CN116840194A - Micro analyzer based on self-compensating near infrared SPR effect - Google Patents
Micro analyzer based on self-compensating near infrared SPR effect Download PDFInfo
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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Abstract
The invention discloses a micro analyzer based on a self-compensation near infrared SPR effect, and relates to the technical field of SPR sensing. The micro analyzer comprises a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module; the laser emission module emits C-band laser; the light intensity self-compensating module divides the C-band laser into s polarized light of the non-excitable SPR effect and p polarized light of the excitable SPR effect; the multi-channel detection module detects a plurality of samples to be detected simultaneously; the SPR excitation module generates multipath channel reflected light with an SPR effect; the multi-path photoelectric detection module detects light intensity signals of s polarized light and reflected light of the multi-path channel; the signal processing module determines the refractive index and concentration of the sample to be tested according to the light intensity signal; the result display module displays the refractive index and the concentration. The invention can realize high sensitivity, high precision and rapid detection and simultaneously reduce the cost and the volume of the instrument.
Description
Technical Field
The invention relates to the technical field of SPR sensing, in particular to a micro analyzer based on a self-compensation near infrared SPR effect.
Background
SPR (surface plasmon resonance) technology is an important biological analysis technology that enables the real-time detection of interactions between biomolecules. The SPR technology is based on adsorption of biomolecules on a metal surface, utilizes the change of reflected light intensity caused by surface plasma waves generated by laser irradiation to detect interaction among the biomolecules, and is widely applied to the fields of drug screening, biosensing, biomedical research and the like.
The high-performance SPR analyzers currently on the market are large in general size and high in cost, and the popularization of the SPR analyzers is limited.
Disclosure of Invention
The invention aims to provide a micro analyzer based on a self-compensating near infrared SPR effect, so that high sensitivity, high precision and rapid detection are realized, and meanwhile, the cost and the volume of the analyzer are reduced.
In order to achieve the above object, the present invention provides the following solutions:
a self-compensating near infrared SPR effect based micro analyzer comprising: the device comprises a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module;
the laser emission module is used for emitting C-band laser and making the C-band laser incident to the light intensity self-compensation module;
the light intensity self-compensation module is used for dividing the C-band laser into s-polarized light with the non-excitable SPR effect and p-polarized light with the excitable SPR effect, and making the p-polarized light incident to the SPR excitation module and the s-polarized light incident to the multi-path photoelectric detection module;
the multichannel detection module comprises a plurality of sample test channels and a non-specific reference channel and is used for simultaneously detecting a plurality of samples to be detected;
the SPR excitation module excites an SPR effect of a sample to be detected by using p-polarized light, generates multipath channel reflected light with the SPR effect, and makes the multipath channel reflected light incident to the multipath photoelectric detection module;
the multipath photoelectric detection module is used for detecting the light intensity signals of the s polarized light and the multipath channel reflected light and sending the signals to the signal processing module;
the signal processing module is used for determining the refractive index and concentration of the sample to be tested according to the light intensity signal and sending the refractive index and concentration to the result display module;
the result display module is used for displaying the refractive index and the concentration.
Optionally, the laser emitting module includes: the device comprises an optical fiber laser transmitter, a transmission optical fiber, a C-band laser diode, an incident light optical fiber collimating mirror and a collimating mirror bracket;
the optical fiber laser transmitter is connected with the C-band laser diode through the transmission optical fiber; the optical fiber laser transmitter is used for generating laser and is incident to the C-band laser diode through the transmission optical fiber, and the C-band laser diode generates C-band laser;
the incident light optical fiber collimating mirror is arranged on the collimating mirror bracket; the collimating lens bracket is used for fixing the incident light optical fiber collimating lens on a light path of the C-band laser incident to the light intensity self-compensating module, and the incident light optical fiber collimating lens is used for collimating the C-band laser and expanding the beam diameter.
Optionally, the light intensity self-compensating module includes: a polarization beam splitter prism and a polarization beam splitter prism support;
the polarization beam splitting prism is arranged in the polarization beam splitting prism bracket; the polarization beam splitter prism support is connected with the collimating lens support in a matching way through threads;
the polarization splitting prism divides the C-band laser into s-polarized light of the non-excitable SPR effect and p-polarized light of the excitable SPR effect.
