CN116942736B - Compound preparation for invigorating stomach and promoting growth of ruminant animals, and preparation method and application thereof - Google Patents

Compound preparation for invigorating stomach and promoting growth of ruminant animals, and preparation method and application thereof Download PDF

Info

Publication number
CN116942736B
CN116942736B CN202310872257.5A CN202310872257A CN116942736B CN 116942736 B CN116942736 B CN 116942736B CN 202310872257 A CN202310872257 A CN 202310872257A CN 116942736 B CN116942736 B CN 116942736B
Authority
CN
China
Prior art keywords
parts
traditional chinese
chinese medicine
preparation
bacillus subtilis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202310872257.5A
Other languages
Chinese (zh)
Other versions
CN116942736A (en
Inventor
王德云
于亚明
武志勇
朱少武
李奎
施宗傲
刘振广
常广军
杨阳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Agricultural University
Original Assignee
Nanjing Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Agricultural University filed Critical Nanjing Agricultural University
Priority to CN202310872257.5A priority Critical patent/CN116942736B/en
Publication of CN116942736A publication Critical patent/CN116942736A/en
Application granted granted Critical
Publication of CN116942736B publication Critical patent/CN116942736B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/062—Ascomycota
    • A61K36/064—Saccharomycetales, e.g. baker's yeast
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/57—Magnoliaceae (Magnolia family)
    • A61K36/575—Magnolia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
    • A61K36/725—Ziziphus, e.g. jujube
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention belongs to the technical field of traditional Chinese veterinary medicines, and particularly discloses a compound preparation for invigorating stomach and promoting growth of ruminants, and a preparation method and application thereof, wherein the compound preparation comprises the following components: rhizoma Atractylodis, radix astragali, cortex Magnolia officinalis, and fructus Jujubae. The invention screens four traditional Chinese medicines of Chinese atractylodes, astragalus, magnolia bark and jujube for eliminating dampness and invigorating stomach, regulating stomach and nourishing spleen, researches the prevention and treatment effects of compatibility on food retention, rumen retardation and rumen acidosis of ruminants, determines the compatibility proportion of each traditional Chinese medicine, and has more advantages than a traditional Chinese medicine prescription for simply using stomach and digestion. The traditional Chinese medicine compound prepared by the formula can effectively reduce and control diseases such as rumen acidosis, rumen retardation and the like, and simultaneously achieves the effect of promoting growth.

