CN116919871A - Preparation method of extract of Panax ginseng C.A. Meyer with repairing and moisturizing effects, and product and application thereof - Google Patents
Preparation method of extract of Panax ginseng C.A. Meyer with repairing and moisturizing effects, and product and application thereof Download PDFInfo
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- CN116919871A CN116919871A CN202310972006.4A CN202310972006A CN116919871A CN 116919871 A CN116919871 A CN 116919871A CN 202310972006 A CN202310972006 A CN 202310972006A CN 116919871 A CN116919871 A CN 116919871A
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention relates to a preparation method of a ginseng extract with repairing and moisturizing effects, and a product and application thereof, comprising the following steps: pulverizing rhizoma Panacis Quinquefolii, extracting with 60-80% ethanol water solution under reflux, filtering, concentrating, purifying with macroporous resin, concentrating, flocculating and decolorizing, and drying. The extract prepared by the preparation method has the advantages of light color, less impurities and high extraction efficiency of effective active ingredients; the extract of the Panax ginseng C.A. Mey prepared by the preparation method can be applied to cosmetics with skin moisturizing or skin repairing effects.
Description
Technical Field
The invention belongs to the technical field of plant extraction, relates to a preparation method of a ginseng extract, a product and application thereof, and in particular relates to a preparation method of a ginseng extract with repairing and moisturizing effects, a product and application thereof.
Background
The rhizome of Panax ginseng is dried rhizome of Panax ginseng of Panax of Araliaceae, has Buddha bead shape or sphere-like shape, is a variety of Panax japonicus, is mainly produced in higher altitude areas such as Yunnan, guizhou, sichuan, etc., and is one of Yunnan Ben Cao and Taibai Qiu Yao, and has effects of nourishing lung and yin, removing blood stasis and relieving pain, and stopping bleeding. Modern researches have shown that, the rhizome of the root of the tuber wherein the main chemical component is triterpenoid saponin and is also main active component, the method mainly comprises oleanane type saponins such as ginsenoside IVa, ginsenoside IV, ginsenoside V (ginsenoside R0) and the like; researches indicate that the panax japonicus saponin IVa has the effect of promoting autophagy of cells and shows remarkable anti-aging potential, and researches prove that the ginsenoside R0 has the effect of inhibiting the generation of MMP-2 in fibroblasts induced by UVB and has remarkable effect of resisting skin photoaging.
At present, the extraction and purification process of the effective components in the rhizoma panacis majoris mainly comprises an alcohol extraction method, an ultrasonic auxiliary extraction method and the like. For example, CN101947249a discloses a method for extracting total saponins of rhizoma panacis majoris by ultrasonic wave, oven drying the rhizoma panacis majoris, pulverizing, sieving with 40 mesh sieve, weighing coarse powder, placing into soxhlet extractor, adding petroleum ether, refluxing for 2h, taking out filter paper tube, and air drying the medicinal material for use; adding 30% -70% ethanol, shaking, soaking for 1 hr, and ultrasonic extracting at 30-50deg.C for 10-30min. However, the ultrasonic assisted extraction has high energy consumption, is not suitable for industrial production, and has low content of active ingredients in the obtained extract. CN102603851a discloses a method for extracting purified panax japonicus saponin IVa from panax japonicus, which comprises the steps of properly crushing the panax japonicus, extracting with ethanol water solution or methanol water solution, filtering the extracting solution, concentrating under reduced pressure to obtain a paste, dispersing the paste in water, extracting with an organic solvent, concentrating to obtain a concentrated solution, dispersing with water, and separating the dispersed solution by using a macroporous resin column and high-speed countercurrent chromatography to prepare the high-concentration panax japonicus saponin IVa. However, the obtained product has heavy color, more impurities and difficult quality to ensure stability.
