CN116941719A - Hericium erinaceus polysaccharide small peptide probiotic solid beverage and preparation method thereof - Google Patents
Hericium erinaceus polysaccharide small peptide probiotic solid beverage and preparation method thereof Download PDFInfo
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- CN116941719A CN116941719A CN202310958832.3A CN202310958832A CN116941719A CN 116941719 A CN116941719 A CN 116941719A CN 202310958832 A CN202310958832 A CN 202310958832A CN 116941719 A CN116941719 A CN 116941719A
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- Prior art keywords
- hericium erinaceus
- small peptide
- solid beverage
- erinaceus polysaccharide
- powder
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Abstract
The invention provides a hericium erinaceus polysaccharide small peptide probiotic solid beverage and a preparation method thereof, and relates to the technical field of food processing. The hericium erinaceus polysaccharide small peptide probiotic solid beverage is composed of hericium erinaceus polysaccharide extract, cocoa small peptide extract, probiotics, glucose oxidase and other raw materials. The hericium erinaceus polysaccharide small peptide probiotic solid drink has good human body absorption and utilization rate, good immunoregulation function, and can slow down the metabolism rate of alcohol in a human body and the concentration of alcohol in blood, so that the harm of alcohol to the human body is reduced. The hericium erinaceus polysaccharide small peptide probiotic solid beverage has good stability, good sensory quality and flavor after being brewed, and can meet the preference of most consumers.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a hericium erinaceus polysaccharide small peptide probiotic solid beverage and a preparation method thereof.
Background
Hericium erinaceus, which is known as Hericium erinaceus, is an edible fungus and also a medicinal fungus. Hericium erinaceus is one of the eight major "delicacies" in China, and has the theory of "delicacies hericium erinaceus and sea-flavor bird's nest". Hericium erinaceus is tender in meat quality, delicious in taste and rich in nutrition, contains various active ingredients, and has certain health care effects on digestive tract, nervous system, immune system and the like.
Hericium erinaceus has high nutritive value, and contains abundant nutritional ingredients such as protein, vitamin B1, vitamin B2, vitamin B6, calcium, ferrum, phosphorus, and active substances such as unsaturated fatty acid, polysaccharide, and small peptides. The efficacy and effect of the hericium erinaceus are as follows: reducing cholesterol and triglyceride, regulating blood lipid, promoting blood circulation, and preventing cardiovascular diseases; enhancing immunity, improving resistance of human body to various viruses and bacteria, and preventing respiratory tract infection such as common cold and influenza; anti-cancer and anti-tumor, can inhibit the synthesis of genetic material in cancer cells, thereby preventing and treating digestive tract cancer and other malignant tumors; maintenance of intestines and stomach, has the functions of protecting, conditioning and repairing the digestive tract system, has multiple effects of helping digestion, tonifying liver and spleen, allaying hunger and quenching thirst, eliminating toxin, and the like, has remarkable curative effects on digestive tract diseases such as inappetence, nausea and vomiting, and can quickly arrest vomiting after drinking the product, and has remarkable curative effects on gastric ulcer, duodenal ulcer, gastritis, and the like; has anti-aging effect and special effect on neurasthenia and insomnia. Can promote the growth and regeneration of cerebral nerve cells, has good effect on preventing and treating Alzheimer's disease, and is a novel nutritional brain-strengthening food with life prolonging and anti-aging effects.
There are various types of hericium erinaceus products on the market at present, such as health-care drinks, biscuits, cans, teabags and the like. The products all utilize the nutritional and medicinal value of hericium erinaceus, such as polysaccharide, protein, small peptide, terpenes and the like. However, these products have low conversion rate and low content of active ingredients, cannot achieve the expected effect, have flavor and taste unsuitable for the preference of all people, and have simple composition. In addition, the existing hericium erinaceus products are mainly on the stomach nourishing effect, and other beneficial effects are not fully developed.
