CN116634858A - Application of higher fatty alcohol in improving disease resistance and stress resistance of plants - Google Patents
Application of higher fatty alcohol in improving disease resistance and stress resistance of plants Download PDFInfo
- Publication number
- CN116634858A CN116634858A CN202180003280.XA CN202180003280A CN116634858A CN 116634858 A CN116634858 A CN 116634858A CN 202180003280 A CN202180003280 A CN 202180003280A CN 116634858 A CN116634858 A CN 116634858A
- Authority
- CN
- China
- Prior art keywords
- higher fatty
- fatty alcohol
- improving
- plants
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Abstract
The invention provides an application of higher fatty alcohol in preparing a preparation for improving disease resistance and stress resistance of plants, wherein the preparation containing higher fatty alcohol can improve the disease resistance and stress resistance of plants by improving the transcription level of genes related to a phenylpropane metabolic pathway, improving the transcription level of genes related to an isoflavone biosynthesis pathway and improving the content of isoflavone compounds in the plants.
Description
The invention relates to application of higher fatty alcohol in improving disease resistance and stress resistance of plants.
The plant growth substance regulates the growth and development of plants, thereby affecting the yield, quality and resistance of plants (crops), and playing an important role in promoting or regulating the growth of crops, enhancing the stress resistance of plants, increasing the yield, improving the quality of plants, preserving the quality after harvest and the like; compared with the traditional agricultural technology, the plant growth regulator has the advantages of low cost, quick effect, high benefit, good safety and labor saving, and the use of the plant growth regulator becomes one of the important measures of modern agriculture, thereby being beneficial to the large-scale, intensive and sustainable production of agriculture.
Research shows that lysophosphatidylcholine and lysophosphatidylethanolamine are important signal substances for plants, and can remarkably increase stress resistance of plants and improve postharvest shelf life of agricultural products. In addition, researches show that lysophosphatidylethanolamine can activate the activity of phenylalanine ammonia lyase, a key enzyme of a phenylpropane metabolic pathway, and the phenylpropane metabolic pathway and a branch isoflavone biosynthesis pathway thereof participate in the synthesis of disease-resistant and stress-resistant important substances such as lignin, phytoalexins and the like of plants, and the physiological pathway is upregulated by breeding or induction technology, so that the capability of resisting biological and non-biological stress of crops can be remarkably improved. However, no exogenous substances or using methods which can be externally applied and have no toxicity, low cost and wide application range are available for adjusting the physiological paths of the crops.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide the application of the higher fatty alcohol in improving the disease resistance and stress resistance of plants.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
the invention provides an application of higher fatty alcohol in preparing a preparation for improving the content of lysophospholipid signal substances in plants.
Further, lysophospholipid signaling substances include lysophosphatidylcholine and lysophosphatidylethanolamine.
Further, the higher fatty alcohol can be applied to activating plant immunity and inhibiting plant premature senility by increasing the content of the lysophosphatidylethanolamine of the plant.
In another aspect, the invention provides the use of a higher fatty alcohol for the preparation of a formulation for increasing transcription level of a gene associated with a phenylpropane metabolic pathway in a plant.
In another aspect, the invention provides the use of a higher fatty alcohol for the preparation of a formulation for increasing transcription level of genes associated with the isoflavone biosynthetic pathway in plants.
In another aspect, the invention provides the use of a higher fatty alcohol for the preparation of a formulation for increasing the content of isoflavonoid compounds.
In another aspect, the invention provides an application of higher fatty alcohol in preparing a preparation for improving plant disease resistance, wherein the preparation containing higher fatty alcohol can improve plant disease resistance by improving transcription level of genes related to a phenylpropane metabolic pathway, improving transcription level of genes related to an isoflavone biosynthesis pathway and improving isoflavone compound content of plants.
In another aspect, the invention provides an application of higher fatty alcohol in preparing a preparation for improving plant stress resistance, wherein the preparation containing higher fatty alcohol can be applied to improving plant stress resistance by improving transcription level of genes related to a phenylpropane metabolic pathway, improving transcription level of genes related to an isoflavone biosynthesis pathway and improving isoflavone compound content of plants. Further, the application of the plant in stress resistance comprises the application in sun burn prevention and fruit cracking prevention.
In the present invention, the higher fatty alcohol is one of dodecanol and hexadecanol or a mixture of the two.
Further, the preparation is an aqueous emulsion, which comprises higher fatty alcohol, an emulsifying agent, a thickening agent and water.
The higher fatty alcohol-containing formulation of the present invention is not limited to aqueous emulsion, and is applicable to all dosage forms employing the inventive concept of the present invention, such as wettable powder, emulsion, sprayable solution, concentrated emulsion, aerosol, seed coating.
Compared with the prior art, the invention has the beneficial effects that:
the invention utilizes advanced transcriptome and metabolome sequencing and analysis technology to screen and determine that the emulsifier containing higher fatty alcohol can influence the content of signal substances lysophosphatidylcholine and lysophosphatidylethanolamine, the gene transcription level related to phenylpropane metabolic pathway and isoflavone biosynthesis pathway in plants through external application, and accumulate isoflavone substances, thereby improving the disease resistance and stress resistance of plants.
The invention is described in further detail below with reference to the drawings and the detailed description.
FIG. 1 is a schematic representation of the isoflavone biosynthesis pathway in peanut leaf blades.
Example 1
The embodiment provides an aqueous emulsion containing higher fatty alcohol, which comprises the following components in percentage by weight: 24% of dodecanol, 3% of hexadecanol, 3% of emulsifier, 5% of thickener and the balance of water.
In this embodiment, the emulsifier is polyoxyethylene fatty acid ester and the thickener is methylcellulose.
The embodiment also provides a preparation method of the aqueous emulsion, which comprises the following steps:
heating dodecanol and hexadecanol to 60 ℃ to melt, adding an emulsifying agent into the container, treating by a high-shear homogenizer at 5000 rpm, rotating for 10 minutes, adding 60 ℃ water into the container, treating by the high-shear homogenizer at 10000 rpm, rotating for 10 minutes, cooling to 40 ℃, adding a thickening agent into the container, treating by the high-shear homogenizer, and rotating for 30 minutes at 10000 rpm to obtain the product.
Example 2
The embodiment provides an aqueous emulsion containing higher fatty alcohol, which comprises the following components in percentage by weight: 24% of dodecanol, 3% of emulsifying agent, 5% of thickening agent and the balance of water.
In this embodiment, the emulsifier is polyoxyethylene fatty acid ester and the thickener is methylcellulose.
The embodiment also provides a preparation method of the aqueous emulsion, which comprises the following steps:
heating dodecanol to 60 ℃ to melt, adding an emulsifying agent into the container, treating by a high-shear homogenizer at 5000 rpm, rotating for 10 minutes, adding 60 ℃ water into the container, treating by the high-shear homogenizer at 10000 rpm, rotating for 10 minutes, cooling to 40 ℃, adding a thickening agent into the container, treating by the high-shear homogenizer, and rotating for 30 minutes at 10000 rpm to obtain the product.
Example 3
The embodiment provides an aqueous emulsion containing higher fatty alcohol, which comprises the following components in percentage by weight: 3% of cetyl alcohol, 3% of emulsifying agent, 5% of thickening agent and the balance of water.
In this embodiment, the emulsifier is polyoxyethylene fatty acid ester and the thickener is methylcellulose.
The embodiment also provides a preparation method of the aqueous emulsion, which comprises the following steps:
heating cetyl alcohol to 60 ℃ to melt, adding an emulsifying agent into the container, treating by a high-shear homogenizer at 5000 rpm, rotating for 10 minutes, adding 60 ℃ water into the container, treating by the high-shear homogenizer at 10000 rpm, rotating for 10 minutes, cooling to 40 ℃, adding a thickening agent into the container, treating by the high-shear homogenizer, and rotating for 30 minutes at 10000 rpm to obtain the product.
Example 4
The embodiment provides an aqueous emulsion which comprises the following components in percentage by weight: 3% of emulsifying agent, 5% of thickening agent and the balance of water.
In this embodiment, the emulsifier is polyoxyethylene fatty acid ester and the thickener is methylcellulose.
Placing the emulsifying agent into a container, treating by a high-shear homogenizer at a rotation speed of 5000 rpm for 10 minutes, adding 60-DEG C water into the container, treating by the high-shear homogenizer at a rotation speed of 10000 rpm for 10 minutes, cooling to 40 ℃, adding the thickening agent into the container, treating by the high-shear homogenizer, and rotating for 30 minutes at 10000 rpm to obtain the product.
Example 5: effect of higher fatty alcohol-containing aqueous emulsion on peanut lysophosphatidylcholine and lysophosphatidylethanolamine content
Lysophosphatidylcholine and lysophosphatidylethanolamine are important signal substances in plant cells, and normal physiology derives from the fact that phospholipids are hydrolyzed by phospholipase to form a long carbon chain, and can be induced by various pressures. Lysophosphatidylcholine plays an important role in root nodule formation. The lysophosphatidylethanolamine can activate the activity of phenylalanine ammonia lyase which is a key enzyme related to immunity and non-biological stress resistance, and inhibit premature senility of plants. The aqueous emulsion containing higher fatty alcohol is used for treating peanut samples, performing metabolite measurement and transcriptome sequencing, and analyzing the influence of the aqueous emulsion on the content of peanut lysophosphatidylcholine and lysophosphatidylethanolamine.
(1) Fatty alcohol sample: aqueous emulsions prepared according to the invention as set forth in Table 1
Table 1: sample numbering
(2) Test plants:
table 2: plant for test
Culture conditions: 70/0 mu mol m -2 s -1 (light/dark period light intensity), 14/10 hours (light/dark period time)Room), 27 ℃/24 ℃,70% relative humidity, four weeks of age.
(3) Sample processing and data collection:
selecting healthy plants with similar growth states in groups, and spraying the leaves with the diluent (900 times of water dilution) of the sample until the leaves are completely covered with a liquid film. The control group was sprayed with an equal amount of sterile water for dilution of the stock solution. And carrying out second spraying treatment after 48 hours. Each group was set up with 3 biological replicates.
At 72 hours after the first treatment, 4 gram samples of each leaf were collected. After 3 minutes of liquid nitrogen flash freezing, the metabolome assay was performed with dry ice incubated medium-delivered to the wu-kanmai-teville biotechnology limited laboratory. (all of the following Metabolic and transcriptomic sequencing data are available from Wuhan Mei Viville Biotechnology Co., ltd.)
(4) Effect of treatment on 2 signal species content:
after 72 hours of treatment of peanut and tomato leaves with the mixed formulation of dodecanol and hexadecanol (S), the dodecanol alone alcohol formulation (a), the emulsifier (C) and CK (clear water), the signal substances lysophosphatidylcholine (12:0, peanut; 14:0, tomato) and lysophosphatidylethanolamine content of the physiological metabolites were measured and the results are shown in table 3. The test results show that the content of lysophosphatidylcholine in the mixed preparation is 4070 times higher than that in the peanut leaf treated by the clear water control, and the content of lysophosphatidylcholine in the mixed preparation is 4070 times higher than that in the emulsifier treatment, which indicates that the signal substance is raised by the non-emulsifier. The increase 1850 times in lysophosphatidylcholine content in dodecanol alone compared to the fresh water control and 1850 times in lysophosphatidylcholine content in dodecanol alone compared to the emulsifier alone, indicated that the increase in signal species caused by dodecanol treatment was not caused by the emulsifier either. The content of the signal substances in the emulsifier treatment is consistent with that in the clear water treatment. Also, the mixed formulation had a 960 fold increase in the lysophosphatidylethanolamine content of the other signal substance compared to the clear water control, consistent with the fold increase compared to the emulsifier treatment, indicating that the signal substance content up-regulation was caused by the higher fatty alcohol treatment. The fold rise was consistent for the control and emulsifier treatments compared to the dodecanol alone treatment and was lower than for the dodecanol and hexadecanol treatments, consistent with lysophosphatidylcholine. In each tomato treatment, the content of lysophosphatidylcholine and lysophosphatidylethanolamine is also up-regulated under the action of the mixed preparation (S) of dodecanol and hexadecanol and the dodecanol single alcohol preparation (A).
From the results of the content change of the two signal substances caused by the separate treatment and the mixing treatment of the higher fatty alcohol, it was demonstrated that both the separate treatment and the mixing treatment of the higher fatty alcohol can cause the content up-regulation of the two signal substances.
Table 3: ratio of lysophosphatidylcholine to lysophosphatidylethanolamine content in plant leaves
Labeling of lysophospholipids: 12:0 means 12 carbon without double bonds, 14:0 means 14 carbon without double bonds, 16:3 means 16 carbon with three double bonds, and 14:0 (2 n isomer) means 14 carbon without double bond isomers
Example 6: effect of higher fatty alcohol-containing aqueous emulsion on transcription of various genes involved in the phenylpropane metabolic pathway of representative plants
(1) Fatty alcohol sample: same as in example 5
(2) Test plants:
table 4: plant for test
Culture conditions: 70/0 mu mol m -2 s -1 (light/dark cycle light intensity), 14/10 hours (light/dark cycle time), 24 ℃/22 ℃ (arabidopsis), 27 ℃/24 ℃ (peanut, wheat, rice, tomato, soybean), 70% relative humidity, four weeks old.
(3) Sample processing and data collection As in example 5, transcriptome sequencing was performed by the Ma Mei Teville Biotechnology Co., ltd
(4) Treatment of Effect on the transcription level of Gene involved in the phenylpropane Metabolic pathway
Lysophospholipids are involved in the regulation of phenylalanine ammonia lyase activity, are specific metabolic pathways for the action of signal substances, are one of the most important secondary metabolic pathways for plants, and play an important role in plant growth and development and interaction in plant environment. The pathway includes multiple branches, and generates metabolites such as isoflavone. Isoflavone metabolites are involved in physiological processes such as reducing ultraviolet injury, resisting disease occurrence, tolerating uncomfortable temperatures and high salt drought conditions in plant cells. The content of lysophospholipid signal substances in peanut and tomato leaves can be obviously improved by using several higher fatty alcohol preparations related to the invention, which shows that downstream metabolic pathways can be further influenced. Thus, the transcription level detection of gene related to phenylpropane metabolic pathway is further developed by using S sample, A sample, B sample and clear water to treat peanut, wheat and rice leaves. As shown in Table 5, the results show that the expression levels of some of the important genes were significantly up-regulated, either by dodecanol alone or hexadecanol alone or by a combination of 2. In general, dodecanol and hexadecanol combinations are superior to treatment alone in regulating gene transcript levels. The same research method is applied, and S samples and clear water treatment mode plants of Arabidopsis thaliana, crop tomatoes and soybeans are used for further verifying that the S preparation has similar physiological effects on various plants.
Table 5: ratio of gene transcription level difference related to phenylpropane metabolic pathway in plant leaf
Example 7: effect of higher fatty alcohol-containing aqueous emulsion on peanut isoflavone biosynthesis pathway-related Gene transcription and metabolite content
The phenylpropane metabolic pathway relates to a plurality of physiological activities of plants, and in order to further verify that the higher fatty alcohol preparation can regulate the transcription level and the metabolite content of genes related to the phenylpropane metabolic pathway of the plants, an isoflavone biosynthesis pathway is selected as a target, and peanut leaves are treated by using a 72-hour S sample and clear water, so that the transcription level of genes related to the pathway and the corresponding metabolite histology are measured, and combined analysis is performed. The results are shown in FIG. 1 and tables 6 and 7.
(1) Fatty alcohol sample: same as in example 5
(2) Test plants: peanut (cultivar Shan oil 35),
plant culture conditions: 70/0 mu mol m -2 s -1 (light/dark cycle light intensity), 14/10 hours (light/dark cycle time), 27 ℃/24 ℃,70% relative humidity, four weeks old.
(3) Sample processing and data collection: as in example 5, transcriptome and metabolome assays were performed by the Wohaiteville Biotechnology Co., ltd
(4) Treatment of transcription and metabolite content of genes involved in the isoflavone biosynthetic pathway
The peanut leaf isoflavone biosynthetic pathway is shown in figure 1, wherein the mixed preparation of dodecanol and hexadecanol causes the transcription level of a plurality of genes to be up-regulated after being treated, and gray represents the corresponding gene (rectangular) transcription level or metabolite (circular) content after being treated by the S preparation to be up-regulated obviously compared with the control group. The average up-regulation level of three biological replicates of the relevant genes is shown in Table 6, wherein the 2-hydroxyisoflavone synthase gene transcription level was up-regulated 5.80-fold, isoflavone 4' -O-methyltransferase gene transcription level was up-regulated 4.53-fold, isoflavone/4 ' -methoxyisoflavone 2' -hydroxylase gene transcription level was up-regulated 2.25-fold, and glutamine reductase gene transcription level was up-regulated 4.21-fold. Correspondingly, the levels of several important isoflavonoids are also greatly up-regulated, as shown in Table 7, wherein 3, 9-dihydroxypterocarpan content is up-regulated 1223.67-fold, calycosin is up-regulated 2657.76-fold, and soybean Huang Sushang is up-regulated 1982.35-fold. Research shows that 3, 9-dihydroxypterocarpine is an important plant protection element premise, and can improve the resistance of crops to plant diseases and insect pests; calycosin and daidzein have been shown to have significant stress resistance activity.
Table 6: differential ratio (S/control) of isoflavone biosynthesis pathway related gene transcript levels in peanut plant leaves
Table 7: isoflavone biosynthesis pathway mark metabolite content difference ratio (S/control) in peanut plant leaf
Example 8: effect of higher fatty alcohol-containing aqueous emulsion on physiological phenotype of peanut
Examples 5,6 and 7 show that the aqueous emulsion containing higher fatty alcohol of the present invention can significantly affect the signal substances of peanut, lysophosphatidylcholine and lysophosphatidylethanolamine, and further affect the metabolic pathway of phenylpropane and the biosynthesis of isoflavones, and specific physiological phenotypes of these physiological pathways include disease resistance, yield increase, ultraviolet resistance, etc., and in order to verify that the physiological metabolic pathway caused by higher fatty alcohol of the present invention does have the above-mentioned physiological activities, the verification of the controlled conditions of environmental variables was carried out in a laboratory.
(1) Test varieties: same as in example 7;
(2) Test method:
the peanut seeds are respectively subjected to seed dressing, airing and sowing by using a 150-time diluted S sample, a sample A, a sample B and clear water, and the germination vigor, germination rate, germination index, average germination day and activity index, and physiological and biochemical indexes of plant height, leaf tissue proline content, malondialdehyde (MDA) content, peroxidase (POD) activity and plant dry weight after 8 days of growth under simulated drought conditions are measured by using a 5% polyethylene glycol 6000 rooting treatment after leaf spraying (900-time dilution).
(3) Effects of treatment on peanut physiology
The measurement results of the average number of three biological replicates are shown in tables 9 and 10. The results show that the mixing of dodecanol and hexadecanol can significantly improve the germination vigor of peanuts, which is higher than that of dodecanol or hexadecanol which is treated independently and that of a control, and the dodecanol and hexadecanol which is treated independently are also significantly higher than that of the control, but no difference exists between 2 fatty alcohol treatments which are treated independently. The germination rate, germination index and vitality index all show the same law. The average germination days can be significantly shortened by the mixing treatment and the dodecanol alone treatment, and the germination days of the hexadecanol treatment and the control can be significantly shortened. The result shows that the mixture of dodecanol and hexadecanol has obvious phenotype in the aspects of improving the germination rate and the bud potential of the peanuts and shortening the germination time. Wherein a, b and c represent statistically significant differences in the data, group b is more significant than group c, and group a is more significant than group b.
Table 9: physiological index of peanut seed germination after treatment
The phenylpropane metabolic pathway and the isoflavone metabolite can increase the resistance of plants to stress, and the drought resistance of peanuts can be obviously improved by simulating a drought experiment and determining that the independent or mixed treatment of dodecanol and hexadecanol can obviously improve the plant height of the peanuts, wherein the proline content of leaves of the mixed treatment of the dodecanol and the hexadecanol is obviously higher than that of leaves of the independent treatment and comparison of the dodecanol and the hexadecanol, and the proline content of leaves of the independent treatment of 2 alcohols is also obviously higher than that of the comparison. The content of malondialdehyde is consistent with the content rule of proline. The POD content of the mixed treatment was significantly higher than the other treatments and controls, and the dodecanol and hexadecanol alone treatments were higher than the controls but the differences were not significant. The dry weight data are consistent with POD.
The results of physiological assays further demonstrate that higher fatty alcohols do increase peanut drought resistance by affecting lysophosphatidylcholine and lysophosphatidylethanolamine signal levels.
Table 9: peanut plant physiological index after drought treatment simulation
In conclusion, from the sequencing results of the metabolome and the transcriptome and the test results of the plant physiological phenotype, the higher fatty alcohol preparation for improving the plant signal substances and regulating the metabolic pathway of the plant phenylpropane can obviously improve the lysophosphatidylcholine and the lysophosphatidylethanolamine of the plant signal substances and regulate the transcription level and the metabolite content of genes related to the metabolic pathway of the plant phenylpropane, thereby effectively improving the stress resistance, disease resistance and storage resistance of the plant and improving the quality and increasing the yield of agricultural products.
Example 9: preparation containing higher fatty alcohol for improving stress resistance and yield increase effect of rice
Sampling: sample S is the aqueous emulsion prepared in example 1
Crop: rice fragrant adhesive
Location: shaoguan city, guangdong province, xingxing county, and Ma Zheng
The method comprises the following steps: selecting 50 mu of test group and 50 mu of control group at production base to produce according to table 10
Table 10: the rice test scheme of the preparation of the invention
Comparison of using effects:
the test group rice treated by the aqueous emulsion containing the higher fatty alcohol has no disease hazard in the whole growth process, has obvious advantages in terms of growth vigor and long phase in appearance, has higher plant types, deeper and glossy functional leaf colors, more effective ears, increased grain number per ear and compact ear; the control group was significantly weaker in several respects.
During the experiment, the rice in the control group and the rice in the treatment group are obviously affected in the overcast and rainy weather when the flowering period and the grouting period of the rice meet more than twenty continuous days.
The sampling and yield measuring results are as follows:
table 11: the rice test and yield measurement result of the preparation containing the higher fatty alcohol
| Sequence number | Project | Test group | Control group |
| 1 | Number of empty cases ratio (%) | 6.0 | 13.3 |
| 2 | Thousand grain weight (g) | 20.1 | 15.0 |
| 3 | Wet cereal yield (kg/mu) | 600.3 | 344.3 |
The control group suffers from overcast and rainy weather in the flowering period and the grouting period, so that the pollination grouting of rice is greatly influenced, the number of shrunken rice is increased, the yield is obviously reduced, and the test group has full grains and keeps the normal yield and slightly improves the yield because of the effect generated after the treatment of the aqueous emulsion of the preparation containing the higher fatty alcohol. Compared with the control group, the rice empty shell number of the experimental group is obviously better, the weight of a single rice is far greater than that of the control group, and the yield is 76.6% higher than that of the control group, so that the water emulsion of the preparation containing higher fatty alcohol has obvious adverse resistance and yield increase effects in rice production.
Example 10: stress resistance and yield increase effects of preparation containing higher fatty alcohol in fruit tree production
Stress resistance of plum
Sampling: sample S is the aqueous emulsion prepared in example 1
Crop: plum (Red plum and honey plum)
Location: shaoguan city, guangdong province, xingxing county and dun town
The method comprises the following steps: selecting a 50 mu test group and a 50 mu control group at a production base, wherein the test group is produced as follows: spraying after diluting with sample S with 900 times of water, spraying 1 time each in the beginning flowering period, in the middle of physiological fruit dropping period and in the 3-5 mark of fruit, spraying 15 days at intervals, and using the amount of each sample: 200 mL/mu, the control group was not treated.
Comparison of using effects:
before the plums mature, the plums are basically affected by natural weather laws: after sunning for several days in high temperature weather, heavy rain and heavy rain cause serious fruit cracking and fruit dropping. Compared with a control group, the test group plums treated by the aqueous emulsion of the preparation containing the higher fatty alcohol have no obvious disease hazard in the whole growth process, the crown breadth, the branches and the young shoots are tidy, the young shoots are aged at a higher speed, the cracking and dropping of fruits are obviously reduced (see table 13 and table 14), the fruits are even and smooth, the sugar degree is increased by 3-4, the taste is fresh and natural, and the overall yield is increased by about 30.1%.
Before the plums mature, natural weather causes high temperature strong light for one week, after the weather of medium and heavy rain, ten trees are randomly extracted for fruit dropping and fruit cracking statistics, and the result is as follows:
table 13: the preparation plum fruit drop statistics containing higher fatty alcohol
| Sequence number | Project | Tree numbering | Test group | Control group |
| 1 | Fruit drop number (particle) | 1# | 1 | 13 |
| 2 | Fruit drop number (particle) | 2# | 5 | 9 |
| 3 | Fruit drop number (particle) | 3# | 0 | 14 |
| 4 | Fruit drop number(particle) | 4# | 3 | 8 |
| 5 | Fruit drop number (particle) | 5# | 2 | 25 |
| 6 | Fruit drop number (particle) | 6# | 0 | 19 |
| 7 | Fruit drop number (particle) | 7# | 1 | 16 |
| 8 | Fruit drop number (particle) | 8# | 2 | 16 |
| 9 | Fruit drop number (particle) | 9# | 3 | 21 |
| 10 | Fruit drop number (particle) | 10# | 0 | 11 |
| 11 | Total number of fallen fruits (particles) | 17 | 152 |
Table 14: the preparation plum split fruit statistics containing higher fatty alcohol
| Sequence number | Project | Tree numbering | Test group | Control group |
| 1 | Number of cracked fruits (particle) | 1# | 2 | 7 |
| 2 | Number of cracked fruits (particle) | 2# | 1 | 5 |
| 3 | Number of cracked fruits (particle) | 3# | 0 | 6 |
| 4 | Number of cracked fruits (particle) | 4# | 2 | 15 |
| 5 | Number of cracked fruits (particle) | 5# | 1 | 6 |
| 6 | Number of cracked fruits (particle) | 6# | 1 | 14 |
| 7 | Number of cracked fruits (particle) | 7# | 1 | 10 |
| 8 | Number of cracked fruits (particle) | 8# | 0 | 12 |
| 9 | Number of cracked fruits (particle) | 9# | 1 | 11 |
| 10 | Number of cracked fruits (particle) | 10# | 3 | 9 |
| 11 | Total number of split fruits (particles) | 12 | 95 |
B citrus stress resistance
Sampling: sample S is the higher fatty alcohol-containing aqueous emulsion of the preparation prepared in example 1
Crop: tribute citrus
Location: guangdong Shaoguan city starter Jiang Ou Zhang City
The method comprises the following steps:
selecting a 10 mu test group and a 10 mu control group at a production base, wherein the test group is produced as follows: spraying after diluting with sample S with 900 times of water, spraying 1 time each in the beginning flowering period, in the middle of physiological fruit dropping period and in the 3-5 mark of fruit, spraying 15 days at intervals, and using the amount of each sample: 200 mL/mu, the control group was not treated.
Comparison of using effects:
compared with a control group, the citrus gonggan treated by the aqueous emulsion of the preparation containing the higher fatty alcohol has the advantages of large crown width, uniform and reasonable branch distribution, tender green and smooth leaves, tidy young shoots, quick young shoot aging speed, less fruit cracking, fruit dropping and sunburn (see table 15 and table 16), no diseases such as canker, uniform fruit size, smooth fruit peel and rich fruit fragrance in the whole growth process.
In the third physiological fruit dropping period, ten trees are randomly extracted for fruit dropping and sunscald fruit statistics, and the results are as follows:
table 15: the preparation containing higher fatty alcohol of the invention is used for counting the fruit drop of tribute citrus
| Sequence number | Project | Tree numbering | Test group | Control group |
| 1 | Fruit drop number (particle) | 1# | 0 | 9 |
| 2 | Fruit drop number (particle) | 2# | 0 | 10 |
| 3 | Fruit drop number (particle) | 3# | 4 | 7 |
| 4 | Fruit drop number (particle) | 4# | 1 | 8 |
| 5 | Fruit drop number (particle) | 5# | 0 | 7 |
| 6 | Fruit drop number (particle) | 6# | 0 | 16 |
| 7 | Fruit drop number (particle) | 7# | 1 | 13 |
| 8 | Fruit drop number (particle) | 8# | 0 | 6 |
| 9 | Fruit drop number (particle) | 9# | 3 | 11 |
| 10 | Fruit drop number (particle) | 10# | 0 | 9 |
| 11 | Total number of fallen fruits (particles) | 9 | 93 |
Table 16: the preparation containing higher fatty alcohol of the invention counts the sun-burn fruits of the citrus gonggan
| Sequence number | Project | Tree numbering | Test group | Control group |
| 1 | Sunburn fruit number (particle) | 1# | 0 | 4 |
| 2 | Sunburn fruit number (particle) | 2# | 1 | 5 |
| 3 | Sunburn fruit number (particle) | 3# | 0 | 1 |
| 4 | Sunburn fruit number (particle) | 4# | 0 | 13 |
| 5 | Sunburn fruit number (particle) | 5# | 2 | 7 |
| 6 | Sunburn fruit number (particle) | 6# | 0 | 4 |
| 7 | Sunburn fruit number (particle) | 7# | 1 | 11 |
| 8 | Sunburn fruit number (particle) | 8# | 0 | 11 |
| 9 | Sunburn fruit number (particle) | 9# | 0 | 6 |
| 10 | Sunburn fruit number (particle) | 10# | 1 | 8 |
| 11 | Total number of sunscald fruits (particles) | 5 | 70 |
Example 11: the preparation containing higher fatty alcohol has effect in peanut field production
Sampling: sample S is the aqueous emulsion prepared in example 1
Crop: peanut oil No. 35
Location: guangdong Shaoguan city starter Jiang Ou Zhang City
The method comprises the following steps: selecting a 20 mu test group and a 20 mu control group for production at a production base, wherein the test group is produced as follows: diluting sample S with water 900 times, spraying, and spraying 1 time for each of seedling stage, flowering stage, pod stage and maturation stage, wherein each sample is used in the following amount: 15 mL/mu, a control was applied with a brassinolide product treatment (trade name of Brassica harvest).
The test was conducted by a expert in the peanut soybean industry technical system, guangdong province, the test results were measured, and the conventional components of the products were measured, and the results are shown in tables 17 and 18.
Table 17: peanut test effect by adopting the S preparation of the invention
| Sequence number | Project | Test group | Control group |
| 1 | Index of rotten fruit condition | 0.2121 | 0.4946 |
| 2 | Weight of individual fruits (g) | 81.25 | 65.6 |
| 3 | Fresh fruit mu yield (Kg) | 751.62 | 606.85 |
The table shows that the rotted fruits of the peanuts treated by the preparation containing the higher fatty alcohol are obviously reduced, the prevention effect of rotted fruits reaches 57.12%, and the yield is increased by 23.86%.
Table 18: comparison of conventional ingredients of peanuts with the S preparation of the invention
| Sequence number | Project | Test group | Control group |
| 1 | Moisture (%) | 5.80 | 6.23 |
| 2 | Ash (%) | 2.44 | 2.46 |
| 3 | Crude protein (%) | 25.92 | 23.74 |
| 4 | Crude fat (%) | 47.50 | 43.52 |
The above table shows that the quality of peanuts treated with the higher fatty alcohol-containing formulation of the present invention is much higher than that of the control group.
Examples 5,6 and 7 show that the preparation containing the higher fatty alcohol is diluted by adding water to treat plants, so that plant signal substances can be improved, the metabolic pathway of the plant phenylpropane can be regulated, the higher fatty alcohol composition can obviously improve lysophosphatidylcholine and lysophosphatidylethanolamine of the plant signal substances, and the transcriptional level and the metabolite content of genes related to the metabolic pathway of the plant phenylpropane can be regulated, thereby effectively improving the stress resistance and disease resistance of plants, improving the quality and increasing the yield of agricultural products; from example 8, it is evident that the physiological phenotype test in the laboratory proves that the use of the preparation containing higher fatty alcohol can significantly influence the germination condition of peanut seeds and the stress resistance of plants; example 9 shows that the emulsifier containing higher fatty alcohol of the invention can effectively resist adverse effects of external growth environment, improve quality of agricultural products, increase yield and improve storage resistance of rice; example 10 field use effect shows that the emulsifier containing higher fatty alcohol can effectively resist adverse effects of external growth environment, reduce fruit drop, fruit cracking and sunscald fruit, improve fruit quality and increase yield; example 11 field use shows that the emulsifier containing higher fatty alcohol of the invention can effectively prevent peanut rot, improve peanut quality and increase yield.
By combining the preparation containing the higher fatty alcohol according to the invention from the embodiment 5 to the embodiment 11, the content of plant signal substances lysophosphatidylcholine and lysophosphatidylethanolamine in plant tissues can be improved, the gene transcription level of a plant phenylpropane related pathway and the content of corresponding metabolites can be regulated, thereby increasing the variation of plant related disease resistance and stress resistance pathways and substances, improving the stress resistance, disease resistance and energy resistance of plants in actual agricultural production, improving the quality of agricultural products and improving the yield of agricultural products.
The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, but any insubstantial changes and substitutions made by those skilled in the art on the basis of the present invention are intended to be within the scope of the present invention as claimed.
Claims (10)
- Use of higher fatty alcohols for the preparation of a formulation for increasing the content of lysophospholipid signal substances in plants.
- The use according to claim 1, wherein the lysophospholipid signaling material comprises lysophosphatidylcholine and lysophosphatidylethanolamine.
- Use according to claim 2, wherein the higher fatty alcohol is used to activate plant immunity and to inhibit premature plant senescence by increasing the level of lysophosphatidylethanolamine in the plant.
- Use of higher fatty alcohol in the preparation of a formulation for increasing transcription level of a gene associated with a phenylpropane metabolic pathway in a plant.
- Use of higher fatty alcohol in preparation of preparation for improving transcription level of plant isoflavone biosynthesis pathway related gene is provided.
- The application of higher fatty alcohol in preparing preparation for increasing plant isoflavone compound content is provided.
- The application of the higher fatty alcohol in preparing the preparation for improving the plant disease resistance is characterized in that the preparation containing the higher fatty alcohol can improve the plant disease resistance by improving the transcription level of genes related to the phenylpropane metabolic pathway, improving the transcription level of genes related to the isoflavone biosynthesis pathway and improving the isoflavone compound content of plants.
- The application of the higher fatty alcohol in preparing the preparation for improving the stress resistance of plants is characterized in that the preparation containing the higher fatty alcohol realizes the application in improving the stress resistance of plants by improving the transcription level of genes related to the metabolic pathway of phenylpropane, improving the transcription level of genes related to the biosynthetic pathway of isoflavone and improving the content of isoflavone compounds in plants.
- The use according to claim 8, wherein the use of plants for stress tolerance comprises the use of sun burn protection, fruit crack protection.
- The use according to any one of claims 1 to 9, wherein the higher fatty alcohol is one of dodecanol, hexadecanol or a mixture of both.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2021/128773 WO2023077374A1 (en) | 2021-11-04 | 2021-11-04 | Application of higher fatty alcohol in improvement of disease resistance and stress resistance of plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116634858A true CN116634858A (en) | 2023-08-22 |
Family
ID=86240344
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202180003280.XA Pending CN116634858A (en) | 2021-11-04 | 2021-11-04 | Application of higher fatty alcohol in improving disease resistance and stress resistance of plants |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN116634858A (en) |
| WO (1) | WO2023077374A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116406229A (en) * | 2021-11-04 | 2023-07-07 | 珠海市润农科技有限公司 | Application of higher fatty alcohol in promoting synthesis of lignin of rice and wheat and increasing yield |
| CN116406229B (en) * | 2021-11-04 | 2024-04-26 | 珠海市润农科技有限公司 | Application of higher fatty alcohol in promoting synthesis of lignin of rice and wheat and increasing yield |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108378028A (en) * | 2018-03-23 | 2018-08-10 | 渭南高新区促花王科技有限公司 | A kind of novel high polymer panniculus auxiliary agent and preparation method thereof |
| CN108402091A (en) * | 2018-05-31 | 2018-08-17 | 佛山市高明区爪和新材料科技有限公司 | A kind of water-saving antitranspirant of plant and preparation method thereof |
| CN109924057A (en) * | 2019-03-08 | 2019-06-25 | 珠海市润农科技有限公司 | Utilize the method for photosynthetic film prevention and control Citrus Huanglongbing pathogen diffusion high in fat |
| CN109769538A (en) * | 2019-03-08 | 2019-05-21 | 珠海市润农科技有限公司 | Utilize the method for photosynthetic film prevention and control balsam pear drosophila high in fat |
| CN109769539A (en) * | 2019-03-08 | 2019-05-21 | 珠海市润农科技有限公司 | Utilize the method for photosynthetic film prevention control tomato in plastic greenhouse trialeurodes vaporariorum high in fat |
| CN109845770A (en) * | 2019-03-08 | 2019-06-07 | 珠海市润农科技有限公司 | The repellent sustained release film formulation and preparation method and purposes of prevention and treatment drosophila |
| CN109874579A (en) * | 2019-03-08 | 2019-06-14 | 珠海市润农科技有限公司 | Utilize the method for photosynthetic film prevention and control fresh edible maize rust high in fat |
| CN110140564A (en) * | 2019-06-04 | 2019-08-20 | 珠海市润农科技有限公司 | Utilize the method for photosynthetic film prevention and control tobacco virus high in fat |
-
2021
- 2021-11-04 CN CN202180003280.XA patent/CN116634858A/en active Pending
- 2021-11-04 WO PCT/CN2021/128773 patent/WO2023077374A1/en active Application Filing
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116406229A (en) * | 2021-11-04 | 2023-07-07 | 珠海市润农科技有限公司 | Application of higher fatty alcohol in promoting synthesis of lignin of rice and wheat and increasing yield |
| CN116406229B (en) * | 2021-11-04 | 2024-04-26 | 珠海市润农科技有限公司 | Application of higher fatty alcohol in promoting synthesis of lignin of rice and wheat and increasing yield |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023077374A1 (en) | 2023-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Colla et al. | Foliar applications of protein hydrolysate, plant and seaweed extracts increase yield but differentially modulate fruit quality of greenhouse tomato | |
| Dordas | Foliar boron application improves seed set, seed yield, and seed quality of alfalfa | |
| Sadeghi et al. | Effect of 24-epibrassinolide on salinity-induced changes in loquat (Eriobotrya japonica Lindl) | |
| Luo et al. | Strigolactones affect the translocation of nitrogen in rice | |
| Jayaprakash | Pollen germination in vitro | |
| Xu et al. | Super rice with high sink activities has superior adaptability to low filling stage temperature | |
| Chen et al. | Seed dormancy release and germination requirements of Cinnamomum migao, an endangered and rare woody plant in southwest China | |
| Sukifto et al. | Enhancing germination and early seedling growth of Malaysian indica rice (Oryza sativa L.) using hormonal priming with gibberellic acid (GA 3). | |
| CN116744783A (en) | Application of higher fatty alcohol in improving nitrogen fixation capacity and drought resistance capacity of leguminous plants | |
| Bandyopadhyay et al. | Sorghum grain mold: through the 1990s into the new millennium | |
| Bakhoum et al. | Induction of tolerance in groundnut plants against drought stress and Cercospora leaf spot disease with exogenous application of arginine and sodium nitroprusside under field conditions | |
| CN116634858A (en) | Application of higher fatty alcohol in improving disease resistance and stress resistance of plants | |
| Tonguç | Seed chemical composition of endemic plant Fraxinus ornus subsp. cilicica. | |
| PAUL | Tiller productivity and survival as determinants of grain yield for selected rice germplasm | |
| Skogen et al. | Effect of triacontanol on production and quality of flowers of Chrysanthemum morifolium Ramat | |
| Eisa et al. | Mitigation the harmful effects of diluted sea water salinity on physiological and anatomical traits by foliar spray with trehalose and sodium nitroprusside of cowpea plants | |
| Andrade et al. | Morphological, physiological, and biochemical indicators of quality in tobacco fruits and seeds | |
| Nuanlaong et al. | Lysigenous aerenchyma formation: responsiveness to waterlogging in oil palm roots | |
| CN116406229B (en) | Application of higher fatty alcohol in promoting synthesis of lignin of rice and wheat and increasing yield | |
| Yue et al. | Auxins regulations of branched spike development and expression of TFL, a LEAFY-Like Gene in Branched Spike Wheat (Triticum aestivum) | |
| VIDAL-LEZAMA et al. | Warm Dry Storage and Application of Gibberellins in The Lipid Profile of Chincuya Seeds (Annona purpurea Moc. & Sessé ex Dunal) | |
| Suraweera et al. | Effect of short periods of heat stress during early flowering period on flower development and pyrethrin accumulation in pyrethrum | |
| Anuragi et al. | Breeding for Developing Higher Productive Tree-Based Forage Under Stress Environments | |
| WO2023077377A1 (en) | Application of higher fatty alcohol in fatty acid metabolic pathway of cruciferous plant | |
| Hanumanthappa et al. | Mechanism and duration of seed dormancy in paddy genotypes. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Peng Xiaoming Inventor after: Sun Yunhao Inventor after: Cai Dianxian Inventor after: Yu Guohui Inventor after: Qin Di Inventor before: Peng Xiaoming Inventor before: Sun Yunhao Inventor before: Cai Dianxian Inventor before: Yu Guohui Inventor before: Qin Di |
|
| CB03 | Change of inventor or designer information |