CN116840194A - Micro analyzer based on self-compensating near infrared SPR effect - Google Patents

Abstract

The invention discloses a micro analyzer based on self-compensating near-infrared SPR effect, and relates to the field of SPR sensing technology. The micro analyzer includes a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module; the laser emission module emits C-band laser; light intensity self-compensation The module divides the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect; the multi-channel detection module simultaneously detects a variety of samples to be tested; the SPR excitation module generates multi-channel reflections with the SPR effect Light; the multi-channel photoelectric detection module detects the light intensity signal of s-polarized light and multi-channel reflected light; the signal processing module determines the refractive index and concentration of the sample to be measured based on the light intensity signal; the result display module displays the refractive index and concentration. The invention can reduce the cost and volume of the instrument while achieving high sensitivity, high precision and rapid detection.

Description

A micro-analyzer based on self-compensating near-infrared SPR effect

Technical field

The invention relates to the field of SPR sensing technology, and in particular to a micro-analyzer based on self-compensating near-infrared SPR effect.

Background technique

SPR (Surface Plasmon Resonance) technology is an important bioanalytical technology that can detect interactions between biomolecules in real time. SPR technology is based on the adsorption of biomolecules on metal surfaces. It uses changes in reflected light intensity caused by surface plasmon waves generated by laser irradiation to detect interactions between biomolecules. It is widely used in fields such as drug screening, biosensing, and biomedical research. .

Currently, high-performance SPR analyzers on the market are generally larger and more expensive, which limits the popularity of SPR analyzers.

Contents of the invention

The purpose of the present invention is to provide a micro-analyzer based on the self-compensating near-infrared SPR effect to achieve high sensitivity, high precision and rapid detection while reducing the cost and volume of the instrument.

In order to achieve the above objects, the present invention provides the following solutions:

A micro-analyzer based on self-compensating near-infrared SPR effect, including: laser emission module, light intensity self-compensation module, multi-channel detection module, SPR excitation module, multi-channel photoelectric detection module, signal processing module and result display module;

The laser emission module is used to emit C-band laser, and incident the C-band laser into the light intensity self-compensation module;

The light intensity self-compensation module is used to divide the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect, and to incident the p-polarized light into the SPR excitation module, Inject the s-polarized light into the multi-channel photoelectric detection module;

The multi-channel detection module includes multiple sample test channels and non-specific reference channels, which are used to detect multiple samples to be tested at the same time;

The SPR excitation module uses p-polarized light to excite the SPR effect of the sample to be measured and generates multi-channel reflected light with the SPR effect, and injects the multi-channel reflected light into the multi-channel photoelectric detection module;

The multi-channel photoelectric detection module is used to detect the light intensity signals of the s-polarized light and the multi-channel reflected light and send them to the signal processing module;

The signal processing module is used to determine the refractive index and concentration of the sample to be measured based on the light intensity signal and send it to the result display module;

The result display module is used to display the refractive index and concentration.

Optionally, the laser emission module includes: a fiber laser transmitter, a transmission fiber, a C-band laser diode, an incident light fiber collimator, and a collimator bracket;

The fiber laser transmitter is connected to the C-band laser diode through the transmission fiber; the fiber laser transmitter is used to generate laser light and incident on the C-band laser diode through the transmission fiber. Diodes produce C-band laser light;

The incident light fiber collimator is installed on the collimator bracket; the collimator bracket is used to fix the incident light fiber collimator to the C-band laser incident to the light intensity self-compensation On the optical path of the module, the incident light fiber collimating mirror is used to collimate the C-band laser and expand the beam diameter.

Optionally, the light intensity self-compensation module includes: a polarizing beam splitting prism and a polarizing beam splitting prism bracket;

The polarizing beam splitting prism is installed in the polarizing beam splitting prism bracket; the polarizing beam splitting prism bracket is cooperatively connected with the collimating mirror bracket through threads;

The polarization beam splitter divides the C-band laser into s-polarized light that cannot stimulate the SPR effect and p-polarized light that can stimulate the SPR effect.

Optionally, the SPR excitation module includes: a semi-cylindrical prism bracket, a semi-cylindrical prism and a sensing chip; the sensing chip includes a sensing film and a glass sheet;

The semi-cylindrical prism is installed in the semi-cylindrical prism bracket; the semi-cylindrical prism bracket is cooperatively connected with the polarizing beam splitting prism bracket;

The glass sheet is located on the plane of the semi-cylindrical prism, and the semi-cylindrical prism and the glass sheet are coupled through a refractive index matching liquid; the sensing film is disposed on the glass sheet.

