CN116850080A - Gel mask containing recombinant humanized collagen and preparation method thereof - Google Patents
Gel mask containing recombinant humanized collagen and preparation method thereof Download PDFInfo
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- CN116850080A CN116850080A CN202310888210.8A CN202310888210A CN116850080A CN 116850080 A CN116850080 A CN 116850080A CN 202310888210 A CN202310888210 A CN 202310888210A CN 116850080 A CN116850080 A CN 116850080A
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- recombinant humanized
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- humanized collagen
- skin
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- 108010035532 Collagen Proteins 0.000 title claims abstract description 65
- 102000008186 Collagen Human genes 0.000 title claims abstract description 64
- 229920001436 collagen Polymers 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000004530 micro-emulsion Substances 0.000 claims abstract description 46
- 239000002131 composite material Substances 0.000 claims abstract description 45
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 claims abstract description 44
- 239000010630 cinnamon oil Substances 0.000 claims abstract description 30
- 239000004006 olive oil Substances 0.000 claims abstract description 28
- 235000008390 olive oil Nutrition 0.000 claims abstract description 28
- IELOKBJPULMYRW-NJQVLOCASA-N D-alpha-Tocopheryl Acid Succinate Chemical compound OC(=O)CCC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C IELOKBJPULMYRW-NJQVLOCASA-N 0.000 claims abstract description 26
- 229940099418 d- alpha-tocopherol succinate Drugs 0.000 claims abstract description 26
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 24
- 239000004310 lactic acid Substances 0.000 claims abstract description 24
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 claims abstract description 22
- 229960000458 allantoin Drugs 0.000 claims abstract description 22
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 22
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 17
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 17
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 17
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 17
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 229940074410 trehalose Drugs 0.000 claims abstract description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 42
- 238000002156 mixing Methods 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 7
- 229920002125 Sokalan® Polymers 0.000 claims description 7
- 229960001631 carbomer Drugs 0.000 claims description 7
- 229940049638 carbomer homopolymer type c Drugs 0.000 claims description 7
- 229940043234 carbomer-940 Drugs 0.000 claims description 7
- 229960005150 glycerol Drugs 0.000 claims description 7
- 238000005303 weighing Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 7
- 230000037303 wrinkles Effects 0.000 abstract description 6
- 238000013329 compounding Methods 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 230000002195 synergetic effect Effects 0.000 abstract description 4
- 201000004624 Dermatitis Diseases 0.000 abstract description 3
- 206010061218 Inflammation Diseases 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 3
- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 230000004054 inflammatory process Effects 0.000 abstract description 3
- 230000037314 wound repair Effects 0.000 abstract description 3
- 230000003467 diminishing effect Effects 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 239000000499 gel Substances 0.000 description 35
- 230000000052 comparative effect Effects 0.000 description 19
- 210000003491 skin Anatomy 0.000 description 19
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 15
- 238000012360 testing method Methods 0.000 description 11
- 230000001186 cumulative effect Effects 0.000 description 6
- 239000012467 final product Substances 0.000 description 5
- 238000003760 magnetic stirring Methods 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 102000001187 Collagen Type III Human genes 0.000 description 2
- 108010069502 Collagen Type III Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000013558 reference substance Substances 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 102000000503 Collagen Type II Human genes 0.000 description 1
- 108010041390 Collagen Type II Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010040830 Skin discomfort Diseases 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
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- 239000002390 adhesive tape Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
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- 210000004027 cell Anatomy 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
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- 210000004207 dermis Anatomy 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 150000002632 lipids Chemical group 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 230000036560 skin regeneration Effects 0.000 description 1
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- 239000004094 surface-active agent Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
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- 230000005951 type IV hypersensitivity Effects 0.000 description 1
- 208000027930 type IV hypersensitivity disease Diseases 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
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- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- Pain & Pain Management (AREA)
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Abstract
The invention relates to a gel mask containing recombinant humanized collagen and a preparation method thereof, belonging to the technical field of gel masks. The raw materials of the invention comprise recombinant humanized collagen, composite microemulsion, trehalose, sodium hyaluronate, allantoin, beta-glucose and lactic acid, are especially suitable for wound repair, can also be used for sensitive diseases on the surface of skin, help diminishing inflammation and sterilizing, promote the regression of skin inflammation, and have the effects of moistening and resisting wrinkles; the composite microemulsion is prepared by compounding three substances, namely olive oil, cinnamon oil and vitamin E succinate, so that the acting time of the recombinant humanized collagen is prolonged, and the skin retention of the recombinant humanized collagen is improved; the cinnamon oil and the lactic acid are synergistic, so that the cinnamon oil and the lactic acid have good antibacterial and anti-inflammatory effects, have the effects of tightening skin and reducing fine wrinkles, and promote skin self-repairing and recovering under the synergistic assistance of allantoin, beta-glucose and olive oil.
