上清液中分离、纯化或以其他方式回收AAV病毒颗粒。
AAV病毒颗粒的纯化
在其他实施例中，可以使用多种常规纯化方法诸如柱色谱法、CsCl梯度等从宿主细胞中纯化AAV病毒颗粒。例如，可以使用多个柱纯化步骤，诸如在阴离子交换柱、亲和柱和/或阳离子交换柱上纯化。参见例如国际公开号WO 02/12455。进一步地，如果采用感染来表达附属功能，则可以使用已知的方法来灭活残留的辅助病毒。例如，可以通过加热至大约60℃的温度例如20分钟或更久来灭活腺病毒。该治疗仅有效地灭活辅助病毒，因为AAV是极强地热稳定的，而辅助腺病毒是热不稳定的。
在一个实施例中，然后例如使用柱色谱法技术来处理AAV病毒颗粒储备以移除空衣壳。
在另一个实施例中，通过裂解转染的细胞以获得粗细胞裂解物来获得AAV病毒颗粒制剂。然后可以通过本领域众所周知的技术诸如过滤、离心等来澄清粗细胞裂解物以移除细胞碎片，从而得到澄清的细胞裂解物。然后可以在非分离条件下将可以含有AAV病毒颗粒和AAV空衣壳两者的粗细胞裂解物或澄清的细胞裂解物应用于第一阳离子交换基质，其中第一阳离子交换柱用于进一步从细胞裂解物制剂中存在的细胞和其他组分中分离AAV病毒颗粒和AAV空衣壳。用于执行细胞裂解物的初始纯化的方法是已知的。一种代表性方法描述于美国专利第6,593,123号，其以全文引用的方式并入本文中。
药物调配物
在其他方面，本发明涉及可用于施用于受试者的本发明的AAV病毒颗粒的药物调配物。
在一个实施例中，本发明的药物调配物是包含本文公开的AAV病毒颗粒的液体调配物，其中调配物中AAV病毒颗粒的浓度可以广泛地变化。AAV病毒颗粒和组合物可以按剂量单位形式调配，以便于施用和剂量的均匀性。如本文所使用的，剂量单位形式是指适合作为用于待治疗的个体的单位剂量的物理上离散的单位；每个单位含有被计算为与所需的药物载体相关联地产生所需治疗效果的预定量的活性化合物。剂量单位形式取决于产生所需效果所需的AAV病毒颗粒的量。所需的量可以按单个剂量调配或可以按多个剂量单位调配。可以将剂量调整至合适的AAV病毒颗粒浓度、任选地与一种或多种其他药剂组合并包装以供使用。
在另一个实施例中，药物组合物将包含足够的遗传物质以提供预防或治疗有效量，即足以预防、减轻或改善所讨论的疾病状态的症状的量或足以赋予所需益处的量。
在其他实施例中，本发明的含有AAV病毒颗粒的药物调配物包含一种或多种药学上可接受的赋形剂以为调配物提供有利于储存和/或施用于受试者的特性。在某些实施例中，本发明的药物调配物能够在<65℃储存至少2周、优选地至少4周、更优选地至少6周并且还更优选地至少约8周的时间段，而没有可检测到的稳定性变化。在这方面，术语“稳定”意味着，调配物中存在的AAV病毒颗粒在储存期间基本上保留其物理稳定性、化学稳定性和/或生物活性。在本发明的其他实施例中，药物调配物中存在的AAV病毒颗粒在-65℃储存确定的时间段期间在受试者中保留其生物活性的至少约80％，更优选地在受试者中保留其生物活性的至少约85％、90％、95％、98％或99％。
在一些实施例中，磷酸氢二钠的浓度为约0.1mg/ml至约3mg/ml、约0.5mg/ml至约2.5mg/ml、约1mg/ml至约2mg/ml或约1.4mg/ml至约1.6mg/ml。在特别优选的实施例中，本发明的AAV病毒颗粒调配物包含约1.42mg/ml的磷酸氢二钠(干燥的)。
在其他实施例中，可用于本发明的AAV病毒颗粒调配物中的另一种缓冲剂是磷酸二氢钠一水合物，其在一些实施例中以约0.1mg/ml至约3mg/ml、约0.5mg/ml至约2.5mg/ml、约1mg/ml至约2mg/ml或约1.3mg/ml至约1.5mg/ml的浓度使用。在一个实施例中，本发明的AAV病毒颗粒调配物包含约1.38mg/ml的磷酸二氢钠一水合物。在另一个实施例中，本发明的AAV病毒颗粒调配物包含约1.42mg/ml的磷酸氢二钠和约1.38mg/ml的磷酸二氢钠一水合物。
在一个实施例中，本发明的AAV病毒颗粒调配物可以包含优选地约1mg/ml至约20mg/ml，例如约1mg/ml至约10mg/ml、约5mg/ml至约15mg/ml或约8mg/ml至约20mg/ml的浓度的一种或多种等渗剂，诸如氯化钠。在另一个实施例中，本发明的调配物包含约8.18mg/ml氯化钠。本领域已知的其他缓冲剂和等渗剂是合适的并且可以常规地采用以用于本公开的调配物中。
在另一个实施例中，本发明的AAV病毒颗粒调配物可以包含一种或多种填充剂。示例性填充剂包括但不限于甘露醇、蔗糖、葡聚糖、乳糖、海藻糖和聚维酮(PVP K24)。在某些优选的实施例中，本发明的调配物包含甘露醇，其可以以约5mg/ml至约40mg/ml、或约10mg/ml至约30mg/ml、或约15mg/ml至约25mg/ml的量存在。在特别优选的实施例中，甘露醇以约20mg/ml的浓度存在。
在一些实施例中，本发明的AAV病毒颗粒调配物可以包含一种或多种表面活性剂，该表面活性剂可以是非离子表面活性剂。
示例性表面活性剂包括但不限于离子表面活性剂、非离子表面活性剂及其组合。例如，表面活性剂可以是但不限于TWEEN 80(也称为聚山梨醇酯80或其化学名称聚氧乙烯脱水山梨糖醇单油酸酯)、十二烷基硫酸钠、硬脂酸钠、十二烷基硫酸铵、TRITON AG 98(罗讷-普朗克公司(Rhone-Poulenc))、泊洛沙姆407、泊洛沙姆188等及其组合。
在一些实施例中，本发明的调配物包含泊洛沙姆188，其可以以约0.1mg/ml至约4mg/ml、或约0.5mg/ml至约3mg/ml、约1mg/ml至约3mg/ml、约1.5mg/ml至约2.5mg/ml、或约1.8mg/ml至约2.2mg/ml的浓度存在。在特别优选的实施例中，泊洛沙姆188以约2.0mg/ml的浓度存在。
在其他实施例中，本发明的药物调配物包含以液体溶液调配的AAV病毒颗粒，该液体溶液包含约1.42mg/ml的磷酸氢二钠、约1.38mg/ml的磷酸二氢钠一水合物、约8.18mg/ml氯化钠、约20mg/ml甘露醇和约2mg/ml泊洛沙姆188。
在一些实施例中，本公开的含有AAV病毒颗粒的调配物是稳定的并且可以储存延长的时间段而没有不可接受的质量、效力或纯度变化。
在一个实施例中，调配物在约5℃(例如，2℃至8℃)的温度下稳定至少1个月，例如至少1个月、至少3个月、至少6个月、至少12个月、至少18个月、至少24个月或更久。
在另一个实施例中，调配物在小于或等于约-20℃的温度下稳定至少6个月，例如至少6个月、至少12个月、至少18个月、至少24个月、至少36个月或更久。
在一些实施例中，调配物在小于或等于约-40℃的温度下稳定至少6个月，例如至少6个月、至少12个月、至少18个月、至少24个月、至少36个月或更久。
在其他实施例中，调配物在小于或等于约-60℃的温度下稳定至少6个月，例如至少6个月、至少12个月、至少18个月、至少24个月、至少36个月或更久。
转导/治疗
一方面，本发明提供了本发明的AAV病毒颗粒用于高效地转导所关注的细胞、组织和/或器官和/或用于基因疗法的用途。
在一个实施例中，本发明提供了一种用于转导所关注的细胞、组织和/或器官的方法，包括将包含有效量的本发明的AAV病毒颗粒的组合物引入到细胞中。
在一些实施例中，本发明的AAV病毒颗粒用于转导受试者的所关注的细胞、组织和/或器官。
在其他实施例中，提供了一种用于转导所关注的细胞、组织和/或器官的方法，包括引入到细胞中，该方法包括包含有效量的本发明的AAV病毒颗粒的组合物。
在一个实施例中，提供了用于对受试者进行预防性或治疗性治疗的方法。
在另一个实施例中，受试者需要预防性或治疗性治疗。
在一些实施例中，受试者包含病状或疾病，其中受试者需要治疗所述病状或疾病。
在其他实施例中，受试者是哺乳动物。
在一个实施例中，受试者是非啮齿类哺乳动物。
在另一个实施例中，受试者是灵长类动物。
在一些实施例中，受试者是人。
在其他实施例中，受试者是牲畜。
在一个实施例中，受试者是马、绵羊、山羊、猪、狗或猫。
在另一个实施例中，本发明的AAV病毒颗粒可以通过多种已知的施用技术施用于受试者。
在一些实施例中，AAV病毒颗粒通过静脉内注射作为单次推注或在延长的时间段内施用，该延长的时间段可以是至少约1、5、10、15、30、45、60、75、90、120、150、180、210或240分钟或更久。
在一个实施例中，由所述AAV病毒颗粒转导包含受试者的脑脊液(CSF)的细胞(例如，室管膜细胞)。在一些实施例中，用AAV病毒颗粒转导的细胞(例如，室管膜细胞)表达转基因并将转基因分泌到所述哺乳动物的CSF中。
在另一个实施例中，AAV病毒颗粒的施用包括施用于受试者的小脑延髓池、心室内空间、脑室、蛛网膜下腔、鞘内空间和/或室管膜。
在其他实施例中，AAV病毒颗粒的施用包括施用于所述受试者的脑脊液(CSF)。
在一些实施例中，AAV病毒颗粒的施用包括使所述受试者的室管膜细胞与AAV病毒颗粒接触。
在一个实施例中，AAV病毒颗粒的施用包括使所述受试者的软膜细胞、内皮细胞或脑膜细胞与所述AAV病毒颗粒接触。
在另一个实施例中，AAV病毒颗粒的施用包括将AAV病毒颗粒注射到所述受试者的脑或脊髓的组织或液体中。
在一些实施例中，AAV病毒颗粒的施用包括将AAV病毒颗粒注射到所述受试者的脑脊液中。
试剂盒
另一方面，本发明提供了用于与本文描述的方法和组合物一起使用的试剂盒。试剂盒中可以提供组合物和病毒调配物。试剂盒还可以包含合适的容器以及任选地一种或多种附加药剂。在一些实施例中，容器是小瓶、试管、烧瓶、瓶子、注射器和/或其他容器。在其他实施例中，试剂盒包含AAV病毒颗粒、药学上可接受的载体和供其使用(例如，用于指导AAV病毒颗粒的施用)的说明材料。
本发明将在以下实例中进一步描述，这些实例并不限制权利要求中描述的本发明的范围。
实例
实例1
用硫酸铜来补充培养基提高了Bba41衣壳的VP1表达并提高了用Bba41衣壳假型化的AAV颗粒的感染性
发明内容
使用基于昆虫细胞的rAAV产生平台来产生rAAV衣壳。该衣壳的产物质量和效力分析示出与HEK293系统中产生的rAAV的VP1％相比低得多的衣壳蛋白的VP1％。因此，与HEK293细胞中产生的rAAV衣壳相比，昆虫细胞中产生的rAAV衣壳示出显著更低的效力，如通过对由包装的AAV转基因进行的荧光素酶表达进行定量的转导测定来测量的。
