CN116699123B - Method for detecting zearalenone in grains and products thereof - Google Patents
Method for detecting zearalenone in grains and products thereof Download PDFInfo
- Publication number
- CN116699123B CN116699123B CN202310965675.9A CN202310965675A CN116699123B CN 116699123 B CN116699123 B CN 116699123B CN 202310965675 A CN202310965675 A CN 202310965675A CN 116699123 B CN116699123 B CN 116699123B
- Authority
- CN
- China
- Prior art keywords
- seeds
- fixedly connected
- water
- sample
- grains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- MBMQEIFVQACCCH-UHFFFAOYSA-N trans-Zearalenon Natural products O=C1OC(C)CCCC(=O)CCCC=CC2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-UHFFFAOYSA-N 0.000 title claims abstract description 18
- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 123
- 238000012216 screening Methods 0.000 claims abstract description 32
- 239000007921 spray Substances 0.000 claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 238000007873 sieving Methods 0.000 claims abstract description 8
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 18
- 239000000047 product Substances 0.000 claims description 14
- 239000011259 mixed solution Substances 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 3
- 230000010355 oscillation Effects 0.000 claims description 3
- 238000011895 specific detection Methods 0.000 claims description 3
- 230000005484 gravity Effects 0.000 abstract description 9
- 235000013339 cereals Nutrition 0.000 description 16
- 101150114468 TUB1 gene Proteins 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 240000008042 Zea mays Species 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 229930191978 Gibberellin Natural products 0.000 description 4
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 4
- 239000003448 gibberellin Substances 0.000 description 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 3
- 241000223218 Fusarium Species 0.000 description 3
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 241000209763 Avena sativa Species 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- 241000221779 Fusarium sambucinum Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- -1 fusarium roseum Chemical compound 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
- B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
- B03B5/28—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation
- B03B5/30—Washing granular, powdered or lumpy materials; Wet separating by sink-float separation using heavy liquids or suspensions
- B03B5/36—Devices therefor, other than using centrifugal force
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a detection method of zearalenone in grains and products thereof, and relates to the technical field of grain detection. The method comprises the following specific steps: screening out grain seeds to be detected by using a screening device; crushing the screened sample seeds, and sieving the crushed seeds; taking 0.98-1.02g of sample seeds after sieving, and placing the sample seeds into a centrifuge tube; adding a sample extracting solution into the centrifuge tube; the mould seeds with smaller specific gravity can float upwards under the buoyancy of water, normal seeds with larger specific gravity can stay in the screen drum, and the floating mould seeds can float on the water surface, so that preliminary screening of the mould seeds and normal seeds can be completed; the clear water and the gas are intermittently injected into the water feeding cavity, so that the spray pipe can intermittently spray disturbance water flow, seeds in the screen drum can be intermittently disturbed, and the mould-forming seeds can be sufficiently screened out through multiple intermittent disturbance.
Description
Technical Field
The invention belongs to the technical field of grain detection, and particularly relates to a detection method of zearalenone in grains and products thereof.
Background
The zearalenone is mainly produced by fusarium graminearum, and can also produce the toxin by multiple fusarium such as fusarium roseum, fusarium bead-channeling, fusarium trilineum and the like; corn gibberellin is also present in many crops such as wheat, soybean, etc., and is mainly contaminated with corn, wheat, rice, barley, millet, oat, etc., where the reason for the production of corn gibberellin is mainly the mildew of the grain crop during storage, and the higher content of corn gibberellin in the mildewed grain crop, therefore, corn gibberellin detection is required for the mildewed grain crop.
In the prior art, a fluorescence immunoassay method is generally adopted to detect zearalenone in grains, when grain crops are pretreated, in order to ensure detection accuracy, mildewed seeds and normal seeds in grain samples are generally required to be distinguished, and preferably, the mildewed seeds are analyzed and detected, when the sample seeds are screened by the existing detection device, the mildewed seeds and the normal seeds can not be distinguished, and when the mildewed seeds are mixed with the normal seeds, the overall detection accuracy of follow-up samples can be affected.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for detecting zearalenone in grains and products thereof, which can overcome or at least partially solve the problems.