Description
Technical Field
The invention belongs to the technical field of gel masks, and particularly relates to a gel mask containing recombinant humanized collagen and a preparation method thereof.
Background
Collagen is an important component in life activities, has an important supporting effect on maintaining the structure and function of skin tissues, and mainly comprises type I and type III collagen in skin, wherein type III collagen is a new-born collagen, and can promote skin regeneration. The recombinant human III type collagen (Recombinant humancollagen III, rhc-III) is a protein which is obtained by using a gene engineering technology, taking a human III type collagen gene sequence as a template, carrying out optimized modification and recombinant expression, has the same parts as the corresponding parts of the natural III type collagen amino acid sequence, has high bioactivity and biocompatibility, and has good water solubility. Studies show that Rhc-III can promote full-layer wound healing and angiogenesis, promote ordered arrangement of collagen fibers of new dermis tissues, and has the functions of repairing skin wounds and improving wound healing quality.
However, the water-soluble recombinant humanized collagen is difficult to penetrate the lipid structure among the cells of the horny layer, has short action time, poor skin retention and inconvenient use, and has the defect of limiting the use of the water-soluble recombinant humanized collagen in skin care products.
Disclosure of Invention
The invention relates to a gel mask containing recombinant humanized collagen and a preparation method thereof, belonging to the technical field of gel masks. The raw materials of the invention comprise recombinant humanized collagen, composite microemulsion, trehalose, sodium hyaluronate, allantoin, beta-glucose and lactic acid, are especially suitable for wound repair, can also be used for sensitive diseases on the surface of skin, help diminishing inflammation and sterilizing, promote the regression of skin inflammation, and have the effects of moistening and resisting wrinkles; the composite microemulsion is prepared by compounding three substances, namely olive oil, cinnamon oil and vitamin E succinate, so that the acting time of the recombinant humanized collagen is prolonged, and the skin retention of the recombinant humanized collagen is improved; the cinnamon oil and the lactic acid are synergistic, so that the cinnamon oil and the lactic acid have good antibacterial and anti-inflammatory effects, have the effects of tightening skin and reducing fine wrinkles, and promote skin self-repairing and recovering under the synergistic assistance of allantoin, beta-glucose and olive oil.
The aim of the invention can be achieved by the following technical scheme:
a gel mask containing recombinant humanized collagen comprises the following raw materials in parts by weight:
5-8 parts of recombinant humanized collagen, 5-8 parts of composite microemulsion, 8-12 parts of glycerol, 1-2 parts of lactic acid, 3-5 parts of trehalose, 0.1-0.5 part of sodium hyaluronate, 0.5-1.5 parts of allantoin, 1-2 parts of beta-glucose and 1-9400.8 parts of carbomer.
As a preferable scheme of the invention, the composite microemulsion comprises olive oil, vitamin E succinate and cinnamon oil, wherein the mass ratio of the olive oil to the vitamin E succinate to the cinnamon oil is 2-4:5-6:0.2-0.5.
As a preferable scheme of the invention, the preparation method of the composite microemulsion comprises the following operations: weighing olive oil, cinnamon oil and vitamin E succinate, mixing, heating, and adding deionized water to obtain the composite microemulsion.
As a preferred embodiment of the present invention, the heating temperature is 45-60 ℃.
As a preferable scheme of the invention, the deionized water is added dropwise, and stirring is carried out while adding until the deionized water is clear and transparent.
