CN116682715A - Freezing transmission sample pole - Google Patents
Freezing transmission sample pole Download PDFInfo
- Publication number
- CN116682715A CN116682715A CN202310533307.7A CN202310533307A CN116682715A CN 116682715 A CN116682715 A CN 116682715A CN 202310533307 A CN202310533307 A CN 202310533307A CN 116682715 A CN116682715 A CN 116682715A
- Authority
- CN
- China
- Prior art keywords
- rod
- sample
- cup
- inner cup
- transmission
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000005540 biological transmission Effects 0.000 title claims abstract description 94
- 238000007710 freezing Methods 0.000 title claims abstract description 51
- 230000008014 freezing Effects 0.000 title claims abstract description 50
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000005057 refrigeration Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 12
- 238000000605 extraction Methods 0.000 claims description 45
- 238000007789 sealing Methods 0.000 claims description 31
- 230000002457 bidirectional effect Effects 0.000 claims description 11
- 230000000149 penetrating effect Effects 0.000 claims description 11
- 238000001816 cooling Methods 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 59
- 238000005086 pumping Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 238000004891 communication Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000000740 bleeding effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000013386 optimize process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
- H01J37/02—Details
- H01J37/20—Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
- H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The embodiment of the application discloses a freezing transmission sample rod, which comprises a rod body, a cold guide rod, a rod head, an inner cup and an outer cup, wherein the rod head is arranged at the head of the rod body, a first cavity gap is formed between the cold guide rod and the rod body, the inner cup is arranged inside the outer cup and is used for loading liquid nitrogen, the outer cup is arranged at the tail of the rod body, a second cavity gap communicated with the first cavity gap is formed between the inner cup and the outer cup, and an air exhaust assembly communicated with the second cavity gap is arranged on the outer cup. According to the application, the inner cup is arranged to place liquid nitrogen to provide a refrigeration source, the inner cup body and the outer cup body are combined to provide a vacuum cold guide cavity gap, and the outer cup body is provided with the vacuum drawing component for drawing to form a vacuum environment.
Description
Technical Field
The application relates to the technical field of material analysis and test, in particular to a freezing transmission sample rod.
Background
In the structural characteristics of the research material at the nanometer or atomic level, a transmission electron microscope is often needed, a sample rod is needed to be used for guiding a sample to the transmission electron microscope for observation in the material analysis research at home and abroad, the use function of the sample rod is influenced by the microscopic observation environment of the sample, and the sample rod is needed to be provided according to the requirement of the research environment.
The low-temperature freezing technology is widely applied to the scientific fields of physics, chemistry, biology, materials and the like, and according to the observation requirement of an electron microscope in a low-temperature freezing environment, a sample needs to be frozen and fixed before observation, and the process has a direct and important influence on an observation result. If the cooling rate is slow, the aqueous solution in the biological sample will deform and distort the cell structure due to crystallization during the cooling process, resulting in destruction of the structure of the biomolecules.
The rapid freezing technology can enable water to be in a glass state at a low temperature state, reduce the generation of ice crystals, not affect the structure of a sample, and ensure that the sample is observed by an electron microscope at the low temperature state by using a freezing transmission system. But the manufacturers of the domestic quick freezing equipment are few at present, the produced equipment is high in price, the scientific research cost is high, and the scientific research development is not facilitated.
Disclosure of Invention
The application aims to provide a sample freezing and transporting rod, which solves the technical problem that the prior art lacks a freezing device for rapidly achieving a low-temperature observation environment.
In order to solve the technical problems, the application specifically provides the following technical scheme:
the freezing transmission sample rod comprises a rod body and a cold guide rod arranged in the rod body, wherein a rod head for bearing a sample is arranged at the head of the rod body, a refrigeration source loading sleeve cup is arranged at the tail of the rod body, and a first cavity gap is formed between the cold guide rod and the rod body without contact;
the refrigeration source loading sleeve cup comprises an inner cup, an outer cup and an air extraction assembly arranged on the outer cup, wherein the inner cup is arranged in the outer cup and is used for loading liquid nitrogen, the inner cup is not in contact with the outer cup and is provided with a second cavity gap, and the air extraction assembly extends into the outer cup and is communicated with the second cavity gap;
the first cavity gap is communicated with the second cavity gap through the freezing rod seat, the air extraction assembly is used for vacuumizing the first cavity gap and the second cavity gap, liquid nitrogen is added into the inner cup to provide a low-temperature source, and the sample carried in the rod head is cooled through the cooling guide rod under the action of the temperature difference between the rod head side and the inner cup side.