In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that: a detection method of zearalenone in grains and products thereof comprises the following specific detection steps:
step one, screening out grain seeds to be detected by using a screening device;
crushing the sample seeds screened in the step II;
step three, sieving the seeds crushed in the step two;
step four, 0.98-1.02g of sample seeds after sieving are taken and put into a centrifuge tube;
step five, adding a sample extracting solution into the centrifuge tube;
step six, oscillating the sample mixed solution for 4-6min by using a vortex oscillator;
step seven, after the oscillation is finished, placing the sample mixed solution in a centrifugal machine for centrifugation for 1-2min;
step eight, taking the supernatant after centrifugation, diluting the supernatant in a sample diluent, and uniformly mixing for later use;
step nine, placing the test strip on a 37 ℃ incubator for 100ul sample addition;
step ten, reacting for 9-11min on an incubator;
and step eleven, reading analysis is carried out to obtain a detection result.
Preferably, the sample extracting solution in the fifth step is a mixed solution of acetonitrile and deionized water, and the volume ratio of acetonitrile to deionized water is 9:1.
Preferably, the screening apparatus in the first step includes:
a tub body;
the sliding rod is fixedly connected in the barrel body;
the screen cylinder is connected to the sliding rod in a sliding way;
the blade is fixedly connected to the side wall of the screen drum
The water feeding ring is fixedly connected to the side wall of the barrel body;
the spray pipe is fixedly connected to the water delivery ring, and the water outlet end of the spray pipe is communicated with the locking barrel body;
the water outlet end of the spray pipe is obliquely arranged on the barrel body;
the discharging pipe is fixedly connected to the side wall of the barrel body.
Further, a supporting rod is connected to the side wall of the barrel body in a sliding mode, and the supporting rod abuts against the blade.
Further, the water delivery ring top fixedly connected with connecting cylinder, sliding connection has the drive lead screw on the connecting cylinder, it is connected with the drive plate to rotate on the connecting cylinder, the drive plate with drive lead screw thread match, the first teeth of a cogwheel of fixedly connected with multiunit on the drive plate, fixedly connected with is held against the board on the pole, fixedly connected with multiunit second teeth of a cogwheel on the board, first teeth of a cogwheel with the second teeth of a cogwheel mesh mutually.
Further, a first electromagnet is fixedly connected to the sliding rod, a first magnet is fixedly connected to the inner bottom wall of the screen cylinder, and the first electromagnet and the first magnet attract each other.
In order to further enable the screen cylinder to move downwards, further, the bottom of the sliding rod is fixedly connected with a second electromagnet, the bottom wall of the screen cylinder is fixedly connected with a second magnet, and the second magnet and the second electromagnet are attracted.
In order to ensure that the supporting rod cannot slide off from the barrel body due to excessive sliding when the supporting rod is in sliding reset, a plurality of groups of limiting rods are fixedly connected to the supporting rod, and a plurality of groups of limiting rods are symmetrically arranged on the side wall of the supporting rod.
In order to facilitate the visual observation of the poured water quantity by the staff, further, a graduated scale is fixedly connected to the inner side wall of the barrel body.
In order to improve the water separation efficiency, further, the side wall and the bottom wall of the screen cylinder are provided with screen holes.
After the technical scheme is adopted, compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the mould seeds with smaller specific gravity can float upwards under the buoyancy of water, normal seeds with larger specific gravity can stay in the screen drum, and the floating mould seeds can float on the water surface, so that preliminary screening of the mould seeds and normal seeds can be completed;
the clear water and the gas are intermittently injected into the water feeding cavity, so that the spray pipe can intermittently spray disturbance water flow, seeds in the screen drum can be intermittently disturbed, and the mould-forming seeds can be sufficiently screened out through multiple intermittent disturbance.
