CN116763860A - Method for separating total flavonoids from stems or leaves of Ampelopsis grossedentata and preparation and application of emulsifiable paste - Google Patents
Method for separating total flavonoids from stems or leaves of Ampelopsis grossedentata and preparation and application of emulsifiable paste Download PDFInfo
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- CN116763860A CN116763860A CN202310937129.4A CN202310937129A CN116763860A CN 116763860 A CN116763860 A CN 116763860A CN 202310937129 A CN202310937129 A CN 202310937129A CN 116763860 A CN116763860 A CN 116763860A
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- vine tea
- leaves
- stems
- total flavonoids
- powder
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Abstract
The invention discloses a method for separating vine tea stems or leaves from total flavonoids and preparation and application of cream, belongs to the technical field of traditional Chinese medicine pharmacy, and provides a method for separating vine tea stems or leaves from total flavonoids extract, preparation of cream containing vine tea stems or leaves from total flavonoids extract, and application of the cream containing vine tea stems or leaves from total flavonoids extract in preparation of medicines for treating and/or preventing psoriasis vulgaris. After the stem or leaf total flavone extract emulsifiable paste of the vine tea intervenes and treats the Balb/c mice induced by imiquimod, the weight of the mice is not obviously affected, the psoriasis symptoms of the Balb/c mice are obviously improved, the PASI score is reduced, a large amount of infiltration of inflammatory cells in dermis is reduced, abnormal proliferation of epidermal cells is inhibited, and oxidative stress injury is relieved. Therefore, the vine tea stem or leaf total flavone extract can be used for preparing medicines for treating and/or preventing psoriasis.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to a vine tea stem or leaf total flavone separation method, and preparation and application of emulsifiable paste.
Background
Psoriasis (Psoriasis) is a severe non-infectious, inflammation-mediated autoimmune chronic skin disease. The pathological features of psoriasis are keratinocyte proliferation and infiltration of T cells, macrophages, dendritic cells and neutrophils. The currently accepted pathogenesis is a chronic inflammatory response with IL-23/Th17 as an axis, with myeloid dendritic cells releasing IL-23 and IL-12, activating IL-17 producing T cells, th1 cells and Th22 cells, producing abundant psoriasis cytokines IL-17, IFN-gamma, TNF and IL-22, thus inducing the clinical features of psoriasis. At the same time, these cytokines mediate the hyper-proliferation of keratinocytes and the release of chemokines, involved in the psoriasis inflammatory response. Psoriasis is easy to recur due to long disease period, and can not be healed, so that the life quality of patients is seriously influenced. Psoriasis treatment requires long-term administration, and the choice of treatment method depends on the severity of the disease. Psoriasis is often classified as mild or moderate, severe, depending on the clinical severity of the lesions (PASI score), the percentage of affected body surface area (BSA score) and the quality of life of the patient. At present, the patients with light and moderate psoriasis generally use external medicines, and patients with moderate and severe psoriasis generally need systemic treatment. The current research and development directions of psoriasis medicines at home and abroad mainly comprise biological agents such as IL-12/23p40 antibody, IL-17A antibody, TNF-alpha antibody and the like, and micromolecular medicines such as PDE4 inhibitor, JAK inhibitor, nrf2 stimulant and the like. The traditional Chinese medicine aspect is a compound preparation such as a compound natural indigo capsule, a tripterygium wilfordii multi-sweet tablet, a radix curcumae silver tablet, a silver eliminating capsule and the like. To date, specific therapeutic methods and effective drugs for the treatment of psoriasis are lacking.
Ampelopsis grossedentata is a dry whole plant of Ampelopsis grossedentata, etc. of the same genus of Vitaceae, and generally grows from a valley forest or a hillside bush, and has an altitude of 200-1500 m. The ampelopsis grossedentata (Ampelopsis grossedentata) is prepared from stems and leaves of ampelopsis grossedentata (Ampelopsis grossedentata) of Vitaceae by the processes of spreading, deactivating enzymes, twisting, naturally stacking, drying, re-drying, selecting, grading, sterilizing and the like, and maintains the inherent color, fragrance, taste and shape of ampelopsis grossedentata for brewing and drinking. The fine powder of the root and the stem is often used as vine tea powder.