Optionally, the p-polarized light is incident obliquely on the sensor chip at a fixed angle, and the fixed angle is 62.77°-62.8°.

Optionally, the multi-channel detection module includes: a cover plate, a hose and a hose plug provided on the housing;

The cover plate is located on the top of the housing, and is provided with multiple partitions; the hose plug is provided with threads, and the hose is fixed to each partition of the cover plate through the hose plug. ;

The sensor chip is located directly below the cover plate, and each partition of the cover plate corresponds to the multiple channels on the sensor chip, including multiple sample test channels and non-specific reference channels; each sample test channel passes Different specific detection molecules are introduced into the corresponding hose to modify the sensing chip; the non-specific reference channel corresponds to the sensing membrane that is not modified with specific detection molecules.

Optionally, the multi-channel photoelectric detection module includes: a light intensity reference channel detector, a plurality of sample test channel detectors and a non-specific reference channel detector;

The light intensity reference channel detector is installed in the polarization beam splitter prism bracket and is located on the outgoing optical path of s-polarized light, used to detect the light intensity signal of s-polarized light;

The plurality of sample test channel detectors and non-specific reference channel detectors are installed on the semi-cylindrical prism bracket and are located on the outgoing optical path of the multi-channel reflected light.

Optionally, the signal processing module includes: a multi-channel signal converter, a microcontroller expansion board and a microcontroller connected in sequence;

The multi-channel signal converter is connected to the multi-channel photoelectric detection module and is used to convert multiple light intensity signals into multiple electrical signals; the multi-channel electrical signals are transmitted to the single chip microcomputer via the single chip expansion board;

The microcontroller is used to calculate the refractive index and concentration of various samples to be tested based on multiple electrical signals.

Optionally, the result display module includes: a display screen;

The display screen is located on the top of the housing and connected to the microcontroller, and is used to display the refractive index and concentration of various samples to be measured.

Optionally, the micro analyzer also includes: a power supply device, a charging port and a power switch;

The power supply device is located inside the housing; the charging port and the power switch are located on one side of the housing; the power supply device is connected to an external power supply through the charging port for charging; the power supply device is charged through the power switch They are respectively connected to the laser emission module, multi-channel photoelectric detection module, signal processing module and result display module for power supply.

According to the specific embodiments provided by the present invention, the present invention discloses the following technical effects:

The invention provides a micro analyzer based on the self-compensating near-infrared SPR effect, including a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display. module. Among them, the laser emission module is used to emit C-band laser and incident it to the light intensity self-compensation module; the light intensity self-compensation module is used to divide the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect. , and incident p-polarized light into the SPR excitation module, and incident s-polarized light into the multi-channel photoelectric detection module; the multi-channel detection module includes multiple sample test channels and non-specific reference channels, used to detect multiple samples to be tested at the same time; SPR The excitation module uses p-polarized light to excite the SPR effect of the sample to be tested and generates multi-channel reflected light with SPR effect, and injects the multi-channel reflected light into the multi-channel photoelectric detection module; the multi-channel photoelectric detection module is used to detect s-polarization The light intensity signal of light and multi-channel reflected light is sent to the signal processing module; the signal processing module is used to determine the refractive index and concentration of the sample to be measured based on the light intensity signal and sends it to the result display module; the result display module is used to analyze all The refractive index and concentration are displayed. The invention provides a micro-analyzer based on the self-compensating near-infrared SPR effect. Based on the near-infrared SPR effect, it realizes the detection of the concentration of the sample to be measured. Compared with the existing detection device using a spectrometer, it ensures the sensing performance. On the basis of reducing the size of the device; in addition, the present invention provides multiple test channels, which can detect multiple objects to be tested at the same time; and the self-compensation module and reference channel provided by the present invention greatly improve the sensitivity and stability of detection.

Description of the drawings

In order to explain the embodiments of the present invention or the technical solutions in the prior art more clearly, the drawings needed to be used in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some of the drawings of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained based on these drawings without exerting creative efforts.

