CN106727943A - One kind treats phthisical pharmaceutical composition and preparation method thereof - Google Patents
One kind treats phthisical pharmaceutical composition and preparation method thereof Download PDFInfo
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Abstract
Phthisical pharmaceutical composition and preparation method thereof is treated the invention discloses one kind, pharmaceutical composition of the present invention is with colour of loess a kind of reed mentioned in ancient books, Holothuria atra, Fructus Corni, kelp base, removes Methscopolamine, tin dilaurate zinc, lithium oleate as bulk drug, proportioning is formed, routinely various formulations can be made by preparation process, treatment pulmonary tuberculosis is evident in efficacy.
Description
Technical field
Phthisical pharmaceutical composition and its preparation side are treated the invention belongs to technical field of traditional Chinese medicines, more particularly to one kind
Method.
Background technology
Pulmonary tuberculosis is one of common communicable disease.In recent years, the phthisical incidence of disease of China is raised year by year, and young
Change trend is obvious ....Clinically, for tubercle bacillus conventional sterilization, antibacterial medicines use in conjunction scheme are achieved and are more satisfied with
Therapeutic effect, but with the extension of administration time, Mycobacterium tuberculosis drug-resistant problem is on the rise.The development of the medicine is treatment lung
Tuberculosis provides reliable direction, and can significantly improve therapeutic effect, drug safety, clinic popularization and application.
Colour of loess a kind of reed mentioned in ancient books：Spend the root of the root of large-flowered skullcap partially for labiate.Summer, autumn excavation, clean, and dry.【Original shape state】Huang is spent partially
A kind of reed mentioned in ancient books, perennial herb.Stem is upright or rises, and has creeping rootstock sometimes, even inflorescence 8-30cm high, thick 1.2-2.5mm, circle prismatic,
Pubescence long is saved by white tool, base portion is how many lignifying.Generally only 3-4 pairs, leaf, at the beginning as rosette-stape is arranged, later due to internode
Elongation is in interaction opposite, and middle leaf is maximum；Petiole 1-5cm long, middle part person is most long, flat, close to save pubescence long by white tool；Blade
Hard papery, ovate-elliptic oval or wide, 4.5-5.5cm long, 3.8-4.5cm wide, inferior leads and nearly inflorescence leaf diminish, row end
Circular or blunt, base portion is heart-shaped or circular, and edge has shallow wavy knuckle-tooth, above green, below it is thin, have orange gland point, two sides is equal
Strigose is saved by white tool, especially with following upper for very, lateral vein about 4 pairs is not significant above, protrudes below along the pulse, the side in leaf margin
Net knot.Flower opposite, in the raceme that dorsal ventral side is arranged on stem top, 7-15cm long, scape shape, rachis is soft by tool section length
Hair, common peduncle 1-4.5cm long；Bennet is about 2mm, there is tool section pubescence long；Bract is small, oval, tapered to inflorescence top, is about
2.5mm, it is close by pubescence；Pale purple to the cruel blueness of corolla, 1.8-2.5cm long, base portion is gradually wide upwards into shape of bending knee, and throat is wide to be reached
Micro- pubescence is dredged in 6mm, outside, and, by micro- pubescence, remaining part is without hair, the lip of limb two, upper lip helmet for inner face only lower lip and corolla tube middle part rear
Shape, tip nick, sliver is semicircle in lower lip, two side sliver ovals；Stamen 4, the last two；Filigree is elongated, flat, below middle part
By cilium；Floral disc is loose thick, inclines, and front is swelled on tiltedly；Ovary handle is more long can not very substantially, and style is elongated, without hair, ovary light
It is sliding.The month at florescence 3-5.【Nature and flavor】Bitter；It is cold in nature.【Return through】Lung；Large intestine channel.【Indication】Removing heat from the lung to relieve cough；Dampness-eliminating and dysentery-stopping.Main lung
Heat cough；Spitting of blood is damp and hot；Dysentery.【Take passages】《Chinese book on Chinese herbal medicine》.
Holothuria atra：It is the entirety of Holothuriidae animal Holothuria atra, black red star sea cucumber, Michaelis sea cucumber and phase sibling species.Diver
Lower water is fished for many in spring, autumn, it is also possible to trawl fishing, but big to the destruction Of resources, disables more.Internal organ are removed after catching, chamber is cleaned
Interior silt, blood stains, boil about 1h in salt solution, pick up and let cool.Through exposure or bakee to 8 ninety percent it is dry when, then in Gabon's leaf juice slightly
Person, takes out when turning black to color and dries.【Original shape state】1. Holothuria atra, the general a length of 20-30cm of body, old larger individuality is reachable
45-60cm, the inclined outside of belly of mouth has tentacle 20.Body back of the body parapodum is small and lack, and outside of belly pipe is small and dense enough, equal irregular arrangement.Dermal bone
Piece：Broadcasting TV programs by satellite, table chassis is small and undeveloped, and periphery is circularized, and tower top has 12 small teeth, and peripheral 8 levels are separated, and middle 4
Individual setting.Another osteocomma is in simple rosette.2. black red star sea cucumber, general body 20cm long or so, there are most rows at the back side
Irregular parapodum is arranged, outside of belly pipe foot close, the inclined outside of belly of mouth more has tentacle 20, and its upper end is slightly in dendroid when trailing.Skin
Endosclerite has table shape and rhabodoid.3. Michaelis sea cucumber, general body 10-15cm long, the scattered a small number of small warts in the back side, outside of belly pipe foot is flourishing
And it is close.The mouth outside of belly, tentacle 20 partially, during stretching, extension, there is branch its top and lower section and is in dendroid.Intracutaneous osteocomma is mainly summary
The rhabodoid of bending, separately there is special " H " shape osteocomma.【Take passages】《Chinese book on Chinese herbal medicine》.
Fructus Corni：It is the fruit of Cornaceae plant Fructus Corni.Harvesting and storage：Nursery need to cultivate 6-7 to result,
15-20 is the best fruiting period.9-11 the first tenday period of a month month fruit produce in it is red with when it is ripe, pluck in batches, never damage bud.Processing method can use
Water-boiling method；Min in boiling water is put with new fresh fruit by red, boshing water is pulled out in time, seed is extruded while hot, pulp is dried or dried i.e.
Into.Also mechanical threshing method can be used, extrusion pulp is dried.
