CN116908171A - Color reagent for detecting compost maturity as well as preparation method and application thereof - Google Patents
Color reagent for detecting compost maturity as well as preparation method and application thereof Download PDFInfo
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- CN116908171A CN116908171A CN202310884034.0A CN202310884034A CN116908171A CN 116908171 A CN116908171 A CN 116908171A CN 202310884034 A CN202310884034 A CN 202310884034A CN 116908171 A CN116908171 A CN 116908171A
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- 239000002361 compost Substances 0.000 title claims abstract description 72
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title abstract description 9
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims abstract description 69
- 238000001514 detection method Methods 0.000 claims abstract description 53
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 46
- 235000006408 oxalic acid Nutrition 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 13
- 210000003608 fece Anatomy 0.000 claims description 50
- 239000010871 livestock manure Substances 0.000 claims description 36
- 241000287828 Gallus gallus Species 0.000 claims description 17
- 241001494479 Pecora Species 0.000 claims description 17
- 239000002994 raw material Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 14
- 238000009264 composting Methods 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 238000000855 fermentation Methods 0.000 abstract description 2
- 230000004151 fermentation Effects 0.000 abstract description 2
- 230000018109 developmental process Effects 0.000 description 20
- 238000000354 decomposition reaction Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000007789 gas Substances 0.000 description 7
- 230000035784 germination Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000003895 organic fertilizer Substances 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 239000001272 nitrous oxide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides a color reagent for detecting compost maturity, and a preparation method and application thereof, and relates to the technical field of compost fermentation. Color-developing agent including water, bromocreosote blue, bromophenol blue, oxalic acid and ethanol; the volume ratio of water, the mass of the bromocreosote blue, the mass of oxalic acid and the volume ratio of ethanol is 250ml to 0.5-1 g to 0.8-2 g to 50ml. The color developing agent provided by the invention is used for detecting the compost maturity for 15min to obtain a detection result, and meanwhile, the sensitivity is high, the detection method is simple and convenient, and the conventional and standardized detection of the compost maturity detection can be promoted to a certain extent.
Description
Technical Field
The invention relates to the technical field of compost fermentation, in particular to a color reagent for detecting compost maturity, a preparation method and application thereof.
Background
The degree of decomposition is an index reflecting the degree of stabilization of the composting process. Namely, organic matters in the compost are mineralized and humified, and finally reach a stable state index. The higher the decomposition degree of the compost product is, the less adverse effect is caused to the environment, and the soil fertility can be improved and the plant growth can be promoted. At present, the composting degree of the tested compost products mainly has three indexes, namely: physical, chemical (including humus) and biological indices. However, the indexes have long detection time, high detection cost and high professional quality for practitioners in the composting industry, and the development of the composting industry is limited to a certain extent.
Disclosure of Invention
In order to solve the problems, the invention provides the color reagent for detecting the compost maturity, the preparation method and the application thereof, and the color reagent provided by the invention is adopted to detect the compost maturity for 15min to obtain a detection result, and meanwhile, the sensitivity is high, the detection method is simple and convenient, and the conventional and standardized detection of the compost maturity detection can be promoted to a certain extent.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a color reagent for detecting compost maturity, which comprises water, bromocreosote blue, bromophenol blue, oxalic acid and ethanol;
the volume ratio of water, the mass of the bromocreosote blue, the mass of oxalic acid and the volume ratio of ethanol is 250ml to 0.5-1 g to 0.8-2 g to 50ml.
Preferably, when the raw material of the compost is chicken manure, the volume ratio of water, the mass of bromocreosote blue, the mass of bromophenol blue, the mass of oxalic acid and ethanol is 250ml:1g:1g:2g:50ml.
Preferably, when the raw material of the compost is sheep manure, the volume ratio of the water, the mass of the bromphenol blue, the mass of the oxalic acid and the volume ratio of the ethanol is 250ml:0.7g:0.7g:1.2g:50ml.
Preferably, when the raw material of the compost is cow dung, the volume ratio of the water, the mass of the bromphenol blue, the mass of the oxalic acid and the volume ratio of the ethanol is 250ml to 0.5g to 0.8g to 50ml.
The invention also provides a preparation method of the color developing agent, which comprises the following steps: and dissolving the bromocreosote blue, the bromophenol blue and the oxalic acid in ethanol, and then mixing with water to obtain the color developing agent.
