CN116679061B - Marine Pythium detection kit - Google Patents

Marine Pythium detection kit Download PDF

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Publication number
CN116679061B
CN116679061B CN202310966591.7A CN202310966591A CN116679061B CN 116679061 B CN116679061 B CN 116679061B CN 202310966591 A CN202310966591 A CN 202310966591A CN 116679061 B CN116679061 B CN 116679061B
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conductive
bobbin
spring
box body
pythium
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CN116679061A (en
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许华
李贵阳
徐东
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
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  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention provides a marine Pythium detection kit, which comprises a box body, wherein a rotary table is arranged in the box body through a rotary column, a plurality of mounting openings are formed in the circumferential side wall of the rotary table, a pair of reversible supporting plates are arranged in the mounting openings, a rotating ring capable of sliding vertically is elastically arranged between the supporting plates, a bobbin is rotatably connected in the rotating ring through a one-way bearing, the top of the bobbin is arranged in a closed manner, an annular knife edge is arranged at the bottom of the bobbin, an opening and closing mechanism is further arranged at the bottom of the bobbin, the opening and closing mechanism comprises an opening and closing plate for closing the bobbin, and a liquid outlet hole is formed in the opening and closing plate. The kit is convenient to carry, can be directly used in an outer belt, does not need to be detected in a laboratory, can automatically collect tissues of different marine algae vegetables, automatically completes the injection, oscillation and centrifugation processes of diluent, and finally automatically extrudes and drops the liquid to be detected onto a detection card, thereby rapidly obtaining a detection result.

Description

Marine Pythium detection kit
Technical Field
The invention relates to the technical field of Pythium detection, in particular to a marine Pythium detection kit.
Background
The marine algae vegetables comprise thallus Porphyrae, thallus Gracilariae, undaria pinnatifida, cyrtymenia Sparsa, ma Nizao, herba Zosterae Marinae, etc., and are rich in high-quality proteins, amino acids, vitamins, and minerals such as phosphorus, magnesium, sodium, potassium, calcium, iodine, iron, silicon, manganese, and zinc essential for human body, wherein some components are not available in terrestrial vegetables. Algae vegetables often develop Pythium fungi during the processing and storage stages.
The patent with publication number CN105316427A relates to a fluorescent quantitative PCR detection kit for rapidly detecting the pythium aphanidermatum, and discloses a rapid detection mode which adopts a detection card at present, wherein PCR reaction is carried out by taking the pythium aphanidermatum DNA as a template, and specific primers GF1F/GF1R designed in an ITS zone of the pythium aphanidermatum rDNA are amplified to obtain 282bp products through specific amplification.
However, most of the two detection means for the Pythium species require that detection personnel firstly take samples on site, cut off partial tissues from various algae and vegetables by using a cutter, then put the partial tissues into a sampling bottle and send the samples into a laboratory for detection, the detection process in the laboratory requires multiple reagents and instruments for cooperation, and after the samples are subjected to multi-step treatment, the samples are dripped into a special detection card to obtain detection results, so that the detection results are more troublesome in both sampling step and detection step, more manual operation flows and low detection speed.
Disclosure of Invention
Aiming at the problems, the invention provides the marine Pythium detection kit which is convenient to carry, can be directly taken out for use without detection in a laboratory, can automatically collect leaf tissues of different vegetables and the like, automatically completes the injection, oscillation and centrifugation processes of diluent, and finally automatically extrudes and drops the liquid to be detected onto a detection card to quickly obtain a detection result.
In order to solve the problems, the invention adopts the following technical scheme:
the utility model provides a marine Pythium detection kit, includes the box body, install the carousel through the column spinner in the box body, the circumference lateral wall of carousel is equipped with a plurality of installing ports, a plurality of install the downthehole a pair of backup pad that can overturn of installing, a pair of elastic mounting can vertical slip swivel between the backup pad, rotate through the one-way bearing in the swivel and be connected with the bobbin, the top closure setting of bobbin, the bottom is equipped with annular edge of a knife, and closing mechanism is still installed to the bottom of bobbin, closing mechanism includes the opening and closing plate that is used for closed bobbin, be equipped with out the liquid hole on the opening and closing plate, the extrusion mechanism that can extrude out the liquid to be measured from out the liquid hole is still installed at the top of bobbin;
the front side of box body is the sample position, and the right side is smashing the position, and the rear side is the oscillation position, and the left side is for surveying the position, be annotating the liquid level between smashing position and the oscillation position, survey and be centrifugal position between position and the oscillation position, the interior top of box body is located sample position department and installs electric telescopic handle, electric telescopic handle can drive the bobbin of corresponding position and reciprocate, the bottom of box body is located centrifugal position department and installs and to drive bobbin pivoted motor.