Ampelopsis grossedentata is rich in active ingredients such as flavonoid compounds, phenols, steroids, terpenoids, volatile oil and the like, amino acids, vitamins, mineral elements and the like. Vine tea is sweet, light and cool in nature and has the effects of clearing heat and detoxicating, dispelling wind-damp, strengthening tendons and the like. Modern pharmacological researches have suggested that vine tea may be used in enhancing immunity, treating common cold with fever, diminishing inflammation, sore throat, treating jaundice type hepatitis, reducing blood fat and blood sugar etc. The application of the young stem leaves of the tea for treating diseases has been in the history of hundreds of years.
Because vine tea has more basic sources and similar chemical components, on the basis of a large number of folk applications and modern pharmacological researches, aiming at the application records of vine tea for enhancing immunity, resisting bacteria and diminishing inflammation, and combining the pathogenesis and treatment strategy of psoriasis, a traditional Chinese medicine preparation which adopts vine tea as a raw material and is used for preventing and/or treating psoriasis of people and a preparation method thereof are developed.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a vine tea stem or leaf total flavone separation method and a preparation method and application of emulsifiable paste.
The first aspect of the invention provides the use of a vine tea stem or leaf total flavone extract for the preparation of a medicament for the prevention and/or treatment of psoriasis.
The second aspect of the invention provides a method for extracting and separating total flavonoids from stems and leaves of vine tea.
In a third aspect, the invention provides a method for preparing a emulsifiable paste of a vine tea stem or leaf total flavone extract.
In order to achieve the above purpose, the technical scheme adopted is as follows:
a method for extracting and separating total flavonoids from stems or leaves of Ampelopsis grossedentata comprises the following steps:
step 1: pulverizing stem or leaf of Ampelopsis Grossdentata, and sieving to obtain powder;
step 2: dividing the powder into two parts, adding water into the first part of powder, heating and refluxing, and filtering while the powder is hot to obtain filtrate A; adding water into the second powder, heating and refluxing, and filtering while the second powder is hot to obtain filtrate B; combining the filtrate A and the filtrate B to obtain filtrate;
step 3: concentrating the filtrate, adding solvent, performing ultrasonic treatment, standing for separation, collecting supernatant, recovering under reduced pressure, volatilizing solvent to obtain folium Ampelopsis Grossdentata total flavone extract; or standing the filtrate in refrigerator at temperature not higher than 4deg.C, collecting the precipitate, and evaporating to remove water to obtain total flavonoids extract of Ampelopsis grossedentata.
Pulverizing stem or leaf of Ampelopsis Grossdentata in step 1 into powder for 30-60s, and sieving with 40 mesh sieve;
the mass volume ratio of the first part of powder to water in the step 2 is 1g:40-80ml, heating reflux time is 1-2h; the mass volume ratio of the second part of powder to water is 1g:16-40ml, heating reflux time is 0.5-1h;
the solvent adopted in the step 3 is ethyl acetate, and the volume ratio of the filtrate to the ethyl acetate is 1 (8-12); the ultrasonic time is 0.5-1h; the pressure of the decompression is 900-1000pa; placing the filtrate in a refrigerator at 4 ℃ for standing for 24-72h;
a vine tea stem or vine tea total flavone extract cream consists of the following components in parts by mass:
200-800mg of vine tea stem or vine tea total flavone extract
Propylene glycol I1000-2000 mg
Glyceryl monostearate 500-1000mg
Stearyl alcohol 100-300mg
200-400mg of liquid paraffin
Polysorbate 80-500 mg
Propylene glycol II 1000-1500mg
5-15mg of ethylparaben
3-6g of sterile water
The preparation method of the emulsifiable paste of the vine tea stem or vine tea total flavone extract comprises the following steps:
grinding the vine tea stem total flavone extract or vine tea total flavone extract and propylene glycol I into fine paste for standby;
heating glyceryl monostearate, stearyl alcohol and liquid paraffin in water bath to 60-80deg.C to melt to obtain oil phase;
dissolving polysorbate 80, propylene glycol II, and ethylparaben in sterile water, heating to a temperature close to the oil phase to obtain water phase, adding water phase into the oil phase under stirring, stirring for condensing, adding the fine paste when the paste is semisolid, and stirring.