【Proterties】Meat pericarp ruptures shrinkage, imperfect or in flat drum shape, is about 1.5cm, about 0.5cm wide.New goods surface
It is aubergine, there is a circular place calyx vestige on old to see to be generally atropurpureus long, glossy, base portion visible carpopodium trace sometimes, top.Matter
It is soft and moist non-friable.Odorless, sour and puckery hardship.With seedless, skin and flesh it is plump, chilli oil profit person is good for color.【Chemical composition】Fructus Corni pulp
Containing tannin compositions：Fructus Corni tan hair（Cornus-tannin) 1,2,3, Fructus Corni tannin 1 is different terchebin
（) also known as water chestnut belongs to tannin isoterchebin（Trapain) [4], Fructus Corni tannin 2 is new miterwort element II（tel-
Limagrandin II) [2], Fructus Corni tannin 2 is new miterwort element I（tellima-grandinⅠ）；（Wood comes）Wooden tannin
（Cornusiin) A, B, C, [2], G [3], clove tree tannin（Eugeniin), yellow avens tannin D（) and 2,3- bis- geminD-
O- galloyl glucoses（2,3-di-O-glloy- β-D-glucose), 1,2,3- tri--O- galloyl glucoses（1,2,6-
Tri-O-galloyl- β-D-glucose), 1,2,6-dg-O-D-glucose), 1,2,6- tri--O- galloyl glucoses
（1、2、6-tetro-O-galloyl-β-D-glucose).And glucoside-containing composition；Fructus Corni splits glucoside（Cornuside), Mo Luoren
Winter glucoside（), morroniside 7-O- methyl morroniside（7-O-methylmorroniside), vomiting nut glucoside
（Loganin), sweroside（sweroside).Also contain glucose（Glucose), fructose（Fructose), sucrose（su-
Crose), ursolic acid（Ursolic acid), gallic acid（Gallic acid), malic acid（Malic acid), tartaric acid
（Tartaric acid) and vitamin A（Vitamin A) etc..Again containing hair oil is given as security, therefrom isolated 9 monoterpene hydrocarbon, 6 times
Hemiterpene hydrocarbon, 5 aliphatic hydrocarbons, 7 monoterpene esters and a fatty alcohol, 4 monoterpene aldehyde and ketone, 3 fatty aldehydes and ketone, 4 acid, 18 esters
With 15 aromatic compounds, the wherein more main component of content has：Isobutanol（Isobutyl alcohol), butanol
（Butanol), isoamyl alcohol（Isoanyl alcohol), cis and trans camphor tree alcohol oxide of premeditating（linalool ）, chaff
Aldehyde（Furfural), β-benzene alcohol（β-phenyl ethylalcohol), methyleugenol（Methyl eugenol), olive
Face cream element（Elemicin), different asarum bridal veil（Isoasarone), palmitic acid ester（Ethylpalmitate), oleic acid ester
（Ethyloleate), linoleic acid ester（Ethyllinoleate), benzyl cinnamate（Benzyl cinnamate), palmitic acid
（Palmitic acid), stearic acid（Stearic acid),（King is ancient）（Wang Ba）Alkene（Copaene), alpha-terpineol（α-
Terpi-neol), α-curcumene（α curcumene), anethole（Anethole), dioxane between 4- methoxyl group -1,2- benzos
Amylene（4-methoxy-1,2-benzodioxole), asarone（Asaricin), aristolone（Aristolone), basic note
Oxalaldehyde（Ethylvanillin), leukotrienes ester（Ethyllinolenate), pulegone（Pulegone), safrole
（Safrole) etc..Contain linoleic acid in core（Linoleic acid), oleic acid（Oleic acid), palmitic acid（palmitic
Acid), stearic acid（Stearic acid), leukotrienes（Linolenic acid), laurate（Lauric acid) etc. aliphatic acid
With 21 kinds of elements [9] such as iron, aluminium, copper, zinc, boron, phosphorus.Contain threonine in pulp and core（Threonine), valine
（Valine), leucine（Leucine), isoleucine（Isoleucine), phenylalanine（Phenylalanine), histidine
（Histidine), lysine（Lysine), serine（Serine), glutamic acid（Glutam-ic acid), glycine
（Glycine), alanine（Alanine), tyrosine（Tyrosine), arginine（Arginine), aspartic acid（aspartic
) etc. acid 14 kinds of amino acid, also separately there is methionine in core（Methionine), proline（Proline), cystine
（cystine).【Pharmacological action】1. antibacterial action fruit decoction can suppress the growth of staphylococcus aureus in vitro, and right
Escherichia coli are then invalid.Decoction（1:1）To the inhibition zone of Shigella shigae, diameter is up to 13-18mm（Flat board ring agar diffusion method）.
A dark red tart flavour liquid can be obtained from fresh dogwood fruits meat, has inhibitory action to typhoid fever, dysentery bacterium.Flooding agent（1:3）
There is different degrees of inhibitory action to trichophyton in test tube.2. the influence of the normal rat blood sugar of hypoglycemic effect 2.1 pairs
Outbreeding system Wistar male white rats, body weight 150-200g, 36 are divided equally 4 groups.1 group is control group, and remaining 3 groups is medicine group,
Difference 5,7.5,15g/（kg,d）Medicine（Fructus Corni mature sarcocarp.Extracted with 70% alcohol reflux after fine ground, medicinal extract 1ml processed（Phase
When in 3.5g crude drugs）ig.Medicine group and control group blood glucose value no significant difference.It is another once with 50g/kg medicines to rat ig1 week, tie
Fruit also has no and normal rat blood sugar value is had a significant effect.2.2 pairs of influence rats of adrenal gland disposition Hyperglycemia In Stz-induced Diabetic Rats 24,
Respectively 3 groups, after fasting 2 hours, Normal group and moulding control group ig feed water, and administration group ig gives Fructus Corni 7g/kg.After administration
1 hour, moulding control rats sc physiological saline；Moulding control group and administration group rat sc adrenal gland 0.25mg/kg.After injection
135 minutes measuring blood sugar of blood extracting.It can be seen that Fructus Corni plays the role of significantly to antiadrenergic drug hyperglycaemia.2.3 pairs of four oxygen are phonetic
The influence rat of pyridine Hyperglycemia In Stz-induced Diabetic Rats, hepatic glycogen, triglycerides and cholesterol 24, respectively 3 groups.1 group is Normal group,
The another 2 groups alloxan normal saline solutions in iv30mg/kg under Rat Tongue, the daily ig Fructus Cornis 7g/kg of one of which.Experiment
Result shows that Fructus Corni has a significant effect to the hyperglycaemia of alloxan diabetes rat（P ＜ 0.05）, there is certain rising
The effect of rat hepatic glycogen, but triglycerides and cholesterol are had no significant effect.2.4 pairs of shadows of prontosil rat diabetes
Ring using Wistar systems male white rat.Animal stop eating 45 hours after by 50-65mg/kgiv prontosil induced Diabetics.Will
Rehmanniae Bolus of Eight Ingredients is made corresponding extracta sicca and pulvis, and other components are then made pulvis.Fructus Corni is removed and is made extracted by ether agent
（COE）And it is further separated out other 3 subfractions：COEfr-1, COE mono- fr-2, COE-rf-3.Then, then from COE-
Three kinds of one fish jecoric acid of crystallization oil, malol and 2-a- hydroxyl malols are obtained in fr-2, first two is used for experiment, Fructus Corni and eight
The pulvis of taste Dihuang Wan is 2g/kg, and 3 subfractions and morrhuic acid and malol of COE1g/kg, COE are respectively 0.5g/kg.
