CN116605509B - Mesenchymal stem cell activity detection kit - Google Patents
Mesenchymal stem cell activity detection kit Download PDFInfo
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- CN116605509B CN116605509B CN202310894710.2A CN202310894710A CN116605509B CN 116605509 B CN116605509 B CN 116605509B CN 202310894710 A CN202310894710 A CN 202310894710A CN 116605509 B CN116605509 B CN 116605509B
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- sensing element
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 25
- 238000001514 detection method Methods 0.000 title claims abstract description 19
- 230000000694 effects Effects 0.000 title claims abstract description 17
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 48
- 238000001816 cooling Methods 0.000 claims abstract description 28
- 238000007789 sealing Methods 0.000 claims abstract description 11
- 238000005192 partition Methods 0.000 claims description 24
- 238000004146 energy storage Methods 0.000 claims description 10
- 230000000452 restraining effect Effects 0.000 claims description 10
- 230000002093 peripheral effect Effects 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 230000004888 barrier function Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/20—External fittings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/24—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Food Science & Technology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of detection, and particularly discloses a mesenchymal stem cell activity detection kit, which comprises a kit body, wherein a kit opening is formed in the side edge of the kit body, the kit opening is communicated with the inside of the kit body, and a sealing unit is arranged at the kit opening; the top of the kit body is provided with a cooling unit for cooling the kit body; the upper wall surface and the lower wall surface in the kit body are respectively provided with a constraint cylinder, the two constraint cylinders are arranged opposite to each other, and a placing unit for placing a reagent bottle is connected between the two constraint cylinders; the invention solves the problems that the existing mesenchymal stem cell activity detection kit cannot enable a reagent bottle to be in a constant low-temperature state when in use, the safe transfer or temporary storage of the mesenchymal stem cells in the reagent bottle cannot be ensured, and meanwhile, the number of the reagent bottles which can be placed in the kit is small and the reagent bottles cannot be placed in a classified manner.
Description
Technical Field
The invention belongs to the technical field of mesenchymal stem cell activity detection, and particularly relates to a mesenchymal stem cell activity detection kit.
Background
In different stages of development and under specific conditions, the mesenchymal stem cells have high proliferation and self-renewal capacity, and a kit is needed to be used for detecting the mesenchymal stem cells, and the kit is a box for containing chemical reagents for detecting chemical components, drug residues, virus types and the like.
The prior art CN218401656U discloses a mesenchymal stem cell activity detection kit, and when the kit is used, a low-temperature ice bag layer is placed between the side wall of a detection kit body and a heat preservation sponge layer, the low-temperature ice bag layer is utilized to ensure that a reagent bottle can be in a low-temperature state, but the low-temperature ice bag layer is utilized to keep the low-temperature mode, so that the reagent bottle cannot be in a constant low-temperature state, the safe transfer or temporary storage of the mesenchymal stem cells in the reagent bottle cannot be ensured, and meanwhile, the quantity of the reagent bottles which can be placed in the kit is less, and the reagent bottles cannot be placed in a classified manner.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a mesenchymal stem cell activity detection kit, which solves the problems that when the traditional mesenchymal stem cell activity detection kit is used, a low-temperature ice bag layer is utilized to keep a low temperature, a reagent bottle cannot be in a constant low-temperature state, the safe transfer or temporary storage of the mesenchymal stem cells in the reagent bottle cannot be ensured, and meanwhile, the quantity of the reagent bottles which can be placed in the kit is small and the reagent bottles cannot be placed in a classified mode.
The invention aims at realizing the following technical scheme:
the mesenchymal stem cell activity detection kit comprises a kit body, wherein a box opening is formed in the side edge of the kit body, the box opening is communicated with the inside of the kit body, and a sealing unit is arranged at the box opening;
the top of the kit body is provided with a cooling unit for cooling the kit body;
the upper wall surface and the lower wall surface in the kit body are respectively provided with a constraint cylinder, the two constraint cylinders are arranged opposite to each other, and a placing unit for placing a reagent bottle is connected between the two constraint cylinders;
the sealing unit comprises a leakage-proof baffle and an outer baffle; the inner wall of the box opening is fixedly provided with a square frame, a strip-shaped channel is arranged on the wall surface of the square frame facing the outside, the strip-shaped channel is communicated in a tail-to-tail mode, and a leakage-proof ring I is clamped in the strip-shaped channel;
the side of the wall surface of the square frame facing the outside and the joint of the wall surface and the inner wall of the box opening are provided with a second leakage preventing ring, a magnet is arranged in the second leakage preventing ring, four side walls of the leakage preventing partition plate are provided with a third leakage preventing ring which is attracted with the second leakage preventing ring, and the magnet is arranged in the third leakage preventing ring; the outer partition plate is a hollow square plate and is arranged at the outer side of the leakage-proof partition plate, two groups of constraint pieces are fixedly connected to two sides of the outer partition plate respectively, a knob is screwed on the constraint pieces, and the knob is obliquely arranged; wedge-shaped tables which are mutually matched with the knob to restrict the outer partition plate to move are fixedly arranged on the two side walls of the box opening;
the placing unit comprises a hollow shaft and a plurality of square strips fixedly connected to the outer wall surface of the hollow shaft in a staggered manner, a plurality of through holes are formed in the square strips at equal intervals, a pair of square guide holes are formed in the inner peripheral surface of each through hole, and a bottle locking unit for locking the reagent bottle is arranged on each through hole;
the bottle body locking unit comprises an inner shell matched with the reagent bottle body, a pair of symmetrical concave shells are arranged on two sides of the inner shell, and the concave shells are communicated with the inner shell; the tops of the inner shell and the concave shell are fixedly connected with protruding sheets, and the protruding sheets are clamped above the through holes; the side wall of the concave shell is provided with a square opening; the concave shell is provided with a wedge block penetrating through the square opening, the wedge block is obliquely arranged, the other two sides of the inner shell are provided with a pair of symmetrical concave grooves, and the concave grooves are communicated with the inner shell.
Further, the leak-proof partition board adopts a heat insulation board structure, and a pair of square through holes are formed in the outer wall of the leak-proof partition board.
Further, the cooling unit comprises a chip, a first channel, a second channel, a cooling system, a temperature sensing element, a pressure sensing element, an energy storage box and a touch screen; the first channel passes through the top of the kit body and is communicated with the restraining cylinder above the kit body, and the second channel passes through the top of the kit body and is communicated with the kit body; the cooling system is communicated with the first channel through the first switch, and the second channel is provided with the second switch; the temperature sensing element, the pressure sensing element and the energy storage box are all fixedly connected to the top of the kit body, one ends of the temperature sensing element and the pressure sensing element penetrate into the kit body, the energy storage box, the temperature sensing element, the switch I, the switch II, the pressure sensing element and the touch screen are all electrically connected with the chip, and the touch screen is arranged on the outer wall surface of the kit body.
Further, a plurality of conducting ports are arranged on the wall surface of the hollow shaft at equal intervals.
Further, the two longitudinal ends of the hollow shaft are both communicated with a connecting cylinder, the diameter of the connecting cylinder is smaller than that of the hollow shaft, a telescopic piece is fixedly connected to the connecting cylinder, and the connecting cylinder is communicated with a restraining cylinder.
Further, an upper shell for protecting the cooling unit is fixedly connected to the top of the kit body; a plurality of ventilation openings are formed in the peripheral wall of the upper shell.
The beneficial effects of the invention are as follows:
1. the invention is formed by matching the reagent box body and the sealing unit, the cooling unit cools the interior of the reagent box body, the interior of the reagent box body is always ensured to be in a constant low-temperature state, the placing unit and the bottle locking unit lock the reagent bottles, and the reagent bottles are distributed in the interior of the reagent box body in a staggered manner, so that the reagent bottles can be taken and placed, and a large number of reagent bottles can be placed and classified.
2. The sealing unit is formed by the double arrangement of the leakage-proof partition plate and the outer partition plate, so that the constant temperature inside the kit body can be ensured, and the impact resistance of the kit body can be enhanced.
3. The invention ensures the inside of the kit body to be in a constant low-temperature state during the transfer by the cooling unit so as to facilitate the safe transfer of the mesenchymal stem cells and promote the safety during the transfer.
4. The hollow shafts on the placing unit are provided with the conducting ports at equal intervals so as to facilitate the balanced circulation of air in the reagent box body, ensure the constant temperature in the reagent box body, and the telescopic parts arranged at the two ends of the hollow shafts can buffer the vibration received during the transfer, and square strips are arranged in a staggered manner so as to facilitate the taking of reagent bottles and the sorting placement of different samples.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention may be realized and attained by the structure particularly pointed out in the written description and drawings.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention. In the drawings:
FIG. 1 is a schematic perspective view of an embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of an upper housing according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a front view structure of an embodiment of the present invention;
FIG. 4 is a schematic view of the cross-sectional structure at M-M of FIG. 3 according to the present invention;
FIG. 5 is a schematic view of a structure of a sealing unit according to an embodiment of the present invention;
FIG. 6 is an enlarged schematic view of the structure of FIG. 5 at C in accordance with the present invention;
FIG. 7 is a schematic view of a mounting unit according to an embodiment of the present invention;
FIG. 8 is an enlarged schematic view of the structure of FIG. 7 at D in accordance with the present invention;
FIG. 9 is a schematic view of a leak-proof separator according to an embodiment of the present invention;
FIG. 10 is a schematic view of an outer separator according to an embodiment of the present invention;
FIG. 11 is a schematic top view of an outer separator according to an embodiment of the present invention;
fig. 12 is a schematic view of a bottle locking unit according to an embodiment of the present invention.
Reference numerals: 1. a kit body; 2. a sealing unit; 3. a cooling unit; 4. a placement unit; 5. a leak-proof separator; 6. an outer partition; 7. a leakage preventing ring I; 8. a second leakage preventing ring; 9. a leakage preventing ring III; 10. a bottle body locking unit; 101. an inner housing; 102. a concave shell; 103. a tab; 104. a square mouth; 105. wedge blocks; 106. a concave groove; 11. a box opening; 12. a restraining cylinder; 13. a square frame; 14. a strip-shaped ditch; 15. a wedge-shaped table; 16. an upper housing; 31. a chip; 32. a first channel; 33. a second channel; 34. a cooling system; 35. a temperature sensing element; 36. a first switch; 37. a second switch; 41. a hollow shaft; 42. square strips; 43. a through hole; 44. a guide port; 45. a conduction port; 46. a connecting cylinder; 47. a telescoping member; 51. square through openings; 61. a restraining sheet; 62. a knob; 17. a pressure sensing element; 18. a touch screen; 19. an energy storage box.
Detailed Description
In order to make the objects, technical solutions and advantages of the technical solutions of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of specific embodiments of the present invention. Like reference numerals in the drawings denote like parts. It should be noted that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be made by a person skilled in the art without creative efforts, based on the described embodiments of the present invention fall within the protection scope of the present invention.
Referring to fig. 1-12, an embodiment of the invention provides a mesenchymal stem cell activity detection kit, which comprises a kit body 1, wherein a kit opening 11 is arranged on the side edge of the kit body 1, and the kit opening 11 is communicated with the inside of the kit body 1; a sealing unit 2 is arranged at the box opening 11; the top of the kit body 1 is provided with a cooling unit 3 for cooling the kit body 1; the upper and lower wall surfaces in the kit body 1 are provided with restraining barrels 12; the pair of restraining barrels 12 are arranged opposite to each other, and a placing unit 4 for placing reagent bottles is connected between the two restraining barrels 12.
Referring to fig. 4, 5 and 6, the sealing unit 2 includes a leakage-proof barrier 5 and an outer barrier 6; the inner wall of the box opening 11 is fixedly provided with a square frame 13, the wall surface of the square frame 13 facing the outside is provided with a strip-shaped channel 14, the head and the tail of the strip-shaped channel 14 are communicated with each other, and a leakage-proof ring I7 is clamped in the strip-shaped channel 13; the square frame 13 is equipped with leak protection circle two 8 towards outside wall face avris and the junction with box mouth 11 inner wall, leak protection circle two 8's inside is equipped with the magnet, leak protection circle two 8 and leak protection baffle 5 closely suction each other.
Referring to fig. 6 and 9, the leak-proof partition board 5 adopts a heat insulation board structure; the four side walls of the leakage-proof baffle plate 5 are provided with leakage-proof rings III 9 which are attracted with the leakage-proof rings II 8, and a magnet is also arranged in the leakage-proof rings III 9; a pair of square through holes 51 are formed in the outer wall of the leakage-proof partition plate 5, and the square through holes 51 are used for assisting in assembling the leakage-proof partition plate 5.
Referring to fig. 5, 6, 10 and 11, the outer partition plate 6 is a hollow square plate and is arranged at the outer side of the leakage-proof partition plate 5, two groups of constraint pieces 61 are fixedly connected to two sides of the outer partition plate 6 respectively, a knob 62 is screwed on the constraint pieces 61, and the knob 62 is obliquely arranged; one end of the knob 62 is inclined outwards towards the outer wall of the outer partition plate 6, and wedge-shaped tables 15 which are mutually matched with the knob 62 to restrict the movement of the outer partition plate 6 are fixedly arranged on the two side walls of the box opening 11.
Referring to fig. 2, the cooling unit 3 includes a chip 31, a first channel 32, a second channel 33, a cooling system 34, a temperature sensing element 35, a pressure sensing element 17, an energy storage tank 19 and a touch screen 18;
the first channel 32 passes through the top of the kit body 1 and is communicated with the restraint barrel 12 above the kit body 1, and the second channel 33 passes through the top of the kit body 1 and is communicated with the kit body 1; the cooling system 34 is communicated with the first channel 32 through a first switch 36, and the second channel 33 is provided with a second switch 37; the temperature sensing element 35, the pressure sensing element 17 and the energy storage box 19 are fixedly connected to the top of the kit body 1, one ends of the temperature sensing element 35 and the pressure sensing element 17 penetrate into the kit body 1, and the touch screen 18 is arranged on the outer wall surface of the kit body 1; the energy storage box 19, the temperature sensing element 35, the first switch 36, the second switch 37, the pressure sensing element 17 and the touch screen 18 are all electrically connected with the chip 31, the temperature sensing element 35 is of a type DS18B20, the pressure sensing element 17 is of a type BMP180, the chip 31 is of a type STM32F103C, the touch screen 18 is of a type TPC1031KT, and the first switch 36 and the second switch 37 are of a type ZBSF-16P; the touch screen 18 inputs a low temperature value and a pressure value, the first switch 36 is opened by the chip 31, the cooling system 34 works, the reagent box body 1 is cooled and reduced to the low temperature value, the temperature sensing element 35 and the pressure sensing element 17 transmit the low temperature signal and the pressure signal in the reagent box body 1 to the chip 31, when the pressure in the reagent box body 1 is greater than the input pressure value, the second switch 37 is opened, and then the air is exhausted to the outside, so that the aim of pressure relief is achieved, and after the pressure is constant, the second switch 37 is closed, and the low temperature storage of mesenchymal stem cells is ensured; referring to fig. 2, the cooling system 34 includes an external housing and a compressor, piping, evaporator and condenser mounted therein.
Referring to fig. 4, 7 and 8, the placement unit 4 comprises a hollow shaft 41 and a plurality of square strips 42 fixedly connected to the outer wall surface of the hollow shaft 41 in a staggered manner, a plurality of through holes 43 are formed in the square strips 42 at equal intervals, a pair of square guide holes 44 are formed in the inner peripheral surface of the through holes 43, and a bottle locking unit 10 for locking the reagent bottle is arranged on the through holes 43; a plurality of through holes 45 are formed in the wall surface of the hollow shaft 41 at equal intervals, two longitudinal ends of the hollow shaft 41 are both communicated with a connecting cylinder 46, the diameter of the connecting cylinder 46 is smaller than that of the hollow shaft 41, a telescopic piece 47 is fixedly connected to the connecting cylinder 46, and the connecting cylinder 47 is communicated with the restraining cylinder 12.
Referring to fig. 1, 2 and 5, an upper housing 16 for protecting the cooling unit 3 is fixedly connected to the top of the kit body 1; the peripheral wall of the upper casing 16 is provided with a plurality of ventilation openings, and the upper casing 16 can protect the cooling unit 3 during transfer so as to facilitate safe transfer of the kit.
Referring to fig. 8 and 12, the bottle locking unit 10 includes an inner housing 101 matching with the reagent bottle, a pair of symmetrical concave shells 102 are provided on both sides of the inner housing 101, and the concave shells 102 are communicated with the inner housing 101; the tops of the inner shell 101 and the concave shell 102 are fixedly connected with a protruding sheet 103, and the protruding sheet 103 is clamped above the through hole 43; the side wall of the concave shell 102 is provided with a square opening 104; the concave shell 102 is provided with a wedge block 105 penetrating through the square opening 104, the wedge block 105 is obliquely arranged, the upper end of the wedge block 105 is obliquely arranged outwards and upwards, the other two sides of the inner shell 101 are provided with a pair of symmetrical concave grooves 106, and the concave grooves 106 are communicated with the inner shell 101; the bottle locking unit 10 locks the reagent bottle, through the limiting effect of the wedge block 105, the falling of the bottle locking unit 10 caused by vibration during transferring can be avoided, meanwhile, when the bottle locking unit 10 is taken out, the reagent bottle is taken out of the bottle locking unit 10, the protruding sheet 103 is extruded, under the cooperation of the concave groove 106, two sides of the inner shell 101 can be close face to face, the wedge block 105 is separated from the constraint of the guide opening 44, and finally the bottle locking unit 10 is taken out.
During the use, the reagent bottle is put into the bottle locking unit 10 for locking, then the bottle locking unit 10 is put into the through hole 43 on the square strip 42, the cooperation of the leak-proof baffle 5, the leak-proof ring I7 and the leak-proof ring II 8 is used for forming a dual leak-proof framework, the outside environment is prevented from influencing the inside of the reagent box, the outer baffle 6 is assembled at the outermost side of the box opening 11, thus forming a heat-insulating framework, and the reagent box has strong impact resistance and ensures the safety during the transfer of the reagent box; the touch screen 18 inputs a low temperature value and a pressure value, the first switch 36 is opened by the chip 31, the cooling system 34 works, the reagent box body 1 is cooled and reduced to the low temperature value, the temperature sensing element 35 and the pressure sensing element 17 transmit low temperature signals and pressure signals in the reagent box body 1 to the chip 31, when the pressure in the reagent box body 1 is larger than the input pressure value, the second switch 37 is opened, and then the external exhaust is carried out, so that the aim of pressure relief is achieved, after the pressure is constant, the second switch 37 is closed, the low temperature storage of mesenchymal stem cells is ensured, and the safety of the reagent box during transferring is ensured.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. The mesenchymal stem cell activity detection kit comprises a kit body and is characterized in that a kit opening is formed in the side edge of the kit body, the kit opening is communicated with the inside of the kit body, and a sealing unit is arranged at the kit opening;
the top of the kit body is provided with a cooling unit for cooling the kit body;
the upper wall surface and the lower wall surface in the kit body are respectively provided with a constraint cylinder, the two constraint cylinders are arranged opposite to each other, and a placing unit for placing a reagent bottle is connected between the two constraint cylinders;
the sealing unit comprises a leakage-proof baffle and an outer baffle; the inner wall of the box opening is fixedly provided with a square frame, a strip-shaped channel is arranged on the wall surface of the square frame facing the outside, and a leakage-proof ring I is clamped in the strip-shaped channel;
the side of the wall surface of the square frame facing the outside and the joint of the wall surface and the inner wall of the box opening are provided with a second leakage preventing ring, a magnet is arranged in the second leakage preventing ring, four side walls of the leakage preventing partition plate are provided with a third leakage preventing ring which is attracted with the second leakage preventing ring, and the magnet is arranged in the third leakage preventing ring; the outer partition plate is a hollow square plate and is arranged at the outer side of the leakage-proof partition plate, two groups of constraint pieces are fixedly connected to two sides of the outer partition plate respectively, a knob is screwed on the constraint pieces, and the knob is obliquely arranged; wedge-shaped tables which are mutually matched with the knob to restrict the outer partition plate to move are fixedly arranged on the two side walls of the box opening;
the placing unit comprises a hollow shaft and a plurality of square strips fixedly connected to the outer wall surface of the hollow shaft in a staggered manner, a plurality of through holes are formed in the square strips at equal intervals, a pair of square guide holes are formed in the inner peripheral surface of each through hole, and a bottle locking unit for locking the reagent bottle is arranged on each through hole;
the bottle body locking unit comprises an inner shell matched with the reagent bottle body, a pair of symmetrical concave shells are arranged on two sides of the inner shell, and the concave shells are communicated with the inner shell; the tops of the inner shell and the concave shell are fixedly connected with protruding sheets, and the protruding sheets are clamped above the through holes; the side wall of the concave shell is provided with a square opening; the concave shell is provided with a wedge block penetrating through the square opening, the wedge block is obliquely arranged, the other two sides of the inner shell are provided with a pair of symmetrical concave grooves, and the concave grooves are communicated with the inner shell.
2. The mesenchymal stem cell activity detection kit according to claim 1, wherein the outer wall of the leak-proof partition plate is provided with a pair of square through openings.
3. The mesenchymal stem cell activity detection kit according to claim 1, wherein the cooling unit comprises a chip, a first channel, a second channel, a cooling system, a temperature sensing element, a pressure sensing element, an energy storage box and a touch screen; the first channel passes through the top of the kit body and is communicated with the restraining cylinder above the kit body, and the second channel passes through the top of the kit body and is communicated with the kit body; the cooling system is communicated with the first channel through the first switch, and the second channel is provided with the second switch; the temperature sensing element, the pressure sensing element and the energy storage box are all fixedly connected to the top of the kit body, one ends of the temperature sensing element and the pressure sensing element penetrate into the kit body, the energy storage box, the temperature sensing element, the switch I, the switch II, the pressure sensing element and the touch screen are all electrically connected with the chip, and the touch screen is arranged on the outer wall surface of the kit body.
4. The mesenchymal stem cell activity detection kit according to claim 1, wherein a plurality of conducting ports are arranged on the wall surface of the hollow shaft at equal intervals.
5. The mesenchymal stem cell activity detection kit according to claim 1, wherein both longitudinal ends of the hollow shaft are communicated with a connecting cylinder, the diameter of the connecting cylinder is smaller than that of the hollow shaft, a telescopic piece is fixedly connected to the connecting cylinder, and the connecting cylinder is communicated with the restraining cylinder.
6. The mesenchymal stem cell activity detection kit according to claim 1, wherein an upper shell for protecting the cooling unit is fixedly connected to the top of the kit body, and a plurality of ventilation openings are formed in the peripheral wall of the upper shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310894710.2A CN116605509B (en) | 2023-07-20 | 2023-07-20 | Mesenchymal stem cell activity detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310894710.2A CN116605509B (en) | 2023-07-20 | 2023-07-20 | Mesenchymal stem cell activity detection kit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116605509A CN116605509A (en) | 2023-08-18 |
| CN116605509B true CN116605509B (en) | 2023-09-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310894710.2A Active CN116605509B (en) | 2023-07-20 | 2023-07-20 | Mesenchymal stem cell activity detection kit |
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| CN (1) | CN116605509B (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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