Description

Compound preparation for invigorating stomach and promoting growth of ruminant animals, and preparation method and application thereof
Technical Field
The invention relates to the technical field of traditional Chinese veterinary medicines, in particular to a compound preparation for invigorating stomach and promoting growth of ruminants, and a preparation method and application thereof.
Background
Since ruminants have a special digestive system, the high coarse fiber forage grass is mainly used as a main food to ensure their health and growth. In the past decades, the farming industry has sought high production efficiency, often by supplementing large amounts of concentrate and using sub-therapeutic doses of antibiotics as feed additives, to increase the growth rate of ruminants and to increase feed conversion efficiency. However, the highly refined and concentrated feed such as concentrate is excessively supplemented, so that normal rumen fermentation and digestion processes of ruminants are often influenced, a series of problems such as nutrient waste, dyspepsia and rumen acidosis are caused, and the growth and health of ruminants are threatened. In addition, the long-term use of antibiotics not only can destroy rumen microbial flora of ruminants, but also can cause serious problems of antibiotic residues, bacterial drug resistance, environmental pollution and the like, thereby greatly threatening the health of animals and human beings. Thus, the search for a natural, green, safe feed additive to improve ruminant gastrointestinal function, and a novel feed additive that exerts a growth-promoting effect is a current problem that is in need of solution.
The traditional veterinary medicine is a traditional veterinary medicine in China, has the advantages of nature, safety, environmental protection, multiple effects, multiple action targets and the like, and is considered as the primary choice of the growth-promoting feed additive. A large number of researches prove that the veterinary drug can be used as an important nutrition source in ruminant feed, and has the health care and disease prevention effects. The application of the traditional Chinese veterinary medicine in ruminant feed is a research work with very wide application prospect and profound significance. Through robust and feasible research and application, the ruminant breeding efficiency and quality are hopeful to be improved, and contribution is made to the high-quality sustainable development of the animal husbandry.
Disclosure of Invention
The invention aims to: the invention aims to solve the technical problem of providing a compound preparation for invigorating stomach and promoting growth of ruminants, aiming at the defects of the prior art.
The invention also solves the technical problem of providing the preparation method of the compound preparation so as to solve the problems of simple crushing or simple decoction of the traditional Chinese medicine compound adopted by the traditional Chinese medicine commonly used in the livestock industry, simple preparation aspect and low oral availability.
The invention also solves the technical problem of providing the application of the compound preparation.
The invention is characterized in that: at present, the ruminant animal breeding industry is relatively extensive, and particularly, the breeding level of beef cattle and mutton sheep is lower than that of pigs and chickens, and a plurality of problems exist in aspects of nutrition, management and epidemic prevention and control, so that the breeding efficiency is generally low. The feed for ruminant is characterized by that it is mainly made up by using forage grass, and after the treatment of rumen fermentation, the forage grass can be fed into the real stomach and intestinal tract to make further absorption. But currently concentrate and other nutritional elements are additionally supplemented in order to increase the growth rate. High protein feeds can affect ruminant rumen fermentation, leading to dyspepsia. In addition, middle veterinarians consider that cows are of a damp-heat type and feeding a large amount of concentrated feed easily causes damp obstruction of middle energizer. Therefore, the method of eliminating dampness, strengthening spleen, promoting qi circulation, resolving dampness and removing food retention is needed to promote rumen fermentation and improve the digestion, absorption and utilization rate of the feed. The Chinese herbal medicine is taken as natural plants, and a plurality of medicinal materials have the characteristic of homology of medicine and food, so that the Chinese herbal medicine is taken as forage grass to be eaten by cattle and sheep when the cattle and sheep are raised by human grazing for thousands of years. On one hand, the Chinese medicinal composition can be used as a nutrient substance for digestion and absorption, and on the other hand, the Chinese medicinal active ingredients have a health care effect. At present, traditional Chinese medicines and preparations thereof are increasingly focused on the advantages of pure nature, no residue, no drug resistance and the like. Therefore, research and development of the traditional Chinese medicine compound which can strengthen stomach, promote nutrient digestion and absorption and promote growth of ruminants are of great significance. According to the theory and clinical experience of traditional Chinese medicine, the invention screens the following traditional Chinese medicines for prescription.
In order to solve the first technical problem, the invention discloses a compound preparation for invigorating stomach and promoting growth of ruminants, which is prepared from the following components: rhizoma Atractylodis, radix astragali, cortex Magnolia officinalis, and fructus Jujubae.
