CN1170156C - Integrated mini capillary electrophoresis chip in non-valve pump type - Google Patents
Integrated mini capillary electrophoresis chip in non-valve pump type Download PDFInfo
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- CN1170156C CN1170156C CNB03115512XA CN03115512A CN1170156C CN 1170156 C CN1170156 C CN 1170156C CN B03115512X A CNB03115512X A CN B03115512XA CN 03115512 A CN03115512 A CN 03115512A CN 1170156 C CN1170156 C CN 1170156C
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- capillary electrophoresis
- valveless pump
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Abstract
The present invention relates to an integrated miniature capillary electrophoresis chip in a non-valve pump type, which is formed by the bonding of two blocks of base-body materials. A miniature non-valve pump, an inlet flow channel and an outlet flow channel are arranged in a composition surface. An electrophoretic separation flow channel and an electrophoresis sample flow channel are mutually cross, and one end of the electrophoresis sample flow channel is connected with the inlet flow channel to form a T shape. The miniature non-valve pump is utilized to be used as an automatic sample change device for continuously detecting multi-sample electrophoresis. The automatic sample change device discharges the original sample to a waste liquid tank after the former sample finishes detecting, and a new sample is conveyed to a sample inlet point simultaneously. The integrated miniature capillary electrophoresis chip has the advantages of repeated use, simplified detection equipment, shortened continuous detection time of multiple samples, detection cost reduction and low operation requirements for working personnel.
Description
Technical field
The present invention relates to biochemical detection device, particularly a kind of integrated micro valveless pump formula capillary electrophoresis chip.
Background technology
Capillary Electrophoresis (Capillary Electrophoresis, be called for short CE) be the separate analytical technique that a kind of novel electrophoresis that develops rapidly the beginning of the eighties combines with chromatogram, have the analysis efficiency height, characteristics such as analysis speed is fast, amount of samples is few, applied range, become the important compartment analysis means nineties, had good application prospects in fields such as biology, chemistry, medicine, environmental protection, food.The capillary electrophoresis chip technology that develops rapidly after the nineties is to be technical backstopping with the micro fabrication, with crystalline silicon, glass, plastics (mainly being organic glass), pottery and silicon rubber is matrix material, by capillary electrophoresis technique, with process integration such as sample feeding, reaction, separation, detection to together multifunction efficient, fast, microscale experiment chamber technology that sample dosage is few.Up to now, the separation that is used for a series of chemical substances that the capillary electrophoresis chip technology is successful, especially in biochemical field, successful carrying out the separation detection of determined dna sequence, polypeptide and protein, amino acid, unicellular and unimolecule etc.But still there is deficiency in the automaticity of this technology in testing process, is mainly reflected in following 2 points:
(1) in several samples continuous detecting process, after a kind of sample detection finishes, capillary electrophoresis chip must be taken off from pick-up unit, and adopt the manual mode of operation that raw sample residual in the chip is cleaned up and carry out the chip pre-service for next sample, the time that is spent is long.Simultaneously, after changing new sample again, chip also needs to reorientate with pick-up unit, because capillary electrophoresis tube road size is about tens microns, therefore reorientating also needs experience to require and the regular hour, and the repeated variation of twice detection influenced accuracy of detection before and after resetting also can cause.So, adopt manually to vary the speed and the accuracy of detection of various product continuous detecting are brought influence, especially be embodied on the long problem that expends time in.
(2) present, most of capillary electrophoresis chips all are disposable, have bigger waste, and it is higher to detect cost.So, develop reusable novel capillary electrophoresis chip and will play great prograding for the commercialization process of capillary electrophoresis chip.
Summary of the invention
The purpose of this invention is to provide a kind of have vary and cleaning function and reusable integrated micro valveless pump formula capillary electrophoresis chip automatically.
The technical solution used in the present invention is as follows, and it comprises:
1) forms by first block matrix material and the second block matrix material bonding, on first block matrix material, be equipped with by cavity, drive the micro valveless pump that film is formed, in second block matrix material, be etched with inlet channel and the outlet flow that lays respectively at the cavity both sides on the composition surface, one termination sample introduction groove of inlet channel, the trapezoidal inlet channel of the other end through progressively enlarging is communicated with the entrance point of valveless pump, one termination waste liquid tank of outlet flow, the trapezoidal outlet flow of the other end through progressively dwindling is communicated with the endpiece of valveless pump, the characteristic plane of two trapezoidal import and export runners and cavity plane parallel, electrophoretic separation runner and electrophoresis sample introduction runner intersect and are communicated with, another waste liquid tank of electrophoretic separation runner one termination, another termination buffering liquid groove, electrophoresis sample introduction runner one end becomes " T " font to be communicated with another termination the 3rd waste liquid tank with inlet channel.
2) the electrophoretic separation runner is identical with the sectional dimension of electrophoresis sample introduction runner, and with the ratio of the sectional area of inlet channel less than 1: 10.
The present invention compares with background technology, and the useful effect that has is:
1) integrated micro valveless pump formula capillary electrophoresis chip provided by the invention, micro valveless pump is realized fast automatic varying in various product continuous detecting, be expelled to waste liquid tank at fresh sample original sample when the sample introduction groove pumps into from sample delivery point, and the logical gas column that pumps into one centimetre of left and right sides length is to isolate two kinds of different samples between two kinds of samples, and on average each sample detection time reduced to 2 minutes from original 15 minutes;
2) micro valveless pump formula capillary electrophoresis chip provided by the invention, micro valveless pump can pump into cleaning fluid and clean sample introduction groove, sample delivery point and Micropump system after all samples detection finishes, need not artificial cleaning;
3) the present invention utilizes micropump inlet channel and electrophoresis sample introduction runner to be " T " font to be connected, and the version that differs greatly of two cross section of fluid channel sizes, has effectively avoided the influence of micro valveless pump pressure surge to test sample in the electrophoresis runner;
4) micro valveless pump formula capillary electrophoresis chip provided by the invention is reusable, has simplified checkout equipment, shortened the several samples continuous detecting time, has reduced to detect cost and to staff's operation requirement.
Description of drawings
Fig. 1 is a structural principle synoptic diagram of the present invention;
Fig. 2 is the sectional view on the A-A plane of Fig. 1.
Embodiment
Describe the specific embodiment of the present invention in detail below in conjunction with drawings and Examples.
As Fig. 1, shown in Figure 2, the present invention includes: form by the first block matrix material A and the second block matrix material B bonding, on the first block matrix material A, be equipped with by cavity 5, drive the micro valveless pump that film 6 is formed, in the second block matrix material B, be etched with inlet channel 3 and the outlet flow 8 that lays respectively at cavity 5 both sides on the composition surface, one termination sample introduction groove 1 of inlet channel 3, the trapezoidal inlet channel 4 of the other end through progressively enlarging is communicated with the entrance point of valveless pump, one termination waste liquid tank 9 of outlet flow 8, the trapezoidal outlet flow 7 of the other end through progressively dwindling is communicated with the endpiece of valveless pump, the characteristic plane of two trapezoidal import and export runners and cavity 5 plane parallel, electrophoretic separation runner 10 and electrophoresis sample introduction runner 13 intersect and are communicated with, another waste liquid tank 11 of electrophoretic separation runner 10 1 terminations, another termination buffering liquid groove 14, electrophoresis sample introduction runner 13 1 ends are communicated with another termination the 3rd waste liquid tank 12 with 3 one-tenth of inlet channel " T " font.
The size of said trapezoidal inlet channel 4 and trapezoidal outlet flow 7 is identical.
Said electrophoretic separation runner 10 is identical with the sectional dimension of electrophoresis sample introduction runner 13, and with the ratio of the sectional area of inlet channel 3 less than 1: 10.
Said micro valveless pump film 6 is a mini type mechanical double vibrations film, and vibration frequency range is 0-100Hz.
Said matrix material is organic glass or crystalline silicon or or pottery or silicon rubber glass.
Micro valveless pump is integrated on the capillary electrophoresis chip as varying power source, when original sample test finishes, in the time of need changing to fresh sample, start micro valveless pump, the air gas column that pumps into one centimetre of left and right sides length enters micro valveless pump inlet channel 3, two kinds of samples before and after the micro-gas column of this section is used for isolating, add fresh sample again at sample introduction groove 1 place after feeding gas column, micro valveless pump also is delivered to fresh sample sample delivery point 2 when original sample is evacuated to waste liquid tank 9 by sample introduction groove 1, micro valveless pump work was closed after 30 seconds, carry out capillary electrophoresis analysis then, at first sample introduction groove 1 and the waste liquid tank 12 at electrophoresis sample introduction runner two ends adds sample introduction voltage, make sample enter the infall of electrophoresis sample introduction runner 13 and electrophoretic separation runner 10 by sample delivery point 2, then sample introduction voltage is closed, and add separation voltage at the waste liquid tank 14 at electrophoretic separation runner 10 two ends and waste liquid tank 11 and carry out sample separation and detect.After all samples test finishes, 1 adds cleaning fluid in the sample introduction groove, the startup Micropump cleans up the sample of sample introduction groove 1, sample delivery point 2, micro valveless pump inlet channel 3, the trapezoidal inlet channel 4 of micro valveless pump, micro valveless pump cavity 5, micro valveless pump exit cone 7, the trapezoidal outlet flow 8 of micro valveless pump, the cleaning of the residual sample in electrophoresis sample channel 13 and the electrophoretic separation pipeline 10 is then finished by the electrophoresis mode, so just can make integrated micro valveless pump formula capillary electrophoresis chip realize reusing.
Claims (4)
1, a kind of integrated micro valveless pump formula capillary electrophoresis chip is characterized in that it comprises:
1) forms by first block matrix material (A) and second block matrix material (B) bonding, on first block matrix material (A), be equipped with by cavity (5), drive the micro valveless pump that film (6) is formed, in second block matrix material (B), be etched with the inlet channel (3) and the outlet flow (8) that lay respectively at cavity (5) both sides on the composition surface, one termination sample introduction groove (1) of inlet channel (3), the trapezoidal inlet channel (4) of the other end through progressively enlarging is communicated with the entrance point of valveless pump, one termination waste liquid tank (9) of outlet flow (8), the trapezoidal outlet flow (7) of the other end through progressively dwindling is communicated with the endpiece of valveless pump, the characteristic plane of two trapezoidal import and export runners and cavity (5) plane parallel, electrophoretic separation runner (10) and electrophoresis sample introduction runner (13) intersect and are communicated with, electrophoretic separation runner (10) one another waste liquid tanks of termination (11), another termination buffering liquid groove (14), electrophoresis sample introduction runner (13) one ends become " T " font to be communicated with another termination the 3rd waste liquid tank (12) with inlet channel (3);
2) electrophoretic separation runner (10) is identical with the sectional dimension of electrophoresis sample introduction runner (13), and with the ratio of the sectional area of inlet channel (3) less than 1: 10.
2, a kind of integrated micro valveless pump formula capillary electrophoresis chip according to claim 1, it is characterized in that: the size of said trapezoidal inlet channel (4) and trapezoidal outlet flow (7) is identical.
3, a kind of integrated micro valveless pump formula capillary electrophoresis chip according to claim 1, it is characterized in that: said micro valveless pump film (6) is a mini type mechanical double vibrations film, and vibration frequency range is 0-100Hz.
4, a kind of integrated micro valveless pump formula capillary electrophoresis chip according to claim 1 is characterized in that: said matrix material is organic glass or crystalline silicon or glass or pottery or silicon rubber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB03115512XA CN1170156C (en) | 2003-02-22 | 2003-02-22 | Integrated mini capillary electrophoresis chip in non-valve pump type |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB03115512XA CN1170156C (en) | 2003-02-22 | 2003-02-22 | Integrated mini capillary electrophoresis chip in non-valve pump type |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1431495A CN1431495A (en) | 2003-07-23 |
| CN1170156C true CN1170156C (en) | 2004-10-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB03115512XA Expired - Fee Related CN1170156C (en) | 2003-02-22 | 2003-02-22 | Integrated mini capillary electrophoresis chip in non-valve pump type |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100356061C (en) * | 2006-02-14 | 2007-12-19 | 南京航空航天大学 | Ribbed miniature no-valve pump |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425983C (en) * | 2003-08-29 | 2008-10-15 | 中国科学院生态环境研究中心 | Laser induced fluorescence and light absorption bifunction detecting microflow controlled electrophoresis ehip |
| CN100443397C (en) * | 2004-05-28 | 2008-12-17 | 哈尔滨工业大学 | Microcooling observing and controlling system and machining method thereof |
| CN1313355C (en) * | 2005-06-09 | 2007-05-02 | 上海交通大学 | Single piece of pneumatic gelatious tiny valve |
| CN1313356C (en) * | 2005-06-09 | 2007-05-02 | 上海交通大学 | Micro oxygen pump based on hydrogen peroxide solution decomposition |
| CN104677972B (en) * | 2015-02-10 | 2017-02-22 | 四川大学 | Constant-speed micro-channel capillary electrophoresis chip |
| CN113413936B (en) * | 2021-08-24 | 2021-11-30 | 北京寻因生物科技有限公司 | Flow regulation structure and microfluid chip |
-
2003
- 2003-02-22 CN CNB03115512XA patent/CN1170156C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100356061C (en) * | 2006-02-14 | 2007-12-19 | 南京航空航天大学 | Ribbed miniature no-valve pump |
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| Publication number | Publication date |
|---|---|
| CN1431495A (en) | 2003-07-23 |
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