In addition, the application of the effective components of the rhizoma panacis majoris to skin repair and moisture preservation has been recently reported, aquaporin-3 AQP3 plays a key role in the process of playing a role in keratinocyte function, plays an important role in skin hydration, plays an important role in maintaining skin moisture and skin functions, can promote proliferation and migration of human keratinocyte, and is closely related to the generation and repair of skin barrier.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a preparation method of a ginseng extract, a product and application thereof, in particular to a preparation method of a ginseng extract with repairing and moisturizing effects, a product and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a method for preparing a extract of a panaxacum having a moisturizing effect, the method comprising the steps of:
(1) Pulverizing rhizoma Panacis Quinquefolii, extracting with 60-80% ethanol water solution under reflux, filtering the extractive solution, and concentrating to obtain crude extract;
(2) Purifying and eluting the crude extract of the rhizoma panacis majoris by macroporous resin, and collecting eluent;
(3) Concentrating the eluent, performing flocculation treatment and decoloration treatment, and then drying to obtain the extract of the rhizoma panacis majoris with the effects of repairing and moisturizing.
The 60-80% ethanol aqueous solution may be an ethanol aqueous solution with a concentration of 60%, 65%, 70%, 75%, 80%, etc., and other specific values within the numerical range may be selected, which will not be described in detail herein.
The preparation method of the extract of the Panax ginseng C.A. Meyer adopts the modes of ethanol hot extraction, macroporous resin purification and enrichment and flocculation treatment and decoloration treatment creatively, so that the prepared extract has the advantages of light color, less impurities and high extraction efficiency of effective active ingredients (mainly ginsenoside R0, ginsenoside IVa and polysaccharide); on the other hand, the obtained extract also has excellent effect of promoting the expression of aquaporin-3 in epidermal cells and excellent effect of promoting epidermal repair, so that the extract of the panaxacum majus L prepared by the preparation method can be applied to cosmetics with skin moisturizing or skin repairing effects.
Preferably, the macroporous resin comprises any one of SP850, HP2MGL or X-5.
Compared with other types of macroporous resin, the preparation method adopts the specific macroporous resin type, the content of the effective active ingredients (mainly ginsenoside R0, ginsenoside IVa and polysaccharide) of the prepared rhizoma panacis majoris extract and the total yield of the extract are obviously improved, and the preparation method has more excellent skin moisturizing and repairing effects.
Preferably, the purification elution process is as follows: eluting front-end impurities or non-eluting front-end impurities with 5-7BV 0-10% ethanol water solution, eluting 5-7BV with 35-45% ethanol water solution, and collecting the eluate (0% ethanol water solution is pure water).
The concentration and the total extraction content of the effective active ingredients (mainly ginsenoside R0, ginsenoside IVa and polysaccharide) can be obviously improved by adopting the specific elution procedure, namely the ethanol water solution with specific concentration in a matching way.
The 0-10% ethanol aqueous solution may be an ethanol aqueous solution with a concentration of 0% (pure water), 2%, 5%, 8%, 10%, etc., the 35-45% ethanol aqueous solution may be an ethanol aqueous solution with a concentration of 35%, 37%, 40%, 43%, 45%, etc., and other specific values within the above numerical ranges may be selected, so that no further description will be given here.
The 5-7BV may be, for example, 5BV, 6BV, 7BV, etc., and other specific point values within the numerical range may be selected, which will not be described in detail herein.
Preferably, the flocculation treatment uses a flocculant, the preparation raw materials of which comprise chitosan and ZTC1+1 clarifier.
The mass ratio of the chitosan to the ZTC1+1 clarifying agent is (3-1): 1, such as 3:1, 2:1, 1:1, etc., and other specific point values in the numerical range can be selected, and will not be described in detail herein.
It has also been unexpectedly found that it is preferable to use a flocculant composed of chitosan and ZTC1+1 in a mass ratio of (3-1): 1, the relative amounts of saponin and polysaccharide active ingredients contained in the flocculated product are significantly increased, and the extract has more excellent moisturizing and repairing effects.
Preferably, the flocculant is made by a process comprising: mixing the raw materials with 0.5-2% acetic acid aqueous solution, stirring to paste, and swelling with water for more than 20 hr.
The amount of the aqueous acetic acid solution to be used is small relative to the water to be used later, and the concentration thereof may be 0.5%, 0.7%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, etc. Swelling for 20h or more may be 22h, 23h, 24h, 25h, 27h, 28h, 30h, 32h, etc.
The flocculant is used in an amount of 1-3%, e.g., 1%, 1.2%, 1.5%, 2%, 3% of the concentrated eluate.
Preferably, the flocculation treatment is carried out at 40-60 ℃ for 20-60min.
Preferably, the decolorizing treatment uses a decolorizing agent comprising any one or a combination of at least two of diatomaceous earth, silica gel, or neutral alumina.
The amount of the decoloring agent is 1-3%, such as 1%, 1.2%, 1.5%, 2%, 3% and the like, of the concentrated eluent.
Preferably, the decoloring treatment is performed at 40-60 ℃ for 20-60min.
The flocculation treatment and the decoloring treatment are performed separately or simultaneously.
The step (1) further comprises sieving with 20-60 mesh sieve, such as 25 mesh, 30 mesh, 35 mesh, 40 mesh, 45 mesh, 50 mesh, 55 mesh, 60 mesh, etc.
The drying in step (3) comprises any one of spray drying, freeze drying or vacuum drying.
Other specific point values within the above numerical ranges are all selectable, and will not be described in detail herein.
In a second aspect, the present invention provides a method for preparing a extract of Panax ginseng C.A. Meyer having moisturizing effect.
In a third aspect, the present invention provides the use of a extract of Panax ginseng as described in the second aspect for the preparation of a cosmetic having skin moisturizing and/or skin repairing effects.
Preferably, the amount of the extract of Panax ginseng C.A. Meyer in the cosmetic is 0.01-15%, such as 0.01%, 0.05%, 0.08%, 1%, 1.2%, 1.5%, 2%, 3%, 4%, 5%, 6%, 8%, 10%, 12%, 13%, 15%, etc.
In a fourth aspect, the present invention provides the use of a extract of Panax ginseng in accordance with the second aspect for the preparation of an aquaporin-3 expression promoter.
According to the results of the present invention, it is found that the extract of Panax ginseng C.A. Mey prepared by the above specific method has the effect of promoting the expression of aquaporin-3, and therefore, the extract of Panax ginseng C.A. Mey prepared by the above specific method is useful for preparing aquaporin-3 expression promoter, and plays a role in theoretical research on the physiological mechanism of action system of aquaporin-3.
In a fifth aspect, the present invention provides the use of a extract of Panax ginseng in accordance with the second aspect for the preparation of a product having an effect of promoting healing of epidermal cell scratches.
According to the research result of the present invention, the extract of the Panax ginseng C.A. Mey prepared by the above specific method has the effect of promoting the healing of the scarification of the epidermal cells, and therefore the extract of the Panax ginseng C.A. Mey prepared by the above specific method can also be used for promoting the healing of the scarification of the epidermal cells in the related scientific research.
Compared with the prior art, the invention has the following beneficial effects:
the preparation method of the extract of the Panax ginseng C.A. Meyer adopts the modes of ethanol hot extraction, macroporous resin purification and enrichment and flocculation treatment and decoloration treatment creatively, so that the prepared extract has the advantages of light color, less impurities and high extraction efficiency of effective active ingredients (mainly ginsenoside R0, ginsenoside IVa and polysaccharide); on the other hand, the obtained extract also has more excellent effect of promoting the expression of aquaporin-3 in epidermal cells and more excellent effect of promoting epidermal repair, so that the extract of the Panax ginseng C.A. Meyer prepared by the preparation method can be applied to cosmetics with skin moisturizing or skin repairing effects.
Drawings
FIG. 1 is a liquid chromatogram of ginsenoside R0 standard;
FIG. 2 is a liquid chromatogram of a ginsenoside IVa standard;
FIG. 3 is a liquid chromatogram of the extract of Panax ginseng C.A.Meyer prepared in example 1;
FIG. 4 is a graph showing immunofluorescence detection of the expression level of aquaporin-3 in each group;
FIG. 5 is a view of the microscope for cell scratch repair test of each group.
Detailed Description
The technical scheme of the invention is further described by the following specific embodiments. It will be apparent to those skilled in the art that the examples are merely to aid in understanding the invention and are not to be construed as a specific limitation thereof.
The following examples relate to the purchase of Panax ginseng C.A.Meyer from Lijiang lifting agricultural development Co.
The chitosan is a product with the model number of S11064-500g purchased from the biological science and technology Co.Ltd; ZTC1+1 was purchased from the henan hundred flavor food ingredient business.
The HaCaT cells of the following test examples were derived from the Shanghai cell bank of the national academy of sciences.
The yield calculation formula of the extract of the rhizoma panacis majoris is as follows: yield = (M1/M2) ×100%, M1 is weight of the extract of the radix sophorae flavescentis, and M2 is weight of the medicinal material of the radix sophorae flavescentis.
The detection of the contents of ginsenoside R0 and ginsenoside IVa is carried out by adopting a liquid phase method, and specifically comprises the following steps:
ginsenoside R0 and ginsenoside IVa standard are purchased from Shanghai Yuan leaf Biotechnology Co., ltd, detection conditions are referred to in China pharmacopoeia of 2020 edition, octadecyl bonded silica gel is used as filler, acetonitrile-0.2% phosphoric acid water (65:35) is used as mobile phase, flow rate is 1mL/min, detection wavelength is 203nm, column temperature is 30 ℃, and theoretical plate number is not lower than 3000 calculated according to the peak of the ginsenoside IVa. Wherein the liquid chromatograms of the ginsenoside R0 standard and the ginsenoside IVa standard are respectively shown in FIG. 1 and FIG. 2, and the liquid chromatograms of the Panax ginseng extract prepared in example 1 are shown in FIG. 3.
The polysaccharide content detection method comprises the following steps:
(1) Preparing a standard solution: preparing glucose standard solution of 0.1mg/mL, sequentially sucking 0, 0.1, 0.2, 0.4, 0.8, 1.6 and 2mL into test tube, and adding ddH 2 O was made up to 2mL in each tube for use. Preparing a sample solution: accurately weighing the sample powder, adding ddH 2 O is prepared into a sample solution of 0.1mg/mL to be measured. Preparation of 5% phenol solution: 2.5g of phenol solid powder was accurately weighed and 47.5mL of ddH was added 2 O is dissolved at 75 ℃ and stored in a dark place.
(2) Reaction of standard solution and sample solution: sequentially sucking 1mL of the prepared standard solution and sample solution, adding into a test tube, adding 0.5mL of 5% phenol solution and 2.5mL of concentrated sulfuric acid solution for reaction, and gently shaking to obtain ddH 2 O is a blank control, and the absorbance is measured at 490nm by an enzyme-labeled instrument. Drawing a standard curve with the concentration as an abscissa and the absorbance as an ordinate, and obtaining a y value＝0.0049x+0.0018(R 2 =0.9984). The absorbance of the sample solution was substituted into the standard curve, and calculation was performed.
Example 1
The embodiment provides a preparation method of a ginseng extract:
(1) Pulverizing the raw materials of the rhizoma panacis majoris, sieving with a 40-mesh sieve, taking 100g of the crushed raw materials of the rhizoma panacis majoris, carrying out heating reflux extraction by adopting 70% ethanol water solution with the mass of 10 times, extracting twice for 2 hours each time, combining extracting solutions, filtering, and concentrating under reduced pressure to obtain the crude extract of the rhizoma panacis majoris;
(2) Weighing 300g of HP2MGL macroporous resin, loading the macroporous resin into a column by adopting pure water, dissolving the crude extract of the rhizoma panacis majoris in 10 times of water, loading the sample, eluting the sample by using 40% ethanol water solution for 6BV, and collecting eluent;
(3) Concentrating the mixed solution until no alcohol smell exists, adding a flocculating agent with the mass ratio of 3 into the mixed solution, wherein the flocculating agent is 2.5% of the solution, and diatomite with the decoloring agent with the mass ratio of 2.5% of the solution, and stirring for 30min at 50 ℃:1, firstly mixing chitosan and ZTC1+1 with 5 times of 1% acetic acid aqueous solution by mass, stirring to paste, and then mixing with 100 times of water by mass for swelling for 24 hours to obtain the chitosan-ZTC1;
(4) And then standing and cooling, filtering, and freeze-drying to obtain the extract of the rhizoma panacis majoris.
Example 2
The embodiment provides a preparation method of a ginseng extract:
(1) Pulverizing the raw materials of the rhizoma panacis majoris, sieving with a 40-mesh sieve, taking 100g of the raw materials of the rhizoma panacis majoris, carrying out heating reflux extraction by adopting 60% ethanol water solution with the mass of 10 times, extracting twice for 3 hours each time, combining the extracting solutions, filtering, and concentrating under reduced pressure to obtain the crude extract of the rhizoma panacis majoris;
(2) Weighing 300g X-5 macroporous resin, loading the macroporous resin into a column by adopting pure water, dissolving the crude extract of the rhizoma panacis majoris in 10 times of water by mass for loading, eluting with 10% ethanol water solution for 7BV, eluting front impurities, eluting with 35% ethanol water solution for 7BV, and collecting eluent;
(3) Concentrating the eluent until no alcohol smell exists, adding a flocculating agent with the mass ratio of 1% and a decoloring agent with the mass ratio of 1:1, namely, mixing and stirring chitosan with ZTC1+1 and 1% of acetic acid aqueous solution with the mass ratio of 5 times to form paste, and then mixing and swelling with 100 times of water for 24 hours to obtain the eluent;
(4) And then standing and cooling, filtering, and freeze-drying to obtain the extract of the rhizoma panacis majoris.
Example 3
The embodiment provides a preparation method of a ginseng extract:
(1) Pulverizing rhizoma Panacis Quinquefolii, sieving with 40 mesh sieve, heating and reflux extracting 100g with 80% ethanol water solution 10 times of the total mass for 2 hr for two times, mixing extractive solutions, filtering, concentrating under reduced pressure until no ethanol smell exists to obtain crude extract of rhizoma Panacis Quinquefolii;
(2) 300g of the SP850 macroporous resin is weighed, pure water is adopted for loading, the crude extract of the pearl ginseng is dissolved in 10 times of water for loading, the pure water is used for eluting 5BV, the impurities at the front end are eluted, then the 45% ethanol water solution is used for eluting 5BV, and the eluent is collected;
(3) Concentrating the eluent until no alcohol smell exists, adding a flocculating agent with the mass ratio of 1.5% and a decoloring agent neutral alumina with the mass ratio of 1.5% into the solution, stirring for 30min at 50 ℃, wherein the flocculating agent is obtained by mixing chitosan and ZTC1+1 with the mass ratio of 2:1, stirring into paste with a 1% acetic acid aqueous solution with the mass ratio of 5 times, and then mixing and swelling with water with the mass ratio of 100 times for 24 h;
(4) And then standing and cooling, filtering, and freeze-drying to obtain the extract of the rhizoma panacis majoris.
Example 4
This example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that the HP2MGL macroporous resin is replaced by D101 macroporous resin in equal amounts, and the other conditions are kept unchanged.
Example 5
This example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that the HP2MGL macroporous resin is replaced with AB-8 macroporous resin in equal amounts, and the other conditions remain unchanged.
Example 6
This example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that the elution process of step (2) is: eluting with 20% ethanol water solution for 6BV, eluting front-end impurities, eluting with 40% ethanol water solution for 6BV, collecting eluate, and keeping other conditions unchanged.
Example 7
This example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that the elution process of step (2) is: the front-end impurities were not eluted, then the eluate was collected by eluting with 25% aqueous ethanol for 6BV, and the other conditions were kept unchanged.
Example 8
This example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that the flocculant is a single chitosan, but the amount of chitosan used is still 2.5%, and the preparation method is unchanged.
Example 9
This example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that a single ZTC1+1 is used as the flocculant, but the amount of the flocculant is still 2.5%, and the preparation method is unchanged.
Comparative example 1
This comparative example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that the treatment is performed without adding a flocculant, and the other conditions remain unchanged.
Comparative example 2
This comparative example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that the treatment is performed without adding a decoloring agent, and other conditions are maintained unchanged. The color of the product was significantly darker than that of example 1.
Comparative example 3
This comparative example provides a method for preparing a extract of Panax ginseng, which differs from example 1 only in that the treatment is performed without adding flocculant and decolorant, and other conditions are maintained unchanged. The color of the product was significantly darker than that of example 1.
Test example 1
The extracts of Panax ginseng C.A. Meyer prepared in examples 1-9 and comparative examples 1-3 were subjected to measurement of ginsenoside R0, ginsenoside IVa content, and polysaccharide content, and the results are shown in Table 1:
TABLE 1
As can be seen from the data in Table 1, the preparation method of the extract of Panax ginseng of the present invention can not only make the concentration ratio of ginsenoside R0 and ginsenoside IVa in the extract be at a high level, but also make the ratio of ginsenoside R0, ginsenoside IVa and total polysaccharide in the extract be within a reasonable interval range, so that the extract can play a remarkable role in repairing and moisturizing; the type of macroporous resin and the elution procedure of macroporous resin, especially flocculation treatment and decolorization treatment, influence the concentration ratio of ginsenoside R0, ginsenoside IVa and total polysaccharide in the extract and the balance between the ginsenoside IVa and the total polysaccharide in the extract, and influence the efficacy of the extract of the Panax ginseng C.A. Meyer in the aspects of moisture preservation and repair.
Test example 2
Determination of moisturizing and repair Activity on the extracts of Panax ginseng C.A. Meyer prepared in examples 1,6-7 and comparative examples 1-3:
(1) Moisture efficacy activity assay:
aquaporin-3 plays an important role in maintaining skin moisture and skin function, an experiment adopts an immunofluorescence method to measure the influence of bletilla striata extract on the expression quantity of the aquaporin-3, haCaT cells are inoculated into a 24-well plate, and a normal control group and a positive control group (CaCl) are arranged in the experiment 2 3 mM) and the resultant extract of Panax schinseng (120. Mu.g/mL) in an incubator (37 ℃ C., 5% CO) 2 ) Incubating for 12h, when the plating rate reaches 40% -60%, carrying out grouped administration, incubating for 24h in an incubator after the administration is completed, fixing cells with 4% paraformaldehyde after the incubation is completed, carrying out immunofluorescence detection after 24h, photographing by adopting a fluorescence microscope, and using an Image-Image processingThe software performs the analysis. The test results are shown in table 2:
TABLE 2
As can be seen from the data in table 2, the extract of the panaxacum prepared by the specific extraction process of the present invention has remarkably excellent effect of promoting the expression of aquaporin-3, i.e., moisturizing effect. At the same time, flocculation treatment and decoloration treatment significantly affect the promotion effect on aquaporin-3 expression. And the results of comparative example 1 and examples 6 to 7 show that the extract can exert remarkable effects on moisture preservation by making the ratio of ginsenoside R0, ginsenoside IVa and total polysaccharide content in the extract within a reasonable interval range.
(2) Determination of scratch repair Activity:
placing cell scratch inserts vertically in the middle of each 12-hole cell culture plate, respectively adding HaCaT cell suspension and CO with 5% concentration into two chambers of each insert 2 Culturing at 37deg.C for 24 hr. The inserts were gently removed with sterile forceps, the cells were gently rinsed one to two times with 1mL PBS solution and all PBS was aspirated off. The cell growth status was observed under a microscope for 0 hours, 16 hours, 24 hours and 48 hours at scratches in the normal control group (serum-free DMEM medium) and the positive control group (epidermal growth factor EGF solution concentration: 10000 IU/mL), and the subject-treated group (bead ginseng extract 120 μg/mL extracted from different groups), and the healing area was calculated using ImageJ software, as shown in fig. 5, by photographing the recordings, respectively. The healing rate calculation formula is as follows: healing rate = (initial scratch area-scratch area to be measured time)/initial scratch area. The test results are shown in table 3:
TABLE 3 Table 3
As can be seen from the data in table 3, the extract of the panaxacum prepared by the specific extraction process of the present invention has remarkably excellent efficacy of promoting healing and repairing of epidermal cells. At the same time, flocculation treatment and decoloration treatment obviously influence the efficacy of promoting the healing and repairing of the epidermal cells. And the results of comparative example 1 and examples 6 to 7 show that the extract can exert remarkable effects in repairing by making the ratio of ginsenoside R0, ginsenoside IVa and total polysaccharide content in the extract within a reasonable interval range.
Test example 3
The skin irritation and eye irritation of the extracts of Panax ginseng C.A.Meyer prepared in examples 1 to 3 were measured according to cosmetic safety Specification 2015, and the results are shown in Table 4.
TABLE 4 Table 4
The results show that the prepared rhizoma panacis majoris extract has no skin irritation and eye irritation and has good safety.
The applicant states that the present invention is described by way of the above examples as a method of preparing a extract of Panax ginseng, and products and applications thereof, but the present invention is not limited to the above examples, i.e., it is not meant that the present invention must be practiced in dependence upon the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (10)
1. A preparation method of a ginseng extract with repairing and moisturizing effects, which is characterized by comprising the following steps:
(1) Pulverizing rhizoma Panacis Quinquefolii, extracting with 60-80% ethanol water solution under reflux, filtering the extractive solution, and concentrating to obtain crude extract;
(2) Purifying and eluting the crude extract of the rhizoma panacis majoris by macroporous resin, and collecting eluent;
(3) Concentrating the eluent, performing flocculation treatment and decoloration treatment, and then drying to obtain the extract of the rhizoma panacis majoris with the effects of repairing and moisturizing.
2. The method for preparing a extract of Panax ginseng C.A. Meyer with repairing and moisturizing effects according to claim 1, wherein the macroporous resin comprises any one of SP850, HP2MGL or X-5.
3. The method for preparing the extract of the panaxacum with repairing and moisturizing effects according to claim 1, wherein the purification and elution process is as follows: eluting front-end impurities or non-eluting front-end impurities with 5-7BV 0-10% ethanol water solution, eluting 5-7BV with 35-45% ethanol water solution, and collecting the eluate.
4. The method for preparing a ginseng extract with repairing and moisturizing effects according to claim 1, wherein the flocculation treatment uses a flocculant, and the preparation raw materials of the flocculant comprise chitosan and a ZTC1+1 clarifier;
the mass ratio of the chitosan to the ZTC1+1 clarifying agent is (3-1): 1.
5. The method for preparing a extract of Panax ginseng C.A. Meyer having moisturizing effect as claimed in claim 4, wherein the flocculant is prepared by a method comprising: mixing the preparation raw materials with 0.5-2% acetic acid aqueous solution, stirring to paste, and swelling with water for more than 20 hr;
the dosage of the flocculant is 1-3% of the concentrated eluent.
6. The method for preparing a extract of Panax ginseng C.A. Meyer having moisturizing effect for repairing according to claim 1, wherein the decolorizing treatment comprises a decolorizing agent comprising one or a combination of at least two of diatomaceous earth, silica gel or neutral alumina;
the dosage of the decoloring agent is 1-3% of the concentrated eluent;
the flocculation treatment and the decoloring treatment are respectively or simultaneously carried out;
the crushed materials in the step (1) are further sieved by a 20-60 mesh sieve;
the drying in step (3) comprises any one of spray drying, freeze drying or vacuum drying.
7. The extract of Panax ginseng C.A. Meyer having moisturizing effect as claimed in any one of claims 1 to 6.
8. Use of the extract of Panax ginseng C.A. Meyer of claim 7 for preparing cosmetics with skin moisturizing and/or skin repairing effects.
9. Use of a extract of a Panax ginseng wall according to claim 7 for the preparation of an aquaporin-3 expression promoter.
10. Use of the extract of Panax ginseng C.A. Meyer of claim 7 for preparing a product having effect of promoting healing of epidermal cell scratch.
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