The preparation method of the gel mask comprises the following operations:
mixing recombinant humanized collagen, composite microemulsion, glycerol, trehalose, sodium hyaluronate, allantoin, beta-glucose and lactic acid, stirring to obtain a mixture, adding the mixture into carbomer-940, mixing uniformly, adjusting pH, centrifuging, and removing bubbles.
As a preferred embodiment of the present invention, the pH is adjusted to 5.3-5.6.
As a preferred embodiment of the present invention, the centrifugation is performed as follows: at 3000 r.min -1 Centrifuging for 10-12min.
The invention has the beneficial effects that:
1. the invention provides a gel mask containing recombinant humanized collagen, which comprises raw materials of the recombinant humanized collagen, composite microemulsion, trehalose, sodium hyaluronate, allantoin, beta-glucose and lactic acid, is particularly suitable for wound repair, can relieve local pain symptoms of the face, can recover the skin as soon as possible, can also be used for sensitive diseases on the skin surface, helps diminish inflammation and sterilize, and promotes the resolution of skin inflammation, and meanwhile, the gel mask is rich in unsaturated fatty acid and multiple vitamins, and has the effects of moistening and resisting wrinkles.
2. In the invention, the composite microemulsion is prepared by compounding three substances of olive oil, cinnamon oil and vitamin E succinate, and the three substances are mutually solubilized, so that the application of alcohol in a surfactant is avoided, and the safety and wide applicability of the obtained gel mask are enhanced.
3. The compound microemulsion is prepared by compounding the olive oil, the cinnamon oil and the vitamin E succinate, can effectively carry the recombinant humanized collagen deep into the skin, increases the acting time of the recombinant humanized collagen, and improves the skin retention of the recombinant humanized collagen.
4. According to the invention, cinnamon oil and lactic acid are cooperated, so that the cinnamon oil and lactic acid have good antibacterial and anti-inflammatory effects, have the effects of tightening skin and weakening fine wrinkles, promote skin self-repairing and recovering under the cooperation assistance of allantoin, beta-glucose and olive oil, promote regeneration of epidermal cells, help to relieve skin discomfort, and are mild and not stimulated to skin.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
A gel mask containing recombinant humanized collagen comprises the following raw materials in parts by weight:
5 parts of recombinant humanized collagen, 5 parts of composite microemulsion, 8 parts of glycerol, 1 part of lactic acid, 3 parts of trehalose, 0.1 part of sodium hyaluronate, 0.5 part of allantoin, 1 part of beta-glucose and 9400.8 parts of carbomer.
Wherein the composite microemulsion comprises olive oil, vitamin E succinate and cinnamon oil, and the mass ratio of the olive oil to the vitamin E succinate to the cinnamon oil is 2:5:0.2.
The preparation method of the composite microemulsion comprises the following operations: weighing olive oil, cinnamon oil and vitamin E succinate, vortex mixing, heating to 45 ℃, and then dropwise adding deionized water under magnetic stirring until the mixture is clear and transparent, thus obtaining the composite microemulsion.
The preparation method of the gel mask comprises the following operations:
mixing recombinant humanized collagen, composite microemulsion, glycerol, trehalose, sodium hyaluronate, allantoin, beta-glucose, and lactic acid, stirring to obtain a mixture, adding the mixture into carbomer-940, mixing, adjusting pH to 5.3-5.6 with triethylamine, and adjusting pH to 3000 r.min -1 Centrifuging for 10min to obtain the final product.
Example 2
A gel mask containing recombinant humanized collagen comprises the following raw materials in parts by weight:
6 parts of recombinant humanized collagen, 6 parts of composite microemulsion, 9 parts of glycerol, 1.2 parts of lactic acid, 3.5 parts of trehalose, 0.2 part of sodium hyaluronate, 0.8 part of allantoin, 1.2 parts of beta-glucose and 9400.85 parts of carbomer.
Wherein the composite microemulsion comprises olive oil, vitamin E succinate and cinnamon oil, and the mass ratio of the olive oil to the vitamin E succinate to the cinnamon oil is 2.5:5.2:0.25.