2. According to the invention, through water in the water feeding cavity is sprayed out, the floating plate moves downwards due to the reduction of water flow, the floating plate moves downwards to pull the driving screw rod to move downwards, so that the driving plate is driven to rotate reversely, the pushing rod is driven to push inwards, the pushing rod is pushed against the blades, at the moment, the screen cylinder and the rotating water flow generate opposite acting force, the rotating water flow can strike the seeds in the screen cylinder and the cylinder, the seeds in the screen cylinder can overturn and roll again under the impact of the water flow, the seeds in the screen cylinder can be disturbed again, the gap between various seeds can be enlarged again, and the upward floating of moldy seeds in the piled seeds is further facilitated.
Drawings
In the drawings:
fig. 1 is a schematic structural diagram of a screening apparatus according to the present invention;
FIG. 2 is a cross-sectional view of a screening apparatus according to the present invention;
fig. 3 is a schematic diagram of an internal structure of a barrel in the screening device according to the present invention;
FIG. 4 is a schematic diagram showing a connection structure between a slide bar and a screen drum in a screening apparatus according to the present invention;
fig. 5 is a schematic diagram II of a connection structure between a slide bar and a screen drum in the screening device according to the present invention;
FIG. 6 is an enlarged view of part A of the screening apparatus according to the present invention
FIG. 7 is an enlarged schematic view of a portion B of the screening apparatus according to the present invention;
FIG. 8 is an enlarged schematic view of a portion C of a screening apparatus according to the present invention;
FIG. 9 is an enlarged schematic view of a portion D of the screening apparatus according to the present invention;
fig. 10 is a schematic diagram of a cross-sectional structure of a barrel in a screening apparatus according to the present invention;
FIG. 11 is a schematic diagram of a cross-sectional structure of a barrel in a screening apparatus according to the present invention;
FIG. 12 is an enlarged schematic view of the portion E of the screening apparatus according to the present invention;
fig. 13 is an enlarged schematic view of a portion F of the screening apparatus according to the present invention.
In the figure: 1. a tub body; 101. a discharge pipe; 102. an air inlet pipe; 103. a water inlet pipe; 104. a graduated scale; 2. a slide bar; 201. a limiting disc; 202. a first electromagnet; 203. a second electromagnet; 3. a connecting cylinder; 301. a driving plate; 3011. a first gear tooth; 4. a screen drum; 401. a blade; 402. a sieve pore; 403. a second magnet; 404. a first magnet; 5. a water feeding ring; 501. a water feeding cavity; 502. a spray pipe; 503. a floating plate; 5031. driving a screw rod; 504. filling the air bag; 6. a retaining plate; 601. a supporting rod; 6011. a limit rod; 602. and a second gear tooth.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
Example 1: referring to fig. 1-9, a method for detecting zearalenone in grains and products thereof comprises the following specific detection steps:
step one, screening out grain seeds to be detected by using a screening device;
crushing the sample seeds screened in the step II;
step three, sieving the seeds crushed in the step two;
step four, 1g of sample seeds after sieving is taken and put into a centrifuge tube;
step five, adding a sample extracting solution into the centrifuge tube;
step six, oscillating the sample mixed solution for 5min by using a vortex oscillator;
step seven, after the oscillation is finished, placing the sample mixed solution in a centrifugal machine for centrifugation for 1min;
step eight, taking the supernatant after centrifugation, diluting the supernatant in a sample diluent, and uniformly mixing for later use;
step nine, placing the test strip on a 37 ℃ incubator for 100ul sample addition;
step ten, reacting for 10min on an incubator;
and step eleven, reading analysis is carried out to obtain a detection result.
In the fifth step, the sample extracting solution is a mixed solution of acetonitrile and deionized water, and the volume ratio of acetonitrile to deionized water is 9:1.
The screening device in the first step comprises:
a tub 1;
the slide bar 2 is fixedly connected in the barrel body 1;
the screen cylinder 4 is connected to the slide rod 2 in a sliding manner;
blades 401 fixedly connected to the side wall of the screen drum 4
The water feeding ring 5 is fixedly connected to the side wall of the barrel body 1;
the spray pipe 502 is fixedly connected to the water delivery ring 5, and the water outlet end of the spray pipe 502 is communicated with the locking barrel body 1;
the discharging pipe 101 is fixedly connected to the side wall of the barrel body 1.
