CN109943611B - Preparation method and application of three-seed peptide and three-way oil - Google Patents
Preparation method and application of three-seed peptide and three-way oil Download PDFInfo
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Abstract
The invention relates to a preparation method of three-seed peptide and three-way oil and application thereof, the method adopts perilla seeds, safflower seeds and evening primrose seeds as raw materials to be mixed, the raw materials are cold-pressed at low temperature, crude oil and dregs are separated, then the dregs are respectively prepared into three-seed peptide powder, and the crude oil is prepared into the three-way oil. The three-seed peptide and the three-way oil prepared by the method are used as raw materials of biological medicine preparations or functional health-care food. The invention takes perilla seeds, safflower seeds and evening primrose seeds as raw materials, mixed meal after oil is extracted by pressing, poultry whole eggs, fish shrimp peptide and food nutrition enhancer are processed by a method of combining microbial fermentation and enzymolysis technology, modern preparation technology and membrane separation technology, and the invention has the characteristics of biological activity and water solubility, and has the effects of oxidation resistance, aging resistance, tumor resistance and organism immunity enhancement. The squeezed crude oil is refined into "three-way" oil, which has the effects of resisting oxidation, aging and tumor, relaxing the bowels, dredging collaterals, and promoting blood circulation.
Description
Technical Field
The invention relates to the field of biological products, in particular to a preparation method and application of three-seed peptide and three-way oil.
Background
Currently, many animal and plant meals or powders contain high protein, generally between 30-50%, and most are used as feed or fertilizer. For example, 20-30% of the protein of the sophora flower bud meal, 40-50% of the protein of the sesame meal, 12% of nitrogen phosphorus potassium, 35-75% of the protein of the peel powder, 6-13% of the nitrogen phosphorus potassium, 40-60% of the protein of the silkworm pupa residue, 40-50% of the protein of the cottonseed meal, 40-50% of the protein of the penicillin protein, 40-45% of the protein of the sesame residue, 8% of the nitrogen phosphorus potassium and the like are excellent protein resources and are not fully developed and utilized. If the peptide is converted into the small molecular active peptide by the biotechnology and is used as a biological medicine preparation or a functional health food raw material, the value is improved by tens of times, and the economic benefit and the social benefit are achieved.
Through determination, the perilla seed meal and the safflower seed meal contain more than 40 percent of protein, and are also rich in 18 macro-elements such as oryzanol, flavone, antiallergic polyphenol, VB1VB2, VE, VK, Ca, Mg, P and the like and trace elements such as iron, magnesium, copper, zinc and the like which are necessary for biology. The health-care food contains 18 amino acids, the total amount of the amino acids can reach 39%, the content of the essential amino acids accounts for 46.7%, and the health-care food has the effects of resisting and preventing cancers, improving the immunity of a human body, reducing blood fat, promoting learning and memory, relieving cough and asthma, resisting aging, eliminating free radicals and resisting allergy in clinical research.
At present, most of perilla seeds and safflower seeds are used as traditional Chinese medicinal materials at home and abroad for compatibility in medical treatment, functional components of the perilla seeds are extracted to prepare biological medicines, health-care foods and cosmetics with various functions, perilla seed meal after oil extraction of the perilla seeds is used as a protein feed raw material and an agricultural fertilizer, but the biological medicine preparation and the functional health-care food which take perilla seed peptide as a main functional component are not reported yet. The tripeptides extracted from perilla seeds, safflower seeds and evening primrose seeds have the following technical problems: firstly, although the protein content of the meal after oil extraction is between 30 and 40 percent, the protein hydrolysis is influenced or the meal is complexed with peptide into insoluble salt to precipitate due to the fact that the meal contains higher tannic acid, tannin, plant fiber and oxalic acid, so that the yield of the peptide is reduced; secondly, the meal contains a certain bitter taste, which affects the taste of the peptide; thirdly, the waste water and the waste residue after the hydrolysis of the dregs are treated and utilized.
