CN116649217B - Proliferation culture method of fig callus - Google Patents
Proliferation culture method of fig callus Download PDFInfo
- Publication number
- CN116649217B CN116649217B CN202310748226.9A CN202310748226A CN116649217B CN 116649217 B CN116649217 B CN 116649217B CN 202310748226 A CN202310748226 A CN 202310748226A CN 116649217 B CN116649217 B CN 116649217B
- Authority
- CN
- China
- Prior art keywords
- callus
- culture medium
- proliferation
- medium
- induction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010020649 Hyperkeratosis Diseases 0.000 title claims abstract description 110
- 230000035755 proliferation Effects 0.000 title claims abstract description 90
- 238000012136 culture method Methods 0.000 title claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 115
- 230000006698 induction Effects 0.000 claims abstract description 35
- 229920001817 Agar Polymers 0.000 claims abstract description 27
- 229930006000 Sucrose Natural products 0.000 claims abstract description 27
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 27
- 239000008272 agar Substances 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 27
- 239000005720 sucrose Substances 0.000 claims abstract description 27
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000005971 1-naphthylacetic acid Substances 0.000 claims abstract description 15
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000012258 culturing Methods 0.000 claims abstract description 13
- HFCYZXMHUIHAQI-UHFFFAOYSA-N Thidiazuron Chemical compound C=1C=CC=CC=1NC(=O)NC1=CN=NS1 HFCYZXMHUIHAQI-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002609 medium Substances 0.000 claims description 43
- 239000005648 plant growth regulator Substances 0.000 claims description 38
- 230000001954 sterilising effect Effects 0.000 claims description 6
- IIDAJRNSZSFFCB-UHFFFAOYSA-N 4-amino-5-methoxy-2-methylbenzenesulfonamide Chemical compound COC1=CC(S(N)(=O)=O)=C(C)C=C1N IIDAJRNSZSFFCB-UHFFFAOYSA-N 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 abstract description 5
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 abstract description 5
- 229940023877 zeatin Drugs 0.000 abstract description 5
- 239000013543 active substance Substances 0.000 abstract description 2
- 102000018997 Growth Hormone Human genes 0.000 abstract 2
- 108010051696 Growth Hormone Proteins 0.000 abstract 2
- 239000000122 growth hormone Substances 0.000 abstract 2
- 230000008635 plant growth Effects 0.000 abstract 2
- 238000012364 cultivation method Methods 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 23
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 16
- 229930003944 flavone Natural products 0.000 description 16
- 150000002212 flavone derivatives Chemical class 0.000 description 16
- 235000011949 flavones Nutrition 0.000 description 16
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 16
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 14
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 238000011081 inoculation Methods 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 239000004480 active ingredient Substances 0.000 description 9
- 239000005556 hormone Substances 0.000 description 8
- 229940088597 hormone Drugs 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 235000019441 ethanol Nutrition 0.000 description 7
- OTCCIMWXFLJLIA-BYPYZUCNSA-N N-acetyl-L-aspartic acid Chemical compound CC(=O)N[C@H](C(O)=O)CC(O)=O OTCCIMWXFLJLIA-BYPYZUCNSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 244000025361 Ficus carica Species 0.000 description 5
- 235000008730 Ficus carica Nutrition 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 4
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 229940074391 gallic acid Drugs 0.000 description 3
- 235000004515 gallic acid Nutrition 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 3
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 3
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 3
- 235000005493 rutin Nutrition 0.000 description 3
- 229960004555 rutoside Drugs 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000004584 weight gain Effects 0.000 description 3
- 235000019786 weight gain Nutrition 0.000 description 3
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 2
- BGEBZHIAGXMEMV-UHFFFAOYSA-N 5-methoxypsoralen Chemical compound O1C(=O)C=CC2=C1C=C1OC=CC1=C2OC BGEBZHIAGXMEMV-UHFFFAOYSA-N 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- HUMNYLRZRPPJDN-UHFFFAOYSA-N benzaldehyde Chemical compound O=CC1=CC=CC=C1 HUMNYLRZRPPJDN-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
- 239000004062 cytokinin Substances 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000003630 growth substance Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 235000017550 sodium carbonate Nutrition 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 description 1
- DBMJZOMNXBSRED-UHFFFAOYSA-N Bergamottin Natural products O1C(=O)C=CC2=C1C=C1OC=CC1=C2OCC=C(C)CCC=C(C)C DBMJZOMNXBSRED-UHFFFAOYSA-N 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960002045 bergapten Drugs 0.000 description 1
