CN116808342A - Bioreactor for liver failure - Google Patents

Bioreactor for liver failure Download PDF

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Publication number
CN116808342A
CN116808342A CN202310777305.2A CN202310777305A CN116808342A CN 116808342 A CN116808342 A CN 116808342A CN 202310777305 A CN202310777305 A CN 202310777305A CN 116808342 A CN116808342 A CN 116808342A
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China
Prior art keywords
water bath
blood
tube
bioreactor
communicated
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CN202310777305.2A
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Chinese (zh)
Inventor
兰青
周菊
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Yunnan University YNU
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Yunnan University YNU
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Priority to CN202310777305.2A priority Critical patent/CN116808342A/en
Publication of CN116808342A publication Critical patent/CN116808342A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3639—Blood pressure control, pressure transducers specially adapted therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3623—Means for actively controlling temperature of blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3627—Degassing devices; Buffer reservoirs; Drip chambers; Blood filters
    • A61M1/3633—Blood component filters, e.g. leukocyte filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/14—Pressurized fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/0021—Special media to be introduced, removed or treated removed from and reintroduced into the body, e.g. after treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Vascular Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Anesthesiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Cardiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • External Artificial Organs (AREA)

Abstract

The invention discloses a bioreactor for liver failure, which comprises a water bath shell, a blood filter, a plasma separator and a bioreactor, wherein liver cells are cultured in the bioreactor, the blood filter, the plasma separator and the bioreactor are connected through a plurality of pipes, a filtering placing groove, a separating placing groove and an exchanging placing groove are sequentially formed in the front side of the water bath shell, an infusion tube groove for regularly carding the pipes is further formed in the front side of the water bath shell, and a first peristaltic pump and a second peristaltic pump are arranged on the water bath shell; the rear side of water bath casing is provided with the heating cabinet, is provided with electric heating pipe in the heating cabinet, and the water pump is installed to the rear side of water bath casing, and the entry end of water pump and water bath casing pass through the drinking-water pipe intercommunication, and the exit end and the heating cabinet intercommunication of water pump, the heating cabinet passes through the water injection pipe and communicates with the water bath casing. The invention can carry out regular carding on the used pipes, and can carry out water bath heat preservation on the whole system, thereby preventing the hypothermia of patients caused by blood returning to human bodies.

