CN116987681A - 10-deacetylbaccatin III 10 beta-O-acetyltransferase mutant and application thereof - Google Patents

10-deacetylbaccatin III 10 beta-O-acetyltransferase mutant and application thereof Download PDF

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CN116987681A
CN116987681A CN202210444105.0A CN202210444105A CN116987681A CN 116987681 A CN116987681 A CN 116987681A CN 202210444105 A CN202210444105 A CN 202210444105A CN 116987681 A CN116987681 A CN 116987681A
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朱平
陈天娇
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Institute of Materia Medica of CAMS
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Abstract

The invention belongs to the field of bioengineering, relates to a 10-deacetylbaccatin III 10 beta-O-acetyltransferase mutant and application thereof, and provides a series of 10-deacetylbaccatin III 10 beta-O-acetyltransferase (DBAT) mutant proteins which can specifically transfer acyl groups such as acetyl coenzyme A and the like to a C10 hydroxyl group of 10-deacetyltaxane to generate taxol or analogues thereof. The invention relates to amino acid sequences of DBAT mutants, nucleotide sequences encoding the amino acid sequences, and application of DBAT mutant proteins in synthesizing taxol or analogues thereof by utilizing 10-deacetyltaxane substrates such as 10-deacetyltaxol and the like; in particular, 10-deacetyl taxol is used as a substrate, and acetyl coenzyme A is used as an acyl donor to directly synthesize taxol.

Description

10-deacetylbaccatin III 10 beta-O-acetyltransferase mutant and application thereof
Technical Field
The invention belongs to the field of bioengineering, and in particular relates to a series of mutant proteins of 10-deacetylbaccatin III 10 beta-O-acetyltransferase (DBAT), which transfer acyl groups from but not limited to acetyl coenzyme A to 10-deacetyltaxane to generate taxol or analogues thereof; acyl acceptors for mutant proteins include, but are not limited to, 10-desacetyltaxol and 10-desacetylbaccatin III. The invention relates to amino acid sequences of these mutant proteins, nucleotide sequences encoding these amino acid sequences and uses thereof.
Background
10-De-acetyltransferase (DBAT) is an essential acylase in the taxol biosynthetic pathway and is responsible for catalyzing the acetylation of the hydroxyl group at the C-10 position on 10-De-acetylbaccatin III (10-DAB), the product baccatin III being the last diterpene intermediate in the taxol biosynthetic pathway [ Croteau R, et al, taxol biosynthesis and molecular genetics, phytochem Rev.,2006,5 (1): 75-97].
DBAT also has acetylation of the unnatural substrate 10-Deacetyltaxol (DT) [ Li BJ, et al, improving 10-deacetylbaccatin III-10-beta-O-acetyltransferase catalytic fitness for Taxol production Nat Commun.,2017,8:15544-15556.]Paclitaxel can be produced by only one step of acetylation reaction on the hydroxyl group at the C-10 position,). However, the acetylation efficiency of DT by wild-type DBAT (exemplified by DBAT derived from Taxus northeast) is extremely low (0.26U/mg), about 0.13% of that of its catalytic natural substrate 10-DAB (206.6U/mg).
The applicant of the present invention has performed protein engineering on DBATs of 6 different yew sources, and known that the DBATs of the 6 different sources have higher sequence consistency (about 98%), and only 21 different sites from each other (fig. 1), but the 6 DBATs have larger difference on the catalytic efficiency of DT, wherein the activity of DBATs to acetylate DT is the highest [ Li BJ, et al, improving 10-deacetylbaccatin iii-10- β -O-acetyltransferase catalytic fitness for Taxol production. The DBATcus three-dimensional structure obtained by homologous modeling is analyzed, and the difference sites are far away from the catalytic center, and the rule of affecting the catalytic efficiency is not known, so that the applicant of the invention performs DNA buffering on the 6 DBATs, and the random combination of different difference sites can be realized by breaking and rearranging template sequences of different sources. Through a large number of screening, a series of mutants containing R345C mutation are obtained, the activity of the acetylated DT is obviously improved, and the activity can reach more than 11 times of DBAT (DBATcus) from Taxus northeast.
Disclosure of Invention
The technical problems to be solved are as follows: the invention provides a DBAT series mutant protein containing R345C mutation, a nucleotide sequence for encoding the mutant protein, a recombinant plasmid containing the nucleotide sequence, a recombinant cell containing the nucleotide sequence or the recombinant plasmid, and application of the mutant protein, the nucleotide sequence, the recombinant plasmid or the recombinant cell in the aspect of catalyzing and synthesizing taxol or analogues thereof.
In order to solve the technical problems of the invention, the following technical scheme is provided:
the first aspect of the technical scheme of the invention is as follows: there is provided a mutant protein of 10-deacetylbaccatin III 10 beta-O-acetyltransferase DBAT, characterized in that the mutant protein contains a mutation of R345C on the basis of the sequence shown in SEQ ID NO. 1.
In some preferred embodiments, the mutant protein further has one or more amino acid mutations selected from the group consisting of: G38R, V39A, V A, L123H, V158M, G163S, Q213R, C42216R, P217K, S219A, L E, S L, F301V, E350M, S351D, L383I, S396G, S37415L, M417L, S420L, I421V, V M.
In some preferred embodiments, the amino acid sequence of the mutant protein is a derivative polypeptide selected from the group consisting of the amino acid sequences shown in SEQ ID NO. 2-SEQ ID NO. 28, and having an homology of 85% (preferably 95%) to any one of the amino acid sequences shown in SEQ ID NO. 2-SEQ ID NO. 28, and having an acyltransferase activity.
In some preferred embodiments, conventional modifications may be made to the mutant proteins described above; the conventional modification comprises acetylation, amidation, cyclization, glycosylation, phosphorylation, alkylation, biotinylation, fluorescent group modification, polyethylene glycol PEG modification and immobilization modification;
alternatively, in some preferred embodiments, tags for detection or purification are attached to these mutant proteins; the label comprises 6X His, GST, EGFP, MBP, nus, HA, igG, FLAG, c-Myc and proficiency eXact.
The second aspect of the technical scheme of the invention is that: nucleotide sequences encoding the mutant proteins of the first aspect are provided, wherein the nucleotide sequences are SEQ ID NO 30-SEQ ID NO 56, and nucleotide sequences having a homology of 95% (preferably 98%) with any one of the sequences shown in SEQ ID NO 30-SEQ ID NO 56.
The third aspect of the technical scheme of the invention is that: providing a recombinant plasmid comprising the nucleotide sequence of the second aspect.
The fourth aspect of the technical scheme of the invention is that: providing a recombinant cell comprising the nucleotide sequence of the second aspect or the recombinant plasmid of the third aspect, said recombinant cell being constructed using a host cell comprising: coli, pichia, saccharomyces cerevisiae, aspergillus nidulans, bacillus subtilis or agrobacterium.
The fifth aspect of the technical scheme of the invention is that: providing the mutant protein of the first aspect, the nucleotide sequence of the second aspect, the recombinant plasmid of the third aspect and the recombinant cell of the fourth aspect for the application in the catalytic synthesis of taxol or analogues thereof; the acyl acceptors include, but are not limited to, 10-deacetyltaxol, 10-deacetylbaccatin III; the acyl donors include, but are not limited to, acetyl-CoA, propionyl-CoA and butyryl-CoA.
A preferred acyl donor substrate is acetyl coa and a preferred acyl acceptor substrate is 10-Deacetyltaxol (DT);
beneficial technical effects
The invention utilizes protein engineering to modify 10-deacetylbaccatin III 10 beta-O-acetyltransferase (DBAT) to obtain 27 mutant proteins with remarkably improved catalytic activity to unnatural acyl receptor substrate DT compared with DBATcus. The mutant proteins can catalyze DT to directly generate taxol with high efficiency. The invention can simplify the synthesis steps of the taxol or the analogues thereof, solves the problems of less taxol or analogues thereof resources and difficult synthesis, and has the advantage of environmental friendliness compared with a chemical semi-synthesis method.
Drawings
FIG. 1 is a differential site analysis of a DNA buffering template.
FIG. 2 is a schematic diagram of the basic principle of construction of DBAT mutants using DNA buffering technology.
FIG. 3 is an HPLC analysis of DBAT mutant cells for conversion of 10-DAB.
FIG. 4 is a schematic diagram of SDS-PAGE bands of the target protein.
FIG. 5 is an HPLC analysis of DBAT family mutant protein catalytic unnatural substrate DT.
FIG. 6 is an optimum temperature curve and an optimum pH curve.
FIG. 7 is a plot of product concentration versus time for 2-283-F301V/E350M catalytic DT.
Detailed Description
The invention is further illustrated by the following examples, which are intended to be illustrative only and are not intended to limit the scope of the claims in any way.
Example 1: construction of DBAT DNA shuffling mutant
By artificially synthesizing the dbat gene sequence (dbat) of taxus northeast cus GenBank Accession: Q9M6E2.1), taxus brevifolia origin dbat (dbat) bre GenBank Accession, EU 107143.1) G38R/F301V mutant Gene sequence (dbat) bre G38R/F301V ) The dbat gene sequence (dbat) of Taxus media med GenBank Accession AY 452666.1), and the dbat gene sequence (dbat) of Taxus canadensis can GenBank Accession EU 107134.1), the dbat gene sequence of Taxus baccata (dbat) bac GenBank Accession AF 456342.1), and dbat gene sequence (dbat) of Taxus media wal GenBank Accession:EU 107140.1) as template.
The differential site analysis of the 6 DBAT proteins is shown in figure 1. The upstream and downstream primers used are shown in Table 1.
TABLE 1.6 upstream and downstream primers for amplification of dbat Gene sequences
The PCR amplification system of the 6 dbat gene fragments is as follows:
the PCR amplification conditions were as follows:
the PCR product was detected by agarose gel electrophoresis at 1.0%, and the PCR product was purified and recovered.
The following procedure was followed as described for the BTN131178DNA buffering kit: the PCR product was fragmented and all fragments ranging from 25 to 150bp were recovered by 3.0% agarose gel electrophoresis. 500ng of the recovered fragments were subjected to primer-free PCR amplification. Purifying and recovering 1 kb-1.5 kb fragment by 1.0% agarose gel electrophoresis, and using the fragment as template and dbat cus F/R is the primer for final PCR amplification. The basic principle diagram of constructing DBAT mutant by using DNA shuffle technology is shown in figure 2.
After the final amplified fragment was recovered, the resultant was digested with Nde I and Sal I, ligated with the pCWori vector digested with the same double enzymes, E.coli Trans109 competent was obtained, and positive transformants were selected by colony PCR.
Example 2: prokaryotic expression of DBAT mutant protein and bacterial activity screening
Single colonies of each positive transformant obtained in example 1 were picked up in 96-well deep well plates, each well containing 500. Mu.L of TBA (final Amp concentration of 100. Mu.g/mL) liquid medium, and after shaking culture at 37℃and 220rpm for about 12 hours, the culture of each single colony was inoculated with 100. Mu.L of seed liquid: 900. Mu.L of fresh TBA medium was inoculated in parallel into 3 wells of a 96-well plate, and after shaking culture at 37℃and 220rpm for 1 hour, 1. Mu.L of 1M IPTG was added to each well, followed by shaking culture at 18℃and 220rpm for 20 hours, and the 96-well plate was centrifuged to collect the cells after induction culture.
Primary screening mutants with significantly improved catalytic activity on DT were screened in positive variant strains relative to wild type DBATcus using 10-DAB as substrate. To each well, 100. Mu.L of 50mM sodium acetate-acetic acid buffer (pH 5.5) was added as a control to resuspend the cells, then 2.5. Mu.L of 10mM 10-DAB (DMSO was dissolved) was added, and after shaking reaction at 40℃and 250rpm for 2 hours, 400. Mu.L of methanol was added to terminate the reaction, and 50. Mu.L of the detection transformation product was applied by HPLC (see FIG. 3).
Example 3: specific activity determination of DBAT mutant protein catalytic substrate DT
Selecting a mutant flat plate colony (positive variant strain) with the bacterial level catalytic activity higher than DBATcus obtained in the example 2, inoculating the colony into 10mL TBA (Amp final concentration is 100 mug/mL) liquid culture medium, and placing the culture medium at 37 ℃ and shaking at 220rpm for overnight; transferring the recombinant bacteria cultured overnight into 200mL of fresh TBA liquid culture medium according to the proportion of 1%, and culturing for 2-3 h at 37 ℃ and 220 rpm; waiting bacteria liquid OD 600 At approximately 0.8, IPTG was added to a final concentration of 1mM, inducing culture conditions: 18 ℃, 220rpm, 20h. And centrifuging and collecting the induced thalli.
The bacterial cells after induction of expression were resuspended in equilibration buffer (20 mM imidazole, 100mM NaCl, 20mM Tris-HCl, pH 7.5) at a ratio of 50mL buffer to 1L bacterial cell pellet collected, broken up at a high pressure of 800bar for 5 times, centrifuged at 12000rpm at 4℃for 1h, and the supernatant was filtered through a 0.45 μm filter, purified by HisTrap HP (5 mL) preloaded with nickel column, and the fractions eluted with 200mM imidazole buffer were collected (the band of the protein of interest is shown in FIG. 4). The obtained target protein eluent was concentrated by a ultrafiltration tube having a molecular weight cut-off (Molecular Weight Cutoff, MWCO) of 30kDa under centrifugation conditions of 4000g for 30min, and the protein concentration of the concentrated sample was measured.
The specific activity detection system of DBATcus and mutant catalytic DT thereof is as follows:
100. Mu.L of the reaction system contained 0.25mg/mL of protein, 500. Mu.M (404.5. Mu.g/mL) of acetyl CoA, 500. Mu.M (405.94. Mu.g/mL) of DT, 20mM of imidazole, 5% of DMSO (V/V), and 50mM of sodium acetate-acetic acid buffer solution at pH5.5 were used to make up to 100. Mu.L, after shaking reaction at 37.5℃for 3 hours, 400. Mu.L of methanol was added to terminate the reaction, and 50. Mu.L of the conversion product was detected by HPLC (see FIG. 5).
The specific activity of DBATcus and its mutant catalyzing DT is shown in Table 2, and the mutation site compared to DBATcus is shown in Table 3. As can be seen from the data in the table, the specific activity of the mutant catalytic DT is obviously improved compared with DBATcus, and the specific activity of the mutant catalytic DT can be up to 7.9 times.
TABLE 2 specific Activity and relative enzymatic Activity of DBATcus and its mutants catalyzing DT
TABLE 3 mutation sites of DNA stuffer mutants (compared to DBATcus)
Example 4: acquisition of DBAT combination mutant and determination of specific Activity of catalytic DT
The method of whole plasmid PCR amplification was used, using the respective mutant primers shown in Table 4:
taking pCWori-2-60 as a template, introducing F301V mutation, and constructing a 2-60-F301V combined mutant;
taking pCWori-2-181 as a template, respectively introducing G38R, P217K, F301V, E350M, S351D, S G mutation, constructing 2-181-G38R, 2-181-P217K, 2-181-F301V, 2-181-E350M, 2-181-S351D and 2-181-S396G combined mutant, and continuously introducing S396G mutation on the basis of pCWori-2-80-R345C to construct 2-80-R345C/S396G combined mutant;
the pCWori-2-283 is used as a template, and the G38R, P217K, F301V, E350M, S351D, S396G mutation is respectively introduced to construct 2-283-G38R, 2-283-P217K, 2-283-F301V, 2-283-E350M, 2-283-S351D and 2-283-S396G combined mutants;
P217K, E and 350M, S D mutation are respectively introduced on the basis of pCWori-2-283-F301V to construct 2-283-F301V/P217K, 2-283-F301V/E350M and 2-283-F301V/S351D combined mutants;
in addition, P217K, S D mutation is continuously introduced by taking pCWori-2-283-F301V/E350M as a template, and 2-283-F301V/E350M/P217K, 2-283-F301V/E350M/S351D mutants and 2-283-F301V/E350M/P217K/S351D mutants are constructed.
TABLE 4 upstream and downstream primers for introducing the mutant into the respective mutation sites
The PCR system for whole plasmid amplification was as follows:
the PCR amplification conditions were as follows:
the PCR product was detected by agarose gel electrophoresis at 1.0%, and the PCR product was purified and recovered.
The PCR product was digested with Dpn I, and the digestion system was as follows:
enzyme cutting conditions: and enzyme cutting at 37 ℃ for 5 hours.
All the cleavage products were transformed into competent cells of E.coli Trans 109. Colonies were picked for plasmid extraction and sequencing validation.
The mutants were subjected to protein-induced expression, purification and specific activity determination of catalytic substrate DT as described in example 3, and the results are shown in Table 5, in which the activity of the combined mutants was significantly improved, up to 11.23 times, compared to DBATcus.
TABLE 5 specific Activity and relative enzymatic Activity of the combined mutant protein catalytic DT
TABLE 6 mutation sites of the combination mutants (compared to DBATcus)
Example 5: determination of optimal reaction conditions for DBAT mutant protein catalytic substrate DT
The enzyme catalysis system is the same as in example 3.
The determination of the optimum reaction pH was carried out at 37.5℃and the buffer composition and pH were set to 50mM sodium acetate-acetic acid buffer (pH 4.0, 4.5, 5.0, 5.5, 6.0, respectively) and 50mM sodium phosphate buffer (pH 6.5, 7.0, 7.5, 8.0, respectively).
The optimum reaction temperature was measured at pH5.5, and the temperatures were set to 25, 27.5, 30, 32.5, 35, 37.5, 40, 42.5, 45, 47.5, and 50 (. Degree.C.).
The results are shown in FIG. 6 and Table 7.
TABLE 7 optimum reaction conditions for DBATcus and several highly active mutant protein catalytic substrates DT
Example 6: dynamic parameter determination of DBATcus and mutant protein catalytic substrate DT
The catalytic system comprises: the 100. Mu.L reaction system comprises 0.5mg/mL of protein, 1mM of acetyl CoA, different concentrations of DT, 20mM of imidazole and 5% of DMSO (V/V), the different proteins are respectively supplemented to 100. Mu.L by using buffers with optimal pH, and the reaction is carried out by shaking for 1h at the respective optimal temperatures, and the conversion products are detected by HPLC.
The final DT concentrations were 2000, 1000, 500, 250, 125, 62.5, 31.25, 15.625. Mu.M, respectively.
The results are shown in Table 8, where the conversion efficiency of 2-60 is 7.2 times that of DBATcus, and the conversion efficiency of 2-283-P217K/F301V/E350M/S351D is 13.99 times that of DBATcus.
TABLE 8 kinetic parameters of DBATcus and mutant protein catalytic substrate DT
Example 7: product time-concentration profile for 2-283-F301V/E350M catalytic DT
The catalytic system comprises: 1mL of the reaction system contains 1mg/mL of protein, 10mM of acetyl-CoA, 10mM of DT, 20mM of imidazole, 5% of DMSO (V/V), and 50mM of sodium acetate-acetic acid buffer pH5.5, which is supplemented to 1mL. The production of Taxol was measured at 3h, 6h, 9h, 12h, 24h, 27h, 30h, 33h and 36h, respectively, and when the reaction was carried out for 12h, 1mg/mL final protein and 10mM final acetyl CoA were added to the system.
Reaction conditions: 32.5℃pH5.5, 600rpm.
The results are shown in fig. 7, which shows:
under the catalysis of 2-283-F301V/E350M, the yield of taxol increases at a relatively high speed in the first 6h of reaction, the yield increasing speed is gradually reduced from 6h to 12h, the reaction is continued for 24h after the protein is added in the 12h, and the highest yield of taxol is 2.27+/-0.04 mg/mL.
Sequence listing
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<213> Artificial Sequence
<400> 3
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Arg Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Ser Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile His Pro Pro Thr Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Leu Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 4
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 4
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 5
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 5
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Ile Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Val Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 6
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 6
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Ala Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Ile Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 7
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 7
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Leu Phe
405 410 415
Leu Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 8
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 8
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Ala Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile His Pro Pro Thr Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Leu Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 9
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 9
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 10
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 10
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 11
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 11
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Arg Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Leu Phe
405 410 415
Leu Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 12
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 12
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Lys Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Leu Phe
405 410 415
Leu Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 13
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 13
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Leu Phe
405 410 415
Leu Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 14
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 14
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Met Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Leu Phe
405 410 415
Leu Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 15
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 15
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Asp Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Leu Phe
405 410 415
Leu Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 16
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 16
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Gln Leu Ile Cys Pro Pro Ser Thr Phe Gly Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Gly Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Leu Phe
405 410 415
Leu Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 17
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 17
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Arg Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 18
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 18
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Lys Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 19
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 19
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 20
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 20
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Met Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 21
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 21
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Asp Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 22
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 22
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Phe Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Gly Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 23
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 23
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Lys Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 24
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 24
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Met Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 25
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 25
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Glu Asp Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 26
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 26
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Lys Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Met Ser Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 27
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 27
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Pro Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Met Asp Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 28
<211> 440
<212> PRT
<213> Artificial Sequence
<400> 28
Met Ala Gly Ser Thr Glu Phe Val Val Arg Ser Leu Glu Arg Val Met
1 5 10 15
Val Ala Pro Ser Gln Pro Ser Pro Lys Ala Phe Leu Gln Leu Ser Thr
20 25 30
Leu Asp Asn Leu Pro Gly Val Arg Glu Asn Ile Phe Asn Thr Leu Leu
35 40 45
Val Tyr Asn Ala Ser Asp Arg Val Ser Val Asp Pro Ala Lys Val Ile
50 55 60
Arg Gln Ala Leu Ser Lys Val Leu Val Tyr Tyr Ser Pro Phe Ala Gly
65 70 75 80
Arg Leu Arg Lys Lys Glu Asn Gly Asp Leu Glu Val Glu Cys Thr Gly
85 90 95
Glu Gly Ala Leu Phe Val Glu Ala Met Ala Asp Thr Asp Leu Ser Val
100 105 110
Leu Gly Asp Leu Asp Asp Tyr Ser Pro Ser Leu Glu Gln Leu Leu Phe
115 120 125
Cys Leu Pro Pro Asp Thr Asp Ile Glu Asp Ile His Pro Leu Val Val
130 135 140
Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val Ser Phe
145 150 155 160
Cys His Gly Ile Cys Asp Gly Leu Gly Ala Gly Gln Phe Leu Ile Ala
165 170 175
Met Gly Glu Met Ala Arg Gly Glu Ile Lys Pro Ser Ser Glu Pro Ile
180 185 190
Trp Lys Arg Glu Leu Leu Lys Pro Glu Asp Pro Leu Tyr Arg Phe Gln
195 200 205
Tyr Tyr His Phe Arg Leu Ile Arg Lys Pro Ser Thr Phe Glu Lys Ile
210 215 220
Val Gln Gly Ser Leu Val Ile Thr Ser Glu Thr Ile Asn Cys Ile Lys
225 230 235 240
Gln Cys Leu Arg Glu Glu Ser Lys Glu Phe Cys Ser Ala Phe Glu Val
245 250 255
Val Ser Ala Leu Ala Trp Ile Ala Arg Thr Arg Ala Leu Gln Ile Pro
260 265 270
His Ser Glu Asn Val Lys Leu Ile Phe Ala Met Asp Met Arg Lys Leu
275 280 285
Phe Asn Pro Pro Leu Ser Lys Gly Tyr Tyr Gly Asn Val Val Gly Thr
290 295 300
Val Cys Ala Met Asp Asn Val Lys Asp Leu Leu Ser Gly Ser Leu Leu
305 310 315 320
Arg Val Val Arg Ile Ile Lys Lys Ala Lys Val Ser Leu Asn Glu His
325 330 335
Phe Thr Ser Thr Ile Val Thr Pro Cys Ser Gly Ser Asp Met Asp Ile
340 345 350
Asn Tyr Glu Asn Ile Val Gly Phe Gly Asp Arg Arg Arg Leu Gly Phe
355 360 365
Asp Glu Val Asp Phe Gly Trp Gly His Ala Asp Asn Val Ser Leu Val
370 375 380
Gln His Gly Leu Lys Asp Val Ser Val Val Gln Ser Tyr Phe Leu Phe
385 390 395 400
Ile Arg Pro Pro Lys Asn Asn Pro Asp Gly Ile Lys Ile Leu Ser Phe
405 410 415
Met Pro Pro Ser Ile Val Lys Ser Phe Lys Phe Glu Met Glu Thr Met
420 425 430
Thr Asn Lys Tyr Val Thr Lys Pro
435 440
<210> 29
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 29
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtagg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
atcgtgacac cccgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 30
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 30
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacatg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg gatgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagcca 600
gaagaccctt tatatcggtt ccagtattat cactttcgat tgattcaccc gcctacgaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagattttg ggtggggaca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtta 1260
ataatgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 31
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 31
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc acgggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtagg ggtgagtttc 480
tgccatagta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcaccc gcctacgaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtttctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagattttg ggtggggaca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtta 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 32
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 32
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacatctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtggggaca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 33
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 33
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc tgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtagg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtattaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatc 840
tttgcaatgg acatgaggaa attatttaat ccaccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtggggaca tgcagataat 1140
gtaagtatcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
gtagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 34
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 34
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc tgcagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacatctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatc 840
tttgcaatgg acatgaggaa attatttaat ccaccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
atcgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtggggaca tgcagataat 1140
gtaagtatcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 35
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 35
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tattgttcct gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 36
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 36
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggcgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcaccc gcctacgaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt tgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtggggaca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 37
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 37
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 38
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 38
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacatctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
gtcgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtggggaca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 39
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 39
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aagagtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tattgttcct gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 40
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 40
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgcaa gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tattgttcct gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 41
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 41
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
gtggttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tattgttcct gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 42
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 42
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatatg agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tattgttcct gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 43
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 43
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag gacatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tattgttcct gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 44
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 44
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggagatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgactacagt 360
ccttcacttg agcaactact tttttgtctt ccacctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaaggggag agattaagcc ctcctcggag ccaatatgga agagagaatt gctgaagccg 600
gaagaccctt tataccggtt ccagtattat cactttcaat tgatttgccc gccttcaaca 660
ttcgggaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacaag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgaggaa attatttaat ccgccacttt cgaagggata ctacggtaat 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaggata ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tattgttcct gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 45
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 45
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aagagtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 46
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 46
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgcaa accttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 47
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 47
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
gttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 48
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 48
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatatg agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 49
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 49
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag gatatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 50
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 50
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
tttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaggata ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 51
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 51
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgcaa gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
gttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 52
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 52
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
gttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatatg agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 53
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 53
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
gttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatgag gatatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 54
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 54
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgcaa gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
gttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatatg agtatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 55
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 55
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgccc gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
gttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatatg gatatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323
<210> 56
<211> 1323
<212> DNA
<213> Artificial Sequence
<400> 56
atggcaggct caacagaatt tgtggtaaga agcttagaga gagtgatggt ggctccaagc 60
cagccatcgc ccaaagcttt cctgcagctc tccacccttg acaatctacc aggggtgaga 120
gaaaacattt ttaacacctt gttagtctac aatgcctcag acagagtttc cgtagatcct 180
gcaaaagtaa ttcggcaggc tctctccaag gtgttggtgt actattcccc ttttgcaggg 240
cgtctcagga aaaaagaaaa tggggatctt gaagtggagt gcacagggga gggtgctctg 300
tttgtggaag ccatggctga cactgacctc tcagtcttag gagatttgga tgattacagt 360
ccttcacttg agcaactact tttttgtctt ccgcctgata cagatattga ggacatccat 420
cctctggtgg ttcaggtaac tcgttttaca tgtggaggtt ttgttgtggg ggtgagtttc 480
tgccatggta tatgtgatgg actaggagca ggccagtttc ttatagccat gggagagatg 540
gcaagggggg agattaagcc ctcctcggag ccgatatgga agagagaatt gctgaagcca 600
gaagaccctt tataccggtt ccagtattat cactttcgat tgattcgcaa gccttcgaca 660
ttcgagaaaa tagttcaagg atctcttgtt ataacctctg agacaataaa ttgtatcaaa 720
caatgcctta gggaagaaag taaagaattt tgctctgcgt tcgaagttgt atctgcattg 780
gcttggatag caaggacgag ggctcttcaa attccacata gtgagaatgt gaagcttatt 840
tttgcaatgg acatgagaaa attatttaat ccaccacttt cgaagggata ctacggtaac 900
gttgttggta ccgtatgtgc aatggataat gtcaaggacc tattaagtgg atctcttttg 960
cgtgttgtaa ggattataaa gaaagcaaag gtctctttaa atgagcattt cacgtcaaca 1020
attgtgacac cctgttctgg atcagatatg gatatcaatt atgaaaacat agttggattt 1080
ggtgatcgaa ggcgattggg atttgatgaa gtagactttg ggtgggggca tgcagataat 1140
gtaagtctcg tgcaacatgg attgaaggat gtttcagtcg tgcaaagtta ttttcttttc 1200
atacgacctc ccaagaataa ccccgatgga atcaagatcc tatcgttcat gcccccgtca 1260
atagtgaaat ccttcaaatt tgaaatggaa accatgacaa acaaatatgt aactaagcct 1320
tga 1323

Claims (13)

1. A mutant protein of 10-deacetylbaccatin iii 10 beta-O-acetyltransferase DBAT, wherein the amino acid sequence of said mutant protein has at least an R345C amino acid mutation compared to the wild type protein DBAT shown in SEQ ID NO 1.
2. The mutant protein of claim 1, wherein the mutant protein further has one or more amino acid mutations selected from the group consisting of: G38R, V39A, V A, L123H, V158M, G163S, Q213R, C42216R, P217K, S219A, L E, S L, F301V, E350M, S351D, L383I, S396G, S37415L, M417L, S420L, I421V, V M.
3. Mutant protein according to claim 1 or 2, wherein the amino acid sequence of the mutant protein is selected from the group consisting of the amino acid sequences shown in SEQ ID NO 2 to SEQ ID NO 28, and derived polypeptides having homology of more than or equal to 85%, preferably more than or equal to 95%, with any one of the amino acid sequences shown in SEQ ID NO 2 to SEQ ID NO 28 and having an acyltransferase activity.
4. A mutant protein according to any one of claims 1 to 3, wherein the mutant protein further has one or more modifications selected from the group consisting of: acetylation, amidation, cyclization, glycosylation, phosphorylation, alkylation, biotinylation, fluorophore modification, polyethylene glycol PEG modification, immobilization modification.
5. The mutant protein according to any one of claims 1 to 4, wherein the mutant protein is further linked to one or more tags selected from the group consisting of: 6X His, GST, EGFP, MBP, nus, HA, igG, FLAG, c-Myc, proficiency eXact.
6. A polynucleotide encoding the mutant protein of any one of claims 1-5.
7. The polynucleotide according to claim 6, wherein the polynucleotide is selected from the group consisting of the sequences shown in SEQ ID NO30 to SEQ ID NO 56, and nucleotide sequences having a homology of 95% or more, preferably a homology of 98% or more with the sequence shown in any one of SEQ ID NO30 to SEQ ID NO 56.
8. A recombinant plasmid comprising the polynucleotide of claim 6 or 7.
9. A recombinant cell incorporating the polynucleotide of any one of claims 6 or 7 or the recombinant plasmid of claim 8.
10. The recombinant cell of claim 9, wherein the recombinant cell is constructed using a host cell that: coli, pichia, saccharomyces cerevisiae, aspergillus nidulans, bacillus subtilis or agrobacterium.
11. Use of a mutant protein according to any one of claims 1 to 5 or a nucleotide sequence according to claim 6 or 7 or a recombinant plasmid according to claim 8 or a recombinant cell according to claim 9 for catalyzing the acylation of 10-deacetyltaxane to 10-acyl taxane.
12. The use according to claim 11, wherein said 10-deacetyltaxane comprises 10-deacetyl taxol, 10-deacetyl baccatin iii.
13. The use according to claim 11 or 12, wherein the acyl donor comprises acetyl coa, propionyl coa and butyryl coa.
CN202210444105.0A 2022-04-26 2022-04-26 10-deacetylbaccatin III 10 beta-O-acetyltransferase mutant and application thereof Pending CN116987681A (en)

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