CN116809130A - Microfluidic chip and joint, interface and microfluidic system thereof - Google Patents
Microfluidic chip and joint, interface and microfluidic system thereof Download PDFInfo
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- CN116809130A CN116809130A CN202211100550.1A CN202211100550A CN116809130A CN 116809130 A CN116809130 A CN 116809130A CN 202211100550 A CN202211100550 A CN 202211100550A CN 116809130 A CN116809130 A CN 116809130A
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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Abstract
The invention provides a microfluidic chip and a joint, an interface and a microfluidic system thereof. The connector comprises a fluid connector body, wherein one end of the fluid connector body along the connection direction is provided with connector connectors and connector fluid connectors which are arranged in an array; the connector connecting piece is provided with a connector positioning and connecting structure, the fluid connector main body is also provided with through holes which are arranged in an array and penetrate through the fluid connector main body, the through holes are used for arranging fluid inflow connecting pipes and fluid outflow connecting pipes in an array, the through holes are in one-to-one correspondence with the connector fluid connecting ports, and the openings of the through holes are formed or connected with the connector fluid connecting ports. Through the through holes and the joint fluid connectors which are arranged in the array and reasonably controlled to be selectively connected with each culture unit, the operation complexity of the multi-communication channels can be reduced, and the conditions of reagent leakage, environmental pollution and the like can be effectively avoided.
Description
Technical Field
The invention belongs to the technical field of microfluidic chips, and particularly relates to a microfluidic chip and a joint, an interface and a microfluidic system thereof.
Background
The microfluidic chip technology (Microfluidics) integrates basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes on a micron-scale chip, and automatically completes the whole analysis process. Because of its great potential in biological, chemical, medical and other fields, it has been developed into a new research field where the disciplines of biology, chemistry, medicine, fluids, electronics, materials, machinery and the like are crossed.
"Lab-on-a-chip" is an transliteration of "Lab-on-a-chip", and microfluidic technology is an important component of the Lab-on-a-chip. Laboratory-on-chip techniques include non-flowing static micro-experiment systems where the flow of fluid is not controlled, such as microarray chips, gene chips, etc., and microfluidic systems where precise movement of fluid can be controlled. At the beginning of the 90 s of the 20 th century, microfluidic technology was developed on the basis of microelectronics, micromechanics, bioengineering and nanotechnology, becoming a representative technology in lab-on-a-chip technology. The micro-fluidic chip is an important embodiment of micro-fluidic technology, and the main characteristics of the micro-fluidic chip are that three key words of micro-fluidic chip, flow and control chip are that the micro-fluidic chip can integrate an operation unit of a sample and a cell into a chip with a size of a few square centimeters or less, and the flow is that the chip forms a network by micro-channels so as to control fluid to penetrate through the whole system. "control" is characterized by that it can be real-time monitored for sample preparation, reaction, separation, detection, cell culture and separation. Microfluidic chip technology is becoming a technology platform for replacing various functions of conventional chemical or biological laboratories.
Microfluidic technology has been developed for nearly 30 years, and has been widely used in the fields of drug screening, disease development and diagnosis, environmental poison monitoring, aerospace and the like. Microfluidic technology has become one of the most active advanced technologies in the 21 st century, and has fast development, wide application and influence on aspects of life.
In the related art, a hard tube or a pagoda joint is adopted to adhere to the microfluidic chip, so that external reagents can be introduced into the chip or flow out of the chip.
However, in the above connection method, if different reagents are required for different culture units in the chip, a plurality of communication channels are required to be provided at this time, and obviously, the above connection method increases the complexity of multi-channel operation, and the leakage of reagents, environmental pollution and the like are also easily caused by unstable adhesion.
Disclosure of Invention
The invention aims at solving at least one of the technical problems existing in the prior art and provides a microfluidic chip, a joint, an interface and a microfluidic system thereof.
