CN116948783B - Device and method for enriching active ingredients in bone marrow blood - Google Patents
Device and method for enriching active ingredients in bone marrow blood Download PDFInfo
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- CN116948783B CN116948783B CN202310926281.2A CN202310926281A CN116948783B CN 116948783 B CN116948783 B CN 116948783B CN 202310926281 A CN202310926281 A CN 202310926281A CN 116948783 B CN116948783 B CN 116948783B
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- 239000004480 active ingredient Substances 0.000 title claims abstract description 100
- 210000004369 blood Anatomy 0.000 title claims abstract description 68
- 239000008280 blood Substances 0.000 title claims abstract description 68
- 210000001185 bone marrow Anatomy 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000002347 injection Methods 0.000 claims abstract description 38
- 239000007924 injection Substances 0.000 claims abstract description 38
- 239000000463 material Substances 0.000 claims description 23
- 238000007789 sealing Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 abstract description 12
- 230000037452 priming Effects 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 210000000988 bone and bone Anatomy 0.000 description 21
- 238000001914 filtration Methods 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 13
- 210000000130 stem cell Anatomy 0.000 description 6
- 230000007547 defect Effects 0.000 description 4
- 206010044565 Tremor Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000011164 ossification Effects 0.000 description 2
- 241001529936 Murinae Species 0.000 description 1
- 206010031252 Osteomyelitis Diseases 0.000 description 1
- 238000011579 SCID mouse model Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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Abstract
The invention provides a marrow blood active ingredient enrichment device and an enrichment method, wherein a second channel with a second one-way valve is arranged at the periphery of an active ingredient collection part of the enrichment device, and the second channel is communicated with a variable cavity and a containing cavity of an injection and suction mechanism; operating the priming mechanism to cause bone marrow blood to flow from the first channel to the variable chamber and back from the second channel; the enrichment method comprises the following steps: the exhaust valve is opened first, then the second piston component of the injection and suction mechanism is pushed forward, so that bone marrow blood enters the variable cavity after passing through the active component collecting part, and the second piston component of the injection and suction mechanism is pulled backward, so that bone marrow blood in the variable cavity flows back into the accommodating cavity of the injection and suction mechanism through the second channel. In the invention, the one-way and reciprocating circulation of the marrow blood can be smoothly completed only by a short stroke, the waste amount of the marrow blood is very small, and the cell activity can be considered on the basis of ensuring the enrichment efficiency.
Description
Technical Field
The invention relates to the technical field of enrichment of active ingredients such as bone marrow blood cells, growth factors and the like, in particular to a device and a method for enriching active ingredients of bone marrow blood.
Background
The prior document CN113862130a discloses a stem cell filtration concentrator comprising: the filter device is internally provided with an artificial bone material and is used for enriching stem cells and is communicated with the outside; one end of the first pipeline is communicated with the filtering device, and the other end of the first pipeline is connected with a syringe which is used for containing bone marrow blood; one end of the second pipeline is communicated with one end of the filtering device, which is far away from the second pipeline, and the other end of the second pipeline is communicated with the first pipeline; the first one-way valve is positioned at one end of the first pipeline close to the filtering device and is used for controlling the liquid to flow from the first pipeline to the filtering device; and the second one-way valve is positioned at one end of the second pipeline close to the first pipeline and is used for controlling the liquid to flow from the second pipeline to the first pipeline.
The stem cell filtration enriching device adopts a technical route of unidirectional enrichment, bone marrow blood enters the filtering device through the first one-way valve, artificial bone materials in the filtering device are enriched, filtered blood enters the bottom of the filtering device under the pushing of the syringe, after the whole bone marrow blood is filtered, the syringe is pulled back, the blood accumulated at the bottom of the filtering box is pumped into the second pipeline, enters the first pipeline through the second one-way valve, and flows back into the syringe. However, the second pipeline, the second connector, the second one-way valve, the first one-way valve, the inner cavity of the shell and the third connector of the stem cell filtration enrichment device form a fixed closed loop path together, and the injector is pulled back very hard, so that the enrichment function cannot be realized in the use process.
