CN1166267A - Application method of direct seading rice - Google Patents
Application method of direct seading rice Download PDFInfo
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- CN1166267A CN1166267A CN97109815A CN97109815A CN1166267A CN 1166267 A CN1166267 A CN 1166267A CN 97109815 A CN97109815 A CN 97109815A CN 97109815 A CN97109815 A CN 97109815A CN 1166267 A CN1166267 A CN 1166267A
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Abstract
The direct rice seeding method includes transfering antiphytocide gene into the rice breed to make it obtain anti-phytocide character; direct seeding of transgenotic rice; and spreading phytocide to kill weeds and remained rice stubble of preceeding rice in rice field. By using the said method, the directly seeding rice is safe during spreading phytocide, so that the present invention can reduce labour strength and cost of rice production.
Description
The present invention relates to a kind of application method of direct seading rice.
The many in the world paddy rice plantations of rice direct-seeding technology country is by widespread usage, and China is faced with rapid economic development, and it is imperative to widely popularize the rice direct-seeding technology.Yet China's south rice planting area mostly is two season plantations, and the plant of eliminating the farmland running to weeds of direct sowing rice field and other rice varieties that preceding stubble rice field stays is to realize China's two difficult problems that season is live or the single cropping late rice is live in double cropping rice area.Whole nation accumulative total direct seading rice cultivated area is 1,000 ten thousand mu during " eight or five ", expectation the enforcement period of the ninth five-year plan will be developed into 3,000 ten thousand mu, if can solve the farmland running to weeds problem that runs into when direct seading rice is produced, national directly seeding rice area will be developed by leaps and bounds, and obtains good economic and social benefit.
Anti-herbicide gene (bar) separates from moist streptomycete (Strep Tomyceshygroscopicus) and obtains, its phosphino-thricin (PPT) transacetylase (PAT) of encoding.This gene outcome (PAT) can make the acetylization of PPT terminal amino group.Bialaphos in the weed killer herbicide and PPT are main active ingredient, for a kind of L-glutamic acid analog, are the potent inhibitors of glutamy synzyme.It is generally acknowledged that the PPT that generates in the plant corpus can suppress the glutamy synzyme and lead the accumulation of system ammonia, plant is poisoned to death because of the ammonia accumulation.Because the bar gene PAT that can encode, make the free amine group acetylization on the PPT in the Basta weed killer herbicide and remove the toxicity of PPT.This gene has been used for the Study on Transformation of various plants, is one of screening marker gene that produces the high-efficient transgenic plant.
Development of biology is that the successful Application of direct seading rice has been brought hope.
The object of the present invention is to provide a kind of direct seading rice application method that can overcome above-mentioned weak point of the prior art.
The object of the present invention is achieved like this:
A kind of application method of direct seading rice may further comprise the steps: anti-herbicide gene is changed in the direct seading rice kind, make this kind obtain the antiweed characteristic; Genetically modified direct seading rice kind is sowed down, and herbicide spraying is to kill the plant that weeds in paddy field and preceding stubble paddy rice stay.
The anti-herbicide gene that is adopted is the bar gene in the present invention, but it will be apparent to those skilled in the art that other anti-herbicide gene of separating clone can be applicable among the present invention equally.
In the present invention, the rice varieties that is used for the transgenosis transformation is selected from the capital and draws 119, hundred No. 1, elegant water 11 and spring river 03, and but, design of the present invention can be applicable to other direct seading rice kind equally, even can be used for the plant of other kind.
In the present invention, because employing is the bar gene, used weed killer herbicide is basta.It will be understood by those skilled in the art that then used weed killer herbicide also can be done corresponding adjustment if what adopt is other anti-herbicide gene.
The method that anti-herbicide gene is changed in the direct seading rice kind is a method known in those skilled in the art, comprises the agriculture bacillus mediated conversion method, electric shocking method, cotransformation method and the particle gun blast technique that are widely adopted at present.The particle gun blast technique is adopted in special recommendation of the present invention.
Advantage of the present invention is:
Can make the direct seading rice kind obtain anti-weeding gene after using the inventive method, therefore, after spraying the careless agent in danger, both can eliminate weeds in paddy field, the plant that the stubble paddy rice stays before eliminating again, and it is fool proof for the direct seading rice kind, really reach the light-duty agricultural cultivation of time and labour saving, can reduce labor intensity, can reduce agricultural cost again.
Fig. 1 is paddy rice transfer-gen plant and the non-transgenic plant photo to the Herbicid resistant result of the test.Wherein, A represents that the non-transgenic plant is killed by weed killer herbicide, and B represents that the transfer-gen plant growth is normal.
With way of example the inventive method is done more detailed description below.
In June, 1996, by experimental scheme, as the direct seading rice kind, system is sowed by strain for seed (band anti-herbicide gene bar) with transgenosis T3, each strain system sowing 40 strain in the land for growing field crops, Hangzhou.Compare (not containing anti-herbicide gene bar) every 20 row kind of a non-transgenic rice varieties Zhenshan 97a,, spray the 0.25%Basta weed killer herbicide in full heading time, week back observation, the result is as shown in Figure 1.All growths are normal in the picture, and it is colony for transgenosis T3 for strain that blade keeps green person, because of leading the people anti-herbicide gene bar is arranged, and the toxicity that this gene product ox can be separated PPT in the weed killer herbicide can not cause and be poisoned to death, and weed killer herbicide Basta is shown as strong resistance; Otherwise non-transgenic common rice kind plant lacks anti-herbicide gene, and the PPT composition makes ammonia accumulation causing poisoning in these paddy rice bodies among the weed killer herbicide Basta, and blade is withered and yellow to cause death, and is all killed by weed killer herbicide Basta in a week.Obviously, after weed killer herbicide Basta sprays, when killing weeds in paddy field, can also kill batch other rice varieties plant that stays (lacking the bar gene) before the rice field.And it is fool proof to the rice plant that contains anti-herbicide gene bar.
According to The above results, after anti-herbicide gene imports the direct seading rice kind, weeds in paddy field and removing Always just being readily solved of other rice varieties plant that stubble stays before the rice field both alleviated the peasant and worked by force Degree guarantees again direct sowing rice field and seed purity, can not cause all the time to mix, and demonstrates fully the time and labour saving Superiority.
Claims (5)
1, a kind of application method of direct seading rice may further comprise the steps:
Anti-herbicide gene is changed in the direct seading rice kind, make this kind obtain the antiweed characteristic;
Genetically modified direct seading rice kind is sowed down, and herbicide spraying is to kill the plant that rice is stayed by weeds and preceding stubble paddy rice.
2, the method for claim 1 is characterized in that: described anti-herbicide gene is the bar gene.
3, the method for claim 1 is characterized in that: described direct seading rice kind be selected from the capital draw 119, in hundred No. 1, elegant water 11 and spring river 03.
4, the method for claim 1 is characterized in that: described weed killer herbicide is Basta.
5, the method for claim 1 is characterized in that: described transgenic method comprises agriculture bacillus mediated conversion method, electric shocking method, cotransformation method or particle gun blast technique.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN971098158A CN1064811C (en) | 1997-04-28 | 1997-04-28 | Application method of direct seading rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN971098158A CN1064811C (en) | 1997-04-28 | 1997-04-28 | Application method of direct seading rice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1166267A true CN1166267A (en) | 1997-12-03 |
| CN1064811C CN1064811C (en) | 2001-04-25 |
Family
ID=5171217
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN971098158A Expired - Fee Related CN1064811C (en) | 1997-04-28 | 1997-04-28 | Application method of direct seading rice |
Country Status (1)
| Country | Link |
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| CN (1) | CN1064811C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926552A (en) * | 2017-11-20 | 2018-04-20 | 湖南杂交水稻研究中心 | A kind of removable rice field or seed farm shattering paddy seedling and the implantation methods of weeds |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL83348A (en) * | 1986-08-26 | 1995-12-08 | Du Pont | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
| EP0262489B1 (en) * | 1986-09-26 | 1990-06-13 | Veit Gmbh & Co | Seam pressing device |
| JPH01132320A (en) * | 1987-11-17 | 1989-05-24 | Mitsui Toatsu Chem Inc | Efficient production of seed of hybrid of rice plant |
| DK0461355T3 (en) * | 1990-05-09 | 1997-08-25 | American Cyanamid Co | Method of preventing crop damage in the presence of synergistic pesticide combinations |
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1997
- 1997-04-28 CN CN971098158A patent/CN1064811C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926552A (en) * | 2017-11-20 | 2018-04-20 | 湖南杂交水稻研究中心 | A kind of removable rice field or seed farm shattering paddy seedling and the implantation methods of weeds |
| CN107926552B (en) * | 2017-11-20 | 2019-08-09 | 湖南杂交水稻研究中心 | A kind of removable rice field or seed farm shattering paddy seedling and the implantation methods of weeds |
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| Publication number | Publication date |
|---|---|
| CN1064811C (en) | 2001-04-25 |
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