The method of administration is：Animals iv injects prontosil（Streptozotocin）Afterwards lh start po administration, daily 2 times later,
It is administered 6 times altogether, blood sugar detection is carried out from the big venous blood sampling in abdominal cavity when 3 hours after being administered in last 1 time.Simultaneously determine glucose in urine and
The animal amount of drinking water of 20 hours and urine volume.Test result indicate that, with the pulvis of Rehmanniae Bolus of Eight Ingredients and the anti-glycosuria of Fructus Corni therein
The effect of disease is the most obvious, and after Fructus Corni powder delivery, the animal amount of drinking water of 20 hours is 23.3 ± 2.8, with control group（46.4
±50）Compare, difference is very significant（P ＜ 0.0001=.Blood sugar（mg/dl）And glucose in urine content（Urinate within mg/20 hours）Respectively
393.3 ± 28.7 and 2553.0 ± 205.1 compared with control group（470.0 ± 21.3 and 4733.7 ± 389.5）With substantially poor
The former P ＜ 0.05, the latter P ＜ 0.0001=different.The effect of the hypoglycemic and glucose in urine of Rehmanniae Bolus of Eight Ingredients pulvis is also non-to learn obvious, and
Its dry extract is then without positive effect.In the extracted by ether agent of Fructus Corni, the activity of especially COE-fr-2 is most strong.COE-fr-2 gives
After medicine, the animal amount of drinking water of 20 hours and urine volume are respectively 13.8 ± 3.4 and 13.5 ± 1.9, compared with control group（50.0±
3.2 and 36.7 ± 30）Difference is very significant（P ＜ 0.001=, blood sugar and glucose in urine content are respectively 227.2 ± 25.3 Hes
978.2 ± 1678 compared with control group（403.3 ± 36.6 and 2916.8 ± 55.4）, it is respectively provided with significant difference（The former is P ＜
0.05, the latter P ＜ 0.0001=, in addition, COE and COE-fr-l also have obvious urine glucose lowering to act on, and COE and COE-fr-2 have
Reduce the trend of the weight of animals.To separating obtained fish jecoric acid oil and malol from COE-fr-2, although both of which can dramatically
Ground reduces the animal amount of drinking water of 20 hours and urine volume, but only malol can significantly reduce blood sugar and glucose in urine.Malol is given
After medicine, the blood sugar and glucose in urine content of animal are respectively 294.2 ± 37.2 and 1175.5 ± 249.2, compared with control group（513.2
± 35.4 and 3013.3 ± 404.2）With very significant difference（P ＜ 0.0001）The 2.5 pairs of hyperglycemic rat WBVs
Influence alloxan diabetes rat ig Fructus Cornis after 7g/kg1 weeks, tail vein measuring blood sugar of blood extracting.40mg/kg penta is used again
Barbital sodium solution is anaesthetized, anticoagulant heparin, abdominal aortic blood, determines WBV.2.6 pairs of hyperglycemic rat platelet aggregations
Influence Fructus Corni 7h/kg, 2 hours after last ig alloxan diabetes rats, with 3.8% sodium citrate 9：L ratios resist
Solidifying, abdominal aortic blood prepares rich platelet and platelet poor plasma, is to cause poly- agent with ADP, determines medicine to platelet aggregation
The influence of function.As a result it is 44.6% that the blood platelet of Fructus Corni group suppresses PAR（Effects of the P ＜ 0.00013. to immune system
3.1 pairs of influence male ICR mouses of immune organ weight 40, are randomly divided into 4 groups, the continuous 5dig Fructus Cornis of experimental group
10.20g/kg（Water decoction）；Control group gives same amount water daily, positive controls in d3, continuous 3dip endoxan
50mg/kg.24h after last dose, sacrificed by exsanguination animal is won mouse thymus, spleen and liver and claims the calculating of its weight in wet base each dirty
The index of device.The thymus index of Fructus Corni reduction mouse, it is not significant on liver, spleen influence.3.2 pairs of carbon grains clean up speed
Influence takes male ICR mouse, and the dosage and mode of Fructus Corni and endoxan are ibid.24h after last dose, by document side
Method calculates mouse and cleans up index（K）And index is cleaned up in correction（a）.As a result the Fructus Corni group K values of various dose are significantly lower than
Control group.The influence male NIH mouse of the 3.3 couples of influence 3.3.1 of mice serum antibody to hemolytic antibody, administration group is in silk floss
Sheep red blood cell（SRBC）2d begins before sensitization, continuous 5dig Fructus Cornis 5.10g/kg, positive controls ip endoxan 25mg/
Kg, every time 24h after administration, by colorimetric method for determining and calculate the HC50 of sample.From table 6, Fructus Corni 10g/kg is significantly raised
Mice serum hemolytic antibody content, Fructus Corni 5g/kg effects are not significant.3.3.2 the influence BALB/C of antagonist IgG content
Mouse, male and female dual-purpose, the dosage of Fructus Corni ibid, positive controls in experimental group administration d4, continuous 2dip rings phosphorus
Acid amides 100mg/kg.24h after last dose, mouse orbit blood sampling, separates serum, and IgG content is surveyed by agar single radial immunodiffusion,
IgG content, is represented with deposit ring diameter.The even energy of various dose Fructus Corni is significant to raise mice serum IgG content.3.4 couples of SRBC
The influence of caused delayed footpad reaction takes male mice in kunming, experimental group is administered in 1d before SRBC sensitization, and Fructus Corni is given
, with 3, lh antigens are attacked after last dose for pharmaceutical quantities and mode, the thickness difference and weight difference of mouse or so foot pad are surveyed after 24h, as a result
Fructus Corni 5,10g/kg suppresses SRBC induced mice Delayed footpad swellings.The influence of contact dermatitis caused by 3.5 couples of DNCB
3.5.1 influence of the administration to contact dermatitis takes female NIH mouse before attacking, experimental group in d6 after DNCB sensitization, continuously
5dig Fructus Cornis 10,20d/kg, 6h after last dose is applied to mouse right ear and is attacked with DNCB, and two ears are cut after 16h, with
Diameter 9mm card punch lays auricle, and the degree of contact dermatitis is represented with the weightening of auris dextra, and as a result various dose Fructus Corni is to small
The auricle edema of mouse shows obvious inhibitory action.3.5.2 the influence male NIH being administered to contact dermatitis after attacking is small
Mouse 56, is randomly divided into 7 groups, the same document of experimental technique, in dl0 after DNCB sensitization, administration group different time after antigen is attacked
Ig Fructus Cornis 20g/kg.Fructus Corni can substantially suppress DNCB induced mice contact dermatitis in administration in 3-15 hours after attack.
Externally applied drug in Cornus officinalis plantations body, immunosuppressive action is to mouse；Ursolic acid in vitro test can kill culture
Mouse lymphocyte, make to lose pouring and turn, the ability that IL-2 generations and LAK cells are produced, but such as intraperitoneal injection ursolic acid, to small
The These parameters of mouse are significantly improved.4. influence of the Antishock function 4.1 to Blood pressure of carotid artery takes 1.5-3.5kg healthy rabbits,
Male and female are regardless of, and are randomly divided into two groups, and first group is experimental group, and second group is control group.In the descending arteria carotis stripping of 2% procaine local anaesthesia
From intubation connects Rm-600 types four and leads physiograph, ear vein injection 312u/（M1, kg）Heparin.Then start femoral artery to delay
Slow play blood, makes blood pressure drop to below 50mmHg.Due to the compensation of rabbit, blood pressure can automatic re-ascending after stopping bloodletting.If returning
When rising higher, then bloodletting again makes blood pressure maintain below 60mmHg, continues lh, and record shock stage items observation index number
It is worth, then first group gives 1g/ml Cornus officinalis injection neck subcutaneous superficial vein drops, injection speed is 36 drops/min；Second group to etc.