As a preferred aspect of the present application, the rod head includes a freeze transport rod head and a sample carrier ring connected to the freeze transport rod head, the sample carrier ring being used for carrying a sample.
As a preferred scheme of the application, the outer cup comprises an outer cup body and an outer cup cover connected to the top of the outer cup body, the inner cup comprises an inner cup body arranged in the outer cup body, and the inner cup body is not contacted with the outer cup body and forms the second cavity gap;
an inner cup cover is connected to the top of the inner cup body, an inner cup end cover is arranged above the inner part of the inner cup cover, and the inner cup cover is connected with the outer cup cover.
As a preferable scheme of the application, the cold guide rod comprises a long cold rod and a cold guide belt arranged at one side of the end part of the long cold rod, one end of the long cold rod is connected with the freezing transmission rod head, the other end of the long cold rod is connected to the outer wall of the inner cup body through a cold guide stud, and the long cold rod is not contacted with the rod body and forms the first cavity gap.
As a preferred embodiment of the present application, the shaft includes a first freezing shaft near one side of the outer cup and a second freezing shaft connected to the first freezing shaft, an end of the second freezing shaft is connected to the freezing transmission rod head, and an end of the first freezing shaft is connected to the outer cup through a freezing rod seat
As a preferable mode of the application, a temperature sensor is arranged on the outer wall of the long cold rod, the detection end of the temperature sensor is opposite to the first cavity gap between the long cold rod and the rod body, and the temperature sensor is used for detecting the real-time refrigeration temperature of a sample.
As a preferable scheme of the application, an inner cup penetrating pipe is arranged in the inner cup body, a sample transmission protection component is connected to the outer cup body, the sample transmission protection component comprises a transmission protection shaft rod arranged in the long cold rod and a connecting pipe penetrating through the inner cup penetrating pipe, the end part of the long cold rod is connected with the connecting pipe through a cold guide nut, and the connecting pipe is sleeved on the outer side of the transmission protection shaft rod and forms an integral piece.
As a preferable scheme of the application, a drawing seat is connected to the outer wall of the outer cup body, a bidirectional sealing sleeve is arranged in the drawing seat, the connecting pipe is connected with the bidirectional sealing sleeve through a transmission protection connecting nut, and the bidirectional sealing sleeve is sealed with the transmission protection connecting nut through a sealing ring.
As a preferable scheme of the application, the air extraction assembly comprises an air extraction seat, an air extraction pipe and a knob, wherein the air extraction pipe and the knob are connected to the air extraction seat, the air extraction seat is fixedly connected to the outer wall of the outer cup body, an air extraction cavity is arranged in the air extraction seat, the air extraction cavity is in clearance communication with the second cavity, and the air extraction pipe is in clearance communication with the second cavity through the air extraction cavity;
the vacuum pump is characterized in that a sealing shaft penetrating through the air pumping cavity is arranged in the air pumping cavity, the sealing shaft is mounted on the knob, and the knob can drive the sealing shaft to move back and forth through back and forth movement so as to achieve an opening and closing state during vacuumizing.
As a preferred scheme of the application, a transmission protection sleeve and a transmission protection shaft lever are connected to the sample carrying ring, and the transmission protection shaft lever is connected with the transmission protection sleeve through a protection sleeve connecting piece;
the transmission protection axostylus axostyle tip runs through the pull seat, and transmission protection axostylus axostyle tip is provided with transmission protection and draws the cover, transmission protection draws the cover through jackscrew cover with transmission protection axostylus axostyle is connected.
Compared with the prior art, the application has the following beneficial effects:
according to the application, the inner cup is arranged to place liquid nitrogen to provide a refrigeration source, the inner cup body and the outer cup body are combined to provide a vacuum cold guide cavity gap, and the outer cup body is provided with the vacuum drawing component for drawing to form a vacuum environment.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those of ordinary skill in the art that the drawings in the following description are exemplary only and that other implementations can be obtained from the extensions of the drawings provided without inventive effort.
FIG. 1 is a schematic view of a sample rod for freeze transport according to an embodiment of the present application;
FIG. 2 is an exploded view of FIG. 1 provided in an embodiment of the present application;
FIG. 3 is a cross-sectional view of FIG. 1 provided by an embodiment of the present application;
FIG. 4 is a schematic top view of a vacuum area in a working state of a sample rod according to an embodiment of the present application;
FIG. 5 is a schematic diagram illustrating a front cross-sectional view of a vacuum region in a working state of a sample rod according to an embodiment of the present application;
FIG. 6 is a schematic diagram of a sealing shaft on an outer cup in a sealing state when the inside of a sample rod is vacuum cooled;
FIG. 7 is a schematic diagram of a state of a sealing shaft on an outer cup in an open pumping state when the interior of a sample rod is subjected to vacuum cold conduction in the embodiment of the application;
reference numerals in the drawings are respectively as follows:
10-a shaft; 20-a cold guide rod; 30-club head; 40-an inner cup; 50-an outer cup; 60-sample transmission protection assembly;
101-a first frozen shaft; 102-a second frozen shaft; 103-freezing a rod seat; 104-a pin with a seat; 105-freeze shaft pin;
201-long cold bar; 202, a cold guide belt; 203-a cold-conducting nut; 204—a temperature sensor;
301-a freeze transport club head; 302-sample carrier ring;
401-an inner cup; 402-cold-conducting studs; 403-inner cup threading; 404-inner cup cover; 405-inner cup port cover;
501-an outer cup; 502-an outer cup cover; 503-drawing seat; 504-navigation socket; 505-pumping seat; 506-exhaust pipe; 507-sealing the shaft; 508-a knob;
601-a transmission protective sleeve; 602-a protective sleeve connection; 603-transmitting a protection shaft; 604-jackscrew sleeve; 605-transmission protection pull sleeve; 606-connecting the pipes; 607-a transmission protection coupling nut; 608-a bidirectional sealing sleeve.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
As shown in fig. 4 of the specification, the present application provides a sample freezing and transporting rod, comprising a rod body 10 and a cold guide rod 20 arranged in the rod body 10, wherein a rod head 30 for bearing a sample is arranged at the head of the rod body 10, a refrigeration source loading sleeve cup is arranged at the tail of the rod body 10, and a first cavity gap is formed between the cold guide rod 20 and the rod body 10 without contact;
the refrigeration source loading sleeve cup comprises an inner cup 40, an outer cup 50 and an air extraction assembly arranged on the outer cup 50, wherein the inner cup 40 is arranged inside the outer cup 50 and is used for loading liquid nitrogen, the inner cup 40 is not in contact with the outer cup 50 and is provided with a second cavity gap, and the air extraction assembly extends into the outer cup 50 and is communicated with the second cavity gap.
The first cavity gap is communicated with the second cavity gap through the freezing rod seat 103, the air extraction assembly is used for vacuumizing the first cavity gap and the second cavity gap, liquid nitrogen is added into the inner cup 40 to provide a low-temperature source, and the sample carried in the rod head 30 is cooled through the cooling rod 20 under the action of the temperature difference between the rod head 30 side and the inner cup 40 side.
The application is characterized in that the application combines the refrigeration guide cooling and the vacuum environment, so that the sample can be refrigerated in a short time and kept at low temperature for a long time after refrigeration, the efficiency of manufacturing the low-temperature environment is improved, and the stability of the low-temperature vacuum observation environment is improved.
In this embodiment, the rod head 30 includes a freezing transmission rod head 301 and a sample carrying ring 302 disposed on the freezing transmission rod head 301, the sample carrying ring 302 is fixedly connected with the freezing transmission rod head 301 through threads, and the sample carrying ring 302 is used for carrying a sample.
In the embodiment, the outer cup is arranged, so that the inner cup for providing a refrigeration source is combined with a structure for providing a vacuum environment to conduct refrigeration on the sample.
Specifically, the outer cup 50 includes an outer cup 501 and an outer cup cover 502 connected to the top of the outer cup 501, the inner cup 40 includes an inner cup 401 disposed in the outer cup 501 and an inner cup cover 404 connected to the top of the inner cup 401, an inner cup end cover 405 is disposed above the inner cup cover 404, the inner cup cover 404 and the inner cup end cover 405 are connected through threads and are glued, the inner cup cover 404 is welded and fixed on the inner cup 401, the inner cup cover 404 is connected to the outer cup cover 502, and the inner cup 401 is not in contact with the outer cup 501 and forms a second cavity gap.
