CN116919847A - Cosmetic rich in cell active factors and application thereof - Google Patents
Cosmetic rich in cell active factors and application thereof Download PDFInfo
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- CN116919847A CN116919847A CN202311112806.5A CN202311112806A CN116919847A CN 116919847 A CN116919847 A CN 116919847A CN 202311112806 A CN202311112806 A CN 202311112806A CN 116919847 A CN116919847 A CN 116919847A
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- chitosan
- sodium polyphosphate
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- spirulina
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- 235000016425 Arthrospira platensis Nutrition 0.000 claims abstract description 65
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 65
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- 229920001661 Chitosan Polymers 0.000 claims abstract description 64
- 229920000388 Polyphosphate Polymers 0.000 claims abstract description 42
- 239000001205 polyphosphate Substances 0.000 claims abstract description 42
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- 229910052708 sodium Inorganic materials 0.000 claims abstract description 42
- 239000011734 sodium Substances 0.000 claims abstract description 42
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 38
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- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 claims abstract description 14
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- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 claims abstract description 14
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 claims abstract description 14
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 13
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/24—Phosphorous; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Emergency Medicine (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a cosmetic rich in cell active factors and application thereof. Cosmetics rich in cytokines include glycerol, ceramide, erythritol, trehalose, and cytokines; the cell active factor is spirulina polypeptide-sodium polyphosphate chitosan nanoparticle; the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle is prepared by crosslinking spirulina polypeptide and sodium polyphosphate chitosan nanoparticle ions. After the spirulina polypeptide is crosslinked with the sodium polyphosphate chitosan, the invention has good biocompatibility, degradability, good film forming property and high antibacterial activity, and greatly enhances the absorption capacity of human bodies and the oxidation resistance of skin. Because the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle has good film forming property, when the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle is used on the surface of human skin, an antioxidant film layer can be formed, and daily damage of the skin is reduced.
Description
Technical Field
The invention relates to the field of cosmetics, in particular to a cosmetic rich in cell active factors and application thereof.
Background
Cytokines are polypeptides that bind to cell membrane receptors and regulate cell growth or other cellular functions. Cell culture requires the synergistic effect of multiple growth factors. Most of the cell active factors are bioactive peptides, and are composed of 2-20 amino acids, the molecular weight is relatively small, and the functional activity is determined by the amino acid sequence and the structural characteristics.
The cell active factors added in the existing cosmetics are mainly cell repair and cell nutrition supplement, but the cosmetics are external application products, so that the absorption of the cell active factors is low after the skin is coated, the inactivation speed is relatively high, and the repair effect is poor.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a cosmetic rich in the cell active factors, which can keep the activity of the cell active factors for a long time without inactivation and has better absorption rate and repair function, and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a cosmetic rich in cell active factors comprises glycerol, ceramide, erythritol, trehalose, and cell active factors; the cell active factor is spirulina polypeptide-sodium polyphosphate chitosan nanoparticle; the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle is prepared by crosslinking spirulina polypeptide and sodium polyphosphate chitosan nanoparticle ions.
The invention also provides the following preferable scheme:
preferably, the components comprise, by mass, 2-4 parts of glycerol, 1-5 parts of ceramide, 1-4 parts of erythritol, 1-2 parts of trehalose and 10-23 parts of spirulina polypeptide-sodium polyphosphate chitosan nanoparticles.
More preferably, the components comprise, by mass, 3 parts of glycerol, 3 parts of ceramide, 2 parts of erythritol, 2 parts of trehalose and 23 parts of spirulina polypeptide-sodium polyphosphate chitosan nanoparticle.
Preferably, the preparation method of the spirulina polypeptide comprises the following steps: pulverizing a certain amount of spirulina, adding protease to perform enzymolysis on spirulina, centrifuging to obtain spirulina polypeptide enzymolysis solution, and spray drying to obtain spirulina polypeptide.
The enzymolysis time is preferably 2-4 hours, and the enzymolysis is performed after enzyme inactivation and then centrifugation.
Preferably, the protease is proteinase K. The protease may be proteinase K, trypsin, pepsin, papain, etc.