Description

Bioreactor for liver failure
Technical Field
The invention relates to the technical field of medical appliances, in particular to a bioreactor for liver failure.
Background
Liver failure is a serious liver dysfunction, usually caused by the inability of the liver to normally metabolize substances and maintain homeostasis in the body. At present, the main method for treating liver failure is to carry out liver transplantation, but the problems of insufficient liver source, high operation risk and the like limit the application range of the liver transplantation. Therefore, the development of bioreactors capable of replacing liver functions has become a research hotspot.
Existing liver failure bioreactors typically require the use of a large number of tubes for connecting the different components, but the arrangement of the tubes is often messy due to lack of carding. In addition, the existing bioreactor generally only can perform local water bath heat preservation, so that liver cells keep activity at a proper temperature for substance exchange, but the rest parts lack water bath heat preservation, so that the temperature of blood after being isolated is reduced, and the condition of hypothermia of a patient is easily caused after the blood returns to a human body again.
Disclosure of Invention
The invention aims to provide a bioreactor for liver failure, which can carry out regular carding on used pipes, and can also carry out water bath heat preservation on the whole system so as to prevent hypothermia of a patient caused by blood returning to a human body.
The technical aim of the invention is realized by the following technical scheme:
the bioreactor for liver failure comprises a water bath shell, a blood filter, a plasma separator and a bioreactor, wherein hepatocytes are cultured in the bioreactor, a filtering placing groove matched with the shape of the blood filter, a separating placing groove matched with the shape of the plasma separator and an exchanging placing groove matched with the shape of the bioreactor are sequentially formed in the front side of the water bath shell from left to right, a first peristaltic pump is arranged on the left side of the filtering placing groove of the water bath shell, and a second peristaltic pump is arranged between the separating placing groove and the exchanging placing groove of the water bath shell;
the upper end of the blood filter is communicated with a detachable blood inlet pipe, the waste liquid outlet end of the blood filter is communicated with a detachable waste liquid pipe, the blood outlet end of the blood filter is communicated with a detachable blood filtering pipe, the free end of the blood filtering pipe is detachably connected with the inlet end of the plasma separator, the outlet end of the blood cell outlet end of the blood separator is communicated with a detachable blood cell pipe, the plasma outlet end of the blood separator is communicated with a detachable plasma pipe, the free end of the plasma pipe is communicated with the inlet end of the bioreactor, the outlet end of the bioreactor is communicated with an exchange pipe communicated with the blood cell pipe, and the free end of the plasma pipe is communicated with a blood transfusion pipe;
the front side of the water bath shell is provided with a transfusion tube groove matched with the blood inlet tube, the waste liquid tube, the blood filtering tube, the blood cell tube, the plasma tube, the exchange tube and the blood transfusion tube, the transfusion tube groove is communicated with a first peristaltic pump and a second peristaltic pump, so that the blood inlet tube can penetrate into the first peristaltic pump, and the plasma tube can penetrate through the second peristaltic pump;
the water bath is characterized in that a heating box is arranged on the rear side of the water bath shell, constant-temperature water is sealed in the water bath shell, an electric heating pipe is arranged in the heating box, a water pump is arranged on the rear side of the water bath shell, the inlet end of the water pump is communicated with the water bath shell through a water suction pipe, the outlet end of the water pump is communicated with the heating box, the heating box is communicated with the water bath shell through a water injection pipe, a temperature sensor is arranged in the water bath shell, and an operation panel is arranged on one side of the water bath shell.