Disclosure of Invention
The present invention aims to improve and innovate the disadvantages and problems existing in the background art and provide a preparation method of the three-seed peptide and the three-way oil.
The invention also aims to provide application of the three-seed peptide and the three-way oil.
The method of the invention adopts perilla seeds, safflower seeds and evening primrose seeds as raw materials to be mixed, and dregs and crude oil are separated out through low-temperature cold pressing, wherein:
the three-seed peptide powder is prepared from the dregs, and the specific process steps are as follows:
1. cleaning and crushing raw materials: cleaning the dregs with the required total mass of 80%, airing and crushing, and sieving by a 80-mesh sieve for later use; cleaning fish and shrimp with the required total mass of 10% and mincing for later use; the egg white with 10 percent of the required total mass is washed clean and then is smashed and stirred uniformly together with the shell and the egg for standby.
2. Curing: putting the three prepared raw materials into a fermentation tank together, adding purified water 3 times of the total mass of the raw materials, stirring, heating to 100 ℃, maintaining for 30 minutes, and introducing cold water into the interlayer of the fermentation tank for circulating cooling to 35-45 ℃.
3. Inoculating and fermenting: adding lactobacillus and yeast culture solution into the fermentation tank, and fermenting and culturing at constant temperature for 24-48 hr.
4. Inactivation: heating to 100 deg.C for 30 min, and adding 1 times of purified water.
5. Enzymolysis: after the fermentation and inactivation, when the temperature is reduced to about 50 ℃, adding acidic enzymolysis liquid according to 10-20% of the total mass of the raw materials, hydrolyzing for 12-24 inches at the temperature of 30-38 ℃, and simultaneously carrying out ultrasonic vibration in the enzymolysis process.
6. Decocting and sterilizing: hydrolyzing the fermented materials, adding purified water 1 time of the total weight of the raw materials, heating to 100 deg.C, and maintaining for 30-60 min.
7. Adjusting the pH value: adjusting pH to 5.8-6.8 with sodium bicarbonate water.
8. Separation and filtration: coarse filtration is carried out firstly, and then membrane separation is carried out.
9. Concentration: the filtrate was concentrated in a low-temperature vacuum tank until the specific gravity became 1.05.
10. Spray drying: and (4) pumping the concentrated liquid into a spray drying tower for drying to obtain the three-seed peptide powder.
The crude oil is adopted to prepare the three-way oil, and the specific process steps are as follows:
1. preparing materials: adding the crude oil into a neutralization pot, uniformly stirring, and metering; measuring the acid value and calculating the initial temperature of the alkali amount adjusting oil, wherein the initial temperature is about 30 ℃, and the summer alkali temperature is the oil temperature at that time.
2. Adding alkali for neutralization: the concentration of the alkali liquor is generally 12-18 ℃ Baume, and the alkali liquor is required to be added in about 10 minutes when being sprayed; stirring rapidly at 60-70 rpm.
3. And (3) heating: the speed is preferably one degree per minute, when the temperature is raised to 50 ℃, if the oil soap is separated obviously, the temperature is stopped to rise when the soap grains are thickened from fine, and the soapstock can be kept still for precipitation when the oil path is arranged on the soap grains and the soapstock sinks rapidly.
4. Standing and precipitating: the standing and precipitating time is not less than 4 hours.
5. Washing with water: the oil temperature is 85 ℃ when the water is washed, the temperature of the saline water or the clear water is more than 90 ℃, and the concentration of the saline water is 4%; washing for 2-3 times, and finishing washing when the wastewater discharged from the last time of washing is clean and clear.
6. And (3) drying: heating and drying in an open pot, returning oil when the temperature rises to 100 ℃, stopping heating to volatilize the water, stopping heating when the temperature rises to 118-120 ℃, and preserving the heat for 1 hour to volatilize the water as much as possible to obtain the tee oil.
In one embodiment, the perilla seeds comprise perilla seeds and perilla leaves.
In one embodiment, the perilla seed, the safflower seed and the evening primrose seed are used in a ratio of 1:1: 1.