The invention also provides application of the color developing agent in detecting compost maturity.
The invention also provides a kit for detecting the compost maturity, which comprises a detection device, a color card and the color reagent according to the technical scheme;
the detection device comprises a container 1 and a container 2, and n breather holes are formed in the middle of the detection device.
The invention also provides a method for detecting the compost maturity, which comprises the following steps: placing a compost sample into a container 1 of the detection device of the kit according to the technical scheme, placing the color developing agent into a container 2, and comparing the color developing card after 15min to obtain the decomposition degree of the compost sample.
Preferably, the compost sample is placed in an amount of 4g.
Preferably, the amount of the developer to be placed is 0.5ml.
The mechanism for detecting the compost maturity of the invention is as follows: in the composting process, ammonia, carbon dioxide, nitrous oxide and other gases are continuously released, the release amount of the gases is reduced along with the composting, and substances in the color development liquid react with the gases in a combined way to influence the color change. Wherein, the higher the gas concentration, the darker the color of the color liquid, the lower the gas concentration, and the lighter the color of the color liquid.
The beneficial effects of the invention are as follows:
the color developing agent provided by the invention is used for detecting the compost maturity for 15min to obtain a detection result, and meanwhile, the sensitivity is high, the detection method is simple and convenient, and the conventional and standardized detection of the compost maturity detection can be promoted to a certain extent.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments will be briefly described below.
Fig. 1 is a diagram of a detection device, container 1: placing a compost detection sample; container 2: placing a color developing solution; and (3) ventilation holes: ensuring the emission of gas in the material; the container 2 is placed in the container 1, and gas diffuses through the ventilation holes in the container 2;
FIG. 2 is a color chart.
Detailed Description
The invention provides a color reagent for detecting compost maturity, which comprises water, bromocreosote blue, bromophenol blue, oxalic acid and ethanol; the volume ratio of water, the mass of the bromocreosote blue, the mass of oxalic acid and the volume ratio of ethanol is 250ml to 0.5-1 g to 0.8-2 g to 50ml.
In the present invention, when the raw material of the compost is preferably chicken manure, the volume ratio of the water, the mass of the bromphenol blue, the mass of the oxalic acid and the volume ratio of ethanol is preferably 250ml:1g:1g:2g:50ml. The raw materials, the method and the like of the chicken manure compost are not particularly limited, and the chicken manure compost can be used routinely by a person skilled in the art.
In the present invention, when the raw material of the compost is preferably sheep manure, the volume ratio of the water, the mass of the bromocreosote blue, the mass of the bromophenol blue, the mass of the oxalic acid and the volume ratio of ethanol is preferably 250ml:0.7g:0.7g:1.2g:50ml. The raw materials, the method and the like of the sheep manure compost are not particularly limited, and the sheep manure compost can be routinely used by a person skilled in the art.
In the present invention, when the raw material of the compost is preferably cow dung, the volume ratio of the water, the mass of the bromphenol blue, the mass of the oxalic acid and the volume ratio of the ethanol is preferably 250ml:0.5g:0.5g:0.8g:50ml. The raw materials, the method and the like of the cow dung compost are not particularly limited, and the cow dung compost can be routinely used by a person skilled in the art.
The invention provides a preparation method of the color developing agent, which comprises the following steps: and dissolving the bromocreosote blue, the bromophenol blue and the oxalic acid in ethanol, and then mixing with water to obtain the color developing agent.
The invention also provides application of the color developing agent in detecting compost maturity.
The invention also provides a kit for detecting the compost maturity, which comprises a detection device, a color card and the color reagent according to the technical scheme; the detection device comprises a container 1 and a container 2, and n breather holes are formed in the middle of the detection device. In the present invention, the detection device is shown in fig. 1, and the color card is shown in fig. 2.
The invention also provides a method for detecting the compost maturity, which comprises the following steps: placing a compost sample into a container 1 of the detection device of the kit according to the technical scheme, placing the color developing agent into a container 2, and comparing the color developing card after 15min to obtain the decomposition degree of the compost sample. In the present invention, the amount of the compost sample to be placed is preferably 4g. In the present invention, the amount of the developer to be placed is preferably 0.5ml.
The present invention will be described in detail with reference to examples for further illustration of the invention, but they should not be construed as limiting the scope of the invention.
Example 1
Chicken manure color development liquid preparation: 1g of bromocreosote blue, 1g of bromophenol blue, and 2g of oxalic acid were weighed out in 50mL of ethanol solution. Then, the color development solution was fixed to 300mL with distilled water and stored at 4℃in the absence of light.
Example 2
Preparing sheep manure color development liquid: 0.7g of bromocreosote blue, and 1.2g of oxalic acid were weighed out in 50mL of ethanol solution. Then distilled water is used for fixing the volume of the color development liquid to 300mL, and the color development liquid is preserved at 4 ℃ in dark place;
example 3
Preparing cow dung chromogenic liquid: 0.5g of bromocreosote blue, and 0.8g of oxalic acid were weighed out in 50mL of ethanol solution. Then, the color development solution was fixed to 300mL with distilled water and stored at 4℃in the absence of light.
Example 4
Composting and color development experiment of chicken manure:
and (3) performing color development detection on compost samples (chicken manure is used as a raw material, straw is used as an auxiliary material and conventional composting) of compost 0, 6, 10, 15, 25 and 50 d. Sampling was performed by the quarter method, and 4g of compost samples at different stages were weighed into vessel 1, while 0.5mL of color development solution was introduced into vessel 2. The color development was observed for 15 min. Compost samples of compost 0, 6, 10, 15, 25, 50d developed purple, purplish, pale purple, pale green, yellow, respectively. The conditions of the contrast color card are respectively non-decomposed, half-decomposed, medium-decomposed and complete-decomposed. Meanwhile, the germination indexes of seeds of the compost samples tested by using organic fertilizer NY/T525-2021 are 2.1%, 13.6%, 43.7%, 54.1%, 76.3% and 90.9% respectively. The detection method has the advantages that the detection of the maturity is consistent with the detection of industry standards, the detection method can be applied to the detection of the maturity of chicken manure, meanwhile, the detection time is shorter, and the detection method is simpler.
Example 5
Composting and color development experiment of sheep manure:
and (3) performing color development detection on compost samples (sheep manure is used as a raw material, straw is used as an auxiliary material and conventional composting) of compost 0, 7, 14, 21 and 30 d. Sampling was performed by the quarter method, and 4g of compost samples at different stages were weighed into vessel 1, while 0.5mL of color development solution was introduced into vessel 2. The color development was observed for 15 min. Compost samples of compost 0, 7, 14, 21, 30d developed purple, light green, respectively. The conditions of the contrast color card are respectively non-decomposed, half-decomposed, medium-decomposed and medium-decomposed. Meanwhile, the germination indexes of seeds of the compost samples tested by using organic fertilizer NY/T525-2021 are 5.2%, 20.3%, 64%, 79.2% and 81.6% respectively. The detection method has the advantages that the detection of the decomposition degree is consistent with the detection of industry standards, the detection method can be applied to the detection of the decomposition degree of sheep manure, meanwhile, the detection time is shorter, and the detection method is simpler.
Example 6
Composting and developing experiment of cow dung:
and (3) performing color development detection on compost samples (sheep manure is used as a raw material and straw is used as an auxiliary material) of compost 0, 5, 10, 15, 20 and 25 d. Samples were taken by the quarter method, 10g of compost samples from different stages were weighed into vessel 1, and 0.5mL of color development solution was added to vessel 2. The color development was observed for 30 min. Compost samples of compost 0, 5, 10, 15, 20, 25d developed purple, purplish, pale purple, pale green, yellow, respectively. The conditions of the contrast color card are respectively non-decomposed, half-decomposed, medium-decomposed and completely decomposed. Meanwhile, the germination indexes of seeds of the compost samples tested by using organic fertilizer NY/T525-2021 are 25.7%, 43.3%, 67.1%, 70.3%, 81.6% and 93.2% respectively. The detection method has the advantages that the detection of the maturity is consistent with the detection of industry standards, the detection method can be applied to the detection of the maturity of cow dung, meanwhile, the detection time is shorter, and the detection method is simpler.
Example 7
The prior art is taken as a comparative example to explain the scheme of the invention to solve the problems of the prior art
Preparation of CO 2 Gel test paper (CN 110412023A), NH 3 Gel test paper (CN 110412023A) is used for testing the color development results and corresponding decomposition conditions of the compost samples such as chicken manure, cow manure, sheep manure and the like in examples 4, 5 and 6 shown in Table 1:
TABLE 1 decomposition of different compost samples
Category(s) | NH 3 Test paper color | CO 2 Test paper color | Degree of maturity of | Index of germination of seeds |
Chicken manure for 0 day | Blue light | Yellow colour | Not decomposed | 2.1% |
Chicken manure for 6 days | Blue light | Yellow colour | Not decomposed | 13.6% |
Chicken manure for 10 days | Blue light | Yellow colour | Not decomposed | 43.7% |
Chicken manure for 15 days | Blue light | Yellow colour | Not decomposed | 54.1% |
Chicken manure for 25 days | Blue light | Yellow colour | Not decomposed | 76.3% |
Chicken manure for 50 days | Blue light | Yellow colour | Not decomposed | 90.9% |
Sheep manure for 0 day | Blue light | Yellow colour | Not decomposed | 5.2% |
Sheep manure for 7 days | Blue light | Yellow colour | Not decomposed | 20.3% |
Sheep manure for 14 days | Blue light | Yellow colour | Not decomposed | 64% |
Sheep manure for 21 days | Blue light | Yellow colour | Not decomposed | 79.2% |
Sheep manure for 30 days | Blue light | Yellow colour | Not decomposed | 81.6% |
Cow dung for 0 day | Blue light | Yellow colour | Not decomposed | 25.7% |
Cow dung for 5 days | Blue light | Yellow colour | Not decomposed | 43.3% |
Cow dung for 10 days | Blue light | Yellow colour | Not decomposed | 67.1% |
Cow dung for 15 days | Blue light | Red colour | Medium and low curing | 70.3% |
Cow dung for 20 days | Blue light | Red colour | Medium and low curing | 81.6% |
Cow dung for 25 days | Blue light | Red colour | Medium and low curing | 93.2% |
As can be seen from table 1, compared with the prior patent, the prior patent tests the decomposition degree of chicken manure, sheep manure and cow manure, and the test result of the prior patent is larger than the actual test result of the germination index of seeds.
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.
Claims (10)
1. The color reagent for detecting the compost maturity is characterized by comprising water, bromocreosote blue, bromophenol blue, oxalic acid and ethanol;
the volume ratio of water, the mass of the bromocreosote blue, the mass of oxalic acid and the volume ratio of ethanol is 250ml to 0.5-1 g to 0.8-2 g to 50ml.
2. The color developer according to claim 1, wherein when the raw material for composting is chicken manure, the volume ratio of water, mass of bromphenol blue, mass of oxalic acid and volume ratio of ethanol is 250ml:1g:1g:2g:50ml.
3. The developer according to claim 1, wherein when the compost raw material is sheep manure, the volume ratio of water, mass of bromphenol blue, mass of bromophenol blue, mass of oxalic acid and volume ratio of ethanol is 250ml:0.7g:0.7g:1.2g:50ml.
4. The developer according to claim 1, wherein when the raw material for composting is cow dung, the volume ratio of water, mass of bromphenol blue, mass of bromophenol blue, mass of oxalic acid and volume ratio of ethanol is 250ml:0.5g:0.5g:0.8g:50ml.
5. A process for producing a color developer according to any one of claims 1 to 4, comprising the steps of: and dissolving the bromocreosote blue, the bromophenol blue and the oxalic acid in ethanol, and then mixing with water to obtain the color developing agent.
6. Use of a color developer according to any one of claims 1 to 4 for detecting compost maturity.
7. A kit for detecting compost maturity, comprising a detection device, a color card and the color reagent of any one of claims 1-4;
the detection device comprises a container 1 and a container 2, and n breather holes are formed in the middle of the detection device.
8. A method for detecting compost maturity, comprising the steps of: placing a compost sample into a container 1 of the detection device of the kit of claim 7, placing the color developing agent into a container 2, and comparing the color developing card after 15min to obtain the maturity of the compost sample.
9. The method of claim 8, wherein the compost sample is placed in an amount of 4g.
10. The method of claim 8, wherein the developer is placed in an amount of 0.5ml.
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