By adopting the technical scheme, when a patient with liver failure needs to be treated, a blood inlet pipe is connected with an arterial blood vessel of the patient, a blood delivery pipe is connected with a venous blood vessel of the patient, a waste liquid pipe is connected with a liquid collecting bag, a first peristaltic pump works, blood is pumped out of the body, redundant waste and redundant water in the blood are filtered after entering a blood filter, the filtered waste and redundant water flow out of the blood filter pipe, the filtered waste and redundant water are discharged from the waste liquid pipe, the blood flows into a plasma separator along the blood filter pipe, blood cells are separated from plasma, the blood cells flow out of the blood cell pipe, the plasma flows out of the plasma pipe, and enters a bioreactor under the action of a second peristaltic pump, after the plasma enters the bioreactor, the plasma passes through a semipermeable membrane pipe in the bioreactor, the plasma exchanges substances with liver cells through the semipermeable membrane pipe, the liver cells simulate the functions of liver, metabolic drugs, synthetic proteins are carried out, blood components are regulated and the like, and then the plasma flows out from the exchange pipe and the blood cells into the blood cell pipe to be mixed with the blood cell pipe, and finally returns to the venous blood vessel of the patient; the water pump and the heating pipe work, so that constant temperature water in the water bath shell can be circularly heated, the water bath shell is always kept at about 37 ℃, and the phenomenon that the temperature of human body is too low due to the fact that the blood temperature is reduced and then returns to the human body is avoided.
The invention is further provided with: the middle part intercommunication of advance blood vessel has the syringe, the syringe intercommunication has detachable anticoagulation syringe, the front side of water bath casing seted up with syringe matched with bifurcated pipe groove, the front side of water bath casing seted up with anticoagulation syringe matched with syringe standing groove, the water bath casing in the top of syringe standing groove is provided with and is used for promoting anticoagulation syringe's flexible motor.
By adopting the technical scheme, the telescopic motor can push the anticoagulant injector to automatically inject the anticoagulant, so that the blood coagulation is prevented.
The invention is further provided with: the blood inlet tube, the plasma tube and the middle part of blood transfusion tube all are provided with the pressure measurement drip chamber, water bath casing's front side seted up with each pressure measurement drip chamber matched with drip chamber groove, each pressure measurement drip chamber all communicates there is the pressure measurement intubate, water bath casing has all seted up the pressure measurement jack at the edge of each drip chamber groove, water bath casing is interior all to be provided with in the rear end of each pressure measurement jack with corresponding pressure measurement intubate matched with pressure sensor.
Through adopting above-mentioned technical scheme, there is air in the pressure measurement dropping funnel, blood or plasma get into in the pressure measurement dropping funnel, and the pressure of blood or plasma is detected by pressure sensor through the air in the pressure measurement intubate, and the in-process blood or plasma's of being convenient for real-time supervision pressure to the operating condition of first peristaltic pump and second peristaltic pump is convenient for adjust.
The invention is further provided with: the left side and the right side of the opening end of the filtering placing groove and the separating placing groove are respectively provided with an elastic rubber baffle strip.
The invention is further provided with: the cell culture device comprises a bioreactor, and is characterized in that hollow flow cavities are formed in two ends in the bioreactor, a cell culture section is arranged in the middle of the bioreactor, a plurality of semi-permeable membrane tubes which are tubular and are communicated with the corresponding hollow flow cavities in two ends are arranged in the cell culture section, a cell culture medium is arranged outside each semi-permeable membrane tube in the cell culture section, and liver cells are arranged on the outer side of each semi-permeable membrane tube.
Compared with the prior art, the invention has the following beneficial effects:
firstly, the front side of the water bath shell is provided with the grooves for placing the blood filter, the plasma separator and the bioreactor and the infusion tube grooves for regularly carding the tubes, so that mess caused by too much lack of carding of the tubes can be prevented;
secondly, the whole system can be subjected to water bath heat preservation through the water bath shell, so that the whole system can be kept at the temperature of about 37 ℃ in the use process, liver cells can not be kept active at a proper temperature for substance exchange, the temperature of blood is not reduced after the blood leaves the body, and the condition of hypothermia after the blood returns to a human body is avoided.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a bioreactor system for liver failure;
FIG. 3 is a partial sectional view for showing the internal structure of the bioreactor;
FIG. 4 is a schematic view showing the overall structure of the front side of the water bath housing;
FIG. 5 is a view for showing the internal structure of the water bath housing;
fig. 6 is used to show the heating box and water pump at the rear side of the water bath housing.
In the figure: 1. a water bath shell; 11. a filtering placing groove; 12. a separation placing groove; 13. exchanging the placing groove; 14. an elastic rubber barrier strip; 15. a first peristaltic pump; 16. a second peristaltic pump; 17. an infusion tube groove; 18. a bifurcated pipe groove; 19. a syringe placement groove; 110. a dropping funnel groove; 111. a pressure measuring jack; 2. a blood filter; 3. a plasma separator; 4. a bioreactor; 41. a hollow flow chamber; 42 cell culture sections; 43. a semipermeable membrane tube; 51. entering a blood vessel; 52. a waste liquid pipe; 53. a blood filtration tube; 54. a blood cell tube; 55. a plasma tube; 56. exchange the tube; 57. a blood transfusion tube; 58. measuring pressure and dropping the bucket; 59. a pressure measuring cannula; 6. a syringe; 61. an anticoagulation syringe; 62. a telescopic motor; 7. a heating box; 71. an electric heating tube; 72. a water pump; 73. a water pumping pipe; 74. a water injection pipe; 81. a pressure sensor; 82. a temperature sensor; 9. an operation panel.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
In the description of the present invention, it should be understood that the directions or positional relationships indicated by the terms "upper", "lower", "left", "right", "front", "rear", "inner", "outer", etc., are based on the directions or positional relationships shown in the drawings, are merely for convenience of description and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
1-6, a bioreactor for liver failure includes a water bath housing 1, a blood filter 2, a plasma separator 3, and a bioreactor 4. The two ends in the bioreactor 4 are provided with a section of hollow flow cavity 41, the middle part in the bioreactor 4 is provided with a section of cell culture section 42, a plurality of semipermeable membrane tubes 43 which are vertically arranged and are tubular in shape and are communicated with the corresponding hollow flow cavity 41 at the two ends are arranged in the cell culture section 42, a cell culture medium is arranged outside each semipermeable membrane tube 43 in the cell culture section 42, and liver cells are arranged outside each semipermeable membrane tube 43.
The front side of the water bath shell 1 is provided with a filtering placing groove 11 which is matched with the shape of the blood filter 2, a separating placing groove 12 which is matched with the shape of the plasma separator 3 and an exchanging placing groove 13 which is matched with the shape of the bioreactor 4, wherein the left side and the right side of the opening ends of the filtering placing groove 11, the separating placing groove 12 and the exchanging placing groove 13 are respectively provided with an elastic rubber barrier strip 14, so that the blood filter 2, the plasma separator 3 or the bioreactor 4 can be conveniently placed in the water bath shell without being pulled by external force and cannot fall off. The water bath shell 1 is provided with a first peristaltic pump 15 at the left side of the filtering placing groove 11, and the water bath shell 1 is provided with a second peristaltic pump 16 between the separating placing groove 12 and the exchanging placing groove 13.
The upper end of the blood filter 2 is communicated with a detachable blood inlet tube 51, the middle part of the blood inlet tube 51 is communicated with an injection tube 6, the injection tube 6 is communicated with a detachable anticoagulation syringe 61, and the anticoagulation syringe 61 is used for injecting anticoagulants and preventing blood coagulation; the waste liquid outlet end of the blood filter 2 is communicated with a detachable waste liquid pipe 52, filtered waste liquid is discharged from the waste liquid pipe 52, the blood outlet end of the blood filter 2 is communicated with a detachable blood filter pipe 53, the free end of the blood filter pipe 53 is detachably connected with the inlet end of the plasma separator 3, the outlet end of the blood cell outlet end of the blood separator is communicated with a detachable blood cell pipe 54, the plasma outlet end of the blood separator is communicated with a detachable plasma pipe 55, the free end of the plasma pipe 55 is communicated with the inlet end of the bioreactor 4, the outlet end of the bioreactor 4 is communicated with an exchange pipe 56 communicated with the blood cell pipe 54, and the free end of the plasma pipe 55 is communicated with a blood transfusion pipe 57.
The front side of the water bath shell 1 is provided with a transfusion tube groove 17 matched with a blood inlet tube 51, a waste liquid tube 52, a blood filter tube 53, a blood cell tube 54, a plasma tube 55, an exchange tube 56 and a blood transfusion tube 57, the transfusion tube groove 17 is communicated with a first peristaltic pump 15 and a second peristaltic pump 16, so that the blood inlet tube 51 can penetrate into the first peristaltic pump 15, the plasma tube 55 can penetrate through the second peristaltic pump 16, and the transfusion tube groove 17 is used for regulating the tubes to prevent the tubes from being arranged in disorder; the front side of the water bath shell 1 is provided with a bifurcated pipe groove 18 matched with the injection pipe 6, the front side of the water bath shell 1 is provided with a syringe placing groove 19 matched with the anticoagulation syringe 61, the water bath shell 1 is provided with a telescopic motor 62 used for pushing the anticoagulation syringe 61 above the syringe placing groove 19, and the anticoagulation syringe 61 is pushed by the telescopic motor 62 to automatically inject the anticoagulation agent into blood.
The middle parts of the blood inlet pipe 51, the blood plasma pipe 55 and the blood transfusion pipe 57 are all provided with a pressure measuring drip chamber 58, a drip chamber groove 110 matched with each pressure measuring drip chamber 58 is formed in the front side of the water bath shell 1, each pressure measuring drip chamber 58 is communicated with a pressure measuring insertion pipe 59, a pressure measuring insertion hole 111 is formed in the edge of each drip chamber groove 110 of the water bath shell 1, a pressure sensor 81 matched with the corresponding pressure measuring insertion pipe 59 is arranged at the rear end of each pressure measuring insertion hole 111 in the water bath shell 1, air is arranged in the pressure measuring drip chamber 58, blood or blood plasma enters the pressure measuring drip chamber 58, and the pressure of the blood or the blood plasma is detected by the pressure sensor 81 through the air in the pressure measuring insertion pipe 59, so that the pressure of the blood or the blood plasma in the process of real-time monitoring is convenient to adjust.
The rear side of the water bath shell 1 is provided with a heating box 7, the inside of the water bath shell 1 is sealed with constant temperature water, two electric heating pipes 71 are arranged in the heating box 7, a water pump 72 is arranged at the rear side of the water bath shell 1, the inlet end of the water pump 72 is communicated with the water bath shell 1 through a water suction pipe 73, the outlet end of the water pump 72 is communicated with the heating box 7, the heating box 7 is communicated with the water bath shell 1 through a water injection pipe 74, a temperature sensor 82 is arranged in the water bath shell 1, and the constant temperature water in the water bath shell 1 is circularly heated in the heating box 7 through the water pump 72, so that the water bath shell 1 is always maintained at about 37 ℃. One side of the water bath housing 1 is provided with an operation panel 9, and the entire system is controlled by the operation panel 9.
Working principle: when a patient with liver failure needs to be treated, the blood inlet pipe 51 is connected with an arterial blood vessel of the patient, the blood outlet pipe 57 is connected with a venous blood vessel of the patient, the waste liquid pipe 52 is connected with a liquid collecting bag, the first peristaltic pump 15 works, blood is pumped out of the body, anticoagulant is injected into the blood to prevent blood coagulation after passing through the anticoagulation syringe 61, redundant waste and redundant water in the blood are filtered after entering the blood filter 2, the filtered waste and redundant water flow out of the blood filter pipe 53, the filtered waste and redundant water are discharged from the waste liquid pipe 52, the blood flows into the plasma separator 3 along the blood filter pipe 53 to separate blood cells from plasma, the blood cells flow out of the blood cell pipe 54, the plasma flows out of the plasma pipe 55 and enters the bioreactor 4 under the action of the second peristaltic pump 16, the plasma passes through the semipermeable membrane pipe 43 in the inner part of the bioreactor 4, the plasma is subjected to substance exchange with liver cells through the semipermeable membrane pipe 43, the liver cells simulate liver functions, metabolic drugs, synthetic proteins, blood components and the like are regulated, and the plasma flows out of the exchange pipe 56 and the blood cells are finally mixed with the blood cells 54 into the blood cell pipe 54, and finally flows back into the vein of the patient; the pressure sensor 81 is used for monitoring the pressure of blood or plasma in the process in real time by the three pressure measuring dropping hoppers 58 in the process so as to adjust the working states of the first peristaltic pump 15 and the second peristaltic pump 16; the water pump 72 and the heating pipe work to circularly heat constant temperature water in the water bath shell 1, so that the water bath shell 1 is always kept at about 37 ℃, and the phenomenon that the temperature of the human body is too low due to the fact that the blood temperature is reduced and then returns to the human body is avoided.
The present embodiment is only for explanation of the present invention and is not to be construed as limiting the present invention, and modifications to the present embodiment, which may not creatively contribute to the present invention as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present invention.

Claims (5)

1. A bioreactor for liver failure, which comprises a water bath shell (1), a blood filter (2), a plasma separator (3) and a bioreactor (4), and is characterized in that: hepatocytes are cultured in the bioreactor (4), a filtering placing groove (11) matched with the shape of the blood filter (2), a separating placing groove (12) matched with the shape of the plasma separator (3) and an exchanging placing groove (13) matched with the shape of the bioreactor (4) are sequentially formed in the front side of the water bath shell (1), a first peristaltic pump (15) is arranged on the left side of the filtering placing groove (11) of the water bath shell (1), and a second peristaltic pump (16) is arranged between the separating placing groove (12) and the exchanging placing groove (13) of the water bath shell (1);
the upper end of the blood filter (2) is communicated with a detachable blood inlet tube (51), the waste liquid outlet end of the blood filter (2) is communicated with a detachable waste liquid tube (52), the blood outlet end of the blood filter (2) is communicated with a detachable blood filter tube (53), the free end of the blood filter tube (53) is detachably connected with the inlet end of the plasma separator (3), the outlet end of the blood cell outlet end of the blood separator is communicated with a detachable blood cell tube (54), the plasma outlet end of the blood separator is communicated with a detachable plasma tube (55), the free end of the plasma tube (55) is communicated with the inlet end of the bioreactor (4), the outlet end of the bioreactor (4) is communicated with an exchange tube (56) communicated with the blood cell tube (54), and the free end of the plasma tube (55) is communicated with a blood transfusion tube (57);
the front side of the water bath shell (1) is provided with a transfusion tube groove (17) matched with the blood inlet tube (51), the waste liquid tube (52), the blood filtering tube (53), the blood cell tube (54), the plasma tube (55), the exchange tube (56) and the blood transfusion tube (57), the transfusion tube groove (17) is communicated with a first peristaltic pump (15) and a second peristaltic pump (16), so that the blood inlet tube (51) can penetrate into the first peristaltic pump (15), and the plasma tube (55) can penetrate through the second peristaltic pump (16);
the water bath shell comprises a water bath shell body (1), wherein a heating box (7) is arranged at the rear side of the water bath shell body (1), constant-temperature water is sealed in the water bath shell body (1), an electric heating pipe (71) is arranged in the heating box (7), a water pump (72) is arranged at the rear side of the water bath shell body (1), an inlet end of the water pump (72) is communicated with the water bath shell body (1) through a water suction pipe (73), an outlet end of the water pump (72) is communicated with the heating box (7), the heating box (7) is communicated with the water bath shell body (1) through a water injection pipe (74), a temperature sensor (82) is arranged in the water bath shell body (1), and an operation panel (9) is arranged on one side of the water bath shell body (1).
2. A bioreactor for liver failure as in claim 1, wherein: the middle part intercommunication of advance blood vessel (51) has syringe (6), syringe (6) intercommunication has detachable anticoagulation syringe (61), the front side of water bath casing (1) seted up with syringe (6) matched with bifurcated tube groove (18), the front side of water bath casing (1) seted up with anticoagulation syringe (61) matched with syringe standing groove (19), water bath casing (1) in the top of syringe standing groove (19) is provided with and is used for promoting anticoagulation syringe (61) flexible motor (62).
3. A bioreactor for liver failure as in claim 1, wherein: the blood inlet tube (51), the blood plasma tube (55) and the middle part of blood transfusion tube (57) all are provided with pressure measurement drip chamber (58), water bath casing (1) the front side seted up with each pressure measurement drip chamber (58) matched with drip chamber groove (110), each pressure measurement drip chamber (58) all communicate and have pressure measurement intubate (59), water bath casing (1) all has seted up pressure measurement jack (111) in the edge of each drip chamber (110), water bath casing (1) in the rear end of each pressure measurement jack (111) all be provided with corresponding pressure measurement intubate (59) matched with pressure sensor (81).
4. A bioreactor for liver failure as in claim 1, wherein: the filtering placing groove (11), the separating placing groove (12) and the left and right sides of the opening end of the exchanging placing groove (13) are respectively provided with an elastic rubber baffle strip (14).
5. A bioreactor for liver failure as in claim 1, wherein: the cell culture device is characterized in that hollow flow cavities (41) are formed in two ends in the bioreactor (4), a cell culture section (42) is arranged in the middle of the bioreactor (4), a plurality of semi-permeable membrane tubes (43) which are tubular and are communicated with the corresponding hollow flow cavities (41) are arranged in the cell culture section (42), cell culture mediums are arranged outside each semi-permeable membrane tube (43) in the cell culture section (42), and hepatocytes are arranged outside each semi-permeable membrane tube (43).
CN202310777305.2A 2023-06-28 2023-06-28 Bioreactor for liver failure Pending CN116808342A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310777305.2A CN116808342A (en) 2023-06-28 2023-06-28 Bioreactor for liver failure

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310777305.2A CN116808342A (en) 2023-06-28 2023-06-28 Bioreactor for liver failure

Publications (1)

Publication Number Publication Date
CN116808342A true CN116808342A (en) 2023-09-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310777305.2A Pending CN116808342A (en) 2023-06-28 2023-06-28 Bioreactor for liver failure

Country Status (1)

Country Link
CN (1) CN116808342A (en)

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