The preparation method of the composite microemulsion comprises the following operations: and weighing olive oil, cinnamon oil and vitamin E succinate, vortex mixing, heating to 48 ℃, and then dropwise adding deionized water under magnetic stirring until the mixture is clear and transparent, thus obtaining the composite microemulsion.
The preparation method of the gel mask comprises the following operations:
mixing recombinant humanized collagen, composite microemulsion, glycerol, trehalose, sodium hyaluronate, allantoin, beta-glucose, and lactic acid, stirring to obtain a mixture, adding the mixture into carbomer-940, mixing, adjusting pH to 5.3-5.6 with triethylamine, and adjusting pH to 3000 r.min -1 Centrifuging for 10min to obtain the final product.
Example 3
A gel mask containing recombinant humanized collagen comprises the following raw materials in parts by weight:
6.5 parts of recombinant humanized collagen, 6.5 parts of composite microemulsion, 10 parts of glycerol, 1.5 parts of lactic acid, 4 parts of trehalose, 0.3 part of sodium hyaluronate, 1 part of allantoin, 1.5 parts of beta-glucose and 9400.9 parts of carbomer.
Wherein the composite microemulsion comprises olive oil, vitamin E succinate and cinnamon oil, and the mass ratio of the olive oil to the vitamin E succinate to the cinnamon oil is 2-4:5-6:0.2-0.5.
The preparation method of the composite microemulsion comprises the following operations: and weighing olive oil, cinnamon oil and vitamin E succinate, vortex mixing, heating to 52 ℃, and then dropwise adding deionized water under magnetic stirring until the mixture is clear and transparent, thus obtaining the composite microemulsion.
The preparation method of the gel mask comprises the following operations:
mixing recombinant humanized collagen, composite microemulsion, glycerol, trehalose, sodium hyaluronate, allantoin, beta-glucose, and lactic acid, stirring to obtain a mixture, adding the mixture into carbomer-940, mixing, adjusting pH to 5.3-5.6 with triethylamine, and adjusting pH to 3000 r.min -1 Centrifuging for 11min to obtain the final product.
Example 4
A gel mask containing recombinant humanized collagen comprises the following raw materials in parts by weight:
7 parts of recombinant humanized collagen, 7 parts of composite microemulsion, 11 parts of glycerol, 1.8 parts of lactic acid, 4.5 parts of trehalose, 0.4 part of sodium hyaluronate, 1.2 parts of allantoin, 1.8 parts of beta-glucose and 9400.95 parts of carbomer.
Wherein the composite microemulsion comprises olive oil, vitamin E succinate and cinnamon oil, and the mass ratio of the olive oil to the vitamin E succinate to the cinnamon oil is 3.5:5.8:0.4.
The preparation method of the composite microemulsion comprises the following operations: and weighing olive oil, cinnamon oil and vitamin E succinate, vortex mixing, heating to 58 ℃, and then dropwise adding deionized water under magnetic stirring until the mixture is clear and transparent to prepare the composite microemulsion.
The preparation method of the gel mask comprises the following operations:
mixing recombinant humanized collagen, composite microemulsion, glycerol, trehalose, sodium hyaluronate, allantoin, beta-glucose, and lactic acid, stirring to obtain a mixture, adding the mixture into carbomer-940, mixing, adjusting pH to 5.3-5.6 with triethylamine, and adjusting pH to 3000 r.min -1 Centrifuging for 12min to obtain the final product.
Example 5
A gel mask containing recombinant humanized collagen comprises the following raw materials in parts by weight:
8 parts of recombinant humanized collagen, 8 parts of composite microemulsion, 12 parts of glycerol, 2 parts of lactic acid, 5 parts of trehalose, 0.5 part of sodium hyaluronate, 1.5 parts of allantoin, 2 parts of beta-glucose and 9401 parts of carbomer.
Wherein the composite microemulsion comprises olive oil, vitamin E succinate and cinnamon oil, and the mass ratio of the olive oil to the vitamin E succinate to the cinnamon oil is 4:6:0.5.
The preparation method of the composite microemulsion comprises the following operations: and weighing olive oil, cinnamon oil and vitamin E succinate, heating to 60 ℃ after vortex mixing, and then dropwise adding deionized water under magnetic stirring until the mixture is clear and transparent, thus obtaining the composite microemulsion.
The preparation method of the gel mask comprises the following operations:
mixing recombinant humanized collagen, composite microemulsion, glycerol, trehalose, sodium hyaluronate, allantoin, beta-glucose, and lactic acid, stirring to obtain a mixture, adding the mixture into carbomer-940, mixing, adjusting pH to 5.3-5.6 with triethylamine, and adjusting pH to 3000 r.min -1 Centrifuging for 12min to obtain the final product.
Comparative example 1
A gel mask containing recombinant humanized collagen was prepared in the same manner as in example 5 except that the gel mask contained no composite microemulsion as in example 5.
Comparative example 2
A gel mask containing recombinant humanized collagen is prepared by substituting oleum Cinnamomi for oleum Olivarum in the composite microemulsion compared with example 5, and the rest is the same as in example 5.
Comparative example 3
A gel mask containing recombinant humanized collagen is prepared by substituting vitamin E succinate for oleum Cinnamomi in the composite microemulsion compared with example 5, and the rest is the same as example 5.
Comparative example 4
A gel mask containing recombinant humanized collagen was prepared by substituting olive oil for vitamin E succinate in the composite microemulsion as in example 5, and the rest was the same as in example 5.
Comparative example 5
A gel mask containing recombinant humanized collagen was prepared as in example 5, except that lactic acid was not contained.
Comparative example 6
A gel mask containing recombinant humanized collagen was prepared by substituting beta-glucose for allantoin in comparison with example 5, and the rest was the same as in example 5.
Comparative example 7
A gel mask containing recombinant humanized collagen was prepared by substituting allantoin for beta-glucose as in example 5, and the rest was the same as in example 5.
The following tests were performed on examples 1-5 and comparative examples 1-7:
test example 1 evaluation of moisturizing Effect
The medical breathable adhesive tape simulates the skin of a real person, 0.2g of sample is weighed and uniformly smeared on the skin, a certain moisturizing gel mask sold in the market is taken as a reference substance, the reference substance is placed into a color-changing silica gel dryer with the relative humidity (RelativeHumidity, RH) of 50%, the color-changing silica gel dryer is placed for 8 hours, data are weighed and recorded, and the moisturizing rate at the moment is calculated. The data were analyzed using SPSSStatistics24.0, and the results obtained by comparing the data with each other using t-test and calculating the moisture retention after measurement are shown in Table 1.
Wherein: m is m T，RH At RH%, the mass of water after 8 hours of standing;
m 0，RH at RH%, the mass of water after 0 hours of standing.
TABLE 1
| Test group | Moisture retention/% |
| Example 1 | 78.2 |
| Example 2 | 78.6 |
| Example 3 | 78.4 |
| Example 4 | 79.0 |
| Example 5 | 79.4 |
| Comparative example 1 | 70.4 |
| Comparative example 2 | 73.6 |
| Comparative example 3 | 75.1 |
| Comparative example 4 | 73.2 |
| Comparative example 5 | 77.9 |
| Comparative example 6 | 78.1 |
| Comparative example 7 | 77.6 |
As can be seen from Table 1, the gel mask containing the recombinant humanized collagen provided by the invention has good moisture retention rate in examples 1 to 5, the gel mask containing the recombinant humanized collagen provided by comparative examples 1 to 4 has different degrees of reduction in moisture retention rate, and the gel mask containing the recombinant humanized collagen provided by comparative examples 5 to 7 has little change in moisture retention rate.
Test example 2 skin irritation test
The test is carried out according to the test method of stimulus and delayed type hypersensitivity test of GB/T16886.10-2017: under the test conditions, no red swelling reaction was found to be caused by the test sample; the primary stimulation index was 0.
Test example 3 penetration test
Instrument: and performing an in-vitro transdermal experiment by adopting a TPY-2 intelligent transdermal diffusion instrument.