In one embodiment, the lactobacillus and yeast culture are added in an amount of 10% of the fermentation mass during the step of inoculating fermentation.
In one embodiment, the acidic enzymolysis solution is prepared from the following raw materials: adding 100ml of food-grade hydrochloric acid, 100ml of lactic acid, 100ml of acetic acid, 700ml of purified water, 50g of pepsin and 50g of papain into 1000ml of acidic hydrolysate, wherein the activity of the pepsin is 1: 400.
In one embodiment, the three-way oil is prepared into soft capsules of about 0.5-1ml per capsule.
The comprehensive treatment method of the waste water and the waste residues generated in the three-seed peptide powder process comprises the following steps: collecting waste water and waste residue into fermentation tank, adding Aspergillus niger, fermenting for 24-48 hr, heating, drying, pulverizing, and packaging to obtain the feed immunopotentiator.
The three-seed peptide and the three-way oil prepared by the preparation method are used as raw materials of biological medicine preparations or functional health-care food.
The invention has the advantages and beneficial effects that:
the invention takes perilla seeds (including seeds and leaves), safflower seeds and evening primrose seeds as raw materials, mixed meal after oil is extracted by pressing, poultry whole eggs, fish shrimp peptide and food nutrition enhancer are supplemented, and the invention is processed by a method of combining microbial fermentation and enzymolysis technology, modern preparation technology and membrane separation technology, has the characteristics of biological activity and water solubility, is a small molecular peptide fused with animals and plants, has the molecular weight of less than 500 daltons, has the effects of oxidation resistance, aging resistance, tumor resistance and body immunity enhancement, and is widely applied to biological medicine products and health food raw materials. The squeezed crude oil is refined into "three-way" oil, which has the effects of resisting oxidation, aging and tumor, relaxing the bowels, dredging collaterals, and promoting blood circulation.
The three-seed peptide processing raw material of the invention adopts: 80% of three-seed meal (perilla seed, safflower seed and evening primrose seed), 10% of poultry egg (with shell) and 10% of fish and shrimp. The formula mechanism is as follows: the perilla seeds have the effects of sweating, relieving exterior syndrome, dispersing wind cold, nourishing stomach, removing fishy smell and enhancing flavor; by adding eggs, fish and shrimps, the content of high-quality protein can be increased, and the peptide structure can be optimized. The safflower seed has effects of promoting blood circulation, dredging channels, removing blood stasis, relieving pain, resisting cancer, resisting oxidation, and resisting aging. The oenothera odorata contains abundant linolenic acid, and has the effects of reducing blood fat, strengthening tendons and bones and dispelling wind and dampness. The addition of eggs, fish and shrimp can improve the yield of small molecular peptide and enhance the function of peptide synergistically.
The invention also processes the squeezed oil into a three-way oil product, in particular to a soft capsule which has the effects of relaxing the bowels, beautifying the face, nourishing the skin, resisting oxidation and aging, preventing cardiovascular and cerebrovascular diseases, reducing blood fat and lowering blood pressure.
The invention specifically solves the following problems in the prior art:
firstly, high-quality animal protein such as eggs, fishes and shrimps is added to the raw material formula, so that protein sources are increased, and cellulose, tannin and oxalic acid in the three-seed meal are consumed due to the fact that the animal protein raw material contains certain fat. Secondly, by adopting microbial fermentation, a large amount of cellulose is consumed, tannin is converted into mycoprotein, the protein content is increased, and the hydrolysis rate of the protein can be improved. Thirdly, in the enzymolysis and digestion process, the hydrolysis of protein is accelerated by an ultrasonic means, the complete hydrolysis is promoted, and the yield of the three-seed peptide (perilla seed peptide, safflower seed peptide and oenothera odorata seed peptide) and the content of less than 500Da are improved. Fourthly, adding the dicer enzyme to perform positioned debitterizing and astringency removal, and improving the taste of the product. Fifthly, the waste water and the waste residues generated in the processing are collected, fermented and dried to be processed into the feed reinforcing agent, so that the comprehensive utilization of the processing raw materials and the byproducts is realized.