The top of the slide bar 2 is fixedly connected with a limiting disc 201.
The spray pipe 502 is provided with the multiunit, and the spray pipe 502 evenly distributed of multiunit is on staving 1, and spray pipe 502 slope setting, the spray pipe 502 of slope setting is 30 contained angles with staving 1's center, and contained angle can be set for according to the screening demand.
Referring to fig. 3, by providing an inclined nozzle 502, the flow rate of the water body vortex can be changed to some extent when the nozzle 502 ejects the disturbance water flow.
The jet pipe 502 is provided with a one-way pressure valve, and water sprayed into the tub 1 can not flow back through the one-way pressure valve.
Referring to fig. 1 to 3, when a certain amount of water is injected through the nozzle 502, the liquid level in the tub 1 rises to the bottom of the discharge pipe 101, and when the water flow is swirled, the seeds screened on the water surface are discharged through the discharge pipe 101.
The side wall of the barrel body 1 is connected with a supporting rod 601 in a sliding way, and the supporting rod 601 is propped against the blade 401.
The side wall of the water feeding ring 5 is fixedly connected with a water inlet pipe 103 and an air inlet pipe 102, the water inlet pipe 103 and the air inlet pipe 102 are symmetrically arranged, the air inlet end of the air inlet pipe 102 is externally connected with an external air pump, and the water inlet end of the water inlet pipe 103 is externally connected with an external water pump.
The air inlet pipe 102 and the water inlet pipe 103 are respectively provided with a one-way valve, and water flow and air flow which are conveyed into the water feeding ring 5 can not flow back and escape through the one-way valves arranged on the air inlet pipe 102 and the water inlet pipe 103.
The top of the water feeding ring 5 is fixedly connected with a connecting cylinder 3, the connecting cylinder 3 is connected with a driving screw 5031 in a sliding manner, the connecting cylinder 3 is connected with a driving plate 301 in a rotating manner, the driving plate 301 is matched with the driving screw 5031 in a threaded manner, a plurality of groups of first gear teeth 3011 are fixedly connected to the driving plate 301, a retaining plate 6 is fixedly connected to the retaining rod 601, a plurality of groups of second gear teeth 602 are fixedly connected to the retaining plate 6, and the first gear teeth 3011 are meshed with the second gear teeth 602.
The first electromagnet 202 is fixedly connected to the slide bar 2, the first magnet 404 is fixedly connected to the inner bottom wall of the screen cylinder 4, and the first electromagnet 202 and the first magnet 404 attract each other.
The bottom of the slide bar 2 is fixedly connected with a second electromagnet 203, the bottom wall of the screen cylinder 4 is fixedly connected with a second magnet 403, and the second magnet 403 and the second electromagnet 203 attract each other.
Referring to fig. 5, after the screen drum 4 slides down along the sliding rod 2 for a certain distance, the second magnet 403 on the bottom wall of the screen drum 4 will receive the attraction of the second electromagnet 203, and under the attraction force of the second electromagnet 203, the second magnet 403 will pull the screen drum 4 to move down quickly, so as to further improve the screening efficiency.
After the second magnet 403 and the second electromagnet 203 attract each other, when the screen cylinder 4 is pushed to rotate by the subsequent water flow disturbance, the impact force of the water flow disturbance is larger than the acting force between the second magnet 403 and the second electromagnet 203, so that the screen cylinder 4 can rotate stably.
The supporting rod 601 is fixedly connected with a plurality of groups of limiting rods 6011, and the limiting rods 6011 of the plurality of groups are symmetrically arranged on the side wall of the supporting rod 601.
Referring to fig. 6, the stopper rod 6011 fixedly connected to the stopper rod 601 prevents the stopper rod 601 from being separated from the tub 1 due to excessive sliding when the stopper rod 601 slides outward.
A graduated scale 104 is fixedly connected to the inner side wall of the barrel body 1.
