CN116998727A - Small-molecule oligopeptide powder composition, and preparation method and application thereof - Google Patents
Small-molecule oligopeptide powder composition, and preparation method and application thereof Download PDFInfo
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- CN116998727A CN116998727A CN202311114914.6A CN202311114914A CN116998727A CN 116998727 A CN116998727 A CN 116998727A CN 202311114914 A CN202311114914 A CN 202311114914A CN 116998727 A CN116998727 A CN 116998727A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 150000003384 small molecules Chemical class 0.000 title claims description 12
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- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 31
- 241000283690 Bos taurus Species 0.000 claims abstract description 29
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- 108010084695 Pea Proteins Proteins 0.000 claims abstract description 11
- 235000019702 pea protein Nutrition 0.000 claims abstract description 11
- 230000036039 immunity Effects 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 8
- 230000002708 enhancing effect Effects 0.000 claims abstract description 7
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- 239000007788 liquid Substances 0.000 claims description 38
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 35
- 235000015278 beef Nutrition 0.000 claims description 30
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 30
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- 238000001914 filtration Methods 0.000 claims description 25
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- 239000000440 bentonite Substances 0.000 claims description 20
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- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 15
- 108090000145 Bacillolysin Proteins 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
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- 102000035092 Neutral proteases Human genes 0.000 claims description 10
- 239000011246 composite particle Substances 0.000 claims description 10
- 238000005238 degreasing Methods 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 239000008213 purified water Substances 0.000 claims description 10
- 238000002791 soaking Methods 0.000 claims description 10
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- 235000005979 Citrus limon Nutrition 0.000 claims description 5
- 244000131522 Citrus pyriformis Species 0.000 claims description 5
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims description 5
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- 235000009685 Crataegus X maligna Nutrition 0.000 claims description 5
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- 235000009486 Crataegus bullatus Nutrition 0.000 claims description 5
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims description 5
- 235000009682 Crataegus limnophila Nutrition 0.000 claims description 5
- 240000000171 Crataegus monogyna Species 0.000 claims description 5
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- 240000001811 Dioscorea oppositifolia Species 0.000 claims description 5
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- 235000016623 Fragaria vesca Nutrition 0.000 claims description 5
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- 235000011363 Fragaria x ananassa Nutrition 0.000 claims description 5
- 108090000526 Papain Proteins 0.000 claims description 5
- 244000300264 Spinacia oleracea Species 0.000 claims description 5
- 235000009337 Spinacia oleracea Nutrition 0.000 claims description 5
- 102000004142 Trypsin Human genes 0.000 claims description 5
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- 238000004440 column chromatography Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 235000019425 dextrin Nutrition 0.000 claims description 5
- 239000003248 enzyme activator Substances 0.000 claims description 5
- 239000000686 essence Substances 0.000 claims description 5
- 108010007119 flavourzyme Proteins 0.000 claims description 5
- 235000003599 food sweetener Nutrition 0.000 claims description 5
- 230000002779 inactivation Effects 0.000 claims description 5
- FYDKNKUEBJQCCN-UHFFFAOYSA-N lanthanum(3+);trinitrate Chemical compound [La+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O FYDKNKUEBJQCCN-UHFFFAOYSA-N 0.000 claims description 5
- 235000013372 meat Nutrition 0.000 claims description 5
- 230000007935 neutral effect Effects 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- 239000001814 pectin Substances 0.000 claims description 5
- 229920001277 pectin Polymers 0.000 claims description 5
- 235000010987 pectin Nutrition 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 239000001509 sodium citrate Substances 0.000 claims description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- UGTZMIPZNRIWHX-UHFFFAOYSA-K sodium trimetaphosphate Chemical compound [Na+].[Na+].[Na+].[O-]P1(=O)OP([O-])(=O)OP([O-])(=O)O1 UGTZMIPZNRIWHX-UHFFFAOYSA-K 0.000 claims description 5
- 239000003765 sweetening agent Substances 0.000 claims description 5
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- 238000000108 ultra-filtration Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 16
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000035764 nutrition Effects 0.000 abstract description 5
- 208000001132 Osteoporosis Diseases 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
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- 206010067484 Adverse reaction Diseases 0.000 abstract description 2
- 206010033425 Pain in extremity Diseases 0.000 abstract description 2
- 230000006838 adverse reaction Effects 0.000 abstract description 2
- 230000001502 supplementing effect Effects 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 238000000605 extraction Methods 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000208340 Araliaceae Species 0.000 description 3
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 description 3
- 235000008434 ginseng Nutrition 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108010051873 alkaline protease Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000007661 gastrointestinal function Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/01—Instant products; Powders; Flakes; Granules
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a small molecular oligopeptide powder composition, a preparation method and application thereof, wherein the composition comprises 58-72% of bovine bone collagen oligopeptide powder, 20-33% of spirulina oligopeptide, 15-30% of pea protein peptide powder, 2-5% of flavoring agent, 1-3% of medicine and food homologous additive powder and 1-5% of fruit and vegetable powder. The bovine collagen oligopeptide composition has more comprehensive nutrition and better effect, the compound use of the product has a synergistic effect, the effect of enhancing immunity is far greater than the superposition of the effects of single dose, the technical problem that the existing osteoporosis treatment method can produce adverse reaction is solved, the effects of obviously improving osteoporosis, supplementing bone nutrition, relieving leg pain and enhancing immunity are produced; and the processing is simple, the cost is low, the food can be eaten as daily staple food for people, and has the technical effects of no toxic or side effect and obvious economic benefit of enterprises.