Detailed Description
In order to facilitate an understanding of the invention, several embodiments of the invention are set forth below. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Example 1:
firstly, mixing perilla seed, leaf, safflower seed and evening primrose seed according to the ratio of 1:1:1, adopting low-temperature cold pressing to separate out crude oil and dregs, and then respectively processing.
Secondly, preparing the three-seed peptide powder by adopting the dregs, wherein the production flow of the product is as follows:
cleaning raw materials, crushing, mixing and decocting, cooling, inoculating and fermenting, decocting and sterilizing, cooling with water, adding acid and enzyme, adjusting pH value, decocting and separating, concentrating, spraying and drying, granulating, drying and subpackaging.
The specific process steps are as follows:
1. cleaning and crushing raw materials: and cleaning the dregs with the required total mass of 80%, airing, crushing and sieving by a 80-mesh sieve for later use. Cleaning fish and shrimp 10% of the total mass, and mincing for later use. The egg white with the required total mass of 10 percent is cleaned and then is smashed and stirred together with the shell and the egg.
2. Curing: putting the three prepared raw materials into a fermentation tank together, adding purified water 3 times the total mass of the raw materials, stirring, heating to 100 ℃, maintaining for 30 minutes, and introducing cold water into the interlayer of the fermentation tank to circularly cool to 35-45 ℃.
3. Inoculating and fermenting: adding lactobacillus and yeast culture solution (10% strain culture stock solution by mass) into the fermentation tank, and fermenting and culturing at constant temperature for 24-48 hr.
4. Inactivation: heating to 100 deg.C for 30 min, and adding 1 times of purified water.
5. Enzymolysis: after the fermentation and inactivation, when the temperature is reduced to about 50 ℃, adding acidic enzymolysis liquid according to 10-20% of the total mass of the raw materials, hydrolyzing for 12-24 inches at the temperature of 30-38 ℃, and simultaneously carrying out ultrasonic vibration in the enzymolysis process. The preparation raw materials of the acidic enzymolysis liquid are as follows: 1000ml of acidic hydrolysate, 100ml of food grade hydrochloric acid, 100ml of lactic acid, 100ml of acetic acid, 700ml of purified water, 50g of pepsin (activity 1: 400) and 50g of papain are added.
6. Decocting and sterilizing: hydrolyzing the fermented material, adding purified water 1 time of the original mass, heating to 100 deg.C, and maintaining for 30-60 min.
7. Adjusting the pH value: adjusting pH value to 5.8-6.8 with sodium bicarbonate water (sodium bicarbonate solution).
8. Separation and filtration: coarse filtration is carried out firstly, and then membrane separation is carried out.
9. Concentration: the filtrate was concentrated in a low-temperature vacuum tank until the specific gravity became 1.05.
10. Spray drying: and (4) pumping the concentrated liquid into a spray drying tower for drying to obtain the three-seed peptide powder.
The comprehensive treatment of the waste water and the waste residues generated in the process comprises the following steps: collecting waste water and waste residue into fermentation tank, adding Aspergillus niger, fermenting for 24-48 hr, heating, drying, pulverizing, and packaging to obtain the feed immunopotentiator.
Thirdly, the crude oil is adopted to prepare the three-way oil capsule, and the processing flow of the product is as follows:
crude oil, adding alkali for neutralization, heating, standing for precipitation, purifying oil, washing with water, drying, filtering and filling into soft capsules.
The specific process steps are as follows:
1. preparing materials: adding the crude oil into a neutralization pot, uniformly stirring, and metering. Measuring the acid value and calculating the initial temperature of the alkali amount adjusting oil, wherein the initial temperature is about 30 ℃, and the summer alkali temperature is the oil temperature at that time.
2. Adding alkali for neutralization: the concentration of the alkali liquor is generally 12-18 ℃ Baume, and the alkali liquor is required to be added completely within about 10 minutes when being sprayed. Stirring rapidly at 60-70 rpm.