In one aspect of the present invention, there is provided a joint for a microfluidic chip, including a fluid joint body; wherein,,
one end of the fluid joint main body along the connecting direction is provided with joint connectors and joint fluid connectors which are arranged in an array; the joint connecting piece is provided with a joint positioning and connecting structure, when the joint connecting piece establishes connection, the joint positioning and connecting structure positions the joint fluid connecting port to a position aligned with the flow path connection, and after the joint connecting piece establishes connection, the joint positioning and connecting structure positions the joint fluid connecting port to a position established with the flow path connection;
the fluid connector body is also provided with through holes which are arranged in an array and penetrate through the fluid connector body, the through holes are used for arranging fluid inflow connecting pipes and fluid outflow connecting pipes in an array, the through holes are in one-to-one correspondence with the connector fluid connecting ports, and the openings of the through holes are formed or connected with the connector fluid connecting ports.
Optionally, the fluid inflow connecting pipe and the fluid outflow connecting pipe pass through and/or are fixed to the through holes and are connected to the joint fluid connection ports;
the fluid inflow connecting pipe is used for conveying fluid into the flow path after the joint connector is connected; the fluid outflow connecting pipe is used for outputting fluid in a flow path after the joint connection is established.
Optionally, the joint fluid connection port is located inside the joint connector, and the joint positioning connection structure protrudes out of the fluid joint body along the connection direction.
Optionally, the joint positioning connection structure is an asymmetric structure.
Optionally, the fluid connector body further includes a sealing member matching the shape of the connector fluid connection port for sealing around the interface of the flow path established by the connector fluid connection port after the connector connection member establishes a connection.
Optionally, the fluid connector body further comprises a sealing member matching the shape of the connector fluid interface for sealing around the interface of the connector fluid connection port establishment flow path after the connector connection member establishes a connection.
Optionally, the fluid connector body further comprises a fastener for fastening the connection between the fluid inflow connection pipe and the flow path and the connection between the fluid outflow connection pipe and the flow path after the connector connection is established.
Optionally, the fastener includes an operation portion and a threaded connection portion connected to the operation portion, where the operation portion is located on a side of the fluid joint body facing away from the joint connection member, so as to adjust a fastening degree when the joint connection member is connected by operating the operation portion.
Optionally, the through holes include a first through hole and a second through hole;
the fluid joint main body comprises a first joint main body piece and a second joint main body piece which is arranged opposite to the first joint main body piece and connected with the first joint main body piece, the first through holes are arranged in an array and penetrate through the first joint main body piece, and the second through holes penetrate through the second joint main body piece;
the fluid inflow connecting pipe and the fluid outflow connecting pipe each comprise a first connecting pipe and a second connecting pipe; the first end of the first connecting pipe penetrates through and/or is fixed to the first through hole, the second end of the first connecting pipe penetrates through the first end of the corresponding second connecting pipe, and the second connecting pipe penetrates through the second through hole.
Optionally, the interval range between two adjacent rows of through holes is 2 mm-4 mm; and/or the number of the groups of groups,
the distance between two adjacent through holes is 2 mm-2.4 mm.
Optionally, the outer diameter of the first connecting pipe ranges from 0.8mm to 1.2mm, the inner diameter of the first connecting pipe ranges from 0.5mm to 0.9mm, and the length of the first connecting pipe ranges from 8mm to 12mm;
the outer diameter range of the second connecting pipe is 1.7 mm-2.1 mm, and the inner diameter range of the second connecting pipe is 0.6 mm-1.0 mm.
In another aspect of the present invention, there is provided an interface for a microfluidic chip, comprising a fluid interface body, wherein,
the fluid interface main body is provided with an interface connecting piece and interface fluid connecting ports which are arranged in an array manner at one end along the connecting direction, the interface connecting piece is provided with an interface positioning connecting structure, and when the interface connecting piece establishes connection, the interface positioning connecting structure positions the interface fluid connecting ports to the positions aligned with the connection of the flow paths; after the interface connector establishes connection, the interface positioning connection structure positions the interface fluid connection port to a position for establishing flow path connection;
the fluid interface main body is also provided with through holes penetrating through the fluid interface main body, and the through holes are in one-to-one correspondence with the interface fluid connectors; the through hole is formed or connected with the interface fluid interface along an opening of one end of the connection direction.
Optionally, the interface fluid connection port is located inside the interface connector, the connection structure of the interface connector is concave in the fluid interface main body along the connection direction, and the connection structure of the interface connector is an asymmetric structure.
In another aspect of the present invention, there is provided a microfluidic chip including the interface for a microfluidic chip described above, the microfluidic chip further including a plurality of fluid connection channels, the plurality of fluid connection channels being selectively connected to one end of the through-hole facing away from the connection direction.
Optionally, the plurality of fluid connection channels are located at different layers of the microfluidic chip, and each through hole correspondingly connected penetrates to a corresponding layer.
In another aspect of the present invention, there is provided a microfluidic system, including a microfluidic chip, and further including a connector for the microfluidic chip, wherein the connector for the microfluidic chip adopts the connector described above, the fluid connector body is connected to the microfluidic chip, and the fluid inflow connection pipe and the fluid outflow connection pipe are both connected to the outside.
According to the microfluidic chip and the joint, the joint and the microfluidic system, through the joint fluid connectors which are arranged in an array and the corresponding communicated through holes which are arranged on the joint main body, the introduced fluid which is arranged in the array can flow into the connecting pipe and the fluid can flow out of the connecting pipe, and a plurality of communication channels which are connected with a plurality of flow channels in the microfluidic chip can be formed, so that the tissue organ can be cultured in a circulating perfusion way. Therefore, the joint for the microfluidic chip of the embodiment can reasonably control each through hole to be selectively connected with each culture unit, can reduce the operation complexity of multiple communication channels, integrates the multiple through holes on the joint main body, and can effectively avoid the occurrence of the conditions of reagent leakage, environmental pollution and the like. In addition, the connector head connector also has a connector positioning structure which can facilitate an operator to quickly position the connector fluid connection port to a position aligned with the flow path and, after connection is established, can facilitate an operator to position the connector fluid connection port to a position where the flow path connection is established.
Drawings
Fig. 1 is a schematic structural view of a joint for a microfluidic chip according to a first embodiment of the present invention;
FIG. 2 is an exploded view of a joint for a microfluidic chip according to a second embodiment of the present invention;
FIG. 3 is a schematic diagram showing the distribution of through holes according to a third embodiment of the present invention;
fig. 4 is a schematic structural diagram of a microfluidic system according to a fourth embodiment of the present invention;
fig. 5 is an exploded view of a microfluidic system according to a fifth embodiment of the present invention.
Detailed Description
The present invention will be described in further detail below with reference to the drawings and detailed description for the purpose of better understanding of the technical solution of the present invention to those skilled in the art.
First, a joint for a microfluidic chip according to an embodiment of the present invention will be described with reference to fig. 1 to 4.
As shown in fig. 1 and 2, a joint 100 for a microfluidic chip includes a fluid joint body 110, and the fluid joint body 110 has a joint connector at one end in a connection direction and joint fluid connection ports 130 arranged in an array. The joint connector has a joint positioning connection structure 120, when the joint connector establishes a connection, the joint positioning connection structure 120 positions the joint fluid connection port 130 to a position aligned with the flow path connection, and after the joint connector establishes a connection, the joint positioning connection structure 120 positions the joint fluid connection port 130 to a position establishing the flow path connection.
As shown in fig. 1, the fluid connector body 110 further has through holes 111 arranged in an array and penetrating through the fluid connector body 110, for arranging fluid inflow connection pipes and fluid outflow connection pipes in an array, the through holes 111 are in one-to-one correspondence with the connector fluid connection ports 130, and openings of the through holes 111 are formed or connected to the connector fluid connection ports 130.
According to the joint for the microfluidic chip, through the joint fluid connectors which are arranged in an array and the through holes which are correspondingly communicated and are formed in the fluid joint main body, fluid can be correspondingly led into the fluid inflow connecting pipe in an array arrangement mode and fluid can be led out through the fluid outflow connecting pipe, a plurality of communication channels which are connected with a plurality of flow channels in the microfluidic chip can be formed, and therefore circulation perfusion culture of tissue and organs is completed. Therefore, the joint for the microfluidic chip of the embodiment can reasonably control each through hole to be selectively connected with each culture unit, can reduce the operation complexity of multiple communication channels, integrates the multiple through holes on the joint main body, and can effectively avoid the occurrence of the conditions of reagent leakage, environmental pollution and the like. In addition, the connector has a connector positioning structure that can facilitate an operator in quickly positioning the connector fluid connection port to a position that aligns the flow path, and after establishing the connection, can facilitate an operator in positioning the connector fluid connection port to a position that establishes the flow path connection.
As shown in fig. 1 and 2, the joint 100 for a microfluidic chip further includes a fluid inflow connection pipe 140 and a fluid outflow connection pipe 150, which are passed through and/or fixed to the through-hole 111, and are connected to the joint fluid connection port 130. The fluid inflow connection pipe 140 is used for delivering fluid into a flow path after the joint connection is established; the fluid outflow connection pipe 150 is used to output fluid in a flow path after the joint connection is established. It should be noted that, the position of the residual fluid flowing out of the connecting pipe 150 of the fluid flowing in connecting pipe 140 may be determined according to the connection relationship with the fluid channel in the microfluidic chip, for example, in fig. 1, six fluid flowing in connecting pipes may be left, six fluid flowing out connecting pipes may be right; or the upper row may be a fluid inflow connection pipe and the lower row may be a fluid outflow connection pipe, and the positional relationship between the fluid inflow connection pipe and the fluid outflow connection pipe is not particularly limited in the present disclosure.
The joint for the microfluidic chip of the embodiment integrates a plurality of fluid inflow connecting pipes and fluid outflow connecting pipes which are arranged in an array on the fluid joint main body, and enables the fluid joint main body to be directly connected with the microfluidic chip through the joint connecting piece so as to form a plurality of communication channels connected with a plurality of flow channels inside the microfluidic chip, and reagents can be introduced into or led out of the culture unit through the fluid inflow connecting pipes or the fluid outflow connecting pipes, so that the tissue organ circulation perfusion culture is completed. Therefore, the joint for the microfluidic chip of the embodiment can reasonably control the fluid inflow connecting pipe and the fluid outflow connecting pipe to be selectively connected with each culture unit, can reduce the operation complexity of multiple communication channels, integrates the fluid inflow connecting pipe and the fluid outflow connecting pipe on the joint main body, and can further effectively avoid the occurrence of the conditions of reagent leakage, environmental pollution and the like.
As shown in fig. 1 and 2, the joint fluid connection port 130 is located inside the joint connector, and the joint positioning connection structure 120 protrudes from the fluid joint body 110 along the connection direction, and correspondingly, in the interface for a microfluidic chip, there is an interface positioning connection structure recessed along the connection direction, so that the joint positioning connection structure 120 can be inserted into the interface positioning connection structure. Of course, in addition to this, the connector positioning and connecting structure 120 may be recessed in the fluid connector main body 110 along the connection direction, and accordingly, in the interface for a microfluidic chip, there is an interface positioning and connecting structure protruding along the connection direction, which may be specifically determined according to actual needs, and this embodiment is not limited thereto.
According to the embodiment, through the concave-convex design of the joint positioning connection structure, the joint positioning connection structure can be matched with the interface positioning connection structure of the concave-convex design on the microfluidic chip in a plugging manner, so that the efficiency of establishing a fluid passage can be improved, and meanwhile, through the clamping connection of the protrusions and the grooves, fluid leakage is avoided.
As shown in fig. 1 and 2, the joint positioning connection structure 120 is an asymmetric structure. The structure of the asymmetric design can enable operators to accurately position the joint fluid connection ports to the positions where the aligned flow paths are connected, avoid the occurrence of wrong connection, and realize foolproof design; on the other hand, it is also possible to facilitate the operator in discriminating the flow path direction, that is, in discriminating the fluid inflow connection pipe and the fluid outflow connection pipe.
As one example, as shown in fig. 1, the joint positioning connection structure 120 may have a trapezoid shape. Of course, other shapes of the joint positioning connection structure 120 are possible, and the present embodiment is not limited thereto.
As shown in fig. 1, the fluid connector main body 110 further includes a sealing member 160 having a shape matching that of the connector fluid connection port 130, so as to seal the periphery of the interface of the flow path established by the connector fluid connection port 130 after the connector connection is established, thereby further avoiding leakage of the reagent, environmental pollution, and the like.
It should be noted that, the specific material of the seal 160 is not limited, and preferably, the seal 160 may be formed by using an elastic material, for example, the seal 160 may be one of a silicone seal, a rubber seal, a latex seal, and a polydimethylsiloxane seal. Of course, other materials may be selected to form the seal 160 according to actual needs, and the present embodiment is not limited thereto.
As shown in fig. 1 and 2, the fluid coupling body 110 further includes a fastening member 170 for fastening the connection between the fluid inflow connection pipe 140 and the flow path and the connection between the fluid outflow connection pipe 150 and the flow path after the coupling connection is established.
Specifically, as shown in fig. 1, the fastening member 170 includes an operation portion 171 and a screw connection portion 172 connected to the operation portion 171, the operation portion 171 being located at a side of the fluid joint main body 110 facing away from the joint connection member, so as to adjust a fastening degree of the joint connection member when the joint connection member is established by operating the operation portion 171.
As shown in fig. 1, the through-hole 111 includes a first through-hole 111a and a second through-hole 111b. The fluid connector main body 110 includes a first connector main body member 112 and a second connector main body member 113 disposed opposite to and connected to the first connector main body member 112, the first through holes 111a are arranged in an array and penetrate through the first connector main body member 112, and the second through holes 111b penetrate through the second connector main body member 113.
As shown in fig. 2, the fluid inflow connection pipe 140 and the fluid outflow connection pipe 150 each include a first connection pipe A1 and a second connection pipe A2. The first end of the first connecting pipe A1 passes through and/or is fixed to the first through hole 111a, the second end of the first connecting pipe A1 passes through the first end of the corresponding second connecting pipe A2, and the second connecting pipe A2 passes through the second through hole 111b.
The micro-fluidic chip of this embodiment is with connecting, fluid inflow connecting pipe and fluid outflow connecting pipe adopt split type design's pipeline structure, and first connecting pipe can adopt hardness higher material, for example stainless steel material, and hose material such as silica gel can be adopted to the second connecting pipe, and first connecting pipe hardness is higher can guarantee to connect and micro-fluidic chip fastening connection time non-deformable, avoids reagent to leak, and hose material such as silica gel is adopted to the second connecting pipe, can improve the adaptation degree with external connection spare to under the circumstances of being connected with peristaltic pump, can improve the efficiency of pumping in reagent.
Note that the first through holes and the second through holes may be provided in one-to-one correspondence, and, as shown in fig. 1, the second through holes may correspond to a plurality of first through holes, that is, a plurality of second connection pipes A2 may be provided in the same second through hole. Of course, the first through holes may correspond to a plurality of second through holes, and the present embodiment is not limited thereto.
As shown in fig. 1, 2 and 3, the interval between two adjacent rows of through holes 111 ranges from 2mm to 4mm; the distance between two adjacent through holes 111 ranges from 2mm to 2.4mm. Of course, besides these, those skilled in the art can reasonably design the interval between two adjacent rows of through holes and the interval between two adjacent through holes according to actual needs, and the embodiment is not particularly limited.
Referring to fig. 3 together, in practical use, it is assumed that the microfluidic chip 300 includes two culture units therein, and at this time, the N1 and N3 through holes can be used as the inlet and outlet of one of the culture units to complete the cyclic perfusion culture of the tissue and organ in the culture unit. The N6 and N4 through holes can be used as the inlet and outlet of another culture unit to finish the circulatory perfusion culture of the tissue and organ in the culture unit. In addition, it is assumed that three culture units are included in the microfluidic chip 300, and at this time, the N1 and N2 through holes, the N3 and N4 through holes, and the N5 and N6 through holes may be used as the inlets and outlets of the three culture units, respectively, so as to complete the cyclic perfusion culture of the internal tissue organ. In addition, it is assumed that six culture units are included in the microfluidic chip 300, and at this time, N1 and T1 through holes, N2 and T2 through holes, N3 and T3 through holes, N4 and T4 through holes, N5 and T5 through holes, and N6 and T6 through holes may be used as the inlets and outlets of the six culture units, respectively, thereby completing the cyclic perfusion culture of the internal tissue and organ. In addition, those skilled in the art may reasonably design other access ways according to actual needs, and the present embodiment is not limited thereto.
Illustratively, the first connecting tube may have an outer diameter in the range of 0.8mm to 1.2mm, preferably 1mm. The first connecting tube may have an inner diameter in the range of 0.5mm to 0.9mm, preferably 0.7mm. The length of the first connecting tube may range from 8mm to 12mm, preferably 10mm. The first connecting tube may be a hard tube of stainless steel, PC, PTFE or other materials.
Illustratively, the second connecting tube has an outer diameter in the range of 1.7mm to 2.1mm, preferably 1.9mm. The second connecting tube has an inner diameter in the range of 0.6mm to 1.0mm, preferably 0.8mm. The second connecting pipe can be made of elastic materials such as silica gel, latex, PP, PE and the like.
In addition, the fluid connector body 110 may be manufactured by using a hard high-quality material such as PMMA, PC, COC, COM, PTFE, PICK or by injection molding.
In another aspect of the present invention, as shown in fig. 5, there is provided an interface 200 for a microfluidic chip, wherein the interface 200 is used for connecting with the connector 100 for a microfluidic chip described above. Alternatively, the microfluidic chip interface 200 may be disposed on the microfluidic chip 300. The microfluidic chip interface 200 may include a fluid interface main body 210, where one end of the fluid interface main body along a connection direction has an interface connector and interface fluid connection ports 220 arranged in an array, the interface connector has an interface positioning connection structure 230, and when the interface connector establishes a connection, the interface positioning connection structure 230 positions the interface fluid connection ports 220 to a position aligned with a flow path connection; after the interface connector establishes a connection, the interface positioning connection structure 230 positions the interface fluid connection port to a position where a flow path connection is established. The fluid interface body 210 further has a through hole 211 penetrating through the fluid interface body 210, where the through holes 211 are in one-to-one correspondence with the interface fluid connection ports 220; the through-hole 211 is formed or connected to the interface fluid port 220 in an opening at one end in the connection direction.
Illustratively, as shown in fig. 5, the interface fluid connection port 220 is located inside the interface connector, the connection structure of the interface connector is recessed in the fluid interface body along the connection direction, and the interface positioning connection structure 230 is an asymmetric structure.
In another aspect of the present invention, as shown in fig. 5, there is provided a microfluidic chip 300 including the interface for a microfluidic chip described above, where the microfluidic chip 300 further includes a plurality of fluid connection channels 310, and the plurality of fluid connection channels 310 are selectively connected to one end of the through hole 211 facing away from the connection direction.
Illustratively, the plurality of fluid connection channels 310 are located at different levels of the microfluidic chip 300, and each through-hole 211 correspondingly connected penetrates to a corresponding level.
In another aspect of the present invention, as shown in fig. 4 and fig. 5, a microfluidic system 400 is provided, including a microfluidic chip 300, and the microfluidic chip connector is the microfluidic chip connector 100 described above, and the detailed description of the microfluidic chip connector is omitted herein. The fluid connector body 110 is connected to the microfluidic chip 300, and the fluid inflow connection pipe and the fluid outflow connection pipe are connected to the outside (e.g., a reagent source, etc.).
According to the microfluidic system, the connector for the microfluidic chip is adopted, and through the connector fluid connectors arranged in an array and the corresponding communicated through holes arranged on the connector main body, introduced fluid arranged in an array can flow into the connecting pipe and fluid flows out of the connecting pipe, so that a plurality of communication channels connected with a plurality of flow channels inside the microfluidic chip can be formed, and the tissue organ can be circularly perfused and cultured. Therefore, the joint for the microfluidic chip of the embodiment can reasonably control each through hole to be selectively connected with each culture unit, can reduce the operation complexity of multiple communication channels, integrates the multiple through holes on the joint main body, and can effectively avoid the occurrence of the conditions of reagent leakage, environmental pollution and the like. In addition, the connector head connector also has a connector positioning structure which can facilitate an operator to quickly position the connector fluid connection port to a position aligned with the flow path and, after connection is established, can facilitate an operator to position the connector fluid connection port to a position where the flow path connection is established.
It is to be understood that the above embodiments are merely illustrative of the application of the principles of the present invention, but not in limitation thereof. Various modifications and improvements may be made by those skilled in the art without departing from the spirit and substance of the invention, and are also considered to be within the scope of the invention.
Claims (15)
1. The joint for the micro-fluidic chip is characterized by comprising a fluid joint main body; wherein,,
one end of the fluid joint main body along the connecting direction is provided with joint connectors and joint fluid connectors which are arranged in an array; the joint connecting piece is provided with a joint positioning and connecting structure, when the joint connecting piece establishes connection, the joint positioning and connecting structure positions the joint fluid connecting port to a position aligned with the flow path connection, and after the joint connecting piece establishes connection, the joint positioning and connecting structure positions the joint fluid connecting port to a position established with the flow path connection;
the fluid connector body is also provided with through holes which are arranged in an array and penetrate through the fluid connector body, the through holes are used for arranging fluid inflow connecting pipes and fluid outflow connecting pipes in an array, the through holes are in one-to-one correspondence with the connector fluid connecting ports, and the openings of the through holes are formed or connected with the connector fluid connecting ports.
2. The joint for a microfluidic chip according to claim 1, further comprising a fluid inflow connecting pipe and a fluid outflow connecting pipe which pass through and/or are fixed to the through-hole and are connected to the joint fluid connection port;
the fluid inflow connecting pipe is used for conveying fluid into the flow path after the joint connector is connected; the fluid outflow connecting pipe is used for outputting fluid in a flow path after the joint connection is established.
3. The joint for a microfluidic chip according to claim 1 or 2, wherein the joint fluid connection port is located inside the joint connector, and the joint positioning connection structure protrudes from the fluid joint body in a connection direction.
4. A connector for a microfluidic chip according to claim 3, wherein the connector positioning and connecting structure is an asymmetric structure.
5. The connector for a microfluidic chip according to claim 1 or 2, wherein the fluid connector body further comprises a sealing member having a shape matching that of the connector fluid connection port for sealing around the interface of the flow path established by the connector fluid connection port after the connector connection member establishes connection.
6. The joint for a microfluidic chip according to claim 1 or 2, wherein the fluid joint body further comprises a fastener for fastening a connection between the fluid inflow connection pipe and the flow path and a connection between the fluid outflow connection pipe and the flow path after the joint connection is established.
7. The connector for a microfluidic chip according to claim 6, wherein the fastener comprises an operation portion and a screw connection portion connected to the operation portion, the operation portion being located at a side of the fluid connector body facing away from the connector connection member, so that the degree of fastening when the connector connection member is established is adjusted by operating the operation portion.
8. The joint for a microfluidic chip according to claim 2, wherein the through hole includes a first through hole and a second through hole;
the fluid joint main body comprises a first joint main body piece and a second joint main body piece which is arranged opposite to the first joint main body piece and connected with the first joint main body piece, the first through holes are arranged in an array and penetrate through the first joint main body piece, and the second through holes penetrate through the second joint main body piece;
the fluid inflow connecting pipe and the fluid outflow connecting pipe each comprise a first connecting pipe and a second connecting pipe; the first end of the first connecting pipe penetrates through and/or is fixed to the first through hole, the second end of the first connecting pipe penetrates through the first end of the corresponding second connecting pipe, and the second connecting pipe penetrates through the second through hole.
9. The joint for a microfluidic chip according to claim 1, wherein a spacing between two adjacent rows of through holes ranges from 2mm to 4mm; and/or the number of the groups of groups,
the distance between two adjacent through holes is 2 mm-2.4 mm.
10. The joint for a microfluidic chip according to any one of claims 1 to 9, wherein the outer diameter of the first connecting tube ranges from 0.8mm to 1.2mm, the inner diameter of the first connecting tube ranges from 0.5mm to 0.9mm, and the length of the first connecting tube ranges from 8mm to 12mm;
the outer diameter range of the second connecting pipe is 1.7 mm-2.1 mm, and the inner diameter range of the second connecting pipe is 0.6 mm-1.0 mm.
11. An interface for a microfluidic chip is characterized by comprising a fluid interface main body, wherein,
the fluid interface main body is provided with an interface connecting piece and interface fluid connecting ports which are arranged in an array manner at one end along the connecting direction, the interface connecting piece is provided with an interface positioning connecting structure, and when the interface connecting piece establishes connection, the interface positioning connecting structure positions the interface fluid connecting ports to the positions aligned with the connection of the flow paths; after the interface connector establishes connection, the interface positioning connection structure positions the interface fluid connection port to a position for establishing flow path connection;
the fluid interface main body is also provided with through holes penetrating through the fluid interface main body, and the through holes are in one-to-one correspondence with the interface fluid connectors; the through hole is formed or connected with the interface fluid interface along an opening of one end of the connection direction.
12. The interface for a microfluidic chip according to claim 11, wherein the interface fluid connection port is located inside the interface connector, the connection structure of the interface connector is recessed in the fluid interface body along the connection direction, and the connection structure of the interface connector is an asymmetric structure.
13. A microfluidic chip comprising the interface for a microfluidic chip according to claims 11-12, wherein the microfluidic chip further comprises a plurality of fluid connection channels, the plurality of fluid connection channels being selectively connected to one end of the through-hole facing away from the connection direction.
14. The microfluidic chip according to claim 13, wherein the plurality of fluid connection channels are located at different levels of the microfluidic chip, each through-hole of a corresponding connection penetrating to a corresponding level.
15. A microfluidic system comprising a microfluidic chip, wherein the microfluidic system further comprises a joint for the microfluidic chip, the joint for the microfluidic chip adopts the joint according to any one of claims 1 to 10, the fluid joint body is connected with the microfluidic chip, and the fluid inflow connecting pipe and the fluid outflow connecting pipe are both connected with the outside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211100550.1A CN116809130A (en) | 2022-09-09 | 2022-09-09 | Microfluidic chip and joint, interface and microfluidic system thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211100550.1A CN116809130A (en) | 2022-09-09 | 2022-09-09 | Microfluidic chip and joint, interface and microfluidic system thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116809130A true CN116809130A (en) | 2023-09-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211100550.1A Pending CN116809130A (en) | 2022-09-09 | 2022-09-09 | Microfluidic chip and joint, interface and microfluidic system thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116809130A (en) |
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2022
- 2022-09-09 CN CN202211100550.1A patent/CN116809130A/en active Pending
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