为了提高基于昆虫细胞的rAAV产生平台中产生的衣壳的效力，评价将硫酸铜(CuSO4)补充到昆虫细胞培养物的影响。测试一系列铜浓度(0μM–50μM)以研究对AAV产生的影响。对在铜的存在或不存在下产生的rAAV批次进行纯化和分析。鉴定出，当与在没有铜补充的情况下产生的AAV衣壳相比时，在产生AAV衣壳的昆虫细胞中补充铜显著提高了衣壳效力。例如，当与没有任何铜补充的昆虫细胞系统中产生的对照AAV衣壳相比时，AAV衣壳效力增加了约10倍。AAV衣壳蛋白的分析示出与没有铜的对照产生批次的VP1％相比，铜补充的AAV产生批次中产生的AAV的VP1％显著增加。在一个实例中，与在没有铜补充的情况下产生的对照AAV衣壳相比，观察到AAV衣壳的VP1％增加了几乎两倍。用铜补充的昆虫细胞产生平台产生的AAV衣壳的比较示出与HEK293产生平台中产生的AAV衣壳类似的效力。
材料和方法
细胞培养：在28摄氏度(℃)下培养Sf9昆虫细胞(赛默飞世尔科技公司)。
使悬浮Expi293细胞(赛默飞世尔科技公司)在设定为37℃的摇床培养箱中生长。
使用于转导测定的贴壁HEK293T细胞在37℃静态培养箱中生长。
使用Vi-Cell计数器(贝克曼库尔特公司(Beckman Coulter))来测量细胞密度、活力和平均细胞直径。使用Nova Flex自动细胞计数器(诺瓦医疗公司(Nova Medical))来测量在昆虫和Expi293细胞中的AAV产生期间细胞培养基中代谢物和气体的细胞培养物浓度。
AAV产生和纯化：为了产生用Bba41衣壳(SEQ ID NO:15)假型化的rAAV，使用基于昆虫细胞的产生过程来在Sf9衍生细胞系中产生绿色荧光蛋白-荧光素酶(GFP-Luc)rAAV。通过用携带绿色荧光蛋白(GFP)和荧光素酶(Luc)转基因的重组杆状病毒(rBV)共感染Sf9衍生细胞并提供AAV Rep和Cap(Bba41)基因来产生GFP-Luc rAAV颗粒。由杆粒产生rBV，该杆粒是当转染到昆虫细胞中时可以繁殖rBV的穿梭载体。
对于昆虫细胞中的AAV产生，使用Bac-to-Bac杆状病毒表达系统(赛默飞世尔科技公司)来产生源自苜蓿银纹夜蛾核型多水解病毒(AcNPV)的rBV。AAV产生过程包括分批细胞培养以及收获用rBV感染的昆虫细胞。
对于HEK293细胞中的AAV产生，使用expifectamine转染试剂来转染含有rAAV产生所需的Rep、Cap、Ad辅助和所关注的基因(GOI)元件的质粒。然后从收获物中纯化rAAV。
重组AAV衣壳的VP1％分析：通过以等于每个泳道2E+11个衣壳的量上样两步纯化的AAV样品，在SDS-PAGE上分析重组AAV衣壳的VP1％。经由Chemidoc成像仪将纯蓝色考马斯(赛默飞世尔科技公司)染色的凝胶可视化，并且使用实验室图像软件套件来执行解析的带的密度测定分析。
体外转导测定：在目标感染复数下用纯化的AAV来感染平板接种在96孔板中的贴壁HEK293T细胞(ATCC)。将纯化的rAAV在平板接种培养基中稀释以达到最终体积。将病毒悬浮液添加到平板接种在96孔板中的平板接种HEK293T细胞中并孵育。将One-Glo荧光素酶测定试剂(普洛麦格公司(Promega))添加到细胞，并且在GloMax读取器(普洛麦格公司)中读取板。根据由来自GloMax板读取器的输出数据计算的相对发光单位(RLU)来测量转导效率。
通过AAV衣壳来分析DNA包装：按照标准方案使用0.8％碱性琼脂糖凝胶来解析包装的DNA。为每个样品使用如通过液滴数字聚合酶链反应方法测量的1E+11vg。使用SYBRGold染料(赛默飞世尔科技公司)来对凝胶进行染色。在Chemidoc的Epiblue透照仪设置上对染色的凝胶进行成像。
结果和讨论
产生AAV的铜补充的昆虫细胞培养物导致AAV衣壳的转导活性显著提高：以0μM、5μM或50μM浓度将铜添加到产生AAV衣壳的昆虫细胞培养物中。对从各种铜补充的昆虫培养物中产生的rAAV进行分离、纯化和分析。使用转导测定来测量纯化的AAV的效力。0μM硫酸铜补充的昆虫细胞培养物产生滴度为3.4x 10E11 vg/ml的rAAV。5μM硫酸铜补充的昆虫细胞培养物产生滴度为2.5x 10E11 vg/ml的rAAV。50μM硫酸铜补充的昆虫细胞培养物产生滴度为1.6x 10E11 vg/ml的rAAV。应注意的是，增加的铜浓度产生可接受的rAAV滴度，即使滴度未增加。如图1所示，转导测定示出铜对新型AAV衣壳的效力的剂量依赖性影响。在50μM铜补充的昆虫细胞培养物中产生的AAV衣壳示出比在没有任何铜的情况下产生的AAV高几乎十倍的效力，如通过转导测定中的发光单位(LU)测量的。类似地，在5μM铜补充的昆虫细胞培养物中产生的AAV衣壳示出比在没有任何铜的情况下产生的AAV高几乎四倍的效力。
由铜补充的昆虫细胞培养物产生的AAV衣壳显著提高了VP1％：对在铜的存在或不存在下使用昆虫细胞产生过程或HEK293产生过程产生的rAAV进行分离、纯化和分析。图2是在SDS-PAGE凝胶(4％-12％bis Tris凝胶、MOPS运行缓冲液)上解析在各种铜补充条件下最终量为每个泳道2x 10E+11个衣壳的纯化衣壳的十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)。SDS-PAGE凝胶中使用的分子量标准是Seeblue plus。凝胶的泳道1来自补充有0μM铜的Sf9细胞中产生的衣壳，其中VP1蛋白占衣壳的总VP1-3蛋白浓度的2.3％。凝胶的泳道2来自补充有5μM铜的Sf9细胞中产生的衣壳，其中VP1蛋白占衣壳的总VP1-3蛋白浓度的2.9％。凝胶的泳道3来自补充有50μM铜的Sf9细胞中产生的衣壳，其中VP1蛋白占衣壳的总VP1-3蛋白浓度的4.5％。凝胶的泳道4来自补充有0μM铜的HEK293细胞中产生的衣壳，其中VP1蛋白占衣壳的总VP1-3蛋白浓度的6％。凝胶的泳道5来自补充有5μM铜的HEK293细胞中产生的衣壳，其中VP1蛋白占衣壳的总VP1-3蛋白浓度的5.9％。凝胶的泳道6来自补充有50μM铜的HEK293细胞中产生的衣壳，其中VP1蛋白占衣壳的总VP1-3蛋白浓度的5.7％。通过由考马斯蓝染色法对凝胶进行染色来将VP1、VP2和VP3衣壳蛋白可视化。如通过密度测定分析来测量的，通过在Sf9细胞培养物中补充50μM铜而产生的衣壳的VP1％比在没有任何铜补充的情况下产生的衣壳高几乎两倍。在单独的实验中，还在SDS PAGE凝胶上解析在293产生过程中通过补充铜而产生的rAAV。然而，在补充有50μM铜的HEK293细胞中产生的rAAV中未观察到VP1％有显著提高。出乎意料的是，铜补充将会增加昆虫细胞中产生的rAAV的VP1％并维持与在没有铜补充的情况下产生rAAV的昆虫细胞的滴度类似的滴度。已知VP1％在AAV衣壳的转导效率中发挥直接作用，并且因此，AAV衣壳的更高效力归因于由于昆虫细胞培养物中的铜补充而导致的这些衣壳中提高VP1％。应注意的是，泳道2和3具有与泳道4-6类似的带型。这表明铜补充似乎不会影响VP1蛋白的带型。不希望受理论束缚，据信铜补充改变昆虫细胞中产生的衣壳的组成VP蛋白，使得VP蛋白谱与HEK293细胞中产生的衣壳类似。
由铜补充的昆虫细胞培养物产生的AAV衣壳示出与由HEK293产生平台产生的AAV衣壳相比类似的效力：对昆虫细胞或HEK293细胞中产生的纯化的AAV衣壳执行转导测定。如图3所示，由50μM铜补充的昆虫细胞培养物产生的AAV衣壳具有与摇床瓶或生物反应器中由HEK293细胞产生的AAV衣壳类似的效力。虽然具有铜补充的昆虫细胞培养物中产生的衣壳具有较差的效力，但昆虫细胞培养物的铜补充生成了具有与HEK293细胞中产生的AAV衣壳类似的效力的AAV衣壳。出乎意料的是，铜补充将会增加昆虫细胞中产生的rAAV的效力并维持与在没有铜补充的情况下产生rAAV的昆虫细胞的滴度类似的滴度。因此，铜补充增加rAAV颗粒的治疗性有效性。
如通过碱性琼脂糖凝胶示出的在铜补充的昆虫细胞产生平台和293产生平台的情况下AAV衣壳中的包装的转基因DNA谱：如先前描述的在碱性琼脂糖凝胶上解析由昆虫细胞产生平台或293产生平台产生的纯化的AAV衣壳。图4是采用SYBR Gold的0.8％碱性琼脂糖凝胶，其中将1x 10E11 vg上样到凝胶上。图4的泳道1和12是Ladder Promega G5711分子量标准品。泳道2来自从2x 1L Sf细胞摇瓶中产生的1.74x 10E14rAAV滴度，rAAV具有>4千碱基对(Kb)的vg大小。泳道3来自从2x 1L Sf细胞摇瓶中产生的4.2x 10E14 rAAV滴度，rAAV具有>4Kb的vg大小。泳道4来自从3L HEK293细胞生物反应器中产生的6.5x 10E13 rAAV滴度，rAAV具有>4Kb的vg大小。泳道5来自从补充有0μM硫酸铜的Sf9细胞中产生的7.8x 10E13rAAV滴度，rAAV具有>4Kb的vg大小。泳道6来自从补充有5μM硫酸铜的Sf9细胞中产生的2.2x10E13 rAAV滴度，rAAV具有>4Kb的vg大小。泳道7来自从补充有50μM硫酸铜的Sf9细胞中产生的1.5x 10E13 rAAV滴度，rAAV具有>4Kb的vg大小。泳道8来自从补充有0μM硫酸铜的HEK293细胞中产生的1.0x 10E13 rAAV滴度，rAAV具有>4Kb的vg大小。泳道9来自从补充有50μM硫酸铜的HEK293细胞中产生的1.0x 10E13 rAAV滴度，rAAV具有>4Kb的vg大小。泳道10来自从补充有50μM硫酸铜的HEK293细胞中产生的6.6x 10E12 rAAV滴度，rAAV具有>4Kb的vg大小。泳道10来自6.5x 10E14 rAAV滴度，rAAV具有>4Kb的vg大小。如图4所示，在有铜(泳道6和7)和没有铜(泳道5)补充的昆虫细胞产生的AAV衣壳中，包装的DNA的DNA谱均仍然非常类似。
实例2
用硫酸铜来补充培养基提高了AAV5衣壳的VP1表达并提高了用AAV5衣壳假型化的AAV颗粒的感染性
摇瓶中的AAV5产生：对于昆虫细胞中的AAV产生，按照制造商的方案(赛默飞世尔科技公司)使用Bac-to-Bac杆状病毒表达系统来产生源自苜蓿银纹夜蛾核型多水解病毒(AcNPV)的重组杆状病毒(rBV)。AAV产生过程包括在摇瓶中分批细胞培养以及收获已经用具有转基因多核苷酸序列(GOI)的rBV和具有编码Rep和Cap(病毒载体复制和衣壳蛋白)的序列的rBV共感染的昆虫细胞，之后使用AVB琼脂糖凝胶(sepharose)亲和捕获进行纯化。在该实例中，编码Cap的序列特别编码AAV5血清型衣壳(NCBI参考序列号YP_068409.1)。在产生过程期间，将硫酸铜(CuSO4)添加到细胞培养物。测试不同硫酸铜浓度(5μM、25μM和45μM)。包括两个烧瓶作为对照(0μM硫酸铜)。
使用反相高效液相色谱法(RP-HPLC)来评估在不同硫酸铜浓度的情况下产生的rAAV颗粒的衣壳的VP1水平。RP-HPLC方法在离子对剂三氟乙酸的存在下采用C3柱和乙腈梯度。将rAAV颗粒注射到柱，在该柱中，rAAV颗粒被解离并且三种类型的衣壳蛋白(VP1、VP2和VP3)被分离。使用UV 214nm峰下的面积来对三种病毒外壳蛋白的相对峰面积百分比进行定量，并报告VP1％。
如图5所示，将铜添加到细胞培养物增加了VP1到rAAV颗粒的衣壳中的掺入。特别地，当将5μM、25μM和45μM硫酸铜添加到细胞培养物时，看到衣壳中的VP1增加。在5μM硫酸铜的情况下产生的AAV5衣壳的VP1％的范围为～4.25％至>4.5％。在25μM硫酸铜的情况下产生的AAV5衣壳的VP1％≥5％。在45μM硫酸铜的情况下产生的AAV5衣壳的VP1％≥4.5％。在没有硫酸铜的情况下产生的AAV5衣壳的VP1％为4％。因此，不同的铜浓度增加了AAV％衣壳的VP1含量。图5还示出了与rAAV颗粒的对照产生相比，～30μM浓度的硫酸铜能够提高衣壳中的VP1含量。
为了测试对rAAV颗粒的感染性的影响，用rAAV颗粒来感染HepG2细胞(ATCC)。为了对被感染的HepG2细胞中所关注的基因的蛋白质表达进行定量，收获来自细胞培养物的上清液，并且使被感染的HepG2细胞裂解。经由酶联免疫吸附测定(ELISA)来分析蛋白质样品。将蛋白质样品与固定到孔的表面上的捕获抗体一起孵育。捕获抗体特异性地结合由GOI编码的蛋白质。由GOI编码的蛋白质与捕获抗体结合之后，将由GOI编码的蛋白质靶向捕获抗体的检测抗体添加到孔。将捕获抗体与检测元件诸如辣根过氧化物酶缀合，以用于捕获蛋白的定量。使用蛋白质标准曲线来对由GOI编码的蛋白质进行定量。
如图6所示，将铜添加到细胞培养物增强了rAAV颗粒的感染性。特别地，相对于在没有铜的情况下产生的AAV5衣壳的感染性，在25μM硫酸铜的情况下产生的AAV5衣壳具有124％、106％和117％的感染性/相对效力。因此，铜补充增加rAAV颗粒的治疗性有效性。
在生物反应器中产生的AAV5：为了示出铜对大规模rAAV产生的影响，在生物反应器中进行rAAV产生。特别地，AAV产生过程包括在3L和2000L生物反应器中分批细胞培养以及收获已经用具有转基因多核苷酸序列(所关注的基因(GOI))的rBV和具有编码Rep和Cap(病毒载体复制和衣壳蛋白)的序列的rBV共感染的昆虫细胞，之后使用AVB琼脂糖凝胶亲和捕获和/或离子交换色谱法进行纯化。在该实例中，编码Cap的序列还编码AAV5血清型衣壳。在产生过程期间，将30μM硫酸铜添加到细胞培养物。使用RP-HPLC来评估rAAV颗粒的衣壳的VP1水平。
图7示出了rAAV颗粒的RP-HPLC分析，其中在扩大规模的rAAV产生中添加硫酸铜增加了rAAV颗粒的衣壳的VP1水平。在30μM硫酸铜的情况下产生的AAV5衣壳的VP1％为6.16％，而在没有硫酸铜的情况下产生的AAV5衣壳的VP1％为5.25％。图8还示出了在扩大规模的rAAV产生中添加硫酸铜增加了rAAV颗粒的效力。特别地，相对于在没有铜的情况下产生的AAV5衣壳的感染性，在30μM硫酸铜的情况下产生的AAV5衣壳具有147％的感染性/相对效力。对于图7和图8，通过AVB琼脂糖凝胶亲和捕获和离子交换色谱法来纯化rAAV颗粒。
图9示出了在摇瓶、3L生物反应器和2000L生物反应器中产生的rAAV颗粒的RP-HPLC分析。在#1产生中，与没有铜的摇瓶培养物中产生的rAAV颗粒相比，在补充有30μM硫酸铜的3L和2000L生物反应器中产生的rAAV颗粒具有增加的VP1水平。在#2产生中，30μM硫酸铜增加了摇瓶、3L生物反应器和2000L生物反应器产生中的VP1水平。通过AVB琼脂糖凝胶亲和捕获来纯化#1和#2产生中的rAAV颗粒。特别地，如图9所示，添加30μM硫酸铜使VP1％增加至：3L生物反应器产生为4.2％、2000L生物反应器产生为4.1％、摇瓶产生为4.6％、第二3L生物反应器产生为4.8％、以及第二2000L生物反应器产生为4.6％。这些VP1％大于在没有铜的情况下产生的AAV5衣壳的VP1％(摇瓶产生为3.3％和3.2％)。
如图7至图9所示，铜补充提高了衣壳中的VP1含量并增加了rAAV颗粒的治疗性有效性。
实例3
用硫酸铜来补充培养基提高了AAV9衣壳的VP1表达并提高了用AAV9衣壳假型化的AAV颗粒的感染性
对于昆虫细胞中的AAV产生：按照制造商的方案(赛默飞世尔科技公司)使用Bac-to-Bac杆状病毒表达系统来产生源自AcNPV的rBV。AAV产生过程包括在摇瓶中分批细胞培养以及收获已经用具有转基因多核苷酸序列(GOI)的rBV和具有编码Rep和Cap(病毒载体复制和衣壳蛋白)的序列的rBV共感染的昆虫细胞，之后使用免疫色谱法捕获进行纯化。在该实例中，编码Cap的序列特别编码AAV9血清型衣壳(Genbank登录号AAS99264.1)。在产生过程期间，将硫酸铜(CuSO4)添加到细胞培养物。测试不同硫酸铜浓度(0μM、10μM、20μM和30μM)。
使用毛细管电泳(UV 214nm)来评估在不同硫酸铜浓度的情况下产生的rAAV颗粒的衣壳的VP1水平。
图10示出了与rAAV颗粒的对照产生相比，30μM浓度的硫酸铜提高了衣壳中的VP1含量。特别地，30μM硫酸铜将AAV9衣壳的VP1％增加至>6％，而在没有铜的情况下产生的AAV9衣壳具有小于5％的VP1％。
为了测试rAAV颗粒的感染性，在不同感染复数(MOI；病毒与细胞的比率)下用rAAV颗粒来感染HEK293细胞。为了对被感染的HEK293细胞中所关注的基因(例如，荧光素酶)的蛋白质表达进行定量，收获来自细胞培养物的上清液，并且使被感染的HEK293细胞裂解。将荧光素添加到蛋白质样品，并且对相对光单位(RLU)进行定量。如图11所示，与在没有铜的情况下的对照产生培养物相比，将10μM、20μM和30μM硫酸铜添加到细胞培养物增强了rAAV颗粒的感染性。与在没有铜的情况下产生的AAV9衣壳(<5E06至～1E07 RLU)相比，用使用铜补充产生的AAV9衣壳感染的HEK293细胞表现出更高的RLU(>7E06至>3.5E07 RLU)。因此，相比于10μM和20μM铜浓度，30μM的硫酸铜提高了感染性。
如图10和图11所示，铜补充增加了所产生的AAV9衣壳的VP1含量并提高了AAV9衣壳的治疗性有效性。
实例4
硫酸铜补充的培养基中产生的AAV5衣壳的转导/感染性的体内分析
使用补充有硫酸铜(30μM硫酸铜；来自实例2的#1和#2产生)的杆状病毒/Sf9表达系统来产生用AAV5衣壳假型化的rAAV。rAAV的载体基因组包括编码蛋白质(GOI蛋白)的所关注的基因(GOI)。将纯化的载体通过定量聚合酶链反应(qPCR)进行定量并以范围为每千克2e14至2e12个载体基因组(vg/kg)的不同剂量静脉给药到30只Rag2-/-小鼠以及载体对照组(5只Rag2-/-小鼠)中。
给药后52周时，提取肝组织样品以用于随后对载体衍生的DNA和RNA进行定量。按照制造商的方案(凯杰公司(Qiagen))使用DNeasy血液和组织试剂盒和RNeasy Plus Mini试剂盒来从肝组织样品中提取DNA和RNA。提取的DNA和RNA的浓度并在数字液滴聚合酶链反应(ddPCR)分析之前进行稀释。以下中描述了ddPCR的实例：Pasi，K.John，等人"MultiyearFollow-Up of AAV5-hFVIII-SQ Gene Therapy for Hemophilia A."New EnglandJournal of Medicine 382.1(2020):29-40；Regan，John F.，等人"A Rapid MolecularApproach for Chromosomal Phasing."PloS one 10.3(2015):e0118270；以及Furuta-Hanawa，Birei，Teruhide Yamaguchi和Eriko Uchida."Two-Dimensional DropletDigital PCR as a Tool for Titration and Integrity Evaluation of RecombinantAdeno-Associated Viral Vectors"Human gene therapy methods 30.4(2019):127-136。对从给药后52周时收集的肝组织中提取的载体衍生的DNA和mRNA的测量示出了载体DNA和所得到的转录物两者的剂量依赖性增加，其范围为每微克(μg)DNA～1e7至～2e4个拷贝以及每μg RNA～2.3e9至8e6个拷贝。载体对照示出在测定的定量下限以下的DNA水平以及不可检测的mRNA水平。
虽然上文描述了示例性实施例，但是这些实施例并不旨在描述本发明的所有可能形式。相反，说明书中使用的词语是描述性而不是限制性词语，并且应当理解，在不脱离本发明的精神和范围的情况下可以做出各种改变。另外，各种实现实施例的特征可以组合以形成本发明的进一步实施例。
序列表
<110> 生物马林药物股份有限公司 BIOMARIN PHARMACEUTICAL INC.