Figure 1 is a schematic diagram of the overall structure of the micro analyzer provided by the present invention;

Figure 2 is a schematic diagram of the front and back of the sensing detection area of the micro analyzer provided by the present invention and an enlarged schematic diagram of the sensing film area;

Figure 3 is a schematic diagram of the external structure of the micro analyzer provided by the present invention;

Figure 4 is a schematic diagram of the housing dimensions of the micro analyzer provided by the present invention.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

Since high-performance SPR analyzers currently on the market are generally larger in size and more expensive, the popularity of SPR analyzers is limited to a certain extent. Therefore, it is of great significance to research and develop a SPR analyzer that combines miniaturization and high sensitivity, which can effectively reduce the cost and volume of the instrument while achieving high sensitivity, high precision and rapid detection. The purpose of the present invention is to provide a micro-analyzer based on the self-compensating near-infrared SPR effect to achieve high sensitivity, high precision and rapid detection while reducing the cost and volume of the instrument. The microanalyzer of the present invention can be used for rapid and accurate detection of biomolecules, and has the advantages of low cost, miniaturization, high precision, and ease of use.

In order to make the above objects, features and advantages of the present invention more obvious and understandable, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

Figure 1 is a schematic diagram of the overall structure of a micro analyzer based on the self-compensating near-infrared SPR effect provided by the present invention. Figure 2 is a schematic diagram of the front and back of the sensing detection area and an enlargement of the sensing film area of the micro analyzer provided by the present invention. Schematic diagram, as shown in Figures 1 and 2, the invention provides a micro analyzer based on self-compensating near-infrared SPR effect, including: laser emission module, light intensity self-compensation module, multi-channel detection module, SPR excitation module, Multi-channel photoelectric detection module, signal processing module and result display module.

Wherein, the laser emission module is used to emit C-band laser, and the C-band laser is incident on the light intensity self-compensation module.

Specifically, the laser emission module includes: a fiber laser transmitter 1, a transmission fiber 22, a C-band laser diode 2, an incident light fiber collimator 3 and a collimator bracket 19.

As shown in Figures 1 and 2, the fiber laser transmitter 1 is connected to the C-band laser diode 2 through the transmission fiber 22. Among them, the fiber laser transmitter 1 supplies power to the C-band laser diode 2 to generate laser light and adjust the laser intensity. The generated laser light is incident on the C-band laser diode 2 through the transmission fiber 22, and the C-band laser diode 2 generates C-band laser light. .

The incident light fiber collimating mirror 3 is installed on the collimating mirror bracket 19; the incident light fiber collimating mirror 3 is connected to the collimating mirror bracket 19 through threads, and the collimating mirror bracket 19 is used to fix the incident light fiber collimating mirror 3 On the optical path where the C-band laser is incident on the light intensity self-compensation module, it is used to collimate the C-band laser and expand the beam diameter so that the C-band laser can cover the entire sensor chip.

The light intensity self-compensation module is used to divide the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect, and to incident the p-polarized light into the SPR excitation module, The s-polarized light is incident on the multi-channel photoelectric detection module.

Specifically, the light intensity self-compensation module includes: a polarization beam splitter prism 4 and a polarization beam splitter prism bracket 18 . Among them, the polarizing beam splitting prism 4 is installed in the polarizing beam splitting prism bracket 18; the polarizing beam splitting prism bracket 18 is connected with the collimating mirror bracket 19 through threads.

The polarization beam splitter prism 4 divides the C-band laser into s-polarized light that cannot excite the SPR effect and p-polarized light that can excite the SPR effect. Among them, s-polarized light provides a reference signal for the detection signal to correct errors caused by light source fluctuations, etc. By referring to the light intensity, fluctuation and other signals of s-polarized light, the p-polarized light that stimulates the SPR effect is processed accordingly, which can reduce the errors in molecular detection signals caused by problems such as light source noise, chip preparation process, and non-specific adsorption.

Specifically, the multi-channel detection module includes multiple sample test channels and non-specific reference channels for detecting multiple samples to be tested simultaneously. The sensing channels of the present invention are divided into two types: one is a multi-channel sample test channel, and the other is a non-specific reference channel. In order to detect multiple samples to be tested at the same time, multiple sample testing channels need to be arranged. Before use, each sample test channel is fed with different specific detection molecules to modify the sensor chip; the non-specific reference channel corresponds to a sensor chip that is not modified with a metal sensing film with a specific detection molecule film. Except for the specific detection molecular film, the sensing chips of the multi-channel sample test channel and the non-specific reference channel have the same materials, gold film thickness and other parameters.