Preferably, a door body is arranged at the front side of the box body corresponding to the sampling position, and an inserting port is arranged at the left side of the box body corresponding to the measuring position.
Preferably, both sides of the mounting port are provided with bar grooves, racks are elastically connected in the bar grooves through first springs, rotating shafts are rotatably connected in the bar grooves, the tail ends of the rotating shafts are fixedly connected with corresponding side support plates, and gears meshed with the racks are fixed on the rotating shafts.
Preferably, the opening and closing mechanism further comprises a push-pull box fixedly connected to the side wall of the bottom of the bobbin, and a second spring elastically connected with the opening and closing plate is arranged in the push-pull box.
Preferably, a sliding groove is formed in the side wall of the supporting plate, a sliding block is elastically connected in the sliding groove through a third spring, and the side wall of the sliding block is fixedly connected with the swivel.
Preferably, the extrusion mechanism comprises a cylinder fixedly connected to the top of the bobbin, a supporting rod is elastically connected to the cylinder through a fourth spring, the lower end of the supporting rod extends into the bobbin and is fixedly connected with a piston, and the top of the cylinder and the output end of the motor are both fixedly provided with matched magnetic blocks.
Preferably, the electric power supply device further comprises a power supply mechanism capable of supplying power to the first spring, the second spring, the third spring and the fourth spring, the power supply mechanism comprises a conductive component arranged on the rotary table and a power supply component arranged at the inner top of the box body, the conductive component comprises a plurality of groups of conductive components which are arranged at the top of the rotary table and correspond to the bobbins one by one, the groups of conductive components consist of a first conductive head, a second conductive head, a third conductive head and a fourth conductive head which are arranged from outside to inside in radial direction, the first conductive head is electrically connected with the first spring, the second conductive head is electrically connected with the second spring, the third conductive head is electrically connected with the third spring, and the fourth conductive head is electrically connected with the fourth spring.
Preferably, the power supply assembly comprises a mounting column fixedly connected to the inner top of the box body, a first conductive rail matched with a first conductive head is mounted at the bottom of the mounting column, a second conductive rail matched with a second conductive head, a first conductive seat matched with a third conductive head and two second conductive seats matched with a fourth conductive head are mounted at the bottom of the mounting column, the first conductive rail circumferentially extends from a crushing position to a centrifugal position, the second conductive rail circumferentially extends from an oversampling position to an injection liquid level, the first conductive seats correspond to an oscillating position and are externally connected with a pulse switch, one second conductive seat is located between the centrifugal position and a measuring position, and the other second conductive seat is located at the measuring position.
Preferably, the top of box body corresponds crushing position department and is equipped with the mouth of placing, place the mouth and be used for inserting crushing apparatus, the top of box body corresponds annotates liquid level department and is equipped with annotates the liquid pipe.
Preferably, pressure membrane flaps are arranged at two ends of the liquid outlet hole.
The beneficial effects of the invention are as follows:
(1) Through setting up the sample position, smashing the position, annotating liquid level, vibration position, centrifugation position and survey position, adopt the form of carousel, smash the sample in proper order, automatic annotate liquid, vibration and centrifugation process, turn to the position and detect the process afterwards, the box body can directly take the use outward, need not to accomplish the detection process in the laboratory.
(2) The door body is opened through the installation bobbin, the third spring and the electric telescopic rod, the algae vegetables to be detected are placed at the sampling position at the bottom of the box body, the electric telescopic rod is started to stretch back and forth, the bobbin moves up and down in a reciprocating mode under the reset effect of the third spring, the bobbin moves downwards to cut the algae vegetables through the annular knife edge at the lower end, the algae vegetables can be applied to various algae vegetables, the cut algae vegetable tissues are plugged into the bobbin, and the sampling sufficiency is guaranteed through multiple cutting.
(3) Through installing first spring, rack, gear, pivot and power supply mechanism, after accomplishing the cutting sample, the carousel rotates, before the sample changes to smashing the position, first conductive track of first spring circular telegram shrink pulling rack, and then drive the bobbin upset 180 through gear and pivot, can all keep the nozzle state up at smashing, annotate liquid and centrifugation process, in being convenient for insert the bobbin with crushing apparatus's tool bit from the mouth of placing at box body top, also be convenient for annotate the liquid pipe and pour into the bobbin with the diluent into.
(4) Through installing closing mechanism, at sample, smash and annotate the liquid in-process, no. two conductive heads contact the second conductor rail all the time, and then the second spring remains the switch-on state all the time, and then the switching board is in push-and-pull box all the time, and the nozzle remains open state, and when turning to oscillation position, centrifugation position and survey location, no. two conductive heads break away from the second conductor rail, and the second spring outage resets, and the closed nozzle of switching door avoids oscillation, centrifugation and liquid leakage during the survey.
(5) And after the injected diluent is turned to the oscillation position, the third conducting head is contacted with the first conducting seat, the pulse switch is intermittently started, the third spring is further intermittently electrified, and the bobbin is driven to shake up and down through the sliding block and the swivel, so that the oscillation process is automatically completed.
(6) Through installing motor and magnetic path, turn to the centrifugation position after, under the magnetic attraction effect of a pair of magnetic path, the motor output shaft is in the barrel butt joint of lower extreme this moment, and the starter motor can drive the bobbin and rotate fast, accomplishes centrifugal process, through setting up one-way bearing, when using crushing equipment to insert the bobbin and smash, smash the rotation direction of tool bit and one-way bearing rotation direction opposite, and then can not lead to the bobbin to rotate simultaneously.
(7) Through installation extrusion mechanism, when the second conductive seat between fourth conductive head contact centrifugation position and the survey position, the nozzle of bobbin is in the top this moment, fourth spring circular telegram shrink, promote the piston and reciprocate, extrude the supernatant after the centrifugation promptly to the play downthehole, the extrusion force can make first pressure membrane lamella open, the second pressure membrane lamella is because the pressure is not in closed state, after the position is surveyed to the spool, the nozzle is down in the upset again, the second conductive seat of fourth conductive head contact survey position department this moment, the piston secondary removes, the distance of secondary removal is greater than first time, and then the pressure is enough to dash out second pressure membrane lamella, the liquid that awaits measuring in the play downthehole extrusion drip of liquid is on the detection card, accomplish automated inspection process.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a cross-sectional view of a turntable according to the present invention;
FIG. 3 is an enlarged schematic view of the structure at A in FIG. 2;
FIG. 4 is a cross-sectional view of a bobbin in accordance with the present invention;
FIG. 5 is a cross-sectional view of a push-pull box according to the present invention;
FIG. 6 is a top view of the inside of the case according to the present invention;
fig. 7 is a top view of the bottom of the case according to the present invention.
In the figure: 1. a case body; 2. a turntable; 3. an insertion port; 4. a door body; 5. a mounting port; 6. a spin column; 7. a bobbin; 8. a push-pull box; 9. a first conductive head; 10. a second conductive head; 11. a third conductive head; 12. a fourth conductive head; 13. a swivel; 14. a cylinder; 15. a bar-shaped groove; 16. a one-way bearing; 17. a support plate; 18. a slide block; 19. a rack; 20. a gear; 21. a rotating shaft; 22. a first spring; 23. a magnetic block; 24. a fourth spring; 25. a supporting rod; 26. a piston; 27. an annular knife edge; 28. a pressure membrane flap; 29. an opening/closing plate; 30. a liquid outlet hole; 31. a third spring; 32. a chute; 33. a motor; 34. a second spring; 35. a liquid injection pipe; 36. a placement port; 37. an electric telescopic rod; 38. a mounting column; 39. a first conductor rail; 40. a second conductor rail; 41. a first conductive base; 42. and a second conductive seat.
Detailed Description
In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit or scope of the invention, which is therefore not limited to the specific embodiments disclosed below.