Amount saline i drop.Cornus officinalis injection pH is 7.68, and physiological saline pH is 7.5.Sample is taken after 30 minutes again, and is remembered
Every observation index numerical value, terminates observation after record treatment.Experimental result finds：First group by shock stage average 49.73 ±
7.25mmHg rises to 87.64 ± 7.13mmHg, and ascensional range is 39.43 ± 9.97mmHg；Second group is by shock stage average 55.46
± 8.57mmHg rises to 68.61 ± 17.6mmHg, and ascensional range is 13.15 ± 12.12mmHg.Two groups have significant difference, t=
61.8, P ＜ 0.001.4.2 pairs of first groups of the above-mentioned experiment of influence of blood pressure heartbeat wave-amplitude are by shock stage average 1.42 ± 0.7mm
4.03 ± 1.11mm is increased to, increase amplitude is 2.61 ± 0.72mm；Second group is increased to by the body average 1.88 ± 0.9mm that sets a time limit
2.35 ± 0.87mm, increase amplitude is 0.57 ± 0.93mm.Two groups have significant difference, t=5.36, P ＜ 0.001.5. inflammation is suppressed
Reagentia 5.1 suppresses mouse peritoneal capillary permeability male mice in kunming, body weight 18-22g.Positive controls are daily
Ig aspirin 50mg/kg；The daily ig Fructus Cornis 5,10g/kg of administration group（Water decoction 1g/ml containing crude drug）, continuous 5d；Blank pair
According to the daily water for giving equivalent of group, lh after last dose, each rat-tail iv0.5%Evans indigo plants 5ml/kg, ip0.7% acetic acid after 5min
10ml/kg, by mouse sacrificed by exsanguination after the 30min of interval, abdominal cavity is repeatedly rinsed with distilled water, is collected flushing liquor and is diluted to 10ml
Lh is placed, colorimetric is located with 751 type spectrophotometer 610nm, it is as a result right by the standard curve dyestuff seepage discharge of the blue absorptions of Evans
According to group, aspirin group, Fructus Corni 5.10g/kg group dyestuff seepage discharges（ug/ml）Respectively 91 ± 1.6,4.9 ± 2.5（P ＜
0.01）, 5.3 ± 2.0（P ＜ 0.01）, 5.5 ± 2.8（P ＜ 0.01）（X ± S, n=10）.It can be seen that various dose Fructus Corni Dichlorodiphenyl Acetate
The rat abdominal cavity capillary permeability increase for causing has obvious inhibiting effect.5.2 suppress dimethylbenzene induced mice swelling of auricle
Swollen male mice in kunming, body weight 18-22g, administration group continuous 5dig Fructus Cornis 10.20g/kg, 1h after last dose, by diformazan
Benzene 30ul is applied to mouse right ear exterior feature, and left auricle compares, auricle is cut after 2 hours and auricle is removed with diameter 9mm card punch, claims
Weight.As a result control group, Fructus Corni 10.20g/kg group mouse or so auricle weight difference（mg）Respectively 18.2 ± 4.5,99 ±
4.5（P ＜ 0.01）, 11.3 ± 4.3（P ＜ 001）,（N=10）.Show that Fructus Corni is significant and suppress dimethylbenzene induced mice auricle
Swelling.The rat footpad swelling male SD rat that 5.3 suppression proinflammatory agents cause, body weight 120-160g, positive controls, administration
The continuous 5dig aspirin 50mg/kg and Fructus Corni 5g/kg of group difference, l hours after last dose, in foot pad scl% after Rat Right
Carrageenan measures a footpad swelling degree with caliper-gauge per hour into fresh albumen 0.1ml after causing inflammation（mm）, as a result Fructus Corni
5g/kg has obvious inhibiting effect to swelling caused by egg white, and footpad swelling caused by Carrageenan suppresses to make only at 1 hour
With significant.5.4 suppress rat granuloma hyperblastosis male Wistar rat, body weight 150-220g, etherization, in two
Each heeling-in 20mg weights sterilized cotton ball one under the forelimb armpit of side, positive controls, administration group distinguish continuous sc hydrocortisones
10mg/kg and ig Fructus Cornis 5,10g/kg, rat peels off granulation tissue in d8 sacrificed by exsanguination, and 80 DEG C of bakings are weighed after 3 hours,
And take each mouse thymus gland, adrenal gland simultaneously and weigh.Rat granuloma hyperblastosis is substantially suppressed by the visible Fructus Corni of table 12, to chest
Gland and adrenal gland weight are without significant impact.Ascorbic acid content takes granuloma experiment rat used in 5.5 reduction adult rat adrenal tissues
Right side adrenal gland, is made after being homogenized by 2,4 1 dinitrophenylhydrazine determination of color wherein ascorbic acid content.Fructus Corni 5,10g/
Kg makes the ascorbic acid content in adult rat adrenal tissue substantially reduce.The influence male of PGE contents in 5.6 pairs of rat inflammation tissues
SD rats, body weight 150-180g.Administration group, positive controls distinguish continuous 5dig Fructus Cornis 10g/kg or indocin 50mg/kg,
30 minutes after last dose, in foot pad scl% carrageenans 0.1ml behind each mouse right side, put to death after 3 hours, by right metapedes from ankle-joint
Cut at upper lcm and weighed, be placed in after peeling in 5ml physiological saline and soak lh, taken supernatant 0.15ml and add 0.5mol/L hydrogen-oxygens
Change potassium methanol solution 1ml, in 50 DEG C of water-bath isomerization 20min, plus methyl alcohol 2.5ml, ultraviolet absorption value is determined at 278nm（△A）,
PGE contents are represented with every gram of suitable absorption value of inflammatory tissue, as a result Fructus Corni 10g/kg causes scorching lumping weight amount and its PGE to rat
Content has no significant effect.6. antitumaous effect takes the small white mouse inoculation ascites cells liquid 0.1ml of health.Cancer is grown after 7-8d
Liquid is more, extracts cancer liquid out and adds one and drop on sheet glass, and Jia one 20% Fructus Corni decoction of drop and drop Yihong Tai Shi solution, is showing
The death condition of cancer cell is seen under micro mirror, as being killed, cell dyes red, dead is still then colorless and transparent.Control is just
Normal cell is salivary gland cell and spermary cell.Test result indicate that, Fructus Corni can kill whole cancer cells；Spermary cell is also
There is same purpose, but only have fraction to salivary gland cell and be killed.7. other effects once reported that liquid extract was to anesthesia in one's early years
Dog has a diuresis, and can reduce blood pressure, on normal Rabbits ' Blood Sugar without influence.Above-mentioned Acidic Liquid, to the frog, mouse, rat, rabbit
Toxicity less, on body weight and blood picture without influence, rabbit gastric mucosa mild hyperaemia can be caused, but to rabbit conjunctiva without effect.Cornin
Toxicity is very low, not haemolysis, but has weaker excited parasympathetic effect, this product tool antioxidation.【Nature and flavor】It is sour；
Slightly warm in nature.【Return through】Return liver；Kidney channel
【Indication】Tonify the liver and kidney；Convergence is solid de-.Main waist is had a dizzy spell；Deafness and tinnitus；Soreness and weakness of waist and knees；Xian essence involuntary emission；Urine
Frequency；Incessant sweating due to debility；Woman uterine bleeding.【Take passages】《Chinese book on Chinese herbal medicine》.