Preferably, the preparation method of the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle comprises the following steps: dissolving chitosan in an acidic solution, centrifuging to remove impurities, dropwise adding a sodium polyphosphate solution into the chitosan solution, continuously stirring in the dropwise adding process to obtain the sodium chitosan polyphosphate solution, uniformly mixing the sodium chitosan polyphosphate solution with the spirulina polypeptide solution, dropwise adding sodium polyphosphate into the solution in the process, stirring, and reacting to obtain the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle.
Preferably, the acidic solution is one or more of phosphoric acid, citric acid and acetic acid.
When chitosan is dissolved in acid solution, its concentration is kept at 1-5mg/mL, and its rotation speed is selected at 2000-4000rpm when it is centrifuged.
Preferably, the concentration of the sodium polyphosphate solution is 0.1-1.0mg/mL.
Preferably, the pH value of chitosan dissolved in the acid solution is controlled to be 4.0-5.6.
The invention also discloses application of the cosmetics rich in the cell active factors, which is mainly applied to preparation of cosmetics.
The invention has the beneficial effects that:
the spirulina polypeptide has low molecular weight and good physicochemical characteristics, is easy to be absorbed by human body, has good antioxidation function, can reduce the damage of free radicals of human body, has good immunoregulatory capability, but has poor absorbability when being used independently. The spirulina polypeptide-sodium polyphosphate chitosan nanoparticle is adopted, and after the spirulina polypeptide and the sodium polyphosphate chitosan are crosslinked, the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle has good biocompatibility, degradability, good film forming property and high antibacterial activity, and the absorption capacity of a human body and the oxidation resistance of skin are greatly enhanced. Because the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle has good film forming property, when the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle is used on the surface of human skin, an antioxidant film layer can be formed, and daily damage of the skin is reduced.
Drawings
FIG. 1 is a chart showing staining of skin tissue sections of example 5 in a skin aging test;
FIG. 2 is a chart showing staining of skin tissue sections of example 4 in a skin aging test;
FIG. 3 is a chart showing staining of skin tissue sections of comparative examples in skin aging experiments.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments thereof in order to enable those skilled in the art to better understand the technical aspects of the invention.
The cosmetics rich in the cell active factors comprise glycerol, ceramide, erythritol, trehalose and the cell active factors; the cell active factor is spirulina polypeptide-sodium polyphosphate chitosan nanoparticle; the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle is prepared by crosslinking spirulina polypeptide and sodium polyphosphate chitosan nanoparticle ions.
The glycerol is used as a humectant, and has the effects and effects of moisturizing, locking water, nourishing, promoting damaged cell repair and the like. Ceramide exists in eukaryotic cells and has important regulation effects on vital activities such as cell differentiation, proliferation, apoptosis, aging and the like. The ceramide is used as a main component of intercellular lipid of skin horny layer, is used as a second messenger molecule in a sphingomyelin pathway, plays an important role in the formation process of the skin horny layer, and has the effects of maintaining skin barrier, moisturizing, resisting aging, whitening, treating diseases and the like. Erythritol has strong antioxidation effect, can resist free radical injury, protect skin from external factors such as ultraviolet radiation and environmental pollution, and thus slow down skin aging. Trehalose can remove free radicals generated by alpha rays and beta rays, so that cell DNA is not easy to mutate. Can effectively protect DNA from damage caused by radioactive rays, and is a protective repair factor of skin cell DNA.
The invention is mainly aimed at improving the oxidation resistance of skin, moisturizing by using glycerol, repairing skin by using ceramide and protecting radioactive rays by using erythritol and trehalose. Because the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle is used for constructing the skin protection layer, the invention has good film forming property, greatly improves the absorption rate of polypeptide and nutrient components, can penetrate into the cortex, and greatly increases the oxidation resistance of the skin.
The invention relates to a cosmetic rich in cell active factors, which comprises, by mass, 2-4 parts of glycerol, 1-5 parts of ceramide, 1-4 parts of erythritol, 1-2 parts of trehalose and 10-23 parts of spirulina polypeptide-sodium polyphosphate chitosan nanoparticles.
In a preferred embodiment, the components include, by mass, 3 parts of glycerol, 3 parts of ceramide, 2 parts of erythritol, 2 parts of trehalose, and 23 parts of spirulina polypeptide-sodium polyphosphate chitosan nanoparticle.