Optionally, the SPR excitation module comprises: the sensing device comprises a semi-cylindrical prism bracket, a semi-cylindrical prism and a sensing chip; the sensing chip comprises a sensing film and a glass sheet;
the semi-cylindrical prism is arranged in the semi-cylindrical prism bracket; the semi-cylindrical prism support is connected with the polarization beam splitting prism support in a matched mode;
the glass sheet is positioned on the plane of the semi-cylindrical prism, and the semi-cylindrical prism is coupled with the glass sheet through refractive index matching liquid; the sensing film is disposed on the glass sheet.
Optionally, the p-polarized light is obliquely incident to the sensor chip at a fixed angle, and the fixed angle is 62.77 ° -62.8 °.
Optionally, the multi-channel detection module includes: a cover plate, a hose and a hose plug arranged on the housing;
the cover plate is positioned at the top of the shell, and a plurality of subareas are arranged on the cover plate; the hose plug is provided with threads, and the hose is fixed on each partition of the cover plate through the hose plug;
the sensing chip is positioned under the cover plate, and each partition of the cover plate corresponds to a plurality of channels on the sensing chip, including a plurality of sample testing channels and a non-specific reference channel; each path of sample testing channel is introduced with different specificity detection molecules through corresponding hoses so as to modify the sensing chip; the non-specific reference channel corresponds to a sensing membrane that is not modified with a specific detection molecule.
Optionally, the multi-path photodetection module includes: a light intensity reference channel detector, a plurality of sample test channel detectors, and a non-specific reference channel detector;
the light intensity reference channel detector is arranged in the polarization beam splitting prism bracket and is positioned on an emergent light path of the s-polarized light and used for detecting a light intensity signal of the s-polarized light;
the plurality of sample test channel detectors and the non-specific reference channel detector are arranged on the semi-cylindrical prism support and are positioned on an emergent light path of the multipath channel reflected light.
Optionally, the signal processing module includes: the multi-channel signal converter, the singlechip expansion board and the singlechip are connected in sequence;
the multipath signal converter is connected with the multipath photoelectric detection module and is used for converting multipath light intensity signals into multipath electric signals; the multipath electric signals are transmitted to the singlechip through the singlechip expansion board;
the singlechip is used for calculating the refractive indexes and the concentrations of various samples to be measured according to the multipath electric signals.
Optionally, the result display module includes: a display screen;
the display screen is positioned at the top of the shell and connected with the singlechip and is used for displaying the refractive indexes and the concentrations of various samples to be tested.
Optionally, the micro analyzer further comprises: the charging device comprises a power supply device, a charging port and a power switch;
the power supply device is positioned inside the shell; the charging port and the power switch are positioned at one side of the shell; the power supply device is connected with an external power supply through the charging port for charging; the power supply device is respectively connected with the laser emission module, the multipath photoelectric detection module, the signal processing module and the result display module through the power switch to supply power.
According to the specific embodiment provided by the invention, the invention discloses the following technical effects:
the invention provides a micro analyzer based on self-compensation near infrared SPR effect, which comprises a laser emitting module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module. The laser emission module is used for emitting C-band laser and making the C-band laser incident to the light intensity self-compensation module; the light intensity self-compensation module is used for dividing the C-band laser into s-polarized light of the non-excitable SPR effect and p-polarized light of the excitable SPR effect, and the p-polarized light is incident to the SPR excitation module, and the s-polarized light is incident to the multi-path photoelectric detection module; the multichannel detection module comprises a plurality of sample test channels and a non-specific reference channel and is used for simultaneously detecting a plurality of samples to be detected; the SPR excitation module excites an SPR effect of a sample to be detected by using p-polarized light, generates multipath channel reflected light with the SPR effect, and makes the multipath channel reflected light incident to the multipath photoelectric detection module; the multi-path photoelectric detection module is used for detecting light intensity signals of the s-polarized light and the reflected light of the multi-path channel and sending the light intensity signals to the signal processing module; the signal processing module is used for determining the refractive index and concentration of the sample to be tested according to the light intensity signal and sending the refractive index and concentration to the result display module; and the result display module is used for displaying the refractive index and the concentration. The micro analyzer based on the self-compensation near infrared SPR effect realizes the detection of the concentration of a sample to be detected, and reduces the volume of the device on the basis of ensuring the sensing performance compared with the existing detection device using a spectrometer; in addition, the invention provides a plurality of test channels, which can detect a plurality of objects to be detected at the same time; the self-compensation module and the reference channel provided by the invention greatly improve the sensitivity and stability of detection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of a micro analyzer according to the present invention;
FIG. 2 is a schematic view of the front and back sides of a sensing area and an enlarged schematic view of a sensing film area of a micro analyzer according to the present invention;
FIG. 3 is a schematic view of the external structure of the micro analyzer according to the present invention;
fig. 4 is a schematic diagram of the dimensions of the housing of the micro analyzer according to the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The popularity of SPR analyzers is limited to a certain extent due to the large volume and high cost of high-performance SPR analyzers currently on the market. Therefore, research and development of the SPR analyzer with both miniaturization and high sensitivity can effectively reduce the cost and the volume of the analyzer while realizing high-sensitivity, high-precision and rapid detection, and has very important significance. The invention aims to provide a micro analyzer based on a self-compensating near infrared SPR effect, so that high sensitivity, high precision and rapid detection are realized, and meanwhile, the cost and the volume of the analyzer are reduced. The micro analyzer can be used for rapid and accurate detection of biomolecules and has the advantages of low cost, miniaturization, high precision, convenient use and the like.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Fig. 1 is a schematic diagram of the overall structure of a micro analyzer based on the self-compensating near-infrared SPR effect, and fig. 2 is a schematic diagram of the front and back surfaces of a sensing area and an enlarged schematic diagram of a sensing film area of the micro analyzer, as shown in fig. 1 and 2, the micro analyzer based on the self-compensating near-infrared SPR effect, provided by the invention, comprises: the device comprises a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module.
The laser emission module is used for emitting C-band laser and making the C-band laser incident to the light intensity self-compensation module.
Specifically, the laser emission module includes: a fiber laser transmitter 1, a transmission fiber 22, a C-band laser diode 2, an incident light fiber collimator 3 and a collimator lens bracket 19.
As shown in fig. 1 and 2, the fiber laser transmitter 1 is connected to a C-band laser diode 2 through a transmission fiber 22. The fiber laser transmitter 1 supplies power to the C-band laser diode 2, and is used for generating laser and adjusting the laser intensity, the generated laser is incident to the C-band laser diode 2 through the transmission fiber 22, and the C-band laser is generated by the C-band laser diode 2.
The incident light optical fiber collimating mirror 3 is arranged on the collimating mirror bracket 19; the incident light optical fiber collimating mirror 3 is connected with the collimating mirror bracket 19 in a matching way through threads, the collimating mirror bracket 19 is used for fixing the incident light optical fiber collimating mirror 3 on a light path of C-band laser incident to the light intensity self-compensating module, and is used for collimating the C-band laser and expanding the beam diameter, so that the C-band laser can cover the whole sensing chip.
The light intensity self-compensation module is used for dividing the C-band laser into s-polarized light with the non-excitable SPR effect and p-polarized light with the excitable SPR effect, and the p-polarized light is incident to the SPR excitation module, and the s-polarized light is incident to the multi-path photoelectric detection module.
Specifically, the light intensity self-compensation module includes: a polarization splitting prism 4 and a polarization splitting prism holder 18. Wherein the polarization splitting prism 4 is arranged in the polarization splitting prism bracket 18; the polarization splitting prism support 18 is connected with the collimating lens support 19 in a matching way through threads.
The polarization splitting prism 4 splits the C-band laser light into s-polarized light of the non-excitable SPR effect and p-polarized light of the excitable SPR effect. Wherein the s-polarized light provides a reference signal for the detection signal to correct errors due to source fluctuations, etc. The p-polarized light exciting the SPR effect is correspondingly processed by referring to signals such as light intensity and fluctuation of the s-polarized light, so that errors of molecular detection signals caused by problems such as light source noise, chip preparation process and nonspecific adsorption can be reduced.
Specifically, the multi-channel detection module comprises a plurality of sample testing channels and a non-specific reference channel, and is used for simultaneously detecting a plurality of samples to be detected. The sensing channels of the present invention are divided into two types: one is a multi-channel sample test channel and the other is a non-specific reference channel. Multiple sample test channels are required to be arranged for simultaneously detecting multiple samples to be tested. Before use, each path of sample test channel is introduced with different specific detection molecules to modify the sensing chip; and the non-specific reference channel corresponds to a sensing chip of the metal sensing film which is not modified with the specific detection molecular film. The sensing chip of the multi-path sample test channel and the non-specific reference channel has the same parameters of the material, the gold film thickness and the like except the specific detection molecular film.
Referring to fig. 1 and 3, the multi-channel detection module includes: a cover 17, a hose 24 and a hose plug 25 arranged on the housing 13. Wherein the cover 17 is located on top of the housing 13 and a plurality of partitions (3 partitions are shown in fig. 3) are provided on the cover 17. The partitions on the cover 17 divide the sensing membrane 7 into a plurality of sample test channels and non-specific reference channels for simultaneously detecting a plurality of samples to be detected and providing reference signals for the detection signals to correct the results.
The hose stopper 25 is provided with threads, and the hose 24 is fastened to the respective partitions of the cover 17 by the hose stopper 25. The sensing chip is positioned under the cover plate 17, and each partition of the cover plate 17 corresponds to a plurality of channels on the sensing chip, including a plurality of sample testing channels and a non-specific reference channel; each path of sample testing channel is introduced with different specificity detection molecules through a hose 24 at a corresponding position so as to modify the sensing chip; the non-specific reference channel corresponds to a sensing membrane that is not modified with a specific detection molecule.
Specifically, the SPR excitation module excites the SPR effect of the sample to be detected by using p-polarized light, generates multipath channel reflected light with the SPR effect, and makes the multipath channel reflected light incident to the multipath photoelectric detection module.
Referring to fig. 1 and 2, the SPR excitation module includes a half-cylinder prism support 23, a half-cylinder prism 5, and a sensing chip. The sensor chip comprises a sensor film 7 and a glass sheet 6. In one embodiment, the semi-cylindrical prism 5 is preferably a K9 semi-cylindrical prism and the glass sheet 6 is a K9 glass sheet.
The semi-cylindrical prism 5 is installed in the semi-cylindrical prism support 23; the semi-cylindrical prism support 23 is cooperatively connected with the polarization splitting prism support 18. The glass sheet 6 is positioned on the plane of the semi-cylindrical prism 5, and the semi-cylindrical prism 5 is coupled with the glass sheet 6 through refractive index matching liquid; the sensing film 7 is provided on the glass sheet 6. The glass sheet 6 is detachable, the material and thickness of the sensing film 7 are determined according to the type of the sample to be measured through physical and chemical modes, and the sensing film 7 is decorated on the glass sheet 6. Wherein, for different detection substances, the corresponding bio-molecular film system is modified, and different samples to be detected are detected by replacing the corresponding sensing film 7 when the top cover plate 17 of the housing 13 is opened, for example, a gold film with the thickness of 50nm is used as the sensing film 7 commonly used by the detection device.
P polarized light capable of exciting an SPR effect is sequentially incident to the semi-cylindrical prism 5 and the sensing chip, p polarized light covers the sensing chips of the channels and excites a near infrared SPR effect, so that an SPR evanescent field is radiated to a sample to be detected in the near field of the sensing chip, and then multipath channel reflected light with SPR absorption characteristics and refractive index information of the sample to be detected is sequentially detected by a plurality of InGaAs photodetectors after passing through the sensing chip and the semi-cylindrical prism 5 respectively.
As an example, p-polarized light is obliquely incident to the sensor chip at a fixed angle, wherein the fixed angle is 62.77 ° -62.8 °. The incidence angle of p-polarized light determines the magnitude of the resonant wavelength, and setting the incidence angle to 62.77 ° -62.8 ° allows the resonant wavelength to be slightly greater than the fiber laser transmitter wavelength, with optimal sensitivity.
Specifically, the multi-path photoelectric detection module is used for detecting the light intensity signals of the s-polarized light and the light reflected by the multi-path channel and sending the signals to the signal processing module.
The multi-path photoelectric detection module can detect multi-path light intensity signals, and comprises a light intensity signal of a light intensity self-compensation module and multi-path light intensity signals of an SPR excitation module. The multi-channel photodetector module comprises a plurality of InGaAs photodetectors, each of which comprises a light intensity reference channel detector 801, a plurality of sample test channel detectors, and a non-specific reference channel detector 804. The light intensity reference channel detector 801 is installed in the polarization beam splitter prism support 18 and is located on the outgoing light path of the s polarized light, and is used for detecting the light intensity signal of the s polarized light. A plurality of sample test channel detectors and a non-specific reference channel detector 804 are mounted on the semi-cylindrical prism support 23 and are positioned on the outgoing light path of the multi-channel reflected light.
As a specific example, the partition on the cover 17 divides the sensing membrane 7 into two sample test channels (sample a test channel and sample B test channel) and a nonspecific reference channel. The invention first modifies the nonspecific sensing membrane on the glass sheet 6: and (3) introducing the class A specific detection molecules into a sample A test channel, introducing the class B specific detection molecule film into a sample B test channel, and enabling the non-specific reference channel to correspond to the sensing film without the specific detection molecule film. If multiple samples A and B to be tested are dissolved in the same sample to be tested, the samples to be tested can be simultaneously introduced into all channels. The sample a test channel detector 802, the sample B test channel detector 803 and the non-specific reference channel detector 804 are correspondingly arranged at the sample a test channel, the sample B test channel and the non-specific reference channel, and are used for detecting and comparing light intensity signals of reflected light of all channels, and the concentrations of the sample a to be detected and the sample B to be detected can be obtained by taking the light intensity signals obtained by the non-specific reference channel detector 804 as control signals.
Specifically, the signal processing module is used for determining the refractive index and concentration of the sample to be tested according to the light intensity signal and sending the refractive index and concentration to the result display module.
Referring to fig. 1 and 2, the signal processing module includes a multi-channel signal converter 9, a singlechip expansion board 20, and a singlechip 10, which are sequentially connected. The multi-channel signal converter 9 is connected with the multi-channel photoelectric detection module, namely, the multi-channel signal converter 9 is connected with a plurality of InGaAs photoelectric detectors through wires and is used for converting multi-channel light intensity signals into multi-channel electric signals. The multipath electric signals are transmitted to the singlechip 10 through the singlechip expansion board 20, namely the multipath signal converter 9 is respectively connected with the singlechip 10 and the singlechip expansion board 20 through wires. The multi-channel signal converter 9 converts the multi-channel light intensity signals into multi-channel current signals, the singlechip expansion board 20 converts the current signals into voltage signals, and the voltage signals are transferred to the singlechip 10; the singlechip 10 is connected with the singlechip expansion board 20 through pins, the singlechip expansion board 20 is connected with the multipath signal converter 9 through wires, and light intensity information acquired by a plurality of InGaAs photoelectric detectors is received in real time; and the singlechip 10 is used for determining corresponding light intensity information according to the multipath voltage signals, and calculating the refractive indexes and the concentrations of various samples to be measured through the light intensity information.
Specifically, the result display module is used for displaying the refractive index and the concentration.
FIG. 3 is a schematic diagram of the external structure of the micro analyzer provided by the present invention, as shown in FIG. 3, the result display module includes a display screen 11; the display screen 11 is a serial liquid crystal screen. The display screen 11 is located at the top of the housing 13 and connected with the single chip microcomputer 10, and is used for displaying refractive indexes and concentrations of various samples to be tested.
As shown in fig. 3, the outer surface of a housing 13 of the micro analyzer based on the self-compensating near infrared SPR effect provided by the invention is provided with a heat dissipation hole 14, a power switch 15, a charging hole 16, a USB hole 21, a cover plate 17 and a display screen 11, wherein the USB hole 21, the cover plate 17 and the display screen 11 are positioned at the top of the housing 13, the heat dissipation hole 14 is positioned at the front side of the housing 13, and the charging hole 16 and the power switch 15 are positioned at one side of the housing 13.
The micro analyzer is internally provided with an optical fiber laser transmitter 1, a power supply device 12, a multipath signal converter 9 and a singlechip expansion board 20 which are respectively connected and fixed with a shell 13 through screws. Wherein, the power supply device 12 is connected with an external power supply through a charging port 16 for charging; and the power supply device 12 is respectively connected with the laser emitting module, the multipath photoelectric detection module, the signal processing module and the result display module through the power switch 15 for supplying power. The singlechip 10 transmits the refractive index and concentration data of the sample to be measured obtained after calculation to the display screen 11, and can store the data on storage equipment of a user through the USB port 21.
As an embodiment, the present invention uses a connection bracket to secure the optical components of each part, and uses a transmission fiber 22 to make optical path connection, and is integrated into the internal structure of the instrument.
Fig. 4 is a schematic diagram of the size of the shell of the micro analyzer provided by the invention, as shown in fig. 4, the shell 13 of the micro analyzer has a length of a=12 cm, a width of b=7cm, and a height of c=8cm.
The invention provides a self-compensating near infrared SPR effect-based micro analyzer, which comprises the following specific use steps when detecting a sample to be detected:
1) The power switch 15 is turned on to turn on the micro analyzer.
2) The cover 17 on top of the housing 13 is opened, a drop of index matching liquid is dropped onto the semi-cylindrical prism 5, and the glass sheet 6 coated with the nonspecific sensing film is placed on the semi-cylindrical prism 5.
3) Closing the cover plate 17 on the top of the shell 13, and introducing the specific detection substances corresponding to the sample to be detected into the sensing chip through the hose 24 on the cover plate 17.
4) The sample to be measured is dropped onto the sensor chip or is passed through the hose 24 onto the sensor chip.
5) Clicking a confirm button on the display screen 11, the display screen 11 displays the refractive index and the concentration of the sample to be measured.
6) The USB flash disk is inserted into the USB port 21 to store data.
7) After the use, the power switch 15 is turned off, and the sensor chip is taken out for the next use.
In summary, compared with the existing detection instrument in the market, the micro analyzer provided by the invention has the following advantages:
(1) Compared with the detection instrument with higher detection precision in the prior art, the micro analyzer provided by the invention adopts the near infrared light source, and the used laser wavelength excites the near infrared SPR effect in the C wave band, so that the detection sensitivity is improved, the detection precision is greatly enhanced, and the online instant detection is realized.
(2) The micro analyzer of the invention is added with the polarization beam splitter prism 4, thereby greatly reducing the half-peak width of incident light and improving the quality factor of the sensor.
(3) The micro analyzer of the invention adopts the InGaAs photoelectric detector to replace a spectrometer, adopts the singlechip 10 to replace a computer, adopts the small display screen 11 to replace a traditional display screen, adopts the charging module to replace an on-line power supply, reduces the volume of an optical device, realizes the portability and miniaturization of SPR sensing equipment, and greatly reduces the detection cost.
(4) Compared with the intensity modulation type SPR device in the prior art, the micro analyzer adopts the self-compensation module and the multi-channel detection module to realize the self-compensation of the stability of the light source, so that the detection sensitivity is improved.
(5) The micro analyzer can detect different samples to be detected simultaneously by using the multi-channel detection module, and the light intensity signal of the reference channel can be used as a comparison signal to correct signal errors, so that the time cost is saved, and the stability and the sensitivity of the intensity modulation SPR equipment are greatly enhanced.
(6) The micro analyzer of the invention adopts the semi-cylindrical prism 5, thereby reducing the volume of the instrument and the cost for correcting the angle of incident light.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present invention and the core ideas thereof; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (10)
1. A self-compensating near infrared SPR effect based microanalyzer comprising: the device comprises a laser emission module, a light intensity self-compensation module, a multi-channel detection module, an SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module;
the laser emission module is used for emitting C-band laser and making the C-band laser incident to the light intensity self-compensation module;
the light intensity self-compensation module is used for dividing the C-band laser into s-polarized light with the non-excitable SPR effect and p-polarized light with the excitable SPR effect, and making the p-polarized light incident to the SPR excitation module and the s-polarized light incident to the multi-path photoelectric detection module;
the multichannel detection module comprises a plurality of sample test channels and a non-specific reference channel and is used for simultaneously detecting a plurality of samples to be detected;
the SPR excitation module excites an SPR effect of a sample to be detected by using p-polarized light, generates multipath channel reflected light with the SPR effect, and makes the multipath channel reflected light incident to the multipath photoelectric detection module;
the multipath photoelectric detection module is used for detecting the light intensity signals of the s polarized light and the multipath channel reflected light and sending the signals to the signal processing module;
the signal processing module is used for determining the refractive index and concentration of the sample to be tested according to the light intensity signal and sending the refractive index and concentration to the result display module;
the result display module is used for displaying the refractive index and the concentration.
2. The micro analyzer of claim 1, wherein the laser emitting module comprises: the device comprises an optical fiber laser transmitter, a transmission optical fiber, a C-band laser diode, an incident light optical fiber collimating mirror and a collimating mirror bracket;
the optical fiber laser transmitter is connected with the C-band laser diode through the transmission optical fiber; the optical fiber laser transmitter is used for generating laser and is incident to the C-band laser diode through the transmission optical fiber, and the C-band laser diode generates C-band laser;
the incident light optical fiber collimating mirror is arranged on the collimating mirror bracket; the collimating lens bracket is used for fixing the incident light optical fiber collimating lens on a light path of the C-band laser incident to the light intensity self-compensating module, and the incident light optical fiber collimating lens is used for collimating the C-band laser and expanding the beam diameter.
3. The micro analyzer of claim 2, wherein the light intensity self-compensation module comprises: a polarization beam splitter prism and a polarization beam splitter prism support;
the polarization beam splitting prism is arranged in the polarization beam splitting prism bracket; the polarization beam splitter prism support is connected with the collimating lens support in a matching way through threads;
the polarization splitting prism divides the C-band laser into s-polarized light of the non-excitable SPR effect and p-polarized light of the excitable SPR effect.
4. The micro analyzer of claim 3, wherein the SPR excitation module comprises: the sensing device comprises a semi-cylindrical prism bracket, a semi-cylindrical prism and a sensing chip; the sensing chip comprises a sensing film and a glass sheet;
the semi-cylindrical prism is arranged in the semi-cylindrical prism bracket; the semi-cylindrical prism support is connected with the polarization beam splitting prism support in a matched mode;
the glass sheet is positioned on the plane of the semi-cylindrical prism, and the semi-cylindrical prism is coupled with the glass sheet through refractive index matching liquid; the sensing film is disposed on the glass sheet.
5. The micro analyzer of claim 4, wherein the p-polarized light is obliquely incident to the sensor chip at a fixed angle of 62.77 ° -62.8 °.
6. The micro analyzer of claim 4, wherein the multi-channel detection module comprises: a cover plate, a hose and a hose plug arranged on the housing;
the cover plate is positioned at the top of the shell, and a plurality of subareas are arranged on the cover plate; the hose plug is provided with threads, and the hose is fixed on each partition of the cover plate through the hose plug;
the sensing chip is positioned under the cover plate, and each partition of the cover plate corresponds to a plurality of channels on the sensing chip, including a plurality of sample testing channels and a non-specific reference channel; each path of sample testing channel is introduced with different specificity detection molecules through corresponding hoses so as to modify the sensing chip; the non-specific reference channel corresponds to a sensing membrane that is not modified with a specific detection molecule.
7. The micro analyzer of claim 6, wherein the multi-path photo detection module comprises: a light intensity reference channel detector, a plurality of sample test channel detectors, and a non-specific reference channel detector;
the light intensity reference channel detector is arranged in the polarization beam splitting prism bracket and is positioned on an emergent light path of the s-polarized light and used for detecting a light intensity signal of the s-polarized light;
the plurality of sample test channel detectors and the non-specific reference channel detector are arranged on the semi-cylindrical prism support and are positioned on an emergent light path of the multipath channel reflected light.
8. The micro analyzer of claim 7, wherein the signal processing module comprises: the multi-channel signal converter, the singlechip expansion board and the singlechip are connected in sequence;
the multipath signal converter is connected with the multipath photoelectric detection module and is used for converting multipath light intensity signals into multipath electric signals; the multipath electric signals are transmitted to the singlechip through the singlechip expansion board;
the singlechip is used for calculating the refractive indexes and the concentrations of various samples to be measured according to the multipath electric signals.
9. The micro analyzer of claim 8, wherein the result display module comprises: a display screen;
the display screen is positioned at the top of the shell and connected with the singlechip and is used for displaying the refractive indexes and the concentrations of various samples to be tested.
10. The micro analyzer of claim 9, further comprising: the charging device comprises a power supply device, a charging port and a power switch;
the power supply device is positioned inside the shell; the charging port and the power switch are positioned at one side of the shell; the power supply device is connected with an external power supply through the charging port for charging; the power supply device is respectively connected with the laser emission module, the multipath photoelectric detection module, the signal processing module and the result display module through the power switch to supply power.
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Cited By (1)
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| CN117705765A (en) * | 2023-12-12 | 2024-03-15 | 大连海事大学 | Near infrared SPR integrated sensing device with high-performance multichannel |
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| CN114152569A (en) * | 2021-11-17 | 2022-03-08 | 北京英柏生物科技有限公司 | Imaging optical system based on SPR angular spectrum |
| CN115524312A (en) * | 2022-10-17 | 2022-12-27 | 大连海事大学 | Portable near-infrared SPR detection device |
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| CN107703103A (en) * | 2017-09-01 | 2018-02-16 | 苏州优函信息科技有限公司 | For detecting the ghSPR sensors and detection method of refractive index |
| CN114152569A (en) * | 2021-11-17 | 2022-03-08 | 北京英柏生物科技有限公司 | Imaging optical system based on SPR angular spectrum |
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