<120> 用于具有提高的感染性的AAV病毒颗粒的AAV产生系统
<130> 6439-0114PUS1
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<170> PatentIn版本3.5
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Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val Asn
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Ser Val Trp Met Glu Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala
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Lys Ile Pro Glu Thr Gly Ala His Phe His Pro Ser Pro Ala Met Gly
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Pro Val Pro Gly Asn Ile Thr Ser Phe Ser Asp Val Pro Val Ser Ser
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Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro
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Gly Asn Ala Ser Gly Asp Trp His Cys Asp Ser Thr Trp Ser Glu Gly
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Arg Val Thr Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
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Asn His Leu Tyr Leu Arg Ile Gly Thr Thr Ala Asn Ser Asn Thr Tyr
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Lys Glu Val Thr Thr Ser Asn Gly Glu Thr Thr Val Ala Asn Asn Leu
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Thr Ser Thr Val Gln Ile Phe Ala Asp Ser Thr Tyr Glu Leu Pro Tyr
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Val Phe Met Val Pro Gln Tyr Gly Tyr Cys Gly Val Val Thr Gly Glu
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Asn Gln Asn Gln Thr Asp Arg Asn Ala Phe Tyr Cys Leu Glu Tyr Phe
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Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Val Ser Tyr Gln
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Phe Glu Lys Val Pro Phe His Ser Met Tyr Ala His Ser Gln Ser Leu
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Ser Thr Thr Thr Gly Asn Ser Leu Asn Gln Gly Thr Ala Thr Thr Thr
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Leu Pro Gly Ala Cys Ile Lys Gln Gln Lys Phe Ser Lys Asn Ala Ser
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Gln Asn Tyr Lys Ile Pro Ala Ser Gly Gly Asp Ala Leu Leu Lys Tyr
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Asp Thr His Thr Thr Ser Asn Gly Arg Trp Ser Asn Met Ala Pro Gly
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Pro Pro Met Ala Thr Ala Gly Ala Gly Asp Ser Asp Phe Ser Asn Ser
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Ser Asn Asn Leu Leu Phe Thr Ser Glu Glu Glu Ile Ala Thr Thr Asn
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Val Gln Phe Thr Ser Asn Tyr Gly Thr Gln Asn Ser Met Leu Trp Ala
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Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
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Gly Tyr Lys Tyr Leu Gly Leu Phe Asn Gly Leu Asp Lys Gly Glu Pro
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Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
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Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
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Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
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Gln Pro Pro Ala Ala Pro Pro Pro Val Gly Ser Asn Thr Met Ala Ser
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Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
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Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
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Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
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Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
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Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
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Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
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Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
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Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
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Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
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Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
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Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
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Val Leu Val Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
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Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
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Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
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Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
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Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
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Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
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Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
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Gln Pro Pro Ala Ala Pro Pro Pro Val Gly Ser Asn Thr Met Ala Ser
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Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
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Gly Asn Ala Ser Gly Asn Trp His Arg Asp Ser Thr Trp Leu Gly Asp
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Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
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Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
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Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
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Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
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Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
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Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
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Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
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Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
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Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
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Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
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Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
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Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
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Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
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Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
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Val Leu Ile Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
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Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
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Asn Pro Thr Thr Glu Thr Val Asn Ser Gln Gly Ile Leu Pro Gly Met
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Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
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Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
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Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
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Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
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Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
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Glu Leu Gln Lys Glu Asn Gly Lys Arg Trp Asn Pro Glu Ile Gln Tyr
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Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Arg Arg
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Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
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Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
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Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
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Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
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Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
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Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Cys Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Ala Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
545 550 555 560
Val Leu Ile Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asp Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 6
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba30）
<400> 6
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Gly Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Leu
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Ala Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
545 550 555 560
Val Leu Ile Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asp Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 7
<211> 742
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(742)
<223> VP1序列（来自狒狒；标示为Bba31）
<400> 7
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Gln Arg Leu Ala Thr Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Val Lys Thr Ala Pro Gly Lys Lys His
130 135 140
Pro Leu Glu Lys Thr Pro Asn Arg Pro Thr Asn Pro Asp Ser Gly Lys
145 150 155 160
Ala Pro Ala Lys Lys Lys Gln Lys Asp Gly Glu Thr Ala Asp Ser Ala
165 170 175
Arg Arg Thr Leu Asp Phe Glu Asp Ser Gly Ala Gly Asp Gly Pro Pro
180 185 190
Glu Gly Ser Ser Ser Gly Glu Met Ser His Asp Ala Glu Met Arg Ala
195 200 205
Ala Pro Gly Gly Asn Ala Val Glu Ala Gly Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asp Trp His Cys Gly Ser Thr Trp Ser Glu Gly
225 230 235 240
Arg Val Thr Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Leu Tyr Leu Arg Ile Gly Thr Thr Ala Asn Ser Asn Thr Tyr
260 265 270
Asn Gly Phe Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Leu Arg Pro Lys Ser Met Arg Val Lys Ile Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Thr Ser Asn Gly Glu Thr Thr Val Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Ile Phe Ala Asp Ser Thr Tyr Glu Leu Pro Tyr
340 345 350
Val Met Asp Ala Gly Gln Glu Gly Ser Leu Pro Pro Phe Pro Asn Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Cys Gly Val Val Thr Gly Glu
370 375 380
Asn Gln Asn Gln Thr Asp Arg Asn Ala Phe Tyr Cys Leu Glu Cys Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Ile Ser Tyr Gln
405 410 415
Phe Glu Lys Val Pro Phe His Ser Met Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Met Met Asn Pro Leu Leu Asp Gln Tyr Leu Trp His Leu Gln
435 440 445
Ser Thr Thr Thr Gly Asn Ser Leu Asn Gln Gly Thr Ala Thr Thr Thr
450 455 460
Tyr Gly Lys Ile Thr Thr Gly Asp Phe Ala Tyr Tyr Arg Lys Asn Trp
465 470 475 480
Leu Pro Gly Ala Cys Ile Lys Gln Gln Lys Phe Ser Lys Asn Ala Ser
485 490 495
Gln Asn Tyr Lys Ile Pro Ala Ser Gly Gly Asp Ala Leu Leu Lys Tyr
500 505 510
Asp Thr His Thr Thr Leu Asn Gly Arg Trp Ser Asn Met Ala Pro Gly
515 520 525
Pro Pro Met Ala Thr Ala Gly Ala Gly Asp Ser Asp Phe Ser Asn Ser
530 535 540
Gln Leu Ile Phe Ala Gly Pro Asn Gln Ser Gly Asn Thr Thr Thr Ser
545 550 555 560
Ser Asn Asn Leu Leu Phe Thr Ser Glu Glu Glu Ile Ala Ala Thr Asn
565 570 575
Pro Arg Asp Thr Asp Met Phe Gly Gln Ile Ala Asp Asn Asn Gln Asn
580 585 590
Ala Thr Thr Ala Pro His Ile Ala Asn Leu Asp Ala Met Gly Ile Val
595 600 605
Pro Gly Met Val Trp Gln Asn Arg Asp Ile Tyr Tyr Gln Gly Pro Ile
610 615 620
Trp Ala Lys Val Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu
625 630 635 640
Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile Phe Ile Lys
645 650 655
Asn Thr Pro Val Pro Ala Asn Pro Asn Thr Thr Phe Ser Ala Ala Arg
660 665 670
Ile Asn Ser Phe Leu Thr Gln Tyr Ser Thr Gly Gln Val Ala Val Gln
675 680 685
Ile Asp Trp Glu Ile Gln Lys Glu His Ser Lys Arg Trp Asn Pro Glu
690 695 700
Val Gln Phe Thr Ser Asn Tyr Gly Thr Gln Asn Ser Met Leu Trp Ala
705 710 715 720
Pro Asp Asn Ala Gly Asn Tyr His Glu Pro Arg Ala Ile Gly Ser Arg
725 730 735
Phe Leu Thr His His Leu
740
<210> 8
<211> 742
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(742)
<223> VP1序列（来自狒狒；标示为Bba32）
<400> 8
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Gln Arg Leu Ala Thr Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Arg Pro Thr Asn Pro Asp Ser Gly Lys
145 150 155 160
Ala Pro Ala Lys Lys Lys Gln Lys Asp Gly Glu Thr Ala Asp Ser Ala
165 170 175
Arg Arg Thr Leu Asp Phe Glu Asp Ser Gly Ala Gly Asp Gly Pro Pro
180 185 190
Glu Gly Ser Ser Ser Gly Glu Met Ser His Asp Ala Glu Met Arg Ala
195 200 205
Ala Pro Gly Gly Asn Ala Val Glu Ala Gly Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Gly Trp His Cys Asp Ser Thr Trp Ser Glu Gly
225 230 235 240
Arg Val Thr Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Leu Tyr Leu Arg Ile Gly Thr Thr Ala Asn Ser Asn Thr Tyr
260 265 270
Asn Gly Phe Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Leu Arg Pro Lys Ser Met Arg Val Lys Ile Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Thr Ser Asn Gly Glu Thr Thr Val Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Ile Phe Ala Asp Ser Thr Tyr Glu Leu Pro Tyr
340 345 350
Val Met Asp Ala Gly Gln Glu Gly Ser Leu Pro Pro Phe Pro Asn Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Cys Gly Val Val Thr Gly Glu
370 375 380
Asn Gln Asn Gln Thr Asp Arg Asn Ala Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Ile Ser Tyr Gln
405 410 415
Phe Glu Lys Val Pro Phe His Ser Met Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Met Met Asn Pro Leu Leu Asp Gln Tyr Leu Trp His Leu Gln
435 440 445
Ser Thr Thr Thr Gly Asn Ser Leu Asn Gln Gly Thr Ala Thr Thr Thr
450 455 460
Tyr Gly Lys Ile Thr Thr Gly Asp Phe Ala Tyr Tyr Arg Lys Asn Trp
465 470 475 480
Leu Pro Gly Ala Cys Ile Lys Gln Gln Lys Phe Ser Lys Asn Ala Ser
485 490 495
Gln Asn Tyr Lys Ile Pro Ala Ser Gly Gly Asp Ala Leu Leu Lys Tyr
500 505 510
Asp Thr His Thr Thr Leu Asn Gly Arg Trp Ser Ser Met Ala Pro Gly
515 520 525
Pro Pro Met Ala Thr Ala Gly Ala Gly Asp Ser Asp Phe Ser Asn Ser
530 535 540
Gln Leu Ile Phe Ala Gly Pro Asn Gln Ser Gly Asn Thr Thr Thr Ser
545 550 555 560
Ser Asn Asn Leu Leu Phe Thr Ser Glu Glu Glu Ile Ala Thr Thr Asn
565 570 575
Pro Arg Asp Thr Asp Met Phe Gly Gln Ile Ala Asp Asn Asn Gln Asn
580 585 590
Ala Thr Thr Ala Pro His Ile Ala Asn Leu Asp Ala Met Gly Ile Val
595 600 605
Pro Gly Met Val Trp Gln Asn Arg Asp Ile Tyr Tyr Gln Gly Pro Ile
610 615 620
Trp Ala Lys Val Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu
625 630 635 640
Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile Phe Ile Lys
645 650 655
Asn Thr Pro Val Pro Ala Asn Pro Asn Thr Thr Phe Ser Ala Ala Arg
660 665 670
Ile Asn Ser Phe Leu Thr Gln Tyr Ser Thr Gly Gln Val Ala Val Gln
675 680 685
Ile Asp Trp Glu Ile Gln Lys Glu His Ser Lys Arg Trp Asn Pro Glu
690 695 700
Val Gln Phe Thr Ser Asn Tyr Gly Thr Gln Asn Ser Met Leu Trp Ala
705 710 715 720
Pro Gly Asn Ala Gly Asn His His Glu Pro Arg Ala Ile Gly Ser Arg
725 730 735
Phe Leu Thr His His Leu
740
<210> 9
<211> 742
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(742)
<223> VP1序列（来自狒狒；标示为Bba33）
<400> 9
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Gln Arg Leu Ala Thr Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Val Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Arg Pro Thr Asn Pro Asp Ser Gly Arg
145 150 155 160
Ala Pro Ala Lys Lys Lys Gln Lys Asp Gly Glu Thr Ala Asp Ser Ala
165 170 175
Arg Arg Thr Leu Asp Phe Glu Asp Ser Gly Ala Gly Asp Gly Pro Pro
180 185 190
Glu Gly Ser Ser Ser Gly Glu Met Ser His Asp Ala Glu Met Arg Ala
195 200 205
Ala Pro Gly Gly Asn Ala Val Glu Ala Gly Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asp Trp His Cys Asp Ser Thr Trp Ser Glu Gly
225 230 235 240
Arg Val Thr Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Leu Tyr Leu Arg Ile Gly Thr Thr Ala Asn Ser Asn Thr Tyr
260 265 270
Asn Gly Phe Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His
275 280 285
Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp
290 295 300
Gly Leu Arg Pro Lys Ser Met Arg Val Lys Ile Phe Asn Ile Gln Val
305 310 315 320
Lys Glu Val Thr Thr Ser Asn Gly Glu Thr Thr Val Ala Asn Asn Leu
325 330 335
Thr Ser Thr Val Gln Ile Phe Ala Asp Ser Thr Tyr Glu Leu Pro Tyr
340 345 350
Val Met Asp Ala Gly Gln Glu Gly Ser Leu Pro Pro Phe Pro Asn Asp
355 360 365
Val Phe Met Val Pro Gln Tyr Gly Tyr Cys Gly Val Val Thr Gly Glu
370 375 380
Asn Gln Asn Gln Thr Asp Arg Asn Ala Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Ile Ser Tyr Gln
405 410 415
Phe Glu Lys Val Pro Phe His Ser Met Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Met Met Asn Pro Leu Leu Asp Gln Tyr Leu Trp His Leu Gln
435 440 445
Ser Thr Thr Thr Gly Asn Ser Leu Asn Gln Gly Thr Ala Thr Thr Thr
450 455 460
Tyr Gly Lys Ile Thr Thr Gly Asp Phe Ala Tyr Tyr Arg Lys Asn Trp
465 470 475 480
Leu Pro Gly Ala Cys Ile Lys Gln Gln Lys Phe Ser Lys Asn Ala Ser
485 490 495
Gln Asn Tyr Lys Ile Pro Ala Ser Gly Gly Asp Ala Leu Leu Lys Tyr
500 505 510
Asp Thr His Thr Thr Leu Asn Gly Arg Trp Ser Asn Met Ala Pro Gly
515 520 525
Pro Pro Met Ala Thr Ala Gly Ala Gly Asp Ser Asp Phe Ser Asn Ser
530 535 540
Gln Leu Ile Phe Ala Gly Pro Asn Gln Ser Gly Asn Thr Thr Thr Ser
545 550 555 560
Ser Asn Asn Leu Leu Leu Thr Ser Glu Glu Glu Ile Ala Thr Thr Asn
565 570 575
Pro Arg Asp Thr Asp Met Phe Gly Gln Ile Ala Asp Asn Asn Gln Asn
580 585 590
Ala Thr Thr Ala Pro His Ile Ala Asn Leu Asp Ala Met Gly Ile Val
595 600 605
Pro Gly Met Val Trp Gln Asn Arg Asp Ile Tyr Tyr Gln Gly Pro Ile
610 615 620
Trp Ala Lys Val Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu
625 630 635 640
Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln Ile Phe Ile Lys
645 650 655
Asn Thr Pro Val Pro Ala Asn Pro Asn Thr Thr Phe Ser Ala Ala Arg
660 665 670
Ile Asn Ser Phe Leu Thr Gln Tyr Ser Thr Gly Gln Val Ala Val Gln
675 680 685
Ile Asp Trp Glu Ile Gln Lys Glu His Ser Lys Arg Trp Asn Pro Glu
690 695 700
Val Gln Phe Thr Ser Asn Tyr Gly Thr Gln Asn Ser Met Leu Trp Ala
705 710 715 720
Pro Gly Asn Ala Gly Asn Tyr His Glu Pro Arg Ala Ile Gly Ser Arg
725 730 735
Phe Leu Thr His His Leu
740
<210> 10
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba34）
<400> 10
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Ile Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Val Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Lys Thr Asn Ala Thr Asn Lys Thr Thr Leu Glu Asn
545 550 555 560
Val Leu Val Thr Asp Glu Glu Glu Val Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ser Asn Thr Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Ala Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 11
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba35）
<400> 11
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Val Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Ala Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Arg Val
530 535 540
Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
545 550 555 560
Val Leu Ile Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
580 585 590
Asp Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Val Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Thr Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Gly Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 12
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba36）
<400> 12
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Tyr Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Pro Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Pro Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Ala Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
545 550 555 560
Val Leu Ile Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 13
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba37）
<400> 13
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Glu Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Thr Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Ile Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Ala Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
545 550 555 560
Val Leu Ile Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 14
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba38）
<400> 14
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Gly Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Cys Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Ala Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
545 550 555 560
Val Leu Ile Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Thr Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 15
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba41）
<400> 15
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Ser Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Ala Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Gln Thr Asn Ala Thr Asn Lys Thr Thr Leu Asp Asn
545 550 555 560
Val Leu Ile Thr Ser Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ala Ser Asn Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 16
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba42）
<400> 16
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Gly Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Val Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Lys Thr Asn Ala Thr Asn Lys Thr Thr Leu Glu Asn
545 550 555 560
Val Leu Val Thr Asp Glu Glu Glu Val Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ser Asn Thr Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Leu Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 17
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba43）
<400> 17
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asn Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Val Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Lys Thr Asn Ala Thr Asn Lys Thr Thr Leu Glu Asn
545 550 555 560
Val Leu Val Thr Asp Glu Glu Glu Val Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ser Asn Thr Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Val Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 18
<211> 739
<212> PRT
<213> 埃及狒狒
<220>
<221> misc_feature
<222> (1)..(739)
<223> VP1序列（来自狒狒；标示为Bba44）
<400> 18
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln His Gln Asp Asn Ser Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Lys Gln Leu Glu Gln Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Leu Glu Lys Thr Pro Asn Gln Pro Thr Asp Thr Gln Ala Ala Gly
145 150 155 160
Gln Thr Pro Ala Lys Lys Arg Pro Gln Gly Glu Gln Ser Gly Asp Ser
165 170 175
Ala Arg Arg Gln Leu Asp Phe Asp Pro Gln Pro Ala Ala Pro Ile Gly
180 185 190
Gln Pro Pro Ala Ala Pro Ser Pro Val Gly Ser Asn Thr Met Ala Ser
195 200 205
Gly Gly Gly Gly Pro Met Ala Asp Asp Asn Gln Gly Ala Asp Gly Val
210 215 220
Gly Asn Ala Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp
225 230 235 240
Arg Val Ile Thr Thr Ser Thr Arg Thr Trp Val Leu Pro Thr Tyr Asn
245 250 255
Asn His Ile Tyr Lys Gln Ile Ser Ser Glu Ser Gly Ala Thr Asn Asp
260 265 270
Asn His Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn
275 280 285
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn
290 295 300
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Ile Phe Asn
305 310 315 320
Ile Gln Val Lys Glu Val Thr Gln Ile Glu Gly Gly Ser Thr Ile Ala
325 330 335
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Ala Asp Ser Glu Tyr Gln
340 345 350
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe
355 360 365
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn
370 375 380
Asn Gly Ser Gln Ser Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr
385 390 395 400
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr
405 410 415
Thr Phe Glu Ser Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser
420 425 430
Leu Asp Arg Ile Met Asn Pro Leu Val Asp Gln Tyr Leu Tyr Tyr Leu
435 440 445
Ala Arg Thr Gln Thr Gly Thr Gly Ser Thr Thr Ser Asn Thr Arg Gln
450 455 460
Leu Gln Phe Tyr Gln Ala Gly Pro Ser Asn Met Ala Asp Gln Ser Arg
465 470 475 480
Asn Trp Leu Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Lys Thr
485 490 495
Leu Asp Gln Asp Ser Asn Thr Asn Phe Ala Trp Thr Ala Ala Ser Lys
500 505 510
Tyr Asn Leu Asn Gly Arg Lys Ser Leu Val Asn Pro Gly Ile Ala Met
515 520 525
Ala Thr His Lys Asp Asp Glu Glu Arg Phe Phe Pro Gln His Gly Val
530 535 540
Leu Val Phe Gly Lys Thr Asn Ala Thr Asn Lys Thr Thr Leu Glu Asn
545 550 555 560
Val Leu Val Thr Asp Glu Glu Glu Val Lys Ala Thr Asn Pro Val Ala
565 570 575
Thr Glu Glu Tyr Gly Thr Val Ser Ser Asn Leu Gln Ser Asn Thr Thr
580 585 590
Asn Pro Thr Thr Glu Thr Val Asn Asn Gln Gly Ile Leu Pro Gly Met
595 600 605
Ala Trp Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys
610 615 620
Ile Pro His Thr Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly
625 630 635 640
Phe Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro
645 650 655
Val Pro Ala Asn Pro Pro Glu Thr Phe Thr Thr Ser Lys Phe Ala Ser
660 665 670
Tyr Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp
675 680 685
Glu Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr
690 695 700
Thr Ser Asn Tyr Ala Lys Ser Asn Asn Val Glu Phe Ser Val Asp Ala
705 710 715 720
Ala Gly Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730 735
Arg Asn Leu
<210> 19
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴；标示为Bce14）
<400> 19
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Ala Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Val Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Arg Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 20
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴；标示为Bce15)
<400> 20
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Pro Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Ala Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 21
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴（标示为Bce16））
<400> 21
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Pro Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 22
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴（标示为Bce17））
<400> 22
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ala Gln Pro Ala Ile Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Ile Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Ser Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 23
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴（标示为Bce18））
<400> 23
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 24
<211> 733
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(733)
<223> VP1序列（来自食蟹猕猴（标示为Bce20））
<400> 24
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly
145 150 155 160
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Arg Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn
260 265 270
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Ala Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Glu
405 410 415
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser
450 455 460
Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn
485 490 495
Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys
515 520 525
Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile
545 550 555 560
Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser
565 570 575
Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln
580 585 590
Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln
595 600 605
Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr
725 730
<210> 25
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bce35)
<400> 25
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Leu Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Thr Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Ser Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Lys Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Leu Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Thr Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Gly Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 26
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bce36））
<400> 26
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Thr Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Lys Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Leu Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Thr Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Asp Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 27
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴（标示为Bce39））
<400> 27
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Pro Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Phe Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Lys Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Ala Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Glu Val Ala Ile Asn Asp Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Met His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Ser Asp Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 28
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴（标示为Bce40））
<400> 28
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Gly Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Pro Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Ala Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Lys Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Arg Phe Ser Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Ala Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Glu Val Ala Ile Asn Asp Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Ser Asp Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 29
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴（标示为Bce41））
<400> 29
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Gly Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Ile Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Lys Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Ala Gly Asn Asp Gly Met Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Glu Val Ala Ile Asn Asp Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Ser Asp Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 30
<211> 736
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自食蟹猕猴（标示为Bce42））
<400> 30
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Gly Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly His Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Lys Asp Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asn Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Ala Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Glu Val Ala Val Asn Asp Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Leu Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Ser Asp Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 31
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bce43））
<400> 31
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Pro Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Pro Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Pro Tyr Tyr Leu Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Thr Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Lys Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Leu Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Ala Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Asp Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 32
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bce44））
<400> 32
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Met Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Ser Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Arg Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Pro Tyr Tyr Leu Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Thr Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Lys Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Pro Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Thr Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Asn Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Asp Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 33
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bce45））
<400> 33
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Ser Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asp His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Thr Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Lys Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Leu Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Thr Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Arg Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Asp Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 34
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bce46））
<400> 34
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Met Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Pro Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Thr Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Lys Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Leu Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Thr Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asp Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Asp Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 35
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bey20））
<400> 35
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Gly Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Val Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Ser Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Lys Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Leu Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Thr Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Asp Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 36
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bey22））
<400> 36
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Arg Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Ile Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Glu Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asp Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Thr Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Lys Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Leu Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Leu Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Thr Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Asp Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 37
<211> 730
<212> PRT
<213> 食蟹猴
<220>
<221> misc_feature
<222> (1)..(730)
<223> VP1序列（来自食蟹猕猴（标示为Bey23））
<400> 37
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Arg Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Asp Ser Pro Asp Ser Thr Ser Gly Ile Gly Lys Lys Gly Gln
145 150 155 160
Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr Gly Asp Ala Glu
165 170 175
Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro Ala Ala Pro Ser
180 185 190
Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly Gly Ala Pro Met Ala
195 200 205
Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asn Trp
210 215 220
His Cys Asp Ser Thr Trp Leu Gly Asn Arg Val Ile Thr Thr Ser Thr
225 230 235 240
Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu Tyr Lys Gln Ile
245 250 255
Ser Ser Ser Ser Ser Gly Ala Thr Asn Asp Asn His Tyr Phe Gly Tyr
260 265 270
Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Cys His Phe
275 280 285
Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp Gly Phe Arg
290 295 300
Pro Lys Arg Leu Arg Phe Lys Leu Phe Asn Ile Gln Val Lys Glu Val
305 310 315 320
Thr Thr Asn Asp Gly Val Thr Thr Ile Ala Asn Asn Leu Thr Ser Thr
325 330 335
Val Gln Val Phe Ser Asp Ser Glu Tyr Gln Leu Pro Tyr Val Leu Gly
340 345 350
Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp Val Phe Met
355 360 365
Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser Gln Ser Val
370 375 380
Gly Arg Ser Ser Phe Tyr Arg Leu Glu Tyr Phe Pro Ser Gln Met Leu
385 390 395 400
Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr Glu Phe Glu Asp Val Pro
405 410 415
Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg Leu Met Asn
420 425 430
Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ala Arg Thr Gln Ser Ser
435 440 445
Thr Gly Thr Ser Thr Arg Glu Leu Gln Phe His Gln Ala Gly Pro Ala
450 455 460
Thr Met Ala Glu Gln Ser Lys Asn Trp Leu Pro Gly Pro Cys Phe Arg
465 470 475 480
Gln Gln Arg Ile Ser Lys Thr Thr Asp Asn Asn Asn Asn Ser Asn Phe
485 490 495
Ala Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly Arg Asn Ser Leu
500 505 510
Thr Asn Pro Gly Val Pro Met Ala Thr His Arg Asp Asp Glu Ser Val
515 520 525
Phe Phe Pro Ile Asn Gly Val Leu Val Phe Gly Lys Thr Gly Ala Ser
530 535 540
Asn Lys Thr Thr Leu Glu Asn Val Leu Met Thr Asp Glu Glu Glu Ile
545 550 555 560
Lys Ala Thr Asn Pro Val Ala Thr Glu Glu Tyr Gly Val Val Ser Ser
565 570 575
Asn Ile Gln Ser Gln Asn Ser Asn Pro Thr Thr Gln Thr Val Asn Asn
580 585 590
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu
595 600 605
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His
610 615 620
Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys His Pro Pro Pro Gln
625 630 635 640
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn Pro Pro Glu Thr Phe
645 650 655
Thr Pro Ala Lys Phe Ala Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln
660 665 670
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg
675 680 685
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Asp Lys Gln Thr Gly
690 695 700
Val Asp Phe Ala Val Asp Thr Gln Gly Val Tyr Ser Glu Pro Arg Pro
705 710 715 720
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730
<210> 38
<211> 736
<212> PRT
<213> 普通狨
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自狨猴（标示为Bma42））
<400> 38
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Leu Arg Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Pro Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Pro Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala Arg Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asp Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Ala Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Leu Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 39
<211> 736
<212> PRT
<213> 普通狨
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自狨猴（标示为Bma43））
<400> 39
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Asn Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Arg Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Ser
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Ala Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 40
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpol））
<400> 40
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asp Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 41
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo2））
<400> 41
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Ala Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Gly Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 42
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo3））
<400> 42
Met Ser Phe Val Asp His Pro Pro Asp Trp Phe Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Ala
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Gly
385 390 395 400
Glu Val Pro Leu His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln His Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Pro Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Thr Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 43
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo4））
<400> 43
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 44
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo6））
<400> 44
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Gly Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asp Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Gly Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Leu Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 45
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo8））
<400> 45
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Lys Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Gly Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Ala
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Leu Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 46
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo13））
<400> 46
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Ala Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 47
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo18））
<400> 47
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asp Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Arg Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 48
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo20））
<400> 48
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Gly Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Gly Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 49
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo23））
<400> 49
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Arg Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Val Asp Glu Val Ala Arg Glu Arg Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Leu Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 50
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo24））
<400> 50
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Cys Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 51
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo27））
<400> 51
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asp Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Val Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asn Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 52
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo28））
<400> 52
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Arg Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Gly Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Leu
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Ala Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Ser Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Arg Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 53
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo29））
<400> 53
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Gly Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Gly
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Val Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 54
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo33））
<400> 54
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala His Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 55
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo35））
<400> 55
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Arg Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Gly Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 56
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo36））
<400> 56
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Glu Pro Gly Pro Pro Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Gly Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Pro Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Leu Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Ser Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Val
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asn Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Phe Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 57
<211> 716
<212> PRT
<213> 野猪
<220>
<221> misc_feature
<222> (1)..(716)
<223> VP1序列（来自猪（标示为Bpo37））
<400> 57
Met Ser Phe Val Asp His Pro Pro Asp Trp Leu Glu Glu Ile Gly Glu
1 5 10 15
Gly Leu Lys Glu Phe Leu Gly Leu Lys Pro Gly Pro Leu Lys Pro Lys
20 25 30
Pro Asn Gln Gln Lys Gln Asp Asn Ala Arg Gly Leu Val Leu Pro Gly
35 40 45
Tyr Asn Tyr Leu Gly Pro Gly Asn Gly Leu Asp Arg Gly Glu Pro Val
50 55 60
Asn Arg Ala Asp Glu Val Ala Arg Glu His Asp Ile Ser Tyr Asn Glu
65 70 75 80
Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala Asp
85 90 95
Ala Glu Phe Gln Glu Lys Leu Ala Asp Asp Thr Ser Phe Gly Gly Asn
100 105 110
Leu Gly Lys Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro Phe
115 120 125
Gly Leu Val Glu Glu Pro Ala Lys Thr Ala Ala Lys Gly Glu Arg Ile
130 135 140
Asp Asp His Tyr Pro Lys Lys Lys Lys Ala Arg Val Glu Glu Thr Glu
145 150 155 160
Ala Gly Thr Ser Gly Gly Gln Gln Leu Gln Ile Pro Ala Gln Pro Ala
165 170 175
Ser Ser Leu Gly Ala Asp Thr Met Ser Ala Gly Gly Gly Ser Pro Leu
180 185 190
Gly Asp Asn Asn Gln Gly Ala Asp Gly Val Gly Asn Ala Ser Gly Asp
195 200 205
Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile Thr Lys Ser
210 215 220
Thr Arg Thr Trp Val Leu Pro Ser Tyr Asn Asn His Leu Tyr Lys Glu
225 230 235 240
Ile His Ser Gly Ser Val Asp Gly Ser Asn Ala Asn Ala Tyr Phe Gly
245 250 255
Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His Ser His
260 265 270
Trp Ser Pro Arg Asp Trp Gln Arg Leu Val Asn Asn Tyr Trp Gly Phe
275 280 285
Arg Pro Arg Ser Leu Lys Val Lys Ile Phe Asn Ile Gln Val Lys Glu
290 295 300
Val Thr Val Gln Asp Ala Thr Thr Thr Ile Ala Asn Asn Leu Thr Ser
305 310 315 320
Thr Val Gln Val Phe Thr Asp Asp Asp Tyr Gln Leu Pro Tyr Val Ile
325 330 335
Gly Asn Gly Thr Glu Gly Cys Leu Pro Ala Phe Pro Pro Gln Val Phe
340 345 350
Thr Leu Pro Gln Tyr Gly Tyr Ala Thr Leu Asn Arg Asn Asn Thr Asp
355 360 365
Asp Pro Thr Glu Arg Ser Ser Phe Phe Cys Leu Glu Tyr Phe Pro Ser
370 375 380
Lys Met Leu Arg Thr Gly Asn Ser Phe Glu Phe Thr Tyr Ser Phe Glu
385 390 395 400
Glu Val Pro Phe His Cys Ser Phe Ala Pro Ser Gln Asn Leu Phe Lys
405 410 415
Leu Ala Asn Pro Leu Val Asp Gln Tyr Leu Tyr Arg Phe Val Ser Thr
420 425 430
Asp Thr Pro Gly Asn Ile Gln Phe Gln Lys Asn Leu Lys Ala Arg Tyr
435 440 445
Ala Asn Thr Tyr Lys Asn Trp Phe Pro Gly Pro Met Cys Arg Thr Gln
450 455 460
Gly Trp Tyr Thr Gly Ser Gly Thr Tyr Asn Arg Ser Gly Val Thr Asn
465 470 475 480
Phe Ala Thr Ser Asn Arg Met Asp Leu Glu Gly Ala Ser Tyr Gln Ala
485 490 495
Asn Pro Gln Pro Asn Gly Met Thr Asn Thr Leu Gln Asp Ser Asn Lys
500 505 510
Tyr Ala Leu Glu Asn Thr Met Ile Phe Asn Ser Gln Asn Ala Glu Pro
515 520 525
Gly Thr Thr Ser Leu Tyr Gln Glu Asn Asn Leu Leu Ile Thr Ser Glu
530 535 540
Ser Glu Thr Gln Pro Val Asn Arg Val Ala Tyr Asp Thr Gly Gly Gln
545 550 555 560
Met Ala Thr Asn Ala Gln Ser Thr Asn Leu Ala Pro Thr Val Gly Thr
565 570 575
Tyr Asn His Gln Glu Met Leu Pro Gly Ser Val Trp Met Asp Arg Asp
580 585 590
Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro Glu Thr Gly Ala
595 600 605
His Phe His Pro Ser Pro Ala Met Gly Gly Phe Gly Leu Lys His Pro
610 615 620
Pro Pro Met Met Leu Ile Lys Asn Thr Pro Val Pro Ser Asn Val Thr
625 630 635 640
Ala Phe Ser Glu Ile Pro Val Lys Ser Leu Ile Thr Gln Tyr Ser Thr
645 650 655
Gly Gln Val Thr Val Glu Met Glu Trp Glu Leu Lys Lys Glu Asn Ser
660 665 670
Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Asn Asn Tyr Asn Asn Pro
675 680 685
Glu Phe Val Asp Phe Ala Pro Asp Thr Ser Gly Glu Tyr Arg Thr Thr
690 695 700
Arg Ala Ile Gly Thr Arg Tyr Leu Thr Arg Pro Leu
705 710 715
<210> 58
<211> 736
<212> PRT
<213> 猕猴(Macaca mulatta)
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自恒河猴（标示为Brh26））
<400> 58
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Ala Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Ile Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Gly
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Asp Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 59
<211> 736
<212> PRT
<213> 猕猴(Macaca mulatta)
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自恒河猴（标示为Brh27））
<400> 59
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Ile Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Pro
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Arg Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Ser Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Asp Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys Leu Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 60
<211> 736
<212> PRT
<213> 猕猴(Macaca mulatta)
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自恒河猴（标示为Brh28））
<400> 60
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Ile Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Pro Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Asp Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 61
<211> 736
<212> PRT
<213> 猕猴(Macaca mulatta)
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自恒河猴（标示为Brh29））
<400> 61
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Ile Phe Gln Ala Lys Arg Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Ser Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Cys Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Gly Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Asp Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Pro Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 62
<211> 736
<212> PRT
<213> 猕猴(Macaca mulatta)
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自恒河猴（标示为Brh30））
<400> 62
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Phe Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Ile Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Arg Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Pro Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Ala Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Asp Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Gln Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 63
<211> 736
<212> PRT
<213> 猕猴(Macaca mulatta)
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自恒河猴（标示为Brh31））
<400> 63
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Ile Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Ser Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala Tyr Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Ser Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Asp Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 64
<211> 736
<212> PRT
<213> 猕猴(Macaca mulatta)
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自恒河猴（标示为Brh32））
<400> 64
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Ser Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Val Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala Tyr Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Asp Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 65
<211> 736
<212> PRT
<213> 猕猴(Macaca mulatta)
<220>
<221> misc_feature
<222> (1)..(736)
<223> VP1序列（来自恒河猴（标示为Brh33））
<400> 65
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Ser Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Val Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Thr Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Gly Leu Gly Pro Asn Thr Met Ala Ser Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Thr Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala Tyr Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Leu Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Val
435 440 445
Arg Thr Gln Thr Thr Gly Thr Gly Gly Thr Gln Thr Leu Ala Phe Ser
450 455 460
Gln Ala Gly Pro Ser Ser Met Ala Asn Gln Ala Arg Asn Trp Val Pro
465 470 475 480
Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Thr Asn Gln Asn
485 490 495
Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Ala Lys Phe Lys Leu Asn
500 505 510
Gly Arg Asp Ser Leu Met Asn Pro Gly Val Ala Met Ala Ser His Lys
515 520 525
Asp Asp Asp Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Ile Phe Gly
530 535 540
Lys Gln Gly Thr Gly Asn Asp Gly Val Asp Tyr Ser Gln Val Leu Ile
545 550 555 560
Thr Asp Glu Glu Glu Ile Lys Ala Thr Asn Pro Val Ala Thr Glu Glu
565 570 575
Tyr Gly Ala Val Ala Ile Asn Asn Gln Ala Ala Asn Thr Gln Ala Gln
580 585 590
Thr Gly Leu Val His Asn Gln Gly Val Ile Pro Gly Met Val Trp Gln
595 600 605
Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His
610 615 620
Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu
625 630 635 640
Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala
645 650 655
Asp Pro Pro Leu Thr Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile Thr
660 665 670
Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln
675 680 685
Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn
690 695 700
Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly Val
705 710 715 720
Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu
725 730 735
<210> 66
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm17））
<400> 66
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Arg Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Ser Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Glu Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asp Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu His Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 67
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm18））
<400> 67
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Pro Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Pro Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 68
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm.20））
<400> 68
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Pro Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Phe Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Arg Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 69
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm21））
<400> 69
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Glu Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Ala Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Thr Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Ser
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 70
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm24））
<400> 70
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Pro Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Ser His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 71
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm25））
<400> 71
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Leu Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Val Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 72
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm27））
<400> 72
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Arg Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala Arg Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asp Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 73
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm32））
<400> 73
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asp Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 74
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm33））
<400> 74
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Ala Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Ser
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 75
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm34））
<400> 75
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
20 25 30
Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro
50 55 60
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
85 90 95
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
115 120 125
Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
130 135 140
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
165 170 175
Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
180 185 190
Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
210 215 220
Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
225 230 235 240
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Asn
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
275 280 285
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
290 295 300
Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
325 330 335
Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu
340 345 350
Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
385 390 395 400
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Thr
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
435 440 445
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
450 455 460
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
465 470 475 480
Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Tyr Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
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Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
<210> 76
<211> 737
<212> PRT
<213> 台湾猕猴(Macaca cyclopis)
<220>
<221> misc_feature
<222> (1)..(737)
<223> VP1序列（来自台湾猕猴(formosan macaque)（标示为Bfm35））
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Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser
1 5 10 15
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Lys Pro
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Lys Ala Asn Gln Gln Lys Gln Asp Asp Gly Arg Gly Leu Val Leu Pro
35 40 45
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Gly Pro
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Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp
65 70 75 80
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala
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Asp Ala Glu Phe Arg Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly
100 105 110
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro
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Leu Gly Leu Val Glu Glu Gly Ala Lys Thr Ala Pro Gly Lys Lys Arg
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Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ser Gly Ile Gly
145 150 155 160
Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr
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Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Leu Gly Glu Pro Pro
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Ala Ala Pro Ser Ser Leu Gly Leu Asn Thr Met Ala Ala Gly Gly Gly
195 200 205
Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser
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Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val Ile
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Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu
245 250 255
Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp Ser
260 265 270
Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg
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Ser His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn
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Asn Trp Gly Phe Arg Pro Lys Lys Leu Ser Phe Lys Leu Phe Asn Ile
305 310 315 320
Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala Asn
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Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro
355 360 365
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn
370 375 380
Gly Gly Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe
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Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Ser Phe Ser Tyr Ile
405 410 415
Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu
420 425 430
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser
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Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe
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Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu
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Pro Gly Pro Met Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln
485 490 495
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Gly Thr Lys Tyr His Leu
500 505 510
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr Asn
515 520 525
Lys Asp Asp Glu Asp Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe
530 535 540
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met
545 550 555 560
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu
565 570 575
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Thr Ala Pro
580 585 590
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp
595 600 605
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro
610 615 620
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly
625 630 635 640
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro
645 650 655
Ala Asp Pro Pro Thr Ala Phe Asn Gln Ala Lys Leu Asn Ser Phe Ile
660 665 670
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu
675 680 685
Leu Lys Glu Ser Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser
690 695 700
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly
705 710 715 720
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn
725 730 735
Leu
Claims (40)
1.一种用于制备重组腺相关病毒(rAAV)的方法，所述方法包括以下步骤：
在具有有效量的过渡金属的培养基中培养能够产生rAAV衣壳的宿主细胞；
其中有效量的所述过渡金属增加VP1、VP2或VP3蛋白到所述rAAV衣壳中的掺入。
2.一种用于制备重组腺相关病毒(rAAV)的方法，所述方法包括以下步骤：
在具有纳摩尔浓度的过渡金属的培养基中培养宿主细胞，所述宿主细胞能够产生rAAV衣壳。
3.一种用于制备重组腺相关病毒(rAAV)的方法，所述方法包括以下步骤：
在具有微摩尔浓度的过渡金属的培养基中培养宿主细胞，所述宿主细胞能够产生rAAV衣壳。
4.一种用于制备重组腺相关病毒(rAAV)的方法，所述方法包括以下步骤：
在具有有效量的过渡金属的培养基中培养能够产生rAAV衣壳的宿主细胞；
其中有效量的所述过渡金属增加VP1和VP3蛋白到所述rAAV衣壳中的掺入；
其中所述rAAV衣壳具有大于在相同条件下但缺乏所述有效量的所述过渡金属的情况下产生的rAAV衣壳的VP1和VP3蛋白浓度的VP1和VP3蛋白浓度。
5.一种用于制备重组腺相关病毒(rAAV)的方法，所述方法包括以下步骤：
在具有有效量的过渡金属的培养基中培养能够产生rAAV衣壳的宿主细胞；
其中有效量的所述过渡金属增加VP1蛋白到所述rAAV衣壳中的掺入。
6.一种用于制备重组腺相关病毒(rAAV)的方法，所述方法包括以下步骤：
在具有有效量的半胱氨酸蛋白酶抑制剂的培养基中培养宿主细胞，所述宿主细胞能够产生rAAV衣壳；
其中所述有效量的半胱氨酸蛋白酶抑制剂增加VP1、VP2或VP3蛋白到所述rAAV衣壳中的掺入。
7.一种rAAV衣壳，其通过根据权利要求1至6中任一项所述的方法制备，其中所述rAAV衣壳具有大于在相同条件下但缺乏所述有效量的所述过渡金属的情况下产生的rAAV衣壳的VP1、VP2或VP3蛋白浓度的VP1、VP2或VP3蛋白浓度。
8.一种rAAV衣壳，其通过根据权利要求1至6中任一项所述的方法制备，其中所述rAAV衣壳表现出增强的感染性。
9.根据权利要求1至6中任一项所述的方法，其中所述宿主细胞是非哺乳动物宿主细胞。
10.根据权利要求1至6中任一项所述的方法，其中所述宿主细胞是昆虫细胞。
11.根据权利要求1至5中任一项所述的方法，其中所述过渡金属选自以下中的一者或多者：铜、钪、钛、钒、铬、锰、铁、钴、镍、铜、锌、钇、锆、铌、钼、锝、钌、铑、钯、银、镉、铪、钽、钨、铼、锇、铱、铂、金、汞、 以及/>
12.根据权利要求1至5中任一项所述的方法，其中所述过渡金属是过渡金属盐。
13.根据权利要求1至5中任一项所述的方法，其中所述过渡金属选自以下中的一者或多者：硫酸铜、硝酸铜、硒化铜、氢氧化铜、氧化铜、磷酸铜、硅酸铜、硼酸铜、碳酸铜、氯化铝、氯化镁、硒化锂、碳酸钠、氯化锂、磷酸氢钠、偏硅酸钠、氢氧化锶、磷酸三钠、氟化钾、硫酸镁、氯化钙、硫酸钠、硫酸铝、四硼酸钠、硫酸镁、溴化镁、硫酸铝铷、氢氧化钡、硫酸铝钾、硝酸镁、磷酸氢钠、硫酸镍、硫酸锌、硫酸铍、硝酸锂、氯化锶、硝酸锌、焦磷酸钠、溴化钙、硝酸铜、硝酸铝、四硼酸钠、氟化银、碘化钙、溴化锂、碘化锂、溴化锶、硝酸钙、碘化锶、溴化钠和硝酸锶、乳酸钠铝、乙酸钠、脱氢乙酸钠、丁氧基乙氧基乙酸钠、辛酸钠、柠檬酸钠、乳酸钠、二羟基甘氨酸钠、葡萄糖酸钠、谷氨酸钠、羟基甲烷磺酸钠以及草酸钠。
14.根据权利要求1、4和5中任一项所述的方法，其中所述有效量的范围为约1nM至约1mM。
15.根据权利要求1、4和5中任一项所述的方法，其中所述有效量的范围为约10nM至约100μM。
16.根据权利要求1、4和5中任一项所述的方法，其中所述有效量的范围为约20μM至约25μM。
17.根据权利要求2所述的方法，其中所述过渡金属的所述纳摩尔浓度的范围为约1nM至小于1μM。
18.根据权利要求2所述的方法，其中所述过渡金属的所述纳摩尔浓度的范围为约10nM至小于1μM。
19.根据权利要求3所述的方法，其中所述过渡金属的所述微摩尔浓度的范围为约1μM至小于1mM。
20.根据权利要求3所述的方法，其中所述过渡金属的所述微摩尔浓度的范围为约1μM至约100μM。
21.根据权利要求3所述的方法，其中所述过渡金属的所述微摩尔浓度的范围为约20μM至约25μM。
22.根据权利要求1、4、5和6中任一项所述的方法，其中所述有效量足以增加VP1蛋白到所述rAAV衣壳中的掺入。
23.根据权利要求1、4和6中任一项所述的方法，其中所述有效量足以增加VP3蛋白到所述rAAV衣壳中的掺入。
24.根据权利要求1或6所述的方法，其中所述有效量足以增加VP2蛋白到所述rAAV衣壳中的掺入。
25.根据权利要求2所述的方法，其中所述过渡金属的所述纳摩尔浓度足以增加VP1、VP2或VP3蛋白到所述rAAV衣壳中的掺入。
26.根据权利要求3所述的方法，其中所述过渡金属的所述微摩尔浓度足以增加VP1、VP2或VP3蛋白到所述rAAV衣壳中的掺入。
27.根据权利要求1至6中任一项所述的方法，其进一步包括通过所述宿主细胞来分离所述rAAV衣壳的步骤。
28.根据权利要求1至6中任一项所述的方法，其进一步包括能够产生rAAV衣壳的宿主细胞。
29.根据权利要求1、4、5和6中任一项所述的方法，其进一步包括能够产生一定浓度的rAAV衣壳的宿主细胞，其中所述有效量降低rAAV衣壳的所述浓度。
30.根据权利要求1、4、5和6中任一项所述的方法，其进一步包括能够产生一定浓度的rAAV衣壳的宿主细胞，其中rAAV衣壳的所述浓度小于在相同条件下但缺乏所述有效量的情况下产生的rAAV衣壳的浓度。
31.根据权利要求2所述的方法，其进一步包括能够产生一定浓度的rAAV衣壳的宿主细胞，其中所述过渡金属的所述纳摩尔浓度降低rAAV衣壳的所述浓度。
32.根据权利要求2所述的方法，其进一步包括能够产生一定浓度的rAAV衣壳的宿主细胞，其中rAAV衣壳的所述浓度小于在相同条件下但缺乏所述过渡金属的所述纳摩尔浓度的情况下产生的rAAV衣壳的浓度。
33.根据权利要求3所述的方法，其进一步包括能够产生一定浓度的rAAV衣壳的宿主细胞，其中所述过渡金属的所述微摩尔浓度降低rAAV衣壳的所述浓度。
34.根据权利要求3所述的方法，其进一步包括能够产生一定浓度的rAAV衣壳的宿主细胞，其中rAAV衣壳的所述浓度小于在相同条件下但缺乏所述过渡金属的所述微摩尔浓度的情况下产生的rAAV衣壳的浓度。
35.根据权利要求1至6中任一项所述的方法，其中所述培养步骤在25毫升或更大的体积中进行。
36.根据权利要求1至6中任一项所述的方法，其中所述培养步骤在100毫升或更大的体积中进行。
37.根据权利要求1至6中任一项所述的方法，其中所述培养步骤在1升或更大的体积中进行。
38.根据权利要求1至6中任一项所述的方法，其中所述培养步骤在10升或更大的体积中进行。
39.根据权利要求1至6中任一项所述的方法，其中所述培养步骤在100升或更大的体积中进行。
40.根据权利要求1至6中任一项所述的方法，其中所述培养步骤在500升或更大的体积中进行。
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| US202163144104P | 2021-02-01 | 2021-02-01 | |
| US63/144,104 | 2021-02-01 | ||
| PCT/US2022/014058 WO2022165004A1 (en) | 2021-02-01 | 2022-01-27 | Aav production systems for aav viral particles with improved infectivity |
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| EP (1) | EP4284441A1 (zh) |
| JP (1) | JP2024505251A (zh) |
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