3. And (3) heating: the speed is preferably one liter per minute. When the temperature is raised to 50 ℃, if the oil soap is separated obviously, the soap grain is cut from thin to thick, the temperature is stopped rising, and when an oil path is arranged on the soap grain, the soapstock sinks rapidly, and then the soap grain can be kept still for precipitation.
4. Standing and precipitating: the standing and precipitating time is not less than 4 hours.
5. Washing with water: the oil temperature is 85 deg.C, the temperature of saline water or clear water is above 90 deg.C, and the concentration of saline water is 4%. The water washing is generally carried out for 2-3 times, and the water washing is finished when the wastewater discharged from the last time of water washing is clean and clear.
6. And (3) drying: heating and drying in an open pot, returning oil when the temperature rises to 100 ℃, stopping heating to volatilize the water, stopping heating when the temperature rises to 118-120 ℃, and preserving the heat for 1 hour to volatilize the water as much as possible.
7. Making into soft capsule with dosage of 0.5-1 ml.
Example 2:
firstly, mixing perilla seed, leaf, safflower seed and evening primrose seed according to the ratio of 1:1:1, adopting low-temperature cold pressing to separate out crude oil and dregs, and then respectively processing.
Secondly, preparing the three-seed peptide powder by adopting the dregs, wherein the production flow of the product is as follows:
cleaning raw materials, crushing, mixing and decocting, cooling, inoculating and fermenting, decocting and sterilizing, cooling with water, adding acid and enzyme, adjusting pH value, decocting and separating, concentrating, spraying and drying, granulating, drying and subpackaging.
The specific process steps are as follows:
1. cleaning and crushing raw materials: and cleaning the dregs with the required total mass of 80%, airing, crushing and sieving by a 80-mesh sieve for later use. Cleaning fish and shrimp 10% of the total mass, and mincing for later use. The egg white with the required total mass of 10 percent is cleaned and then is smashed and stirred together with the shell and the egg.
2. Curing: putting the three prepared raw materials into a fermentation tank together, adding purified water 3 times the total mass of the raw materials, stirring, heating to 100 ℃, maintaining for 30 minutes, and then introducing cold water into the interlayer of the fermentation tank for circulating cooling to 40 ℃.
3. Inoculating and fermenting: adding lactobacillus and yeast culture solution (10% strain culture stock solution by mass) into the fermentation tank, and fermenting and culturing at constant temperature for 35 hr.
4. Inactivation: heating to 100 deg.C for 30 min, and adding 1 times of purified water.
5. Enzymolysis: after the fermentation and inactivation, when the temperature is reduced to about 50 ℃, acid enzymolysis liquid is added according to 15 percent of the total mass of the raw materials, the raw materials are hydrolyzed for 18 inches at the temperature of 35 ℃, and ultrasonic vibration is carried out in the enzymolysis process. The preparation raw materials of the acidic enzymolysis liquid are as follows: 1000ml of acidic hydrolysate, 100ml of food grade hydrochloric acid, 100ml of lactic acid, 100ml of acetic acid, 700ml of purified water, 50g of pepsin (activity 1: 400) and 50g of papain are added.
6. Decocting and sterilizing: hydrolyzing the fermented material, adding purified water 1 time of the original mass, heating to 100 deg.C, and maintaining for 45 min.
7. Adjusting the pH value: the pH was adjusted to 6.5 with sodium bicarbonate water (sodium bicarbonate solution).
8. Separation and filtration: coarse filtration is carried out firstly, and then membrane separation is carried out.
9. Concentration: the filtrate was concentrated in a low-temperature vacuum tank until the specific gravity became 1.05.
10. Spray drying: and (4) pumping the concentrated liquid into a spray drying tower for drying to obtain the three-seed peptide powder.
The comprehensive treatment of the waste water and the waste residues generated in the process comprises the following steps: and (3) collecting the waste water and the waste residues into a fermentation tank, adding aspergillus niger for fermenting for 35 hours, heating and drying, crushing, packaging and subpackaging to obtain the feed immunopotentiator.
Thirdly, the crude oil is adopted to prepare the three-way oil capsule, and the processing flow of the product is as follows:
crude oil, adding alkali for neutralization, heating, standing for precipitation, purifying oil, washing with water, drying, filtering and filling into soft capsules.
The specific process steps are as follows:
1. preparing materials: adding the crude oil into a neutralization pot, uniformly stirring, and metering. Measuring the acid value and calculating the initial temperature of the alkali amount adjusting oil, wherein the initial temperature is about 30 ℃, and the summer alkali temperature is the oil temperature at that time.
2. Adding alkali for neutralization: the concentration of the alkali liquor is generally 15 baume, and the alkali liquor is required to be added in about 10 minutes when being sprayed. The stirring was carried out rapidly at a speed of 65 rpm.
3. And (3) heating: the speed is preferably one liter per minute. When the temperature is raised to 50 ℃, if the oil soap is separated obviously, the soap grain is cut from thin to thick, the temperature is stopped rising, and when an oil path is arranged on the soap grain, the soapstock sinks rapidly, and then the soap grain can be kept still for precipitation.
4. Standing and precipitating: the standing and precipitating time is not less than 4 hours.
5. Washing with water: the oil temperature is 85 deg.C, the temperature of saline water or clear water is above 90 deg.C, and the concentration of saline water is 4%. The water washing is generally carried out for 2-3 times, and the water washing is finished when the wastewater discharged from the last time of water washing is clean and clear.
6. And (3) drying: heating and drying in an open pot, returning oil when the temperature rises to 100 ℃, stopping heating to volatilize the water, stopping heating when the temperature rises to 118-120 ℃, and preserving the heat for 1 hour to volatilize the water as much as possible.
7. Making into soft capsule with dosage of 0.5-1 ml.
Through years of investigation and research, the invention discovers that the human constitution and the life function are degraded due to the change of the food structure and the quality, the natural environment pollution, the change of the life and working modes and the long-time use of a computer and a mobile phone, so that the immunity of the organism is low, the digestion and metabolism disorder is disordered, large-area and large-scale special variable infectious diseases are frequently outbreaked, and difficult and miscellaneous diseases are even, thereby bringing serious threat to the human health. Therefore, research and development of the tri-seed peptide are very important. The three-seed peptide is prepared by taking three-seed meal protein obtained by mixing perilla seed, safflower seed and evening primrose seed and extracting oil as a main raw material, taking high-quality animal protein such as eggs, fish and shrimps as an auxiliary material, fermenting the three-seed meal protein by using microorganisms, converting protein, carbohydrate, fat, vitamins, macroelements, trace elements and other nutrients in the raw materials into mycoprotein and active nutrients of thallus, and preparing small-molecule active peptide below 500Da and other nutrients or special nutrients chelated with the peptide such as perilla phenol substances, perilla polysaccharide, oligosaccharide (animal polysaccharide) and various trace elements by using an enzymolysis and modern preparation process. The small molecule active peptide has high nutritive value. In the digestive tract, peptides are better and faster in digestive absorption in the gastrointestinal tract than protein and free amino acid, and the peptides are shorter in retention time in the intestines and stomach than protein, have low frequency of gastroptosis and abdominal fullness, reduce the absorption area of the small intestine and damage the digestive function.
In essence, the nutritional effects of peptides are those of proteins and L-amino acids. Wherein the small molecule peptide plays a more positive role than amino acids in the life process, and the protein is synthesized into catabolic process from the body. As a means for supplementing protein, for healthy human bodies, various small-molecule peptides and L-amino acids are mainly obtained from dietary protein, and hormones, enzymes and proteins are synthesized in vivo.
The human body is in a disease state, especially for critically ill patients with serious digestive disorder, after operation and the like. The absorption of food proteins is severely limited and affected and polypeptides cannot be synthesized in vivo. Protein and amino acid circulation is destroyed, so that the supplement of nutrition by common diet is difficult or impossible, and the direct taking of the protein peptide oral liquid for protein supplement is a quick and effective method. The polysaccharide includes plant polysaccharide and animal polysaccharide, and is effective component for improving immunity, resisting and preventing cancer, and promoting metabolism. For example, chitosan oligosaccharide has strong effects of resisting oxidation, resisting tumor, resisting aging, scavenging free radicals in organism, enhancing immunity, etc. The product contains multiple trace elements, vitamins, proteins, hormones, and enzymes. The Chinese medicinal composition organically combines the functional components, and has the effects of resisting oxidation, tumors and aging, removing free radicals in the body, enhancing the immunity of the machine and the like.
The content of the functional components of the three-seed peptide product is as follows:
| component (A) | Protein g | Peptide g | Calcium mg | Zinc mg | Iron mg | Selenium ug | Retinoic acid g | Polysaccharide g |
| Content (wt.) | 75.64 | 74.96 | 15.53 | 23.72 | 634.7 | 930.5 | 1.78 | 9.36 |
The three-way oil product of the invention is a perilla oil soft capsule, and has the effects of relaxing bowels, beautifying, nourishing skin, resisting oxidation and aging, preventing cardiovascular and cerebrovascular diseases, reducing blood fat and lowering blood pressure.
The embodiments of the present invention are described only for the preferred embodiments of the present invention, and not for the limitation of the concept and scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the design concept of the present invention shall fall into the protection scope of the present invention, and the technical content of the present invention which is claimed is fully set forth in the claims.
Claims (5)
1. A preparation method of three-seed peptide and three-way oil is characterized in that the method adopts perilla seeds, safflower seeds and evening primrose seeds in a ratio of 1:1:1 as raw materials to be mixed, and dregs and crude oil are separated through low-temperature cold pressing, wherein:
the three-seed peptide powder is prepared from the dregs, and the specific process steps are as follows:
1) cleaning and crushing raw materials: cleaning the dregs with the required total mass of 80%, airing and crushing, and sieving by a 80-mesh sieve for later use; cleaning fish and shrimp with the required total mass of 10% and mincing for later use; taking the egg white accounting for 10 percent of the total mass, washing the egg white, and then mashing and stirring the egg white together with the shell for standby;
2) curing: putting the three prepared raw materials into a fermentation tank together, adding purified water 3 times the total mass of the raw materials, stirring, heating to 100 ℃, maintaining for 30 minutes, and introducing cold water into the interlayer of the fermentation tank for circulating cooling to 35-45 ℃;
3) inoculating and fermenting: adding lactobacillus and yeast culture solution into the fermentation tank according to 10% of the fermentation material, and fermenting and culturing for 24-48 hours at constant temperature;
4) inactivation: heating to 100 deg.C, maintaining for 30 min, and adding 1 times of purified water;
5) enzymolysis: after the fermentation inactivation, when the temperature is reduced to 50 ℃, adding acidic enzymolysis liquid which is 10-20% of the total mass of the raw materials, hydrolyzing for 12-24 inches at the temperature of 30-38 ℃, and simultaneously carrying out ultrasonic vibration in the enzymolysis process; the preparation raw materials of the acidic enzymolysis liquid are as follows: adding 100ml of food-grade hydrochloric acid, 100ml of lactic acid, 100ml of acetic acid, 700ml of purified water, 50g of pepsin and 50g of papain into 1000ml of acidic hydrolysate, wherein the activity of the pepsin is 1: 400;
6) decocting and sterilizing: hydrolyzing the fermented materials, adding purified water 1 time of the total weight of the raw materials, heating to 100 deg.C, and maintaining for 30-60 min;
7) adjusting the pH value: adjusting pH to 5.8-6.8 with sodium bicarbonate water;
8) separation and filtration: coarse filtration and membrane separation;
9) concentration: concentrating the filtrate in a low-temperature vacuum tank until the specific gravity is 1.05;
10) spray drying: pumping the concentrated liquid into a spray drying tower for drying to obtain the three-seed peptide powder;
the crude oil is adopted to prepare the three-way oil, and the specific process steps are as follows:
1) preparing materials: adding the crude oil into a neutralization pot, uniformly stirring, and metering; measuring the acid value and calculating the alkali amount to adjust the initial temperature of the oil to be 30 ℃;
2) adding alkali for neutralization: the concentration of the alkali liquor is 12-18 ℃ Baume, and the alkali liquor is required to be added in 10 minutes when being sprayed; stirring rapidly at 60-70 r/min;
3) and (3) heating: when the speed is increased to 50 ℃ at one liter per minute, if the oil soap is separated obviously, the soap grain is changed from thin to thick, the temperature is stopped rising, and when an oil way is arranged on the soap grain, the soapstock sinks rapidly, the soap grain can be kept still for precipitation;
4) standing and precipitating: standing for precipitation time not less than 4 hours;
5) washing with water: the oil temperature is 85 ℃ when the water is washed, the temperature of the saline water or the clear water is more than 90 ℃, and the concentration of the saline water is 4%; washing for 2-3 times, and finishing washing when the wastewater discharged from the last time of washing is clean and clear;
6) and (3) drying: heating and drying in an open pot, returning oil when the temperature rises to 100 ℃, stopping heating to volatilize the water, stopping heating when the temperature rises to 118-120 ℃, and preserving the heat for 1 hour to obtain the tee oil.