Rhizoma atractylodis: warm nature, pungent and bitter taste, enter spleen and stomach meridians. Has effects of eliminating dampness, invigorating spleen, dispelling pathogenic wind and dispelling cold. It is often used for treating spleen-stomach obstruction, spleen dysfunction in transportation, and symptoms such as gastric and abdominal distention, inappetence, listlessness and debilitation.
Radix astragali: slightly warm nature, sweet taste, entering the lung and spleen meridians. Has effects of invigorating qi, raising yang, inducing diuresis for removing edema, etc., and is commonly used for qi deficiency of spleen and stomach and sinking of middle-jiao. Modern medical research shows that astragalus root also has the functions of resisting oxidation, improving immunity and strengthening heart and promoting urination.
Cortex Magnoliae officinalis: warm nature, bitter and pungent taste, enter spleen, stomach, lung and large intestine meridians. Has effects of eliminating dampness and phlegm, descending qi and eliminating fullness. It is often indicated for damp-stagnation, vomiting and diarrhea due to distention and fullness, food stagnation, abdominal distention, constipation, phlegm-fluid retention, dyspnea and cough. The active ingredients of magnolia bark, such as magnolol, in modern medical research have excitation effect on intestinal tracts.
Jujube: has warm nature, sweet taste, spleen and stomach channel entering, spleen invigorating, qi invigorating, blood nourishing, tranquillizing, etc., and can be used for treating spleen deficiency, anorexia, loose stool, listlessness, debilitation, contains protein, saccharide, organic acid, mucilage, vitamins, microelements, various amino acids, etc. Has effects in protecting liver, and enhancing muscle strength and body weight.
The principal drug in the formula provided by the invention is rhizoma atractylodis (strengthening spleen and eliminating dampness, dispelling wind and dispelling cold), the ministerial drug is radix astragali (tonifying qi and raising yang, inducing diuresis and detumescence, resisting oxidation and regulating immunity), the adjuvant drug is cortex magnoliae officinalis (relaxing bowels and promoting qi circulation, descending qi and removing fullness, promoting digestion), and the Chinese date is the guiding drug (tonifying middle-jiao and replenishing qi, nourishing blood and tranquillizing, and promoting growth). The formula is used for differentiating the special physique and symptoms of rumen retardation of ruminants according to the middle veterinarian syndrome differentiation theory, and adopts the principle formula of drying dampness and strengthening spleen, promoting qi and regulating middle warmer, tonifying qi and raising yang, and tonifying qi and nourishing blood, so that the digestion function of ruminants is promoted, the immunity is improved, the gastrointestinal health is regulated, and good growth promoting and antioxidation effects are achieved.
Wherein the weight portions of the components are as follows: 30-40 parts of rhizoma atractylodis, 30-40 parts of astragalus membranaceus, 10-20 parts of magnolia officinalis and 10-20 parts of Chinese date, preferably 33-37 parts of rhizoma atractylodis, 33-37 parts of astragalus membranaceus, 13-17 parts of magnolia officinalis and 13-17 parts of Chinese date, and further preferably 35 parts of rhizoma atractylodis, 35 parts of astragalus membranaceus, 15 parts of magnolia officinalis and 15 parts of Chinese date.
In order to solve the second technical problem, the invention discloses a preparation method of the compound preparation, which comprises the following steps:
s1: weighing the components according to the formula ratio, and crushing for later use;
s2: adding auxiliary materials into the crushed materials obtained in the step S1, and mixing;
s3: and (3) inoculating the strain into the mixture obtained in the step (S2), and fermenting to obtain the compound preparation.
In the step S1, the crushing is carried out to 60-80 meshes, and the fineness of the powder is suitable for fermentation.
In the step S2, the auxiliary materials are soybean meal, corn flour, molasses and K 2 PO 4 Any one or more of the compositions, preferably soybean meal, corn meal, molasses and K 2 PO 4 Further preferred are soybean meal, corn meal, molasses and K 2 PO 4 The addition amounts of (a) are respectively 1% -3%, 0.5% -1.5% and 0.1% -1% of the total mass of the components in the step S1, preferably 2%, 1% and 0.5%.
In the step S3, the obtained mixture is added with water to adjust the water content in the mixture to be 35-40 wt%.
In the step S3, the strains are any one or a combination of a plurality of bacillus subtilis, saccharomyces cerevisiae and lactobacillus, preferably the mixture of the bacillus subtilis, the saccharomyces cerevisiae and the lactobacillus after screening; preferably, the bacillus subtilis, the saccharomyces cerevisiae and the lactobacillus are bacillus subtilis CGMCC1.15792, saccharomyces cerevisiae CGMCC2.3973 and lactococcus lactis CGMCC1.15072 respectively.
In the step S3, the strain is inoculated into the mixture obtained in the step S2 according to 1% -5% of the total mass of the components in the step S1; preferably 3%; preferably, the access mass ratio of the bacillus subtilis to the saccharomyces cerevisiae to the lactobacillus is 1-3:0.5-1.5:1, and preferably 2:1:1.
In the step S3, the concentration of the strain in the bacterial liquid is 1-3 multiplied by 10 5 CFU/ml, preferably 1.8X10 5 CFU/ml; preferably, the temperature of the fermentation is 35-37 ℃; preferably, the fermentation time of the fermentation is 5-9 days.
In order to solve the third technical problem, the invention discloses application of the compound preparation in preparing a stomach-invigorating, growth-promoting or antioxidant additive or feed for ruminants.
Wherein the ruminant is a cow or sheep.