The preparation method of the spirulina polypeptide comprises the following steps: pulverizing a certain amount of spirulina, adding protease to perform enzymolysis on spirulina, centrifuging to obtain spirulina polypeptide enzymolysis solution, and spray drying to obtain spirulina polypeptide.
The protease is proteinase K. The protease may be proteinase K, trypsin, pepsin, papain, etc.
The preparation method of the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle comprises the following steps: dissolving chitosan in an acidic solution, centrifuging to remove impurities, dropwise adding a sodium polyphosphate solution into the chitosan solution, continuously stirring in the dropwise adding process to obtain the sodium chitosan polyphosphate solution, uniformly mixing the sodium chitosan polyphosphate solution with the spirulina polypeptide solution, dropwise adding sodium polyphosphate into the solution in the process, stirring, and reacting to obtain the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle.
The acidic solution is one or more of phosphoric acid, citric acid and acetic acid.
When chitosan is dissolved in acid solution, its concentration is kept at 1-5mg/mL, and its rotation speed is selected at 2000-4000rpm when it is centrifuged.
The concentration of the sodium polyphosphate solution is 0.1-1.0mg/mL.
The pH value of chitosan dissolved in acid solution is controlled to be 4.0-5.6.
The invention also discloses application of the cosmetics rich in the cell active factors, which is mainly applied to preparation of cosmetics.
The present invention is described above as an outline of the present invention, and the present invention is further described below with reference to examples.
1. Preparation of spirulina polypeptide
Pulverizing 50g of spirulina by ultrasonic waves, placing into 5000mL of deionized water after pulverizing, uniformly mixing, preserving heat at 80-95 ℃ for 10-20min, cooling to 50-65 ℃, adding protease for hydrolysis, and keeping the enzyme addition amount at 4000-4700U/g or 3-5% according to the mass ratio of a substrate, wherein the enzymolysis time is kept for 2-4 hours. Inactivating enzyme in boiling water bath for 10-20min after hydrolysis, cooling to room temperature, centrifuging to obtain spirulina polypeptide enzymolysis solution, and spray drying to obtain spirulina polypeptide.
2. Preparation of spirulina polypeptide-sodium polyphosphate chitosan nanoparticle
And dissolving chitosan in an acidic solution at room temperature, wherein the acidic solution can be one of phosphoric acid, citric acid and acetic acid, the concentration of the acidic solution is kept at 2-5%, the pH value is mainly adjusted to be 4.0-5.6, the concentration of the chitosan is kept at 1-5mg/mL, and the dissolution rate is kept under the condition that the solubility of the chitosan is high. After chitosan is dissolved, the solution is placed in a centrifuge and centrifuged at 2000-4000rpm to obtain supernatant.
Dropwise adding a sodium tripolyphosphate solution with the concentration of 0.1-1mg/mL into a chitosan solution, continuously magnetically stirring in the dropwise adding process to obtain a sodium chitosan polyphosphate solution, uniformly mixing the sodium chitosan polyphosphate solution (1-5 mg/mL) and a spirulina polypeptide solution (0.5-2.0 mg/mL), dropwise adding the sodium tripolyphosphate solution with the concentration of 0.1-1mg/mL into the mixed solution, magnetically stirring for 1-2 hours to obtain a spirulina polypeptide-sodium polyphosphate chitosan nanoparticle solution, and removing the solution by an evaporating instrument to obtain spirulina polypeptide-sodium polyphosphate chitosan nanoparticles.
3. Preparation of cosmetics rich in cell active factor
Mixing glycerol 2-4 parts, ceramide 1-5 parts, erythritol 1-4 parts, trehalose 1-2 parts, spirulina polypeptide-sodium polyphosphate chitosan nanoparticle 10-23 parts by weight, and adding corresponding auxiliary materials or solvents to obtain the cosmetics rich in cell active factors.
Experimental detection
1-1 measurement of hydrolysis degree
And detecting the hydrolysis degree of the hydrolysate by adopting a formaldehyde titration method.