It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.
Referring to fig. 1-7, a marine pythium fungus detection kit comprises a box body 1, a turntable 2 is installed in the box body 1 through a rotary column 6, a plurality of mounting openings 5 are formed in the circumferential side wall of the turntable 2, a pair of turnover supporting plates 17 are installed in the plurality of mounting openings 5, strip grooves 15 are formed in two sides of each mounting opening 5, racks 19 are elastically connected in the strip grooves 15 through first springs 22, a rotating shaft 21 is rotationally connected in the strip grooves 15, the tail ends of the rotating shaft 21 are fixedly connected with the corresponding side supporting plates 17, a gear 20 meshed with the racks 19 is fixed on the rotating shaft 21, a first conductive head 9 contacts with the first conductive rail 39 before a sample is turned to a crushing position, the first springs 22 at the corresponding positions are electrified to shrink and pull the racks 19, and then the spool 7 is driven to turn 180 degrees through the gears 20 and the rotating shaft 21, so that the spool 7 is turned upwards after the sample is turned to a measuring position, the spool 7 is reset to turn downwards again due to the first conductive head 9 being separated from the first conductive rail 39.
A rotating ring 13 capable of sliding vertically is elastically installed between a pair of supporting plates 17, sliding grooves 32 are formed in the side walls of the supporting plates 17, sliding blocks 18 are elastically connected in the sliding grooves 32 through third springs 31, the side walls of the sliding blocks 18 are fixedly connected with the rotating ring 13, after diluent is injected and rotates to an oscillation position, third conducting heads 11 are in contact with first conducting seats 41, pulse switches are intermittently started, then the third springs 31 are intermittently electrified, the sliding blocks 18 and the rotating ring 13 drive bobbins 7 to shake up and down, and an oscillation process is automatically completed.
The bobbin 7 is rotatably connected in the swivel 13 through the one-way bearing 16, the top of the bobbin 7 is closed, the bottom is provided with the annular knife edge 27, the bottom of the bobbin 7 is also provided with an opening and closing mechanism, the opening and closing mechanism comprises an opening and closing plate 29 for closing the bobbin 7, the opening and closing mechanism also comprises a push-pull box 8 fixedly connected to the side wall of the bottom of the bobbin 7, a second spring 34 elastically connected with the opening and closing plate 29 is arranged in the push-pull box 8, the second conducting head 10 always contacts the second conducting rail 40 in the processes of sampling, crushing and liquid injection, the second spring 34 always keeps in an electrified state, the opening and closing plate 29 is always in the push-pull box 8, the opening is kept in an open state, when the bobbin is rotated to the oscillating position, the centrifugal position and the measuring position, the second conducting head 10 is separated from the second conducting rail 40, the second spring 34 is in the outage reset, the opening and closing of the opening of the bobbin, and liquid leakage during oscillation, the centrifugal position and measuring are avoided.
Further, two conductive rings can be embedded in the swivel 13, two brushes abutting against the conductive rings are fixed on the side wall of the bobbin 7, the two brushes can be electrically connected with the second spring 34 and the fourth spring 24 respectively, and the two conductive rings are electrically connected with the fourth conductive head 12 and the second conductive head 10 respectively, so as to ensure the energizing state in the rotating process.
The opening and closing plate 29 is provided with a liquid outlet hole 30, the top of the bobbin 7 is also provided with an extrusion mechanism capable of extruding liquid to be detected from the liquid outlet hole 30, the extrusion mechanism comprises a cylinder 14 fixedly connected to the top of the bobbin 7, a supporting rod 25 is elastically connected to the cylinder 14 through a fourth spring 24, the lower end of the supporting rod 25 extends into the bobbin 7 and is fixedly connected with a piston 26, and the centrifuged supernatant can be extruded and dropped on the detection card through twice extrusion.
The front side of the box body 1 is a sampling position, the right side is a crushing position, the rear side is an oscillating position, the left side is a measuring position, a liquid filling level is arranged between the crushing position and the oscillating position, a centrifugal position is arranged between the measuring position and the oscillating position, an electric telescopic rod 37 is arranged at the position of the sampling position on the inner top of the box body 1, the electric telescopic rod 37 can drive a bobbin 7 at a corresponding position to move up and down, the electric telescopic rod 37 is started to reciprocate, and meanwhile, under the reset action of a third spring 31, the bobbin 7 moves up and down in a reciprocating manner, and the bobbin 7 moves down to cut algae and vegetables through an annular knife edge 27 at the lower end.