Kelp base：It is the holdfast of Laminariaceae plant kelp (sea-tangle).Harvesting and storage：Summer, season in autumn two harvest sea-tangle, plus
Man-hour cuts the root base of a fruit, dries.【Original shape state】1. kelp frond olive-drab, is crineous after doing.Keratin is in banding after maturation,
General 2-6m long, 20-50cm wide, have two shallow ridges of parallel longitudinal walk in central vane, and thicker part is " middle band in the middle of two ditches
Portion ", thickness about 2-5mm, both sides of the edge are gradually thin, and have wavy gauffer, blade base wedge shape, and thick is then oblateness into the stage, under
There is a cylindrical or oblate short handle, 5-15cm long, handle and blade interior are constituted by marrow, cortex and epidermis.Outside
Have mucilage cavity in cortex, there is secretory cell in chamber, can secreting mucus to leaf body surface face, constitute gelatinous layer, make frond stick-slip and rise
Protective effect.Marrow is made up of many algal filaments, and algal filament cell one end is expanded in loudspeaker tubulose.Frond juvenile stage blade face is smooth, small
There is concavo-convex phenomenon in sea-tangle phase blade.Annual frond blade bottom, can generally see sporangiorus growth, in subcircular
Patch shape;Biennial frond almost all grows sporangiorus on whole blades.Holdfast is the rhizoid institute group of dichotomy
Into.Autumn in spore maturity period.2. black kelp frond crineous is to dark brown, keratin, 30-100cm high or more.Thallus is flat
Flat roomy, middle part is slightly thick, and from the pinniform drastic crack of its both sides one to two time, sliver ligule long or longer slightly wrinkles, and edge is general
Have a rough sawn tooth.Petiole bulbous, in cylindrical or slightly flat, 4-12cm long, diameter 3-8mm.There is mucus in the cortical cell of frond
Cavity makees annular arrangement, is 1-2 layers.Marrow is made up of the oviduct silk of indefinite trend.Trip sporangiorus is in ripe thallus
The sliver surface of middle body and side life is formed.Holdfast is made up of the rhizoid of dichotomy branch.Sporangium early summer is formed, spore
Autumn is ripe.3. undaria pinnitafida frond yellowish-brown, soft leather matter, 1-2m high, 50-100cm wide, thallus is flat, and middle part has substantially grand
The middle rib for rising, both sides gradually thin softness forms most pinniform slivers, or is incised in size is uneven, but without sawtooth.Spread on blade face
Many black flecks, are at the epidermotropic opening of mucilage cell.When ripe, petiole both sides below have agaric shape to overlap pleat
The sporophyll of wrinkle, stick-slip meat is thick, thereon close raw sporangium.The holdfast that petiole lower end is made up of the rhizoid of dichotomy.【Property
Taste】It is salty；It is cold.【Return through】Lung；Liver Channel.【Indication】Clearing heat and eliminating phlegm；Cough-relieving；Soothing the liver.Main phlegm-heat cough；The partially high head of liver-yang
It is dizzy；Headache；Being irritable and getting angry easily；Little sleep disturbed by dreams.【Take passages】《Chinese book on Chinese herbal medicine》《Dictionary of medicinal plant》.
Remove Methscopolamine（Norscopolamine）：Molecular formula：C16H19NO4,Molecular weight：289.33, CAS accession number：
4684-28-0。
Tin dilaurate zinc（Zinc Dilaurate）：Molecular formula：C24H46O4Zn,Molecular weight：464.01, CAS accession number：
2452-01-9.Boiling point：296.1°C at 760mmHg（Calculated value）.Flash-point：134.1°C（Calculated value）.
Lithium oleate（Lithium Oleate）：Molecular formula：C18H33LiO2,Molecular weight：288.40, CAS accession number：
7384-22-7.Boiling point：360°C at 760 mmHg（Calculated value）.Flash-point：270.1°C（Calculated value）.
3 chemical constitutions of bulk drug：
Remove Methscopolamine（Norscopolamine）Tin dilaurate zinc（Zinc Dilaurate）
Lithium oleate（Lithium Oleate）.
The content of the invention
The purpose of the present invention is to overcome the shortcomings of background technology, there is provided one kind effectively treat phthisical pharmaceutical composition and
Its preparation method.
The present invention adopts the following technical scheme that realization：
The composition and weight portion for being made the bulk drug of the phthisical pharmaceutical composition of the treatment be：
6755-6855g of colour of loess a kind of reed mentioned in ancient books, 1246-1346g of Holothuria atra, 3488-3588g of Fructus Corni, 5899-5999g of kelp base,
Remove 52-62g of Methscopolamine, 48-58g of tin dilaurate zinc, 27-37g of lithium oleate.
The phthisical pharmaceutical composition for the treatment of is preferably used in, is made up of the bulk drug of following weight portion：
Colour of loess a kind of reed mentioned in ancient books 6805g, Holothuria atra 1296g, Fructus Corni 3538g, kelp base 5949g, remove Methscopolamine 57g, tin dilaurate
Zinc 53g, lithium oleate 32g.
One kind treats phthisical pharmaceutical composition, it is characterised in that pharmaceutical composition can be using the routine side of galenic pharmacy
Method prepares piece agent or capsule or dripping pill.
One kind treats phthisical pharmaceutical composition, it is characterised in that pharmaceutical composition and controlling that chemical drugs or Chinese medicine are constituted
Treat tuberculosis drugs.
