CN101696444A - Polypeptide extract as well as preparation method and application thereof - Google Patents
Polypeptide extract as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a polypeptide extract which is mainly prepared from animal by-products, such as the skin and blood of pigs, cows, sheep or fishes by microbial fermentation and enzymolysis of biological enzyme. The preparation method comprises the steps of pregelatinization, microbial fermentation, primary digestion, primary separation and purification, secondary digestion, secondary separation and purification and the like. The polypeptide extract has favorable solubility, high yield, high quality and obvious health-care efficacy. The preparation method of the polypeptide extract has simple preparation process, high resource utilization rate and low cost. The polypeptide extract can be effectively applied to the preparation of hematinic health products.
Description
Technical field
The present invention relates to a kind of nutritional additive and its production and application, relate in particular to peptide species and its production and application.
Background technology
Population of China has 70% to be in sub-health state, and 15% crowd is in morbid state, has only 15% to be healthy population.Along with the aging of China's population, the elderly has accounted for 13% of population.Through for many years investigation, discover that the elderly is along with the increase at age, progressively in decline, a large amount of losses of nutritive element then cause the elderly weak and sickly to each function of health, hypoimmunity.Polypeptide have promote to grow, enhance metabolism, effect such as enhance immunity power.Yet most in the market polypeptide products content is lower, of low quality, is difficult to satisfy human body (especially the elderly's) needs.
At present, the preparation of biological activity peptide product adopts proteolytic enzyme to carry out enzyme digestion reaction under optimum temperuture and pH value condition mainly by enzymolysis process.This method is carried out under comparatively gentle condition, can control production preferably, and preserves amino acid whose nutritive value.Though enzyme hydrolysis method is a kind of method preferably, its production process technology complexity, the purification difficulty is big, pH value, temperature etc. are required strictness, and the solubility of product is little, yield rate is low, and large-scale production is had certain restriction.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, provide that a kind of solubility is good, yield rate is high, the polypeptide extract of taking from animal byproducts of good product quality, obvious health-care efficacy, provide also that a kind of technology is simple, resource utilization is high, cost is little, the preparation method of the polypeptide extract of good product quality, also provide a kind of polypeptide extract of the present invention in the application in healthcare products are enriched blood in preparation.
For solving the problems of the technologies described above, the technical scheme that the present invention proposes is a kind of polypeptide extract, and it is characterized in that: described polypeptide extract is mainly prepared behind microbial fermentation, biological enzyme enzymolysis by animal byproducts.
In the above-mentioned polypeptide extract, described animal byproducts preferably includes skin and the blood of animal, and described animal preferably includes pig, ox, sheep or fish.
In the above-mentioned polypeptide extract, described microorganism preferably includes Saccharomycodes (Saccharomyces), bacillus (Bacillus Cohn) and aspergillus niger and belongs to (Aspergillus niger); Described biological enzyme preferably includes stomach en-and trypsinase.
Under total technical conceive, the present invention also provides a kind of preparation method of above-mentioned polypeptide extract, may further comprise the steps:
(1) pre-gelatinization: animal skin (preferably including pigskin, ox-hide, sheepskin or the fish-skin) chopping with 1 mass parts, add Digestive system again and make pH value≤2, mixture is decocted make its gelatinization; Described Digestive system mainly is made up of pure water and the acid of mixed edible level; The acid of described mixed edible level is preferably the mixture that edible hydrochloric acid (concentration 10%～20%), lactic acid (concentration 90%～95%) and food grade acetic acid are formed;
(2) microbial fermentation: the mashed prod after the above-mentioned pre-gelatinization is mixed with the animal blood (preferably including pig blood, ox blood, sheep blood or fish blood) of 1～2 mass parts, carry out combined fermentation to wherein adding Saccharomycodes, bacillus and aspergillus niger genus;
(3) once digestion: add described Digestive system (preferably making pH value≤1) in the mixture after above-mentioned fermentation, add stomach en-simultaneously, add water again and stir, digest keeping to leave standstill once under the pepsin activity condition;
(4) flash liberation is purified: add ionized water in above-mentioned once postdigestive mixture, carry out separating, removing slag after the boiling again, obtain crude extract;
(5) secondary digestion: get above-mentioned crude extract adjust pH to 6～7, cooling back adds trypsinase, carries out secondary digestion keeping to leave standstill under the tryptic activity condition;
(6) secondary separation is purified: the mixed solution behind the above-mentioned secondary digestion is boiled, is cooled to 1 ℃～5 ℃ then, remove upper strata suspended substance and bottom throw out behind the standing demix, in the middle of getting clear liquor filter extracting solution; With described extracting solution concentrate, after the drying the finished product polypeptide extract.