Experimental materials: intact and damaged rat isolated skin;
and (3) receiving liquid: PBS pH7.4
Temperature: (32+ -0.5) deg.C;
rotational speed: 300rmp
Experimental method the gel masks prepared in examples 1-5 and comparative examples 1-7 were applied to the isolated skin uniformly and the foam was removed, and 0.5mL of the receiving solution was taken out at the preset time points (2, 4, 8, 12 and 24 h) to be tested, and the same volume of the receiving solution was replenished. The absorbance of the extracted samples was measured by the BCA method, and the absorbance of each group was subtracted from the background absorbance, and then substituted into a standard curve equation, and the concentration of total collagen was calculated. The cumulative transdermal penetration per unit area (Qn, μg/cm) was calculated for 24 hours as follows 2 ) The cumulative transdermal percentage (Qr,%) for 24h was calculated as follows, and the results obtained are shown in table 2.
In the above, Q n Accumulating the transdermal flux per unit area, C n For the concentration of collagen at the nth sampling, V is the receiving pool volume (6 mL), C i For the collagen concentration at the ith sampling, V i For the sampling volume, A is the effective contact area (2.5 cm 2 )，Q r To accumulatePercent (%) transdermal, W is the total amount of collagen.
TABLE 2
As can be seen from Table 2, the gel mask containing recombinant humanized collagen provided by the present invention has good cumulative transdermal amount and cumulative transdermal percentage per unit area in examples 1 to 5, and the gel mask containing recombinant humanized collagen provided by comparative examples 1 to 7 has various degrees of decrease in cumulative transdermal amount and cumulative transdermal percentage per unit area.
In the description of the present specification, the descriptions of the terms "one embodiment," "example," "specific example," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is merely illustrative and explanatory of the invention, as various modifications and additions may be made to the particular embodiments described, or in a similar manner, by those skilled in the art, without departing from the scope of the invention or exceeding the scope of the invention as defined in the claims.
Claims (8)
1. The gel mask containing the recombinant humanized collagen is characterized by comprising the following raw materials in parts by weight:
5-8 parts of recombinant humanized collagen, 5-8 parts of composite microemulsion, 8-12 parts of glycerol, 1-2 parts of lactic acid, 3-5 parts of trehalose, 0.1-0.5 part of sodium hyaluronate, 0.5-1.5 parts of allantoin, 1-2 parts of beta-glucose and 1-9400.8 parts of carbomer.
2. The gel mask containing the recombinant humanized collagen according to claim 1, wherein the composite microemulsion comprises olive oil, vitamin E succinate and cinnamon oil in a mass ratio of 2-4:5-6:0.2-0.5.
3. The gel mask containing recombinant humanized collagen according to claim 2, wherein the preparation method of the composite microemulsion comprises the following operations: weighing olive oil, cinnamon oil and vitamin E succinate, mixing, heating, and adding deionized water to obtain the composite microemulsion.
4. A gel pack containing recombinant humanized collagen according to claim 3, wherein the heating temperature is 45-60 ℃.
5. A gel pack containing recombinant humanized collagen according to claim 3, wherein deionized water is added dropwise with stirring until clear and transparent.
6. A method of preparing a gel mask according to any one of claims 1 to 5, comprising the steps of:
mixing recombinant humanized collagen, composite microemulsion, glycerol, trehalose, sodium hyaluronate, allantoin, beta-glucose and lactic acid, stirring to obtain a mixture, adding the mixture into carbomer-940, mixing uniformly, adjusting pH, centrifuging, and removing bubbles.
7. The method of claim 6, wherein the pH is adjusted to 5.3-5.6.
8. The method for preparing a gel mask according to claim 6, wherein the centrifugation is performed as follows: at 3000 r.min -1 Centrifuging for 10-12min.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003128521A (en) * | 2001-10-16 | 2003-05-08 | Rohto Pharmaceut Co Ltd | External preparation |
| CN102448450A (en) * | 2009-04-09 | 2012-05-09 | 西姆莱斯有限公司 | Compositions comprising trans-tert-butyl cyclohexanol as skin irritation-reducing agent |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003128521A (en) * | 2001-10-16 | 2003-05-08 | Rohto Pharmaceut Co Ltd | External preparation |
| CN102448450A (en) * | 2009-04-09 | 2012-05-09 | 西姆莱斯有限公司 | Compositions comprising trans-tert-butyl cyclohexanol as skin irritation-reducing agent |
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