CN116941485A - Method for overcoming low-temperature weak light stress of pepper seedlings - Google Patents
Method for overcoming low-temperature weak light stress of pepper seedlings Download PDFInfo
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- CN116941485A CN116941485A CN202211648417.XA CN202211648417A CN116941485A CN 116941485 A CN116941485 A CN 116941485A CN 202211648417 A CN202211648417 A CN 202211648417A CN 116941485 A CN116941485 A CN 116941485A
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- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 55
- 239000006002 Pepper Substances 0.000 title claims abstract description 50
- 235000016761 Piper aduncum Nutrition 0.000 title claims abstract description 50
- 235000017804 Piper guineense Nutrition 0.000 title claims abstract description 50
- 235000008184 Piper nigrum Nutrition 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 19
- 244000203593 Piper nigrum Species 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 60
- 241000722363 Piper Species 0.000 claims abstract description 49
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000004202 carbamide Substances 0.000 claims abstract description 27
- 230000003203 everyday effect Effects 0.000 claims abstract description 7
- 238000011282 treatment Methods 0.000 claims description 31
- 241000196324 Embryophyta Species 0.000 claims description 11
- 239000000758 substrate Substances 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 230000002354 daily effect Effects 0.000 claims description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 239000005416 organic matter Substances 0.000 claims description 2
- 229910052698 phosphorus Inorganic materials 0.000 claims description 2
- 239000011574 phosphorus Substances 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 7
- 238000005507 spraying Methods 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000002411 adverse Effects 0.000 abstract description 3
- 230000000243 photosynthetic effect Effects 0.000 description 13
- 239000011159 matrix material Substances 0.000 description 9
- 241000208293 Capsicum Species 0.000 description 4
- 239000001390 capsicum minimum Substances 0.000 description 4
- 230000002596 correlated effect Effects 0.000 description 2
- 238000010219 correlation analysis Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000306 recurrent effect Effects 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241000758706 Piperaceae Species 0.000 description 1
- IKGXIBQEEMLURG-UHFFFAOYSA-N Rutin Chemical compound OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-UHFFFAOYSA-N 0.000 description 1
- 241000033695 Sige Species 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G25/00—Watering gardens, fields, sports grounds or the like
- A01G25/16—Control of watering
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a method for overcoming low-temperature weak light stress of pepper seedlings, which is characterized in that water control is carried out at the beginning of a three-leaf one-heart period of the pepper seedlings, and simultaneously urea solution is sprayed every day during the water control period. The invention adopts the method of adversity exercise and urea solution spraying to improve the stress resistance of the pepper seedlings, completely overcomes the adverse effect of low-temperature weak light stress possibly occurring in the later period on the pepper seedlings, has simple operation, is suitable for being applied in production, and has higher application value.
Description
Technical Field
The invention relates to the technical field of agricultural planting, in particular to a method for overcoming low-temperature weak light stress of pepper seedlings.
Background
The capsicum is the vegetable with the largest planting area in China, is popular with Wen Xiguang, is sensitive to low-temperature weak light, and has the temperature lower than 15 ℃ or the light intensity lower than 200 mu mol.m −2 ·s −1 All can inhibit the growth and development of the plant, thereby resulting in yield reduction. In recent years, due to the influence of haze weather, even overcast weather and extremely low temperature weather, the probability of low-temperature weak light stress in the production of pepper seedlings is increased, so that the seedlings are weak and sick, the yield and the quality are reduced, and the efficient cultivation and the out-of-season production of the peppers are severely restricted. Therefore, the key problems of overcoming the low-temperature weak light adversity in the pepper seedling culture are solved, and the improvement of the yield and the quality of pepper harvest is facilitated.
Adversity exercise refers to that plants after being subjected to moderate adversity in the early stage show stronger resistance/tolerance to the recurrent adversity stress, which is also called adversity stress memory. Compared with the plants without exercise, the signal regulating substances, secondary metabolites, stress protective substances and the like of the plants with exercise can respond to the recurrent adversity stress more quickly and effectively, thereby enhancing the stress tolerance of the plants. At present, the application of stress exercise in production is few, only the application is mainly concentrated on field crops, and the application on pepper seedling cultivation is almost blank.
Disclosure of Invention
The invention aims to provide a method for overcoming low-temperature weak light stress of pepper seedlings, so as to solve the technical problems existing in the conventional pepper seedling.
The technical scheme adopted by the invention is as follows: a method for overcoming low-temperature weak light stress of pepper seedlings is characterized in that water is controlled in a three-leaf one-heart period of the pepper seedlings, and simultaneously urea solution is sprayed every day during the water control period.
Further, the water content of the matrix is controlled to be 65% -75% of the maximum water holding capacity, and meanwhile, the urea solution with the mass fraction of 1.39% -1.50% is sprayed every day in the water control period, and the water control time is 5 days.
Further, the water content of the substrate was controlled to 70% of the maximum water holding capacity, while a urea solution having a mass fraction of 1.50% was sprayed daily during the water control.
Further, the urea solution is uniformly sprayed until the blade tip drips when being sprayed.
Further, the pepper variety used is one of yangjiao No. 2, yangjiao No. 5 and yangjiao 1766.
Furthermore, the substrate for cultivating the capsicum seedlings has the following physical and chemical properties: quick-acting nitrogen: 600-2000 mg/kg; quick-acting phosphorus: 400-1500 mg/kg; quick-acting potassium: 5000-10000 mg/kg; weight by volume: 0.35-0.60 g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the Total porosity: 70.00-76.00%; organic matter content: 24.00-32.00%; pH value: 5.8-6.6; EC value: 1100-1600 us/cm.
Further, the seedling raising plug tray is a 50-hole standard plug tray.
Further, water control treatment is carried out at the beginning of the three-leaf one-heart period of the pepper seedling, the water content of the matrix is controlled to be 65-75% of the maximum water holding capacity, urea solution with the mass fraction of 1.39% -1.50% is sprayed every day during the water control period, water control is carried out for 5 days, and the maximum water holding capacity of the matrix is recovered for 3 days after the water control is finished.
Further, after the completion of the recovery, a low-temperature dim light treatment [ T, temperature 15 ℃ C./5 ℃ C. (day/night) and light intensity 100. Mu. Mol.m ] was performed −2 ·s −1 Photoperiod 12 h/12 h (day/night)]After the warm light treatment was completed, the growth index and the strong seedling index were measured, wherein the strong seedling index= (stem thickness/plant height+dry weight of the subsurface/dry weight of the aerial) x dry weight of the plants.
The invention has the advantages that: the invention adopts the method of adversity exercise and urea solution spraying to improve the stress resistance of the pepper seedlings, completely overcomes the adverse effect of low-temperature weak light stress possibly occurring in the later period on the pepper seedlings, has simple operation, is suitable for being applied in production, and has higher application value.
Drawings
FIG. 1 is a graph showing the net photosynthetic rate of the top-expanded leaf of example 1.
FIG. 2 is a graph showing the correlation of the strong index of pepper seedlings with the net photosynthetic rate of the top-expanding leaf in example 1.
FIG. 3 is a graph showing the net photosynthetic rate of the top-expanded leaf of example 2.
FIG. 4 is a graph showing the correlation of the strong index of pepper seedlings with the net photosynthetic rate of the top-expanding leaf in example 2.
FIG. 5 shows an index chart of the concentration of the urea solution sprayed in example 2.
Detailed Description
Example 1:
the seedling is carried out in a seedling temperature chamber of the Sige basket base of the urban area of Yangzhou in 2022 month by taking Yangjiao No. 5 as a material, and the seedling mode is conventional plug seedling. Different water control treatments are started in three-leaf one-heart period (about 28 days after sowing, slightly different days are affected by the change of environmental factors in a greenhouse), the water content of the matrix is controlled to be 65% -75% of the maximum water holding capacity, and the water control mode can be realized by adopting weighing and then supplementing water or supplementing water according to the measurement result of a soil moisture meter for 5 days. After the end of the water control, the matrix was recovered for 3 days with maximum water holding capacity (100%). After the recovery, a low-temperature dim light treatment [ T ] was performed at 15 ℃ per 5 ℃ (day/night) with an illumination intensity of 100. Mu. Mol.m −2 ·s −1 Photoperiod 12 h/12 h (day/night)](the condition is general treatment of low-temperature weak light stress of capsicum), and the normal temperature light [ CK, the temperature is 25 ℃/15 ℃ (day/night), and the illumination intensity is 300 mu mol.m −2 ·s −1 Photoperiod 12 h/12 h (day/night)](this condition is the general treatment of normal temperature light of capsicum) as a control. After the warm light treatment was completed, the growth index and the strong seedling index were measured, wherein the strong seedling index= (stem thickness/plant height+dry weight of the subsurface portion/dry weight of the aerial portion) ×dry weight of the plant, and the results are shown in table 1.
It can be seen that, under the low-temperature weak light treatment, as the water content of the matrix is reduced, the growth index and the strong seedling index of the pepper seedlings are in a trend of rising first and then falling, which indicates that moderate water control can improve the resistance of the pepper seedlings, and is favorable for relieving the reduction of the strong seedling index of the pepper by the low-temperature weak light treatment, wherein the effect of treating the pepper seedlings with 70% of the maximum water holding capacity of the matrix is the best. However, it can be seen from the table that the moderate water control still cannot completely overcome the low temperature weak light stress, and further needs to be matched with other treatments (it should be noted that, as known to those skilled in the art, the results shown in table 1 are the results of one seedling raising, and even if the seedling raising is performed on the same land in the same treatment mode, the seedling raising conditions cannot be strictly identical, so that the seedling raising results of each time have differences, but this does not affect the technical effects of table 1 on different treatment modes).
TABLE 1 growth index and strong seedling index manifestation
Further, we examined the net photosynthetic rate of the top-spread leaves of the pepper seedlings, which showed a trend of rising and then falling with decreasing water content of the substrate under low temperature weak light treatment, indicating that moderate water control can improve photosynthetic capacity of the pepper seedling leaves, wherein the treatment with 70% of the maximum water holding capacity of the substrate is the best.
In addition, correlation analysis shows that the strong seedling index of the pepper seedlings is obviously and positively correlated with the net photosynthetic rate of the top-expanding leaves (the determination coefficient reaches 0.8853), and the moderate water control can enable the pepper seedlings to maintain stronger photosynthetic capacity, so that the decrease of the strong seedling index caused by low-temperature weak light stress is relieved.
Example 2:
further, the seedling is carried out in 2022 and 4 months in a seedling temperature chamber of the Sophorin vegetable basket base in the urban area of Yangzhou by taking Yangjiao No. 5 as a material, and the seedling mode is conventional plug seedling. The water control treatment is started in three-leaf one-heart period (about 28 days after sowing, slightly different days are affected by the change of environmental factors in the greenhouse). Under the condition of controlling the maximum water holding capacity of 70% of the matrix, urea solutions with different concentrations are sprayed every day during the water control period, and the urea solutions are sprayed at about 17:00 and uniformly sprayed until the water is dropped from the blade tip, and the water is controlled for 5 days. After the water control is finished, the maximum water holding capacity of the matrix is recovered for 3 days. After recovery, low temperature dim light treatment [ T, temperature 15 ℃/5 ℃ (day/night), illumination intensity100 mu mol m −2 ·s −1 Photoperiod 12 h/12 h (day/night)]And light at normal temperature [ CK ] at 25deg.C/15deg.C (day/night) with an illumination intensity of 300 μmol.m −2 ·s −1 Photoperiod 12 h/12 h (day/night)]Is used as a control. After the warm light treatment was completed, the growth index and the strong seedling index were measured, and the results are shown in table 2.
It can be seen that under the low-temperature weak light treatment, as the spraying concentration of the urea solution increases, the growth index and the strong seedling index of the pepper seedlings tend to rise first and then fall, and the treatment effect of the urea concentration of 1.5% is the best. Meanwhile, under the treatment, the growth index and the strong seedling index of the pepper seedling are larger than those of normal temperature light treatment, which shows that the water is controlled by adopting 70 percent of the maximum water holding capacity of the substrate and the urea solution with the mass fraction of 1.5 percent is sprayed, so that the adverse effect of low temperature weak light stress on the pepper seedling can be completely overcome, and the normal growth state of the pepper seedling is achieved.
TABLE 2 growth index and strong seedling index table
Further, we measured the net photosynthetic rate of the top-spread leaves of the pepper seedlings treated differently, and can see that the net photosynthetic rate of the top-spread leaves of the pepper seedlings tends to rise and then fall with the increase of the spraying concentration of the urea solution under the low-temperature weak light treatment, which indicates that spraying urea with a proper concentration can improve the photosynthetic capacity of the leaves of the pepper seedlings, wherein the effect of the treatment with the concentration of 1.5% urea is the best.
In addition, correlation analysis shows that the strong seedling index of the pepper seedlings is extremely obviously and positively correlated with the net photosynthetic rate of the top-expanding leaves (the determination coefficient reaches 0.9672), and the condition that the photosynthetic capacity of the seedlings can be improved by spraying urea solution with proper concentration is shown, so that the strong seedling index is improved.
Further, by fitting the data with the concentration of the sprayed urea solution as the abscissa and the strong seedling index as the ordinate, the trend of the strong seedling index with the concentration of the sprayed urea solution as a unitary four-time equation can be seen, and the equation is y= 149765x 4 - 9824.7x 3 + 161.04x 2 0.0971 x+0.0551, the coefficient rj= 0.9361 is determined to a significant level. The strong seedling index of the control treatment (CK+0%) is substituted into the equation to obtain the concentration of the corresponding spray urea solution which is 1.3875%. Therefore, the concentration of the urea solution sprayed is controlled between 1.39% and 1.50%, and the seedling strengthening index can reach or exceed that of the control treatment (CK+0%).
Claims (9)
1. A method for overcoming low-temperature weak light stress of pepper seedlings is characterized in that water control is carried out at the beginning of three-leaf one-heart period of pepper seedlings, and meanwhile urea solution is sprayed every day during the water control period.
2. The method for overcoming the low-temperature weak light stress of the pepper seedlings as recited in claim 1, wherein the water content of the substrate is controlled to be 65% -75% of the maximum water holding capacity, and the urea solution with the mass fraction of 1.39% -1.50% is sprayed daily during the water control period, and the water control time is 5 days.
3. A method for overcoming the low-temperature weak light stress of pepper seedlings as claimed in claim 2, wherein the water content of the substrate is controlled to be 70% of the maximum water holding capacity, and simultaneously the urea solution with the mass fraction of 1.50% is sprayed daily during the water control period.
4. The method for overcoming the low-temperature weak light stress of the pepper seedlings as recited in claim 1, wherein the urea solution is uniformly sprayed until the water drops from the leaf tips.
5. The method for overcoming the low-temperature weak light stress of pepper seedlings as claimed in claim 1, wherein the pepper variety used is one of yangjiao No. 2, yangjiao No. 5 and yangjiao 1766.
6. The method for overcoming the low-temperature weak light stress of the pepper seedlings as recited in claim 1, wherein the substrate for the pepper seedlings has the following physical and chemical properties: quick-acting nitrogen: 600-2000mg/kg; quick-acting phosphorus: 400-1500 mg/kg; quick-acting potassium: 5000-10000 mg/kg; weight by volume: 0.35-0.60 g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the Total porosity: 70.00-76.00%; organic matter content: 24.00-32.00%; pH value: 5.8-6.6; EC value: 1100-1600 us/cm.
7. The method for overcoming the low-temperature weak light stress of the pepper seedlings as recited in claim 1, wherein the seedling raising plug is a 50-hole standard plug.
8. The method for overcoming the low-temperature weak light stress of the pepper seedlings according to claim 1, wherein the water control treatment is carried out at the beginning of the three-leaf one-heart period of the pepper seedlings, the water content of the substrate is controlled to be 65-75% of the maximum water holding capacity, the urea solution with the mass fraction of 1.39% -1.50% is sprayed every day during the water control period, the water control is carried out for 5 days, and the maximum water holding capacity of the substrate is recovered for 3 days after the water control is finished.
9. The method for overcoming the low-temperature weak light stress of the pepper seedlings as recited in claim 8, wherein the low-temperature weak light treatment [ T ] is performed after the recovery is finished, the temperature is 15 ℃/5 ℃ (day/night), and the illumination intensity is 100 mu mol m −2 ·s −1 Photoperiod 12 h/12 h (day/night)]After the warm light treatment was completed, the growth index and the strong seedling index were measured, wherein the strong seedling index= (stem thickness/plant height+dry weight of the subsurface/dry weight of the aerial) x dry weight of the plants.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211648417.XA CN116941485A (en) | 2022-12-21 | 2022-12-21 | Method for overcoming low-temperature weak light stress of pepper seedlings |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211648417.XA CN116941485A (en) | 2022-12-21 | 2022-12-21 | Method for overcoming low-temperature weak light stress of pepper seedlings |
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| Publication Number | Publication Date |
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| CN116941485A true CN116941485A (en) | 2023-10-27 |
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| CN202211648417.XA Pending CN116941485A (en) | 2022-12-21 | 2022-12-21 | Method for overcoming low-temperature weak light stress of pepper seedlings |
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- 2022-12-21 CN CN202211648417.XA patent/CN116941485A/en active Pending
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