Disclosure of Invention
In order to overcome the problems of the existing hericium erinaceus products and enrich the product efficacy, the invention provides a hericium erinaceus polysaccharide small peptide probiotic solid beverage and a preparation method thereof. According to the invention, the hericium erinaceus mycelium, the cocoa beans and the probiotics are taken as raw materials, and the hericium erinaceus mycelium, the cocoa beans and the probiotics are extracted and compounded to obtain the solid beverage product with the immunoregulation function, which can promote alcohol metabolism and has good flavor and sensory quality, so that the form and the efficacy of the hericium erinaceus product are enriched.
The hericium erinaceus polysaccharide small peptide probiotics solid beverage disclosed by the invention comprises the following raw materials in parts by weight:
60-80 parts of hericium erinaceus polysaccharide extract, 30-50 parts of cocoa small peptide extract, 5-30 parts of probiotics and 1-5 parts of glucose oxidase.
Further, the hericium erinaceus polysaccharide extract is composed of hericium erinaceus fruiting body polysaccharide and hericium erinaceus mycelium polysaccharide according to a weight ratio of 2:1.
Further, the hericium erinaceus polysaccharide small peptide probiotics solid beverage also comprises 1-10 parts of sweetener, 1-5 parts of vitamin and 1-5 parts of mineral.
The hericium erinaceus polysaccharide extract is obtained by extracting hericium erinaceus mycelia. Hericium erinaceus polysaccharide is the main active substance in Hericium erinaceus, and has multiple biological functions. The structure of the hericium erinaceus polysaccharide mainly comprises beta-glucan as a main chain, and different branched chains are connected, wherein the branched chains contain monosaccharides such as glucose, rhamnose, arabinose, mannose, galactose and the like. The molecular weight and monosaccharide composition ratio of Hericium erinaceus polysaccharide may vary due to different extraction methods and sources.
In general, hericium erinaceus polysaccharide refers to polysaccharide extracted from fruiting bodies of Hericium erinaceus, and Hericium erinaceus mycelium polysaccharide refers to polysaccharide extracted from liquid submerged fermentation mycelium of Hericium erinaceus. The monosaccharide compositions of the Hericium erinaceus polysaccharide and the Hericium erinaceus mycelium polysaccharide are different, the Hericium erinaceus fruiting body polysaccharide mainly comprises fucose, glucose and galactose, and the mycelium polysaccharide comprises arabinose, xylose, galactose and glucose. In addition, the biological activities of Hericium erinaceus polysaccharide and Hericium erinaceus mycelium polysaccharide also have differences. In particular, the hericium erinaceus fruiting body polysaccharide has a particularly outstanding protective effect on gastric mucosa, and the mycelium polysaccharide has a better protective effect on liver. Therefore, the invention combines the Hericium erinaceus fruiting body polysaccharide and Hericium erinaceus mycelium polysaccharide to prepare the solid beverage product.
The preparation method of the hericium erinaceus fruiting body polysaccharide extract comprises the following steps:
adding 5-10 times of water into the hericium erinaceus fruiting body, performing ultrasonic extraction for 2-4 hours at the temperature of 70 ℃ under 20000-50000 HZ, filtering by using diatomite, concentrating under reduced pressure, and freeze-drying to obtain the hericium erinaceus fruiting body polysaccharide extract.
The preparation method of the hericium erinaceus mycelium polysaccharide extract comprises the following steps:
freeze-drying Hericium erinaceus mycelium, grinding into powder, sieving with 200 mesh sieve to obtain Hericium erinaceus mycelium freeze-dried powder, mixing the Hericium erinaceus mycelium freeze-dried powder with ethanol solution, extracting for the first time, centrifuging the extractive solution after the extraction is finished to obtain supernatant, extracting and purifying the supernatant with ethanol solution for the second time, centrifuging the extractive solution after the purification to obtain supernatant, concentrating the supernatant under reduced pressure, and freeze-drying to obtain Hericium erinaceus polysaccharide extract.
Further, the concentration of the ethanol solution is 50% -100%.
Preferably, the concentration of the ethanol solution is 70% -95%.
Further, the first extraction adopts an ultrasonic auxiliary extraction process, and the feed liquid ratio of the hericium erinaceus mycelium to the ethanol solution is 1g:10-30mL, ultrasonic frequency of 20-100KHz, and extraction time of 20-60min.
Preferably, the first extraction adopts an ultrasonic auxiliary extraction process, and the feed liquid ratio of the hericium erinaceus mycelium to the ethanol solution is 1g:25mL, wherein the ultrasonic frequency is 50-85KHz, and the extraction time is 30-40min.
Further, the secondary extraction and purification adopts an ultrasonic auxiliary extraction process, the volume ratio of the extracting solution to the ethanol solution is 1:5-10, the ultrasonic frequency is 20-100KHz, and the extraction time is 10-40min.
Preferably, the secondary extraction and purification adopts an ultrasonic auxiliary extraction process, the volume ratio of the extracting solution to the ethanol solution is 1:10, the ultrasonic frequency is 50-85KHz, and the extraction time is 15-30min.
The higher the ultrasonic frequency is, the higher the extraction efficiency is, the better the purification effect is, but the higher the ultrasonic frequency is, the thermal effect and the degradation effect exist, and the quality of the extract is affected. Therefore, the present invention limits the ultrasonic frequency to 20-100KHz so as to obtain high quality extract while ensuring extraction efficiency and purification effect.
Further, the centrifugal rotation degree is 500-6000r/min, and the centrifugal time is 10-15min.
The preparation method of the cocoa small peptide extract comprises the following steps:
mixing cocoa powder and chlorohydrin, extracting under reflux, centrifuging to obtain defatted cocoa powder, placing defatted cocoa powder in 55% ethanol solution, adding alkaline protease for hydrolysis reaction to obtain bioactive peptide-containing extractive solution, and lyophilizing to obtain cocoa small peptide extract.
Further, the reflux extraction time is 10-90min, and the reflux extraction temperature is 20-45 ℃.
Further, the centrifugal rotating speed is 500-6000r/min, and the centrifugal time is 10-15min.
Further, the addition amount of the alkaline protease is 0.1-2 wt% of the ethanol solution.
Further, the ratio of the defatted cocoa powder to the ethanol solution is 1:g/10-20mL.
Further, the hydrolysis pH is 8.0, the hydrolysis temperature is 45-50 ℃, and the hydrolysis time is 60-90min.
Further, the ultrafiltration cut-off has a molecular weight of 3kDa and a pressure of 3.5-8.0bar.
The cocoa extract of the present invention contains a abundance of bioactive peptides, all of which are derived from enzymatic hydrolysis of cocoa proteins. In addition, the invention also retains part of polyphenols, alkaloids and caffeine in the cocoa. The retention of the active ingredients endows the solid beverage of the invention with a certain antioxidant activity and a certain immunoregulatory effect.
Further, the probiotics comprise at least three of lactobacillus rhamnosus, bifidobacterium lactis, lactobacillus acidophilus, bacillus clausii and lactobacillus plantarum.
Further, the probiotics are added in the form of bacterial powder, wherein the number of viable bacteria in the lactobacillus rhamnosus bacterial powder is 3×10 8 The number of viable bacteria in CFU/g and bifidobacterium lactis powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g lactobacillus acidophilus bacterial powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g lactobacillus plantarum bacterial powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g and bacillus clausii powder is 1 multiplied by 10 8 CFU/g。
Further, the sweetener is one or more of stevioside, inulin, fructo-oligosaccharide, glucose-oligosaccharide, trehalose, sucralose and neotame.
Further, the vitamin is one or more of vitamin D, vitamin B6, vitamin B9, vitamin B12, vitamin C and vitamin E.
Further, the mineral is one or more of calcium, magnesium, sodium, potassium and zinc.
The invention also provides a preparation method of the hericium erinaceus polysaccharide small peptide probiotic solid beverage, which specifically comprises the following steps: mixing the raw materials with a V-shaped mixer, bagging and packaging to obtain the final product.
The brewing method of the hericium erinaceus polysaccharide small peptide probiotic solid beverage is that 200-250mL of water is added into each bag.
Further, the specification of the hericium erinaceus polysaccharide small peptide probiotic solid beverage is 3-10 g/bag.
Although the principle is not detailed, in the process of preparing and developing the product, the polysaccharide-rich extract obtained by taking the hericium erinaceus mycelium as the raw material can play the roles of an emulsifying agent and a stabilizing agent in the solid beverage, but the hericium erinaceus fruiting body polysaccharide extract does not have the effects. After the hericium erinaceus polysaccharide extract is added, the fineness of the solid beverage is improved, the dissolution speed is increased, and excessive foaming does not occur in the brewing and dissolution process to influence the appearance and the taste. In addition, the Hericium erinaceus fruiting body polysaccharide extract can improve the stability of solid beverage, prevent deterioration or discoloration due to external factors such as heat, light and oxygen, and prevent coagulation, bitter or sour after brewing. The two polysaccharides are used together in a specific proportion, so that the sensory quality of the solid beverage can be obviously improved.
Experiments show that the hericium erinaceus polysaccharide extract can slow down the metabolism rate of alcohol in a human body and the concentration of the alcohol in blood when being used together with the cocoa small peptide extract. The reason for this is probably that the combination of the two can reduce the activities of Alcohol Dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) to achieve the above-mentioned effects.
In addition, a small amount of glucose oxidase is added to the raw material. Glucose oxidase is an oxidoreductase that catalyzes the reaction of glucose under the action of oxygen to hydrogen peroxide and D-gluconolactone. This enzyme is produced by some fungi and insects and has antibacterial activity. Glucose oxidase comprises two subunits, each consisting of a polypeptide chain and a Flavin Adenine Dinucleotide (FAD), which is a cofactor for glucose oxidase, involved in electron transfer; two sugar chains, each sugar chain consisting of four monosaccharides, mannose, galactose, N-acetamido galactose and N-acetamido glucosamine, respectively, the sugar chains being linked to the polypeptide chains by an N-glycosylation reaction; two calcium ions, each forming a coordination bond with three carboxyl groups and one water molecule on the polypeptide chain of one subunit, play an important role in the stability and activity of glucose oxidase. In the invention, the glucose oxidase can adjust the composition and proportion of polysaccharide in the polysaccharide extract of the hericium erinaceus fruit body, thereby improving the health care effect of the product and the absorption efficiency in human body. The glucose oxidase can also consume dissolved oxygen, thereby reducing oxidative stress, protecting the activity and survival rate of probiotics in the solid beverage, generating hydrogen peroxide, inhibiting the growth of other microorganisms, improving the competitive advantage of the probiotics, hydrolyzing glucose, providing more carbon sources and energy for the probiotics, and promoting the growth and metabolism of the probiotics.
Finally, modulating an immune response refers to modulating the immune system's activity or ability to protect the body, which is associated with immune stimulation or immunosuppression. The primary task of the immune system is to combat human infections by pathogens such as fungi, bacteria, viruses, protozoa and parasites. The research shows that the solid beverage has certain immunity improving effect, can improve the resistance of human body to influenza, coronavirus and the like, and has certain inhibition effect on hypersensitivity reaction.
Compared with the prior art, the invention has the beneficial technical effects that:
the hericium erinaceus polysaccharide small peptide probiotic solid drink has good human body absorption and utilization rate, good immunoregulation function, and can slow down the metabolism rate of alcohol in a human body and the concentration of alcohol in blood, so that the harm of alcohol to the human body is reduced. The hericium erinaceus polysaccharide small peptide probiotic solid beverage has good stability, good sensory quality and flavor after being brewed, and can meet the preference of most consumers.
Detailed Description
The technical scheme provided by the invention is further described below by combining with the embodiment.
Example 1
A Hericium erinaceus polysaccharide small peptide probiotic solid beverage is composed of the following raw materials in parts by weight:
80 parts of hericium erinaceus polysaccharide extract, 50 parts of cocoa small peptide extract, 20 parts of probiotics and 2 parts of glucose oxidase.
The hericium erinaceus polysaccharide extract is composed of hericium erinaceus fruiting body polysaccharide and hericium erinaceus mycelium polysaccharide according to a weight ratio of 2:1.
The probiotics are lactobacillus rhamnosus, bifidobacterium lactis, lactobacillus acidophilus, bacillus clausii and lactobacillus plantarum, and are added in the form of bacterial powder, wherein the number of viable bacteria in the lactobacillus rhamnosus bacterial powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g and bifidobacterium lactis powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g lactobacillus acidophilus bacterial powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g lactobacillus plantarum bacterial powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g and bacillus clausii powder is 1 multiplied by 10 8 CFU/g；
The preparation method of the hericium erinaceus fruiting body polysaccharide extract comprises the following steps:
extracting Hericium erinaceus fruiting body with 5 times of water at 70deg.C under ultrasonic at 35000HZ for 3 hr, filtering with diatomite, concentrating under reduced pressure, and lyophilizing to obtain polysaccharide extract of Hericium erinaceus fruiting body.
The preparation method of the hericium erinaceus mycelium polysaccharide extract comprises the following steps:
grinding the freeze-dried hericium erinaceus mycelia into powder, sieving the powder with a 200-mesh sieve to obtain freeze-dried hericium erinaceus mycelia powder, and mixing the freeze-dried hericium erinaceus mycelia powder with 90% ethanol solution according to a feed-liquid ratio of 1g: mixing 25mL, ultrasonically extracting at 85KHz for 40min, centrifuging the extractive solution after extraction to obtain supernatant, mixing with 80% ethanol solution at a volume ratio of 1:10, ultrasonically extracting at 50KHz for 30min, centrifuging the extractive solution at 5000r/min for 15min after purification to obtain supernatant, concentrating under reduced pressure, and lyophilizing to obtain Hericium erinaceus polysaccharide extract.
The preparation method of the cocoa small peptide extract comprises the following steps:
uniformly mixing cocoa powder with 1g/10mL ethanol, reflux-extracting at 25 ℃ for 90min, centrifuging at 5000r/min for 15min to obtain defatted cocoa powder, adding the defatted cocoa powder into 55% ethanol solution, simultaneously adding 0.8wt% of Alcalase alkaline protease, hydrolyzing at 45 ℃ for 90min at pH of 8.0, wherein the feed liquid ratio of the defatted cocoa powder to the ethanol solution is 1:g/20mL to obtain an extract containing bioactive peptide, and freeze-drying the extract to obtain the cocoa small peptide extract.
Mixing the raw materials by a V-shaped mixer, bagging and packaging to obtain the finished hericium erinaceus polysaccharide small peptide probiotic solid beverage.
Example 2
The difference from example 1 is that: the material consists of the following raw materials in parts by weight: 80 parts of hericium erinaceus polysaccharide extract, 30 parts of cocoa small peptide extract, 10 parts of probiotics, 2 parts of glucose oxidase, 7 parts of sweetener (inulin), 2.5 parts of vitamin and 3.5 parts of mineral.
Example 3
The difference from example 1 is that: the material consists of the following raw materials in parts by weight: 60 parts of hericium erinaceus polysaccharide extract, 30 parts of cocoa small peptide extract, 5 parts of probiotics, 1 part of glucose oxidase, 1 part of sweetener (fructo-oligosaccharide), 1 part of vitamin and 1 part of mineral.
Example 4
The difference from example 1 is that: the material consists of the following raw materials in parts by weight: 80 parts of hericium erinaceus polysaccharide extract, 50 parts of cocoa small peptide extract, 30 parts of probiotics, 5 parts of glucose oxidase, 10 parts of sweetener (neotame), 5 parts of vitamin and 5 parts of mineral.
Example 5
The difference from example 1 is that: the material consists of the following raw materials in parts by weight: 80 parts of hericium erinaceus polysaccharide extract, 30 parts of cocoa small peptide extract, 5 parts of probiotics, 5 parts of glucose oxidase, 1 part of sweetener (oligoglucose), 5 parts of vitamin and 1 part of mineral.
Example 6
The difference from example 1 is that: the material consists of the following raw materials in parts by weight: 60 parts of hericium erinaceus polysaccharide extract, 50 parts of cocoa small peptide extract, 30 parts of probiotics, 3 parts of glucose oxidase, 2 parts of sweetener (trehalose), 3 parts of vitamin and 1 part of mineral.
Example 7
The difference from example 1 is that: the probiotics are bifidobacterium lactis, lactobacillus acidophilus, bacillus clausii and lactobacillus plantarum.
Example 8
The difference from example 1 is that: the probiotics are lactobacillus rhamnosus, lactobacillus acidophilus, bacillus clausii and lactobacillus plantarum.
Example 9
The difference from example 1 is that: the probiotics are lactobacillus rhamnosus, bifidobacterium lactis, bacillus clausii and lactobacillus plantarum.
Example 10
The difference from example 1 is that: the probiotics are lactobacillus rhamnosus, bifidobacterium lactis, lactobacillus acidophilus and lactobacillus plantarum.
Example 11
The difference from example 1 is that: the probiotics are lactobacillus rhamnosus, bifidobacterium lactis, bacillus clausii and lactobacillus acidophilus.
Example 12
The difference from example 1 is that: the probiotics are lactobacillus rhamnosus, bifidobacterium lactis and lactobacillus acidophilus.
Example 13
The difference from example 1 is that: the probiotics are lactobacillus rhamnosus, bifidobacterium lactis and bacillus clausii.
Example 14
The difference from example 1 is that: the probiotics are lactobacillus rhamnosus, bifidobacterium lactis and lactobacillus plantarum.
Example 15
The difference from example 1 is that: the probiotics are bifidobacterium lactis, bacillus clausii and lactobacillus plantarum.
Example 16
The difference from example 1 is that: the raw materials also comprise 5 parts of trehalose, 5 parts of vitamins and 1 part of minerals.
The vitamin is composed of vitamin B6, vitamin C and vitamin E according to the weight ratio of 1:2:1. The mineral substance consists of calcium and zinc according to the weight ratio of 3:1.
Comparative example 1
The difference from example 1 is that: hericium erinaceus polysaccharide extract is not added.
Comparative example 2
The difference from example 1 is that: the cocoa small peptide extract was not added.
Comparative example 3
The difference from example 1 is that: no probiotics were added.
Comparative example 4
The difference from example 1 is that: glucose oxidase was not added.
Test example 1 alcohol test
The test method comprises the following steps: the male and female half of 60 volunteers are selected, with body mass index of 19-21, age of 20-40 years, excluding drug, alcohol tolerance, alcohol intolerance, alcohol allergy, and smoker. Volunteers were randomly divided into 6 groups of 10 persons each, each half of men and women.
The first group took the product of example 1 of the present invention before drinking, the second group took the product of comparative example 1 of the present invention before drinking, the third group took the product of comparative example 2 of the present invention before drinking, the fourth group took the product of comparative example 3 of the present invention before drinking, the fifth group took the product of comparative example 4 of the present invention before drinking, and the sixth group took sterile water as placebo before drinking. The product is prepared by dissolving 20g of the corresponding product in 300mL of water, and allowing volunteers to drink before drinking. The drinking amount is based on the weight, and is specifically 1g/kg.
Ethanol content in blood of volunteers was measured with a breath analyzer 1, 3, and 5 hours before and after alcohol consumption, respectively.
The detection results are as follows:
therefore, the hericium erinaceus polysaccharide extract and the cocoa small peptide extract are used together, so that the hericium erinaceus polysaccharide extract and the cocoa small peptide extract have good effects of reducing the damage of alcohol to human bodies.
Test example 2 test for improving immunity
60 healthy volunteers were selected, aged between 20-50 years, and had no chronic or immune-related disease in both men and women. Volunteers were randomly divided into two groups of 30 persons each, each half of a man and a woman, one group being the experimental group and the other group being the control group.
The volunteers of the experimental group took 20g of the product of example 1 daily with water, and the control group took the same amount of sterile water as placebo daily at the same time for 21 days.
Samples of blood and saliva from volunteers were collected before the start of the test (day 0) and during the run of the test (days 7, 14, 21), and the content of IgA, igG, igM, C, C4 and CRP was determined by ELISA. The effect of the solid beverage of the present invention on the immune response of human body was evaluated by comparing the level change of immune-related factors of two groups of volunteers, and the specific results are as follows:
note that: data are mean ± standard deviation, indicating significant differences (p < 0.05) compared to control group, significant differences (p < 0.01), and extremely significant differences (p < 0.001).
The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present invention and the core ideas thereof; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (9)
1. The hericium erinaceus polysaccharide small peptide probiotic solid beverage is characterized by comprising the following raw materials in parts by weight:
60-80 parts of hericium erinaceus polysaccharide extract, 30-50 parts of cocoa small peptide extract, 5-30 parts of probiotics and 1-5 parts of glucose oxidase.
2. The hericium erinaceus polysaccharide small peptide probiotic solid beverage according to claim 1, wherein the preparation method of the hericium erinaceus polysaccharide extract is as follows:
freeze-drying Hericium erinaceus mycelium, grinding into powder, sieving with 200 mesh sieve to obtain Hericium erinaceus mycelium freeze-dried powder, mixing the Hericium erinaceus mycelium freeze-dried powder with ethanol solution, extracting for the first time, centrifuging the extractive solution after the extraction is finished to obtain supernatant, extracting and purifying the supernatant with ethanol solution for the second time, centrifuging the extractive solution after the purification to obtain supernatant, concentrating the supernatant under reduced pressure, and freeze-drying to obtain Hericium erinaceus polysaccharide extract.
3. The hericium erinaceus polysaccharide small peptide probiotic solid beverage according to claim 1, wherein the preparation method of the cocoa small peptide extract is as follows:
mixing cocoa powder and ethanol, extracting under reflux, centrifuging to obtain defatted cocoa powder, placing defatted cocoa powder in 55% ethanol solution, adding alkaline protease for hydrolysis reaction to obtain bioactive peptide-containing extractive solution, ultrafiltering, and lyophilizing to obtain cocoa small peptide extract.
4. The hericium erinaceus polysaccharide small peptide probiotic solid beverage according to claim 1, wherein the probiotics comprise at least three of lactobacillus rhamnosus, bifidobacterium lactis, lactobacillus acidophilus, bacillus clausii and lactobacillus plantarum.
5. The hericium erinaceus polysaccharide small peptide probiotic solid beverage according to claim 4, wherein the probiotics are added in the form of bacterial powder, wherein the number of viable bacteria in lactobacillus rhamnosus bacterial powder is 3×10 8 The number of viable bacteria in CFU/g and bifidobacterium lactis powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g lactobacillus acidophilus bacterial powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g lactobacillus plantarum bacterial powder is 3 multiplied by 10 8 The number of viable bacteria in CFU/g and bacillus clausii powder is 1 multiplied by 10 8 CFU/g。
6. The hericium erinaceus polysaccharide small peptide probiotic solid beverage according to claim 1, wherein the sweetener is one or more of stevioside, inulin, fructo-oligosaccharide, gluco-oligosaccharide, trehalose, sucralose and neotame.
7. The hericium erinaceus polysaccharide small peptide probiotic solid beverage according to claim 1, wherein the vitamins are one or more of vitamin D, vitamin B6, vitamin B9, vitamin B12, vitamin C and vitamin E.
8. The hericium erinaceus polysaccharide small peptide probiotic solid beverage according to claim 1, wherein the mineral is one or more of calcium, magnesium, sodium, potassium and zinc.
9. The method for preparing the hericium erinaceus polysaccharide small peptide probiotic solid beverage according to any one of claims 1 to 8, which is characterized by comprising the following steps: mixing the raw materials with a V-shaped mixer, bagging and packaging to obtain the final product.
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