CN116830967A - Cordyceps militaris cultivation medium - Google Patents
Cordyceps militaris cultivation medium Download PDFInfo
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- CN116830967A CN116830967A CN202311025886.0A CN202311025886A CN116830967A CN 116830967 A CN116830967 A CN 116830967A CN 202311025886 A CN202311025886 A CN 202311025886A CN 116830967 A CN116830967 A CN 116830967A
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 38
- 240000006394 Sorghum bicolor Species 0.000 claims abstract description 38
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims abstract description 38
- 229930006000 Sucrose Natural products 0.000 claims abstract description 25
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 25
- 239000005720 sucrose Substances 0.000 claims abstract description 25
- 240000008042 Zea mays Species 0.000 claims abstract description 24
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 24
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 24
- 235000005822 corn Nutrition 0.000 claims abstract description 24
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 23
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- 235000013312 flour Nutrition 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 18
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- 235000012054 meals Nutrition 0.000 claims abstract description 7
- 239000000758 substrate Substances 0.000 claims description 18
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 10
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 4
- 230000012010 growth Effects 0.000 abstract description 16
- 239000002609 medium Substances 0.000 abstract description 10
- 238000012360 testing method Methods 0.000 description 13
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 11
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 10
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 9
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
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- 230000000694 effects Effects 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 241000190633 Cordyceps Species 0.000 description 4
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- 239000000126 substance Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 3
- 238000000034 method Methods 0.000 description 3
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 229930003270 Vitamin B Natural products 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
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- 239000011573 trace mineral Substances 0.000 description 2
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- 241000208140 Acer Species 0.000 description 1
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- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
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- 206010039509 Scab Diseases 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
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- 208000019902 chronic diarrheal disease Diseases 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 235000001705 insufficient nutrition Nutrition 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 235000021395 porridge Nutrition 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- MYVIATVLJGTBFV-UHFFFAOYSA-M thiamine(1+) chloride Chemical compound [Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N MYVIATVLJGTBFV-UHFFFAOYSA-M 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to a cultivation medium for cordyceps militaris. A culture medium for cordyceps militaris, comprising: sorghum, soybean meal, corn meal, sucrose, yeast, and vitamins. According to the cultivation medium for the cordyceps militaris, sorghum is used as a medium, and the cultivation medium is compounded with soybean powder, corn flour, sucrose, yeast and vitamins, so that the cost is reduced, the requirement of good growth of cordyceps militaris is fully met, and the quality of the cordyceps militaris is improved.
Description
Technical Field
The invention belongs to the technical field of cordyceps militaris, and particularly relates to a cordyceps militaris cultivation medium.
Background
Cordycepin (3' -deoxyadenosine): antiviral, antibacterial, and can obviously inhibit tumor growth and interfere with RNA and DNA synthesis of human body. Especially, among the Cordyceps species, cordycepin having the most characteristic representative component can be produced, and Cordyceps militaris is the only variety capable of producing a large amount of cordycepin. The cordycepin content in Cordyceps militaris is about 20 times that of natural Cordyceps militaris.
The Cordyceps militaris is based on artificial nutrition, and the different matrixes do not influence the formation of final substances of the Cordyceps militaris, wherein the cordycepin content is used as the main component. No matter what substrate the cordyceps sinensis grows on, the cordyceps sinensis is decomposed to become a nutrition source for the growth of the cordyceps sinensis and generate cordyceps sinensis fruiting bodies.
The sorghum contains more fat and iron than rice. The main nutritional ingredients of the sorghum are 9% of crude protein, 3.3% of crude fat, 85% of carbohydrate, 1% of crude fiber, and trace elements such as calcium, phosphorus, iron and the like and vitamin B groups according to dry matters. The sorghum has certain efficacy, and has the effects of harmonizing stomach, strengthening spleen, removing food retention, warming spleen and stomach, astringing intestines and stomach and stopping cholera. The sorghum contains tannin, has the functions of astringing and relieving depletion, and has obvious curative effect when patients suffering from chronic diarrhea frequently eat sorghum rice porridge. The protein in the sorghum is a incomplete protein which is more alcohol-soluble protein, has fewer amino acids necessary for human bodies such as tryptophan, lysine and the like, and is not easy to be absorbed by human bodies. If it is mixed with other grains, the nutritive value can be improved. The white sorghum rice is best to eat, contains the least tannins, contains the most cutin, has good eating quality, is ground into powder and is used as starch, and the flour quality is better. The sorghum is not only directly eaten, but also can be used for preparing sugar and wine. Gao Lianggen it is also indicated for asthma, diuresis and hemostasis. In grains, the sorghum protein has the lowest lysine content, and thus the protein quality is also the worst; gao Liangde the content of niacin is not as high as that of corn, but can be absorbed by human body, so that the region taking sorghum as main food rarely suffers from scab.
Based on the method, the invention provides a novel cordyceps militaris cultivation medium which contains sorghum components, so that the cost is reduced and the growth condition of cordyceps militaris is ensured.
Disclosure of Invention
The invention provides a cultivation medium for cordyceps militaris, which adopts sorghum as a substrate, is compounded with soybean powder, corn flour, sucrose, yeast and vitamins based on the special advantages of being rich in calcium, iron, vitamin B and the like, and can fully meet the requirement of good growth of cordyceps militaris and realize good growth condition of cordyceps militaris while reducing the cost.
In order to achieve the above purpose, the technical scheme adopted is as follows:
a culture medium for cordyceps militaris, comprising: sorghum, soybean meal, corn meal, sucrose, yeast, and vitamins.
Further, the vitamin is vitamin b1.
Further, the culture medium comprises the following raw materials in parts by weight: 0.5-2.0 parts of soybean powder, 0.1-1.6 parts of corn flour, 0-0.75 parts of sucrose, 0.05-0.07 parts of yeast, 0.002-0.004 parts of vitamin b1 and 27-33 parts of sorghum.
Still further, the cultivation matrix comprises the following raw materials in parts by weight: 0.5-2.0 parts of soybean powder, 0.1-1.6 parts of corn flour, 0-0.75 parts of sucrose, 0.061 parts of yeast, 0.0032 parts of vitamin b1 and 30 parts of sorghum.
Still further, the cultivation matrix comprises the following raw materials in parts by weight: 3.4 parts of soybean powder, 3.1 parts of corn flour, 0.9 part of sucrose, 0.19 part of yeast, 0.01 part of vitamin b1 and 92.4 parts of sorghum.
Compared with the prior art, the invention has the beneficial effects that:
1. the culture medium for cordyceps militaris adopts sorghum which is widely cultivated in China, northeast and Shaanxi are main production areas, and edible sorghum grains are adopted as the medium, so that the culture medium is easy to obtain and low in cost.
2. The key point of the cultivation of the cordyceps militaris is that the nutrition components, proportion, morphology and absorption conditions provided by the human body can meet the requirement of good growth of cordyceps militaris. The cultivation medium for the cordyceps militaris is beneficial to the production of cordyceps militaris by reasonable compounding.
3. The culture medium for cordyceps militaris has low cost, can meet the requirement of good growth of cordyceps militaris, and has good culture effect.
Detailed Description
In order to further illustrate a cultivation substrate for cordyceps militaris of the invention and achieve the purpose of the invention, the following is a detailed description of specific implementation, structure, characteristics and effects of the cultivation substrate for cordyceps militaris according to the invention in combination with the preferred embodiments. In the following description, different "an embodiment" or "an embodiment" do not necessarily refer to the same embodiment. Furthermore, the particular features, structures, or characteristics of one or more embodiments may be combined in any suitable manner.
The cultivation substrate of cordyceps militaris of the invention is described in further detail below by combining specific embodiments:
the raw materials used in the present invention are all common and commercially available in the art. Specific:
soybean powder is a food prepared by baking and pulverizing soybean. The soybean powder has the characteristics of high soybean protein content, high unsaturated fatty acid content and the like, has the health-care effects of resisting aging, strengthening brain and the like, and is food with high nutritional value.
Sucrose, which is a main component of sugar, is a kind of disaccharide, and is formed by condensing and dehydrating one molecule of glucose hemiacetal hydroxyl group and one molecule of fructose hemiacetal hydroxyl group with each other. Sucrose has sweet taste, no smell, is easily soluble in water and glycerol, and is slightly soluble in alcohol. Has optical rotation but no optical rotation change. Sucrose is almost universally present in leaves, flowers, stems, seeds, and fruits of the plant kingdom. The content of the sugar cane juice, the sugar beet juice and the maple juice is particularly rich. Sucrose taste sweet, is an important food and sweet flavoring.
Vitamins, a class of trace organic substances that humans and animals must obtain from food in order to maintain normal physiological functions, play an important role in the growth, metabolism, and development of the human body. Vitamins are not involved in the body in forming human cells nor in providing energy to the human body. Vitamins participate in biochemical reactions of human body and regulate metabolic functions of human body. The present invention is preferably vitamin b1.
Vitamin b1, also known as thiamine, is the water-soluble vitamin purified by people at the earliest and has the chemical name 3- [ (4-amino-2-methyl-5-pyrimidinyl) -methyl ] -5- (2-hydroxyethyl) -4-methylthiazolium chloride, and has the function of maintaining normal sugar metabolism.
The technical scheme of the invention is as follows:
a culture medium for cordyceps militaris, comprising: sorghum, soybean meal, corn meal, sucrose, yeast, and vitamins.
Single nitrogen sources exhibit differences in growth rate and fruiting body yield, such as single soybean flour as nitrogen source, fruiting body yield is higher but growth rate is slow. Therefore, the composite nitrogen source is adopted in the technical scheme of the invention, the composite nitrogen source is more beneficial to the growth of cordyceps militaris fruiting body, and the mycelium growth speed and fruiting body yield are both optimal by compounding. And soybean powder, corn flour and yeast are common nitrogen sources, so that the price is low and the soybean powder, corn flour and yeast are easy to obtain.
Preferably, the vitamin is vitamin b1.
Preferably, the culture medium comprises the following raw materials in parts by weight: 0.5-2.0 parts of soybean powder, 0.1-1.6 parts of corn flour, 0-0.75 parts of sucrose, 0.05-0.07 parts of yeast, 0.002-0.004 parts of vitamin b1 and 27-33 parts of sorghum.
Further preferably, the cultivation substrate comprises the following raw materials in parts by weight: 0.5-2.0 parts of soybean powder, 0.1-1.6 parts of corn flour, 0-0.75 parts of sucrose, 0.061 parts of yeast, 0.0032 parts of vitamin b1 and 30 parts of sorghum.
Further preferably, the cultivation substrate comprises the following raw materials in parts by weight: 3.4 parts of soybean powder, 3.1 parts of corn flour, 0.9 part of sucrose, 0.19 part of yeast, 0.01 part of vitamin b1 and 92.4 parts of sorghum.
Example 1.
The compound ingredients are shown in table 1.
TABLE 1
| Name of the name | Weight of (E) | Unit (B) | The ratio of% |
| Soybean powder | 1.1 | g | 3.4 |
| Corn flour | 1 | g | 3.1 |
| Sucrose | 0.3 | g | 0.9 |
| Yeast | 0.061 | g | 0.19 |
| Vitamin b1 | 0.0032 | g | 0.01 |
| (sorghum) | 30 | g | 92.4 |
Note that: when the sorghum is used, the cortex needs to be properly removed, and the white sorghum is better and can realize the eating of the substrate.
Example 2.
The cultivation substrate comprises: 1.1g of soybean meal, 1.0g of corn meal, 0.3g of sucrose, 0.05g of yeast, 0.002g of vitamin b1 and 27g of sorghum.
Example 3.
The cultivation substrate comprises: 1.1g of soybean meal, 1.0g of corn meal, 0.3g of sucrose, 0.07g of yeast, 0.004g of vitamin b1 and 33g of sorghum.
Example 4.
The cultivation substrate comprises: 1.2g of soybean meal, 0.8g of corn flour, 0.4g of sucrose, 0.065g of yeast, 0.003g of vitamin b1 and 31g of sorghum.
Example 5: experiment
(1) Test object: cordyceps militaris
The test group uses sorghum as a culture medium and is compounded with soybean powder, corn flour, sucrose, yeast and vitamins. The ingredients of the compound are shown in Table 2.
The number of the planting of each group of the test group is 160 bottles, the number of the test group is 16, and the test period is 4 months.
TABLE 2
(2) The strains are all strains in the same batch.
The darkroom mycelium showed: under the dark condition, the mycelium propagation time of the test group is 5-7 days, and the colony infection coverage rate is 70-95%. Test group 7 performed best, the propagation time was 5 days, the hypha coverage reached 95%, the colony density was large, and the color was white.
(3) Results
(1) Color conversion performance: the test group is subjected to illumination cultivation, the color is changed from white to orange, the color is gradually deepened, the color changing time is 3-7 days, sorghum grains are changed into orange after 7 days, the mycelium has sexual reproduction stage and begins to enter sexual reproduction stage, the color is changed to fruit body development, and the number of the color changing of the infected sorghum grains is large. At this time, cordycepin, cordycepic acid, etc. which are main components are converted and produced, and the yield of the sub-entity is also determined. Among them, test group 7 performed optimally, and color change was completed in 3 days.
(2) Fruiting stage shows: fruiting body develops into fruiting body, and the number of fruiting body is about 10-60, and the fruiting body is pale yellow-orange. Wherein, the test group 7 shows best performance, fruiting starts on the 30 th day, and grows neatly until 35 days, and the number of fruiting bodies is 50-60, and the fruiting bodies grow rapidly.
(3) Maturity performance: the overall harvest and growth period of the test group is 45-60 days, and the height of the fruiting body is 3-10cm. In the production process of the test group 7, maldevelopment caused by insufficient nutrition does not occur, and cordyceps sinensis matures and ages in advance; the collected fruiting body is orange in color, at the initial stage of spore production, the growth height is 8-10cm, the collecting period is 60 days, and under the condition of refrigeration, the Cordyceps sinensis can be ensured to be in fresh state for more than 10 days.
And (3) comprehensive evaluation: the formulation proportion is feasible for the whole cordyceps militaris and has good performance. Has special nutritive value and obvious medicinal value. The Cordyceps sinensis beverage contains abundant proteins, 18 amino acids, 17 trace elements, 12 vitamins, and Cordyceps sinensis effective components such as cordycepin, cordycepic acid, cordyceps sinensis polysaccharide, etc. Wherein, the cordycepin content is more than 2 percent, compared with cordycepin in cordyceps flower less than 0.2 percent and cordycepin in cordyceps flower less than 0.1 percent, the effective components of adenosine cultivated by using the formula are obviously higher than other medicinal cordyceps. Of these, test group 7 performed best.
According to the formula of the cultivation matrix, in the cultivation process, fruiting bodies develop robustly, grow regularly and have better productivity; the growth process is stable, and the yield rate is more than 95%.
According to the embodiment of the invention, the culture medium for cordyceps militaris adopts sorghum as a matrix, and is compounded with soybean meal, corn meal, sucrose, yeast and vitamins, so that the cost is reduced, the requirement of good growth of cordyceps militaris is fully met, and the quality of cordyceps militaris is improved.
The foregoing description is only a preferred embodiment of the present invention, and is not intended to limit the embodiment of the present invention in any way, but any simple modification, equivalent variation and modification of the above embodiment according to the technical substance of the embodiment of the present invention still fall within the scope of the technical solution of the embodiment of the present invention.
Claims (5)
1. A culture medium for cordyceps militaris, which is characterized by comprising the following components: sorghum, soybean meal, corn meal, sucrose, yeast, and vitamins.
2. The cultivation substrate according to claim 1, wherein,
the vitamin is vitamin b1.
3. The cultivation substrate according to claim 1, wherein,
the cultivation substrate comprises the following raw materials in parts by weight: 0.5-2.0 parts of soybean powder, 0.1-1.6 parts of corn flour, 0-0.75 parts of sucrose, 0.05-0.07 parts of yeast, 0.002-0.004 parts of vitamin b1 and 27-33 parts of sorghum.
4. A culture substrate according to claim 3, wherein,
the cultivation substrate comprises the following raw materials in parts by weight: 0.5-2.0 parts of soybean powder, 0.1-1.6 parts of corn flour, 0-0.75 parts of sucrose, 0.061 parts of yeast, 0.0032 parts of vitamin b1 and 30 parts of sorghum.
5. The cultivation substrate according to claim 4, wherein,
the cultivation substrate comprises the following raw materials in parts by weight: 3.4 parts of soybean powder, 3.1 parts of corn flour, 0.9 part of sucrose, 0.19 part of yeast, 0.01 part of vitamin b1 and 92.4 parts of sorghum.
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| CN202311025886.0A CN116830967A (en) | 2023-08-15 | 2023-08-15 | Cordyceps militaris cultivation medium |
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| CN202311025886.0A CN116830967A (en) | 2023-08-15 | 2023-08-15 | Cordyceps militaris cultivation medium |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301912A (en) * | 2011-07-01 | 2012-01-04 | 西藏月王生物技术有限公司 | Method for culturing Cordyceps militaris by using highland barley as main raw material |
| CN102870600A (en) * | 2012-10-17 | 2013-01-16 | 山西万海澳生物科技有限责任公司 | Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content |
| JP2022008263A (en) * | 2020-06-25 | 2022-01-13 | テーブルマーク株式会社 | Mushroom cultivation medium additive agent |
| CN114600706A (en) * | 2022-03-14 | 2022-06-10 | 连云港市农业科学院 | Artificial culture method of cordyceps militaris |
| KR20230065626A (en) * | 2021-11-05 | 2023-05-12 | 이영애 | Artificial cultivation method of Cordyceps |
-
2023
- 2023-08-15 CN CN202311025886.0A patent/CN116830967A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301912A (en) * | 2011-07-01 | 2012-01-04 | 西藏月王生物技术有限公司 | Method for culturing Cordyceps militaris by using highland barley as main raw material |
| CN102870600A (en) * | 2012-10-17 | 2013-01-16 | 山西万海澳生物科技有限责任公司 | Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content |
| JP2022008263A (en) * | 2020-06-25 | 2022-01-13 | テーブルマーク株式会社 | Mushroom cultivation medium additive agent |
| KR20230065626A (en) * | 2021-11-05 | 2023-05-12 | 이영애 | Artificial cultivation method of Cordyceps |
| CN114600706A (en) * | 2022-03-14 | 2022-06-10 | 连云港市农业科学院 | Artificial culture method of cordyceps militaris |
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