Referring to fig. 3, through the scale 104 fixedly connected to the inner sidewall of the tub 1, a worker can intuitively observe the amount of water added when adding clear water.
Screen holes 402 are formed in the side wall and the bottom wall of the screen drum 4.
Referring to fig. 1 to 9, in use, fresh water is added to the tub 1, and the specific amount of water added can be observed by observing the scale 104 on the inner side wall of the tub 1.
During screening, seeds to be screened are added into the screen cylinder 4, with the continuous addition of the seeds, the screen cylinder 4 will have a downward moving trend under the action of the gravity of the seeds, when the gravity of the seeds and the gravity of the screen cylinder 4 are greater than the suction force of the first electromagnet 202 and the first magnet 404, the screen cylinder 4 will drop instantly, the dropped screen cylinder 4 will slide down along the slide rod 2 with the loaded seeds, the seeds in the rapidly sliding screen cylinder 4 will be in contact with the water sinking into the barrel body 1, the moldy seeds with smaller specific gravity will float upwards under the buoyancy of the water, the normal seeds with larger specific gravity will stay in the screen cylinder 4, and the moldy seeds floating upwards will float on the water surface.
Referring to fig. 1-9, clean water is injected into the water delivery cavity 501 through the water inlet pipe 103, after a certain amount of water is injected, the water is stopped from being input, gas is input into the water delivery cavity 501 through the air inlet pipe 102, along with the input of the gas, the pressure in the water delivery cavity 501 is increased, when the pressure reaches the set threshold value of the one-way pressure valve in the spray pipe 502, the one-way pressure valve is opened, the stored clean water in the water delivery cavity 501 is sprayed out through the spray pipe 502 which is obliquely arranged at the moment, the sprayed water flow can disturb the water in the barrel body 1, vortex-shaped water flow can be generated after the water in the barrel body 1 is disturbed, the vortex-shaped water flow can impact seeds in the screen cylinder 4, the accumulated seeds can be disturbed, and then gaps among the seeds are increased, and the mould seeds mixed in the accumulated seeds are favorably to be floated.
By intermittently injecting clear water and gas into the water supply cavity 501, the jet pipe 502 can intermittently jet disturbance water flow, so that seeds in the screen drum 4 can be intermittently disturbed, and the mildewed seeds can be sufficiently screened out through multiple intermittent disturbance.
Referring to fig. 2-9, in the process of water flow disturbance, the blade 401 is pushed to rotate by the vortex-shaped rotating water flow, the rotating blade 401 can push the screen drum 4 to rotate, when the screen drum 4 rotates, the driving screw 5031 is driven to slide to drive the driving plate 301 meshed with the screw to rotate, the driving plate 301 rotates to drive the first gear teeth 3011 fixedly connected with the driving plate to rotate, the first gear teeth 3011 drives the second gear teeth 602 meshed with the driving plate to move, the abutting plate 6 is pushed to slide by the movement of the abutting plate 601, the abutting rod 601 slides for a certain distance and then abuts against the blade 401, at the moment, the limit of the blade 401 when the abutting rod 601 is received, the water flow in the barrel 1 still rotates in a vortex-shaped manner, at the moment, the rotating water flow in the screen drum 4 and the rotating water flow can generate opposite acting force, the seeds in the screen drum 4 and the barrel can be overturned again under the impact of the water flow, the seeds in the screen drum 4 can be disturbed again, the gaps among the seeds can be enabled again, and the seeds can be further mixed with the gaps between the seeds can be more easily and the accumulated in the mould.
When the supporting rod 601 reversely slides and resets, the supporting rod 601 does not support against the blade 401 any more, and when the spray pipe 502 sprays water again, the water flow can disturb the rotation of the blade 401 again;
seeds in the screen cylinder 4 can be subjected to full disturbance screening through repeated water spraying for many times, moldy seeds in the screen cylinder 4 can be fully screened and floated, and screening efficiency is guaranteed.