Wherein, when the ruminant is a cow, the dosage of the compound preparation is 0.25-1.0 g of the traditional Chinese medicine preparation per kg of body weight per day, preferably 0.5g of the traditional Chinese medicine preparation per kg of body weight per day; when the ruminant is sheep, the dosage of the compound preparation is 0.25-0.75 g of traditional Chinese medicine preparation/kg of weight/day, preferably 0.5g of traditional Chinese medicine preparation/kg of weight/day.
The beneficial effects are that: compared with the prior art, the invention has the following advantages:
(1) The invention screens four traditional Chinese medicines of Chinese atractylodes, astragalus, magnolia bark and jujube for eliminating dampness and invigorating stomach, regulating stomach and nourishing spleen, researches the prevention and treatment effects of compatibility on food retention, rumen retardation and rumen acidosis of ruminants, determines the compatibility proportion of each traditional Chinese medicine, and has more advantages than a traditional Chinese medicine prescription for simply using stomach and digestion. The traditional Chinese medicine compound prepared by the formula can effectively reduce and control diseases such as rumen acidosis, rumen retardation and the like, and simultaneously achieves the effects of promoting growth and resisting oxidation.
(2) The fermentation process is researched, a proper fermentation method is established, a good wall breaking effect is achieved, and active ingredients are easier to absorb.
(3) The invention performs synergistic action on the product preparation through optimizing strains, microbial fermentation products and traditional Chinese medicine components, can effectively promote the rumen digestion function, promote nutrient absorption, and achieve the effects of promoting growth and resisting oxidation.
Drawings
The foregoing and/or other advantages of the invention will become more apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings and detailed description.
Fig. 1 is a beef cattle test.
Figure 2 is a yak test.
Fig. 3 is a mutton sheep test.
Detailed Description
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, are commercially available.
In the following examples, the bacterial liquid is prepared by mixing the bacterial strains of bacillus subtilis, saccharomyces cerevisiae and lactobacillus in a ratio of 2:1:1, the preparation method adopts dry bacterial powder, the dry bacterial powder is added into sterile purified water according to the calculated proportion of the detection content, and the concentration is diluted to 1.8x10 bacterial concentration 5 CFU/ml。
In the following examples, the adjuvants including soybean meal, corn meal, molasses and K 2 PO 4 The percentage of (2) is mass percentage.
Example 1: preparation of Chinese medicine compound preparation
The formula comprises the following components: 35 parts of rhizoma atractylodis, 35 parts of astragalus mongholicus, 15 parts of magnolia officinalis and 15 parts of Chinese date.
The preparation method comprises the following steps:
(1) Screening rhizoma atractylodis, astragalus membranaceus, magnolia officinalis and Chinese date, removing impurities, weighing the above medicinal materials according to the weight part ratio of the formula, and crushing the medicinal materials to 60-80 meshes for later use;
(2) Adding soybean meal, corn flour, molasses and K into the material obtained in the step (1) 2 PO 4 The addition ratio is 2%, 1% and 0.5% of the mass ratio of the materials obtained in the step (1), and the concentration of the added strain is 1.8X10 5 The mixed bacterial liquid prepared by bacillus subtilis CGMCC1.15792, saccharomyces cerevisiae CGMCC2.3973 and lactobacillus CGMCC1.15072 with the CFU/ml is added with 3 percent of the mass ratio of the materials obtained in the step (1), and then water is added to adjust the water content of the materials to be 35-40 percent, the fermentation temperature is 35-37 ℃ and the fermentation time is 7 days, so that the traditional Chinese medicine compound preparation is prepared.
Example 2: test result of traditional Chinese medicine compound preparation for beef cattle growth promotion
45 Siemens beef cattle are selected for experiment, and randomly divided into 3 groups, namely a control group, a traditional Chinese medicine low-dose group and a traditional Chinese medicine high-dose group, wherein each group is repeated by 3 times, and each time is repeated by 5 cattle. The traditional Chinese medicine compound is added into the low-dose group beef cattle according to 0.25g of traditional Chinese medicine preparation/kg of weight/day in the basic ration, the traditional Chinese medicine compound is added into the medium-dose group beef cattle according to 0.5g of traditional Chinese medicine preparation/kg of weight/day in the basic ration, the traditional Chinese medicine compound is added into the high-dose group beef cattle according to 1.0g of traditional Chinese medicine preparation/kg of weight/day in the basic ration, and the traditional Chinese medicine compound is not added into the control group. The test period was 66d. The ear marks of each beef cattle were recorded before the start of the test and all beef cattle weights were weighed and recorded as initial weights, the final weights of all beef cattle were weighed corresponding to the ear marks after 66d of fattening, recorded as final weights, and the average daily gain of each group of beef cattle was calculated according to the initial weights and the final weights. Average daily gain= (last-first weight)/test day. In addition, the total feed intake of each group of beef cattle is recorded, and the average daily feed intake is calculated. Average daily feed intake = total feed intake/number of days tested/head. Calculating feed conversion rate and feed meat ratio according to average daily gain and average daily feed intake, wherein feed meat ratio=average daily feed intake/average daily gain; feed conversion = daily gain/daily intake x 100%. Data are expressed as Mean ± Standard Error (SEM), and were statistically analyzed using SPSS software. The data were analyzed for significance using one-way analysis of variance. P values less than 0.05 were considered significant differences. The test results are as follows (table 1):
table 1 influence of the Chinese herbal Compound on the growth Properties of beef cattle
Note that: * P <0.05, < P <0.01 (comparison of control group and traditional Chinese medicine group)
As shown in fig. 1 and table 1, the test results showed that: compared with the control group, the beef cattle powder weights of the traditional Chinese medicine low-dose group, the middle-dose group and the high-dose group have no significant difference (P > 0.05); however, the average daily gain of beef cattle in the traditional Chinese medicine medium-dose group and the traditional Chinese medicine high-dose group is obviously higher than that in a control group (P < 0.01), and further the feed conversion rate and the feed conversion rate of beef cattle in the traditional Chinese medicine low-dose group, the traditional Chinese medicine medium-dose group and the traditional Chinese medicine high-dose group are obviously higher than those in the control group, the feed conversion rate of beef cattle in the traditional Chinese medicine low-dose group and the traditional Chinese medicine high-dose group is obviously lower than those in the control group (P < 0.01), and the results indicate that the traditional Chinese medicine compound can obviously reduce the feed conversion rate, improve the growth performance of beef cattle and the effect of the traditional Chinese medicine compound medium-dose group is most obvious.
Example 3: test result of traditional Chinese medicine compound preparation for promoting growth of yaks
83 yaks 3-4 years old were selected for the trial and randomly divided into a control group (n=48) and a traditional Chinese medicine group (n=35). The traditional Chinese medicine compound prepared in the example 1 is added into the basic ration of the traditional Chinese medicine group yaks, the traditional Chinese medicine compound is added according to the dosage of 0.5g of traditional Chinese medicine preparation/kg of weight/day, the traditional Chinese medicine compound is continuously fed for 32 days, then the traditional Chinese medicine compound is not added into the basic ration of the traditional Chinese medicine group yaks, and then the fattening is carried out for 53 days for total fattening for 85 days; the basic daily ration is not added into the basic daily ration of the yaks in the control group, and the total fattening is carried out for 99 days. The yaks were free to ingest and drink water throughout the test. Before the start of the experiment, each yak body weight was weighed and recorded as initial weight. After fattening, the weight of each yak is weighed and recorded as the last weight. Average daily gain was calculated from the last and initial weight of each yak. Average daily gain of yaks = (last-first weight)/days of fattening. Data are expressed as Mean ± Standard Error (SEM), and were statistically analyzed using SPSS software. The data were analyzed for significance using one-way analysis of variance. P values less than 0.05 were considered significant differences. The test results are as follows (table 2):
table 2 influence of the herbal compound on the growth performance of yaks
Note that: * P<0.05， ** P<0.01 (comparison of control group with traditional Chinese medicine group)
As shown in fig. 2 and table 2, the test results showed that: by adding the traditional Chinese medicine compound into the basic ration of the yaks, the average daily gain of the yaks can be obviously improved, and compared with a control group, the final weight of the yaks in the traditional Chinese medicine group is obviously increased (P is less than 0.01); meanwhile, the average daily gain of the yaks in the traditional Chinese medicine group is obviously higher than that in the control group (P < 0.01), and the results show that the traditional Chinese medicine compound has obvious growth promoting effect.
Example 4: experimental result of Chinese herbal compound preparation for promoting growth of mutton sheep
64 weaned lambs with equivalent body conditions (18.79+/-0.32 kg) are selected in the experiment, and after the weaned lambs are adaptively fed for one week, the weaned lambs are randomly divided into 4 groups, namely a control group, a traditional Chinese medicine low-dose group, a traditional Chinese medicine medium-dose group and a traditional Chinese medicine high-dose group, wherein each group is divided into 16 replicates, and each replicate is 1. The control group is fed with basic ration, and the traditional Chinese medicine group is added with the traditional Chinese medicine compound prepared in the embodiment 1 with different dosages in the basic ration, namely, the traditional Chinese medicine low-dosage group is added with the traditional Chinese medicine compound according to 0.25g of traditional Chinese medicine preparation/kg of weight/day, the traditional Chinese medicine medium-dosage group is added with the traditional Chinese medicine compound according to 0.5g of traditional Chinese medicine preparation/kg of weight/day, and the traditional Chinese medicine high-dosage group is added with the traditional Chinese medicine compound according to 0.75g of traditional Chinese medicine preparation/kg of weight/day. During the pre-test period for 7 days, all the Hu sheep are fed with basic daily ration, wherein the basic daily ration consists of 30% of peanut seedlings, 20% of corn silage, 20% of bean curd residues and 30% of special concentrate for fattening the Hu sheep, and the specific ingredients of the concentrate are shown in table 3. After feeding for 7 days in the pre-test period, adding Chinese herbal compound with different dosages into the basic ration of the Chinese herbal group, and feeding for 39 days. Feeding is carried out once every day at a ratio of 7:30 and 16:30, and all the Hu sheep eat and drink water freely during the whole experimental process.