In above-mentioned preparation method's pre-gelatinization step: the addition of described pure water is preferably 2～5 times of described animal skin quality; Pressure preferably is controlled at 0.2KPa～0.25KPa during described decoction, and the temperature during decoction preferably is controlled at 120 ℃～140 ℃, and decocting time preferably is controlled at 2h～5h.
In above-mentioned preparation method's microbial fermentation step: the total addition level that described Saccharomycodes, bacillus and aspergillus niger belong to three kinds of microorganisms is preferably 1%～5% of described animal byproducts total mass; The mass ratio that described Saccharomycodes, bacillus and aspergillus niger belong to is preferably 1: (1～2): (1～2), the time of described combined fermentation is preferably 48h～72h.
In above-mentioned preparation method's a digestion step: the addition of described Digestive system is preferably 40%～65% of described animal byproducts total mass; Described pepsic addition is preferably 0.5%～2% of described animal byproducts total mass; Described temperature when once digesting preferably is controlled at 20 ℃～45 ℃, and once Xiao Hua time is preferably 24h～48h.
In above-mentioned preparation method's flash liberation purification step: the addition of described ionized water is preferably once 3～5 times of digestion back mixture quality; Pressure during described boiling preferably is controlled at 0.2KPa～0.3KPa, and the temperature during boiling preferably is controlled at 120 ℃～150 ℃, and cooking time is preferably 4h～6h.
In above-mentioned preparation method's secondary digestion step: the reagent of described adjust pH is preferably calcium oxide; Temperature during described secondary digestion preferably is controlled at 40 ℃～50 ℃, and the time of secondary digestion is preferably 5h～8h.
Under total technical conceive, the present invention also provides a kind of above-mentioned application of polypeptide extract in healthcare products are enriched blood in preparation.Polypeptide itself have promote to grow, enhance metabolism, effect such as enhance immunity power, and animal skin the skin furan of human body is had preferably delay senility, the crease-resistant effect that removes line, animal blood not only can enrich blood, but also have the effect of a surname's reason lung clearly.Polypeptide extract of the present invention and different Chinese medicinal materials compatibilities can be made the healthcare product of different efficacies.
Preferably, get the component of following mass parts:
10～35 parts of polypeptide extracts of the present invention
30～50 parts of animal skeletons
5～20 parts of matrimony vines
5～20 parts of the Radixs Astragali
5～20 parts of Radix Codonopsis
5～20 parts of Radix Angelicae Sinensis
5～15 parts of Chinese yams and
The vitamins D trace;
But above-mentioned matrimony vine tonifying kidney for improving eyesight, but Radix Astragali invigorating qi and refreshing, Radix Angelicae Sinensis is the medicinal material of enriching blood preferably, Radix Codonopsis and Chinese yam then have the spleen benefiting and stimulating the appetite qi invigorating functions, vitamins D is treatment rickets and osteoporotic good medicine, can promote the absorption of calcium, with described animal skeleton, matrimony vine, the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, Chinese yam decocts and makes Chinese medicine extract, in this Chinese medicine extract, add described polypeptide extract and vitamins D again, can make the healthcare products with nourishing blood function that contain polypeptide extract of the present invention, these healthcare products also have the osteoporosis of improvement simultaneously, improve the effects such as immunizing power of body.
Compared with prior art, the invention has the advantages that: at first, the present invention has made full use of comparatively cheap animal byproducts and has extracted the polypeptide products that has obtained high added value, high nutritive value, utilize polypeptide extract of the present invention and existing medicinal material component to carry out compatibility, can develop and have different efficacies (for example blood yiqi, enhance metabolism, enhance immunity power etc.) various healthcare products, have good economic benefit and social benefit; Secondly, the present invention has filtered out effective microbial strains and combination by a large amount of experimental analyses, make multiple microorganism synergy during the fermentation be effectively played, fermenting process fully, has fully improved the solubility and the yield rate of biologically active polypeptides greatly; Once more, metabolism by microorganism of the present invention, amino acid and little peptide moved connect, reset and secrete, some peptide bitter taste group is modified and recombinated, can effectively remove the bioactive peptide bitter taste, effectively remove the heavy metal element in the product, and give polypeptide some bioactive functions, the quality of product is further promoted.In addition, the present invention is by the metabolism and the fermentation condition of preferred controlling microbial, can produce the different aminoacids ordering peptide different with molecular weight, during the fermentation, the total free aminoacids that produces is absorbed once more by microorganism, metabolism to microorganism can not produce feedback inhibition, has improved the efficient of preparation feedback greatly.Preparation method of the present invention is owing to made full use of animal byproducts, so the technology cost is little, resource utilization is high, has favorable economic benefit.
Embodiment
Embodiment:
A kind of polypeptide extract of the present invention, it mainly, is prepared behind stomach en-and trypsin digestion through yeast, genus bacillus and fermentation of Aspergillus niger by the by product (pigskin and pig blood) of pig.
The concrete preparation method of aforementioned polypeptides extract may further comprise the steps:
(1) pre-gelatinization: the pigskin of 30g～40g is shredded with machinery, add Digestive system, Digestive system is mainly formed (Digestive system pH value≤1) by the pure water of 80g (2～3 times get final product to the pigskin quality) and an amount of mixed edible level acid, is 1～2 with the pH value of controlling mixture; This mixed edible level acid comprises that concentration is that 10% edible hydrochloric acid, concentration are 95% lactic acid and alimentary acetic acid, and edible hydrochloric acid, lactic acid and the alimentary acetic acid mass concentration in Digestive system is respectively 1%～2%, 1%～2% and 3%～4%; Mixture is decocted 2h～5h make its gelatinization, the pressure-controlling during decoction is at 0.25KPa, and the temperature during decoction is controlled at 140 ℃;
(2) microbial fermentation: the mashed prod after the above-mentioned pre-gelatinization is mixed with the animal blood of 50g～70g, carry out combined fermentation to wherein adding Saccharomycodes, bacillus and aspergillus niger genus, the total addition level that Saccharomycodes, bacillus and aspergillus niger belong to three kinds of microorganisms is 3% of used pigskin and a pig blood total mass; The mass ratio of yeast, genus bacillus and aspergillus niger is 1: 1.5: 1.5 (1: (1～2): all can be effective in the scope of (1～2)), and fermentation time is 60h (all can reach the technique effect of present embodiment in 48h～72h scope);
(3) once digestion: the Digestive system (used Digestive system in the step (1)) that adds used pigskin and pig blood total mass 50% (all can be effective in 40%～65% the scope) in the mixture after above-mentioned fermentation, the stomach en-(1: 400) that adds used pigskin and pig blood total mass 1% (all can reach the technique effect of present embodiment in 0.5%～2% scope) simultaneously, doubling, the water of mixture quality stirs after above-mentioned fermentation again, leave standstill once and digest keeping under the pepsin activity condition (20 ℃～45 ℃), digestion time is 48h (all can reach the technique effect of present embodiment in 24h～48h scope);
(4) flash liberation is purified: add ionized water in above-mentioned once postdigestive mixture, the addition of ionized water is for once digesting 3 times (adding 3～5 times of technique effects that all can reach present embodiment) of back mixture quality, carry out boiling again, pressure-controlling during boiling is at 0.2KPa, temperature during boiling is controlled at 135 ℃, cooking time is 5h, separates after the boiling, removes slag, and obtains crude extract;
(5) secondary digestion: get above-mentioned crude extract with calcium oxide adjust pH to 6～7, treat to add trypsin 2: 10000～4: 10000 after temperature is cooled to 40 ℃～50 ℃), keeping insulation digestion 6h under the tryptic activity condition;
(6) secondary separation is purified: the mixed solution behind the above-mentioned secondary digestion is boiled 1h, put into cooling tower then and be cooled to 1 ℃～5 ℃, remove upper strata suspended substance (mainly comprising lipoid material) and bottom throw out (mainly comprising heavy metal substance) behind the standing demix (leaving standstill the technique effect that 24h～48h all can reach present embodiment), get in the middle of clear liquor filter extracting solution; With described extracting solution by nanometer film concentrate, be spray dried to behind the powdery the finished product polypeptide extract.
Prepare the healthcare products of enriching blood with being used for behind above-mentioned polypeptide extract that makes and the Chinese medicinal materials compatibility, wherein the component of each raw material is as follows:
10～35 parts of the polypeptide extracts that present embodiment makes
30～50 parts of animal skeletons
5～20 parts of matrimony vines
5～20 parts of the Radixs Astragali
5～20 parts of Radix Codonopsis
5～20 parts of Radix Angelicae Sinensis
5～15 parts of Chinese yams and
Vitamins D trace (only adding 40mg in the per 100 tons of products of present embodiment);
Above-mentioned animal skeleton, matrimony vine, the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, Chinese yam decocted make Chinese medicine extract, in this Chinese medicine extract, add described polypeptide extract and vitamins D again, can make the healthcare products with nourishing blood function (capsule formulation) that contain polypeptide extract of the present invention after adding simple processing, packing.
Carry out human feeding trial with above-mentioned healthcare products with nourishing blood function.
Test method: the picked at random age is carried out routine physical examination 26～45 years old women, selection refers to that the women of blood content of hemoglobin＜120g/L carries out blood, urine and the just inspection of project such as routine and liver function, gets rid of and determines that 104 women are as study subject behind other relative diseases.At random these 104 women are divided into control group (51 people) and test-meal group (53 people) by content of hemoglobin, two groups of women's basic condition is as shown in table 1 below, does not have significant difference by the visible two groups of women's of table 1 basic condition.The test-meal group is taken the healthcare products of present embodiment preparation, and control group gives outer packaging identical placebo, and the mode of taking is every day twice, and each two, the observation period is one month.Adopt questioning to carry out dietary survey to two groups of experimenters before on-test and during off-test, two groups of women's that tried macronutrient intake does not have significant difference before and after the test that the survey showed that.
Table 1 is tried anaemia women basic condition relatively
| Group | Experimenter's number | Age (y) | Height (cm) | Body weight (Kg) |
| The test-meal group | ??53 | ??35.8±5.0 | ??158.2±3.7 | ??54.1±5.6 |
| Group | Experimenter's number | Age (y) | Height (cm) | Body weight (Kg) |
| Control group | ??51 | ??37.0±5.1 | ??157.4±3.0 | ??52.7±5.1 |
| The T value | ??1.175 | ??1.208 | ??1.358 | |
| The P value | ??0.242 | ??0.230 | ??0.177 |
After the healthcare products of advance copy embodiment, to experimenter's blood, urine and just the influence of conventional index see the following form 2, by table 2 as seen, test cross-reference group and test-meal group experimenter's red corpuscle and white blood cell count(WBC) all in normal range, routine urinalysis and just also no abnormality seen of routine.
Table 2: to experimenter's blood, urine and the just influence of conventional index (M ± SD)
After the healthcare products of advance copy embodiment, the influence of experimenter's part blood biochemistry index is seen the following form 3, by table 3 as seen, every index content of test cross-reference group and test-meal group experimenter is all in normal range.
Table 3: to the influence of experimenter's part blood biochemistry index (M ± SD)
After the healthcare products of advance copy embodiment, to the influence of experimenter's oxyphorase index 4 (the adopting the high iron processes of conventional cyaniding to measure) of seeing the following form, by table 4 as seen, before the test in control group and the test-meal group experimenter's content of hemoglobin do not have significant difference (T=0.007, P=0.994); Experimenter's content of hemoglobin and increased value are significantly higher than control group (T=9.704, P＜0.001 and T=10.243, P＜0.001) in the test back test-meal group; With the test before compare, during off-test in the test-meal group experimenter's content of hemoglobin than the test before remarkable increase (T=13.733, P＜0.001) is arranged.
Table 4: to the influence of experimenter's content of hemoglobin (g/L)
| Group | Experimenter's number | Before the experiment | After the test | Increased value | The T value | The P value |
| The test-meal group | ??53 | ??109.8±8.0 | ??132.2±12.0 # | ??22.5±11.9 | ??13.733 | ??0.000 |
| Control group | ??51 | ??109.7±8.9 | ??111.8±9.6* | ??2.1±8.4* | ??1.791 | ??0.079 |
| The T value | ??0.007 | ??9.704 | ??10.243 | |||
| The P value | ??0.994 | ??0.000 | ??0.000 |
Annotate: compare * P＜0.001 with the test-meal group, compare before and after self
#P＜0.001.
After the healthcare products of advance copy embodiment, to the influence of experimenter's free erythrocyte protoporphyrin content 5 (the adopting conventional fluorometry to measure) of seeing the following form, by table 5 as seen, before the test in control group and the test-meal group experimenter's free erythrocyte protoporphyrin content do not have significant difference (T=0.984, P=0.327); Experimenter's free erythrocyte protoporphyrin content significantly is lower than control group (T=9.601, P＜0.001) in the test back test-meal group; With the test before compare, during off-test in the test-meal group experimenter's free erythrocyte protoporphyrin content than the test before remarkable reduction (T=7.859, P＜0.001) is arranged.
Table 5: to the influence of experimenter's free erythrocyte protoporphyrin content (μ g/dl)
| Group | Experimenter's number | Before the experiment | After the test | Difference | The T value | The P value |
| The test-meal group | ??53 | ??67.8±13.5 | ??46.0±18.4 ## | ??-21.8±20.2 | ??7.859 | ??0.000 |
| Control group | ??51 | ??70.1±9.9 | ??73.8±10.6 #* | ??3.8±10.9* | ??2.540 | ??0.014 |
| The T value | ??0.984 | ??9.601 | ??8.167 | |||
| The P value | ??0.327 | ??0.000 | ??0.000 |
Annotate: compare * P＜0.001 with the test-meal group, compare before and after self
#P＜0.05,
##P＜0.001.
After the healthcare products of advance copy embodiment, to the influence of experimenter's serum ferritin content 6 (conventional immunization is measured) of seeing the following form, by table 6 as seen, before the test in control group and the test-meal group experimenter's serum ferritin content do not have significant difference (T=0.601, P=0.549); Experimenter's serum ferritin content is significantly higher than control group (T=6.792, P＜0.001) in the test back test-meal group; With the test before compare, be significantly increased before the test of experimenter's serum ferritin content ratio in test-meal group and the control group during off-test (T=13.260, P＜0.001 and T=2.803, P=0.007), but the significance degree of difference is higher than control group (T=7.179, P＜0.001) before and after the test-meal group.
Table 6: to the influence of experimenter's serum ferritin content (μ g/l)
| Group | Experimenter's number | Before the experiment | After the test | Difference | The T value | The P value |
| The test-meal group | ??53 | ??36.9±17.9 | ??66.3±19.6 ## | ??29.3±16.1 | ??13.260 | ??0.000 |
| Control group | ??51 | ??34.9±17.2 | ??41.3±18.3 #* | ??6.4±16.9* | ??2.803 | ??0.007 |
| The T value | ??0.601 | ??6.792 | ??7.179 | |||
| The P value | ??0.549 | ??0.000 | ??0.000 |
Annotate: compare * P＜0.001 with the test-meal group, compare before and after self
#P＜0.01,
##P＜0.001.
By above trial report and testing data as seen, taking the present embodiment healthcare products can make experimenter's content of hemoglobin and serum ferritin content significantly raise after one month, free erythrocyte protoporphyrin content significantly reduces, compare before and after the test-meal group self, oxyphorase amplitude＞the 20g/L that on average raises, and significant significant difference has been compared in test-meal group test back with control group.Therefore, the healthcare products that make of present embodiment have the effect that improves the nutritional anemia function.
Claims (10)
1. polypeptide extract, it is characterized in that: described polypeptide extract is mainly prepared behind microbial fermentation, biological enzyme enzymolysis by animal byproducts.
2. polypeptide extract according to claim 1 is characterized in that: described animal byproducts comprises skin and the blood of animal, and described animal comprises pig, ox, sheep or fish.
3. polypeptide extract according to claim 1 and 2 is characterized in that: described microorganism comprises that Saccharomycodes (Saccharomyces), bacillus (Bacillus Cohn) and aspergillus niger belong to (Aspergillus niger); Described biological enzyme comprises stomach en-and trypsinase.
4. the preparation method of a polypeptide extract as claimed in claim 1 or 2 may further comprise the steps:
(1) pre-gelatinization: the animal skin chopping with 1 mass parts, add Digestive system again and make pH value≤2, mixture is decocted make its gelatinization; Described Digestive system mainly is made up of pure water and the acid of mixed edible level;
(2) microbial fermentation: the mashed prod after the above-mentioned pre-gelatinization is mixed with the animal blood of 1～2 mass parts, carry out combined fermentation to wherein adding Saccharomycodes, bacillus and aspergillus niger genus;
(3) once digestion: add described Digestive system in the mixture after above-mentioned fermentation, add stomach en-simultaneously, add water again and stir, digest keeping to leave standstill once under the pepsin activity condition;
(4) flash liberation is purified: add ionized water in above-mentioned once postdigestive mixture, carry out separating, removing slag after the boiling again, obtain crude extract;
(5) secondary digestion: get above-mentioned crude extract adjust pH to 6～7, cooling back adds trypsinase, carries out secondary digestion keeping to leave standstill under the tryptic activity condition;
(6) secondary separation is purified: the mixed solution behind the above-mentioned secondary digestion is boiled, is cooled to 1 ℃～5 ℃ then, remove upper strata suspended substance and bottom throw out behind the standing demix, in the middle of getting clear liquor filter extracting solution; With described extracting solution concentrate, after the drying the finished product polypeptide extract.
5. the preparation method of polypeptide extract according to claim 4 is characterized in that, in described pre-gelatinization step: the addition of described pure water is 2～5 times of described animal skin quality; Pressure-controlling is at 0.2KPa～0.25KPa during described decoction, and the temperature during decoction is controlled at 120 ℃～140 ℃, and decocting time is controlled at 2h～5h.
6. the preparation method of polypeptide extract according to claim 4, it is characterized in that in described microbial fermentation step: the total addition level that described Saccharomycodes, bacillus and aspergillus niger belong to three kinds of microorganisms is 1%～5% of a described animal byproducts total mass; The mass ratio that described Saccharomycodes, bacillus and aspergillus niger belong to is 1: (1～2): (1～2), the time of described combined fermentation is 48h～72h.
7. the preparation method of polypeptide extract according to claim 4 is characterized in that, in a described digestion step: the addition of described Digestive system is 40%～65% of a described animal byproducts total mass; Described pepsic addition is 0.5%～2% of a described animal byproducts total mass; Described temperature when once digesting is controlled at 20 ℃～45 ℃, and once Xiao Hua time is 24h～48h.
8. the preparation method of polypeptide extract according to claim 4 is characterized in that, in described flash liberation purification step: the addition of described ionized water is once 3～5 times of digestion back mixture quality; Pressure-controlling during described boiling is at 0.2KPa～0.3KPa, and the temperature during boiling is controlled at 120 ℃～150 ℃, and cooking time is 4h～6h.
9. the preparation method of polypeptide extract according to claim 4 is characterized in that, in described secondary digestion step: the reagent of described adjust pH is calcium oxide; Temperature during described secondary digestion is controlled at 40 ℃～50 ℃, and the time of secondary digestion is 5h～8h.
10. the application of polypeptide extract in healthcare products are enriched blood in preparation as claimed in claim 1 or 2 or claim 4 prepares.
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| CN103168983A (en) * | 2011-12-23 | 2013-06-26 | 王士哲 | High-activity multi-nutrition active polypeptide for enhancing immunity |
| CN105219831A (en) * | 2015-11-06 | 2016-01-06 | 潘爱国 | A kind of yak small-molecular peptides of extracting from yak bone and extracting method thereof |
| CN105238831A (en) * | 2015-11-06 | 2016-01-13 | 潘爱国 | Moringa seed micro-molecular peptide extracted from moringa seeds, and extraction method thereof |
| CN105255985A (en) * | 2015-11-06 | 2016-01-20 | 潘爱国 | Crocodile small molecule peptide extracted from bone of crocodile and extraction method of crocodile small molecule peptide |
| CN105368902A (en) * | 2015-11-06 | 2016-03-02 | 潘爱国 | Young pigeon small molecule peptide extracted from young pigeon bone and extraction method thereof |
| CN106755257A (en) * | 2016-12-30 | 2017-05-31 | 潍坊医学院 | A kind of preparation method of earthworm polypeptide extract |
| CN106755256A (en) * | 2016-12-30 | 2017-05-31 | 潍坊医学院 | A kind of preparation method of ground bettle polypeptide, the ground bettle polypeptide for preparing and its application |
| CN108753890A (en) * | 2018-06-20 | 2018-11-06 | 百生康（北京）健康产业科技有限公司 | A kind of extracting method of crocodile whip peptide |
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