CN116814770A - 血浆外泌体标志物作为动脉粥样硬化诊断标志物的应用 - Google Patents

血浆外泌体标志物作为动脉粥样硬化诊断标志物的应用 Download PDF

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CN116814770A
CN116814770A CN202310769433.2A CN202310769433A CN116814770A CN 116814770 A CN116814770 A CN 116814770A CN 202310769433 A CN202310769433 A CN 202310769433A CN 116814770 A CN116814770 A CN 116814770A
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张婉
崔佳森
李黎
符伟国
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Abstract

本发明涉及分子诊断技术领域(IPC分类号为C12Q1/6883)，尤其涉及血浆外泌体标志物作为动脉粥样硬化诊断标志物的应用，所述血浆外泌体标志物为环状hsa_circ_0001360。本发明首先基于exoRBase外泌体数据库构建了CHD患者血液外泌体中circRNA/lncRNA‑miRNA‑mRNA调控网络。通过机器学习算法筛选出CHD患者特异性的外泌体hsa＿circ＿0001360作为诊断标志物，并通过实时定量逆转录‑聚合酶链反应(RT‑qPCR)在AS患者中进行验证。

Description

血浆外泌体标志物作为动脉粥样硬化诊断标志物的应用
技术领域
本发明涉及分子诊断技术领域(IPC分类号为C12Q1/6883)，尤其涉及血浆外泌体标志物作为动脉粥样硬化诊断标志物的应用。
背景技术
动脉粥样硬化(atherosclerosis，AS)是一种全身性、多灶性疾病，是心血管疾病的主要病理基础，冠心病(coronary heart disease，CHD)是心血管疾病的常见临床表现，在全球范围内导致患者残疾和死亡，对患者的身心健康和社会医疗资源造成巨大负担。AS的发展受到多种危险因素影响并隐匿发生，常常在临床检出时已累及严重血管病变。因此，早期AS的识别有助于疾病的预防和治疗AS本身和AS相关的不良心血管事件，从而降低发病率和死亡率。
外泌体是一种由细胞分泌直径在30～100nm的膜结合胞外囊泡，其中包含来自相应细胞的生物分子，如非编码RNA、mRNA和蛋白质。非编码RNA是指不编码蛋白质的RNA，包括环状RNA(circRNAs)、长链非编码RNA(lncRNAs)、微RNA(miRNAs)和功能未知的RNA，在细胞增殖、分化、迁移等生物过程中发挥着重要作用。这些生物活性分子使外泌体作为有效的交流媒介发挥作用，可以传递遗传信息并参与生理和病理过程。大量研究证实了血液外泌体、AS和心血管疾病之间的联系。CircRNAs具有保守的环状结构和对RNase更强的抗性，通过竞争性的内源性调控机制，可以作为miRNA海绵，进而参与基因表达的调控。最近，来自血液外泌体的circRNA引起了越来越多的关注。基于AS和心血管疾病的高患病率，来自血液外泌体的circRNA可能在心血管疾病的诊断和治疗中发挥重要作用。最近的研究也确定了外泌体在炎症反应的细胞间通讯中起着至关重要的作用，这意味着外泌体和AS之间有着密切的关系。然而，参与AS发病机制的外泌体相关非编码RNA至今尚未完全阐明。
中国专利CN111455037A公开了基于血浆外泌体环状RNA的粥样动脉硬化分子诊断标志物及其应用，利用高通量测序和实时定量PCR实验证明，与健康人群相比，粥样动脉硬化患者血浆外泌体中环状RNA hsa_circ_0005540的表达水平显著升高，hsa_circ_0005540可以作为粥样动脉硬化的分子诊断标志物，开发更多准确度高、应用效果好的标志物具有重要意义。
发明内容
本发明的第一个方面提供了血浆外泌体标志物作为动脉粥样硬化诊断标志物的应用，所述血浆外泌体标志物为环状hsa_circ_0001360和/或环状hsa_circ_0001360的基因表达的产品。
本发明的第二个方面提供了检测环状hsa_circ_0001360的基因表达的产品在制备诊断粥样动脉硬化的工具中的应用。
作为一种优选的实施方式，所述检测环状hsa_circ_0001360的基因表达的产品包括检测环状hsa_circ_0001360表达水平的产品。
作为一种优选的实施方式，与健康人群相比，受试者样本中hsa_circ_0001360的表达水平升高，则诊断该受试者为动脉粥样硬化患者。
作为一种优选的实施方式，所述受试者样本的来源是血浆外泌体。
作为一种优选的实施方式，所述产品为能够结合hsa_circ_0001360的核酸，所述核酸包括特异性扩增hsa_circ_0001360的引物，所述引物的序列如SEQ ID NO：1和SEQ IDNO：2所示。
本发明的第三个方面提供了一种获取血浆外泌体标志物的方法，包括以下步骤：
S1.构建CircRNA/lncRNA-miRNA-mRNA调控网络；
S2.样本中血液外泌体circRNA的筛选；
S3.样本中血液外泌体circRNA的表达验证。
作为一种优选的实施方式，所述获取血浆外泌体标志物的方法还包括：获取数据及预处理、差异表达基因、circRNAs和lncRNAs的筛选、预测差异表达基因、circRNA和lncRNA的潜在miRNA。
进一步的，所述获取数据及预处理具体包括通过RNA测序数据分析检测后，可以通过ExoRBase数据库收集并整合健康个体和相应疾病患者的RNA表达谱。
ExoRBase是人类血液外泌体来源的长链RNA存储库，包括circRNAs，lncRNAs和mRNAs。
进一步的，所述差异表达基因、circRNAs和lncRNAs的筛选具体包括采用“sva”包去除批处理效应，通过“limma”包比较粥样动脉硬化组和对照组筛选出差异表达基因、circRNAs和lncRNAs。
进一步的，使用“clusterProfiler”包(版本“3.10.1”)中的“enrichGO”和“enrichKEGG”函数进行基因本体(GO)注释和京都基因与基因组百科(KEGG)进行功能和通路富集分析。
本发明从CHD患者的血液外泌体中鉴定出85种差异表达的circRNA，43种差异表达的lncRNA和312种差异表达的mRNA，其中差异基因主要富集在炎症免疫应答等生物过程，与AS的发病机制密切相关。另外，KEGG分析显示，差异基因主要参与“中性粒细胞胞外陷阱形成(NETs)”及“NOD样受体介导的信号通路”。
NETs是由解聚的染色体和中性粒细胞来源的核蛋白、细胞质蛋白和颗粒蛋白组成的大型网状结构，能够诱捕和杀死病原体。NET结构作为一个平台，提供高局部浓度的效应分子，消除入侵者。因此，NETs被认为是先天免疫对抗病原体的重要防御工具，但也被认为与癌症、自身免疫、慢性炎症的病理生理学有关。目前，在人类和小鼠的AS病变和动脉血栓中也发现了NETs网状结构。在功能上，NETs已被证明可诱导内皮细胞、抗原提呈细胞和血小板的激活，导致促炎免疫反应。这表明它们不仅存在于斑块和血栓中，而且还可能在触发AS形成和动脉血栓形成中发挥致病作用。对AS的深入分析显示，凋亡的内膜和平滑肌细胞丰富区域聚集有大量NETs，暗示NETs促进斑块的破坏。NETs对血栓形成、炎症和血管损伤的放大作用，突出了NETs形成和作为治疗靶点的潜力，其中DNase-1可以通过分解DNA链来溶解NETs结构。实验和初步临床研究表明，灌注DNase对缺血再灌注损伤后的心肌梗死有改善作用。
NOD样受体因其在先天性免疫应答中的关键作用受到广泛关注，其中核苷酸结合寡聚化结构域样受体蛋白3(NOD-like receptor protein 3，NLRP3)是炎症小体的关键调控蛋白之一，NLRP3的激活及其相关分子调控信号通路参与多种心血管事件的发生发展。由于NLRP3炎性小体是IL-1家族细胞因子的主要生产者，作为联系代谢紊乱和炎症反应的桥梁，因此它在动脉粥样硬化发病机制中的作用被深入研究。现有研究表明NLRP3被大量存在于动脉粥样硬化病变中的各种内源性危险信号激活，如氧化低密度脂蛋白和胆固醇晶体。NLRP3炎症小体的激活有助于血管炎症反应，推动动脉粥样硬化的发展和进展。
进一步的，所述预测差异表达基因、circRNA和lncRNA的潜在miRNA具体包括通过ENCORI预测差异表达mRNA和circRNA的潜在结合的miRNA，预测结果通过TargetScan和miRanda数据库进行进一步验证，选择miRNA预测结果交集进行后续分析。miRcode数据库提供了转录组范围内microRNA靶标预测，用于研究lncRNA-miRNA相互作用。
进一步的，利用“glmnet”包进行lasso回归模型变量选择，筛选AS相关的重要特征变量，进行最小绝对收缩和选择算子。
作为一种优选的实施方式，所述构建CircRNA/lncRNA-miRNA-mRNA调控网络的方法包括：首先通过ENCORI、TargetScan和miRanda数据库预测靶向差异mRNA的miRNA和靶向差异表达circRNA的miRNA，其次从miRcode数据库中获取包含差异表达lncRNA结合位点的miRNA，基于竞争性内源性调控网络选择重叠的miRNAs，最后利用“ggalluvial”包构建参与受试者样本的血液外泌体的CircRNA/lncRNA-miRNA-mRNA调控网络并进行可视化。
本发明的第四个方面提供了一种探针，所述探针能够检测受试者样本中hsa_circ_0001360的表达水平。
本发明的第五个方面提供了一种包括所述探针的试剂盒，所述试剂盒可以用于诊断动脉粥样硬化。
与现有技术相比，本发明具有以下有益效果：
1.本发明首先基于exoRBase外泌体数据库构建了CHD患者血液外泌体中circRNA/lncRNA-miRNA-mRNA调控网络。通过机器学习算法筛选出CHD患者特异性的外泌体hsa＿circ＿0001360作为诊断标志物，并通过实时定量逆转录-聚合酶链反应(RT-qPCR)在AS患者中进行验证。
2.本发明从CHD患者的血液外泌体中鉴定出85种差异表达的circRNA，43种差异表达的lncRNA和312种差异表达的mRNA。其中差异基因主要富集在炎症免疫应答等生物过程，与AS的发病机制密切相关。此外，KEGG分析显示，差异基因主要参与“中性粒细胞胞外陷阱形成(NETs)”及“NOD样受体介导的信号通路”。
3.本发明基于与AS相关的circRNA/lncRNA-miRNA-mRNA调控网络确定了15个circRNA，并通过lasso回归筛选特征变量hsa_circ_0001360，标志物hsa_circ_0001360在CHD患者的血液外泌体中被进一步验证。
4.本发明所构建的circRNA/lncRNA-miRNA-mRNA调控网络有助于了解AS的发病机制。
5.本发明的标志物hsa_circ_0001360可以快速、特异和灵敏地诊断粥样动脉硬化，还可以进一步制备成相关检测产品进行市场应用。
附图说明
图1为正常人和CHD患者血液外泌体中差异表达的mRNA、lncRNA和circRNA，其中A为差异表达的mRNA，B为差异表达的lncRNA，C为差异表达的circRNA。
图2为CircRNA/lncRNA-miRNA-mRNA调控网络，中间部分外圆为miRNA，内圆为mRNA，连线代表预测存在相互作用。
图3为ExoRBase数据库CHD患者血液外泌体circRNA的筛选，其中A为15个circRNA的LASSO回归系数路径图，曲线3表示hsa_circ_0001360，B为LASSO回归的交叉验证曲线，C为ROC曲线评估hsa_circ_0001360的诊断价值。
图4为血液外泌体circRNA抽提与差异表达验证，其中A为CHD患者血浆外泌体形态特征，B为纳米颗粒跟踪分析CHD患者血浆外泌体大小分布，C为RT-qPCR检测对照组和CHD组hsa_circ_0001360的mRNA表达差异，D为ROC曲线评估hsa_circ_0001360的诊断价值。
具体实施方式
实施例
受试者选择：选择10例于2021年8月至2021年12月在复旦大学附属华东医院纳入的CHD患者作为实验组，患者诊断基于病史，体格检查和影像学检查，和10例健康(无心血管疾病、糖尿病或高脂血症)个体作为对照组，实验组和对照组统称为受试者。
本实施例的探究实验由复旦大学附属华东医院伦理委员会(2017K001)批准。所有患者在加入研究前都提供了书面知情同意书。排除标准包括：(1).肿瘤或结缔组织疾病史，(2).3个月内使用类固醇激素类药物，(3).近期感染，(4)未签署知情同意书。
血清外泌体获取：受试者禁食8小时后，于上午8：00采集血液样本。新鲜血液样本在4℃下以3000g的速度离心10分钟，将上清转移到另一个试管中，使用HieffTMQuick外泌体分离试剂盒(购买自翌圣生物技术有限公司)分离血清外泌体，通过透射电镜和纳米颗粒跟踪分析进一步评估血清来源的外泌体的形态特征和大小分布(图4A和B)。
总RNA获取：采用TRIzol试剂从血清外泌体中提取总RNA。随后使用IIIcDNA合成试剂盒(购买自翌圣生物技术有限公司)进行circRNA的反转录。基于ABIQuantStudio 5Real-Time PCR系统(赛默飞世尔科技有限公司)进行RT-qPCR，比较目标circRNA的表达水平。相应引物通过circPrimer 1.2设计。
统计分析采用独立t检验(SPSS 24.0版本)进行。P值小于0.05被认为是具有显著差异。
获取数据及预处理：
通过规范化RNA测序数据分析检测后，ExoRBase收集并整合了健康个体和相应疾病患者的RNA表达谱。本研究下载正常人(n＝32)和粥样动脉硬化患者(n＝6)的RNA表达谱，通过R软件(4.0.2版本)进行背景校正。
差异表达基因、circRNAs和lncRNAs的筛选：
上述32例正常和6例CHD患者样本的数据用于筛选差异表达的circRNA，lncRNA和mRNA。基于P值小于0.05的截断标准，鉴定出85个差异表达的circRNA(4个上调，81个下调)，43个差异表达的lncRNA(24个上调，19个下调)和312个差异表达的基因(55个上调，257个下调，如图1A所示)。GO分析揭示差异mRNA主要富集在炎症免疫应答等生物过程，KEGG分析差异基因主要与中性粒细胞胞外陷阱形成和核苷酸结合寡聚化结构域(nucleotide-bindingoligomerization domain，NOD)样受体介导的信号通路相关(图1B和C)。
circRNA/lncRNA-miRNA-mRNA网络的构建：
通过ENCORI、TargetScan和miRanda数据库预测了176个靶向差异mRNA的miRNA和171个靶向差异表达circRNA的miRNA接下来从miRcode数据库中获得166个包含差异表达lncRNA结合位点的miRNA。基于竞争性内源性调控网络选择重叠的miRNAs，最后，利用“ggalluvial”包构建了参与CHD患者的血液外泌体的circRNA/lncRNA-miRNA-mRNA调控网络并进行可视化(图2A，B)。
进一步的，通过Lasso回归筛选出hsa_circ_0001360作为AS的诊断标志物(图3A，B)。进一步进行ROC曲线分析，评估hsa_circ_0001360在AS中的潜在诊断价值(图3C)，hsa_circ_0001360曲线下面积为0.844，95％置信区间为0.705-0.982。
对受试者的血清外泌体进行形态特征和大小分布分析后，采用RT-qPCR试验进行后续验证hsa_circ_0001360的表达，如图2C所示，hsa_circ_0001360在CHD患者中的相对表达量显著上调(P<0.05，图4C)。进一步进行ROC曲线分析，评估hsa_circ_0001360在AS中的潜在诊断价值(图4D)。hsa_circ_0001360曲线下面积为0.880，95％置信区间为0.7057-1.000。
5个差异表达的circRNA，4个上调，81个下调，详情如下：
"symbol","change"
"hsa_circ_0075796","down"
"hsa_circ_0000982","down"
"hsa_circ_0001072","down"
"hsa_circ_0000842","down"
"hsa_circ_0031446","down"
"hsa_circ_0000019","down"
"hsa_circ_0000038","down"
"hsa_circ_0000160","down"
"hsa_circ_0006760","down"
"hsa_circ_0008817","down"
"hsa_circ_0007587","down"
"hsa_circ_0001603","down"
"hsa_circ_0000997","down"
"hsa_circ_0073371","down"
"hsa_circ_0001016","down"
"hsa_circ_0000722","up"
"hsa_circ_0006062","down"
"hsa_circ_0001684","down"
"hsa_circ_0003914","down"
"hsa_circ_0013093","down"
"hsa_circ_0001439","up"
"hsa_circ_0004751","down"
"hsa_circ_0001680","down"
"hsa_circ_0000311","down"
"hsa_circ_0077083","down"
"hsa_circ_0008153","down"
"hsa_circ_0001163","down"
"hsa_circ_0006856","down"
"hsa_circ_0004058","down""hsa_circ_0007619","down""hsa_circ_0001939","down""hsa_circ_0005912","down""hsa_circ_0000072","down""hsa_circ_0006987","down""hsa_circ_0002158","down""hsa_circ_0069492","down""hsa_circ_0001360","up""hsa_circ_0001280","down""hsa_circ_0008857","down""hsa_circ_0002199","down""hsa_circ_0001309","down""hsa_circ_0008201","down""hsa_circ_0004813","down""hsa_circ_0005871","down""hsa_circ_0013252","down""hsa_circ_0004622","down""hsa_circ_0007370","down""hsa_circ_0062938","down""hsa_circ_0007664","down""hsa_circ_0075675","down""hsa_circ_0002551","down""hsa_circ_0001540","down""hsa_circ_0000091","down""hsa_circ_0067619","down""hsa_circ_0008901","down""hsa_circ_0045462","down""hsa_circ_0002972","down""hsa_circ_0047920","down""hsa_circ_0002094","down""hsa_circ_0005232","down""hsa_circ_0006354","down""hsa_circ_0004592","down""hsa_circ_0008463","down"
"hsa_circ_0008488","down"
"hsa_circ_0007974","down"
"hsa_circ_0003357","down"
"hsa_circ_0004831","down"
"hsa_circ_0066874","down"
"hsa_circ_0001818","down"
"hsa_circ_0000043","down"
"hsa_circ_0001648","down"
"hsa_circ_0081819","down"
"hsa_circ_0000639","down"
"hsa_circ_0005227","down"
"hsa_circ_0007026","down"
"hsa_circ_0008982","down"
"hsa_circ_0000896","up"
"hsa_circ_0005965","down"
"hsa_circ_0025843","down"
"hsa_circ_0076881","down"
"hsa_circ_0008934","down"
"hsa_circ_0004791","down"
"hsa_circ_0001355","down"
"hsa_circ_0001020","down"
"hsa_circ_0002371","down"
43个差异表达的lncRNA24个上调，19个下调，详情如下："symbol","change"
"RPL21P119","up"
"FTH1P20","up"
"RP11-186B7.4","down"
"RPL21P75","up"
"TPM3P8","up"
"RP11-793H13.8","down"
"H3F3AP6","up"
"EEF1A1P9","up"
"HNRNPA1P35","up"
"HMGN2P41","up"
"CTB-89H12.4","down""RPS2P7","up"
"RP11-493E12.3","down""NUDT4P2","up"
"RPL7AP66","up"
"RP11-120M18.2","down""CTD-2562J17.6","down""SLX1A-SULT1A3","up""RP11-227G15.3","down""PRR13P5","up"
"RP11-156P1.3","down""RPL7AP11","up"
"CTD-2336O2.1","down""AC093642.5","down"
"RPL9P7","up"
"ZNF322P1","up"
"RP11-673C5.1","down""H3F3BP1","up"
"EEF1A1P11","up"
"RP11-181C21.4","down""RP11-29G8.3","down""SLX1B-SULT1A4","up""RASA4DP","up"
"PKD1P1","up"
"RP11-43N16.4","down""GTF2IP4","down"
"LINC00674","up"
"AC007318.5","down"
"RP11-21J18.1","down""HSPA8P8","up"
"RPL13AP7","up"
"RP11-206F17.2","down""RP11-218M22.1","down"
312个差异表达的基因，55个上调，257个下调，详情如下："symbol","change"
"ERBIN","down"
"RPL21","up"
"SLF1","down"
"RPL36A","up"
"RPL36A-HNRNPH2","up"
"ARHGAP45","down"
"HIST1H3D","up"
"GRK3","down"
"NRDC","down"
"EMSY","down"
"AC005943.2","down"
"SLC25A6","up"
"AF011889.5","down"
"AL109923.1","down"
"USF3","down"
"RGPD6","up"
"RP11-474G23.1","down"
"CTD-3214H19.4","down"
"PPP4R3B","down"
"SRPRA","down"
"CTD-2545G14.7","down"
"RP11-231C18.3","down"
"SLF2","down"
"RP11-111K18.1","down"
"TMX2-CTNND1","up"
"KMT5B","down"
"CTD-2132N18.3","down"
"WAPL","down"
"AL078584.1","down"
"RP11-286N22.8","down"
"HSPA1B","up"
"TCEAL9","down"
"RP11-302B13.5","down"
"RP11-146B14.1","down"
"RP11-295K3.1","down"
"PTPA","down"
"AL513412.1","down"
"CAMP","up"
"SPDYA","down"
"UTP4","down"
"CARNMT1","down"
"AC073610.5","down"
"THAP12","down"
"PPP4R3A","down"
"RP11-561B11.2","down"
"SNU13","down"
"RP11-2C24.9","down"
"SVBP","down"
"NCBP3","down"
"VPS50","down"
"RP5-1028K7.3","down"
"RP11-512M8.5","down"
"RP11-507K12.1","down"
"SMIM10L1","down"
"RP11-542C16.2","down"
"GRK2","down"
"RP11-73M18.2","down"
"RP11-426L16.10","down"
"DEFA1B","up"
"RP11-468E2.4","down"
"LINS1","down"
"RGS3","up"
"RGPD5","up"
"RP11-529K1.3","down"
"XXbac-BPG32J3.22","down""RP11-449H3.3","down""NSMCE3","down"
"RP11-437B10.1","down""AL356053.1","down"
"RP4-583P15.15","down""HIST2H2AA4","up"
"RP11-123K3.4","down""AC013264.1","down"
"HIST1H4K","up"
"TOMM70","down"
"DEFA3","up"
"EFL1","down"
"NUP58","down"
"U2AF1","up"
"MPO","up"
"DEFA1","up"
"ABHD18","down"
"AC007192.4","down"
"AZU1","up"
"AC068533.7","down"
"MMP8","up"
"PCNX1","down"
"RP11-307N16.6","down""CTC-432M15.3","down""HIST2H4B","up"
"RPL39","up"
"HIST2H4A","up"
"TRIM69","down"
"CTD-2207O23.3","down""RP11-762I7.5","down""CTD-2370N5.3","down""RP11-468E2.1","down""RP5-1021I20.4","down""RP11-49K24.6","down""AC127070.1","down"
"RUBCN","down"
"AL928654.7","down"
"RP11-574F21.3","down""RP4-635E18.9","down""DCAF1","down"
"KMT5A","down"
"GCN1","down"
"RP11-864I4.1","down"
"SHTN1","down"
"ARPC4-TTLL3","down""FAAP20","down"
"RACK1","down"
"AP000275.65","down"
"CTD-2116N17.1","down""PUM3","down"
"RP11-1035H13.3","down""RP11-849F2.7","down""AP000304.12","down"
"CTB-50L17.10","down""AC009133.23","down"
"RP11-244H3.4","down""CTC-435M10.3","down""RP11-382A20.3","down""AL513122.2","down"
"AL513122.1","down"
"AC104534.3","down"
"RP11-507M3.1","down""CTD-2192J16.20","down""RP11-644F5.10","down""H3F3A","up"
"CNOT9","down"
"HIST2H3C","up"
"MARC1","up"
"AC096949.1","down"
"RP11-77K12.1","down""RP5-864K19.6","down""LCOR","down"
"HIST1H2AM","up"
"CTC-260F20.3","down""AC009950.1","down"
"RP11-407N17.3","down""PRKCB","down"
"HIST1H3G","up"
"MFSD14A","down"
"PRELID3B","down"
"LLfos-48D6.2","down""RP11-111H13.1","down""RP11-96O20.4","down""CTSG","up"
"RP11-314N13.10","down""AC090498.1","down"
"TMEM94","down"
"MARC2","up"
"RP5-850E9.3","down"
"CTD-2278I10.6","down""RP1-34B20.21","down""CTD-3138B18.4","down""FCMR","down"
"AL358113.1","down"
"RP11-12J10.3","down"
"AC013461.1","down"
"CTD-2369P2.12","down""RP11-152F13.10","down""HIST2H2AA3","up"
"HIST1H4L","up"
"CTC-487M23.8","down""RP11-463D19.2","down""RP11-697E2.6","down""AC010642.1","down"
"RP11-176H8.1","down""RP11-343C2.11","down""AC027682.1","down"
"RP4-559A3.7","down"
"INTS6L","down"
"RP11-552F3.12","down""RP11-411B6.6","down""PRICKLE4","down"
"RP11-438J1.1","down""BOLA2","up"
"EIF3CL","up"
"RP11-598P20.5","down""RP11-701H24.9","down""BORCS6","down"
"RP11-212D19.4","down""CTB-133G6.1","down""RP11-295P9.13","down""CTD-2410N18.5","down""NPR2","up"
"AC026348.1","down"
"ERVK3-1","down"
"CRAMP1","down"
"AL022067.1","down"
"RP11-33O4.2","down"
"KAT14","down"
"SNX6","down"
"ZBED1","up"
"PCMTD1","down"
"BBOF1","down"
"HIST1H3B","up"
"JAML","down"
"HIST1H2AI","up"
"XXbac-BPG32J3.20","down""CTD-2192J16.22","down""IFI30","up"
"CALM1","down"
"QTRT2","down"
"PRUNE1","down"
"PCNX3","down"
"RP11-793H13.10","down""FBXO16","down"
"AC023283.1","down"
"RP11-574K11.31","down""ANKRD12","down"
"RP11-330H6.5","down"
"GPAT4","down"
"NBPF10","up"
"UBC","up"
"RCC1L","down"
"AC069368.3","down"
"SGF29","down"
"AL034550.1","down"
"NDUFV2","up"
"COPS9","down"
"UTP11","down"
"JCHAIN","down"
"AL021546.6","down"
"KYAT3","down"
"RP11-691N7.6","down"
"PYM1","down"
"RGPD8","up"
"HIST1H4J","up"
"RP13-279N23.2","down"
"TAOK3","down"
"PAGR1","up"
"CTC-479C5.12","down"
"RP11-156P1.2","down"
"ZEB2","down"
"AC113404.1","down"
"AC024592.12","down"
"XXbac-BPG181M17.5","down""RP11-51F16.8","down"
"HIST1H3C","up"
"RP11-603J24.9","down"
"CARMIL2","down"
"RP11-613M10.9","down"
"RP11-745O10.4","down"
"AC090094.1","down"
"CTD-2550O8.5","down"
"RP11-315D16.2","down"
"RP11-729L2.2","down"
"KIF1BP","down"
"RP1-37E16.12","down"
"MRM2","down"
"AC010547.9","down"
"CTD-2021K4.1","down"
"CTD-2545M3.6","down"
"BANK1","down"
"U2AF1L5","down"
"TKFC","down"
"NEPRO","down"
"ARID2","down"
"RP11-618P17.4","down"
"FAM234A","down"
"MCRIP1","down"
"LUZP6","down"
"CALM2","down"
"MORC3","down"
"NAXE","down"
"RP11-458D21.5","down"
"AP003419.11","down"
"RP11-108K14.8","down"
"HIST1H1B","up"
"RP11-20I23.3","down"
"MAN1A2","down"
"RP5-972B16.2","down"
"HIST1H2AJ","up"
"PCNX4","down"
"S100A6","up"
"SLX1B","up"
"RP11-140L24.4","down"
"VPS41","down"
"FAM209A","down"
"MT-CO1","down"
"AC002985.3","down"
"AC037459.4","down"
"TBC1D26","down"
"SLX1A","up"
"LA16c-431H6.6","down"
"BORCS7","down"
"CH17-270A2.2","down"
"HBA1","up"
"XXbac-BPG246D15.9","down""TIGAR","down"
"CTD-3222D19.2","down"
"RP11-468E2.2","down"
"HPF1","down"
"AL135791.1","down"
"BEST1","down"
"SYCP2","down"
"RP3-468K18.7","down"
"XPO1","down"
"MFSD14B","down"
"HNRNPA3","up"
"VAMP7","up"
"SEP15","down"
"TRMO","down"
"PRTN3","up"
"NUDT3","down"
"EEF1AKMT1","down"
"RP11-831H9.11","down"
"AP000295.9","down"
"PEX26","down"
176个靶向差异mRNA的miRNA，详情如下：mRNA miRNA SLF2 hsa-let-7a-5p
INTS6L hsa-let-7a-5p
SLF2 hsa-let-7b-5p
INTS6L hsa-let-7b-5p
SLF2 hsa-let-7c-5p
INTS6L hsa-let-7c-5p
SLF2 hsa-let-7d-5p
INTS6L hsa-let-7d-5p
SLF2 hsa-let-7e-5p
INTS6L hsa-let-7e-5p
SLF2 hsa-let-7f-5p
INTS6L hsa-let-7f-5p
SLF2 hsa-let-7g-5p
INTS6L hsa-let-7g-5p
SLF2 hsa-let-7i-5p
INTS6L hsa-let-7i-5p
MFSD14A hsa-miR-1-3p
THAP12 hsa-miR-1-3p
ARID2 hsa-miR-1-3p
CALM2 hsa-miR-1-3p
HNRNPA3 hsa-miR-1-3p
GRK3 hsa-miR-1-3p
LCOR hsa-miR-101-3pEMSY hsa-miR-101-3pPRELID3B hsa-miR-101-3pERBINhsa-miR-101-3pLCOR hsa-miR-103a-3pPPP4R3B hsa-miR-103a-3pMFSD14B hsa-miR-103a-3pMFSD14A hsa-miR-106a-5pKMT5B hsa-miR-106a-5pEMSY hsa-miR-106a-5pTAOK3hsa-miR-106a-5pUBC hsa-miR-106a-5pANKRD12 hsa-miR-106a-5pPCMTD1 hsa-miR-106a-5pMFSD14A hsa-miR-106b-5pKMT5B hsa-miR-106b-5pEMSY hsa-miR-106b-5pTAOK3 hsa-miR-106b-5pUBC hsa-miR-106b-5pANKRD12 hsa-miR-106b-5pPCMTD1 hsa-miR-106b-5pLCOR hsa-miR-107PPP4R3B hsa-miR-107MFSD14B hsa-miR-107LCOR hsa-miR-124-3pSNX6 hsa-miR-124-3pCARNMT1 hsa-miR-124-3pAL358113.1 hsa-miR-124-3pSHTN1hsa-miR-125a-5pPPP4R3A hsa-miR-125a-5pSHTN1 hsa-miR-125b-5pPPP4R3A hsa-miR-125b-5pKMT5A hsa-miR-127-3p
SLF2 hsa-miR-1271-5pPPP4R3A hsa-miR-1271-5pARID2 hsa-miR-128-3pRGPD6hsa-miR-128-3pCALM1 hsa-miR-129-5pZEB2 hsa-miR-129-5pHNRNPA3 hsa-miR-129-5pMFSD14A hsa-miR-1297MAN1A2 hsa-miR-1297WAPL hsa-miR-1297SHTN1 hsa-miR-1297LCOR hsa-miR-1297ARID2 hsa-miR-1297KMT5A hsa-miR-1297PCNX1 hsa-miR-1297SLF2 hsa-miR-130a-3p
UBC hsa-miR-130a-3pFAM234A hsa-miR-130a-3pANKRD12 hsa-miR-130a-3pCALM2 hsa-miR-130a-3pZEB2 hsa-miR-130a-3pERBIN hsa-miR-130a-3p
SLF2 hsa-miR-130b-3p
UBC hsa-miR-130b-3pFAM234A hsa-miR-130b-3pANKRD12 hsa-miR-130b-3pCALM2 hsa-miR-130b-3pZEB2 hsa-miR-130b-3pERBIN hsa-miR-130b-3pARID2 hsa-miR-132-3pNUP58 hsa-miR-132-3pCALM1 hsa-miR-133a-3pXPO1 hsa-miR-133a-3pPRELID3B hsa-miR-133a-3pCALM1 hsa-miR-133bXPO1 hsa-miR-133bPRELID3B hsa-miR-133bWAPL hsa-miR-135b-5pLCOR hsa-miR-136-5pLCOR hsa-miR-137EMSY hsa-miR-137PPP4R3A hsa-miR-137ANKRD12 hsa-miR-137HNRNPA3 hsa-miR-137ERBIN hsa-miR-137LCOR hsa-miR-139-5pKMT5A hsa-miR-139-5pZEB2 hsa-miR-139-5pMFSD14A hsa-miR-140-5pERBIN hsa-miR-140-5pMFSD14A hsa-miR-141-3pEMSY hsa-miR-141-3pPCNX4 hsa-miR-141-3pZEB2 hsa-miR-141-3pPRELID3B hsa-miR-141-3pLCOR hsa-miR-142-3p
XPO1 hsa-miR-142-3pZEB2 hsa-miR-142-3pEMSY hsa-miR-144-3pARID2 hsa-miR-144-3pZEB2 hsa-miR-144-3pMORC3 hsa-miR-144-3pRGS3 hsa-miR-145-5p
SLF2 hsa-miR-148a-3pFAM234A hsa-miR-148a-3pTOMM70 hsa-miR-148a-3pRUBCN hsa-miR-148a-3pMFSD14B hsa-miR-148a-3pSLF2 hsa-miR-148b-3p
FAM234A hsa-miR-148b-3p
TOMM70 hsa-miR-148b-3p
RUBCN hsa-miR-148b-3p
MFSD14B hsa-miR-148b-3p
PTPA hsa-miR-150-5p
SLF2 hsa-miR-152-3p
FAM234A hsa-miR-152-3p
TOMM70 hsa-miR-152-3p
RUBCN hsa-miR-152-3p
MFSD14B hsa-miR-152-3p
MORC3 hsa-miR-153-3p
PPP4R3A hsa-miR-154-5p
H3F3A hsa-miR-155-5p
LCOR hsa-miR-155-5p
EMSY hsa-miR-155-5p
ARID2 hsa-miR-155-5p
HNRNPA3 hsa-miR-155-5p
MORC3 hsa-miR-155-5p
WAPL hsa-miR-15a-5p
SRPRA hsa-miR-15a-5p
CALM1 hsa-miR-15a-5p
RGPD6 hsa-miR-15a-5p
VPS50 hsa-miR-15a-5p
WAPL hsa-miR-15b-5p
SRPRA hsa-miR-15b-5p
CALM1 hsa-miR-15b-5p
RGPD6 hsa-miR-15b-5p
VPS50 hsa-miR-15b-5p
WAPL hsa-miR-16-5p
SRPRA hsa-miR-16-5p
CALM1 hsa-miR-16-5p
RGPD6 hsa-miR-16-5p
VPS50 hsa-miR-16-5p
MFSD14A hsa-miR-17-5p
KMT5B hsa-miR-17-5p
EMSY hsa-miR-17-5p
TAOK3 hsa-miR-17-5p
UBC hsa-miR-17-5p
ANKRD12 hsa-miR-17-5p
PCMTD1 hsa-miR-17-5p
LCOR hsa-miR-181a-5p
SLF2 hsa-miR-181a-5p
GRK2 hsa-miR-181a-5p
EMSY hsa-miR-181a-5p
ARID2 hsa-miR-181a-5p
CALM1 hsa-miR-181a-5p
TMEM94 hsa-miR-181a-5p
LCOR hsa-miR-181b-5p
SLF2 hsa-miR-181b-5p
GRK2 hsa-miR-181b-5p
EMSY hsa-miR-181b-5p
ARID2 hsa-miR-181b-5p
CALM1 hsa-miR-181b-5p
TMEM94 hsa-miR-181b-5p
LCOR hsa-miR-181c-5p
SLF2 hsa-miR-181c-5p
GRK2 hsa-miR-181c-5p
EMSY hsa-miR-181c-5p
ARID2 hsa-miR-181c-5p
CALM1 hsa-miR-181c-5p
TMEM94 hsa-miR-181c-5p
LCOR hsa-miR-181d-5p
SLF2 hsa-miR-181d-5p
GRK2 hsa-miR-181d-5p
EMSY hsa-miR-181d-5p
ARID2 hsa-miR-181d-5p
CALM1 hsa-miR-181d-5p
TMEM94 hsa-miR-181d-5p
ARID2 hsa-miR-182-5p
PCNX1 hsa-miR-182-5p
MORC3 hsa-miR-182-5p
TOMM70 hsa-miR-183-5p
SLF2 hsa-miR-186-5p
KMT5A hsa-miR-186-5p
NCBP3 hsa-miR-186-5p
RGPD6 hsa-miR-186-5p
TAOK3 hsa-miR-190a-5p
XPO1 hsa-miR-190a-5p
MFSD14B hsa-miR-190a-5p
TAOK3 hsa-miR-190b
XPO1 hsa-miR-190b
MFSD14B hsa-miR-190b
CALM1 hsa-miR-193a-3p
ERBIN hsa-miR-193a-3p
CALM1 hsa-miR-193b-3p
ERBIN hsa-miR-193b-3p
ARID2 hsa-miR-194-5p
KMT5A hsa-miR-194-5p
WAPL hsa-miR-195-5p
SRPRA hsa-miR-195-5p
CALM1 hsa-miR-195-5p
RGPD6 hsa-miR-195-5p
VPS50 hsa-miR-195-5p
LCOR hsa-miR-196a-5p
SLF2 hsa-miR-196a-5p
CALM1 hsa-miR-196a-5p
LCOR hsa-miR-196b-5p
SLF2 hsa-miR-196b-5p
CALM1 hsa-miR-196b-5p
SLF2 hsa-miR-199a-5p
FAM234A hsa-miR-199a-5p
SLF2 hsa-miR-199b-5pFAM234A hsa-miR-199b-5pSHTN1 hsa-miR-19a-3pEMSYhsa-miR-19a-3pCALM1 hsa-miR-19a-3pFAM234A hsa-miR-19a-3pANKRD12 hsa-miR-19a-3pZEB2 hsa-miR-19a-3pPTPA hsa-miR-19a-3pSHTN1 hsa-miR-19b-3pEMSY hsa-miR-19b-3pCALM1 hsa-miR-19b-3pFAM234A hsa-miR-19b-3pANKRD12 hsa-miR-19b-3pZEB2 hsa-miR-19b-3pPTPA hsa-miR-19b-3pMFSD14A hsa-miR-200a-3pEMSY hsa-miR-200a-3pPCNX4hsa-miR-200a-3pZEB2 hsa-miR-200a-3pPRELID3B hsa-miR-200a-3pWAPL hsa-miR-200b-3p
SLF2 hsa-miR-200b-3p
ZEB2 hsa-miR-200b-3pUSF3 hsa-miR-200b-3pWAPL hsa-miR-200c-3p
SLF2 hsa-miR-200c-3p
ZEB2 hsa-miR-200c-3pUSF3 hsa-miR-200c-3pANKRD12 hsa-miR-204-5pLCORhsa-miR-205-5pPCNX1 hsa-miR-205-5pZEB2 hsa-miR-205-5pMFSD14B hsa-miR-205-5pMFSD14A hsa-miR-206THAP12 hsa-miR-206ARID2 hsa-miR-206CALM2 hsa-miR-206HNRNPA3 hsa-miR-206GRK3 hsa-miR-206MFSD14A hsa-miR-20a-5pKMT5B hsa-miR-20a-5pEMSY hsa-miR-20a-5pTAOK3 hsa-miR-20a-5pUBC hsa-miR-20a-5pANKRD12 hsa-miR-20a-5pPCMTD1 hsa-miR-20a-5pMFSD14A hsa-miR-20b-5pKMT5B hsa-miR-20b-5pEMSYhsa-miR-20b-5pTAOK3 hsa-miR-20b-5pUBC hsa-miR-20b-5pANKRD12 hsa-miR-20b-5pPCMTD1 hsa-miR-20b-5pLCOR hsa-miR-21-5pANKRD12 hsa-miR-211-5pARID2 hsa-miR-212-3pNUP58 hsa-miR-212-3pPCNX1 hsa-miR-216a-5pMORC3 hsa-miR-216a-5pPPP4R3Ahsa-miR-216b-5pVPS41 hsa-miR-216b-5pWAPL hsa-miR-217ARID2 hsa-miR-217KMT5Ahsa-miR-217HNRNPA3 hsa-miR-217TAOK3 hsa-miR-218-5pZEB2 hsa-miR-218-5pHNRNPA3hsa-miR-218-5p
PRUNE1 hsa-miR-221-3p
SHTN1 hsa-miR-221-3p
HNRNPA3 hsa-miR-221-3p
PCMTD1 hsa-miR-221-3p
PRUNE1 hsa-miR-222-3p
SHTN1 hsa-miR-222-3p
HNRNPA3 hsa-miR-222-3p
PCMTD1 hsa-miR-222-3p
EMSY hsa-miR-223-3p
FBXO16 hsa-miR-223-3p
MFSD14A hsa-miR-23a-3p
GRK2 hsa-miR-23a-3p
EMSY hsa-miR-23a-3p
KMT5A hsa-miR-23a-3p
NCBP3 hsa-miR-23a-3p
PPP4R3B hsa-miR-23a-3p
ERBIN hsa-miR-23a-3p
VPS50 hsa-miR-23a-3p
MFSD14A hsa-miR-23b-3p
GRK2 hsa-miR-23b-3p
EMSY hsa-miR-23b-3p
KMT5A hsa-miR-23b-3p
NCBP3 hsa-miR-23b-3p
PPP4R3B hsa-miR-23b-3p
ERBIN hsa-miR-23b-3p
VPS50 hsa-miR-23b-3p
ARID2 hsa-miR-24-3p
USF3 hsa-miR-24-3p
RUBCN hsa-miR-24-3p
KIF1BP hsa-miR-25-3p
LCOR hsa-miR-25-3p
KMT5B hsa-miR-25-3p
SRPRA hsa-miR-25-3p
ZEB2 hsa-miR-25-3p
MORC3 hsa-miR-25-3p
ERBIN hsa-miR-25-3p
PCMTD1 hsa-miR-25-3p
RGS3 hsa-miR-25-3p
MFSD14A hsa-miR-26a-5p
MAN1A2 hsa-miR-26a-5p
WAPL hsa-miR-26a-5p
SHTN1 hsa-miR-26a-5p
LCOR hsa-miR-26a-5p
ARID2 hsa-miR-26a-5p
KMT5A hsa-miR-26a-5p
PCNX1 hsa-miR-26a-5p
MFSD14A hsa-miR-26b-5p
MAN1A2 hsa-miR-26b-5p
WAPL hsa-miR-26b-5p
SHTN1 hsa-miR-26b-5p
LCOR hsa-miR-26b-5p
ARID2 hsa-miR-26b-5p
PCNX1 hsa-miR-26b-5p
LCOR hsa-miR-27a-3p
KMT5B hsa-miR-27a-3p
ARID2 hsa-miR-27a-3p
PCNX1 hsa-miR-27a-3p
XPO1 hsa-miR-27a-3p
RGPD6 hsa-miR-27a-3p
RGPD5 hsa-miR-27a-3p
USF3 hsa-miR-27a-3p
LCOR hsa-miR-27b-3p
KMT5B hsa-miR-27b-3p
ARID2 hsa-miR-27b-3p
PCNX1 hsa-miR-27b-3p
XPO1 hsa-miR-27b-3p
RGPD6 hsa-miR-27b-3p
RGPD5 hsa-miR-27b-3p
USF3 hsa-miR-27b-3pKMT5B hsa-miR-29a-3pEMSY hsa-miR-29a-3p
IFI30 hsa-miR-29a-3pPPP4R3B hsa-miR-29a-3pUSF3 hsa-miR-29a-3pKMT5Bhsa-miR-29b-3pEMSY hsa-miR-29b-3p
IFI30 hsa-miR-29b-3pPPP4R3B hsa-miR-29b-3pUSF3 hsa-miR-29b-3pKMT5Bhsa-miR-29c-3pEMSY hsa-miR-29c-3p
IFI30 hsa-miR-29c-3pPPP4R3B hsa-miR-29c-3pUSF3 hsa-miR-29c-3pWAPLhsa-miR-300LCOR hsa-miR-300KMT5B hsa-miR-300EMSY hsa-miR-300PPP4R3B hsa-miR-300MFSD14B hsa-miR-300SLF2 hsa-miR-301a-3p
UBC hsa-miR-301a-3pFAM234A hsa-miR-301a-3pANKRD12 hsa-miR-301a-3pCALM2 hsa-miR-301a-3pZEB2 hsa-miR-301a-3pERBIN hsa-miR-301a-3p
SLF2 hsa-miR-301b-3p
UBC hsa-miR-301b-3pFAM234A hsa-miR-301b-3pANKRD12 hsa-miR-301b-3pCALM2 hsa-miR-301b-3p
ZEB2 hsa-miR-301b-3p
ERBIN hsa-miR-301b-3p
LCOR hsa-miR-302a-3p
KMT5B hsa-miR-302a-3p
EMSY hsa-miR-302a-3p
RUBCN hsa-miR-302a-3p
LCOR hsa-miR-302b-3p
KMT5B hsa-miR-302b-3p
EMSY hsa-miR-302b-3p
RUBCN hsa-miR-302b-3p
LCOR hsa-miR-302c-3p
KMT5B hsa-miR-302c-3p
EMSY hsa-miR-302c-3p
RUBCN hsa-miR-302c-3p
LCOR hsa-miR-302d-3p
KMT5B hsa-miR-302d-3p
EMSY hsa-miR-302d-3p
RUBCN hsa-miR-302d-3p
LCOR hsa-miR-302e
KMT5B hsa-miR-302e
EMSY hsa-miR-302e
RUBCN hsa-miR-302e
MFSD14A hsa-miR-30a-5p
MAN1A2 hsa-miR-30a-5p
SLF2 hsa-miR-30a-5p
THAP12 hsa-miR-30a-5p
XPO1 hsa-miR-30a-5p
ZEB2 hsa-miR-30a-5p
HNRNPA3 hsa-miR-30a-5p
MAN1A2 hsa-miR-30b-5p
SLF2 hsa-miR-30b-5p
THAP12 hsa-miR-30b-5p
XPO1 hsa-miR-30b-5p
HNRNPA3 hsa-miR-30b-5p
MAN1A2 hsa-miR-30c-5p
SLF2 hsa-miR-30c-5p
THAP12 hsa-miR-30c-5p
XPO1 hsa-miR-30c-5p
HNRNPA3 hsa-miR-30c-5p
MFSD14A hsa-miR-30d-5p
MAN1A2 hsa-miR-30d-5p
SLF2 hsa-miR-30d-5p
THAP12 hsa-miR-30d-5p
XPO1 hsa-miR-30d-5p
ZEB2 hsa-miR-30d-5p
HNRNPA3 hsa-miR-30d-5p
MFSD14A hsa-miR-30e-5p
MAN1A2 hsa-miR-30e-5p
SLF2 hsa-miR-30e-5p
THAP12 hsa-miR-30e-5p
XPO1 hsa-miR-30e-5p
ZEB2 hsa-miR-30e-5p
HNRNPA3 hsa-miR-30e-5p
MFSD14A hsa-miR-31-5p
KIF1BP hsa-miR-32-5p
LCOR hsa-miR-32-5p
KMT5B hsa-miR-32-5p
SRPRA hsa-miR-32-5p
ZEB2 hsa-miR-32-5p
MORC3 hsa-miR-32-5p
ERBIN hsa-miR-32-5p
PCMTD1 hsa-miR-32-5p
RGS3 hsa-miR-32-5p
MFSD14A hsa-miR-320a
SHTN1 hsa-miR-320a
KMT5B hsa-miR-320a
XPO1 hsa-miR-320a
MORC3 hsa-miR-320aTOMM70 hsa-miR-320aMFSD14A hsa-miR-320bSHTN1 hsa-miR-320bKMT5B hsa-miR-320bXPO1 hsa-miR-320bMORC3 hsa-miR-320bTOMM70 hsa-miR-320bMFSD14A hsa-miR-320cSHTN1 hsa-miR-320cKMT5B hsa-miR-320cXPO1 hsa-miR-320cMORC3 hsa-miR-320cTOMM70 hsa-miR-320cMFSD14A hsa-miR-320dSHTN1 hsa-miR-320dKMT5B hsa-miR-320dXPO1 hsa-miR-320dMORC3 hsa-miR-320dTOMM70 hsa-miR-320dSRPRA hsa-miR-326ERBIN hsa-miR-326SLF2 hsa-miR-329-3pSRPRA hsa-miR-330-5pERBIN hsa-miR-330-5pKMT5A hsa-miR-335-5pWAPL hsa-miR-338-3pPCNX1 hsa-miR-33a-5pERBIN hsa-miR-33a-5pPCNX1 hsa-miR-33b-5pERBIN hsa-miR-33b-5pMFSD14Ahsa-miR-340-5pLCOR hsa-miR-340-5pKMT5A hsa-miR-340-5pRGPD6 hsa-miR-340-5p
SRPRA hsa-miR-34a-5p
HSPA1B hsa-miR-34a-5p
SRPRA hsa-miR-34c-5p
HSPA1B hsa-miR-34c-5p
SLF2 hsa-miR-362-3p
KIF1BP hsa-miR-363-3p
LCOR hsa-miR-363-3p
KMT5B hsa-miR-363-3p
SRPRA hsa-miR-363-3p
ZEB2 hsa-miR-363-3p
MORC3 hsa-miR-363-3p
ERBIN hsa-miR-363-3p
PCMTD1 hsa-miR-363-3p
RGS3 hsa-miR-363-3p
EMSY hsa-miR-365a-3p
QTRT2 hsa-miR-365a-3p
KIF1BP hsa-miR-367-3p
LCOR hsa-miR-367-3p
KMT5B hsa-miR-367-3p
SRPRA hsa-miR-367-3p
ZEB2 hsa-miR-367-3p
MORC3 hsa-miR-367-3p
ERBIN hsa-miR-367-3p
PCMTD1 hsa-miR-367-3p
RGS3 hsa-miR-367-3p
PPP4R3A hsa-miR-371a-5p
LCOR hsa-miR-372-3p
KMT5B hsa-miR-372-3p
EMSY hsa-miR-372-3p
RUBCN hsa-miR-372-3p
LCOR hsa-miR-373-3p
KMT5B hsa-miR-373-3p
EMSY hsa-miR-373-3p
RUBCN hsa-miR-373-3pMFSD14A hsa-miR-374a-5pLCOR hsa-miR-374a-5pARID2hsa-miR-374a-5pANKRD12 hsa-miR-374a-5pMFSD14A hsa-miR-374b-5pLCOR hsa-miR-374b-5pARID2 hsa-miR-374b-5pANKRD12 hsa-miR-374b-5pLCOR hsa-miR-376c-3pWAPLhsa-miR-381-3pLCOR hsa-miR-381-3pKMT5B hsa-miR-381-3pEMSY hsa-miR-381-3pPPP4R3B hsa-miR-381-3pMFSD14B hsa-miR-381-3pKMT5A hsa-miR-382-5pMFSD14Bhsa-miR-382-5pMFSD14A hsa-miR-410-3pLCOR hsa-miR-410-3pEMSY hsa-miR-410-3pARID2 hsa-miR-410-3pCALM1 hsa-miR-410-3pNUDT3 hsa-miR-410-3pEMSY hsa-miR-421TOMM70 hsa-miR-421WAPL hsa-miR-424-5pSRPRA hsa-miR-424-5pCALM1 hsa-miR-424-5pRGPD6 hsa-miR-424-5pVPS50 hsa-miR-424-5pLCOR hsa-miR-425-5pPPP4R3A hsa-miR-425-5pWAPL hsa-miR-429SLF2 hsa-miR-429ZEB2 hsa-miR-429USF3 hsa-miR-429MFSD14B hsa-miR-431-5pCALM2 hsa-miR-448SRPRA hsa-miR-449aHSPA1B hsa-miR-449aSRPRA hsa-miR-449b-5pHSPA1B hsa-miR-449b-5pEMSY hsa-miR-451a
SLF2 hsa-miR-454-3p
UBC hsa-miR-454-3pFAM234A hsa-miR-454-3pANKRD12 hsa-miR-454-3pCALM2hsa-miR-454-3pZEB2 hsa-miR-454-3pERBIN hsa-miR-454-3pPRUNE1 hsa-miR-485-5pMFSD14A hsa-miR-488-3pLCOR hsa-miR-488-3pSHTN1 hsa-miR-494-3pEMSY hsa-miR-494-3pHNRNPA3 hsa-miR-494-3pPRUNE1 hsa-miR-495-3pEMSY hsa-miR-495-3pRGPD6hsa-miR-495-3pPRELID3B hsa-miR-495-3pGRK3 hsa-miR-495-3pWAPL hsa-miR-497-5pSRPRA hsa-miR-497-5pCALM1 hsa-miR-497-5pRGPD6 hsa-miR-497-5p
VPS50 hsa-miR-497-5pVPS41 hsa-miR-505-3pLCOR hsa-miR-506-3pMFSD14Ahsa-miR-519d-3pKMT5B hsa-miR-519d-3pEMSY hsa-miR-519d-3pTAOK3 hsa-miR-519d-3pUBC hsa-miR-519d-3pANKRD12 hsa-miR-519d-3pPCMTD1 hsa-miR-519d-3pLCOR hsa-miR-520a-3pKMT5B hsa-miR-520a-3pEMSY hsa-miR-520a-3pRUBCN hsa-miR-520a-3pLCORhsa-miR-520bKMT5B hsa-miR-520bEMSY hsa-miR-520bRUBCN hsa-miR-520bLCOR hsa-miR-520c-3pKMT5B hsa-miR-520c-3pEMSY hsa-miR-520c-3pRUBCN hsa-miR-520c-3pLCORhsa-miR-520d-3pKMT5B hsa-miR-520d-3pEMSY hsa-miR-520d-3pRUBCN hsa-miR-520d-3pLCOR hsa-miR-520eKMT5B hsa-miR-520eEMSY hsa-miR-520eRUBCN hsa-miR-520eMFSD14A hsa-miR-542-3pEMSY hsa-miR-543LCOR hsa-miR-590-5pMFSD14A hsa-miR-613THAP12 hsa-miR-613ARID2 hsa-miR-613CALM2 hsa-miR-613HNRNPA3 hsa-miR-613GRK3 hsa-miR-613EMSY hsa-miR-653-5pWAPL hsa-miR-7-5pARID2 hsa-miR-7-5pKMT5A hsa-miR-7-5pANKRD12 hsa-miR-7-5pUTP4 hsa-miR-758-3p
H3F3A hsa-miR-873-5p
NUP58 hsa-miR-874-3pPPP4R3A hsa-miR-874-3pMFSD14A hsa-miR-9-5pPRUNE1hsa-miR-9-5pSHTN1 hsa-miR-9-5pANKRD12 hsa-miR-9-5pFBXO16 hsa-miR-9-5pPTPAhsa-miR-9-5pKIF1BP hsa-miR-92a-3pLCOR hsa-miR-92a-3pKMT5B hsa-miR-92a-3pSRPRAhsa-miR-92a-3pZEB2 hsa-miR-92a-3pMORC3 hsa-miR-92a-3pERBIN hsa-miR-92a-3pPCMTD1 hsa-miR-92a-3pRGS3 hsa-miR-92a-3pKIF1BP hsa-miR-92b-3pLCOR hsa-miR-92b-3pKMT5B hsa-miR-92b-3pSRPRA hsa-miR-92b-3pZEB2 hsa-miR-92b-3pMORC3 hsa-miR-92b-3pERBIN hsa-miR-92b-3pPCMTD1 hsa-miR-92b-3pRGS3 hsa-miR-92b-3p
MFSD14A hsa-miR-93-5p
KMT5B hsa-miR-93-5p
EMSY hsa-miR-93-5p
TAOK3 hsa-miR-93-5p
UBC hsa-miR-93-5p
ANKRD12 hsa-miR-93-5p
PCMTD1 hsa-miR-93-5p
SLF2 hsa-miR-96-5p
PPP4R3A hsa-miR-96-5p
SLF2 hsa-miR-98-5p
INTS6L hsa-miR-98-5p171个靶向差异表达circRNA的miRNA，详情如下：circRNAmiRNA
hsa_circ_0000038hsa-let-7a-5p
hsa_circ_0000160hsa-let-7a-5p
hsa_circ_0000038hsa-let-7b-5p
hsa_circ_0000160hsa-let-7b-5p
hsa_circ_0000038hsa-let-7c-5p
hsa_circ_0000160hsa-let-7c-5p
hsa_circ_0000038hsa-let-7d-5p
hsa_circ_0000160hsa-let-7d-5p
hsa_circ_0000038hsa-let-7e-5p
hsa_circ_0000160hsa-let-7e-5p
hsa_circ_0000038hsa-let-7f-5p
hsa_circ_0000160hsa-let-7f-5p
hsa_circ_0000038hsa-let-7g-5p
hsa_circ_0000160hsa-let-7g-5p
hsa_circ_0000038hsa-let-7i-5p
hsa_circ_0000160hsa-let-7i-5p
hsa_circ_0001360hsa-miR-1-3phsa_circ_0000038hsa-miR-1193hsa_circ_0000160hsa-miR-122-5phsa_circ_0000639 hsa-miR-1245b-5phsa_circ_0000896 hsa-miR-1252-5phsa_circ_0001016 hsa-miR-1278hsa_circ_0001360 hsa-miR-128-3phsa_circ_0000038 hsa-miR-1287-5phsa_circ_0001163 hsa-miR-1294hsa_circ_0001016hsa-miR-130a-5phsa_circ_0000019 hsa-miR-1323hsa_circ_0000038 hsa-miR-137hsa_circ_0000722 hsa-miR-138-5phsa_circ_0001648 hsa-miR-141-3phsa_circ_0001309hsa-miR-145-5phsa_circ_0001163 hsa-miR-149-5phsa_circ_0000038 hsa-miR-15a-5phsa_circ_0000038 hsa-miR-15b-5phsa_circ_0000038 hsa-miR-16-5phsa_circ_0000019 hsa-miR-182-5phsa_circ_0000038 hsa-miR-184hsa_circ_0000043 hsa-miR-188-5phsa_circ_0000842 hsa-miR-18a-5phsa_circ_0000842 hsa-miR-18b-5phsa_circ_0000722 hsa-miR-1908-5phsa_circ_0000038 hsa-miR-1914-3phsa_circ_0000038 hsa-miR-195-5phsa_circ_0000311 hsa-miR-199a-3phsa_circ_0000311 hsa-miR-199b-3phsa_circ_0000842 hsa-miR-19a-3phsa_circ_0000842 hsa-miR-19b-3phsa_circ_0001648 hsa-miR-200a-3phsa_circ_0001360 hsa-miR-200b-3phsa_circ_0001360 hsa-miR-200c-3phsa_circ_0001540 hsa-miR-205-5phsa_circ_0001360 hsa-miR-206hsa_circ_0000043 hsa-miR-21-5phsa_circ_0000019 hsa-miR-2114-3phsa_circ_0001360hsa-miR-216a-3phsa_circ_0000160 hsa-miR-217hsa_circ_0000043 hsa-miR-219a-2-3phsa_circ_0000160 hsa-miR-219a-2-3phsa_circ_0000639 hsa-miR-223-3phsa_circ_0000311 hsa-miR-2278hsa_circ_0001163 hsa-miR-2355-3phsa_circ_0001939 hsa-miR-2355-3phsa_circ_0001016 hsa-miR-23a-3p
hsa_circ_0001016 hsa-miR-23b-3p
hsa_circ_0001016 hsa-miR-23chsa_circ_0000038 hsa-miR-2682-5phsa_circ_0001648 hsa-miR-28-5p
hsa_circ_0000896 hsa-miR-28-5p
hsa_circ_0001020 hsa-miR-29a-3p
hsa_circ_0001020 hsa-miR-29b-3p
hsa_circ_0001020 hsa-miR-29c-3phsa_circ_0001540 hsa-miR-3064-5phsa_circ_0000091 hsa-miR-30c-5phsa_circ_0000842 hsa-miR-3121-3phsa_circ_0000311hsa-miR-3129-5phsa_circ_0001648 hsa-miR-3139hsa_circ_0000896 hsa-miR-3139hsa_circ_0000639 hsa-miR-3142hsa_circ_0001309 hsa-miR-3145-3phsa_circ_0000639hsa-miR-3164hsa_circ_0000311 hsa-miR-3167hsa_circ_0000842 hsa-miR-3174hsa_circ_0000722 hsa-miR-3179hsa_circ_0000043 hsa-miR-324-5phsa_circ_0000722 hsa-miR-324-5phsa_circ_0000639 hsa-miR-326hsa_circ_0000639 hsa-miR-330-5phsa_circ_0000160 hsa-miR-33a-5phsa_circ_0000160 hsa-miR-33b-5phsa_circ_0000019hsa-miR-340-5phsa_circ_0000038 hsa-miR-34a-5phsa_circ_0000038 hsa-miR-34b-5phsa_circ_0000038 hsa-miR-34c-5phsa_circ_0000311 hsa-miR-3612hsa_circ_0000038 hsa-miR-3614-5phsa_circ_0000160 hsa-miR-3614-5phsa_circ_0001360 hsa-miR-3681-3phsa_circ_0000038 hsa-miR-370-3phsa_circ_0001680 hsa-miR-370-3phsa_circ_0000896 hsa-miR-374a-3phsa_circ_0000038 hsa-miR-374b-3phsa_circ_0000842hsa-miR-374c-5phsa_circ_0000311 hsa-miR-376a-3phsa_circ_0000311 hsa-miR-376b-3phsa_circ_0001648 hsa-miR-380-3phsa_circ_0001648 hsa-miR-382-5phsa_circ_0000842 hsa-miR-382-5phsa_circ_0001163 hsa-miR-3909hsa_circ_0000160 hsa-miR-421hsa_circ_0000038 hsa-miR-424-5phsa_circ_0001360 hsa-miR-429hsa_circ_0001939 hsa-miR-433-3phsa_circ_0000311 hsa-miR-4428hsa_circ_0000038 hsa-miR-4458hsa_circ_0000160 hsa-miR-4458hsa_circ_0000038 hsa-miR-449ahsa_circ_0000038 hsa-miR-449b-5phsa_circ_0000038 hsa-miR-449c-5phsa_circ_0000038 hsa-miR-4500hsa_circ_0000160 hsa-miR-4500hsa_circ_0000038 hsa-miR-452-5phsa_circ_0000038 hsa-miR-4676-3phsa_circ_0000896 hsa-miR-4731-5phsa_circ_0000842 hsa-miR-4735-3phsa_circ_0000038 hsa-miR-4761-5phsa_circ_0000842 hsa-miR-4761-5phsa_circ_0000091 hsa-miR-4766-3phsa_circ_0000019 hsa-miR-483-3phsa_circ_0001648 hsa-miR-489-3phsa_circ_0000038 hsa-miR-491-5phsa_circ_0000160 hsa-miR-493-5phsa_circ_0000842 hsa-miR-494-3phsa_circ_0001020 hsa-miR-496hsa_circ_0000038 hsa-miR-497-5phsa_circ_0001540 hsa-miR-500a-3phsa_circ_0000019hsa-miR-505-3phsa_circ_0001540 hsa-miR-505-3phsa_circ_0000091 hsa-miR-5094hsa_circ_0001939 hsa-miR-511-3phsa_circ_0000019 hsa-miR-513a-5phsa_circ_0001684 hsa-miR-514a-3phsa_circ_0000639 hsa-miR-514a-5phsa_circ_0000842 hsa-miR-514a-5phsa_circ_0001684 hsa-miR-514b-3phsa_circ_0000038 hsa-miR-516b-5phsa_circ_0000038 hsa-miR-5194hsa_circ_0001309 hsa-miR-5195-3phsa_circ_0000160 hsa-miR-526b-5phsa_circ_0001016 hsa-miR-526b-5phsa_circ_0001020 hsa-miR-526b-5phsa_circ_0000311 hsa-miR-543hsa_circ_0000842 hsa-miR-543hsa_circ_0000019 hsa-miR-548o-3phsa_circ_0000160 hsa-miR-556-5phsa_circ_0000043 hsa-miR-5579-3phsa_circ_0000043 hsa-miR-5581-3phsa_circ_0000842 hsa-miR-576-5phsa_circ_0001648 hsa-miR-579-3phsa_circ_0000043 hsa-miR-580-3phsa_circ_0000842 hsa-miR-582-3phsa_circ_0000043 hsa-miR-590-5phsa_circ_0001016 hsa-miR-599hsa_circ_0000639 hsa-miR-605-3phsa_circ_0001360 hsa-miR-613hsa_circ_0000038 hsa-miR-615-3phsa_circ_0000160 hsa-miR-624-5phsa_circ_0000091 hsa-miR-627-5phsa_circ_0001648 hsa-miR-642a-3phsa_circ_0001648 hsa-miR-642b-3phsa_circ_0000311 hsa-miR-650hsa_circ_0001540 hsa-miR-6504-5phsa_circ_0001684 hsa-miR-653-5phsa_circ_0000842 hsa-miR-655-3phsa_circ_0000722 hsa-miR-663ahsa_circ_0001648 hsa-miR-664b-3phsa_circ_0000019 hsa-miR-670-3phsa_circ_0001360 hsa-miR-671-5phsa_circ_0000038 hsa-miR-6738-5phsa_circ_0000722hsa-miR-6787-5phsa_circ_0000160 hsa-miR-6807-3phsa_circ_0000639 hsa-miR-6820-3phsa_circ_0000019 hsa-miR-6823-3phsa_circ_0000038 hsa-miR-6838-5phsa_circ_0001163 hsa-miR-6852-3phsa_circ_0000043 hsa-miR-6866-3phsa_circ_0000038 hsa-miR-6893-3phsa_circ_0001680 hsa-miR-6893-3phsa_circ_0000160 hsa-miR-7-5p
hsa_circ_0001648 hsa-miR-708-5p
hsa_circ_0000896hsa-miR-708-5p
hsa_circ_0000722hsa-miR-744-5phsa_circ_0000038hsa-miR-760
hsa_circ_0001360hsa-miR-802
hsa_circ_0001684hsa-miR-802
hsa_circ_0000311hsa-miR-876-5p
hsa_circ_0000038hsa-miR-892c-3p
hsa_circ_0000038hsa-miR-942-5p
hsa_circ_0000038hsa-miR-98-5p
hsa_circ_0000160hsa-miR-98-5p166个包含差异表达lncRNA结合位点的miRNA，详情如下：lncRNA miRNA
H3F3AP6 hsa-miR-96
H3F3AP6 hsa-miR-507
H3F3AP6 hsa-miR-1271
H3F3AP6 hsa-miR-182
H3F3AP6 hsa-miR-22
H3F3AP6 hsa-miR-22-3pH3F3AP6 hsa-miR-101
H3F3AP6 hsa-miR-101abTPM3P8 hsa-miR-9
TPM3P8 hsa-miR-9abTPM3P8 hsa-miR-150
TPM3P8 hsa-miR-5127
TPM3P8 hsa-miR-15abcTPM3P8 hsa-miR-16
TPM3P8 hsa-miR-16abcTPM3P8 hsa-miR-195
TPM3P8 hsa-miR-322
TPM3P8 hsa-miR-424
TPM3P8 hsa-miR-497
TPM3P8 hsa-miR-1907
TPM3P8 hsa-miR-182
TPM3P8 hsa-miR-205TPM3P8 hsa-miR-205abTPM3P8 hsa-miR-214TPM3P8 hsa-miR-761TPM3P8 hsa-miR-3619-5pTPM3P8 hsa-miR-218TPM3P8 hsa-miR-218aTPM3P8 hsa-miR-338TPM3P8 hsa-miR-338-3pTPM3P8 hsa-miR-34acTPM3P8 hsa-miR-34bc-5pTPM3P8hsa-miR-449abcTPM3P8 hsa-miR-449c-5pZNF322P1 hsa-miR-132ZNF322P1 hsa-miR-212ZNF322P1 hsa-miR-212-3pZNF322P1 hsa-miR-7ZNF322P1 hsa-miR-7abZNF322P1 hsa-miR-96ZNF322P1 hsa-miR-507ZNF322P1 hsa-miR-1271ZNF322P1 hsa-miR-135abZNF322P1hsa-miR-135a-5pZNF322P1 hsa-miR-138ZNF322P1 hsa-miR-138abZNF322P1 hsa-miR-139-5pZNF322P1 hsa-miR-143ZNF322P1 hsa-miR-1721ZNF322P1 hsa-miR-4770ZNF322P1hsa-miR-146acZNF322P1 hsa-miR-146b-5pZNF322P1 hsa-miR-148ab-3pZNF322P1 hsa-miR-152ZNF322P1 hsa-miR-150ZNF322P1 hsa-miR-5127ZNF322P1 hsa-miR-181abcdZNF322P1 hsa-miR-4262ZNF322P1 hsa-miR-199ab-5pZNF322P1 hsa-miR-19ab
ZNF322P1 hsa-miR-216a
ZNF322P1 hsa-miR-216bZNF322P1 hsa-miR-216b-5pZNF322P1 hsa-miR-221ZNF322P1 hsa-miR-222ZNF322P1 hsa-miR-222abZNF322P1 hsa-miR-1928ZNF322P1hsa-miR-122ZNF322P1 hsa-miR-122aZNF322P1 hsa-miR-1352ZNF322P1 hsa-miR-23abcZNF322P1 hsa-miR-23b-3pZNF322P1 hsa-miR-27abcZNF322P1 hsa-miR-27a-3pZNF322P1 hsa-miR-103aZNF322P1 hsa-miR-107ZNF322P1 hsa-miR-107abZNF322P1hsa-miR-124ZNF322P1 hsa-miR-124abZNF322P1 hsa-miR-506ZNF322P1 hsa-miR-33abZNF322P1 hsa-miR-33-5pZNF322P1 hsa-miR-383ZNF322P1 hsa-miR-125a-5pZNF322P1 hsa-miR-125b-5pZNF322P1 hsa-miR-351ZNF322P1 hsa-miR-670ZNF322P1hsa-miR-4319ZNF322P1 hsa-miR-128ZNF322P1 hsa-miR-128abZNF322P1 hsa-miR-129-5pZNF322P1 hsa-miR-129ab-5pRPL13AP7 hsa-miR-29abcdRPL21P119 hsa-miR-7RPL21P119 hsa-miR-7abRPL21P119 hsa-miR-9RPL21P119 hsa-miR-9abRPL21P119 hsa-miR-141RPL21P119 hsa-miR-200aRPL21P119 hsa-miR-145RPL21P119 hsa-miR-182RPL21P119 hsa-miR-204RPL21P119 hsa-miR-204bRPL21P119 hsa-miR-211RPL21P119hsa-miR-205RPL21P119 hsa-miR-205abRPL21P119 hsa-miR-101RPL21P119 hsa-miR-101abRPL21P119 hsa-miR-31RPL21P119 hsa-miR-338RPL21P119 hsa-miR-338-3pFTH1P20hsa-miR-96FTH1P20 hsa-miR-507FTH1P20 hsa-miR-1271FTH1P20 hsa-miR-135abFTH1P20hsa-miR-135a-5pFTH1P20 hsa-miR-145FTH1P20 hsa-miR-150FTH1P20 hsa-miR-5127FTH1P20 hsa-miR-182FTH1P20 hsa-miR-218FTH1P20 hsa-miR-218aFTH1P20 hsa-miR-223FTH1P20 hsa-miR-23abc
FTH1P20 hsa-miR-23b-3p
FTH1P20 hsa-miR-27abcFTH1P20 hsa-miR-27a-3pFTH1P20 hsa-miR-128FTH1P20hsa-miR-128abEEF1A1P11 hsa-miR-551aEEF1A1P11 hsa-miR-93EEF1A1P11 hsa-miR-93aEEF1A1P11 hsa-miR-105EEF1A1P11 hsa-miR-106aEEF1A1P11 hsa-miR-291a-3pEEF1A1P11 hsa-miR-294EEF1A1P11 hsa-miR-295EEF1A1P11 hsa-miR-302abcdeEEF1A1P11 hsa-miR-372EEF1A1P11 hsa-miR-373EEF1A1P11 hsa-miR-428EEF1A1P11 hsa-miR-519a
EEF1A1P11 hsa-miR-520beEEF1A1P11 hsa-miR-520acd-3pEEF1A1P11 hsa-miR-1378EEF1A1P11 hsa-miR-1420acEEF1A1P11 hsa-miR-96EEF1A1P11 hsa-miR-507EEF1A1P11 hsa-miR-1271EEF1A1P11 hsa-miR-138EEF1A1P11 hsa-miR-138abEEF1A1P11 hsa-miR-143EEF1A1P11 hsa-miR-1721EEF1A1P11 hsa-miR-4770EEF1A1P11 hsa-miR-153EEF1A1P11 hsa-miR-15abcEEF1A1P11 hsa-miR-16EEF1A1P11hsa-miR-16abcEEF1A1P11 hsa-miR-195EEF1A1P11 hsa-miR-322EEF1A1P11 hsa-miR-424EEF1A1P11 hsa-miR-497EEF1A1P11 hsa-miR-1907EEF1A1P11 hsa-miR-190EEF1A1P11hsa-miR-190abEEF1A1P11 hsa-miR-199ab-5pEEF1A1P11 hsa-miR-205EEF1A1P11 hsa-miR-205abEEF1A1P11 hsa-miR-21EEF1A1P11 hsa-miR-590-5pEEF1A1P11 hsa-miR-214EEF1A1P11 hsa-miR-761EEF1A1P11 hsa-miR-3619-5pEEF1A1P11 hsa-miR-223EEF1A1P11 hsa-miR-24EEF1A1P11 hsa-miR-24abEEF1A1P11 hsa-miR-24-3pEEF1A1P11hsa-miR-101EEF1A1P11 hsa-miR-101abEEF1A1P11 hsa-miR-103aEEF1A1P11 hsa-miR-107EEF1A1P11 hsa-miR-107abEEF1A1P11 hsa-miR-338EEF1A1P11 hsa-miR-338-3pEEF1A1P11 hsa-miR-383EEF1A1P11 hsa-miR-425EEF1A1P11 hsa-miR-425-5pEEF1A1P11hsa-miR-489EEF1A1P11 hsa-miR-129-5pEEF1A1P11 hsa-miR-129ab-5pRPS2P7 hsa-miR-148ab-3pRPS2P7 hsa-miR-152RPS2P7 hsa-miR-34acRPS2P7 hsa-miR-34bc-5pRPS2P7hsa-miR-449abcRPS2P7 hsa-miR-449c-5pRPS2P7 hsa-miR-490-3pH3F3BP1 hsa-miR-96H3F3BP1 hsa-miR-507H3F3BP1 hsa-miR-1271H3F3BP1 hsa-miR-182H3F3BP1 hsa-miR-22H3F3BP1 hsa-miR-22-3pH3F3BP1 hsa-miR-101H3F3BP1 hsa-miR-101abRPL9P7 hsa-miR-137RPL9P7 hsa-miR-137abRPL9P7 hsa-miR-191RPL9P7 hsa-miR-194RPL9P7 hsa-miR-199ab-5pRPL9P7 hsa-miR-1abRPL9P7 hsa-miR-206RPL9P7 hsa-miR-613RPL9P7 hsa-miR-205RPL9P7 hsa-miR-205abRPL9P7 hsa-miR-210RPL9P7 hsa-miR-25RPL9P7 hsa-miR-32RPL9P7 hsa-miR-92abcRPL9P7 hsa-miR-363RPL9P7 hsa-miR-363-3pRPL9P7 hsa-miR-367RPL7AP11 hsa-miR-133abcRPL7AP11 hsa-miR-17RPL7AP11 hsa-miR-17-5p
RPL7AP11 hsa-miR-20abRPL7AP11 hsa-miR-20b-5pRPL7AP11 hsa-miR-93RPL7AP11 hsa-miR-106abRPL7AP11 hsa-miR-427RPL7AP11 hsa-miR-518a-3pRPL7AP11hsa-miR-519dRPL7AP11 hsa-miR-199ab-5pRPL7AP11 hsa-miR-19abRPL7AP11 hsa-miR-204RPL7AP11 hsa-miR-204bRPL7AP11 hsa-miR-211RPL7AP11 hsa-miR-214RPL7AP11 hsa-miR-761RPL7AP11 hsa-miR-3619-5pRPL7AP11 hsa-miR-31PKD1P1 hsa-miR-503PKD1P1hsa-miR-7PKD1P1 hsa-miR-7abPKD1P1 hsa-miR-9PKD1P1 hsa-miR-9abPKD1P1 hsa-miR-135abPKD1P1 hsa-miR-135a-5pPKD1P1 hsa-miR-138PKD1P1 hsa-miR-138abPKD1P1 hsa-miR-146acPKD1P1 hsa-miR-146b-5pPKD1P1 hsa-miR-148ab-3pPKD1P1 hsa-miR-152PKD1P1 hsa-miR-150PKD1P1 hsa-miR-5127PKD1P1 hsa-miR-15abcPKD1P1 hsa-miR-16PKD1P1 hsa-miR-16abcPKD1P1 hsa-miR-195PKD1P1 hsa-miR-322PKD1P1 hsa-miR-424PKD1P1 hsa-miR-497PKD1P1 hsa-miR-1907PKD1P1 hsa-miR-184PKD1P1 hsa-miR-let-7PKD1P1 hsa-miR-98PKD1P1 hsa-miR-4458PKD1P1 hsa-miR-4500PKD1P1 hsa-miR-18abPKD1P1 hsa-miR-4735-3pPKD1P1 hsa-miR-193PKD1P1 hsa-miR-193bPKD1P1 hsa-miR-193a-3pPKD1P1 hsa-miR-205PKD1P1 hsa-miR-205abPKD1P1 hsa-miR-214PKD1P1hsa-miR-761PKD1P1 hsa-miR-3619-5pPKD1P1 hsa-miR-218PKD1P1 hsa-miR-218aPKD1P1hsa-miR-219-5pPKD1P1 hsa-miR-508PKD1P1 hsa-miR-508-3pPKD1P1 hsa-miR-4782-3pPKD1P1 hsa-miR-24PKD1P1 hsa-miR-24abPKD1P1 hsa-miR-24-3pPKD1P1 hsa-miR-29abcdPKD1P1 hsa-miR-31PKD1P1 hsa-miR-103aPKD1P1 hsa-miR-107PKD1P1 hsa-miR-107ab
PKD1P1 hsa-miR-338PKD1P1 hsa-miR-338-3p
PKD1P1 hsa-miR-34acPKD1P1 hsa-miR-34bc-5pPKD1P1 hsa-miR-449abcPKD1P1hsa-miR-449c-5pPKD1P1 hsa-miR-125a-5pPKD1P1 hsa-miR-125b-5pPKD1P1 hsa-miR-351PKD1P1 hsa-miR-670PKD1P1 hsa-miR-4319PKD1P1 hsa-miR-128PKD1P1 hsa-miR-128abPKD1P1 hsa-miR-490-3pEEF1A1P9 hsa-miR-503EEF1A1P9 hsa-miR-551aEEF1A1P9hsa-miR-93EEF1A1P9 hsa-miR-93aEEF1A1P9 hsa-miR-105EEF1A1P9 hsa-miR-106aEEF1A1P9 hsa-miR-291a-3pEEF1A1P9 hsa-miR-294EEF1A1P9 hsa-miR-295EEF1A1P9hsa-miR-302abcdeEEF1A1P9 hsa-miR-372EEF1A1P9 hsa-miR-373EEF1A1P9 hsa-miR-428EEF1A1P9 hsa-miR-519a
EEF1A1P9 hsa-miR-520beEEF1A1P9 hsa-miR-520acd-3pEEF1A1P9 hsa-miR-1378EEF1A1P9 hsa-miR-1420acEEF1A1P9 hsa-miR-135abEEF1A1P9 hsa-miR-135a-5pEEF1A1P9 hsa-miR-138EEF1A1P9 hsa-miR-138abEEF1A1P9 hsa-miR-140EEF1A1P9 hsa-miR-140-5pEEF1A1P9 hsa-miR-876-3pEEF1A1P9 hsa-miR-1244EEF1A1P9 hsa-miR-143EEF1A1P9 hsa-miR-1721EEF1A1P9 hsa-miR-4770EEF1A1P9 hsa-miR-146acEEF1A1P9hsa-miR-146b-5pEEF1A1P9 hsa-miR-15abcEEF1A1P9 hsa-miR-16EEF1A1P9 hsa-miR-16abcEEF1A1P9 hsa-miR-195EEF1A1P9 hsa-miR-322EEF1A1P9 hsa-miR-424EEF1A1P9hsa-miR-497EEF1A1P9 hsa-miR-1907EEF1A1P9 hsa-miR-190EEF1A1P9 hsa-miR-190abEEF1A1P9 hsa-miR-199ab-5pEEF1A1P9 hsa-miR-205EEF1A1P9 hsa-miR-205abEEF1A1P9 hsa-miR-214EEF1A1P9 hsa-miR-761EEF1A1P9 hsa-miR-3619-5pEEF1A1P9hsa-miR-223EEF1A1P9 hsa-miR-24EEF1A1P9 hsa-miR-24ab
EEF1A1P9 hsa-miR-24-3p
EEF1A1P9 hsa-miR-27abcEEF1A1P9 hsa-miR-27a-3pEEF1A1P9 hsa-miR-101EEF1A1P9 hsa-miR-101abEEF1A1P9 hsa-miR-103aEEF1A1P9 hsa-miR-107EEF1A1P9hsa-miR-107abEEF1A1P9 hsa-miR-338EEF1A1P9 hsa-miR-338-3pEEF1A1P9 hsa-miR-383EEF1A1P9 hsa-miR-425EEF1A1P9 hsa-miR-425-5pEEF1A1P9 hsa-miR-489EEF1A1P9hsa-miR-128EEF1A1P9 hsa-miR-128ab
EEF1A1P9 hsa-miR-129-5pEEF1A1P9 hsa-miR-129ab-5pHMGN2P41 hsa-miR-133abcHMGN2P41 hsa-miR-9HMGN2P41 hsa-miR-9abHMGN2P41 hsa-miR-96HMGN2P41 hsa-miR-507HMGN2P41 hsa-miR-1271HMGN2P41 hsa-miR-182HMGN2P41 hsa-miR-let-7HMGN2P41 hsa-miR-98HMGN2P41 hsa-miR-4458HMGN2P41 hsa-miR-4500HMGN2P41 hsa-miR-204HMGN2P41 hsa-miR-204bHMGN2P41 hsa-miR-211HMGN2P41 hsa-miR-205HMGN2P41hsa-miR-205abHMGN2P41 hsa-miR-338HMGN2P41 hsa-miR-338-3pHMGN2P41 hsa-miR-34acHMGN2P41 hsa-miR-34bc-5p
HMGN2P41 hsa-miR-449abc
HMGN2P41 hsa-miR-449c-5p

Claims (10)

1.血浆外泌体标志物作为动脉粥样硬化诊断标志物的应用，其特征在于，所述血浆外泌体标志物为环状hsa_circ_0001360和/或环状hsa_circ_0001360的基因表达的产品。
2.检测如权利要求1所述的环状hsa_circ_0001360的基因表达的产品在制备诊断粥样动脉硬化的工具中的应用。
3.根据权利要求2所述的应用，其特征在于，所述检测环状hsa_circ_0001360的基因表达的产品包括检测环状hsa_circ_0001360表达水平的产品。
4.根据权利要求3所述的应用，其特征在于，与健康人群相比，受试者样本中hsa_circ_0001360的表达水平升高，则诊断该受试者为动脉粥样硬化患者。
5.根据权利要求4所述的应用，其特征在于，所述受试者样本的来源是血浆外泌体。
6.根据权利要求2所述的应用，其特征在于，所述产品为能够结合hsa_circ_0001360的核酸，所述核酸包括特异性扩增hsa_circ_0001360的引物，所述引物的序列如SEQ ID NO：1和SEQ ID NO：2所示。
7.一种获取权利要求1-6任一项所述的血浆外泌体标志物的方法，其特征在于，包括以下步骤：
S1.构建CircRNA/lncRNA-miRNA-mRNA调控网络；
S2.样本中血液外泌体circRNA的筛选；
S3.样本中血液外泌体circRNA的表达验证。
8.根据权利要求7所述的方法，其特征在于，所述构建CircRNA/lncRNA-miRNA-mRNA调控网络的方法包括：首先通过ENCORI、TargetScan和miRanda数据库预测靶向差异mRNA的miRNA和靶向差异表达circRNA的miRNA，其次从miRcode数据库中获取包含差异表达lncRNA结合位点的miRNA，基于竞争性内源性调控网络选择重叠的miRNAs，最后利用“ggalluvial”包构建参与受试者样本的血液外泌体的CircRNA/lncRNA-miRNA-mRNA调控网络并进行可视化。
9.一种探针，其特征在于，所述探针能够检测权利要求4所述的受试者样本中hsa_circ_0001360的表达水平。
10.一种包括权利要求9所述探针的试剂盒，其特征在于所述试剂盒可以用于诊断动脉粥样硬化。
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CN117330752A (zh) * 2023-11-17 2024-01-02 首都医科大学 Slc14a1作为标志物在制备评估结直肠癌肝转移风险和/或预后情况产品中的应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117330752A (zh) * 2023-11-17 2024-01-02 首都医科大学 Slc14a1作为标志物在制备评估结直肠癌肝转移风险和/或预后情况产品中的应用

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