More importantly, the existing stem cell filtration enriching device is only suitable for the condition that the bone marrow blood consumption is large (more than 20mL of volume), and in some special cases (such as dental bone grafting, spinal interbody fusion cage bone grafting and the like), the required filling material is very small (0.5 mL of filling material), and correspondingly, the bone marrow blood is relatively small (2-2.5 mL of bone marrow blood). However, there is currently a lack of enrichment devices suitable for rapid enrichment of trace volumes (2-10 mL) of bone marrow blood active ingredients. In addition, the stem cell filtration enriching device has the problems of not compact structure and inconvenient disassembly and assembly of the filtration device. In addition, the existing enricher has the problem of easily influencing the cell activity, and how to consider the cell activity on the basis of ensuring the enrichment efficiency is also a problem to be solved.
Disclosure of Invention
The invention aims to provide a marrow blood active ingredient enrichment device and an enrichment method, which at least can solve the technical problem of how to carry out active ingredient enrichment rapidly, flexibly and unidirectionally aiming at extracted trace marrow blood.
The invention adopts the following technical scheme.
The active ingredient enrichment device of the bone marrow blood comprises an active ingredient collecting part, wherein an injection and suction mechanism is arranged at the left side of the active ingredient collecting part, the injection and suction mechanism can suck the bone marrow blood and inject the bone marrow blood into the active ingredient collecting part, the active ingredient collecting part is arranged in a shell, a piston is matched at the right side of the active ingredient collecting part, and a variable cavity of an inner cavity of the shell is communicated with the active ingredient collecting part; the active ingredient collecting part is provided with a second channel with a second one-way valve at the periphery, and the second channel is communicated with the variable cavity and the accommodating cavity of the injection and suction mechanism; operating the priming mechanism to cause bone marrow blood to flow from the first channel to the variable chamber and back from the second channel; wherein, be provided with the discharge valve on the second passageway.
Preferably, the active ingredient collecting part comprises a cylinder body and at least two filter screens arranged on the cylinder body, and at least two filter screens and the inner wall of the cylinder body jointly form a space for storing the enrichment filling material. More preferably, the active ingredient collecting part comprises a cylinder body and two filter screens arranged at two ends of the cylinder body, and a space for storing the enriched filling material is jointly formed by the two filter screens and the inner wall of the cylinder body.
In order to collect and distribute the active ingredients in the enriched filling material more uniformly, the filter screen at the left end of the active ingredient collecting part is protruded to the left side so that the surface of the filter screen is conical.
In order to further improve the stability of the enrichment device, the active ingredient collecting part is clamped on the mounting part, the mounting part is in threaded fit on the shell, the mounting part is provided with a shaft hole, the second channel is communicated with the shaft hole and is positioned on the mounting part, the right end of the active ingredient collecting part is level with the right end of the mounting part, and the shaft hole is used for being matched with an inserting port of the injection mechanism.
Preferably, a sealing element is provided at the left end of the cylinder, and the sealing element is used to seal the connection portion between the active ingredient collecting portion and the mounting portion.
In order to further reduce the amount of bone marrow blood, the right end of the active ingredient collection part is abutted against the left end of the variable chamber.
In order to further improve the stability of the enrichment device, prevent the active ingredient collecting part from trembling in the use process, and in order to more conveniently and smoothly take and mount the active ingredient collecting part, a step is arranged in the shell and positioned at the left end of the variable chamber, and the active ingredient collecting part is axially limited by means of the step.
The second channel is provided with a vertical section and a horizontal section, the vertical section is vertical to the shaft hole, the horizontal section is parallel to the shaft hole, the exhaust valve is arranged at the joint part of the vertical section and the horizontal section, and when the exhaust valve is opened, the valve holes of the horizontal section and the exhaust valve are used as exhaust channels. By adopting the scheme, in the process of repeatedly drawing and pushing the second piston assembly, not only the residual bone marrow blood in the second channel can be well prevented, but also the rapid and flexible enrichment of the active ingredients of the bone marrow blood with the micro volume (2-10 mL) is facilitated.
An enrichment method adopting the active ingredient enrichment device comprises the following steps:
step 1, pulling a second piston assembly of the injection and suction mechanism backwards to suck bone marrow blood into a containing cavity of the injection and suction mechanism;
step 2, connecting the injection and suction mechanism on the shell;
step 3, firstly opening the exhaust valve, then pushing the second piston assembly of the injection and suction mechanism forwards to enable bone marrow blood to enter the variable cavity after passing through the active ingredient collecting part, in the process, the piston moves rightwards, the volume of the variable cavity is increased, and then closing the exhaust valve;
step 4, pulling the second piston assembly of the injection and suction mechanism backwards to enable the bone marrow blood in the variable cavity to flow back into the accommodating cavity of the injection and suction mechanism through the second channel 8;
and 5, repeatedly pushing and pulling the second piston assembly until the enrichment filling material with the active ingredients is taken out after the enrichment is completed.
The beneficial effects are that: in the invention, only a short stroke is needed to smoothly complete unidirectional and reciprocating circulation of the bone marrow blood, the waste amount of the bone marrow blood is very small, and by adopting the structure and the mode, the 2-10ml bone marrow blood can be rapidly and flexibly enriched with active ingredients, the operation of the active ingredient enrichment is simple, convenient and handy, the active ingredient collecting part can be disassembled and assembled conveniently, and the unidirectional and reciprocating circulation of the bone marrow blood can be realized by pushing and pulling the second piston component of the injection and suction mechanism forwards during the operation; the marrow blood active ingredient enrichment device has the advantages of small and compact structure, portability and low manufacturing cost; the enrichment device of the marrow blood active ingredient can also give consideration to cell activity on the basis of ensuring enrichment efficiency, the enrichment rate of the enrichment device of the specific structure of the invention on nucleated cells of marrow blood can reach up to about 85%, the enrichment device has no significant influence on the cell activity before and after enrichment, and the activity is above 98%.
Drawings
FIG. 1 is a schematic view showing the external structure of an active ingredient enrichment device (without a priming mechanism) in example 1;
FIG. 2 is a cross-sectional view of the active ingredient enrichment device of example 1;
FIG. 3 is a schematic cross-sectional view of the active ingredient enrichment device of example 1 in use;
FIG. 4 is a schematic diagram of the active ingredient collection portion of example 1;
FIG. 5 is a second schematic view of the active ingredient collection portion of example 1;
FIG. 6 is a schematic cross-sectional view of an active ingredient collection portion of example 1;
FIG. 7 is an exploded view of the active ingredient enrichment device of example 1;
fig. 8 is a view of an unenriched bone repair material set, fig. 9 is a view of 3D and 2D central sagittal views of bone tissue of an unenriched bone repair material set and an osteomyelitis-enriched bone repair material set in a bone defect model, with a scale of 1cm.