Taking 10mL of the hydrolyzed solution and the unhydrolyzed raw material solution after enzyme deactivation, placing the hydrolyzed solution and the unhydrolyzed raw material solution into a volumetric flask with 100mL, adding pure water into the volumetric flask with 100mL, and uniformly mixing. 100mL of solution was prepared by pipetting 20mL to 80mL of water, titrating with a standard solution of sodium hydroxide at a concentration of 0.05mol/L, and recording the volume of sodium hydroxide solution consumed. Each sample was measured in triplicate.
1-2, hydrolysis condition selection
The temperature and the ratio of enzyme to substrate are selected as influencing factors, and the orthogonal experimental configuration is carried out according to the following table.
| Numbering device | Temperature (temperature) | Enzyme to substrate ratio |
| 1 | 50 | 3％ |
| 2 | 55 | 4％ |
| 3 | 65 | 5％ |
TABLE 1 orthogonal experiment parameter control Table
Hydrolysis orthogonal experiments were performed on proteinase K according to the hydrolysis conditions of Table 1 and the results of Table 2 were obtained
| Numbering device | Temperature (temperature) | Enzyme to substrate ratio | Degree of hydrolysis (%) |
| 1 | 50 | 3％ | 75.4 |
| 2 | 50 | 4％ | 78.3 |
| 3 | 50 | 5％ | 81.7 |
| 4 | 55 | 3％ | 83.6 |
| 5 | 55 | 4％ | 86.9 |
| 6 | 55 | 5％ | 88.3 |
| 7 | 65 | 3％ | 71.6 |
| 8 | 65 | 4％ | 73.8 |
| 9 | 65 | 5％ | 77.2 |
TABLE 2 results of orthogonal proteinase K experiments
As can be seen from Table 2, proteinase K has a relatively low enzymatic hydrolysis efficiency at 50℃and is easily inactivated at a high temperature at 65℃and is relatively ideal at 55℃with a 5% rate being the highest.
1-3, spirulina polypeptide-sodium polyphosphate chitosan nanoparticle preparation condition control
Controlling technological parameters under different experimental conditions and detecting the particle size of the obtained nano particles to obtain a table 3
TABLE 3 Process parameters table and particle size of spirulina polypeptide-sodium polyphosphate chitosan nanoparticle
The spirulina polypeptide-sodium polyphosphate chitosan nanoparticle in table 3 was subjected to particle size detection to obtain the data in table 3, and the size of the nanoparticle which can be absorbed by human body was below 130 nm, so that only experiments 1-3 can meet specific human body absorption requirements. The experimental parameter procedure of number 2 was chosen.
1-4 antioxidant Property test of cosmetics containing cytokine
The components of the cosmetics rich in the cell active factors are proportioned according to the following table 4
TABLE 4 formulation of cosmetic compositions enriched in cytokine
The cosmetics rich in the cytokine in each component proportion were added into an aqueous solution to be sufficiently dissolved, and DPPH free radical scavenging ability was measured by examples 1 to 5 and comparative example, to obtain Table 5.
| DPPH radical scavenging ability (%) | |
| Example 1 | 70.6 |
| Example 2 | 73.5 |
| Example 3 | 76.3 |
| Example 4 | 78.6 |
| Example 5 | 83.5 |
| Comparative example | 74.6 |
TABLE 5DPPH free radical scavenging Capacity Table
From Table 5, it is clear that DPPH radical scavenging ability is mainly due to the influence of the content of spirulina polypeptide.
Anti-aging test of mouse skin cells as in examples 4, 5 and comparative example
Mice were grouped, three in each group, and cosmetics rich in cytokines of example 4, example 5, and comparative example 1 were added to 100mL of an aqueous solution to be dissolved, back hairs of the mice were removed, then D-gal was performed on the mice to induce cell senescence, and outer skins thereof were coated 2 times per day, and skin tissues of the mice were sectioned and stained after continuous coating for 6 weeks, to obtain the section charts of fig. 1 to 3. FIG. 1 is a staining chart of a skin tissue section of example 5, from which it can be seen that the dermis layer has a high collagen fiber content, large fibers, and clear boundaries without obvious fracture marks. Fig. 2 is a staining chart of a skin tissue section of example 4, from which it can be seen that the dermis layer has a relatively low collagen fiber content, significantly lower than that of example 5, and has a partial trace of fracture at the boundary. FIG. 3 is a chart of staining of skin tissue sections of comparative example, from which it can be seen that the dermis layer has a small collagen fiber content and a large and distinct trace of boundary fracture. The cosmetic rich in the cell active factors has obvious inhibiting effect on skin aging.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that the above-mentioned preferred embodiment should not be construed as limiting the invention, and the scope of the invention should be defined by the appended claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and such modifications and adaptations are intended to be comprehended within the scope of the invention.