The bottom of the box body 1 is provided with a motor 33 which is positioned at the centrifugal position and can drive the bobbin 7 to rotate, the top of the barrel 14 and the output end of the motor 33 are both fixed with magnetic blocks 23 which are matched, the box body is rotated to the centrifugal position after being oscillated, under the action of the magnetic attraction force of a pair of magnetic blocks 23, the output shaft of the motor 33 is magnetically attracted and butted with the barrel 14 which is positioned at the lower end at the moment, and the motor 33 is started to drive the bobbin 7 to rotate rapidly, so that the centrifugal process is completed.
In order to realize the sampling, oscillating, centrifuging and measuring processes, the device further comprises a power supply mechanism capable of electrifying the first spring 22, the second spring 34, the third spring 31 and the fourth spring 24, wherein the power supply mechanism comprises a conductive component arranged on the rotary table 2 and a power supply component arranged at the inner top of the box body 1, the conductive component comprises a plurality of groups of conductive components which are arranged at the top of the rotary table 2 and correspond to the bobbins 7 one by one, the groups of conductive components are composed of a first conductive head 9, a second conductive head 10, a third conductive head 11 and a fourth conductive head 12 which are arranged from outside to inside in radial directions, the first conductive head 9 is electrically connected with the first spring 22, the second conductive head 10 is electrically connected with the second spring 34, the third conductive head 11 is electrically connected with the third spring 31, and the fourth conductive head 12 is electrically connected with the fourth spring 24.
Further, the power supply assembly comprises a mounting column 38 fixedly connected to the inner top of the box body 1, a first conductive rail 39 matched with the first conductive head 9, a second conductive rail 40 matched with the second conductive head 10, a first conductive seat 41 matched with the third conductive head 11, two second conductive seats 42 matched with the fourth conductive head 12, the first conductive rail 39 circumferentially extends from the crushing position to the centrifugal position, the second conductive rail 40 circumferentially extends from the over-sampling position to the filling liquid level, the first conductive seat 41 corresponds to the oscillating position and is externally connected with a pulse switch, one second conductive seat 42 is located between the centrifugal position and the measuring position, and the other second conductive seat 42 is located in the measuring position.
Furthermore, a door body 4 is installed at the front side of the box body 1 corresponding to the sampling position, an insertion opening 3 is arranged at the left side of the box body 1 corresponding to the measuring position, and the detecting card can be inserted into the measuring position from the insertion opening 3.
Further, the top of box body 1 corresponds crushing position department and is equipped with places mouth 36, places mouthful 36 and is used for inserting crushing apparatus, can be artificial insert the tool bit of crushing apparatus in the bobbin 7 through the mouth 36 of placing at box body 1 top, smashes the sample of sampling, takes out crushing apparatus after smashing, washes crushing tool bit for next use, and the top of box body 1 corresponds annotates liquid level department and is equipped with annotates liquid pipe 35, annotates intraductal with diluent injection through annotating liquid pipe 35, annotates liquid pipe 35 external water pump and liquid storage pot.
During sampling, the door body 4 is opened, the algae vegetables to be detected are placed at the sampling position at the bottom of the box body 1, the electric telescopic rod 37 is started to reciprocate and stretch, meanwhile, under the reset action of the third spring 31, the bobbin 7 moves up and down to reciprocate, the bobbin 7 moves down to cut the algae vegetables through the annular knife edge 27 at the lower end, the algae vegetables can be suitable for various algae vegetables, the cut algae vegetable tissues are plugged into the bobbin 7, and the bobbin 7 is filled with sufficient samples after the algae vegetables are cut for many times.
When the cutting sampling is completed, the rotary column 6 drives the rotary table 2 to rotate, before the sample rotates to the crushing position, the first conductive head 9 contacts the first conductive rail 39, the first spring 22 at the corresponding position is electrified to shrink to pull the rack 19, and then the bobbin 7 is driven to turn 180 degrees through the gear 20 and the rotary shaft 21, so that the nozzle is upward, as the first conductive rail 39 circumferentially extends from the crushing position to the centrifugal position, the nozzle is kept upward in the crushing, liquid injection and centrifugal processes, after the rotary column rotates to the crushing position, the cutter head of the crushing device can be manually inserted into the bobbin 7 through the placing opening 36 at the top of the box body 1, the sampled sample is crushed, the crushing device is taken out after crushing, the cutter head is washed for the next use, the rotary column is continuously rotated to the liquid injection level, the diluent is injected into the bobbin 7 through the liquid injection pipe 35, and the liquid injection pipe 35 is externally connected with the water pump and the liquid storage tank.
In the sampling, crushing and liquid injection processes, the second conductive head 10 always contacts with the second conductive rail 40, then the second spring 34 always keeps in an electrified state, and then the opening and closing plate 29 is always positioned in the push-pull box 8, the nozzle keeps in an open state, when the second conductive head 10 is turned to an oscillation position, a centrifugal position and a measurement position, the second conductive head 10 is separated from the second conductive rail 40, the second spring 34 is powered off and reset, and the opening and closing plate 29 closes the nozzle to avoid liquid leakage during oscillation, centrifugation and measurement.
After the injected diluent rotates to the oscillation position, the third conductive head 11 contacts the first conductive seat 41, the pulse switch is intermittently started, the third spring 31 is intermittently electrified, the bobbin 7 is driven to shake up and down by the sliding block 18 and the swivel 13, and the oscillation process is automatically completed.
After oscillation, the rotor is rotated to a centrifugal position, under the action of the magnetic attraction force of the pair of magnetic blocks 23, the output shaft of the motor is magnetically attracted to the cylinder 14 at the lower end at the moment, the motor 33 is started to drive the bobbin 7 to rotate rapidly, the centrifugal process is completed, and it is worth noting that by arranging the one-way bearing 16, when the crushing equipment is inserted into the bobbin 7 for crushing, the rotation direction of the crushing cutter head is opposite to that of the one-way bearing 16, and the bobbin 7 is not caused to rotate simultaneously.
When the fourth conductive head 12 contacts the second conductive seat 42 between the centrifugal position and the measuring position, the barrel opening of the barrel 7 is positioned above, the fourth spring 24 is electrified and contracted to push the piston 26 to move upwards, the centrifuged supernatant, namely the liquid to be measured, is extruded into the liquid outlet 30, the extrusion force can enable the first pressure membrane valve 28 to be opened, the second pressure membrane valve 28 is in a closed state due to insufficient pressure, after the barrel 7 is turned to the measuring position, the first conductive head 9 is separated from the first conductive rail 39 to reset and turn over again, so that the barrel opening faces downwards, at the moment, the fourth conductive head 12 contacts the second conductive seat 42 at the measuring position, the piston 26 moves for the second time, the distance of the second movement is larger than that of the first time, the pressure is enough to blow the second pressure membrane valve 28, and the liquid to be measured in the liquid outlet 30 is extruded and dropped on the detecting card, and the automatic detection process is completed.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims (10)

1. The utility model provides a marine Pythium detection kit, includes box body (1), its characterized in that, install carousel (2) through column spinner (6) in box body (1), the circumference lateral wall of carousel (2) is equipped with a plurality of installing ports (5), a plurality of install in all installing ports (5) a pair of backup pad (17) that can overturn, a pair of elastic mounting has swivel (13) that can vertically slide between backup pad (17), rotate through one-way bearing (16) in swivel (13) and be connected with bobbin (7), the top of bobbin (7) is closed to be set up, and the bottom is equipped with annular knife edge (27), and closing mechanism is still installed to the bottom of bobbin (7), closing mechanism is including opening and closing plate (29) that are used for closed bobbin (7), be equipped with liquid outlet (30) on opening and closing plate (29), the extrusion mechanism that can extrude liquid to be measured from liquid outlet (30) is still installed at the top of bobbin (7);
the front side of box body (1) is the sample position, and the right side is smashing the position, and the rear side is the oscillation position, and the left side is for measuring the position, smash between position and the oscillation position for annotating the liquid level, be the centrifugation position between measuring position and the oscillation position, electric telescopic handle (37) are installed in the interior top of box body (1) being located sample position department, electric telescopic handle (37) can drive bobbin (7) the reciprocating of corresponding position, bobbin (7) pivoted motor (33) can be driven in the bottom of box body (1) being located centrifugation position department.
2. The marine Pythium detection kit as claimed in claim 1, wherein the front side of the kit body (1) is provided with a door body (4) corresponding to the sampling position, and the left side of the kit body (1) is provided with an insertion port (3) corresponding to the measuring position.
3. The marine Pythium detection kit according to claim 2, wherein the two sides of the mounting opening (5) are respectively provided with a strip groove (15), the strip grooves (15) are elastically connected with racks (19) through first springs (22), the strip grooves (15) are rotationally connected with rotating shafts (21), the tail ends of the rotating shafts (21) are fixedly connected with corresponding side support plates (17), and gears (20) meshed with the racks (19) are fixed on the rotating shafts (21).
4. A marine pythium detecting kit according to claim 3, characterized in that the opening and closing mechanism further comprises a push-pull box (8) fixedly connected to the side wall of the bottom of the bobbin (7), and a second spring (34) elastically connected with the opening and closing plate (29) is installed in the push-pull box (8).
5. The marine Pythium detection kit as claimed in claim 4, wherein a chute (32) is formed in the side wall of the supporting plate (17), a sliding block (18) is elastically connected in the chute (32) through a third spring (31), and the side wall of the sliding block (18) is fixedly connected with the swivel (13).
6. The marine Pythium detection kit as claimed in claim 5, wherein the extruding mechanism comprises a cylinder (14) fixedly connected to the top of the bobbin (7), a supporting rod (25) is elastically connected in the cylinder (14) through a fourth spring (24), the lower end of the supporting rod (25) extends into the bobbin (7) and is fixedly connected with a piston (26), and the matched magnetic blocks (23) are respectively fixed at the top of the cylinder (14) and the output end of the motor (33).
7. The marine Pythium detection kit as claimed in claim 6, further comprising a power supply mechanism capable of powering on the first spring (22), the second spring (34), the third spring (31) and the fourth spring (24), wherein the power supply mechanism comprises a conductive component mounted on the rotary table (2) and a power supply component mounted on the inner top of the box body (1), the conductive component comprises a plurality of groups of conductive elements which are arranged on the top of the rotary table (2) and correspond to the bobbins (7) one by one, the groups of conductive elements consist of a first conductive head (9), a second conductive head (10), a third conductive head (11) and a fourth conductive head (12) which are arranged radially from outside to inside, the first conductive head (9) is electrically connected with the first spring (22), the second conductive head (10) is electrically connected with the second spring (34), the third conductive head (11) is electrically connected with the third spring (31), and the fourth conductive head (12) is electrically connected with the fourth spring (24).
8. The marine Pythium detection kit as claimed in claim 7, wherein the power supply assembly comprises a mounting column (38) fixedly connected to the inner top of the box body (1), a first conductive rail (39) matched with a first conductive head (9), a second conductive rail (40) matched with a second conductive head (10), a first conductive seat (41) matched with a third conductive head (11), two second conductive seats (42) matched with a fourth conductive head (12), the first conductive rail (39) circumferentially extends from a crushing position to a centrifugal position, the second conductive rail (40) circumferentially extends from an oversampling position to a filling level, the first conductive seats (41) correspond to an oscillating position and are externally connected with a pulse switch, one second conductive seat (42) is positioned between the centrifugal position and a measuring position, and the other second conductive seat (42) is positioned at the measuring position.
9. The marine Pythium detection kit as claimed in claim 1, wherein a placement opening (36) is formed in the top of the box body (1) corresponding to the crushing position, the placement opening (36) is used for being inserted into crushing equipment, and a liquid injection pipe (35) is arranged in the top of the box body (1) corresponding to the liquid injection position.
10. The marine Pythium detection kit as claimed in claim 1, wherein pressure membrane flaps (28) are mounted at both ends of the liquid outlet hole (30).
CN202310966591.7A 2023-08-03 2023-08-03 Marine Pythium detection kit Active CN116679061B (en)

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