After 7 days of pre-test feeding, the body weight of all the Hu sheep was weighed on an empty stomach and recorded as initial weight. After the experiment, the body weight of all the Hu sheep was weighed on an empty stomach, recorded as the final weight, and the average daily gain was calculated. Average daily gain= (last-first weight)/test day. The feeding amount and the residual amount are recorded every day, the residual amount is ensured to be about 10% of the feeding amount, the feeding amount is adjusted at any time according to the proportion of the residual amount to the feeding amount on the same day, the residual amount is weighed and recorded before feeding every morning, and the average daily feed intake is calculated. Average daily feed intake= (feed amount-residual feed amount)/day. Calculating feed conversion rate and feed conversion rate according to average daily feed intake and average daily weight gain, namely feed conversion rate = daily feed intake/daily weight gain; feed conversion = daily gain/daily intake x 100%. On experiment day 39, collecting gastric juice of each group of Hu sheep rumen by using a rumen liquid collecting tube in a sterile centrifuge tube, and detecting PH value change of the rumen contents of the Hu sheep by using a portable PH meter; and meanwhile, sheep blood is collected to separate serum and is used for detecting related indexes of the antioxidant capacity of the serum. Data obtained from the experiments are expressed in mean±sem and statistically analyzed using SPSS software (SPSS 26.0, ibm corporation). The data were analyzed for significance using one-way analysis of variance. P values less than 0.05 were considered significant differences. The specific test results are as follows (tables 3-6):
table 3 concentrate formula special for fattening Hu sheep
Note that: [1] the premix comprises Fe, cu, zn, mn, se, I, co, VA, VD and VE.
TABLE 4 influence of Chinese herbal compound on growth performance of Hu sheep
Note that: * P <0.05, < P <0.01 (comparison of control group and traditional Chinese medicine group)
TABLE 5 influence of Chinese herbal Compound on gastric juice PH of Hu sheep tumor
TABLE 6 influence of Chinese herbal compound on antioxidant capacity of Hu sheep
Note that: * P <0.05, < P <0.01 (comparison of control group and traditional Chinese medicine group)
As shown in fig. 3 and tables 4-6, the test results showed that: the effect of the traditional Chinese medicine formulation on the weight and feed intake of Hu sheep is shown in table 4. The initial weights of the Hu sheep in each group were not significantly different, and the weight of the Hu sheep powder in all traditional Chinese medicine groups was higher than that of the control group, although the difference was not significant in statistics (P > 0.05); in addition, the average daily gain of the Hu sheep in each traditional Chinese medicine group is higher than that in a control group, and the average daily gain of the Hu sheep in the traditional Chinese medicine group at the dosage is 161.22g; average daily feed intake of the Hu sheep in each group has no significant change; the meat ratio of the traditional Chinese medicine group lake sheep is lower than that of the control group, the difference is extremely obvious (P is less than 0.01), and the meat ratio of the traditional Chinese medicine group sheep is the lowest in dosage; the feed conversion rate of the Hu sheep in the traditional Chinese medicine group is obviously higher than that of the control group (P < 0.01), and the feed conversion rate is highest in the traditional Chinese medicine group at the dosage.
The pH of gastric juice of each group of Hu sheep tumor is shown in Table 5. The normal PH range of the Hu sheep rumen fluid is 5.5-7.5, the PH of the Hu sheep rumen fluid in the control group and the traditional Chinese medicine groups is in the normal range, and the PH of the Hu sheep rumen fluid in the traditional Chinese medicine groups is not obviously different from that in the control group (P is more than 0.05), which indicates that the traditional Chinese medicine formula does not influence the PH of the Hu sheep rumen fluid.
The superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) of the serum of each group of Hu sheep are shown in Table 6. Compared with the control group, the SOD activity of sheep serum in the low, medium and high dose groups of the traditional Chinese medicine is obviously higher than that in the control group (P < 0.05); the sheep serum T-AOC activity of the low, medium and high dose groups of the traditional Chinese medicine is significantly higher than that of the control group (P <0.05 or P < 0.01). The sheep serum MDA levels of the low, medium and high dose groups of the traditional Chinese medicine are significantly lower than those of the control group (P <0.05 or P < 0.01). The results suggest that the traditional Chinese medicine compound can improve the oxidation resistance of the Hu sheep.
In conclusion, the traditional Chinese medicine compound can obviously improve the feed conversion rate of the Hu sheep, reduce the feed-meat ratio, improve the oxidation resistance of organisms, and can not damage the balance of the rumen PH of the Hu sheep, so that the traditional Chinese medicine compound has the effect of improving the growth performance of the Hu sheep.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims (14)

1. The compound preparation for promoting the growth of ruminants is characterized by being prepared from the following components in parts by weight: 30-40 parts of rhizoma atractylodis, 30-40 parts of astragalus mongholicus, 10-20 parts of magnolia officinalis and 10-20 parts of jujube; the ruminant is a bovine;
the preparation method of the compound preparation comprises the following steps:
s1: weighing the components according to the formula ratio, and crushing for later use;
s2: adding auxiliary materials into the crushed materials obtained in the step S1, and mixing;
s3: inoculating strains into the mixture obtained in the step S2, and fermenting to obtain the compound preparation;
in the step S2, the auxiliary materials are soybean meal, corn flour, molasses and K 2 PO 4 Is a combination of (a);
in the step S3, the strain is a mixture of bacillus subtilis, saccharomyces cerevisiae and lactobacillus; the bacillus subtilis, the saccharomyces cerevisiae and the lactobacillus are respectively bacillus subtilis CGMCC1.15792, saccharomyces cerevisiae CGMCC2.3973 and lactococcus lactis CGMCC1.15072; the access mass ratio of the bacillus subtilis to the saccharomyces cerevisiae to the lactobacillus is 1-3:0.5-1.5:1.
2. The compound preparation according to claim 1, wherein the components are as follows in parts by weight: 33-37 parts of rhizoma atractylodis, 33-37 parts of astragalus membranaceus, 13-17 parts of magnolia officinalis and 13-17 parts of jujube.
3. The compound preparation according to claim 1, wherein the components are as follows in parts by weight: 35 parts of rhizoma atractylodis, 35 parts of astragalus mongholicus, 15 parts of magnolia officinalis and 15 parts of Chinese date.
4. A method for preparing a compound preparation according to any one of claims 1 to 3, comprising the steps of:
s1: weighing the components according to the formula ratio, and crushing for later use;
s2: adding auxiliary materials into the crushed materials obtained in the step S1, and mixing;
s3: inoculating strains into the mixture obtained in the step S2, and fermenting to obtain the compound preparation;
in the step S2, the auxiliary materials are soybean meal, corn flour, molasses and K 2 PO 4 Is a combination of (a);
in the step S3, the strain is a mixture of bacillus subtilis, saccharomyces cerevisiae and lactobacillus; the bacillus subtilis, the saccharomyces cerevisiae and the lactobacillus are respectively bacillus subtilis CGMCC1.15792, saccharomyces cerevisiae CGMCC2.3973 and lactococcus lactis CGMCC1.15072; the access mass ratio of the bacillus subtilis to the saccharomyces cerevisiae to the lactobacillus is 1-3:0.5-1.5:1.
5. The method according to claim 4, wherein in the step S1, the pulverization is carried out to 60 to 80 mesh.
6. The process according to claim 4, wherein the soybean meal, corn meal, molasses and K 2 PO 4 The addition amounts of the components are respectively 1% -3%, 0.5% -1.5% and 0.1% -1% of the total mass of the components in the step S1.
7. The process according to claim 4, wherein in step S3, the mixture is adjusted by adding water to control the water content in the mixture to be 35 to 40wt%.
8. The method according to claim 4, wherein in step S3, the strain is introduced into the mixture obtained in step S2 in an amount of 1% -5% of the total mass of the components described in step S1.
9. The preparation method according to claim 4, wherein the access mass ratio of the bacillus subtilis, the saccharomyces cerevisiae and the lactobacillus is 2:1:1.
10. The method according to claim 4, wherein in step S3, the concentration of the strain in the bacterial liquid is 1-3×10 5 CFU/ml。
11. The process according to claim 4, wherein,in the step S3, the concentration of the strain in the bacterial liquid is 1.8x10 5 CFU/ml。
12. The process according to claim 4, wherein in step S3, the fermentation temperature is 35-37 ℃.
13. The method according to claim 4, wherein the fermentation time of the fermentation in step S3 is 5 to 9 days.
14. Use of a compound formulation according to any one of claims 1-3 in the preparation of a growth-promoting or antioxidant additive or feed for ruminants, which ruminants are cattle.
CN202310872257.5A 2023-07-17 2023-07-17 Compound preparation for invigorating stomach and promoting growth of ruminant animals, and preparation method and application thereof Active CN116942736B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310872257.5A CN116942736B (en) 2023-07-17 2023-07-17 Compound preparation for invigorating stomach and promoting growth of ruminant animals, and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310872257.5A CN116942736B (en) 2023-07-17 2023-07-17 Compound preparation for invigorating stomach and promoting growth of ruminant animals, and preparation method and application thereof