Claims (10)
1. A cosmetic enriched in cytokines, characterized in that: including glycerol, ceramide, erythritol, trehalose, and cytokines; the cell active factor is spirulina polypeptide-sodium polyphosphate chitosan nanoparticle; the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle is prepared by crosslinking spirulina polypeptide and sodium polyphosphate chitosan nanoparticle ions.
2. The cytokine-rich cosmetic of claim 1, wherein: the components comprise, by mass, 2-4 parts of glycerol, 1-5 parts of ceramide, 1-4 parts of erythritol, 1-2 parts of trehalose, and 10-23 parts of spirulina polypeptide-sodium polyphosphate chitosan nanoparticles.
3. The cytokine-rich cosmetic of claim 2, wherein: the method is characterized in that: the components comprise 3 parts by weight of glycerin, 3 parts by weight of ceramide, 2 parts by weight of erythritol, 2 parts by weight of trehalose and 23 parts by weight of spirulina polypeptide-sodium polyphosphate chitosan nanoparticle.
4. The cytokine-rich cosmetic of claim 1, wherein: the preparation method of the spirulina polypeptide comprises the following steps: pulverizing a certain amount of spirulina, adding protease to perform enzymolysis on spirulina, centrifuging to obtain spirulina polypeptide enzymolysis solution, and spray drying to obtain spirulina polypeptide.
5. The cytokine-rich cosmetic of claim 4, wherein: the protease is proteinase K.
6. The cytokine-rich cosmetic of claim 1, wherein: the preparation method of the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle comprises the following steps: dissolving chitosan in an acidic solution, centrifuging to remove impurities, dropwise adding a sodium polyphosphate solution into the chitosan solution, continuously stirring in the dropwise adding process to obtain the sodium chitosan polyphosphate solution, uniformly mixing the sodium chitosan polyphosphate solution with the spirulina polypeptide solution, dropwise adding sodium polyphosphate into the solution in the process, stirring, and reacting to obtain the spirulina polypeptide-sodium polyphosphate chitosan nanoparticle.
7. The cytokine-rich cosmetic of claim 6, wherein: the acidic solution is one or more of phosphoric acid, citric acid and acetic acid.
8. The cytokine-rich cosmetic of claim 6, wherein: the concentration of the sodium polyphosphate solution is 0.1-1mg/mL.
9. The cytokine-rich cosmetic of claim 6, wherein: the pH value of chitosan dissolved in acid solution is controlled to be 4.0-5.6.
10. Use of a cosmetic enriched in cytokines according to claim 1, characterized in that: the cosmetics rich in the cytokines are applied to the preparation of the cosmetics.
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| CN102846553A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella polypeptide-chitosan nanoparticle |
| CN103110585A (en) * | 2012-08-31 | 2013-05-22 | 华南理工大学 | Method for preparing spirulina polypeptide-chitosan nano particles |
| CN112335796A (en) * | 2020-11-06 | 2021-02-09 | 清远希普生物科技有限公司 | Composition containing spirulina polypeptide and application thereof |
| CN114306204A (en) * | 2022-01-04 | 2022-04-12 | 内蒙古科技大学 | Pure natural spirulina polypeptide mask and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102846553A (en) * | 2012-08-31 | 2013-01-02 | 华南理工大学 | Preparation method for chlorella polypeptide-chitosan nanoparticle |
| CN103110585A (en) * | 2012-08-31 | 2013-05-22 | 华南理工大学 | Method for preparing spirulina polypeptide-chitosan nano particles |
| CN112335796A (en) * | 2020-11-06 | 2021-02-09 | 清远希普生物科技有限公司 | Composition containing spirulina polypeptide and application thereof |
| CN114306204A (en) * | 2022-01-04 | 2022-04-12 | 内蒙古科技大学 | Pure natural spirulina polypeptide mask and preparation method thereof |
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