2. The method according to claim 1, wherein the perilla seeds comprise perilla seeds and perilla leaves.
3. The method of claim 1, wherein the triplet oil is formulated into 0.5-1ml soft capsules per capsule.
4. The preparation method according to claim 1 or 2, characterized in that the comprehensive treatment method of wastewater and waste residues generated in the three-seed peptide powder process comprises the following steps: collecting waste water and waste residue into fermentation tank, adding Aspergillus niger, fermenting for 24-48 hr, heating, drying, pulverizing, and packaging to obtain the feed immunopotentiator.
5. Use of the tri-seed peptide and the tri-oil obtained by the preparation method according to any one of claims 1 to 3, wherein the tri-seed peptide and the tri-oil are used as raw materials of functional health foods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910135532.9A CN109943611B (en) | 2019-02-25 | 2019-02-25 | Preparation method and application of three-seed peptide and three-way oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910135532.9A CN109943611B (en) | 2019-02-25 | 2019-02-25 | Preparation method and application of three-seed peptide and three-way oil |
Publications (2)
| Publication Number | Publication Date |
|---|---|
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| CN109943611B true CN109943611B (en) | 2021-03-30 |
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| WO2007148889A1 (en) * | 2006-06-22 | 2007-12-27 | E.S.Biotech. Co., Ltd. | Edible plant oils from which saturated fatty acids were removed and manufacturing process thereof |
| CN104004583A (en) * | 2014-06-06 | 2014-08-27 | 云南省农业科学院高山经济植物研究所 | Natural health plant oil containing linoleic acid and linolenic acid as well as preparation method and application of plant oil |
| CN106675778A (en) * | 2017-03-06 | 2017-05-17 | 贵州侗乡生态农业科技发展有限公司 | Perilla oil refining method |
| CN109112174A (en) * | 2018-10-26 | 2019-01-01 | 吉林省全民健食品有限公司 | A kind of oenothera seed peptide and its preparation method and application |
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| WO2007148889A1 (en) * | 2006-06-22 | 2007-12-27 | E.S.Biotech. Co., Ltd. | Edible plant oils from which saturated fatty acids were removed and manufacturing process thereof |
| CN104004583A (en) * | 2014-06-06 | 2014-08-27 | 云南省农业科学院高山经济植物研究所 | Natural health plant oil containing linoleic acid and linolenic acid as well as preparation method and application of plant oil |
| CN106675778A (en) * | 2017-03-06 | 2017-05-17 | 贵州侗乡生态农业科技发展有限公司 | Perilla oil refining method |
| CN109112174A (en) * | 2018-10-26 | 2019-01-01 | 吉林省全民健食品有限公司 | A kind of oenothera seed peptide and its preparation method and application |
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| 茶油的精炼;柳州市油脂厂;《中国油脂》;19821231(第2期);63-64 * |
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