In this embodiment, the cold guide rod 20 includes a long cold rod 201, a cold guide strip 202 and a cold guide nut 203, the long cold rod 201 is fixedly connected with the cold guide nut 203, and the cold guide strip 202 is fixed between the long cold rod 201 and the cold guide nut 203; one end of the long cold rod 201 is connected with the refrigeration transmission rod head 301, the other end of the long cold rod 201 is connected to the outer wall of the inner cup 401 through a cold guide stud 402, the cold guide stud 402 is welded and fixed on the inner cup 401, and the long cold rod 201 is not contacted with the rod body 10 and forms a first cavity gap.
Wherein, be connected with the subassembly of bleeding on outer cup 501, the subassembly of bleeding stretches into outer cup 501 inside and with second cavity clearance intercommunication, first cavity clearance is through freezing pole seat 103 and second cavity clearance intercommunication, the subassembly of bleeding is used for evacuating first cavity clearance and second cavity clearance into the vacuum environment, add liquid nitrogen in order to provide the low temperature source in the interior cup 401, under the difference in temperature effect between freezing transmission pole head 301 and the interior cup 401, it is cold to lead through cold guide stud 402, cold guide belt 202, cold guide nut 203, long cold pole 201 and freezing transmission pole head 301 in proper order, in order to carry the sample that carries in the sample carrier ring 302 refrigerates.
Also, a temperature sensor 204 is mounted on the outer wall of the long cold bar 201, and the temperature sensor 204 is glued at the outer wall of the long cold bar 201. The detection end of the temperature sensor 204 is opposite to the first cavity gap between the long cold rod 201 and the rod body 10, and is used for detecting the real-time refrigerating temperature of the sample.
Specifically, the air extraction assembly comprises an air extraction seat 505, an air extraction pipe 506 and a knob 508 which are connected to the air extraction seat 505, the air extraction seat 505 is fixedly connected to the outer wall of the outer cup 501, an air extraction cavity is formed in the air extraction seat 505, the air extraction cavity is in clearance communication with the second cavity, and the air extraction pipe 506 is in clearance communication with the second cavity through the air extraction cavity.
And a sealing shaft 507 penetrating through the air extraction cavity is arranged in the air extraction cavity, the sealing shaft 507 is arranged on a knob 508, and the knob 508 can drive the sealing shaft 507 to move back and forth through back and forth movement so as to achieve an opening and closing state during vacuum extraction.
In this embodiment, as shown in fig. 2 and 3, the shaft 10 includes a first freezing shaft 101, a second freezing shaft 102, a freezing shaft seat 103, a seat pin 104, and a freezing shaft pin 105 which are connected, the first freezing shaft 101 near one side of the outer cup 50 connects the second freezing shaft 102 and the freezing shaft seat 103, the end of the second freezing shaft 102 is connected to the freezing transmission club head 301, the end of the first freezing shaft 101 is connected to the outer cup 501 through the freezing shaft seat 103, the seat pin 104 is fixedly connected to the freezing shaft seat 103, the seat pin 104 is used for positioning when the transmission electron microscope is installed in the electron microscope, the freezing shaft pin 105 is welded and fixed on the second freezing shaft 102, and the freezing shaft pin 105 is used for positioning when the transmission electron microscope is installed in the electron microscope; the sealing ring M2 is arranged in the middle of the first freezing rod body 101 and is used for sealing when the transmission electron microscope freezes and transmits the sample rod to be installed in the electron microscope.
In addition, the embodiment is further provided with a protection component for protecting the sample in the transferring process, specifically, an inner cup penetrating pipe 403 is arranged in the inner cup 401, the inner cup penetrating pipe 403 is welded and fixed on the inner cup 401, the outer cup 501 is connected with a sample transmission protection component 60 through a drawing seat 503 and a navigation socket 504, and the drawing seat 503 and the navigation socket 504 are respectively and fixedly connected on the outer cup 501.
In this embodiment, the sample transmission protection assembly 60 includes a transmission protection sleeve 601, a protection sleeve connector 602, a transmission protection shaft 603, a jackscrew sleeve 604, a transmission protection sleeve 605, a connection pipe 606, a transmission protection connection nut 607, and a bidirectional sealing sleeve 608, and the transmission protection sleeve 601 can circumferentially protect the sample after pushing the transmission protection sleeve 605.
The transmission protection shaft rod 603 is arranged inside the long cooling rod 201, the connecting pipe 606 is arranged inside the inner cup penetrating pipe 403 in a penetrating way, the end part of the long cooling rod 201 is connected with the connecting pipe 606 through the cold guide nut 203, and the bidirectional sealing sleeve 608 is arranged in the drawing seat 503; the transmission protection sleeve 601 is connected to the sample carrier ring 302, and the transmission protection shaft 603 is connected to the transmission protection sleeve 601 through the protection sleeve connecting piece 602; the jackscrew sleeve 604 fixedly connects the transmission protection pull sleeve 605 with the transmission protection shaft 603, the transmission protection connecting nut 607 seals the connecting pipe 606 with the bidirectional sealing sleeve 608, and the bidirectional sealing sleeve 608 is sealed with the transmission protection connecting nut 607 by using the sealing ring M4.
In this embodiment, the sample transmission protection assembly 60 passes through the drawing seat 503 of the outer cup 50, and the space between the sample transmission protection assembly 60 and the outer cup 50 is sealed by using a sealing ring M5. The shaft 10 and the outer cup 50 are sealed with a gasket M3.
In summary, in the above embodiments
The cold-conducting structure comprises: the inner cup 401, cold guide stud 402, cold guide nut 203, long cold bar 201, cold transfer head 301, and sample carrier ring 302 are sequentially connected in sequence, conducting temperature by direct contact.
The principle of cold conduction is as follows: liquid nitrogen is poured into the inner cup 401, and the temperature of the sample carrying ring 302 is reduced by the inner cup 401 sequentially passing through the cold guide stud 402, the cold guide belt 202, the cold guide nut 203, the long cold rod 201 and the freezing transmission rod head 301.
The heat preservation principle is as follows: the outer cup 501 wrapped outside the inner cup 401 and the shaft 10 wrapped outside the long cold bar 201 should function as a heat insulating layer, and since temperature is difficult to be conducted through a vacuum environment, the heat insulating function is achieved by performing a vacuum process on a first cavity gap between the long cold bar 201 and the shaft 10 and a second cavity gap between the inner cup 401 and the outer cup 501.
Working principle: the sample is placed on the rod head 30 of a frozen transmission sample rod, and before the frozen transmission sample rod is installed in a transmission electron microscope, the transmission protection sleeve 601 can be moved forward by pushing the transmission protection sleeve 605, so that the sample can be moderately protected under the external environment state. After the transmission electron microscope is frozen and the transmission sample rod is installed in the transmission electron microscope, the transmission protection pull sleeve 605 is pulled backwards, so that the transmission protection sleeve 601 moves the sample backwards to reach an observable state.
In a transmission electron microscope, after the transmission electron microscope is assembled, the sealing shaft 507 is operated to an open state by an operation knob 508 as shown in fig. 6 and 7, and a vacuum pumping operation is performed by an air pumping pipe 506 as shown in fig. 5, so that a vacuum environment is formed between the rod body 10 and the cold guide rod 20, and between the inner cup 40 and the outer cup 50, and the vacuum state is formed, as shown in fig. 4, and the operation can improve the refrigerating efficiency and the maintenance time after the temperature reduction.
After the preparation is completed, liquid nitrogen is added into the inner cup 40, and a low temperature environment is formed in the inner cup 40 by the following sequence: the inner cup 401, the cold guide stud 402, the cold guide belt 202, the long cold rod 201, the freezing transmission rod head 301, and the sample carrier ring 302 are used for guiding cold to a sample. The optimized process time can be controlled to be less than 35min.
After standing for unit time, the sample rod for the freezing transmission of the latter type can be optimized to provide a low-temperature environment which is better than that of more than 5 hours. The temperature value may be monitored in real time by the temperature sensor 204 during this time period.
The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application, the scope of which is defined by the claims. Various modifications and equivalent arrangements of this application will occur to those skilled in the art, and are intended to be within the spirit and scope of the application.
Claims (10)
1. The freezing transmission sample rod is characterized by comprising a rod body (10) and a cold guide rod (20) arranged in the rod body (10), wherein a rod head (30) for bearing a sample is arranged at the head of the rod body (10), a refrigerating source loading sleeve cup is arranged at the tail of the rod body (10), and a first cavity gap is formed between the cold guide rod (20) and the rod body (10) without contact;
the refrigeration source loading sleeve cup comprises an inner cup (40), an outer cup (50) and an air extraction assembly arranged on the outer cup (50), wherein the inner cup (40) is arranged inside the outer cup (50) and is used for loading liquid nitrogen, the inner cup (40) is not contacted with the outer cup (50) and is formed with a second cavity gap, and the air extraction assembly extends into the outer cup (50) and is communicated with the second cavity gap;
the first cavity gap is communicated with the second cavity gap through a freezing rod seat (103), the air extraction assembly is used for vacuumizing the first cavity gap and the second cavity gap, liquid nitrogen is added into the inner cup (40) to provide a low-temperature source, and the sample carried in the rod head (30) is cooled through the cooling rod (20) under the action of the temperature difference between the rod head (30) side and the inner cup (40) side.
2. A freeze transport sample rod according to claim 1, characterized in that the rod head (30) comprises a freeze transport rod head (301) and a sample carrier ring (302) connected to the freeze transport rod head (301), the sample carrier ring (302) being adapted to carry a sample.
3. A frozen transmission sample rod according to claim 2, characterized in that the outer cup (50) comprises an outer cup (501) and an outer cup cover (502) connected to the top of the outer cup (501), the inner cup (40) comprises an inner cup (401) arranged inside the outer cup (501), and the inner cup (401) is not in contact with the outer cup (501) and forms the second cavity gap;
an inner cup cover (404) is connected to the top of the inner cup body (401), an inner cup end cover (405) is arranged above the inner cup cover (404), and the inner cup cover (404) is connected with the outer cup cover (502).
4. A freeze transport sample rod according to claim 3, characterized in that the cold guide rod (20) comprises a long cold rod (201) and a cold guide belt (202) arranged on one side of the end of the long cold rod (201), one end of the long cold rod (201) is connected with the freeze transport rod head (301), the other end of the long cold rod (201) is connected on the outer wall of the inner cup body (401) through a cold guide stud (402), and the long cold rod (201) is not contacted with the rod body (10) and forms the first cavity gap.
5. The frozen transmission sample rod according to claim 4, characterized in that the rod body (10) comprises a first frozen rod body (101) close to one side of the outer cup (50) and a second frozen rod body (102) connected to the first frozen rod body (101), wherein the end part of the second frozen rod body (102) is connected with the frozen transmission rod head (301), and the end part of the first frozen rod body (101) is connected to the outer cup body (501) through a frozen rod seat (103).
6. A freeze transport sample rod according to claim 4, characterized in that a temperature sensor (204) is mounted on the outer wall of the long cold rod (201), the detection end of the temperature sensor (204) is directly opposite to the first cavity gap between the long cold rod (201) and the rod body (10) and is used for detecting the real-time refrigeration temperature of the sample.
7. The frozen transmission sample rod according to claim 4, characterized in that an inner cup through pipe (403) is arranged in the inner cup (401), a sample transmission protection assembly (60) is connected to the outer cup (501), the sample transmission protection assembly (60) comprises a transmission protection shaft rod (603) installed inside the long cold rod (201) and a connecting pipe (606) installed inside the inner cup through pipe (403) in a penetrating way, the end part of the long cold rod (201) is connected with the connecting pipe (606) through a cold guide nut (203), and the connecting pipe (606) is sleeved outside the transmission protection shaft rod (603) and forms an integral piece.
8. The frozen transmission sample rod according to claim 7, characterized in that a drawing seat (503) is connected to the outer wall of the outer cup body (501), a bidirectional sealing sleeve (608) is arranged in the drawing seat (503), the connecting pipe (606) is connected with the bidirectional sealing sleeve (608) through a transmission protection connecting nut (607), and the bidirectional sealing sleeve (608) is sealed with the transmission protection connecting nut (607) through a sealing ring (M4).
9. A frozen transmission sample rod according to claim 3, characterized in that the air extraction assembly comprises an air extraction seat (505) and an air extraction tube (506) and a knob (508) which are connected to the air extraction seat (505), the air extraction seat (505) is fixedly connected to the outer wall of the outer cup body (501), an air extraction cavity is arranged inside the air extraction seat (505), the air extraction cavity is communicated with the second cavity gap, and the air extraction tube (506) is communicated with the second cavity gap through the air extraction cavity;
the sealing shaft (507) penetrating through the air extraction cavity is arranged in the air extraction cavity, the sealing shaft (507) is arranged on the knob (508), and the knob (508) can drive the sealing shaft (507) to move back and forth through back and forth movement so as to achieve an opening and closing state when vacuumizing.
10. A frozen transmission sample rod according to claim 8, characterized in that a transmission protection sleeve (601) and a transmission protection shaft (603) are connected to the sample carrier ring (302), the transmission protection shaft (603) being connected to the transmission protection sleeve (601) by a protection sleeve connection (602);
the end part of the transmission protection shaft rod (603) penetrates through the drawing seat (503), a transmission protection drawing sleeve (605) is arranged at the end part of the transmission protection shaft rod (603), and the transmission protection drawing sleeve (605) is connected with the transmission protection shaft rod (603) through a jackscrew sleeve (604).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310533307.7A CN116682715A (en) | 2023-05-12 | 2023-05-12 | Freezing transmission sample pole |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310533307.7A CN116682715A (en) | 2023-05-12 | 2023-05-12 | Freezing transmission sample pole |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116682715A true CN116682715A (en) | 2023-09-01 |
Family
ID=87786255
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310533307.7A Pending CN116682715A (en) | 2023-05-12 | 2023-05-12 | Freezing transmission sample pole |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116682715A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118280795A (en) * | 2024-06-03 | 2024-07-02 | 海尔生物医疗科技(成都)有限公司 | Sample low-temperature transmission system for refrigeration electron microscope |
-
2023
- 2023-05-12 CN CN202310533307.7A patent/CN116682715A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118280795A (en) * | 2024-06-03 | 2024-07-02 | 海尔生物医疗科技(成都)有限公司 | Sample low-temperature transmission system for refrigeration electron microscope |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN116682715A (en) | Freezing transmission sample pole | |
| KR101066418B1 (en) | Improved sample pre-treatment unit in a low temperature sample concentration system of gas phase compounds | |
| CN208504804U (en) | A kind of ultralow temperature condensation enrichment system | |
| CN102669088B (en) | Mammal embryo and oocyte vitrification freezing carrier | |
| CN107817067B (en) | Large-cavity high-low temperature loading device for neutron stress measurement | |
| CN100439817C (en) | Electron optical device for direct cooling long wave infrared detector of pulse tube refrigerator | |
| CN208283171U (en) | A kind of combination detachable Material Testing Machine cryogenic freezing case | |
| CN114324429B (en) | Sample freezing and transmission integrated device for scanning electron microscope | |
| JP3374273B2 (en) | High magnetic field low temperature physical property measurement device | |
| CN109360707A (en) | Plug-in current down-lead structure and superconducting magnet | |
| CN218239542U (en) | Novel biochemical analyzer refrigeration device | |
| CN112179750B (en) | Enrichment apparatus and method | |
| CN219020054U (en) | Tumor cell liquid preservation test tube | |
| CN210119055U (en) | Cabinet is stored to clinical laboratory's sample | |
| CN209399604U (en) | A kind of refrigeration detecting structure of atmosphere nitrogen-oxide analyzer, photomultiplier tube | |
| CN218752152U (en) | Temporary ward specimen storage device for molecular joint detection | |
| CN108548693B (en) | Vacuum self-cooling sampler | |
| CN204473525U (en) | One is special freezes tank | |
| CN221661495U (en) | Freezing box assembly capable of maintaining short-time low temperature | |
| CN210960121U (en) | Micro-evaporation supercooled liquid nitrogen biological sample storage tank | |
| US6810679B2 (en) | Cooling apparatus and squid microscope using same | |
| CN209214178U (en) | A kind of quickly cooling heat preservation can body | |
| CN212299608U (en) | Ultra-low temperature preservation box | |
| CN210913690U (en) | Dry-wet probiotics storage box | |
| CN214357499U (en) | Ultra-low temperature cryopreserved box easy to open |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |