CN117025380A - Stem cell exosome culture device and method - Google Patents
Stem cell exosome culture device and method Download PDFInfo
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- CN117025380A CN117025380A CN202310855932.3A CN202310855932A CN117025380A CN 117025380 A CN117025380 A CN 117025380A CN 202310855932 A CN202310855932 A CN 202310855932A CN 117025380 A CN117025380 A CN 117025380A
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 40
- 210000001808 exosome Anatomy 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 6
- 230000001681 protective effect Effects 0.000 claims abstract description 60
- 238000003825 pressing Methods 0.000 claims description 31
- 238000004804 winding Methods 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000003806 hair structure Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 1
- 230000010355 oscillation Effects 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 57
- 239000012895 dilution Substances 0.000 abstract description 7
- 238000010790 dilution Methods 0.000 abstract description 7
- 239000011435 rock Substances 0.000 abstract description 4
- 238000004113 cell culture Methods 0.000 abstract description 3
- 238000007865 diluting Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 5
- 239000010902 straw Substances 0.000 description 5
- 238000012136 culture method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000003113 dilution method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000018448 secretion by cell Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
The invention provides a stem cell exosome culture device and a method, which relate to the technical field of stem cell culture and comprise the following steps: an outer protective case; one end of the bottom of the sliding control mechanism is inserted into the outer protective box in a sliding way; the top of the suction pipe assembly is connected with the sliding control mechanism; the surface of the suction control mechanism is fixedly connected with the top of the sliding control mechanism; the top of the culture solution placement device is inserted into the outer protective box; the culture solution placing bottle is arranged in the culture solution placing device; the vibration mechanism is arranged inside the outer protective box; the dilution operation of the present invention is performed in an approximately closed environment; when the culture solution is sucked, the culture solution accommodating bottle can be automatically opened or closed for protection; the culture dish can be automatically controlled to shake; the problem of the operation panel be open state, can produce certain influence to diluting the operation, frequently open and turn round tightly and close the bottle lid, complex operation, dilution efficiency reduces, and the culture dish needs the manual work to waste time and energy to rock is solved.
Description
Technical Field
The invention relates to the technical field of stem cell culture, in particular to a stem cell exosome culture device and a stem cell exosome culture method.
Background
Exosomes are a cellular secretion of stem cells; it contains proteins of stem cells and certain functions of stem cells; the stem cell exosome has the ability of crossing blood brain barrier, be the main medium of transfer information between stem cell and other cells, stem cell need the staff to dilute to stem cell in the culture process, divide into many bottles of cells and just can continue to grow, later stage separates stem cell and exosome through separator, in the stem cell dilution process, the mode that needs the staff to absorb the culture solution through the straw as far as possible in aseptic environment, the operation panel during dilution is open state, can exist the bacterium in the air, produce certain influence to diluting, and during the dilution, in order to guarantee that the liquid in the blake bottle is not influenced by outside air, when absorbing the liquid in the blake bottle, need frequently open and turn round tight the closing bottle lid, complex operation, dilution efficiency reduces, and the liquid need artifical rocks the blake dish of taking time and energy when the culture solution drops into the blake dish and mix stem cell and culture solution.
Disclosure of Invention
In view of the above, the present invention provides a stem cell exosome culture apparatus and method, which has an outer protective case, a sliding control mechanism and a suction control mechanism provided inside the apparatus, and a worker can control the suction control mechanism inside the outer protective case to perform a dilution operation in an approximately closed environment through the sliding control mechanism; the height control assembly can conveniently insert culture solution placing bottles with different heights into the outer protective box; the culture solution placing device can automatically open or close the culture solution placing bottle for protection when the culture solution is sucked, so that the efficiency is high; vibration mechanism can the automatic control culture dish rock, because the inside push pedal of device is provided with two sets of for the culture solution is more even, does not need manual rock, labour saving and time saving.
The invention provides a stem cell exosome culture device, which specifically comprises: the device comprises an outer protective box, a sliding control mechanism, a suction pipe assembly, a suction control mechanism, a culture solution placement device, a culture solution placement bottle and a vibration mechanism;
the whole outer protective box is of a cuboid structure with a hollow inside, and an ultraviolet disinfection lamp is fixedly arranged in the outer protective box; one end of the bottom of the sliding control mechanism is inserted into the outer protective box in a sliding way; the top of the suction pipe assembly is connected with the sliding control mechanism; the surface of the suction control mechanism is fixedly connected with the top of the sliding control mechanism, and one end of the suction control mechanism is connected with the suction pipe assembly; the top of the culture solution placement device is inserted into the outer protective box; the culture solution placing bottle is arranged in the culture solution placing device; the vibration mechanism is arranged inside the outer protective box, and the middle part of the vibration mechanism is contacted with the sliding control mechanism.
Optionally, the sliding control mechanism includes: the device comprises a baffle, a control bracket, a supporting rod A, a connecting plate and a pressing frame; the whole baffle is of an H-shaped structure and is slidably arranged in the chute of the outer protective box; the surface of the control bracket is in sliding connection with the baffle; the surface of the supporting rod A is provided with a spring, and the bottom of the supporting rod A is fixedly connected with the outer protective box through a square plate; one end of the connecting plate is fixedly connected with the baffle, and the surface of the connecting plate is connected with the support rod A in a sliding manner; the whole L-shaped structure of pressure frame, pressure frame quantity sets up into two sets of, pressure frame top one end and control bracket fixed connection.
Optionally, the straw assembly comprises: the device comprises a top column, a suction pipe, a pull rod, a plug board and a pull rope; the whole top column is of a circular tube structure, the top of the top column is fixedly connected with the control bracket, and a spring is arranged in the top column; the top of the suction pipe is fixedly connected with the jacking column; the pull rod is slidably arranged in the top column and the suction pipe, and the top of the pull rod is fixedly connected with the spring; the plug plate is fixedly arranged at the bottom of the pull rod; the bottom of the pull rope is fixedly connected with the pull rod, and the top of the pull rope slides through the control bracket and is inserted into the suction control mechanism.
Optionally, the suction control mechanism includes: the outer support box, the support rod B, the sliding frame and the linkage shaft; the surface of the outer supporting box is fixedly connected with the control bracket; the surface of the support rod B is provided with a spring, and the support rod B is fixedly arranged in the outer support box; the rack plate is fixedly arranged on the surface of the sliding frame, the whole sliding frame is of a T-shaped structure, and one end of the sliding frame is connected with the support rod B in a sliding manner; the surface of the linkage shaft is fixedly provided with a gear, the linkage shaft is rotatably arranged in the outer supporting box, and the gear on the surface of the linkage shaft is meshed and connected with the rack plate on the surface of the sliding frame.
Optionally, the suction control mechanism further includes: the driving shaft, the winding wheel and the control board; the surface of the driving shaft is fixedly provided with a gear, the driving shaft is rotatably arranged in the outer supporting box, and the gear on the surface of the driving shaft is meshed with the linkage shaft; the winding wheel is fixedly arranged in the middle of the driving shaft, and the surface of the winding wheel is connected with the other end of the pull rope; one end of the control board is inserted into the outer supporting box in a sliding way and is fixedly connected with the sliding frame.
Optionally, the culture solution placement device includes: the device comprises a height control assembly, a support rod C, a sliding baffle, a tension spring and a push plate A; the top of the height control component is fixedly connected with the outer protective box; the support rod C is fixedly arranged in the outer protective box; the sliding baffle is connected with the two groups of support rods C in a sliding way; the tension spring is arranged on the surface of the support rod C, one end of the tension spring is fixedly connected with the inner wall of the outer protective box, and the other end of the tension spring is fixedly connected with the sliding baffle; the whole push plate A is of a trapezoid structure, the push plate A is fixedly arranged on the surface of the sliding baffle, and the pressing frame is contacted with the inclined plane of the push plate A.
Optionally, the height control assembly further comprises: the support box, the screw rod, the main control rod and the bearing plate; the whole supporting box is of a cuboid structure with a hollow inside, a sliding groove is formed in the surface of the supporting box, and the top of the supporting box is fixedly connected with the outer protective box; the screw rod is rotatably arranged in the supporting box; one end of the main control rod is inserted into the supporting box in a rotating way, and one end of the main control rod is connected with the screw rod in a rotating way through a bevel gear; the whole bearing plate is of a T-shaped structure with a groove formed in the surface, and one end of the bearing plate is inserted into the supporting box in a sliding mode and is in threaded connection with the screw rod.
Optionally, the oscillating mechanism includes: the culture dish comprises a rotating rod, a rotating support, a culture dish support plate and an auxiliary pressing plate; the rotating rod is rotatably arranged in the outer protective box; the whole rotating support frame is of a V-shaped structure, and the bottom of the rotating support frame is fixedly connected with the rotating rod; the bottom of the culture dish support plate is fixedly connected with the rotary support frame; the whole right triangle structure that is of supplementary clamp plate, supplementary clamp plate quantity set up into two sets of, supplementary clamp plate bottom and culture dish extension board fixed connection.
Optionally, the oscillating mechanism further includes: the elastic telescopic rod, the linkage plate and the push plate B; one end of the elastic telescopic rod is fixedly connected with the inner wall of the outer protective box, and the other end of the elastic telescopic rod is contacted with the rotary supporting frame; the surface of the linkage plate is fixedly connected with the elastic telescopic rod, and the bottom of the linkage plate is contacted with the auxiliary pressing plate; the push plate B is fixedly arranged on the surface of the linkage plate, and the bottom of the pressing frame is contacted with the linkage plate.
The invention also provides a stem cell exosome culture method, which is used for completing the culture of stem cell exosomes by the stem cell exosome culture device.
Advantageous effects
The invention is internally provided with the outer protective box, the sliding control mechanism and the suction control mechanism, the interior of the outer protective box is in an approximately closed state, and the interior of the outer protective box is also provided with the sterilizing lamp, so that the outer protective box is in a sterile state, and a worker can conveniently control the suction control mechanism in the outer protective box to perform dilution operation through the sliding control mechanism, thereby preventing the culture solution from being polluted.
The height control assembly is arranged in the invention, the bearing plate capable of sliding in an adjustable way is arranged in the height control assembly, and the bearing plate can conveniently insert culture solution placing bottles with different heights into the outer protective box, so that the suction control mechanism can conveniently suck the culture solution.
The sliding control mechanism and the culture solution placement device are arranged in the culture solution placement bottle, when the sliding control mechanism controls the suction pipe to be pressed down, the sliding baffle plate in the sliding control mechanism is stressed to automatically slide to one side, the bottle mouth of the culture solution placement bottle is opened, the culture solution is conveniently sucked, after the suction is finished, the sliding baffle plate is controlled by the tension spring at one end to automatically return to the original position, the culture solution placement bottle is plugged and protected again, and the culture solution placement bottle does not need to be opened and closed by workers with time and effort.
The vibrating mechanism is arranged in the culture dish, when the suction pipe slides downwards and drops the culture solution to the culture dish, the support plate of the culture dish is stressed to automatically deflect and shake, and as the two groups of push plates in the device are arranged and are arranged in a crossed manner, the culture solution in the culture dish is more uniform, the culture solution does not need to shake manually, and the time and the labor are saved.
Drawings
In order to more clearly illustrate the technical solution of the embodiments of the present invention, the drawings of the embodiments will be briefly described below.
The drawings described below are only for illustration of some embodiments of the invention and are not intended to limit the invention.
In the drawings:
FIG. 1 shows a schematic structural diagram of a body shaft side view of the present invention;
FIG. 2 shows a schematic structural view of the present invention in cross-section of the body;
FIG. 3 shows a schematic cross-sectional structural view of the slide control mechanism of the present invention;
FIG. 4 shows a schematic cross-sectional structural view of the straw assembly of the present invention;
FIG. 5 shows a schematic axial side view of the suction control mechanism of the present invention;
FIG. 6 is a schematic diagram showing a side view of the culture solution placement device of the present invention;
FIG. 7 shows a schematic cross-sectional structural view of the height control assembly of the present invention;
fig. 8 shows a schematic diagram of the oscillating mechanism of the present invention in axial side view.
List of reference numerals
1. An outer protective case; 2. a slide control mechanism; 201. a baffle; 202. a control bracket; 203. a supporting rod A; 204. a connecting plate; 205. a pressing frame; 3. a straw assembly; 301. a top column; 302. a suction pipe; 303. a pull rod; 304. a plug plate; 305. a pull rope; 4. a suction control mechanism; 401. an outer branch box; 402. a support rod B; 403. a carriage; 404. a linkage shaft; 405. a driving shaft; 406. a winding wheel; 407. a control board; 5. a culture solution placement device; 501. a height control assembly; 5011. a supporting box; 5012. a screw rod; 5013. a master control rod; 5014. a carrying plate; 502. a strut C; 503. a sliding baffle; 504. a tension spring; 505. a push plate A; 6. a culture solution placing bottle; 7. an oscillating mechanism; 701. a rotating lever; 702. rotating the supporting frame; 703. a culture dish support plate; 704. an auxiliary pressing plate; 705. an elastic telescopic rod; 706. a linkage plate; 707. and a push plate B.
Detailed Description
Embodiments of the present invention are described in further detail below with reference to the accompanying drawings and examples.
Examples: please refer to fig. 1 to 8:
the invention provides a stem cell exosome culture device, which comprises: the device comprises an outer protective box 1, a sliding control mechanism 2, a suction pipe assembly 3, a suction control mechanism 4, a culture solution placement device 5, a culture solution placement bottle 6 and a vibration mechanism 7;
the whole outer protective box 1 is of a cuboid structure with a hollow inside, and an ultraviolet disinfection lamp is fixedly arranged in the outer protective box 1; one end of the bottom of the sliding control mechanism 2 is inserted into the outer protective box 1 in a sliding way; the top of the suction pipe assembly 3 is connected with the sliding control mechanism 2; the surface of the suction control mechanism 4 is fixedly connected with the top of the sliding control mechanism 2, and one end of the suction control mechanism 4 is connected with the suction pipe assembly 3; the top of the culture solution placement device 5 is inserted into the outer protective box 1; the culture solution placing bottle 6 is placed inside the culture solution placing device 5; the oscillating mechanism 7 is arranged inside the outer protective box 1, and the middle part of the oscillating mechanism 7 is contacted with the sliding control mechanism 2.
As shown in fig. 3, the slide control mechanism 2 includes: baffle 201, control bracket 202, strut a203, connecting plate 204 and press frame 205; the baffle 201 is of an H-shaped structure as a whole, and the baffle 201 is slidably arranged in the chute of the outer protective box 1; the whole control bracket 202 is of a U-shaped structure, the surface of the control bracket 202 is in sliding connection with the baffle 201, and the control bracket 202 can play a role in controlling the suction tube assembly 3 to be inserted into the culture solution placing bottle 6; the surface of the supporting rod A203 is provided with a spring, and the bottom of the supporting rod A203 is fixedly connected with the outer protective box 1 through a square plate; the whole connecting plate 204 is of an L-shaped structure, one end of the connecting plate 204 is fixedly connected with the baffle 201, the surface of the connecting plate 204 is connected with the support rod A203 in a sliding manner, and the connecting plate 204 can play a role in elastically supporting the control bracket 202; the whole press frames 205 are of L-shaped structures, the number of the press frames 205 is two, and one end of the top of each press frame 205 is fixedly connected with the control bracket 202.
As shown in fig. 4, the straw assembly 3 includes: top post 301, suction tube 302, pull rod 303, stopper 304 and pull cord 305; the whole top column 301 is of a circular tube structure, the top of the top column 301 is fixedly connected with the control bracket 202, and a spring is arranged in the top column 301; the top of the suction pipe 302 is fixedly connected with the jacking column 301; the pull rod 303 is slidably arranged inside the top column 301 and the suction pipe 302, and the top of the pull rod 303 is fixedly connected with the spring; the plug board 304 is fixedly arranged at the bottom of the pull rod 303, and the plug board 304 can control the suction pipe 302 to suck culture liquid; the bottom of the pull rope 305 is fixedly connected with the pull rod 303, and the top of the pull rope 305 slides through the control bracket 202 and is inserted into the suction control mechanism 4.
As shown in fig. 5, the suction control mechanism 4 includes: an outer strut box 401, a strut B402, a sliding frame 403 and a linkage shaft 404; the whole outer branch box 401 is of a long hair structure, and the surface of the outer branch box 401 is fixedly connected with the control bracket 202; the surface of the support rod B402 is provided with a spring, and the support rod B402 is fixedly arranged inside the outer support box 401; the rack plate is fixedly arranged on the surface of the sliding frame 403, the sliding frame 403 is of a T-shaped structure as a whole, and one end of the sliding frame 403 is connected with the support rod B402 in a sliding manner; the fixed gear that is provided with in universal driving shaft 404 surface, the inside at outer branch box 401 is rotationally installed to universal driving shaft 404, and the gear on universal driving shaft 404 surface is connected with the rack board meshing on carriage 403 surface, and the carriage 403 is slided and can be played the effect that drives universal driving shaft 404 and rotate.
As shown in fig. 5, the suction control mechanism 4 further includes: a driving shaft 405, a winding wheel 406 and a control board 407; the surface of the driving shaft 405 is fixedly provided with a gear, the driving shaft 405 is rotatably arranged in the outer support box 401, and the gear driving the surface of the driving shaft 405 is meshed with the linkage shaft 404; the winding wheel 406 is fixedly arranged in the middle of the driving shaft 405, the surface of the winding wheel 406 is connected with the other end of the pull rope 305, and the winding wheel 406 can play a role in assisting in winding the pull rope 305; one end of the control plate 407 is slidably inserted into the outer casing 401 and fixedly connected to the sliding frame 403.
As shown in fig. 6, the culture solution placement device 5 includes: a height control assembly 501, a strut C502, a sliding baffle 503, a tension spring 504 and a push plate A505; the whole height control assembly 501 is of an L-shaped structure, and the top of the height control assembly 501 is fixedly connected with the outer protective box 1; the support rod C502 is fixedly arranged inside the outer protective box 1; the sliding baffle 503 is connected with the two groups of struts C502 in a sliding way, and the sliding baffle 503 can play a role in assisting in sealing the culture solution placement bottle 6; the tension spring 504 is arranged on the surface of the support rod C502, one end of the tension spring 504 is fixedly connected with the inner wall of the outer protective box 1, and the other end of the tension spring 504 is fixedly connected with the sliding baffle 503; the whole push plate A505 is of a trapezoid structure, the push plate A505 is fixedly arranged on the surface of the sliding baffle 503, the pressing frame 205 is contacted with the inclined surface of the push plate A505, and the pressing frame 205 can play a role in assisting in controlling the sliding of the sliding baffle 503.
As shown in fig. 7, the height control assembly 501 further includes: a branch box 5011, a screw rod 5012, a main control rod 5013 and a bearing plate 5014; the whole branch box 5011 is of a cuboid structure with a hollow inside, a sliding groove is formed in the surface of the branch box 5011, and the top of the branch box 5011 is fixedly connected with the outer protective box 1; the screw rod 5012 is rotatably arranged in the branch box 5011; one end of a main control rod 5013 is inserted into the branch box 5011 in a rotating way, and one end of the main control rod 5013 is connected with the screw rod 5012 in a rotating way through a bevel gear; the whole T-shaped structure with the surface provided with a groove of the bearing plate 5014, one end of the bearing plate 5014 is inserted into the branch box 5011 in a sliding way and is in threaded connection with the screw rod 5012, and the bearing plate 5014 can play a role in supporting the culture solution placement bottle 6 in an auxiliary way.
As shown in fig. 8, the oscillating mechanism 7 includes: a rotating rod 701, a rotating bracket 702, a culture dish support plate 703 and an auxiliary pressing plate 704; the rotating rod 701 is rotatably installed inside the outer protective case 1; the whole rotating bracket 702 is of a V-shaped structure, and the bottom of the rotating bracket 702 is fixedly connected with the rotating rod 701; the bottom of the culture dish support plate 703 is fixedly connected with the rotating bracket 702, and the culture dish support plate 703 can play a role in bearing a culture dish; the whole auxiliary pressing plate 704 is of a right triangle structure, the number of the auxiliary pressing plates 704 is two, and the bottom of the auxiliary pressing plate 704 is fixedly connected with the culture dish supporting plate 703.
As shown in fig. 8, the oscillating mechanism 7 further includes: an elastic telescopic rod 705, a linkage plate 706 and a push plate B707; one end of the elastic telescopic rod 705 is fixedly connected with the inner wall of the outer protective box 1, the other end of the elastic telescopic rod 705 is contacted with the rotating support 702, and the elastic telescopic rod 705 can play a role in elastically supporting the rotating support 702 and the linkage plate 706; the surface of the linkage plate 706 is fixedly connected with the elastic telescopic rod 705, the bottom of the linkage plate 706 is in contact with the auxiliary pressing plate 704, and the linkage plate 706 can start the function of controlling the shaking of the culture dish support plate 703 and the auxiliary pressing plate 704; the push plate B707 is fixedly installed on the surface of the linkage plate 706, and the bottom of the press frame 205 is in contact with the linkage plate 706.
The invention also provides a stem cell exosome culture method, which completes the culture of stem cell exosome by the stem cell exosome culture device, and specifically comprises the following steps:
when the stem cell culture is carried out in work, a worker is required to extract culture solution to culture the stem cell, the worker places a culture dish on the surface of a culture dish support plate 703 in the outer protective box 1 and fixes the culture dish, then the worker places a culture solution placing bottle 6 which is required to be extracted in a groove of a bearing plate 5014, then a main control rod 5013 is rotated, one end of the main control rod 5013 controls a screw rod 5012 to rotate through a bevel gear assembly, the bearing plate 5014 slides upwards to push the top of the culture solution placing bottle 6 to be inserted into the outer protective box 1, at the moment, the top of the culture solution placing bottle 6 is contacted with a sliding baffle 503, and after the placement of the culture solution placing bottle 6 is completed; opening a protective door on one side of the outer protective box 1, fixedly placing a culture dish on the surface of a culture dish support plate 703, controlling a suction pipe assembly 3 at one end of the bottom to transversely or longitudinally slide by a worker through a control bracket 202, controlling the suction pipe 302 to be inserted into a culture solution placing bottle 6 to suck culture solution, pressing down the culture solution in the suction pipe 302, firstly, enabling a pressing frame 205 to be in slant contact with a push plate A505, enabling the push plate A505 to be stressed to slide to one side, further controlling a sliding baffle 503 to slide, opening a baffle plate at the top of the culture solution placing bottle 6, facilitating the insertion of the suction pipe 302 into the culture solution placing bottle 6, after the suction pipe 302 is inserted, controlling the sliding frame 403 to slide downwards by the worker through a control plate 407, controlling a rack plate 405 on the surface of the sliding frame 403 to rotate by a gear control driving shaft 405 on the surface of a linkage shaft 404, enabling a rolling wheel 406 to rotate by controlling a rolling wheel 406 to control a pull rope 305 to slide, further controlling the pull rod 303 and a plug plate 304 to slide, sucking the culture solution into the suction plate 302, then, controlling the assembly 3 to be pulled out by the culture solution placing bottle 505 through the control bracket 202, automatically pulling back a tension spring 504 at one end of the sliding baffle plate 503, automatically opening a top baffle plate 6, automatically controlling the culture solution placing bottle 205 to slide, alternately, controlling the suction plate 205 to slide to be in contact with the two sides of the culture dish 205B, and further, pressing the culture plate 706, and continuously moving the culture plate 706, and pressing the culture plate 706, and moving to slide, and pressing the culture plate 707 by the slide by the pulling plate, and pressing plate, and moving by the pulling plate 707, and pressing plate 707 by the pulling plate, and pressing plate, and moving when the culture plate, and pressing plate. The surface spring of the support rod B402 of the control board 407 is pushed back, the winding wheel 406 reversely rotates, the pulling force of the pull rope 305 is relieved, the spring at the top of the pull rod 303 is pushed back, and the liquid in the suction pipe 302 is pushed into a culture dish at the bottom of the culture dish support plate 703, so that the dilution process is completed.
The foregoing is merely exemplary embodiments of the present invention and is not intended to limit the scope of the invention, which is defined by the appended claims.
Claims (10)
1. A stem cell exosome culture apparatus, comprising: the device comprises an outer protective box (1), a sliding control mechanism (2), a suction pipe assembly (3), a suction control mechanism (4), a culture solution placement device (5), a culture solution placement bottle (6) and a vibration mechanism (7);
the whole outer protective box (1) is of a cuboid structure with a hollow inside, and an ultraviolet disinfection lamp is fixedly arranged in the outer protective box (1); one end of the bottom of the sliding control mechanism (2) is inserted into the outer protective box (1) in a sliding way; the top of the suction pipe assembly (3) is connected with the sliding control mechanism (2); the surface of the suction control mechanism (4) is fixedly connected with the top of the sliding control mechanism (2), and one end of the suction control mechanism (4) is connected with the suction pipe assembly (3); the top of the culture solution placement device (5) is inserted into the outer protective box (1); the culture solution placing bottle (6) is placed inside the culture solution placing device (5); the vibration mechanism (7) is arranged inside the outer protective box (1), and the middle part of the vibration mechanism (7) is contacted with the sliding control mechanism (2).
2. The stem cell exosome culture apparatus according to claim 1, wherein: the slide control mechanism (2) includes: a baffle (201), a control bracket (202), a support rod A (203), a connecting plate (204) and a pressing frame (205); the baffle (201) is of an H-shaped structure as a whole, and the baffle (201) is slidably arranged in a chute of the outer protective box (1); the control bracket (202) is of a U-shaped structure as a whole, and the surface of the control bracket (202) is in sliding connection with the baffle (201); the surface of the supporting rod A (203) is provided with a spring, and the bottom of the supporting rod A (203) is fixedly connected with the outer protective box (1) through a square plate; the whole connecting plate (204) is of an L-shaped structure, one end of the connecting plate (204) is fixedly connected with the baffle (201), and the surface of the connecting plate (204) is connected with the support rod A (203) in a sliding manner; the whole press frames (205) are of L-shaped structures, the number of the press frames (205) is two, and one end of the top of each press frame (205) is fixedly connected with the control bracket (202).
3. A stem cell exosome culture apparatus according to claim 2, wherein: the suction tube assembly (3) comprises: a top column (301), a suction pipe (302), a pull rod (303), a plug board (304) and a pull rope (305); the whole top column (301) is of a circular tube structure, the top of the top column (301) is fixedly connected with the control bracket (202), and a spring is arranged in the top column (301); the top of the suction pipe (302) is fixedly connected with the jacking column (301); the pull rod (303) is slidably arranged in the jacking column (301) and the suction pipe (302), and the top of the pull rod (303) is fixedly connected with the spring; the plug plate (304) is fixedly arranged at the bottom of the pull rod (303); the bottom of the stay cord (305) is fixedly connected with the pull rod (303), and the top of the stay cord (305) slides through the control bracket (202) and is inserted into the suction control mechanism (4).
4. A stem cell exosome culture apparatus according to claim 3, wherein: the suction control mechanism (4) includes: an outer support box (401), a support rod B (402), a sliding frame (403) and a linkage shaft (404); the whole outer branch box (401) is of a long hair structure, and the surface of the outer branch box (401) is fixedly connected with the control bracket (202); the surface of the support rod B (402) is provided with a spring, and the support rod B (402) is fixedly arranged in the outer support box (401); the rack plate is fixedly arranged on the surface of the sliding frame (403), the sliding frame (403) is of a T-shaped structure as a whole, and one end of the sliding frame (403) is connected with the support rod B (402) in a sliding manner; the surface of the linkage shaft (404) is fixedly provided with a gear, the linkage shaft (404) is rotatably arranged inside the outer support box (401), and the gear on the surface of the linkage shaft (404) is meshed and connected with a rack plate on the surface of the sliding frame (403).
5. The stem cell exosome culture apparatus according to claim 4, wherein: the suction control mechanism (4) further comprises: a driving shaft (405), a winding wheel (406) and a control board (407); the surface of the driving shaft (405) is fixedly provided with a gear, the driving shaft (405) is rotatably arranged in the outer supporting box (401), and the gear on the surface of the driving shaft (405) is meshed and connected with the linkage shaft (404); the winding wheel (406) is fixedly arranged in the middle of the driving shaft (405), and the surface of the winding wheel (406) is connected with the other end of the pull rope (305); one end of the control board (407) is inserted into the outer branch box (401) in a sliding way and is fixedly connected with the sliding frame (403).
6. The stem cell exosome culture apparatus according to claim 1, wherein: the culture solution placement device (5) comprises: the device comprises a height control assembly (501), a support rod C (502), a sliding baffle (503), a tension spring (504) and a push plate A (505); the top of the height control component (501) is fixedly connected with the outer protective box (1); the support rod C (502) is fixedly arranged in the outer protective box (1); the sliding baffle (503) is connected with the two groups of support rods C (502) in a sliding way; the tension spring (504) is arranged on the surface of the support rod C (502), one end of the tension spring (504) is fixedly connected with the inner wall of the outer protective box (1), and the other end of the tension spring (504) is fixedly connected with the sliding baffle (503); the whole push plate A (505) is of a trapezoid structure, and the push plate A (505) is fixedly arranged on the surface of the sliding baffle (503).
7. The stem cell exosome culture apparatus according to claim 6, wherein: the height control assembly (501) further comprises: the device comprises a supporting box (5011), a screw rod (5012), a main control rod (5013) and a bearing plate (5014); the whole support box (5011) is of a cuboid structure with a hollow inside, a sliding groove is formed in the surface of the support box (5011), and the top of the support box (5011) is fixedly connected with the outer protective box (1); the screw rod (5012) is rotatably arranged in the branch box (5011); one end of a main control rod (5013) is inserted into the branch box (5011) in a rotating way, and one end of the main control rod (5013) is connected with the screw rod (5012) in a rotating way through a bevel gear; the whole bearing plate (5014) is of a T-shaped structure with a groove formed in the surface, and one end of the bearing plate (5014) is inserted into the supporting box (5011) in a sliding mode and is in threaded connection with the screw rod (5012).
8. A stem cell exosome culture apparatus according to claim 2, wherein: the oscillation mechanism (7) comprises: a rotating rod (701), a rotating bracket (702), a culture dish support plate (703) and an auxiliary pressing plate (704); the rotating rod (701) is rotatably arranged inside the outer protective box (1); the whole rotating support (702) is of a V-shaped structure, and the bottom of the rotating support (702) is fixedly connected with the rotating rod (701); the bottom of the culture dish support plate (703) is fixedly connected with the rotating support frame (702); the whole auxiliary pressing plates (704) are of right triangle structures, the number of the auxiliary pressing plates (704) is two, and the bottoms of the auxiliary pressing plates (704) are fixedly connected with the culture dish support plates (703).
9. The stem cell exosome culture apparatus according to claim 8, wherein: the oscillating mechanism (7) further comprises: an elastic telescopic rod (705), a linkage plate (706) and a push plate B (707); one end of an elastic telescopic rod (705) is fixedly connected with the inner wall of the outer protective box (1), and the other end of the elastic telescopic rod (705) is contacted with the rotating bracket (702); the surface of the linkage plate (706) is fixedly connected with the elastic telescopic rod (705), and the bottom of the linkage plate (706) is contacted with the auxiliary pressing plate (704); the push plate B (707) is fixedly arranged on the surface of the linkage plate (706), and the bottom of the pressing frame (205) is contacted with the linkage plate (706).
10. A method for culturing stem cell exosomes is characterized in that the culture of the stem cell exosomes is completed by the stem cell exosomes culture device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310855932.3A CN117025380A (en) | 2023-07-13 | 2023-07-13 | Stem cell exosome culture device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310855932.3A CN117025380A (en) | 2023-07-13 | 2023-07-13 | Stem cell exosome culture device and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117025380A true CN117025380A (en) | 2023-11-10 |
Family
ID=88601315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310855932.3A Withdrawn CN117025380A (en) | 2023-07-13 | 2023-07-13 | Stem cell exosome culture device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117025380A (en) |
-
2023
- 2023-07-13 CN CN202310855932.3A patent/CN117025380A/en not_active Withdrawn
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Application publication date: 20231110 |