Referring to fig. 2, after the screening is completed, water in the barrel 1 is pumped out by an external water pump, the screen cylinder 4 is manually pulled to separate the second magnet 403 from the second electromagnet 203, and the screen cylinder 4 is pulled upwards until the first magnet 404 and the first electromagnet 202 attract each other, and the remaining seeds in the screen cylinder 4 are manually taken out.
Example 2: referring to fig. 1 to 9, a method for detecting zearalenone in grains and products thereof is substantially the same as that of embodiment 1, further, a floating plate 503 is disposed in a water supply cavity 501, and a driving screw 5031 is inserted into a water supply ring 5 and fixedly connected with the floating plate 503.
When the water in the water supply cavity 501 increases, the floating plate 503 in the water supply cavity 501 will move upwards under the buoyancy of the water, and the upward movement of the floating plate 503 will push the driving screw 5031 to move upwards, so as to drive the driving plate 301 to rotate, and further drive the supporting rod 601 to pull outwards.
Referring to fig. 6-9, when the one-way pressure valve in the spray pipe 502 is opened, the water in the water supply cavity 501 is sprayed out, the floating plate 503 will move downwards due to the reduction of the water flow, the floating plate 503 moves downwards to pull the driving screw 5031 to move downwards, and then drive the driving plate 301 to rotate reversely, and further drive the pushing rod 601 to push inwards, the pushed pushing rod 601 will abut against the blade 401, at this time, the sieve cylinder 4 and the rotating water flow will generate opposite acting force, the rotating water flow will strike the sieve cylinder 4 and the seeds in the sieve cylinder 4, the seeds in the sieve cylinder 4 will overturn and roll again under the impact of the water flow, and then the seeds in the sieve cylinder 4 can be disturbed again, the gap between the seeds can be enlarged again, and the upward floating of the moldy seeds mixed in the accumulated seeds is further facilitated.
Example 3: referring to fig. 1 to 13, a method for detecting zearalenone in grains and products thereof is basically the same as that of embodiment 2, except that the floating plate 503 is an annular plate, the bottom wall of the floating plate 503 is fixedly connected with a filling air bag 504, and the side wall of the filling air bag 504 is closely adhered to the side wall of the water delivery cavity 501.
The filling bladder 504 is an elastic bladder.
Referring to fig. 10-12, before water injection, the filling bladder 504 is tightly attached to the side wall of the water delivery chamber 501, and the filling bladder 504 blocks the nozzle 502;
when a certain amount of water is injected, the water pushes the filling air bag 504 and the floating plate 503 to move upwards, so that the driving screw rod 5031 can be driven to slide, when water is continuously injected into the water feeding cavity 501, the added water can squeeze the filling air bag 504, the filling air bag 504 is squeezed to deform, the filling air bag 504 does not block the spray pipe 502 any more, the water in the water feeding cavity 501 is sprayed out through the spray pipe 502, and the sprayed water can disturb the water in the barrel body 1, so that vortex-shaped water flow is generated.
When water in the water supply cavity 501 is sprayed out through the spray pipe 502, the water in the water supply cavity 501 is reduced, the floating plate 503 and the filling air bag 504 move downwards, the extruded filling air bag 504 deforms and resets, the filling air bag 504 bulges again, and the bulged filling air bag 504 plugs the spray pipe 502 again.
The present invention is not limited to the above embodiments, but is capable of being modified or varied in many ways, and still fall within the scope of the present invention.
Claims (7)
1. A detection method of zearalenone in grains and products thereof is characterized by comprising the following specific detection steps:
step one, screening out grain seeds to be detected by using a screening device;
crushing the sample seeds screened in the first step;
step three, sieving the seeds crushed in the step two;
step four, 0.98-1.02g of sample seeds after sieving are taken and put into a centrifuge tube;
step five, adding a sample extracting solution into the centrifuge tube;
step six, oscillating the sample mixed solution for 4-6min by using a vortex oscillator;
step seven, after the oscillation is finished, placing the sample mixed solution in a centrifugal machine for centrifugation for 1-2min;
step eight, taking the supernatant after centrifugation, diluting the supernatant in a sample diluent, and uniformly mixing for later use;
step nine, placing the test strip on a 37 ℃ incubator for 100ul sample addition;
step ten, reacting for 9-11min on an incubator;
step eleven, reading analysis is carried out to obtain a detection result;
the screening device in the first step comprises:
a barrel body (1);
the sliding rod (2) is fixedly connected in the barrel body (1);
the screen cylinder (4) is connected to the sliding rod (2) in a sliding manner;
blades (401) fixedly connected to the side wall of the screen drum (4)
A water feeding ring (5) fixedly connected to the side wall of the barrel body (1);
the spray pipe (502) is fixedly connected to the water delivery ring (5), and the water outlet end of the spray pipe (502) is communicated with the locking barrel body (1);
the water outlet end of the spray pipe (502) is obliquely arranged on the barrel body (1);
the discharging pipe (101) is fixedly connected to the side wall of the barrel body (1);
a supporting rod (601) is connected to the side wall of the barrel body (1) in a sliding manner, and the supporting rod (601) abuts against the blade (401);
the water supply ring is characterized in that the top of the water supply ring (5) is fixedly connected with a connecting cylinder (3), the connecting cylinder (3) is connected with a driving screw rod (5031) in a sliding mode, the connecting cylinder (3) is connected with a driving plate (301) in a rotating mode, the driving plate (301) is in threaded matching with the driving screw rod (5031), the driving plate (301) is fixedly connected with a plurality of groups of first gear teeth (3011), the supporting rod (601) is fixedly connected with a supporting plate (6), the supporting plate (6) is fixedly connected with a plurality of groups of second gear teeth (602), and the first gear teeth (3011) are meshed with the second gear teeth (602).
2. The method for detecting zearalenone in grains and products thereof according to claim 1, wherein the sample extracting solution in the fifth step is a mixed solution of acetonitrile and deionized water, and the volume ratio of acetonitrile to deionized water is 9:1.
3. The method for detecting zearalenone in grains and products thereof according to claim 1, wherein a first electromagnet (202) is fixedly connected to the sliding rod (2), a first magnet (404) is fixedly connected to the inner bottom wall of the screen cylinder (4), and the first electromagnet (202) and the first magnet (404) attract each other.
4. The method for detecting zearalenone in grains and products thereof according to claim 3, wherein the bottom of the sliding rod (2) is fixedly connected with a second electromagnet (203), the bottom wall of the screen cylinder (4) is fixedly connected with a second magnet (403), and the second magnet (403) and the second electromagnet (203) are attracted.
5. The method for detecting zearalenone in grains and products thereof according to claim 1, wherein a plurality of groups of limit rods (6011) are fixedly connected to the supporting rod (601), and the limit rods (6011) are symmetrically arranged on the side wall of the supporting rod (601).
6. The method for detecting zearalenone in grains and products thereof according to claim 1, wherein a graduated scale (104) is fixedly connected to the inner side wall of the barrel body (1).
7. The method for detecting zearalenone in grains and products thereof according to claim 6, wherein the side wall and bottom wall of the screen cylinder (4) are provided with screen holes (402).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310965675.9A CN116699123B (en) | 2023-08-02 | 2023-08-02 | Method for detecting zearalenone in grains and products thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310965675.9A CN116699123B (en) | 2023-08-02 | 2023-08-02 | Method for detecting zearalenone in grains and products thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116699123A CN116699123A (en) | 2023-09-05 |
| CN116699123B true CN116699123B (en) | 2023-10-03 |
Family
ID=87826071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310965675.9A Active CN116699123B (en) | 2023-08-02 | 2023-08-02 | Method for detecting zearalenone in grains and products thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116699123B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB791520A (en) * | 1953-12-24 | 1958-03-05 | Stamicarbon | Improvements in and relating to the wet screening of solid particles and apparatus therefor |
| KR20130037489A (en) * | 2011-10-06 | 2013-04-16 | 대한민국(농촌진흥청장) | Seeds separating apparatus applied water immerged method |
| CN206604751U (en) * | 2017-03-26 | 2017-11-03 | 成都大学 | A kind of centrifugal seed water choosing device |
| CN107462641A (en) * | 2017-07-17 | 2017-12-12 | 内蒙古蒙牛乳业（集团）股份有限公司 | Method that is a kind of while detecting ochratoxin A and zearalenone |
| CN108607677A (en) * | 2018-04-20 | 2018-10-02 | 道真仡佬族苗族自治县好未来中药材农民专业合作社 | Lip river Radix Codonopsis seed screening plant |
| CN209156554U (en) * | 2018-10-22 | 2019-07-26 | 王增文 | A kind of wash seeds device using reverse impact power |
| CN114097348A (en) * | 2021-11-26 | 2022-03-01 | 宁夏农产品质量标准与检测技术研究所(宁夏农产品质量监测中心) | Corn seed detection screening device for planting |
-
2023
- 2023-08-02 CN CN202310965675.9A patent/CN116699123B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB791520A (en) * | 1953-12-24 | 1958-03-05 | Stamicarbon | Improvements in and relating to the wet screening of solid particles and apparatus therefor |
| KR20130037489A (en) * | 2011-10-06 | 2013-04-16 | 대한민국(농촌진흥청장) | Seeds separating apparatus applied water immerged method |
| CN206604751U (en) * | 2017-03-26 | 2017-11-03 | 成都大学 | A kind of centrifugal seed water choosing device |
| CN107462641A (en) * | 2017-07-17 | 2017-12-12 | 内蒙古蒙牛乳业（集团）股份有限公司 | Method that is a kind of while detecting ochratoxin A and zearalenone |
| CN108607677A (en) * | 2018-04-20 | 2018-10-02 | 道真仡佬族苗族自治县好未来中药材农民专业合作社 | Lip river Radix Codonopsis seed screening plant |
| CN209156554U (en) * | 2018-10-22 | 2019-07-26 | 王增文 | A kind of wash seeds device using reverse impact power |
| CN114097348A (en) * | 2021-11-26 | 2022-03-01 | 宁夏农产品质量标准与检测技术研究所(宁夏农产品质量监测中心) | Corn seed detection screening device for planting |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116699123A (en) | 2023-09-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2014184766A1 (en) | Method and apparatus for removing parasites from fish | |
| CN104075907A (en) | Food sampling machine with sample splitter | |
| CN116699123B (en) | Method for detecting zearalenone in grains and products thereof | |
| CN107297381B (en) | Biogas residue processing device for improving non-point source pollution | |
| CN207703825U (en) | A kind of food inspection system | |
| CN103564618A (en) | Juice extractor and method for operating juice extractor | |
| CN112415154A (en) | Flour gluten degree detection device | |
| CN110495415B (en) | Structure-improved aquaculture net cage | |
| CN203965180U (en) | The grain sampler of tool sample spliter | |
| CN109692808A (en) | Device is used in a kind of elutriation of Full-automatic water-saving grain | |
| CN212795426U (en) | Dry-mixed mortar storing and mixing device | |
| CN201001381Y (en) | Melon breaking and seeds taking machine melon skin and melon seed separating device | |
| CN112391244A (en) | Beer with malt fragrance | |
| CN210375905U (en) | Agricultural product pesticide residue detection device | |
| CN211865465U (en) | Novel centrifugal machine | |
| CN216321165U (en) | Quick preparation facilities of calcium formate solution | |
| CN112161750A (en) | Positive pressure checking method for sealing performance of vacuum pugging machine | |
| CN112557462A (en) | Resistance type grain moisture detector feeding anti-blocking device | |
| CN109692809A (en) | A kind of Full-automatic water-saving grain elutriation application method of device | |
| CN106216080B (en) | Hydrocone type sorting equipment and complete sorting system | |
| CN218282651U (en) | Screening device for spirulina pretreatment | |
| CN116140027B (en) | Gold tailing waste residue grinder | |
| CN108077976A (en) | Paint finishing after thousand classification of one kind | |
| CN115228546B (en) | Spore powder's broken wall equipment | |
| CN211069966U (en) | Feeder is used in chemical fertilizer processing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |