CN116622760B - Application of LuAccD gene in regulating synthesis of plant fatty acid and salt tolerance and drought resistance - Google Patents
Application of LuAccD gene in regulating synthesis of plant fatty acid and salt tolerance and drought resistance Download PDFInfo
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Abstract
The invention provides an application of LuAccD genes in regulating the synthesis of plant fatty acid, wherein after LuAccD genes are overexpressed in plants, the synthesis amount of the plant fatty acid can be improved. The invention also provides application of LuAccD genes in regulating salt tolerance and drought resistance of plants, and after LuAccD genes are overexpressed in plants, salt tolerance and drought resistance of the plants can be improved. The invention provides application of LuAccD gene for regulating synthesis of plant fatty acid and salt tolerance and drought resistance by exploring functions of LuAccD gene in lipid metabolism of plant seeds and response to abiotic stress, and fills up the blank of LuAccD gene in genetic engineering application.
Description
Technical Field
The invention belongs to the technical field of plant molecular biology, relates to LuAccD genes, and in particular relates to application of LuAccD genes to regulation of plant fatty acid synthesis and salt tolerance and drought resistance.
Background
Acetyl-coa carboxylase is a rate-limiting enzyme for de novo synthesis of fatty acids, and is widely found in a variety of organisms, both heterogeneous and homogeneous, and both types have biotin carboxyl carrier protein subunits, biotin carboxylase subunits, α -CT subunits and β -CT subunits of carboxytransferase. During fatty acid accumulation in seeds, the activity of acetyl-coa carboxylase is proportional to the rate of fatty acid synthesis. The AccD gene encoding the beta-CT subunit is overexpressed in tobacco plastids, promoting leaf and seed fatty acid synthesis. The AccD gene for encoding the escherichia coli heterogeneous acetyl-CoA carboxylase beta-CT subunit is specifically expressed in rape seeds, so that the oil content of the rape seeds can be obviously improved. Four subunits of ACCase in upland cotton are mutually coordinated to jointly regulate and control the oil content of seeds, and the oil content of upland cotton seeds can be obviously improved by over-expressing GhBCCP, ghBC1, ghalpha-CT and Ghbeta-CT genes.
The acetyl-CoA carboxylase beta-CT subunit coding gene of flax (namely AccD gene of flax) is highly expressed in developed seeds, but the function of the gene in the metabolic and stress response processes of vegetable oil is not clear, so that the genetic engineering application of the flax AccD gene is limited.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention aims to provide the LuAccD gene for regulating the synthesis of plant fatty acid and the application of salt tolerance and drought resistance, and solve the technical problem that the function of the LuAccD gene in the prior art is not clear, so that the application of genetic engineering is limited.
In order to solve the technical problems, the invention adopts the following technical scheme:
the LuAccD gene is used for regulating the synthesis of plant fatty acid, and after the LuAccD gene is overexpressed in plants, the synthesis amount of the plant fatty acid can be improved.
The LuAccD gene is used for regulating the salt tolerance and drought resistance of plants, and after the LuAccD gene is overexpressed in the plants, the salt tolerance and drought resistance of the plants can be improved.
The nucleotide sequence of the LuAccD gene is shown as sequence ID Number 1 in a nucleotide or amino acid sequence table; the amino acid sequence coded by LuAccD gene is shown as sequence ID Number in nucleotide or amino acid sequence table.
The invention also has the following technical characteristics:
specifically, the LuAccD gene is over-expressed in the plant, so that the transcription level of the genes related to the accumulation of the vegetable oil can be improved; the grease accumulation related genes comprise: atBCCP1 gene, atBCCP gene, atMCAT gene, atKAS gene, atKAS gene, atSSI gene, atFAD gene, atFAD gene and AtPDAT gene.
Specifically, the LuAccD gene is over-expressed in the plant, so that the salt tolerance and drought resistance of the plant can be improved; the nucleotide sequence of LuAccD gene is shown as sequence ID Number 1 in nucleotide or amino acid sequence table.
Specifically, the LuAccD gene can reduce the transcription level of the gene related to stress response in the plant after being over-expressed in the plant; the stress response related genes include: atNCED3 gene, atABI gene, atAAO gene, atEM1 gene and AtEM6 gene.
Specifically, plants were able to tolerate 150mM sodium chloride and 300mM mannitol after the LuAccD gene was overexpressed in the plants.
Specifically, the method for over-expressing LuAccD genes in plants comprises the following steps:
Connecting LuAccD genes to an overexpression vector pGreen-35S-6HA to obtain a 35S: luAccD-6HA overexpression vector, and transferring the 35S: luAccD-6HA overexpression vector into plants; the nucleotide sequence of the overexpression vector pGreen-35S-6HA is shown as sequence ID Number in a nucleotide or amino acid sequence table.
Specifically, the plant is a wild type plant.
The invention also protects a method of overexpressing LuAccD genes in plants as described above.
Compared with the prior art, the invention has the following technical effects:
The invention provides application of LuAccD gene for regulating synthesis of plant fatty acid and salt tolerance and drought resistance by exploring functions of LuAccD gene in grease metabolism and response of abiotic stress of plant seeds, and fills up the blank of LuAccD gene in genetic engineering application.
And (II) the method has important significance for analyzing the regulation mechanism of enzyme activity molecules in the vegetable seed oil metabolism process, improving the content and components of the flax fatty acid and cultivating a novel flax-resistant variety.
Drawings
FIG. 1 is a schematic representation of the insertion position of LuAccD gene in the overexpression vector pGreen-35S-6 HA. In fig. 1: RB represents the right border, LB represents the left border, NOS-pro represents the promoter; NOS-ter indicates a terminator, basta indicates a glufosinate resistance screening gene, and 35S-pro indicates a 35S strong promoter.
FIG. 2 is a nucleic acid electrophoretogram of PCR identification of transgenic line seeds. In fig. 2: cas represents 35S: luAccD-6HA overexpression vector.
FIG. 3 is a histogram of qRT-PCR identification LuAccD of the expression level of genes in transgenic line seeds.
FIG. 4 is a macroscopic morphology of wild type Arabidopsis seeds and transgenic line seeds.
FIG. 5 is a histogram of thousand kernel weight of wild type Arabidopsis seeds and transgenic lines.
FIG. 6 is a histogram of length and width of wild type Arabidopsis seed and transgenic strain seed.
FIG. 7 is a histogram of total fatty acid content in wild type Arabidopsis seeds and transgenic line seeds.
FIG. 8 is a histogram of the content of each fatty acid component in wild type Arabidopsis seeds and transgenic line seeds.
FIG. 9 is a histogram of qRT-PCR detection of expression levels of oil accumulation-related genes in wild type Arabidopsis seeds and transgenic line seeds.
FIG. 10 is a graph showing germination states of wild type Arabidopsis seeds and transgenic strain seeds under salt and mannitol stress. In fig. 10: 1/2 MS represents 1/2 MS medium, 150mM NaCl 150mM sodium chloride, 300mM Mannitol 300mM Mannitol.
FIG. 11 is a histogram of germination rates of wild type Arabidopsis seeds and transgenic lines under salt and mannitol stress. In fig. 11: 1/2 MS represents 1/2 MS medium, 150mM NaCl 150mM sodium chloride, 300mM Mannitol 300mM Mannitol.
FIG. 12 is a histogram of expression levels of stress response related genes in wild type Arabidopsis seeds and transgenic line seeds under salt stress.
The following examples illustrate the invention in further detail.
Detailed Description
All reagents, kits, enzymes and media used in the present invention are those known in the art, for example:
the plant RNA extraction kit is purchased from Hunan Ai Kerui bioengineering Co., ltd, and has the product number of No. AG21019.
Reverse transcription kit, available from Beijing full gold Biotechnology Co., ltd., product No. AE311.
High fidelity DNA polymerase, available from baori doctor materials technology (beijing) limited under the designation No. r045q.
DNA purification recovery kit, available from Tiangen Biochemical technology (Beijing) Co., ltd., product No. DP214.
Restriction enzymes EcoR I and Xma I, manufactured by NEW ENGLAND Biolabs Inc.
One-step cloning kit, commercially available from Nanjinouzan Biotechnology Co., ltd, under the trade name ClonExpress II One Step Cloning Kit.
Plasmid extraction kit, produced by Omega Bio-Tek company, U.S.A.
The herbicide is produced by Bayer company and has the trade name of Basta herbicide, and its effective component is glufosinate-ammonium, and its content is 20% [ v/v ].
The 1L LB liquid medium comprises the following components: 10g of tryptone, 5g of yeast extract and 10g of NaCl; the LB solid medium is prepared by adding 15g of agar based on the above formula.
1/2 MS medium, available from Beijing Soy Bao technology Co., ltd., product No. M8527.
In the invention, the following components are added:
Flax, flax "ridged No. 10" is known in the art and is cultivated by the institute of crop research, academy of agricultural sciences, gansu province.
The following specific embodiments of the present application are provided, and it should be noted that the present application is not limited to the following specific embodiments, and all equivalent changes made on the basis of the technical scheme of the present application fall within the protection scope of the present application.
Example 1:
The present example shows the application of LuAccD gene in regulating fatty acid synthesis in plant, and the application can raise the fatty acid synthesis amount of plant after LuAccD gene is over expressed in plant.
As a specific scheme of the embodiment, the method for over-expressing LuAccD genes in plants specifically comprises the following steps:
Step one, luAccD gene cloning:
Step 1.1, obtaining a LuAccD gene sequence and designing a specific primer:
A AtAccD protein sequence is obtained through a Tair website of an Arabidopsis database, a BLAST P program is operated in an NCBI database according to the Arabidopsis AtAccD protein sequence to obtain a homologous sequence in flax, the homologous sequence is named as LuAccD gene, the nucleotide sequence of LuAccD gene is shown as sequence ID Number 1 in a nucleotide or amino acid sequence table, and the amino acid sequence encoded by LuAccD gene is shown as sequence ID Number in the nucleotide or amino acid sequence table.
The insertion position of LuAccD gene on the vector was designed and determined as shown in FIG. 1; specific primers (35S: luAccD-6HA-EcoR I-F and 35S: luAccD-6HA-Xma I-R) were then designed using the Primer BLAST program in NCBI, while verification primers (35S-F) on the vector were designed, and the Primer sequences are shown in Table 1.
TABLE 1 primer sequences
| Primer name | Primer sequence (5 '. Fwdarw.3') |
| 35S:LuAccD-6HA-EcoR I-F | GATAAGCTTGATATCGAATTCATGACTAGTTCAGATAGAAT |
| 35S:LuAccD-6HA-Xma I-R | GTATGGGTAACTAGAACTAGTATGAGTCAAAGCGTGGAGA |
| 35S-F | GACCCTTCCTCTATATAAGGAAGTTC |
Step 1.2, rna extraction and cDNA synthesis:
extracting total RNA of the seeds in the flax germination period by using a plant RNA extraction kit, and synthesizing cDNA of the seeds in the flax germination period by using a reverse transcription kit by taking the total RNA of the seeds in the flax germination period as a template.
Step 1.3, luaccd gene cloning:
Adopting the specific primer designed in the step 1.1, taking cDNA of the flax seeds synthesized in the step 1.2 in the germination period as a template, using high-fidelity DNA polymerase, amplifying target genes by a PCR technology, wherein a reaction system is as follows: 10 XPCR Buffer 2.5 μ L, mg 2+ 1 μ L, dNTP 1 μ L, KOD-Plus 0.5 μL,1 μ L, cDNA template for each of the upstream and downstream primers 1 μL, sterile water up to 25 μL; the PCR reaction conditions are shown in Table 2.
TABLE 2 high fidelity PCR reaction conditions
And (3) after the PCR reaction is finished, agarose gel electrophoresis is carried out, whether the size of the target gene is correct or not is judged according to DNA MARKER, the strip with the correct size is cut, and the target gene fragment is purified and recovered by using a universal DNA purification and recovery kit.
Step two, constructing a plant expression vector:
Step 2.1, linking the target gene fragment with a linear vector:
The overexpression vector pGreen-35S-6HA and the gene fragment of interest obtained in step 1.3 were digested overnight at 37℃with the restriction enzymes EcoRI and Xma I. The digested product was purified and recovered using a DNA purification recovery kit. And (3) mixing the purified target fragment with the enzyme-cleaved vector according to a molar ratio of 2:1 by adopting a one-step cloning kit, and carrying out recombination reaction for 30 minutes at 37 ℃ to complete the connection of the target gene fragment and the linear vector. The nucleotide sequence of the overexpression vector pGreen-35S-6HA is shown as sequence ID Number in the nucleotide or amino acid sequence table.
Step 2.2, conversion of ligation product:
The ligation product obtained in the step 2.1 was added to E.coli competent cells DH 5. Alpha. And gently blotted, mixed and placed on ice for 20 minutes, water-bath at 42℃for 90 seconds, rapidly placed on ice for 2 minutes, 700. Mu.L of LB liquid medium was added, and after 30 minutes of shaking culture at 37℃in a shaker, the bacterial solution was spread on LB solid medium plates containing 50. Mu.g.mL -1 kanamycin, and the incubator at 37℃was inverted overnight.
Step 2.3, monoclonal screening and identification:
And (3) singly picking 10 monoclonal colonies from the overnight-cultured flat plate in the step (2.2), respectively placing the colonies in 7 mu L of ddH 2 O, uniformly mixing, absorbing 1 mu L of bacterial liquid as a template, and carrying out colony PCR verification by using the primers 35S-F and 35S designed in the step (1.1) to LuAccD-6HA-Xma I-R. After the PCR reaction was completed, the product was subjected to agarose gel electrophoresis, and 2 correctly banded monoclonal colonies were selected and added to LB liquid medium containing 50. Mu.g.mL -1 kanamycin, and shake cultured at 28℃for 20 hours at 220 rpm.
Sequencing the cultured bacterial liquid, wherein the sequencing result is the same as the target gene sequence, which indicates that LuAccD genes are successfully cloned into an overexpression vector pGreen-35S-6 HA.
Step 2.4, obtaining 35S: luAccD-6HA overexpression vector:
And (3) screening and identifying the correct plasmid bacterial liquid in the step (2.3) for amplification culture, and then carrying out plasmid extraction according to the specification of a plasmid extraction kit to obtain the 35S/LuAccD-6 HA over-expression vector.
Step three, genetic transformation, screening and identification of arabidopsis thaliana:
Step 3.1, transformation and colony identification:
Transferring the 35S:LuAccD-6HA over-expression vector obtained in the step 2.4 into an agrobacterium competent cell GV3101, picking a monoclonal colony, and carrying out colony PCR verification by adopting the primers 35S-F and 35S:LuAccD-6HA-Xma I-R designed in the step 1.1.
Step 3.2, culturing and collecting thalli of positive colonies:
The positive colony verified by PCR in the step 3.1 is added into 200mL of LB liquid medium containing 50 mug.mL -1 kanamycin and 50 mug.mL -1 rifamycin for expansion culture until the bacterial liquid OD 600 is 1.8-2.0, and the bacterial liquid is collected by centrifugation at 4000rpm for 10 minutes at room temperature.
Step 3.3, adopting an agrobacterium inflorescence dip-dyeing method to transfect wild arabidopsis:
Resuspending the bacterial cells collected in the step 3.2 to an OD 600 value of 0.8-1.0 by using an Arabidopsis thaliana conversion solution, wherein the Arabidopsis thaliana conversion solution contains 5 percent of sucrose with the concentration of 0.02 percent of Silwet L-77 surfactant; the inflorescence of the whole plant is soaked in the arabidopsis thaliana transformation solution for 30-40 seconds, the plant is taken out, placed in a tray at one side for dark culture for 2 days, then normal light culture is carried out, and seeds of T 0 generations are harvested after the plant is mature.
Step 3.4, screening and identifying transgenic plants:
Uniformly broadcasting the T 0 generation seeds harvested in the step 3.3 on nutrient soil, culturing in the dark at 4 ℃ for 2-3 days, and transferring to an illumination culture room for continuous culture. After 2 true leaves grow, continuously spraying herbicide with the working concentration of 0.06% [ v/v ] to screen out resistant plants which can still continue to grow after the seedlings are continuously sprayed for about 1 week; transferring the screened resistant plants to new nutrient soil for continuous growth, extracting plant tender leaf DNA (deoxyribonucleic acid) in a rosette stage, performing PCR (polymerase chain reaction) amplification by using specific primers 35S-F and 35S designed in the step 1.1, namely LuAccD-6HA-Xma I-R, and continuously culturing the identified T 1 generation positive transgenic plants until mature and harvesting T 2 generation seeds as shown in a result of a figure 2; and sowing the T 2 generation seeds on an MS culture medium containing 10 mug.mL -1 glufosinate, and continuing screening and culturing to finally obtain the T 3 generation homozygous seeds.
In this example, the expression level of LuAccD gene in T 3 generation homozygous seeds was identified using the specific primers 35S-F and 35S designed in step 1.1, and as shown in FIG. 3, luAccD gene in T 3 generation homozygous seeds was expressed at the transcription level as shown in FIG. 3.
Effect verification of example 1:
To examine the effect of LuAccD genes on plant oil accumulation-related genes, qRT-PCR primers were designed as shown in Table 3:
TABLE 3 qRT-PCR primers for grease accumulation related genes
| Primer name | Primer sequence (5 '. Fwdarw.3') |
| RT-qPCR-AtBCCP1-F | TCACTCAAACCTCCTCGCAC |
| RT-qPCR-AtBCCP1-R | TTGTGCTTTCACCACAGGGT |
| RT-qPCR-AtBCCP2-F | AACCCAATGGGATCTCCTTTCCCT |
| RT-qPCR-AtBCCP2-R | ATAAATTCAGAGAGCTCGGCGGGT |
| RT-qPCR-AtMCAT-F | TCATGGAACCAGCAGTCTCG |
| RT-qPCR-AtMCAT-R | CTGGAGATGTCACCTGGCG |
| RT-qPCR-AtKAS1-F | TCGATTTCAACTGCTTGTGC |
| RT-qPCR-AtKAS1-R | CCTCCCAACCCAATAGGAAT |
| RT-qPCR-AtKAS2-F | TGCCTATCACATGACCGAGC |
| RT-qPCR-AtKAS2-R | CCAAAACAGTGAGCAAGGGC |
| RT-qPCR-AtSSI2-F | CGCTGTGCATAAGCATTCTC |
| RT-qPCR-AtSSI2-R | TTGGGGCCGGAGCTGAGAGC |
| RT-qPCR-AtFAD2-F | ATGGGTGCAGGTGGAAGAAT |
| RT-qPCR-AtFAD2-R | CCAGGAGAAGTAAGGGACGA |
| RT-qPCR-AtFAD3-F | CCACAGTACTCGGATGCTCAGA |
| RT-qPCR-AtFAD3-R | GCAATAAGCTTTCTCTCGCTTGGA |
| RT-qPCR-AtPDAT2-F | AGATGATGAGACGAGCCGAAGC |
| RT-qPCR-AtPDAT2-R | TCTCTGGTGCCTCCGGTAATTTG |
| RT-qPCR-AtFAB2-F | CGCTGTGCATAAGCATTCTC |
| RT-qPCR-AtFAB2-R | TTGGGGCCGGAGCTGAGAGC |
| RT-qPCR-AtKCS17-F | GTCGAGCCCTCGGTTAACAA |
| RT-qPCR-AtKCS17-R | GCCTTCTGGTACATGGAAACC |
(A) The phenotypes of wild type Arabidopsis seeds (designated Col-0) and transgenic strain seeds (designated Col-0 35S: luAccD-6 HA) were analyzed and the results are shown in FIGS. 4,5 and 6. From FIGS. 4,5 and6, heterologous expression of LuAccD genes did not affect seed length, width, thousand kernel weight and seed coat color.
(B) The total fatty acid content and the individual fatty acid component content of the wild type Arabidopsis seeds and transgenic line seeds were analyzed, and the results are shown in FIGS. 7 and 8. As can be seen from fig. 7 and 8, the total fatty acid content and the individual fatty acid component content of the transgenic line seeds were significantly higher than those of the wild-type arabidopsis seeds.
(C) Wild type Arabidopsis seeds and transgenic strain seeds are selected, and the transcription level of the genes related to oil accumulation is detected by adopting qRT-PCR technology, and the result is shown in figure 9. As can be seen from FIG. 9, the gene expression levels of AtBCCP1, atBCCP2, atMCAT, atKAS1, atKAS2, atSSI2, atFAD2, atFAD3, atPDAT2 in Col-0 35S: luAccD-6HA#4 were significantly higher than those of wild type Arabidopsis seeds.
(D) From the analysis of the above (A), (B) and (C), luAccD genes can up-regulate the expression of the genes related to oil accumulation so as to promote the biosynthesis of fatty acid in Arabidopsis seeds; the fatty acid synthesis amount can be improved by over-expressing LuAccD genes in arabidopsis, and other characters of arabidopsis seed development are not affected.
Example 2:
The embodiment provides an application of LuAccD genes for regulating the salt tolerance and drought resistance of plants, and after LuAccD genes are overexpressed in the plants, the salt tolerance and drought resistance of the plants can be improved. In this example, the method of overexpressing LuAccD gene in plants was exactly the same as in example 1.
Effect verification of example 2:
to examine the effect of LuAccD genes on genes associated with stress response, qRT-PCR primers were designed as shown in Table 4:
TABLE 4 qRT-PCR primers for genes related to stress response
| Primer name | Primer sequence (5 '. Fwdarw.3') |
| RT-qPCR-AtABI3-F | TCCATTAGACAGCAGTCAAGGTTT |
| RT-qPCR-AtABI3-R | GGTGTCAAAGAACTCGTTGCTATC |
| RT-qPCR-AtAAO3-F | GGAGTCAGCGAGGTGGAAGT |
| RT-qPCR-AtAAO3-R | TGCTCCTTCGGTCTGTCCTAA |
| RT-qPCR-AtNCED3-F | AGGTCGTGTGAGTTCTTATG |
| RT-qPCR-AtNCED3-R | CACTGGTAAATCTCGCTCTC |
| RT-qPCR-AtEM1-F | TAGGGCACGAGGGTTATCAG |
| RT-qPCR-AtEM1-R | CGCTCTCCACCAGATTTTTC |
| RT-qPCR-AtEM6-F | GCAAACTCGAAAGGAGCAGT |
| RT-qPCR-AtEM6-R | TCTCGACTCCTTCCTCCTCA |
(E) Seed germination experiments under 150mM sodium chloride and 300mM mannitol stress conditions were performed on wild type Arabidopsis seeds and transgenic line seeds, and the results are shown in FIGS. 10 and 11. As can be seen from fig. 10 and 11, under normal conditions, there was no significant difference in germination rate between wild-type arabidopsis seeds and transgenic line seeds; however, under 150mM sodium chloride and 300mM mannitol stress conditions, the germination rate of transgenic line seeds was significantly higher than that of wild type Arabidopsis seeds.
(F) The expression levels of the stress response genes AtNCED, atABI3, atAAO3, atEM and AtEM6 in germination of wild type Arabidopsis seeds and transgenic line seeds under sodium chloride stress were detected using qRT-PCR technology, and the results are shown in FIG. 12. As can be seen from fig. 12, the expression levels of the stress response related genes AtNCED3, atABI3, atAAO3, atEM1 and AtEM6 in the transgenic line seeds were significantly reduced compared to the wild type arabidopsis seeds.
(G) From the analysis of the (E) and the (F), luAccD genes improve the salt tolerance of the seeds in the germination period of the arabidopsis through regulating and controlling the expression of the genes related to stress response; the LuAccD genes are overexpressed in the arabidopsis thaliana, so that the tolerance of the seeds in the germination period of the arabidopsis thaliana to salt and mannitol can be improved.
The effect verification of the comprehensive examples 1 and 2 shows that LuAccD genes have great potential functions in improving the fatty acid content of seeds, and transgenic lines with high oil content, salt tolerance and drought resistance can be obtained by using transgenic technology to overexpress the genes in oil crops.
Claims (10)
- The application of the LuAccD gene in regulating the synthesis of plant fatty acid can improve the synthesis amount of the plant fatty acid after the LuAccD gene is overexpressed in the plant; the nucleotide sequence of LuAccD gene is shown in SEQ ID NO. 1; the plant is Arabidopsis thaliana.
- 2. Use of LuAccD gene for regulating plant fatty acid synthesis according to claim 1, wherein after overexpression of LuAccD gene in plants, the transcription level of genes related to plant oil accumulation can be increased; the grease accumulation related genes comprise: atBCCP1 gene, atBCCP gene, atMCAT gene, atKAS gene, atKAS gene, atSSI gene, atFAD gene, atFAD gene and AtPDAT gene.
- 3. The use of LuAccD gene according to claim 1 for modulating plant fatty acid synthesis, wherein said method of overexpressing LuAccD gene in a plant comprises:Connecting LuAccD genes to an overexpression vector pGreen-35S-6HA to obtain a 35S: luAccD-6HA overexpression vector, and transferring the 35S: luAccD-6HA overexpression vector into plants; the nucleotide sequence of the overexpression vector pGreen-35S-6HA is shown as sequence ID Number in a nucleotide or amino acid sequence table.
- 4. Use of the LuAccD gene according to any one of claims 1 to 3 for modulating plant fatty acid synthesis, wherein the plant is a wild type plant.
- The application of the LuAccD gene in regulating the salt tolerance and drought resistance of plants can improve the salt tolerance and drought resistance of plants after LuAccD genes are overexpressed in the plants; the nucleotide sequence of LuAccD gene is shown in SEQ ID NO. 1; the plant is Arabidopsis thaliana.
- 6. Use of LuAccD gene for regulating salt tolerance and drought resistance in a plant according to claim 5, wherein after overexpression of LuAccD gene in the plant, the level of transcription of the gene associated with stress response in the plant is reduced; the stress response related genes include: atNCED3 gene, atABI gene, atAAO gene, atEM1 gene and AtEM6 gene.
- 7. Use of LuAccD gene for regulating salt tolerance and drought resistance in plants according to claim 5, wherein after overexpression of LuAccD gene in plants, plants are tolerant to 150 mM sodium chloride and 300 mM mannitol.
- 8. The use of LuAccD gene for regulating salt tolerance and drought tolerance in a plant according to claim 5, wherein said method of overexpressing LuAccD gene in a plant comprises:Connecting LuAccD genes to an overexpression vector pGreen-35S-6HA to obtain a 35S: luAccD-6HA overexpression vector, and transferring the 35S: luAccD-6HA overexpression vector into plants; the nucleotide sequence of the overexpression vector pGreen-35S-6HA is shown as sequence ID Number in a nucleotide or amino acid sequence table.
- 9. Use of the LuAccD gene according to any one of claims 5 to 8 for modulating salt tolerance and drought tolerance in a plant, wherein said plant is a wild type plant.
- 10. A method of overexpressing LuAccD genes in plants, comprising: connecting LuAccD genes to an overexpression vector pGreen-35S-6HA to obtain a 35S: luAccD-6HA overexpression vector, and transferring the 35S: luAccD-6HA overexpression vector into plants; the nucleotide sequence of the overexpression vector pGreen-35S-6HA is shown as sequence ID Number in a nucleotide or amino acid sequence table; the nucleotide sequence of LuAccD gene is shown in SEQ ID NO. 1.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1906302A (en) * | 2003-12-05 | 2007-01-31 | 香港大学 | Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS |
| EP2039770A2 (en) * | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
| CN101550421A (en) * | 2009-03-13 | 2009-10-07 | 山东省花生研究所 | Earthnut acetyl coenzyme A carboxylase carboxyltransferase beta subunit gene and encoding protein and cloning method thereof |
| EP2204094A1 (en) * | 2008-12-29 | 2010-07-07 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants Introduction |
| CN103813709A (en) * | 2011-06-28 | 2014-05-21 | 布鲁克黑文科学联合会 | Modified plants with increased oil content |
| CN104270946A (en) * | 2011-12-19 | 2015-01-07 | 拜耳农作物科学股份公司 | Use of anthranilic acid diamide derivatives for pest control in transgenic crops |
| EP3372081A2 (en) * | 2010-12-01 | 2018-09-12 | Bayer CropScience Aktiengesellschaft | Use of fluopyram for controlling nematodes in crops |
| CN113481212A (en) * | 2021-08-03 | 2021-10-08 | 江苏省中国科学院植物研究所 | Chloroplast genome of aronia melanocarpa and application thereof |
| TW202341864A (en) * | 2022-04-14 | 2023-11-01 | 美商富曼西公司 | Novel sulfonate benzamide compounds for controlling invertebrate pests |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007009957A1 (en) * | 2006-12-27 | 2008-07-03 | Bayer Cropscience Ag | Process for improving the use of the productive potential of a transgenic plant e.g. maize, soya bean, cotton, tobacco, rice, comprises treating the plant with 3-aryl-pyrrolidin-2,4-dione compound |
| EP2113172A1 (en) * | 2008-04-28 | 2009-11-04 | Bayer CropScience AG | Method for improved utilisation of the production potential of transgene plants |
-
2023
- 2023-04-06 CN CN202310358219.8A patent/CN116622760B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1906302A (en) * | 2003-12-05 | 2007-01-31 | 香港大学 | Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS |
| EP2204094A1 (en) * | 2008-12-29 | 2010-07-07 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants Introduction |
| EP2039770A2 (en) * | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
| CN101550421A (en) * | 2009-03-13 | 2009-10-07 | 山东省花生研究所 | Earthnut acetyl coenzyme A carboxylase carboxyltransferase beta subunit gene and encoding protein and cloning method thereof |
| EP3372081A2 (en) * | 2010-12-01 | 2018-09-12 | Bayer CropScience Aktiengesellschaft | Use of fluopyram for controlling nematodes in crops |
| CN103813709A (en) * | 2011-06-28 | 2014-05-21 | 布鲁克黑文科学联合会 | Modified plants with increased oil content |
| CN104270946A (en) * | 2011-12-19 | 2015-01-07 | 拜耳农作物科学股份公司 | Use of anthranilic acid diamide derivatives for pest control in transgenic crops |
| CN113481212A (en) * | 2021-08-03 | 2021-10-08 | 江苏省中国科学院植物研究所 | Chloroplast genome of aronia melanocarpa and application thereof |
| TW202341864A (en) * | 2022-04-14 | 2023-11-01 | 美商富曼西公司 | Novel sulfonate benzamide compounds for controlling invertebrate pests |
Non-Patent Citations (6)
| Title |
|---|
| Linum usitatissimum AccD Enhances Seed Fatty Acid Accumulation and Tolerance to Environmental Stresses during Seed Germination in Arabidopsis thaliana;Rui Du 等;《PLANTS》;20230829;全文 * |
| Linum usitatissimum chloroplast, complete genome;NCBI;《Genbank Database》;20230403;accession No.NC_036356.1 * |
| The Linum usitatissimum L. plastome reveals atypical structural evolution, new editing sites, and the phylogenetic position of Linaceae within Malpighiales;Amanda de Santana Lopes 等;《Plant Cell Rep》;20171030;全文 * |
| 利用基因工程改良植物脂肪酸和提高植物含油量的研究进展;夏晗;王兴军;李孟军;肖寒;;生物工程学报;20100625(06);全文 * |
| 多不饱和脂肪酸对基因表达的影响及其机理;许友卿;逄劭楠;丁兆坤;;饲料工业;20110125(02);全文 * |
| 大豆脂肪及脂肪酸组分含量的遗传学研究进展;彭琛琛;;科技经济市场;20090815(08);全文 * |
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