The invention provides an application of vine tea stems or vine tea total flavone extract emulsifiable paste in preparing a medicament for preventing and/or treating psoriasis.
The beneficial effects of the invention are as follows:
the total flavone extract cream of the vine tea stems or leaves improves the PASI score of the mice with psoriasis after the mice with psoriasis induced by imiquimod are subjected to the intervention treatment; reducing massive infiltration of inflammatory cells in dermis; inhibiting abnormal proliferation of epidermal cells; reducing oxidative damage to the skin. Therefore, the vine tea stem or leaf total flavone extract cream can be used for preparing medicines for preventing and/or treating psoriasis, and provides a more updated and wider choice for treating psoriasis.
Drawings
FIG. 1 is a flow chart of a total flavonoids extract cream of Ampelopsis grossedentata stems or leaves for intervention in psoriatic mice;
FIG. 2 shows the effect of ampelopsis grossedentata stem or leaf total flavone extract cream on the skin of the back of psoriatic mice;
FIG. 3 is the effect of ampelopsis grossedentata stem or leaf total flavone extract cream on psoriasis mice body weight and PASI score;
FIG. 4 shows the effect of the ampelopsis grossedentata stem or leaf total flavone extract cream on spleen index and inflammatory factors in serum of psoriatic mice;
FIG. 5 is the effect of ampelopsis grossedentata stem or leaf total flavone extract cream on T-AOC, CAT, MDA in the skin of the back of psoriatic mice;
FIG. 6 shows the results of staining the skin histopathological sections of the back of psoriatic mice with the total flavonoids extract cream of Ampelopsis grossedentata stems or leaves.
Detailed Description
Materials:
imiquimod cream: purchased from Sichuan Ming Xin pharmaceutical Co.Ltd
Hematoxylin and eosin staining solution: purchased from Pinctada martensii.
Murine IL-17 detection kit: purchased from euphoria biotechnology limited.
Balb/c mice: purchased from Liaoning long Biotechnology Co., ltd.
Glycerol monostearate: the light complex technology development of Tianjin is available in the city of Tianjin.
Stearyl alcohol: the Tianjin market metallocene chemical reagent plant.
Liquid paraffin: the Tianjin city Fuyu fine chemical Co., ltd.
Ethyl hydroxy benzoate: tianjin party science and technology Co., ltd.
Propylene glycol: the chemical reagent manufacturing company of Tianjin city is constantly growing.
Polysorbate 80: the company Miou chemical reagent, inc. of Tianjin City.
The heating reflux process is carried out in a heating sleeve;
the decompression recovery process adopts a diaphragm pump to decompress;
example 1
Extraction and separation method of vine tea stem and leaf total flavonoids
1.1 extraction and separation method of total flavonoids of vine tea stems
Pulverizing Ampelopsis grossedentata stem into powder for 30s, sieving with a 40-mesh sieve, precisely weighing 2.5g of powder, placing into a round bottom flask, adding 100ml of water for the first time, heating and refluxing for 1h, and filtering while the powder is hot. 50ml of water is added for the second time, the mixture is heated and refluxed for 0.5h, filtered while the mixture is still hot, the two filtrates are combined, concentrated to 10ml, and added with 100ml of ethyl acetate for ultrasonic treatment for 1h and placed in a separating funnel. Standing, layering, collecting supernatant, recovering under reduced pressure, volatilizing, weighing to obtain 66.3mg extract, and measuring total flavone content to obtain 11.69mg extract with extraction rate of 0.46%.