Publications (2)

Publication Number Publication Date
CN116942736A CN116942736A (en) 2023-10-27
CN116942736B true CN116942736B (en) 2024-03-29

Family

ID=88442107

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310872257.5A Active CN116942736B (en) 2023-07-17 2023-07-17 Compound preparation for invigorating stomach and promoting growth of ruminant animals, and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN116942736B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101623055A (en) * 2009-07-31 2010-01-13 山西天沐合生农牧科技开发有限公司 Chinese herbal medicine feed additive for lambs
CN102232998A (en) * 2011-04-15 2011-11-09 北京康华远景科技有限公司 Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof
CN105767543A (en) * 2016-02-29 2016-07-20 河南牧业经济学院 Stomach-strengthening and digestion-promoting traditional Chinese medicine micro-ecological preparation for piglet and preparation method thereof
CN107691835A (en) * 2017-10-29 2018-02-16 西安乐民反刍动物研究所 A kind of dregs of a decoction fermentation prepares the method that ruminant mixes daily ration entirely

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101623055A (en) * 2009-07-31 2010-01-13 山西天沐合生农牧科技开发有限公司 Chinese herbal medicine feed additive for lambs
CN102232998A (en) * 2011-04-15 2011-11-09 北京康华远景科技有限公司 Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof
CN105767543A (en) * 2016-02-29 2016-07-20 河南牧业经济学院 Stomach-strengthening and digestion-promoting traditional Chinese medicine micro-ecological preparation for piglet and preparation method thereof
CN107691835A (en) * 2017-10-29 2018-02-16 西安乐民反刍动物研究所 A kind of dregs of a decoction fermentation prepares the method that ruminant mixes daily ration entirely

Also Published As

Publication number Publication date
CN116942736A (en) 2023-10-27

Similar Documents

Publication Publication Date Title
CN102232487B (en) Physiological regulator for maternal animals, and preparation method thereof
WO2017219498A1 (en) Fermented soybean meal and preparation method therefor
CN105053637A (en) Pig intestine membrane protein-containing pigling mixed feed and preparation method thereof
KR101776277B1 (en) A composition for animal feed additive and A composition for animal feed
CN106306512A (en) Fermented Chinese herbal medicine feed additive for treating porcine diarrhea
CN109221675A (en) A kind of feed additive for weaning piglet and preparation method thereof
CN106387435A (en) Feed additive, and application thereof, as well as feed
CN109350655A (en) A kind of fermented tcm and preparation method thereof improving pig growth and reproductive performance
CN109349434A (en) A kind of compound premix preventing ruminant tumor gastric acid poisoning
CN105432994A (en) Pig fodder capable of improving daily body weight gain rate of pig and preparation method of pig fodder
CN112426501B (en) Traditional Chinese medicine microecological preparation for promoting growth and preventing diarrhea of calves and preparation method thereof
CN107095061A (en) Improve the fermented feed preparation method and applications of growing and fattening pigs gut barrier function
CN106804916A (en) Boer goat pregnancy ewes complete feed and preparation method thereof
CN106260658A (en) One boar anti-heat stress Chinese herbal medicine feed additive
CN116942736B (en) Compound preparation for invigorating stomach and promoting growth of ruminant animals, and preparation method and application thereof
CN114176170B (en) Functional feed additive and preparation method and application thereof
CN110089619A (en) A kind of Chinese medicine bacteria preparation fermented feed and preparation method thereof
CN110074274A (en) A kind of growth pannage and preparation method thereof
CN108850613A (en) Fattening pannage and preparation method thereof
CN107595988A (en) It is a kind of to be used to prevent and treat enteric modulators of prevention of sow constipation and preparation method thereof
CN106798111A (en) Boer goat wet sheep complete feed and preparation method thereof
CN106333068A (en) Piglet feed
CN110506854A (en) A kind of cattle and sheep health-caring feedstuff addictive and the preparation method and application thereof
CN109221696A (en) Sow probiotic feed and preparation method thereof
CN106721463A (en) Chinese herb feed additive for broiler chicken and preparation method and application

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant