CN116924573A - Culture method of algae symbiotic granular sludge - Google Patents
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture method of a algae symbiotic granular sludge, which aims to solve the problem of low grease yield of the algae symbiotic granular sludge system by exploring the influence of different light-dark ratios on the algae symbiotic granular sludge (Algal-bacterial granular sludge, ABGS) system, and experiments find that the light irradiation time of 16h is favorable for the growth of microalgae, so that the algae symbiotic granular sludge can generate more chlorophyll and grease, and the particle sludge with high grease content can be obtained by the light-dark ratio of 16h/8h in consideration of the grease content of residual sludge and the energy consumption problem of system operation, thereby having industrial application value.
Description
Technical Field
The invention relates to the technical field of environmental protection, in particular to a culture method of a fungus and algae symbiotic granular sludge.
Background
The most widely used wastewater treatment technology at present is an activated sludge method, but the sedimentation performance is poor, the mud-water separation effect is poor, and the sludge expansion is easy to occur; sensitive to impact load, and difficult to adapt to the change trend of water quality diversification; the large amount of excess sludge is liable to burden subsequent sludge disposal. Compared with activated sludge, the aerobic granular sludge (Aerobic granular sludge, AGS) has compact structure, high sedimentation rate and simple sludge-water separation, does not need to be additionally provided with a secondary sedimentation tank, and is a new development direction of activated sludge technology. In addition, AGS has the advantages of rich microorganism types, large biomass, synchronous denitrification and dephosphorization, strong impact load resistance and the like which are incomparable with the activated sludge method, but aerobic granular sludge is the same as the activated sludge, a large amount of excess sludge is generated in the operation process, the additional value of the excess sludge is very low, and the subsequent application path is limited.
The algae symbiotic technology is an emerging sewage treatment technology, algae cells convert nutrient elements in sewage into biomass through photosynthesis and release oxygen, and the oxygen is utilized by symbiotic bacteria to generate nutrient substances and carbon dioxide required by algae photosynthesis, so that the algae symbiotic sewage treatment technology can realize high-efficiency removal of pollutants and synchronous reduction of aeration energy consumption. In addition, microalgae cells can accumulate grease and are considered as potential alternative energy sources. The algae symbiosis can improve the added value of the residual sludge and provide feasibility for sewage treatment and recycling.
Based on the aerobic granular sludge technology and the algae symbiotic system, the established algae symbiotic granular sludge (Algal-bacterial granular sludge, ABGS) system not only has the advantages of excellent precipitating performance, high biological density, high-efficiency denitrification and dephosphorization capability and the like of AGS, but also effectively relieves the problems of difficult collection of suspended microalgae, poor stability of granular sludge, lower added value and the like, and is a sewage treatment strategy with great application prospect. However, during long-term operation of the mycotic symbiotic granular sludge, the granular sludge can generate a large amount of excess sludge, and the part of sludge has the problem of low grease content.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a culture method of the mycotic and algal symbiotic granular sludge, which improves the mycotic and algal symbiotic relationship of the granular sludge by adjusting different light-dark ratios and solves the technical problem of low grease content of the conventional mycotic and algal symbiotic granular sludge.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a culture method of a mycotic symbiotic granular sludge comprises the following steps:
s1, inoculating flocculent sludge into an SBR reactor, wherein the reactor comprises a light source, a peristaltic pump, an aeration stone and a rotameter;
s2, selecting a light source as an LED lamp post, and controlling the light-dark ratio;
s3, adopting artificial simulated wastewater as water inlet of the SBR reactor, glucose and sodium acetate as carbon sources, ammonium chloride as nitrogen sources, monopotassium phosphate as phosphorus sources, and adding NaHCO into the water inlet 3 The pH of the solution is kept within the range of 7.0-8.0, and trace elements required by the growth of microorganisms are supplied at the same time;
s4, the SBR reactor operates in a water inlet-standing-aeration-precipitation-water outlet-idling mode, 4 hours is a period, and the total period is 6, wherein water is fed for 1-5min, standing is carried out for 30-40min, aeration is carried out for 160-190min, precipitation is carried out for 1-20min, water is discharged for 1-5min, and idling is carried out for 10-15min;
s5, after the SBR reactor is operated for 50 days, the mycotic symbiotic granular sludge can be formed.
Preferably, in step S2, the light-dark ratio is selected to be 0h/24h, 8h/16h, 16h/8h or 24h/0h; further preferably 16h/8h.
Preferably, in step S2, the illumination intensity of the LED lamp post is 193.58 mu mol/m 2 /s。
Preferably, in the step S3, the concentration of COD in the artificial simulated wastewater is 500-800mg/L, NH 4 + The concentration of the-N is 50-200mg/L, PO 4 3- The concentration of P is 5-20mg/L.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the influence of different light-dark ratios on a algae symbiotic granular sludge (Algal-bacterial granular sludge, ABGS) system is explored, so that the problem of low grease yield of the algae symbiotic granular sludge system is solved, the experiment shows that the light irradiation time of 16 hours is favorable for the growth of microalgae, the algae symbiotic granular sludge can generate more chlorophyll and grease, the problem of residual sludge grease content and system operation energy consumption is considered, and the light-dark ratio of 16 hours/8 hours can be used for obtaining granular sludge with high grease content, so that the algae symbiotic granular sludge has industrial application value.
Drawings
FIG. 1 is a diagram of an ABGS system culture experiment reaction apparatus under different light-dark ratios, wherein: 1. manually simulating the wastewater; 2. a water inlet pump; 3. a light shield; 4. a light source; 5. an aeration pump; 6. a flow meter; r1, the light-dark ratio is 0h/24h; r2, the light-dark ratio is 8h/16h; r3, the light-dark ratio is 16h/8h; r4, the light-dark ratio is 24h/0h;
fig. 2 is a graph showing the chlorophyll a content change trend of the granular sludge of each reaction system.
Detailed Description
The present invention will be described in further detail with reference to the following preferred examples, but the present invention is not limited to the following examples.
Unless otherwise specified, the chemical reagents involved in the present invention are all commercially available.
The inoculated sludge adopted by the invention is taken from an aeration tank of a water treatment plant of Wuhan city Jiang Xiawu, and the inoculated sludge is flocculent fluid with dark brown color and has rich biological phases. Aeration was first continued for 72 hours prior to sludge inoculation, with the initial mixed liquor suspension solids concentration (MLSS) of all reactors being approximately 13.3g/L, sludge Volume Index (SVI) 30 ) The concentration of the microalgae in the reactor was 27.7ml/g, the concentration of the MLVSS/MLSS was 0.56, and the microalgae in the reactor were all autogenous.
The artificial simulated wastewater is used as the water inlet of the SBR reactor to ensure the stability of the water quality of the water inlet of the experiment and the accuracy of the experiment, glucose and sodium acetate are respectively used as carbon sources, ammonium chloride is used as a nitrogen source, monopotassium phosphate is used as a phosphorus source, and NaHCO is added into the water inlet 3 The pH of the solution is maintained within 7.0-8.0, and trace elements such as Ca required for microorganism growth are supplied 2+ 、Mg 2+ 、Fe 2+ The composition of the artificial simulated wastewater quality is shown in Table 1:
table 1 artificial simulation of wastewater quality composition
The experiment is carried out in four cylindrical SBR reactors made of transparent organic glass as shown in figure 1, the height of each reactor is 0.6m, the diameter is 0.06m, the effective working volume is 1.6L, the reactor is composed of a light source, an air inlet pump, an air aeration pump, an aeration stone, a rotameter and other parts, and the water inlet and outlet, aeration and standing of the reactor are controlled by a time controller.
The bottom of the reactor is provided with an aeration stone, and the aeration rate is controlled to be about 2 L.min by a rotameter -1 The illumination of each reactor is provided by the same full-spectrum lamp post, and the illumination intensity of the LED lamp post is 193.58 mu mol/m 2 S (18 w, cct=4316k), a time controller was provided for controlling the illumination time of the reactor.
The four reactors are named as R1, R2, R3 and R4 respectively, the light-dark ratio is 0h/24h, 8h/16h, 16h/8h and 24h/0h respectively, and in the experimental process, in order to prevent the illumination between the reactors from affecting each other, a light shield is arranged on the corresponding reactor, and the temperature of the laboratory is controlled to be about 25+/-2 ℃.
The culture operation process of the ABGS system of the experiment is shown in table 2, the stage is divided into six stages of water inlet, standing, aeration, precipitation, water outlet and idle, 4 hours is one period, 6 periods are taken, as the seed sludge at the starting of the experiment is flocculent activated sludge, the precipitation time is periodically regulated and controlled to achieve the adaptation effect of the sludge to the environment in 5 days before the initial stage of the experiment, the precipitation time is kept unchanged for 5 minutes until more flocculent microorganisms appear in the reactor before and after the 52 th day of the experiment, the precipitation time is changed to 3 minutes later, the experimental aeration is measured by a flowmeter, and the intensity is controlled to be 2 L.min -1 About, the volume exchange rate of the reactor is 50%; the illumination intensity is controlled at 193.58 mu mol/m 2 /s。
TABGS system culture experiment operation conditions under different light-dark ratios
Extraction and detection of chlorophyll:
the extraction and detection of chlorophyll in the bacterial algae granular sludge in the four reactors R1, R2, R3 and R4 comprises the following specific steps:
since microalgae are autogenous in the experimental process, chlorophyll content in the system can be measured, and the measurement is based on national standard (GB 17378.7-2007), and the specific steps are as follows: taking V mL of sludge mixed solution after aeration for 30min, adding acetone according to the ratio of the mixed solution to acetone=9:1, sufficiently oscillating, and then putting into a refrigerator for refrigerating for 24h to extract chlorophyll; centrifuging at 4000r/min for 10min, taking supernatant carefully, and measuring absorbance at 750nm, 664nm, 647nm and 630nm respectively with "9+1" acetone solution as reference; subtracting the absorbance at 750nm from the absorbance at 664nm, 647nm, 630nm to obtain absorbance E 664 、E 647 、E 630 Then the chlorophyll a content is calculated according to the formula (1-1), L is the optical path of the measuring cell, and the optical path is 1cm.
As shown in FIG. 2, the change condition of microalgae can be seen through the change trend of chlorophyll a, microalgae are inoculated at the initial stage of experiments, the chlorophyll content is measured at 17d, the chlorophyll a content in the R1-R4 system is respectively 0.04 mug/g.SS, 0.03 mug/g.SS, 0.02 mug/g.SS and 0.19 mug/g.SS, the chlorophyll content of R1 is almost undetectable from the graph, the chlorophyll a content in R1 is lower than the detection limit, the microalgae which does not grow in R1 is related to the condition that the microalgae are always cultivated in darkness, green microalgae are not seen when appearance analysis is performed on sludge in the R1 system, R2 is cultivated under 8h condition, the chlorophyll a content in the R2 is always less until the chlorophyll a content in the R2 rises from 0.02 mug/g.SS to 0.30 mug/g.59 d, and positive effects on the algae particles in the system are obviously promoted when the algae particles in the R2 are increased.
The chlorophyll a content in R3 increases slowly from 31d to 52d, and the increasing trend is far higher than that of other reaction systems, and finally reaches 11.12 mug/g.SS which is 556 times of 17d, which shows that the microalgae content in the R3 system is the most, the illumination time length 16h is the most beneficial to the growth of microalgae, so that the ABGS of the R3 system is found to be dark green when the appearance morphological analysis is carried out on the ABGS of the R3 system, although the chlorophyll a of the R4 system is in a stable increasing state from the beginning to the end of the experiment and is always higher than the quantities of R1 and R2, the chlorophyll a content of the R4 system is measured to be one half of that of the R3 system at the end of the experiment, and is 5.03 mug/g.SS and 3.70 mug/g.SS of R2, the difference between the two is large, and the chlorophyll a change rule of the R2-R4 system is R3 > R4 > R2.
Extraction and determination of oil content
The extraction and determination of the grease content of the algae granule sludge in the four reactors R1, R2, R3 and R4 adopts an improved solvent extraction method, and the specific operation method is as follows:
(1) Firstly, freeze-drying the obtained bacterial algae granule sludge mixed liquor to obtain algae powder, weighing about 5g of the dried algae powder (m) after treatment, grinding the algae powder, and then placing the algae powder into a 5mL centrifuge tube;
(2) 5mL of ethyl acetate was added to the centrifuge tube: n-hexane=1: 1, and then carrying out ultrasonic crushing treatment in an ultrasonic crusher for at least 20min;
(3) Extracting the centrifuge tube in the step (2) in a water bath for 1h at the temperature of 60 ℃ of an electric heating constant temperature water bath kettle, and vibrating the centrifuge tube at any time in the extraction process to promote extraction in order to uniformly mix the solute and the solvent in the tube;
(4) Centrifuging for 20min with a table centrifuge 12000r/min, collecting supernatant into another centrifuge tube, extracting twice repeatedly, and mixing extractive solutions;
(5) Preparing a pieceOven-drying to constant weight glass test tube with weight of G 1 Adding distilled water with equal volume into the combined extracting solution, shaking uniformly, centrifuging for 10min at 3000r/min in a centrifuge, and collecting upper organic phase into a prepared glass tube;
(6) Placing the glass tube into an electric heating constant temperature water bath kettle, and evaporating in water bath at 85 ℃ for 2.5 hours until no liquid exists; taking out the glass tube, putting the glass tube into an electrothermal blowing drying oven, drying for 2 hours, and finally weighing the total mass of the glass tube dried to constant weight, wherein the weight is G 2 ；
(7) The crude fat content C (%), c=100× (G) 2 -G 1 )/m；
The experiment determines the oil content of microalgae in the end-stage bacterial-algal symbiotic granular sludge to obtain the average oil content in the R1-R4 system of 2.69mg/g, 4.46mg/g, 6.19mg/g and 5.73mg/g respectively, and according to the research, the quantity of the microalgae, namely the content of chlorophyll a, is in direct proportion to the oil content of the microalgae, the change rule of the chlorophyll a, which is determined by combining the experiment, is R3 > R4 > R2, and the change rule of the microalgae is consistent with the change rule of the system oil content, and the change rule of the oil content is R3 > R4 > R2 > R1.
Finally, it should be noted that: the above examples are not intended to limit the present invention in any way. Modifications and improvements will readily occur to those skilled in the art upon the basis of the present invention. Accordingly, any modification or improvement made without departing from the spirit of the invention is within the scope of the invention as claimed.
Claims (5)
1. The culture method of the mycosis granular sludge is characterized by comprising the following steps of:
s1, inoculating flocculent sludge into an SBR reactor, wherein the reactor comprises a light source, a peristaltic pump, an aeration stone and a rotameter;
s2, selecting a light source as an LED lamp post, and controlling the light-dark ratio;
s3, adopting artificial simulated wastewater as water inlet of the SBR reactor, glucose and sodium acetate as carbon sources, and ammonium chloride as carbon sourcesAs nitrogen source, potassium dihydrogen phosphate is taken as phosphorus source, naHCO is added into the inflow water 3 The pH of the solution is kept within the range of 7.0-8.0, and trace elements required by the growth of microorganisms are supplied at the same time;
s4, the SBR reactor operates in a water inlet-standing-aeration-precipitation-water outlet-idling mode, 4 hours is a period, and the total period is 6, wherein water is fed for 1-5min, standing is carried out for 30-40min, aeration is carried out for 160-190min, precipitation is carried out for 1-20min, water is discharged for 1-5min, and idling is carried out for 10-15min;
s5, after the SBR reactor is operated for 50 days, the mycotic symbiotic granular sludge can be formed.
2. The method for culturing the mycotic symbiotic granular sludge of claim 1 wherein in step S2, the light-dark ratio is selected to be 0h/24h, 8h/16h, 16h/8h or 24h/0h.
3. The method for culturing the mycosis granular sludge according to claim 2, wherein the light-dark ratio is preferably 16h/8h.
4. The method for culturing the mycoplasmas and the algae symbiotic granular sludge according to claim 1, wherein in the step S2, the illumination intensity of the LED lamp post is 193.58 mu mol/m 2 /s。
5. The method for culturing the symbiotic granular sludge of claim 1 wherein in step S3, the concentration of COD in the artificial simulated wastewater is 500-800mg/L, NH 4 + The concentration of the-N is 50-200mg/L, PO 4 3- The concentration of P is 5-20mg/L.
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