1.2 extraction and separation method of total flavonoids of vine tea
Pulverizing folium Ampelopsis Grossdentata into powder for 30s, sieving with 40 mesh sieve, precisely weighing 2.5g of powder, placing into round bottom flask, adding 100ml of water for the first time, heating and refluxing for 1h, and filtering while hot. Adding 50ml of water for the second time, heating and refluxing for 0.5h, filtering while the mixture is hot, combining the two filtrates, standing for 48h in a refrigerator at 4 ℃, taking crystals, placing the crystals in an evaporation dish, volatilizing water, weighing to obtain 552.8mg of extract, and measuring the content to obtain 508.6mg of total flavone with an extraction rate of 20.34%.
Example 2
Preparation method of emulsifiable paste of vine tea stem total flavone extract
360mg of the vine tea stem total flavone extract and 1080mg of propylene glycol I are ground into fine paste for standby. Heating glyceryl monostearate 676mg, stearyl alcohol 225mg and liquid paraffin 338mg in water bath to 78deg.C to melt to obtain oil phase; dissolving 338mg of polysorbate 80, 1464mg of propylene glycol II and 10mg of ethylparaben in 5.52ml of sterile water, heating to a temperature close to that of the oil phase to obtain a water phase, adding the water phase into the oil phase under continuous stirring, stirring for condensation, adding the fine paste when the paste is semisolid, and stirring uniformly.
Example 3
Preparation method of emulsifiable paste of vine tea total flavone extract
600mg of vine tea total flavone extract and 1800mg of propylene glycol I are ground into fine paste for standby. Heating glyceryl monostearate 600mg, stearyl alcohol 200mg and liquid paraffin 300mg in water bath to 78deg.C to melt to obtain oil phase; 300mg of polysorbate 80, 1300mg of propylene glycol II and 10mg of ethylparaben are dissolved in 4.9ml of sterile water, the mixture is heated to a temperature close to the temperature of the oil phase to obtain a water phase, the water phase is added into the oil phase under continuous stirring, stirring and condensing are carried out, when the paste is semisolid, the fine paste is added, and stirring is carried out.
Example 4
4.1 animal model building and group administration
Psoriasis was induced in female Balb/c mice of 6-8 weeks of age. Mice were purchased from Liaoning Changsheng Biotechnology Co., ltd, use license: SYXK (Liao) 2021- -0009. All mice were acclimatized to the laboratory environment (20.+ -. 2 ℃, 60.+ -. 5% humidity, 12/12h light/dark cycle) for 2 weeks prior to the experiment. All animal protocols were designed according to the guidelines for laboratory animal care and use, and approved by the ethics committee of animal house, university of Shenyang pharmacy. Mice were modeled for psoriasis and on day 0 were subjected to a dehairing treatment with dehairing areas of 2cm x 4cm. Mice were divided into five groups and dehairing was performed on day 0; the 62.5mg imiquimod cream is weighed and smeared on the back dehairing areas of five groups of mice at the same time in the morning on days 1-7; blank groups were untreated every day afternoon on days 1-7; 100mg of a cream without the total flavonoids extract of vine tea stems or leaves is administered every afternoon on days 1-7 of the model group; 30mg calcipotriol cream is given every afternoon on days 1-7 of the positive drug group; 100mg of the cream of 3.6% vine tea stem or leaf total flavone extract is given every afternoon on days 1-7 of the low dose group; the high dose group was given 100mg of 7.2% of the total flavonoids extract of Ampelopsis grossedentata stems or leaves every day afternoon on days 1-7. Mice were sacrificed after anesthesia with isoflurane on day 7, flow chart 1.
4.2 mice weight and Back skin PASI score recordings
Body weight reflects the health of the mice, which are weighed daily. The PASI score reflects the severity of psoriasis in mice. Prior to sacrifice, mice were assessed daily for back skin scale and erythema severity by visual scoring from 0 to 4 by two investigators, respectively: 0, normal; 1, slightly; 2, moderately; 3, the weight; 4, extremely severe. PASI scores were calculated with a top score of 8 per animal.
4.3 Back skin pathology observations of mice
4.3.1 hematoxylin-eosin (HE) staining
The back skin was immersed in 10% formalin buffer, decalcified with 10% edta, then paraffin-embedded, and serial paraffin sections were obtained.
(1) Embedding wax blocks: after the tissue is fixed, cutting the tissue into proper size, placing the tissue into an embedding box, sequentially placing the tissue into a gradient ethanol for dehydration, rinsing the tissue with 50% ethanol, rinsing the tissue with 70% ethanol for 50min,80% ethanol for 50min,95% ethanol for I50 min,95% ethanol for II 50min, absolute ethanol for I40 min, absolute ethanol for II 40min, dimethylbenzene absolute ethanol mixed solution for 15min, dimethylbenzene for I for 10min, dimethylbenzene for II for 10min, paraffin for I1h and paraffin for II for 40min, and embedding the tissue into a paraffin block by using an embedding tray.
(2) Paraffin sections dewaxed to water: (xylene 2 cylinders for 5min each, then 100%,95%,80% gradient alcohol rehydration, 5min each), distilled water wash.
(3) And (3) dip-dyeing for 30s-1min in 0.5% solid green dyeing liquid, quickly rinsing for 2-3 times with 1% acetic acid, and washing with water.
(4) 1% safranin O solution for 3-5min (specific touch condition), 95% ethanol elution.
(5) 95% alcohol for 5min, 100% alcohol for 5min, xylene.
(6) Microscopic examination, image acquisition and analysis.
4.3.2IHC dyeing
(1) Dewaxing slices with xylene for 3 times and 15min each time, sequentially placing in absolute ethanol (2 times and 5min each time), 85% ethanol (5 min), 75% ethanol (5 min) and 50% ethanol (5 min), and washing with distilled water for 5min;
(2) If Ki67 staining is performed, the sections are immersed in a citrate antigen retrieval buffer (pH 6.0) in a retrieval cassette; if IL-23 or IL-17 staining is performed, the sections are immersed in Tris-EDTA antigen retrieval solution (pH 9.0) in the retrieval cassette. Then placing the mixture in a microwave oven, firstly starting medium fire to heat the mixture to be boiled, stopping the fire for 10min, and then continuing to heat the mixture for 10min by using medium and low fires to repair the antigen. Cooling at natural room temperature, immersing the slices in PBS (pH 7.4), and shaking and washing in a decolorizing shaker (3 times each for 5 min);
(3) The slices are immersed in a 2% hydrogen peroxide solution and incubated for 20min at room temperature in the dark to block endogenous peroxidase. Then immersing the slices in PBS (pH 7.4), and placing the slices in a decolorizing shaker for shaking and washing (3 times each for 5 min);
(4) Dripping 1% (w/v) Bovine Serum Albumin (BSA) solution in a defined range of the histochemical ring, uniformly covering tissues, and performing serum blocking at room temperature for 60min;
(5) Gently wiping off the residual sealing liquid on the slice by using filter paper, then dripping the primary antibody diluted according to a certain proportion, and placing the slice in a wet box at 4 ℃ overnight;
(6) Immersing the slice in PBS (pH 7.4), shaking and washing in a decolorizing shaker (3 times for 5min each time), sucking with filter paper, dripping diluted secondary antibody (HRP label) to cover tissue, and incubating at room temperature for 60min;
(7) Immersing the slice in PBS (pH 7.4), shaking and washing in a decolorizing shaker (3 times for 5min each time), sucking with filter paper, continuously dripping DAB color development liquid, continuously observing with a microscope to control color development time, positively appearing brownish yellow, and washing with tap water to terminate color development;
(8) The slice is counterstained with hematoxylin dye solution for 3 to 5 minutes and then washed with running water for several seconds, then differentiated with differentiation solution for several seconds and then washed with running water for 15 seconds, then blued with bluing solution and then washed with running water for 10 minutes;
(9) Sequentially dehydrating and transparency the slices in 75% ethanol (5 min), 85% ethanol (5 min), anhydrous ethanol (2 times, 5min each time), n-butanol (5 min) and xylene (5 min), air drying, and sealing with neutral resin.