A kind of preparation method for treating phthisical pharmaceutical composition, it is characterised in that prepare as follows：
The composition and weight portion of bulk drug be：6755-6855g of colour of loess a kind of reed mentioned in ancient books, 1246-1346g of Holothuria atra, Fructus Corni 3488-
3588g, 5899-5999g of kelp base, go 52-62g of Methscopolamine, 48-58g of tin dilaurate zinc, lithium oleate 27-
37g；
Preparation method：
（1）By bulk drug proportioning fetch earth the root of large-flowered skullcap, Holothuria atra, Fructus Corni, kelp base, go Methscopolamine, tin dilaurate zinc, lithium oil
Hydrochlorate, mixes, and with the ethanol of weight percent concentration 35% as solvent, is taken in 32 DEG C of temperature extractions, and extraction time is 9 times, is carried every time
The time is taken for 32 hours, each solvent load is 42 times of bulk drug gross weight, and filtration obtains dregs of a decoction A and extract solution A, extract solution A
Ethanol is reclaimed, relative density 1.12 is concentrated into, filtered, liquid is first washed with water, then use by LSA-40 large pore resin absorption columns
The ethanol solution of weight percent concentration 48% elutes LSA-40 large pore resin absorption columns, collects the ethanol of weight percent concentration 48% and washes
De- liquid, reclaims ethanol, and concentrate drying obtains final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 51% as solvent, heating and refluxing extraction 4 times is carried every time
The time is taken for 1.8 hours, each solvent load is 11 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.13 is concentrated into, filtered, liquid is first washed with water, then use weight by DM11 large pore resin absorption columns
The ethanol solution of percent concentration 67% elutes DM11 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 67%,
Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Preferred a kind of preparation method for treating phthisical pharmaceutical composition, it is characterised in that prepare as follows：
The composition and weight portion of bulk drug be：Colour of loess a kind of reed mentioned in ancient books 6805g, Holothuria atra 1296g, Fructus Corni 3538g, kelp base 5949g, go
Methscopolamine 57g, tin dilaurate zinc 53g, lithium oleate 32g；
Preparation method：
（1）By bulk drug proportioning fetch earth the root of large-flowered skullcap, Holothuria atra, Fructus Corni, kelp base, go Methscopolamine, tin dilaurate zinc, lithium oil
Hydrochlorate, mixes, and with the ethanol of weight percent concentration 35% as solvent, is taken in 32 DEG C of temperature extractions, and extraction time is 9 times, is carried every time
The time is taken for 32 hours, each solvent load is 42 times of bulk drug gross weight, and filtration obtains dregs of a decoction A and extract solution A, extract solution A
Ethanol is reclaimed, relative density 1.12 is concentrated into, filtered, liquid is first washed with water, then use by LSA-40 large pore resin absorption columns
The ethanol solution of weight percent concentration 48% elutes LSA-40 large pore resin absorption columns, collects the ethanol of weight percent concentration 48% and washes
De- liquid, reclaims ethanol, and concentrate drying obtains final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 51% as solvent, heating and refluxing extraction 4 times is carried every time
The time is taken for 1.8 hours, each solvent load is 11 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.13 is concentrated into, filtered, liquid is first washed with water, then use weight by DM11 large pore resin absorption columns
The ethanol solution of percent concentration 67% elutes DM11 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 67%,
Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
A kind of preparation method for treating phthisical pharmaceutical composition, it is characterised in that pharmaceutical composition can use preparation
Conventional method prepares piece agent or capsule or dripping pill.
A kind of preparation method for treating phthisical pharmaceutical composition, it is characterised in that pharmaceutical composition and chemical drugs or in
Medicine composition treatment tuberculosis drugs.
Specific embodiment
Embodiment 1：Treat phthisical pharmaceutical composition and preparation method thereof
The composition and weight portion for treating the bulk drug of phthisical pharmaceutical composition be：Colour of loess a kind of reed mentioned in ancient books 6805g, Holothuria atra 1296g, mountain
Fruit of medicinal cornel 3538g, kelp base 5949g, remove Methscopolamine 57g, tin dilaurate zinc 53g, lithium oleate 32g；
Preparation method：
（1）By bulk drug proportioning fetch earth the root of large-flowered skullcap, Holothuria atra, Fructus Corni, kelp base, go Methscopolamine, tin dilaurate zinc, lithium oil
Hydrochlorate, mixes, and with the ethanol of weight percent concentration 35% as solvent, is taken in 32 DEG C of temperature extractions, and extraction time is 9 times, is carried every time
The time is taken for 32 hours, each solvent load is 42 times of bulk drug gross weight, and filtration obtains dregs of a decoction A and extract solution A, extract solution A
Ethanol is reclaimed, relative density 1.12 is concentrated into, filtered, liquid is first washed with water, then use by LSA-40 large pore resin absorption columns
The ethanol solution of weight percent concentration 48% elutes LSA-40 large pore resin absorption columns, collects the ethanol of weight percent concentration 48% and washes
De- liquid, reclaims ethanol, and concentrate drying obtains final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 51% as solvent, heating and refluxing extraction 4 times is carried every time
The time is taken for 1.8 hours, each solvent load is 11 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.13 is concentrated into, filtered, liquid is first washed with water, then use weight by DM11 large pore resin absorption columns
The ethanol solution of percent concentration 67% elutes DM11 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 67%,
Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 2：Treat phthisical pharmaceutical composition and preparation method thereof
The composition and weight portion for treating the bulk drug of phthisical pharmaceutical composition be：Colour of loess a kind of reed mentioned in ancient books 6755g, Holothuria atra 1346g, mountain
Fruit of medicinal cornel 3488g, kelp base 5999g, remove Methscopolamine 52g, tin dilaurate zinc 58g, lithium oleate 27g；
Preparation method：
（1）By bulk drug proportioning fetch earth the root of large-flowered skullcap, Holothuria atra, Fructus Corni, kelp base, go Methscopolamine, tin dilaurate zinc, lithium oil
Hydrochlorate, mixes, and with the ethanol of weight percent concentration 35% as solvent, is taken in 32 DEG C of temperature extractions, and extraction time is 9 times, is carried every time
The time is taken for 32 hours, each solvent load is 42 times of bulk drug gross weight, and filtration obtains dregs of a decoction A and extract solution A, extract solution A
Ethanol is reclaimed, relative density 1.12 is concentrated into, filtered, liquid is first washed with water, then use by LSA-40 large pore resin absorption columns
The ethanol solution of weight percent concentration 48% elutes LSA-40 large pore resin absorption columns, collects the ethanol of weight percent concentration 48% and washes
De- liquid, reclaims ethanol, and concentrate drying obtains final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 51% as solvent, heating and refluxing extraction 4 times is carried every time
The time is taken for 1.8 hours, each solvent load is 11 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.13 is concentrated into, filtered, liquid is first washed with water, then use weight by DM11 large pore resin absorption columns
The ethanol solution of percent concentration 67% elutes DM11 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 67%,
Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 3：Treat phthisical pharmaceutical composition and preparation method thereof
The composition and weight portion for treating the bulk drug of phthisical pharmaceutical composition be：Colour of loess a kind of reed mentioned in ancient books 6855g, Holothuria atra 1246g, mountain
Fruit of medicinal cornel 3588g, kelp base 5899g, remove Methscopolamine 62g, tin dilaurate zinc 48g, lithium oleate 37g；
Preparation method：
（1）By bulk drug proportioning fetch earth the root of large-flowered skullcap, Holothuria atra, Fructus Corni, kelp base, go Methscopolamine, tin dilaurate zinc, lithium oil
Hydrochlorate, mixes, and with the ethanol of weight percent concentration 35% as solvent, is taken in 32 DEG C of temperature extractions, and extraction time is 9 times, is carried every time
The time is taken for 32 hours, each solvent load is 42 times of bulk drug gross weight, and filtration obtains dregs of a decoction A and extract solution A, extract solution A
Ethanol is reclaimed, relative density 1.12 is concentrated into, filtered, liquid is first washed with water, then use by LSA-40 large pore resin absorption columns
The ethanol solution of weight percent concentration 48% elutes LSA-40 large pore resin absorption columns, collects the ethanol of weight percent concentration 48% and washes
De- liquid, reclaims ethanol, and concentrate drying obtains final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 51% as solvent, heating and refluxing extraction 4 times is carried every time
The time is taken for 1.8 hours, each solvent load is 11 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.13 is concentrated into, filtered, liquid is first washed with water, then use weight by DM11 large pore resin absorption columns
The ethanol solution of percent concentration 67% elutes DM11 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 67%,
Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 4：The preparation of tablet
The pharmaceutical composition 305g of Example 1, adds starch 105g, mixes, and granulation is dried, plus microcrystalline cellulose 33g, stearic
Sour magnesium 5g, mixes, and is pressed into 1000, obtains final product medicinal composition tablets.
Embodiment 5：The preparation of capsule
The pharmaceutical composition 308g of Example 2, adds starch 84g, mixes, and granulation is dried, whole grain, adds appropriate magnesium stearate,
Mix, encapsulated 1000, obtain final product medicament composition capsule.
Embodiment 6：The preparation of dripping pill
Weigh (80 DEG C) heating of Macrogol 6000 193g water-baths and boil molten, add the pharmaceutical composition 14g of embodiment 3, fully stir
Mix uniform, with atoleine as cooling agent, put glass tube（4*80cm）In, chilling temperature is 5 DEG C, and drip internal-and external diameter is 7.0/
2.0 (mm/mm), drip is 2.5cm away from liquid level, and drop speed is optimum condition with every point 57 drop, and the cold of dripping pill surface is blotted with cotton
Solidifying agent, obtains final product medicament composition dropping pills.
Embodiment 7：Treat phthisical pharmaceutical composition
The composition and weight portion for treating the bulk drug of phthisical pharmaceutical composition be：
Colour of loess a kind of reed mentioned in ancient books 3280g, kelp base 4520g, remove Methscopolamine 35g, tin dilaurate zinc 25g, lithium oleate 20g.
Embodiment 8：Treat phthisical pharmaceutical composition
The composition and weight portion for treating the bulk drug of phthisical pharmaceutical composition be：
Colour of loess a kind of reed mentioned in ancient books 3230g, kelp base 4570g, remove Methscopolamine 30g, tin dilaurate zinc 30g, lithium oleate 15g.
Embodiment 9：Treat phthisical pharmaceutical composition
The composition and weight portion for treating the bulk drug of phthisical pharmaceutical composition be：
Colour of loess a kind of reed mentioned in ancient books 3330g, kelp base 4470g, remove Methscopolamine 40g, tin dilaurate zinc 20g, lithium oleate 25g.
Experimental example 1：Treat phthisical experimental study
1 data and method
1 .1 physical data chooses 82 lungers for accepting for medical treatment Shandong Prov. Hospital in December, 2014 in December, 2015,
Imageological examination result and bacteria cultivation results meet sixth version《Clinical practice》Specified in phthisical diagnostic criteria.Exclude
Standard：1. there is serious liver kidney cardio-pulmonary function obstacle；2. pulmonary tuberculosis has occurred together serious complication；3. gestation or nursing period woman
Female；4. related drugs allergy sufferers.82 patients are divided into observation group and control group, each 41 by the difference according to therapeutic scheme.See
Examine man 18, female 23 in group；39-70 years old ages, average (52.5 ± 11.7) year；The course of disease 6-29 months, average (13.6
± 1.9) individual month.Man 19, female 22 in control group；40-69 years old ages, average (52.8 ± 11.8) year；The course of disease 6~30
Month, average (13.7 ± 1.7) individual month.No significant difference (the P such as two groups of patient ages, courses of disease>0.05), with comparable
Property.Two groups of patients and its family members sign Informed Consent Form before the treatment.
1 .2 treatment methods
1 .2.1 control groups use isoniazid, rifampin, pyrazinamide ordinary combination therapeutic regimen, and 3 kinds of medicines are oral, often
Secondary isoniazid 900mg, rifampin 600mg, pyrazinamide 2g, the next day 1 time, treat 2 months.
1 .2 .2 observation groups give the oral pharmaceutical composition on the basis of control group（The pharmaceutical composition of embodiment 1
Lot number 20141019）, each 1.5g, 3 times a day.Point Morning Afternoon Evening is taken for 3 times, continuous use 1.5 months.
After 1 .3 efficacy assessment standards are continuously treated 1.5 months, formulated according to respirology branch of Chinese Medical Association
《Tuberculosis treatment effective evaluation standard》Therapeutic effect is divided into healing, enabledisable.Cure：Clinical symptom disappearance, iconography
Inspection result shows diseased region shadow-free and no active, continuous 3 Sputum culturings (each gap periods >=2 week) tuberculosis bar
Bacterium is negative；Effectively：Clinical symptoms are relieved, and pulmonary shadow is obviously reduced, but Sputum culturing result is the positive；It is invalid：It is clinical
Symptom is not improved, and Sputum culturing result is feminine gender.Total effective rate=(curing number of cases+effective number of cases)/total number of cases ×
100%.A situation arises for adverse reaction in observing two groups of patient treatment procedures.
1 .4 statistical methods data are processed with SPSS18.0 statistical softwares, and qualitative data is with percentage table
Show, performing check is compared between group. P<0.05 is that difference is statistically significant.
2 results
2 .1 therapeutic effects observation groups treatment total effective rate is higher than control group, the statistically significant (P of difference< 0.05).It is shown in Table
1。
There is no serious adverse reaction in 2 two groups of .2 adverse reactions therapeutic processes.
Table 1 two groups of patient outcomes compare (n, %)
| Group | n | Cure | Effectively | It is invalid | Total effective rate |
| Observation group | 41 | 20 | 18 | 3 | 92.68%* |
| Control group | 41 | 14 | 19 | 8 | 80.48% |
Experimental example 2：Typical case
Clump XX, female, 26 years old, there is cough, expectoration, shortness of breath in March, 2015 without inducement in patient, identifies oneself that nothing's wrong at that time,
Rabat is clapped after the exacerbation of symptoms of in April, 2015 in locality to be diagnosed as：The symptomatic treatments, curative effect such as pulmonary tuberculosis and silicosis, front and rear treating tuberculosis
Not good, shortness of breath aggravation, shows through chest film inspection after in May, 2015 fatigue:Pneumothorax, is sent in hospital by household.AD：1st, double lungs
Secondary tuberculosis of lung applies (+) and controls again, and 2, left side hydropneumothorax, 3, pneumoconiosis.On May 16th, 2015 gives the oral pharmaceutical composition
（The pharmaceutical composition lot number 20141019 of embodiment 1）, each 1.5g, 3 times a day.Point Morning Afternoon Evening is taken for 3 times, continuous use 2
Individual month.Cough, expectoration disappear, and shortness of breath symptom substantially mitigates, and film making pneumothorax absorbs and disappears, and pulmonary lesionses also take an evident turn for the better.It is good
Institute is produced, continues to take medicine 1 month after discharge, afterwards multiple Effect of follow-up visit by telephone, without stable diseases such as cough, expectorations.
What XX, man, 39 years old, lunger, fall ill 2 years, cardinal symptom：Cough, expectoration, shortness of breath, rabat are diagnosed as：Lung
The symptomatic treatments such as tuberculosis and silicosis, front and rear treating tuberculosis, unsatisfactory curative effect, on October 11st, 2015 gives the oral pharmaceutical composition
（The pharmaceutical composition lot number 20141019 of embodiment 1）, each 1.5g, 3 times a day.Point Morning Afternoon Evening is taken for 3 times, continuous use 2
Individual month.Cough, expectoration disappear, and shortness of breath symptom substantially mitigates, and film making pneumothorax absorbs and disappears, and pulmonary lesionses also take an evident turn for the better.It is good
Institute is produced, continues to take medicine 1 month after discharge, afterwards multiple Effect of follow-up visit by telephone, without stable diseases such as cough, expectorations.
Claims (8)
1. one kind treats phthisical pharmaceutical composition, it is characterised in that be made the composition and weight of the bulk drug of the pharmaceutical composition
Measuring part is：
6755-6855g of colour of loess a kind of reed mentioned in ancient books, 1246-1346g of Holothuria atra, 3488-3588g of Fructus Corni, 5899-5999g of kelp base,
Remove 52-62g of Methscopolamine, 48-58g of tin dilaurate zinc, 27-37g of lithium oleate.
2. one kind treats phthisical pharmaceutical composition according to claim 1, it is characterised in that be made the pharmaceutical composition
Bulk drug composition and weight portion be：
Colour of loess a kind of reed mentioned in ancient books 6805g, Holothuria atra 1296g, Fructus Corni 3538g, kelp base 5949g, remove Methscopolamine 57g, tin dilaurate
Zinc 53g, lithium oleate 32g.
3. one kind treats phthisical pharmaceutical composition according to claim 1, it is characterised in that pharmaceutical composition can be adopted
Piece agent or capsule or dripping pill are prepared with the conventional method of galenic pharmacy.
4. one kind treats phthisical pharmaceutical composition according to claim 1, it is characterised in that pharmaceutical composition and chemistry
The treatment tuberculosis drugs of medicine or Chinese medicine composition.
5. a kind of preparation method for treating phthisical pharmaceutical composition, it is characterised in that prepare as follows：
The composition and weight portion of bulk drug be：6755-6855g of colour of loess a kind of reed mentioned in ancient books, 1246-1346g of Holothuria atra, Fructus Corni 3488-
3588g, 5899-5999g of kelp base, go 52-62g of Methscopolamine, 48-58g of tin dilaurate zinc, lithium oleate 27-
37g；
Preparation method：
（1）By bulk drug proportioning fetch earth the root of large-flowered skullcap, Holothuria atra, Fructus Corni, kelp base, go Methscopolamine, tin dilaurate zinc, lithium oil
Hydrochlorate, mixes, and with the ethanol of weight percent concentration 35% as solvent, is taken in 32 DEG C of temperature extractions, and extraction time is 9 times, is carried every time
The time is taken for 32 hours, each solvent load is 42 times of bulk drug gross weight, and filtration obtains dregs of a decoction A and extract solution A, extract solution A
Ethanol is reclaimed, relative density 1.12 is concentrated into, filtered, liquid is first washed with water, then use by LSA-40 large pore resin absorption columns
The ethanol solution of weight percent concentration 48% elutes LSA-40 large pore resin absorption columns, collects the ethanol of weight percent concentration 48% and washes
De- liquid, reclaims ethanol, and concentrate drying obtains final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 51% as solvent, heating and refluxing extraction 4 times is carried every time
The time is taken for 1.8 hours, each solvent load is 11 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.13 is concentrated into, filtered, liquid is first washed with water, then use weight by DM11 large pore resin absorption columns
The ethanol solution of percent concentration 67% elutes DM11 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 67%,
Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
6. a kind of preparation method for treating phthisical pharmaceutical composition according to claim 5, it is characterised in that by as follows
It is prepared by step：
The composition and weight portion of bulk drug be：Colour of loess a kind of reed mentioned in ancient books 6805g, Holothuria atra 1296g, Fructus Corni 3538g, kelp base 5949g, go
Methscopolamine 57g, tin dilaurate zinc 53g, lithium oleate 32g；
Preparation method：
（1）By bulk drug proportioning fetch earth the root of large-flowered skullcap, Holothuria atra, Fructus Corni, kelp base, go Methscopolamine, tin dilaurate zinc, lithium oil
Hydrochlorate, mixes, and with the ethanol of weight percent concentration 35% as solvent, is taken in 32 DEG C of temperature extractions, and extraction time is 9 times, is carried every time
The time is taken for 32 hours, each solvent load is 42 times of bulk drug gross weight, and filtration obtains dregs of a decoction A and extract solution A, extract solution A
Ethanol is reclaimed, relative density 1.12 is concentrated into, filtered, liquid is first washed with water, then use by LSA-40 large pore resin absorption columns
The ethanol solution of weight percent concentration 48% elutes LSA-40 large pore resin absorption columns, collects the ethanol of weight percent concentration 48% and washes
De- liquid, reclaims ethanol, and concentrate drying obtains final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 51% as solvent, heating and refluxing extraction 4 times is carried every time
The time is taken for 1.8 hours, each solvent load is 11 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.13 is concentrated into, filtered, liquid is first washed with water, then use weight by DM11 large pore resin absorption columns
The ethanol solution of percent concentration 67% elutes DM11 large pore resin absorption columns, collects the ethanol eluate of weight percent concentration 67%,
Ethanol is reclaimed, concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
7. a kind of preparation method for treating phthisical pharmaceutical composition according to claim 5, it is characterised in that medicine group
Compound can prepare piece agent or capsule or dripping pill using the conventional method of galenic pharmacy.
8. a kind of preparation method for treating phthisical pharmaceutical composition according to claim 5, it is characterised in that medicine group
Compound and chemical drugs or Chinese medicine composition treatment tuberculosis drugs.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111714496A (en) * | 2019-03-18 | 2020-09-29 | 深圳市益米诺医药科技有限公司 | Application of small molecular compound in improving host pathogenic bacteria removing capability and application in preparing anti-pathogenic bacteria infection medicine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105168270A (en) * | 2015-09-09 | 2015-12-23 | 济南星懿医药技术有限公司 | Medicine composition for treating phthisis and preparation method thereof |
| CN106109721A (en) * | 2016-06-30 | 2016-11-16 | 济南星懿医药技术有限公司 | The pharmaceutical composition for the treatment of cervical cancer |
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2016
- 2016-12-10 CN CN201611134504.8A patent/CN106727943A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105168270A (en) * | 2015-09-09 | 2015-12-23 | 济南星懿医药技术有限公司 | Medicine composition for treating phthisis and preparation method thereof |
| CN106109721A (en) * | 2016-06-30 | 2016-11-16 | 济南星懿医药技术有限公司 | The pharmaceutical composition for the treatment of cervical cancer |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111714496A (en) * | 2019-03-18 | 2020-09-29 | 深圳市益米诺医药科技有限公司 | Application of small molecular compound in improving host pathogenic bacteria removing capability and application in preparing anti-pathogenic bacteria infection medicine |
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Application publication date: 20170531 |