- KGZDKFWCIPZMRK-UHFFFAOYSA-N bergapten Natural products COC1C2=C(Cc3ccoc13)C=CC(=O)O2 KGZDKFWCIPZMRK-UHFFFAOYSA-N 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002700 inhibitory effect on cancer Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202310748226.9A CN116649217B (en) | 2023-06-21 | Proliferation culture method of fig callus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202310748226.9A CN116649217B (en) | 2023-06-21 | Proliferation culture method of fig callus |
Publications (2)
Publication Number | Publication Date |
---|---|
CN116649217A CN116649217A (en) | 2023-08-29 |
CN116649217B true CN116649217B (en) | 2024-06-04 |
Family
ID=
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103299901A (en) * | 2013-05-15 | 2013-09-18 | 江苏丘陵地区镇江农业科学研究所 | In-vitro rapid proliferation method of Masui dauphine fig |
CN103518625A (en) * | 2013-11-01 | 2014-01-22 | 重庆文理学院 | Tissue culture medium and in-vitro regeneration method for ficus pandurata blade |
CN113557961A (en) * | 2021-08-19 | 2021-10-29 | 江苏农林职业技术学院 | Method for culturing fig callus |
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103299901A (en) * | 2013-05-15 | 2013-09-18 | 江苏丘陵地区镇江农业科学研究所 | In-vitro rapid proliferation method of Masui dauphine fig |
CN103518625A (en) * | 2013-11-01 | 2014-01-22 | 重庆文理学院 | Tissue culture medium and in-vitro regeneration method for ficus pandurata blade |
CN113557961A (en) * | 2021-08-19 | 2021-10-29 | 江苏农林职业技术学院 | Method for culturing fig callus |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR101786861B1 (en) | Cosmetic composition for heavy metal chelation and particulate matter removal | |
KR101566320B1 (en) | Cosmetic composition comprising an Impatiens balsamina L, Maca, Rosa multiflora Thunberg, Eryobotrya japonica and Pachyrrhizus erosus Complex-fermented extract | |
Jang et al. | Production of biomass and bioactive compounds from shoot cultures of Rosa rugosa using a bioreactor culture system | |
MUSAPHA et al. | Phytochemical constituents in leaves and callus of Ficus deltoidea Jack var. kunstleri (King) Corner | |
KR102134163B1 (en) | A vinegar composition comprising camellia flower extract or the powder of camellia flower extract and a method for preparing the same | |
CN116649217B (en) | Proliferation culture method of fig callus | |
CN110612904B (en) | Tissue culture and rapid propagation medium group of dracocephalum plants and application thereof | |
Kanoma et al. | Qualitative and quantitative phytochemical screening of cola nuts (Cola nitida and Cola acuminata) | |
KR101787337B1 (en) | the fermented samultang extracting method with the function of both anti-oxidation and immunity and the samultang extract using thereof | |
CN116649217A (en) | Proliferation culture method of fig callus | |
KR101496790B1 (en) | Method for increasing growth and bioactive compound content of Eurycoma sp. adventitious root | |
CN115413580A (en) | Culture medium and culture method for promoting psammosilene tunicoides hairy root callus to generate anthocyanin | |
Musbah et al. | Effects of feeding tyrosine or phenylalanine on the accumulation of polyphenols in Coleus Blumei in Vivo and in Vitro. | |
CN104396758A (en) | Dendrobium officinale embryoid culture process | |
KR20170120053A (en) | the fermented samultang extracting method with the function of both anti-oxidation and immunity and the samultang extract using thereof | |
Demirci | Determination of secondary metabolite production efficiency in Echinacea purpurea callus, shoot, and root in vitro cultures with methyl jasmonate applications | |
CN101629161A (en) | Aralia elate seem hormone autotrophic cell line | |
KR20220077695A (en) | A solid tea composition including camellia flower fermentation extract and a method for preparing the same | |
KR101371079B1 (en) | Manufacturing Method of Functional Fermentation Products using CHEJU Scoria and Functional Plants and Culturing Method | |
KR100464704B1 (en) | Manufacturing method of fermented alcoholic drink using medicinal and eatable plants having organic germanium and fermented alcoholic drink obtained therefrom | |
CN109646381A (en) | A kind of moisturizing lotion and preparation method thereof added with plant extracts | |
Qahtan et al. | In vitro regeneration, phytochemical profiling and antioxidant activity in Ruta chalepensis plants established from alginate encapsulated synthetic seeds | |
KR102596185B1 (en) | Method for producing collagen jelly using Aralia continentalis mixed concentrate having anti-inflammatory activity for inflammation from microdust and antioxidant activity | |
Mntovska et al. | Comparative determination of phenolic content and radical scavenging activity of the Bulgarian endemic species Stachys bulgarica Degen & Neic | |
CN114732752B (en) | Composition for skin whitening and use thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20231226 Address after: Room 301, Building 3, No. 112, Jinjiadu Road, Liangzhu Street, Yuhang District, Hangzhou City, Zhejiang Province 310000 Applicant after: Hangzhou Shiguang Xinya Biotechnology Co.,Ltd. Applicant after: Suzhou Shiguang Pharmaceutical Biotechnology Co.,Ltd. Applicant after: Beijing Weiming Shiguang Biotechnology Co.,Ltd. Address before: Room 301, Building 3, No. 112, Jinjiadu Road, Liangzhu Street, Yuhang District, Hangzhou City, Zhejiang Province 310000 Applicant before: Hangzhou Shiguang Xinya Biotechnology Co.,Ltd. |
|
GR01 | Patent grant |