(10) Microscopic examination, image acquisition and analysis.
Example 5
Effect of Ampelopsis grossedentata Stem or leaf Total flavone extract cream on psoriasis mice weight, back skin and PASI score
The weight change of mice is an important index for evaluating the toxicity of the total flavone extract cream of the vine tea stems or leaves; PASI score is an important indicator for assessing the severity of psoriasis in mice. The body weight and PASI score of the mice were measured and recorded daily on days 1-7 in this example, and the results are shown in fig. 2-3.
As can be seen from fig. 2, the back of the mice in the model group and the positive drug group showed erythema on day 3, and the erythema symptoms in the model group and the positive drug group were aggravated on day 5, while scales were generated; the low-dose and high-dose administration groups have slight scale generation, and the scale thickening of the model group and the positive medicament group is serious and even falls off on the 7 th day; both the low and high dose groups were given slight scaling to the back skin. The treatment group showed a significant improvement in back skin symptoms compared to the model group, the positive drug group, and the PASI score was consistent with morphological observations (fig. 3). It can be seen that the ampelopsis grossedentata stem or leaf total flavone extract cream can improve psoriasis mice symptoms.
Example 6
Effect of Ampelopsis grossedentata Stem or leaf Total Flavonoids extract Crispi cream on spleen index of psoriasis mice and on cytokines in vivo serum
Spleen is an important immune organ of a mouse, and spleen index can reflect the strength of the immune function of an organism to a certain extent. Psoriasis mice sacrifice harvested spleens on the seventh day of the experiment and spleen index is calculated. As can be seen from fig. 4, the ampelopsis grossedentata stem or leaf total flavone extract cream significantly increases the spleen index of mice. The psoriatic mice were subjected to orbital venous plexus blood collection, left standing for 3 hours, centrifuged, and the supernatant was subjected to ELISA to detect the serum inflammatory factor IL-17 level of the mice, and the results are shown in FIG. 4.
Example 7
Effect of Ampelopsis grossedentata Stem or leaf Total flavone extract cream on oxidative stress injury of skin on back of psoriasis mice
T-AOC is used as an important index for reflecting the total antioxidant capacity of organisms, and can be used for evaluating the protection effect of the ampelopsis grossedentata total flavone extract cream on oxidative stress injury of organisms induced by imiquimod; CAT is an important antioxidant enzyme in the organism and has the main functions of scavenging ROS and resisting oxidative stress. MDA, as a product of lipid peroxides, reflects the intensity and extent of free radical attack by the body. Imiquimod induces the occurrence of psoriasis, which results in a disruption of the balance of enzymes and oxidation products described above, and thus oxidative stress damage. As can be seen from fig. 5, the total flavonoids extract cream of the stems or leaves of the tenuifolia can remarkably improve the total antioxidant capacity and CAT activity of mice after imiquimod induction, reduce the generation of oxidation intermediate product MDA and protect the mice from oxidative stress injury.
Example 8
Effect of Ampelopsis grossedentata Stem or leaf Total Flavonoids extract cream on psoriasis mouse histopathology
Balb/c mice developed a pronounced psoriasis pattern after 7 days of imiquimod molding. To further verify the therapeutic effect of the ampelopsis grossedentata stem or leaf total flavone extract cream on psoriatic mice, paraffin sections were performed on the back skin of the mice after fixation with 4% formalin solution, and the back skin HE and IHC staining of the mice were performed, respectively, and pathological changes of the back skin of the mice were observed, and the results are shown in fig. 6. It can be seen from the figure that the skin thickness is significantly reduced; the level of inflammatory factors IL-17 and IL-23 is obviously reduced; basal layer Ki67 expression was significantly reduced.
Claims (8)
1. The method for separating total flavonoids from stems or leaves of vine tea is characterized by comprising the following steps of:
step 1: pulverizing stem or leaf of Ampelopsis Grossdentata, and sieving to obtain powder;
step 2: dividing the powder into two parts, adding water into the first part of powder, heating and refluxing, and filtering while the powder is hot to obtain filtrate A; adding water into the second powder, heating and refluxing, and filtering while the second powder is hot to obtain filtrate B; combining the filtrate A and the filtrate B to obtain filtrate;
step 3: concentrating the filtrate, adding solvent, performing ultrasonic treatment, standing for separation, collecting supernatant, recovering under reduced pressure, volatilizing solvent to obtain folium Ampelopsis Grossdentata total flavone extract; or standing the filtrate in refrigerator at temperature not higher than 4deg.C, collecting the precipitate, and evaporating to remove water to obtain total flavonoids extract of Ampelopsis grossedentata.
2. The method for separating total flavonoids from stems or leaves of Ampelopsis grossedentata according to claim 1, wherein the steps 1 are carried out by pulverizing stems or leaves of Ampelopsis grossedentata into powder for 30-60s, and sieving with 40 mesh sieve.
3. The vine tea stem or leaf total flavonoids separation method according to claim 1, wherein the mass-to-volume ratio of the first part of powder to water in step 2 is 1g:40-80ml, heating reflux time is 1-2h; the mass volume ratio of the second part of powder to water is 1g:16-40ml, and heating reflux time is 0.5-1h.
4. The method for separating total flavonoids from stems or leaves of vine tea according to claim 1, wherein the solvent in the step 3 is ethyl acetate, and the volume ratio of the filtrate to the ethyl acetate is 1: (8-12); the ultrasonic time is 0.5-1h; the pressure of the decompression is 900-1000pa; and placing the filtrate in a refrigerator with the temperature not higher than 4 ℃ for standing for 24-72h.
5. A emulsifiable paste of a total flavonoids extract of vine tea stems or leaves obtained by the method for separating total flavonoids of vine tea stems or leaves according to any one of claims 1 to 4, which is characterized by comprising the following components in parts by mass:
200-800mg of vine tea stem or vine tea total flavone extract, 1000-2000mg of propylene glycol I, 500-1000mg of glyceryl monostearate, 100-300mg of stearyl alcohol, 200-400mg of liquid paraffin, 200-500mg of polysorbate, 1000-1500mg of propylene glycol II, 5-15mg of ethylparaben and 3-6g of sterile water.
6. A method for preparing a emulsifiable paste of the tenuifolia stem or leaf total flavonoid extract as set forth in claim 5, which comprises the steps of:
grinding the vine tea stem total flavone extract or vine tea total flavone extract and propylene glycol I into fine paste for standby;
heating glyceryl monostearate, stearyl alcohol and liquid paraffin in water bath to obtain oil phase;
dissolving polysorbate 80, propylene glycol II, and ethylparaben in sterile water, heating to obtain water phase, adding water phase into oil phase under stirring, stirring for condensation, adding the above fine paste when the paste is semisolid, and stirring to obtain the cream of Ampelopsis grossedentata stem or Ampelopsis grossedentata total flavone extract.
7. The method for preparing a cream of total flavonoids of vine tea stems or leaves according to claim 6, wherein the heating temperature is 60-80 ℃.
8. Use of a cream of total flavonoids of Ampelopsis grossedentata stems or Ampelopsis grossedentata in preparing medicines for preventing and/or treating psoriasis is provided.
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