Detailed Description
The following description of the embodiments of the present invention will be apparent and complete, and it is to be understood that the embodiments described are merely some, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Referring to fig. 1 to 7, a bone marrow blood active ingredient enrichment device comprises an active ingredient collection part 3, wherein an injection and suction mechanism 1 is arranged at the left side of the active ingredient collection part 3, the injection and suction mechanism 1 can suck bone marrow blood and inject the bone marrow blood into the active ingredient collection part 3, the active ingredient collection part 3 is arranged in a shell 2, a piston 4 is matched at the right side of the active ingredient collection part 3, a variable cavity 7 in the inner cavity of the shell 2 is communicated with the active ingredient collection part 3, and the right end of the active ingredient collection part 3 is abutted against the left end of the variable cavity 7; the device mainly comprises a first channel formed by a containing cavity of the injection and suction mechanism 1, an inner cavity of the active ingredient collecting part 3 and a first one-way valve 5, wherein a second channel 8 with a second one-way valve 6 is arranged at the periphery of the active ingredient collecting part 3, and the second channel 8 is communicated with a variable cavity 7 and the containing cavity of the injection and suction mechanism 1; operation of the aspiration mechanism 1 enables bone marrow blood to flow from the first channel to the variable chamber 7 and back from the second channel 8. In this embodiment, the key is that the second channel 8 has a vertical section and a horizontal section, the vertical section is perpendicular to the shaft hole 35, the horizontal section is parallel to the shaft hole 35, the exhaust valve 13 is installed at the joint (intersection) position of the vertical section and the horizontal section, and when the exhaust valve 13 is opened, the valve hole of the horizontal section and the exhaust valve 13 is used as an exhaust channel, the right end of the horizontal section is directly connected with the second one-way valve 6, the second one-way valve 6 is directly communicated with the gap between the variable chamber 7 and the active ingredient collecting part 3, by adopting such a scheme, in the process of repeatedly drawing and pushing the second piston assembly 11, not only the residual bone marrow blood in the second channel 8 can be well prevented, but also the quick and flexible enrichment of the trace volume (2-10 mL) of bone marrow blood active ingredient is facilitated.
As shown in fig. 4 to 6, the active ingredient collecting portion 3 includes a cylindrical body 31 and two screens 32 provided at both ends of the cylindrical body 31, and a space for storing the enriched filler 33 is formed by the two screens 32 and the inner wall of the cylindrical body 31.
The screen 32 at the left end of the active ingredient collecting part 3 is protruded to the left so that the surface of the screen 32 is tapered, and the active ingredient can be more uniformly collected and distributed in the enrichment filling material by adopting the structure. The active ingredient collecting part 3 is clamped on the mounting part 30, the mounting part 30 is in threaded fit on the shell 2, a shaft hole 35 is formed in the mounting part 30, the second channel 8 is communicated with the shaft hole 35 and is located on the mounting part 30, the right end of the active ingredient collecting part 3 is flush with the right end of the mounting part 30, and the shaft hole 35 is used for being matched with the plug-in port 10 of the injection mechanism 1. The left end of the cylinder 31 is provided with a sealing member, and the connection portion between the active ingredient collecting portion 3 and the mounting portion 30 is sealed by the sealing member.
Wherein a step 34 is provided in the housing 2 at the left end of the variable chamber 7, and the active ingredient collection portion 3 is axially restrained by the step 34. The structure can further improve the stability of the enrichment device, prevent the active ingredient collecting part from trembling in the use process, and is beneficial to conveniently and smoothly taking and loading the active ingredient collecting part.
In this embodiment, the mating surface of the active ingredient collection portion 3 and the mounting portion 30 also has an inclined surface so that the active ingredient collection portion 3 can be inserted and removed more smoothly.
An enrichment method adopting the active ingredient enrichment device in the embodiment comprises the following steps:
step 1, pulling the second piston assembly 11 of the injection and suction mechanism 1 backwards to pump bone marrow blood into a containing cavity of the injection and suction mechanism 1;
step 2, connecting the injection and suction mechanism 1 to the shell 2;
step 3, firstly opening the exhaust valve 13 (the state at this time can be seen in the corresponding part of fig. 2), then pushing the second piston assembly 11 of the injection mechanism 1 forward to make the bone marrow blood enter the variable chamber 7 after passing through the active ingredient collecting part 3, in this process, the piston 4 moves rightward, the volume of the variable chamber 7 becomes larger, the state at this time is seen in fig. 3, and then closing the exhaust valve 13 (the state at this time can be seen in the corresponding part of fig. 3);
step 4, pulling the second piston assembly 11 of the injection and suction mechanism 1 backwards to enable the bone marrow blood in the variable cavity 7 to flow back into the accommodating cavity of the injection and suction mechanism 1 through the second channel 8;
and 5, repeatedly pushing and pulling the second piston assembly 11 until the enriched filling material 33 with the active ingredients is taken out after the enrichment is completed. When the active ingredient collection portion 3 is required to be taken out, the mounting portion 30 is only required to be unscrewed and withdrawn, and then the active ingredient collection portion 3 is required to be taken out, which is very convenient.
Enrichment efficiency evaluation: the bone repair material was filled into the active ingredient collection portion 3 of the enrichment device, and the enrichment was circulated 5 times (steps 3 and 4 were repeated 5 times) using 10mL of bone marrow blood, taking about 3-5 minutes. The adsorption capacity (enrichment rate) of the bone repair material to the cells in the bone marrow blood was analyzed by a conventional blood test (Sysmex-xn 1000 blood analyzer, JPN) by measuring the density and cell activity of the nucleated cells in the bone marrow blood before and after enrichment using a cell counter (CytoSmart Exact FL, corning, USA), thereby evaluating the enrichment efficiency of the enrichment device, the results are shown in Table 1,
TABLE 1 results of cell Activity and cell number before and after enrichment
The result shows that the enrichment device has the enrichment rate of nucleated cells of the bone marrow blood up to about 85%, and has no significant influence on the cell activity before and after enrichment, and the activity is above 98%.
Bone formation effect: to further evaluate the bone-promoting effect of the material prepared by the enrichment device in this example, bone repair material enriched with bone marrow blood was implanted into a model of the femoral large segment defect of SCID mice, and the bone formation at the bone defect after 4 weeks of the implantation experiment was evaluated, and the results are shown in fig. 8 and 9.
The results showed that, 4 weeks after implantation, little new bone tissue was seen in the non-enriched bone repair material group in the SCID murine femur large segment bone defect model; the bone repair material group enriched with bone marrow blood can be used for realizing bone bridging and bone marrow cavity recanalization in the middle to achieve a bone healing state. The in vivo experiment shows that the bone repair material prepared by the bone marrow enrichment device has obvious bone promoting effect.
Example 2
A bone marrow blood active ingredient enrichment device, referring to example 1, which is mainly different from the example in that: the second channel 8 has a vertical section and an inclined section, the inclined section is close to the vertical section, the vertical section is perpendicular to the shaft hole 35, and an included angle between the vertical section and the inclined section is 85 degrees. With this arrangement, bone marrow blood remaining in the second channel 8 can be almost avoided during repeated drawing and pushing of the second piston assembly 11.
In the present invention, the arrangement of the second channel 8 and the active ingredient collection portion 3 is also very critical, which is advantageous in preventing bone marrow blood from remaining and greatly shortening the circulation path of bone marrow blood.
In the invention, when in use, the one-way and reciprocating circulation of the marrow blood can be smoothly completed only by a short stroke, the waste amount of the marrow blood is very small, and by adopting the structure and the mode, the 2-10ml of marrow blood can be rapidly and flexibly enriched with active ingredients, the operation of the active ingredient enrichment is simple, convenient and handy, the active ingredient collecting part can be disassembled and assembled conveniently, and the one-way and reciprocating circulation of the marrow blood can be realized by pushing and pulling the second piston component of the injection and suction mechanism forwards during the operation; the marrow blood active ingredient enrichment device has the advantages of small and compact structure, portability and low manufacturing cost.
Claims (3)
1. The utility model provides a marrow blood active ingredient enrichment device, includes active ingredient collection portion (3), is provided with in active ingredient collection portion (3) left side and annotates suction mechanism (1), annotates suction mechanism (1) and can aspirate marrow blood and infuse active ingredient collection portion (3), its characterized in that: the active ingredient collecting part (3) is arranged in the shell (2), the piston (4) is matched on the right side of the active ingredient collecting part (3), and the variable cavity (7) in the inner cavity of the shell (2) is communicated with the active ingredient collecting part (3); the injection and suction mechanism mainly comprises a first channel formed by a containing cavity of the injection and suction mechanism (1), an inner cavity of the active ingredient collecting part (3) and a first one-way valve (5) runner, wherein a second channel (8) with a second one-way valve (6) is arranged outside the active ingredient collecting part (3), and the second channel (8) is communicated with a variable cavity (7) and the containing cavity of the injection and suction mechanism (1); operating the injection and suction mechanism (1) can enable bone marrow blood to flow from the first channel to the variable chamber (7) and flow back from the second channel (8); wherein, the second channel (8) is provided with an exhaust valve (13);
the active ingredient collecting part (3) comprises a cylinder body (31) and two filter screens (32) arranged at two ends of the cylinder body (31), and a space for storing an enrichment filling material (33) is formed by the two filter screens (32) and the inner wall of the cylinder body (31);
the active ingredient collecting part (3) comprises a cylinder body (31) and at least two filter screens (32) arranged on the cylinder body (31), and at least two filter screens (32) and the inner wall of the cylinder body (31) jointly form a space for storing an enrichment filling material (33);
the filter screen (32) at the left end of the active ingredient collecting part (3) protrudes leftwards so that the surface of the filter screen (32) presents a conical surface;
the active ingredient collecting part (3) is clamped on the mounting part (30), the mounting part (30) is in threaded fit on the shell (2), the mounting part (30) is provided with a shaft hole (35), the second channel (8) is communicated with the shaft hole (35) and is positioned on the mounting part (30), the right end of the active ingredient collecting part (3) is level with the right end of the mounting part (30), and the shaft hole (35) is used for being matched with the inserting port (10) of the injection and suction mechanism (1);
the right end of the active ingredient collecting part (3) is attached to the left end of the variable cavity (7);
a step (34) is arranged in the shell (2) and positioned at the left end of the variable chamber (7), and the active ingredient collecting part (3) is axially limited by means of the step (34);
the second channel (8) is provided with a vertical section and a horizontal section, the vertical section is perpendicular to the shaft hole (35), the horizontal section is parallel to the shaft hole (35), the exhaust valve (13) is arranged at the joint part of the vertical section and the horizontal section, and when the exhaust valve (13) is opened, the horizontal section and the valve hole of the exhaust valve (13) are used as exhaust channels; the included angle between the vertical section and the inclined section is 85 degrees.
2. The active ingredient enrichment device according to claim 1, wherein: the left end of the cylinder body (31) is provided with a sealing element, and the connecting part of the active ingredient collecting part (3) and the mounting part (30) is sealed by adopting the sealing element.
3. An enrichment method using the active ingredient enrichment device according to any of claims 1-2, wherein the steps comprise:
step 1, pulling a second piston assembly (11) of the injection mechanism (1) backwards to pump bone marrow blood into a containing cavity of the injection mechanism (1);
step 2, connecting the injection and suction mechanism (1) on the shell (2);
step 3, firstly opening an exhaust valve (13), then pushing a second piston assembly (11) of the injection and suction mechanism (1) forwards to enable bone marrow blood to enter a variable chamber (7) after passing through an active ingredient collecting part (3), in the process, the piston (4) moves rightwards, the volume of the variable chamber (7) is enlarged, and then closing the exhaust valve (13);
step 4, pulling the second piston assembly (11) of the injection mechanism (1) backwards to enable bone marrow blood in the variable chamber (7) to flow back into the accommodating cavity of the injection mechanism (1) through the second channel (8);
and 5, repeatedly pushing and pulling the second piston assembly (11) until the enrichment filling material (33) with the active ingredients is taken out after the enrichment is completed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310926281.2A CN116948783B (en) | 2023-07-26 | 2023-07-26 | Device and method for enriching active ingredients in bone marrow blood |
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| CN202310926281.2A CN116948783B (en) | 2023-07-26 | 2023-07-26 | Device and method for enriching active ingredients in bone marrow blood |
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| CN116948783A CN116948783A (en) | 2023-10-27 |
| CN116948783B true CN116948783B (en) | 2024-02-23 |
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| EP1093767A1 (en) * | 1999-10-21 | 2001-04-25 | Arthur Ashman | Combination syringe and aspirator for bone regeneration material and method for using the improved syringe |
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