Description
Technical Field
The invention relates to the technical field of human health products, in particular to a small molecular oligopeptide powder composition, a preparation method and application thereof.
Background
The small molecular peptide is a classification of polypeptide, the molecular weight section is generally below 1500 daltons, and is also called small peptide, oligopeptide or small molecular active peptide, and generally consists of 2-6 amino acids, compared with the protein with larger molecular weight, the small molecular peptide has the advantages of no need of digestion and direct absorption, no need of consuming human energy during absorption and no increase of gastrointestinal function burden of human body, and in addition, the small molecular peptide has the function of a carrier, and can carry other nutrition in the human body on the body and into cells and tissues of the human body, so that the small molecular peptide is receiving more and more attention.
Bovine bone mainly contains collagen, and has the effects of high nutritive value, bone strengthening and human immunity improving. Bovine bone enters human body usually in a manner of eating or decocting, collagen is generally digested into oligopeptide or amino acid by digestive juice to be absorbed and utilized by the organism, and the people with low immunity have common weakness of spleen and stomach or gastrointestinal tract reaction, so that the activity of digestive tract enzymes is low and the digestion capacity is poor, thus the administration can not only cause the burden of the digestive system of a patient, but also can not achieve the proper efficacy, and the small molecular oligopeptide obtained by treating bovine collagen can effectively overcome the problems. The existing extraction process of the small molecular oligopeptide has the defects of low protein extraction efficiency, high impurity content in the extracted small molecular peptide and wide molecular weight distribution of products, and the types of proteins contained in different substances are different, so that the extraction process of the small molecular peptide of different substances has little reference significance.
Therefore, the invention provides a small molecular oligopeptide with high product quality, which is suitable for industrial production, and a preparation method thereof, and the technical problem to be solved by the technicians in the field is urgent.
Disclosure of Invention
Aiming at the defects pointed out by the background technology, the invention aims to provide the bovine bone collagen oligopeptide powder composition capable of enhancing the immunity, and the preparation method and the application thereof.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a small molecular oligopeptide powder composition comprises 58-72% of bovine collagen oligopeptide powder, 20-33% of spirulina oligopeptide, 15-30% of pea protein peptide powder, 2-5% of flavoring agent, 1-3% of medicine and food homologous additive powder and 1-5% of fruit and vegetable powder.
Further, the preparation method of the bovine collagen oligopeptide powder comprises the following steps:
(1) And (3) raw material treatment: removing fat, residual meat and the like on the surface of fresh beef bones, crushing the beef bones to 2-3 cm of bone blocks by using a bone crusher, washing the crushed beef bones with clear water until the pH value is neutral, putting the beef bones into a cooking pot, and adding water for cooking to obtain cooking liquid;
(2) Degreasing: immersing the cooking liquid into ethyl acetate for soaking and degreasing, standing, filtering and separating to obtain degreased beef bone liquid; adding defluorinated composite particles, and filtering and separating to obtain defluorinated suspension;
(3) Enzymolysis: adding complex enzyme into the suspension, adding citric acid to adjust the pH value to 5-7.5, and performing enzymolysis for 1-2h in a constant temperature environment of 35-45 ℃ to obtain beef bone enzymolysis solution; the complex enzyme consists of 35wt% of papain, 30% of alkaline protease, 30wt% of trypsin, 17wt% of flavourzyme and 3% of enzyme activator;
(4) Sequentially performing ultrafiltration and gel column chromatography purification to obtain the bovine bone small molecular peptide.
Further, in the step (2), the preparation method of the defluorinated composite particles comprises the following steps:
adding carboxymethyl chitosan into water to prepare carboxymethyl chitosan water solution with the concentration of 1-5 wt%; adding bentonite into carboxymethyl chitosan water solution, ultrasonically stirring for 4-8h, filtering to separate out bentonite, adding bentonite into sodium trimetaphosphate water solution, soaking for 1-3h, filtering to separate out bentonite, and drying to obtain modified bentonite; adding the modified bentonite into ethanol solution of lanthanum nitrate, stirring and reacting for 2-5h, filtering, washing and drying to obtain the modified bentonite;
further, the flavoring agent includes sodium citrate/citric acid, salt, pectin, flavoring essence, sweetener and dextrin mixed in any ratio.
Further, the preparation method of the spirulina oligopeptide comprises the following steps: (1) Slicing Ginseng radix, adding purified water into spirulina powder, stirring, and decocting to obtain decoction mixture;
(2) Cooling the decoction mixture to 58-58 ℃, adding cellulase, amylase and saccharifying enzyme, stirring and performing enzymolysis to obtain a first enzymolysis liquid;
(3) Centrifuging and separating the first enzymolysis liquid to obtain desugared spirulina powder, adding purified water into the desugared spirulina powder, heating to 50-58 ℃, adding exo-aspergillus oryzae neutral protease and alkaline protease, and stirring for enzymolysis for 3-4 hours to obtain a second enzymolysis liquid; wherein the addition of the exo-aspergillus oryzae neutral protease is 0.5-0.8% of the weight of the raw material, and the addition of the alkaline protease is 0.1-0.3% of the weight of the raw material;
(4) And heating the second enzymolysis liquid to raise the temperature for enzyme inactivation, and separating and purifying to obtain the spirulina oligopeptide.
The invention also discloses a preparation method of the small molecule oligopeptide powder composition, which comprises the following steps:
(a) Selecting components with granularity of 70 meshes or 150 meshes, and respectively weighing the components according to the proportion of the components for later use;
(b) Uniformly mixing bovine collagen oligopeptide, spirulina oligopeptide, pea protein peptide powder and flavoring agent to obtain prepared powder;
(c) Uniformly mixing the Chinese yam, the hawthorn and the angelica dahurica to obtain medicinal and edible additive powder; uniformly mixing apple powder, strawberry powder, lemon powder, spinach powder and carrot powder to obtain fruit and vegetable powder;
(d) Adding the medicinal and edible additive powder and the fruit and vegetable powder obtained in the step (c) into the prepared powder in the step (b), and uniformly mixing to obtain the micromolecular oligopeptide powder composition.
The invention also discloses application of the small molecular oligopeptide powder composition in preparing a product for improving human immunity.
Compared with the prior art, the invention has the beneficial effects that:
(1) The bovine bone collagen oligopeptide composition has small molecular weight, wherein the molecular weight of the oligopeptide is more than 92 percent of the total weight of the oligopeptide less than 1500Da, and the oligopeptide is well absorbed, so that the bovine bone collagen oligopeptide composition can be absorbed by most of human bodies, and the effect of enhancing the immunity is achieved;
(2) The bovine collagen oligopeptide composition has more comprehensive nutrition and better effect, the compound use of the product has a synergistic effect, the effect of enhancing immunity is far greater than the superposition of the effects of single dose, the technical problem that the existing osteoporosis treatment method can produce adverse reaction is solved, the effects of obviously improving osteoporosis, supplementing bone nutrition, relieving leg pain and enhancing immunity are produced; the processing is simple, the cost is low, the food can be eaten as daily staple food for people, and the food has no toxic or side effect and obvious technical effect on economic benefit of enterprises;
(3) According to the invention, the ginseng is subjected to enzymolysis by using cellulase, amylase and saccharifying enzyme, so that the cell wall is effectively disintegrated, the dissolution of intracellular proteins is promoted, cellulose, starch and saccharide substances in the ginseng can be hydrolyzed into soluble substances, the purpose of separating the proteins from the raw materials can be achieved by simple centrifugal separation, and the combination of three non-protease enzymes can improve the enzymolysis rate, reduce the consumption of protease and improve the extraction purity of the proteins; then adding compound enzyme of external Aspergillus oryzae neutral proteinase and alkaline proteinase to make secondary enzymolysis, and making 2 enzymes implement synergistic action, so that it can implement enzyme cutting of different sites and broad-spectrum property of spirulina protein and long-chain polypeptide to obtain the spirulina peptide with concentrated molecular weight distribution, and can raise extraction rate of spirulina peptide, and its extraction rate (spirulina peptide content of total protein in spirulina) can be up to above 92%, its relative molecular mass can be up to above 91% in the extracted polypeptide, and its molecular weight can be up to above 60% in the extracted polypeptide, and its content is high, and can be easily digested and absorbed, so that it can obviously raise utilization rate of spirulina protein.
Detailed Description
The invention is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Examples
A small molecular oligopeptide powder composition comprises 58% of bovine collagen oligopeptide powder, 33% of spirulina oligopeptide, 5% of pea protein peptide powder, 2% of flavoring agent, 2% of medicinal and edible additive powder and 1% of fruit and vegetable powder.
Further, the preparation method of the bovine collagen oligopeptide powder comprises the following steps:
(1) And (3) raw material treatment: removing fat, residual meat and the like on the surface of fresh beef bones, crushing the beef bones to 2-3 cm of bone blocks by using a bone crusher, washing the crushed beef bones with clear water until the pH value is neutral, putting the beef bones into a cooking pot, and adding water for cooking to obtain cooking liquid;
(2) Degreasing: immersing the cooking liquid into ethyl acetate for soaking and degreasing, standing, filtering and separating to obtain degreased beef bone liquid; adding defluorinated composite particles, and filtering and separating to obtain defluorinated suspension;
(3) Enzymolysis: adding complex enzyme into the suspension, adding citric acid to adjust the pH value to 5, and carrying out enzymolysis for 2 hours in a constant temperature environment at 35 ℃ to obtain beef bone enzymolysis liquid; the complex enzyme consists of 35wt% of papain, 30% of alkaline protease, 30wt% of trypsin, 17wt% of flavourzyme and 3% of enzyme activator;
(4) Sequentially performing ultrafiltration and gel column chromatography purification to obtain the bovine bone small molecular peptide.
Further, in the step (2), the preparation method of the defluorinated composite particles comprises the following steps:
adding carboxymethyl chitosan into water to prepare carboxymethyl chitosan water solution with the concentration of 5 wt%; adding bentonite into carboxymethyl chitosan water solution, ultrasonically stirring for 8 hours, filtering to separate out bentonite, adding bentonite into sodium trimetaphosphate water solution, soaking for 3 hours, filtering to separate out bentonite, and drying to obtain modified bentonite; adding the modified bentonite into an ethanol solution of lanthanum nitrate, stirring and reacting for 5 hours, filtering, washing and drying to obtain the modified bentonite;
further, the flavoring agent includes sodium citrate/citric acid, salt, pectin, flavoring essence, sweetener and dextrin mixed in any ratio.
Further, the preparation method of the spirulina oligopeptide comprises the following steps: (1) Adding purified water into spirulina powder, stirring, and decocting to obtain decoction mixture;
(2) Cooling the decoction mixture to 58 ℃, adding cellulase, amylase and saccharifying enzyme, and stirring for enzymolysis to obtain a first enzymolysis liquid;
(3) Centrifuging and separating the first enzymolysis liquid to obtain desugared spirulina powder, adding purified water into the desugared spirulina powder, heating to 58 ℃, adding exo-aspergillus oryzae neutral protease and alkaline protease, and stirring for enzymolysis for 4 hours to obtain a second enzymolysis liquid; wherein the addition of the exo-aspergillus oryzae neutral protease is 0.8% of the weight of the raw material, and the addition of the alkaline protease is 0.3% of the weight of the raw material;
(4) And heating the second enzymolysis liquid to raise the temperature for enzyme inactivation, and separating and purifying to obtain the spirulina oligopeptide.
The invention also discloses a preparation method of the small molecule oligopeptide powder composition, which comprises the following steps:
(a) Selecting components with granularity of 70 meshes or 150 meshes, and respectively weighing the components according to the proportion of the components for later use;
(b) Uniformly mixing bovine collagen oligopeptide, spirulina oligopeptide, pea protein peptide powder and flavoring agent to obtain prepared powder;
(c) Uniformly mixing the Chinese yam, the hawthorn and the angelica dahurica to obtain medicinal and edible additive powder; uniformly mixing apple powder, strawberry powder, lemon powder, spinach powder and carrot powder to obtain fruit and vegetable powder;
(d) Adding the medicinal and edible additive powder and the fruit and vegetable powder obtained in the step (c) into the prepared powder in the step (b), and uniformly mixing to obtain the micromolecular oligopeptide powder composition.
Examples
A small molecular oligopeptide powder composition comprises 60% of bovine collagen oligopeptide powder, 20% of spirulina oligopeptide, 5% of pea protein peptide powder, 2% of flavoring agent, 3% of medicinal and edible additive powder and 8% of fruit and vegetable powder.
Further, the preparation method of the bovine collagen oligopeptide powder comprises the following steps:
(1) And (3) raw material treatment: removing fat, residual meat and the like on the surface of fresh beef bones, crushing the beef bones to 2-3 cm of bone blocks by using a bone crusher, washing the crushed beef bones with clear water until the pH value is neutral, putting the beef bones into a cooking pot, and adding water for cooking to obtain cooking liquid;
(2) Degreasing: immersing the cooking liquid into ethyl acetate for soaking and degreasing, standing, filtering and separating to obtain degreased beef bone liquid; adding defluorinated composite particles, and filtering and separating to obtain defluorinated suspension;
(3) Enzymolysis: adding complex enzyme into the suspension, adding citric acid to adjust the pH value to 5, and carrying out enzymolysis for 2 hours in a constant temperature environment at 45 ℃ to obtain beef bone enzymolysis liquid; the complex enzyme consists of 35wt% of papain, 30% of alkaline protease, 30wt% of trypsin, 17wt% of flavourzyme and 3% of enzyme activator;
(4) Sequentially performing ultrafiltration and gel column chromatography purification to obtain the bovine bone small molecular peptide.
Further, in the step (2), the preparation method of the defluorinated composite particles comprises the following steps:
adding carboxymethyl chitosan into water to prepare carboxymethyl chitosan water solution with the concentration of 1-5 wt%; adding bentonite into carboxymethyl chitosan water solution, ultrasonically stirring for 4-8h, filtering to separate out bentonite, adding bentonite into sodium trimetaphosphate water solution, soaking for 1-3h, filtering to separate out bentonite, and drying to obtain modified bentonite; adding the modified bentonite into ethanol solution of lanthanum nitrate, stirring and reacting for 2-5h, filtering, washing and drying to obtain the modified bentonite;
further, the flavoring agent includes sodium citrate/citric acid, salt, pectin, flavoring essence, sweetener and dextrin mixed in any ratio.
Further, the preparation method of the spirulina oligopeptide comprises the following steps: (1) Adding purified water into spirulina powder, stirring, and decocting to obtain decoction mixture;
(2) Cooling the decoction mixture to 58 ℃, adding cellulase, amylase and saccharifying enzyme, and stirring for enzymolysis to obtain a first enzymolysis liquid;
(3) Centrifuging and separating the first enzymolysis liquid to obtain desugared spirulina powder, adding purified water into the desugared spirulina powder, heating to 58 ℃, adding exo-aspergillus oryzae neutral protease and alkaline protease, and stirring for enzymolysis for 4 hours to obtain a second enzymolysis liquid; wherein the addition of the exo-aspergillus oryzae neutral protease is 0.8% of the weight of the raw material, and the addition of the alkaline protease is 0.3% of the weight of the raw material;
(4) And heating the second enzymolysis liquid to raise the temperature for enzyme inactivation, and separating and purifying to obtain the spirulina oligopeptide.
The invention also discloses a preparation method of the small molecule oligopeptide powder composition, which comprises the following steps:
(a) Selecting components with granularity of 70 meshes or 150 meshes, and respectively weighing the components according to the proportion of the components for later use;
(b) Uniformly mixing bovine collagen oligopeptide, spirulina oligopeptide, pea protein peptide powder and flavoring agent to obtain prepared powder;
(c) Uniformly mixing the Chinese yam, the hawthorn and the angelica dahurica to obtain medicinal and edible additive powder; uniformly mixing apple powder, strawberry powder, lemon powder, spinach powder and carrot powder to obtain fruit and vegetable powder;
(d) Adding the medicinal and edible additive powder and the fruit and vegetable powder obtained in the step (c) into the prepared powder in the step (b), and uniformly mixing to obtain the micromolecular oligopeptide powder composition.
Examples
A small molecular oligopeptide powder composition comprises 70% of bovine collagen oligopeptide powder, 20% of spirulina oligopeptide, 5% of pea protein peptide powder, 2% of flavoring agent, 1% of medicinal and edible additive powder and 1% of fruit and vegetable powder.
Further, the preparation method of the bovine collagen oligopeptide powder comprises the following steps:
(1) And (3) raw material treatment: removing fat, residual meat and the like on the surface of fresh beef bones, crushing the beef bones to 2-3 cm of bone blocks by using a bone crusher, washing the crushed beef bones with clear water until the pH value is neutral, putting the beef bones into a cooking pot, and adding water for cooking to obtain cooking liquid;
(2) Degreasing: immersing the cooking liquid into ethyl acetate for soaking and degreasing, standing, filtering and separating to obtain degreased beef bone liquid; adding defluorinated composite particles, and filtering and separating to obtain defluorinated suspension;
(3) Enzymolysis: adding complex enzyme into the suspension, adding citric acid to adjust the pH value to 7.5, and carrying out enzymolysis for 2 hours in a constant temperature environment at 45 ℃ to obtain beef bone enzymolysis liquid; the complex enzyme consists of 35wt% of papain, 30% of alkaline protease, 30wt% of trypsin, 17wt% of flavourzyme and 3% of enzyme activator;
(4) Sequentially performing ultrafiltration and gel column chromatography purification to obtain the bovine bone small molecular peptide.
Further, in the step (2), the preparation method of the defluorinated composite particles comprises the following steps:
adding carboxymethyl chitosan into water to prepare carboxymethyl chitosan water solution with the concentration of 1-5 wt%; adding bentonite into carboxymethyl chitosan water solution, ultrasonically stirring for 8 hours, filtering to separate out bentonite, adding bentonite into sodium trimetaphosphate water solution, soaking for 3 hours, filtering to separate out bentonite, and drying to obtain modified bentonite; adding the modified bentonite into ethanol solution of lanthanum nitrate, stirring and reacting for 2-5h, filtering, washing and drying to obtain the modified bentonite;
further, the flavoring agent includes sodium citrate/citric acid, salt, pectin, flavoring essence, sweetener and dextrin mixed in any ratio.
Further, the preparation method of the spirulina oligopeptide comprises the following steps: (1) Adding purified water into spirulina powder, stirring, and decocting to obtain decoction mixture;
(2) Cooling the decoction mixture to 58-58 ℃, adding cellulase, amylase and saccharifying enzyme, stirring and performing enzymolysis to obtain a first enzymolysis liquid;
(3) Centrifuging and separating the first enzymolysis liquid to obtain desugared spirulina powder, adding purified water into the desugared spirulina powder, heating to 58 ℃, adding exo-aspergillus oryzae neutral protease and alkaline protease, and stirring for enzymolysis for 4 hours to obtain a second enzymolysis liquid; wherein the addition of the exo-aspergillus oryzae neutral protease is 0.8% of the weight of the raw material, and the addition of the alkaline protease is-0.3% of the weight of the raw material;
(4) And heating the second enzymolysis liquid to raise the temperature for enzyme inactivation, and separating and purifying to obtain the spirulina oligopeptide.
The invention also discloses a preparation method of the small molecule oligopeptide powder composition, which comprises the following steps:
(a) Selecting components with granularity of 70 meshes or 150 meshes, and respectively weighing the components according to the proportion of the components for later use;
(b) Uniformly mixing bovine collagen oligopeptide, spirulina oligopeptide, pea protein peptide powder and flavoring agent to obtain prepared powder;
(c) Uniformly mixing the Chinese yam, the hawthorn and the angelica dahurica to obtain medicinal and edible additive powder; uniformly mixing apple powder, strawberry powder, lemon powder, spinach powder and carrot powder to obtain fruit and vegetable powder;
(d) Adding the medicinal and edible additive powder and the fruit and vegetable powder obtained in the step (c) into the prepared powder in the step (b), and uniformly mixing to obtain the micromolecular oligopeptide powder composition.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (7)
1. The small molecular oligopeptide powder composition is characterized by comprising 58-72% of bovine collagen oligopeptide powder, 20-33% of spirulina oligopeptide, 15-30% of pea protein peptide powder, 2-5% of flavoring agent, 1-3% of medicine and food homologous additive powder and 1-5% of fruit and vegetable powder.
2. The small molecule oligopeptide powder composition according to claim 1, wherein the method for preparing bovine collagen oligopeptide powder comprises the following steps:
(1) And (3) raw material treatment: removing fat, residual meat and the like on the surface of fresh beef bones, crushing the beef bones to 2-3 cm of bone blocks by using a bone crusher, washing the crushed beef bones with clear water until the pH value is neutral, putting the beef bones into a cooking pot, and adding water for cooking to obtain cooking liquid;
(2) Degreasing: immersing the cooking liquid into ethyl acetate for soaking and degreasing, standing, filtering and separating to obtain degreased beef bone liquid; adding defluorinated composite particles, and filtering and separating to obtain defluorinated suspension;
(3) Enzymolysis: adding complex enzyme into the suspension, adding citric acid to adjust the pH value to 5-7.5, and performing enzymolysis for 1-2h in a constant temperature environment of 35-45 ℃ to obtain beef bone enzymolysis solution; the complex enzyme consists of 35wt% of papain, 30% of alkaline protease, 30wt% of trypsin, 17wt% of flavourzyme and 3% of enzyme activator;
(4) Sequentially performing ultrafiltration and gel column chromatography purification to obtain the bovine bone small molecular peptide.
3. The small molecule oligopeptide powder composition according to claim 2, wherein in step (2), the preparation method of the defluorinated composite particles comprises the following steps:
adding carboxymethyl chitosan into water to prepare carboxymethyl chitosan water solution with the concentration of 1-5 wt%; adding bentonite into carboxymethyl chitosan water solution, ultrasonically stirring for 4-8h, filtering to separate out bentonite, adding bentonite into sodium trimetaphosphate water solution, soaking for 1-3h, filtering to separate out bentonite, and drying to obtain modified bentonite; adding the modified bentonite into ethanol solution of lanthanum nitrate, stirring and reacting for 2-5h, filtering, washing and drying to obtain the modified bentonite.
4. The small molecule oligopeptide powder composition according to claim 1, wherein the flavoring agent comprises sodium citrate/citric acid, salt, pectin, flavoring essence, sweetener and dextrin in any ratio.
5. The small molecule oligopeptide powder composition according to claim 1, wherein the preparation method of the spirulina oligopeptide comprises the following steps: (1) Adding purified water into spirulina powder, stirring, and decocting to obtain decoction mixture;
(2) Cooling the decoction mixture to 58-58 ℃, adding cellulase, amylase and saccharifying enzyme, stirring and performing enzymolysis to obtain a first enzymolysis liquid;
(3) Centrifuging and separating the first enzymolysis liquid to obtain desugared spirulina powder, adding purified water into the desugared spirulina powder, heating to 50-58 ℃, adding exo-aspergillus oryzae neutral protease and alkaline protease, and stirring for enzymolysis for 3-4 hours to obtain a second enzymolysis liquid; wherein the addition of the exo-aspergillus oryzae neutral protease is 0.5-0.8% of the weight of the raw material, and the addition of the alkaline protease is 0.1-0.3% of the weight of the raw material;
(4) And heating the second enzymolysis liquid to raise the temperature for enzyme inactivation, and separating and purifying to obtain the spirulina oligopeptide.
6. The preparation method of the small molecule oligopeptide powder composition is characterized by comprising the following steps of:
(a) Selecting components with granularity of 70 meshes or 150 meshes, and respectively weighing the components according to the proportion of the components for later use;
(b) Uniformly mixing bovine collagen oligopeptide, spirulina oligopeptide, pea protein peptide powder and flavoring agent to obtain prepared powder;
(c) Uniformly mixing the Chinese yam, the hawthorn and the angelica dahurica to obtain medicinal and edible additive powder; uniformly mixing apple powder, strawberry powder, lemon powder, spinach powder and carrot powder to obtain fruit and vegetable powder;
(d) Adding the medicinal and edible additive powder and the fruit and vegetable powder obtained in the step (c) into the prepared powder in the step (b), and uniformly mixing to obtain the micromolecular oligopeptide powder composition.
7. Use of the small molecule oligopeptide powder composition of claim 1 in the preparation of a product for enhancing immunity in humans.
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