Referring to FIGS. 1 and 3 , the multi-channel detection module includes: a cover plate 17 provided on the housing 13 , a hose 24 and a hose plug 25 . Among them, the cover plate 17 is located on the top of the housing 13, and a plurality of partitions are provided on the cover plate 17 (three partitions are shown in Figure 3). The partitions on the cover plate 17 divide the sensing film 7 into multiple sample test channels and non-specific reference channels, which are used to simultaneously detect multiple samples to be tested and provide reference signals for detection signals to correct the results.

The hose plug 25 is provided with threads, and the hose 24 is fixed on each partition of the cover plate 17 through the hose plug 25 . The sensor chip is located directly below the cover plate 17. Each partition of the cover plate 17 corresponds to multiple channels on the sensor chip, including multiple sample test channels and non-specific reference channels; each sample test channel passes through the hose 24 at the corresponding position. Different specific detection molecules are introduced to modify the sensing chip; the non-specific reference channel corresponds to the sensing membrane that is not modified with specific detection molecules.

Specifically, the SPR excitation module uses p-polarized light to excite the SPR effect of the sample to be measured and generates multi-channel reflected light with the SPR effect, and the multi-channel reflected light is incident on the multi-channel photoelectric detection module.

Referring to Figures 1 and 2, the SPR excitation module includes a semi-cylindrical prism bracket 23, a semi-cylindrical prism 5 and a sensor chip. The sensing chip includes a sensing film 7 and a glass sheet 6 . In a specific embodiment, the semi-cylindrical prism 5 is preferably a K9 semi-cylindrical prism, and the glass sheet 6 is a K9 glass sheet.

The semi-cylindrical prism 5 is installed in the semi-cylindrical prism bracket 23; the semi-cylindrical prism bracket 23 is cooperatively connected with the polarizing beam splitter prism bracket 18. The glass sheet 6 is located on the plane of the semi-cylindrical prism 5, and the semi-cylindrical prism 5 and the glass sheet 6 are coupled through a refractive index matching liquid; the sensing film 7 is arranged on the glass sheet 6. The glass sheet 6 is detachable. The material and thickness of the sensing film 7 are determined according to the type of sample to be tested through physical and chemical means, and the sensing film 7 is modified onto the glass sheet 6 . Among them, for different detection substances, the corresponding biomolecule film system is modified, and different samples to be tested are detected by replacing the corresponding sensing film 7 when the top cover 17 of the housing 13 is opened. For example, a gold film with a thickness of 50nm is commonly used in detection devices. The sensing film 7.

The p-polarized light that can excite the SPR effect is incident on the semi-cylindrical prism 5 and the sensor chip in sequence. The p-polarized light covers the sensor chip of multiple channels and excites the near-infrared SPR effect, causing the SPR evanescent field to radiate to the near field of the sensor chip. On the sample to be tested, the multi-channel reflected light with SPR absorption characteristics and refractive index information of the sample to be tested is detected by multiple indium gallium arsenic photodetectors after passing through the sensor chip and the semi-cylindrical prism 5 respectively.

As an embodiment, p-polarized light is incident obliquely on the sensor chip at a fixed angle, where the fixed angle is 62.77°-62.8°. The incident angle of p-polarized light determines the size of the resonance wavelength. Setting the incident angle to 62.77°-62.8° makes the sensitivity optimal when the resonance wavelength is slightly larger than the wavelength of the fiber laser transmitter.

Specifically, the multi-channel photoelectric detection module is used to detect the light intensity signals of the s-polarized light and the multi-channel reflected light and send them to the signal processing module.

The multi-channel photoelectric detection module of the present invention can detect multiple light intensity signals, including the light intensity signal of the light intensity self-compensation module and the multi-channel light intensity signal of the SPR excitation module. The multi-channel photodetection module includes a plurality of indium gallium arsenide photodetectors, and each indium gallium arsenide photodetector includes a light intensity reference channel detector 801, a plurality of sample test channel detectors and a non-specific reference channel detector 804. Among them, the light intensity reference channel detector 801 is installed in the polarization beam splitter prism bracket 18 and is located on the outgoing optical path of the s-polarized light, and is used to detect the light intensity signal of the s-polarized light. Multiple sample test channel detectors and non-specific reference channel detectors 804 are installed on the semi-cylindrical prism bracket 23 and are located on the outgoing optical path of the multi-channel reflected light.

As a specific embodiment, the partition on the cover plate 17 divides the sensing film 7 into two sample test channels (sample A test channel and sample B test channel) and a non-specific reference channel, a total of three channels. In the present invention, the non-specific sensing film is first modified on the glass sheet 6: the type A specific detection molecules are passed into the sample A test channel, the type B specific detection molecule film is passed into the sample B test channel, and the non-specific reference channel is Corresponds to the sensing membrane without specific detection molecular membrane. If multiple samples A and B are dissolved in the same sample, the samples can be passed through all channels at the same time. The sample A test channel detector 802, the sample B test channel detector 803 and the non-specific reference channel detector 804 are respectively set at the sample A test channel, the sample B test channel and the non-specific reference channel to detect the reflection of each channel. The light intensity signal of the light is compared and the light intensity signal obtained by the non-specific reference channel detector 804 is used as the control signal, then the concentrations of the sample A and the sample B can be obtained.

Specifically, the signal processing module is used to determine the refractive index and concentration of the sample to be measured based on the light intensity signal and send it to the result display module.

Referring to Figures 1 and 2, the signal processing module includes a multi-channel signal converter 9, a microcontroller expansion board 20 and a microcontroller 10 connected in sequence. Among them, the multi-channel signal converter 9 is connected to the multi-channel photoelectric detection module, that is, the multi-channel signal converter 9 is connected to multiple indium gallium arsenide photodetectors through wires, and is used to convert multiple light intensity signals into multiple electrical signals. . The multi-channel electrical signals are transmitted to the single-chip computer 10 through the single-chip computer expansion board 20, that is, the multi-channel signal converter 9 is connected to the single-chip computer 10 and the single-chip computer expansion board 20 through wires respectively. Among them, the multi-channel signal converter 9 converts the multi-channel light intensity signals into multiple current signals, the single-chip computer expansion board 20 converts the current signals into voltage signals, and transfers the voltage signals to the single-chip computer 10; the single-chip computer 10 expands with the single-chip computer through pins The microcontroller expansion board 20 is connected to the multi-channel signal converter 9 through wires to receive the light intensity information collected by multiple indium gallium arsenic photodetectors in real time; and the microcontroller 10 is used to determine the corresponding light intensity based on the multi-channel voltage signals. information, and calculate the refractive index and concentration of various samples to be tested based on the light intensity information.

Specifically, the result display module is used to display the refractive index and concentration.

Figure 3 is a schematic diagram of the external structure of the micro analyzer provided by the present invention. As shown in Figure 3, the result display module includes a display screen 11; wherein the display screen 11 is a serial port LCD screen. The display screen 11 is located on the top of the housing 13 and is connected to the microcontroller 10 for displaying the refractive index and concentration of various samples to be measured.

As shown in Figure 3, the outer surface of the housing 13 of a micro analyzer based on the self-compensating near-infrared SPR effect provided by the present invention is provided with a heat dissipation hole 14, a power switch 15, a charging port 16, a USB port 21, a cover 17 and Display 11, in which USB port 21, cover 17 and display 11 are located on the top of housing 13, heat dissipation hole 14 is located on the front side of housing 13, charging port 16 and power switch 15 are located on one side of housing 13.

The interior of the micro analyzer is provided with a fiber laser transmitter 1, a power supply device 12, a multi-channel signal converter 9, and a microcontroller expansion board 20, which are connected and fixed to the shell 13 through screws. Among them, the power supply device 12 is connected to an external power supply through the charging port 16 for charging; and the power supply device 12 is connected to the laser emission module, the multi-channel photoelectric detection module, the signal processing module and the result display module through the power switch 15 to provide power. The microcontroller 10 transmits the calculated refractive index and concentration data of the sample to be measured to the display screen 11, and can save the data to the user's storage device through the USB port 21.

As an embodiment, the present invention uses a connecting bracket to fix each part of the optical elements, uses the transmission optical fiber 22 to connect the optical path, and integrates it into the internal structure of the instrument.

Figure 4 is a schematic diagram of the housing size of the micro analyzer provided by the present invention. As shown in Figure 4, the length of the housing 13 of the micro analyzer is a=12cm, the width is b=7cm, and the height is c=8cm. Compared with the existing A high-precision detection instrument, the invention effectively reduces the volume of the instrument and achieves both high precision and miniaturization.

When a micro-analyzer based on the self-compensating near-infrared SPR effect provided by the present invention detects the sample to be tested, the specific steps are as follows:

1) Turn on the power switch 15 and turn on the micro analyzer.

2) Open the cover 17 on the top of the housing 13, put a drop of refractive index matching liquid on the semi-cylindrical prism 5, and place the glass sheet 6 coated with a non-specific sensing film on the semi-cylindrical prism 5.

3) Close the cover 17 on the top of the housing 13, and pass the specific detection substance corresponding to the sample to be tested into the sensor chip through the hose 24 on the cover 17.

4) Drop the sample to be measured onto the sensor chip, or pass the sample to be measured onto the sensor chip through the hose 24 .

5) Click the confirmation button on the display screen 11. The display screen 11 displays the refractive index and concentration of the sample to be measured.

6) Insert the U disk into USB port 21 to store data.

7) After use, turn off the power switch 15 and take out the sensor chip for next use.

To sum up, compared with existing detection instruments on the market, the micro analyzer provided by the present invention has the following advantages:

(1) Compared with detection instruments with higher detection accuracy in the prior art, the micro-analyzer of the present invention uses a near-infrared light source. Since the laser wavelength used is in the C-band to stimulate the near-infrared SPR effect, the detection sensitivity is improved. The detection accuracy is greatly enhanced and online instant detection is achieved.

(2) The micro analyzer of the present invention adds a polarizing beam splitter prism 4, which greatly reduces the half-peak width of the incident light and improves the quality factor of the sensor.

(3) The micro-analyzer of the present invention uses an indium gallium arsenic photodetector instead of a spectrometer, a single-chip microcomputer 10 instead of a computer, a small display 11 instead of a traditional display screen, and a charging module instead of an online power supply, thereby reducing the size of the optical device. , realizing the portability and miniaturization of SPR sensing equipment, greatly reducing detection costs.

(4) Compared with the intensity modulation SPR device in the prior art, the micro analyzer of the present invention uses a self-compensation module and a multi-channel detection module to realize self-compensation of light source stability, thereby improving detection sensitivity.

(5) The micro-analyzer of the present invention uses a multi-channel detection module to detect different samples to be measured at the same time, and the light intensity signal of the reference channel can be used as a control signal to correct signal errors, saving time and cost, and greatly enhancing the intensity modulation SPR equipment. stability and sensitivity.

(6) The micro analyzer of the present invention uses a semi-cylindrical prism 5, which reduces the size of the instrument and the cost required to correct the incident light angle.

Each embodiment in this specification is described in a progressive manner. Each embodiment focuses on its differences from other embodiments. The same and similar parts between the various embodiments can be referred to each other.

This article uses specific examples to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method and the core idea of the present invention; at the same time, for those of ordinary skill in the art, according to the present invention There will be changes in the specific implementation methods and application scope of the ideas. In summary, the contents of this description should not be construed as limitations of the present invention.

Claims (10)

1. A self-compensating near infrared SPR effect based microanalyzer comprising: the device comprises a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module;

the laser emission module is used for emitting C-band laser and making the C-band laser incident to the light intensity self-compensation module;

the light intensity self-compensation module is used for dividing the C-band laser into s-polarized light with the non-excitable SPR effect and p-polarized light with the excitable SPR effect, and making the p-polarized light incident to the SPR excitation module and the s-polarized light incident to the multi-path photoelectric detection module;

the multichannel detection module comprises a plurality of sample test channels and a non-specific reference channel and is used for simultaneously detecting a plurality of samples to be detected;

the SPR excitation module excites an SPR effect of a sample to be detected by using p-polarized light, generates multipath channel reflected light with the SPR effect, and makes the multipath channel reflected light incident to the multipath photoelectric detection module;

the multipath photoelectric detection module is used for detecting the light intensity signals of the s polarized light and the multipath channel reflected light and sending the signals to the signal processing module;

the signal processing module is used for determining the refractive index and concentration of the sample to be tested according to the light intensity signal and sending the refractive index and concentration to the result display module;

the result display module is used for displaying the refractive index and the concentration.

2. The micro analyzer of claim 1, wherein the laser emitting module comprises: the device comprises an optical fiber laser transmitter, a transmission optical fiber, a C-band laser diode, an incident light optical fiber collimating mirror and a collimating mirror bracket;

the optical fiber laser transmitter is connected with the C-band laser diode through the transmission optical fiber; the optical fiber laser transmitter is used for generating laser and is incident to the C-band laser diode through the transmission optical fiber, and the C-band laser diode generates C-band laser;

the incident light optical fiber collimating mirror is arranged on the collimating mirror bracket; the collimating lens bracket is used for fixing the incident light optical fiber collimating lens on a light path of the C-band laser incident to the light intensity self-compensating module, and the incident light optical fiber collimating lens is used for collimating the C-band laser and expanding the beam diameter.

3. The micro analyzer of claim 2, wherein the light intensity self-compensation module comprises: a polarization beam splitter prism and a polarization beam splitter prism support;

the polarization beam splitting prism is arranged in the polarization beam splitting prism bracket; the polarization beam splitter prism support is connected with the collimating lens support in a matching way through threads;

the polarization splitting prism divides the C-band laser into s-polarized light of the non-excitable SPR effect and p-polarized light of the excitable SPR effect.

4. The micro analyzer of claim 3, wherein the SPR excitation module comprises: the sensing device comprises a semi-cylindrical prism bracket, a semi-cylindrical prism and a sensing chip; the sensing chip comprises a sensing film and a glass sheet;

the semi-cylindrical prism is arranged in the semi-cylindrical prism bracket; the semi-cylindrical prism support is connected with the polarization beam splitting prism support in a matched mode;

the glass sheet is positioned on the plane of the semi-cylindrical prism, and the semi-cylindrical prism is coupled with the glass sheet through refractive index matching liquid; the sensing film is disposed on the glass sheet.

5. The micro analyzer of claim 4, wherein the p-polarized light is obliquely incident to the sensor chip at a fixed angle of 62.77 ° -62.8 °.

6. The micro analyzer of claim 4, wherein the multi-channel detection module comprises: a cover plate, a hose and a hose plug arranged on the housing;

the cover plate is positioned at the top of the shell, and a plurality of subareas are arranged on the cover plate; the hose plug is provided with threads, and the hose is fixed on each partition of the cover plate through the hose plug;

the sensing chip is positioned under the cover plate, and each partition of the cover plate corresponds to a plurality of channels on the sensing chip, including a plurality of sample testing channels and a non-specific reference channel; each path of sample testing channel is introduced with different specificity detection molecules through corresponding hoses so as to modify the sensing chip; the non-specific reference channel corresponds to a sensing membrane that is not modified with a specific detection molecule.

7. The micro analyzer of claim 6, wherein the multi-path photo detection module comprises: a light intensity reference channel detector, a plurality of sample test channel detectors, and a non-specific reference channel detector;

the light intensity reference channel detector is arranged in the polarization beam splitting prism bracket and is positioned on an emergent light path of the s-polarized light and used for detecting a light intensity signal of the s-polarized light;

the plurality of sample test channel detectors and the non-specific reference channel detector are arranged on the semi-cylindrical prism support and are positioned on an emergent light path of the multipath channel reflected light.

8. The micro analyzer of claim 7, wherein the signal processing module comprises: the multi-channel signal converter, the singlechip expansion board and the singlechip are connected in sequence;

the multipath signal converter is connected with the multipath photoelectric detection module and is used for converting multipath light intensity signals into multipath electric signals; the multipath electric signals are transmitted to the singlechip through the singlechip expansion board;

the singlechip is used for calculating the refractive indexes and the concentrations of various samples to be measured according to the multipath electric signals.

9. The micro analyzer of claim 8, wherein the result display module comprises: a display screen;

the display screen is positioned at the top of the shell and connected with the singlechip and is used for displaying the refractive indexes and the concentrations of various samples to be tested.

10. The micro analyzer of claim 9, further comprising: the charging device comprises a power supply device, a charging port and a power switch;

the power supply device is positioned inside the shell; the charging port and the power switch are positioned at one side of the shell; the power supply device is connected with an external power supply through the charging port for charging; the power supply device is respectively connected with the laser emission module, the multipath photoelectric detection module, the signal processing module and the result display module through the power switch to supply power.