CN110817140A - Biological sample transfer box and biological sample transfer method - Google Patents
Biological sample transfer box and biological sample transfer method Download PDFInfo
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- CN110817140A CN110817140A CN201911146710.4A CN201911146710A CN110817140A CN 110817140 A CN110817140 A CN 110817140A CN 201911146710 A CN201911146710 A CN 201911146710A CN 110817140 A CN110817140 A CN 110817140A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/38—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
- B65D81/3802—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container in the form of a barrel or vat
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Abstract
The invention provides a biological sample transfer box and a biological sample transfer method, wherein the transfer box has a multilayer protection structure and comprises a sample protection box, an inner heat insulation box and an outer heat insulation box, wherein the sample protection box is used for internally arranging a sample container filled with a biological sample, the sample protection box is internally arranged in the inner heat insulation box with low temperature negative pressure, and the inner heat insulation box is arranged in the outer heat insulation box with a heat insulation function; the inner heat insulation box comprises an inner box body internally provided with an inner heat insulation layer and an inner box cover matched with an opening at the upper end of the inner box body, and the inner box cover is provided with a gas channel assembly communicated with the inside of the inner box body. The device is convenient to use, safe and reliable, and reduces heat exchange between the outside and the inside of the box body by arranging the double-layer heat-insulating layer; through place the ice chest in the insulation can and through setting up the inside evacuation of box in to the gas passage subassembly on the case lid, make biological sample be in low temperature negative pressure condition, prevent that the biological factor that probably has biological safety risk from diffusing to the outside, the polluted environment.
Description
Technical Field
The invention belongs to the technical field of biological sample detection auxiliary devices, and particularly relates to a biological sample transfer box and a biological sample transfer method.
Background
Biological sample detection is generally carried out in laboratories with corresponding biosafety levels according to biosafety levels and relevant regulation requirements, and prepared biological samples are inevitably required to be stored and transported. In order to ensure biological safety and prevent biological factors from diffusing and cause harm to the surrounding biological environment by aerosol, a biological safety laboratory usually adopts a negative pressure protection mode to ensure that the interior of the laboratory has relative negative pressure to the outside atmosphere, so that harmful aerosol is limited in the laboratory, and then the harmful aerosol is discharged after the biological factors are filtered by a gas high-efficiency filter. Currently, biological samples are transported, usually in a common transport box, after disinfection of the outer surface. Although the outer surface is disinfected, the risk of aerosol containing biosafety risk factors diffusing to the outside exists due to the fact that the outer surface is separated from a negative pressure environment, the common transfer box is poor in anti-falling performance, and biosafety risks in the sample transfer process are high.
The existing biological sample transfer box products also have the structure of an outer heat insulation box and an inner heat insulation box, but the purpose of arranging the inner heat insulation box is still heat insulation function, for example, a vacuum layer is arranged between two side walls of the inner heat insulation box and the outer heat insulation box, so that heat exchange is slowed down.
Biological samples are generally sensitive to temperature and need to be stored and transported at low temperature, and the common method is to place the biological samples to be transported in a common transport box, and then place ice bags in the transport box for transportation. These methods have some disadvantages, for example, it is difficult to maintain the temperature of the ice bag at a low temperature for a long time, and the transferred sample in the transfer box is in direct contact with the ice bag, causing a freezing phenomenon, etc.
Disclosure of Invention
In order to solve the problems, the invention provides a safe and reliable biological sample transfer box, which places a biological sample in a low-temperature negative-pressure environment and eliminates the risk of biological factors with safety risks diffusing in the transfer process.
The above object of the present invention is achieved by the following technical solutions:
a biological sample transfer box comprises an outer heat insulation box (2) and an inner heat insulation box (1) arranged in the outer heat insulation box in a built-in mode, wherein the inner heat insulation box (1) comprises an inner box body (11) and an inner box cover (12), the inner box body (11) is located in a low-temperature negative pressure environment, the inner box cover (12) is matched with an upper end opening of the inner box body (11), the inner box cover (12) is buckled at the upper end opening of the inner box body (11) to form a sealed box body, and a plurality of sample protection boxes (3) and a plurality of ice boxes (4) are arranged;
the inner box cover (12) is provided with a gas channel assembly (13), a vacuum pressure gauge (122) and a thermometer (123), the gas channel assembly (13) comprises a gas pipeline (132) communicated with the inside of the inner box body (11) and a pneumatic stop valve (131) connected in series on the gas pipeline, and the gas pipeline (132) is provided with a pneumatic connector (1321) used for connecting a vacuum pump or an air suction cylinder and a plug (133) used for plugging the port of the pneumatic connector (1321); the vacuum pressure gauge (122) and the thermometer (123) are both arranged in a groove of the inner box cover (12), and a pressure probe of the vacuum pressure gauge (122) and a temperature probe of the thermometer (123) are communicated with the inside of the inner box body (11) and are hermetically connected with the inner box cover (12).
In the biological sample transfer box, the inner heat insulation box (1) further comprises an inner heat insulation layer (14) arranged in the inner box body (11), a plurality of accommodating grooves (141) are formed in the inner heat insulation layer (14), the shapes and the sizes of the accommodating grooves (141) are matched with the sample protection box (3) and the ice box (4), and a sample container filled with biological samples is arranged in the sample protection box (3).
In the biological sample transfer box, the pneumatic stop valve (131) is arranged in a stop valve installation groove (124) formed in the inner box cover (12), and the pneumatic stop valve (131) is provided with a knob (1311) for opening or closing the pneumatic stop valve; the pneumatic connector (1321) is disposed at an end of the gas line (132) within the shut-off valve mounting slot (124).
In the biological sample transfer box, the peripheral edge end face of the inner box cover (12) is provided with a sealing structure, and the sealing structure comprises a groove arranged at the peripheral edge end face of the inner box cover (12) and an elastic sealing ring embedded in the groove.
In the biological sample transfer box, the inner heat insulation box (1) is of a cylindrical structure made of stainless steel, a plurality of buckle structures are arranged on the periphery of the outer wall of the joint of the inner box body (11) and the inner box cover (12) at intervals, and handles (112) are symmetrically arranged on the outer wall of the inner box body (11).
In the above-mentioned biological sample transfer box, buckle structure grabs (111) including setting up a plurality of joint spare (121) on outer wall all around of case lid 12 and a plurality of elasticity cards on outer wall all around of setting including box (11), and joint spare (121) and elasticity card are grabbed (111) one-to-one and are used in the cooperation and form a plurality of buckle structures.
In the above-mentioned biological sample transfer box, outer insulation can (2) including outer box (21) and with outer case lid (22) of outer box (21) upper end opening part articulated, outer box (21) and outer case lid (22) inside all be equipped with outer heat preservation (23) of being made by foam material or fibre porous material, outer heat preservation (23) middle part is scratched out with interior insulation can (1) assorted groove.
In the biological sample transfer box, the outer heat preservation box (2) is made of high-strength copolymerized PP materials, a plurality of reinforcing protrusions (211) are arranged on the inner wall of the outer box body (21) at intervals to serve as a keel reinforcing structure of the outer box body, grooves (231) matched with the reinforcing protrusions (211) of the outer box body (21) are formed in the periphery of the outer heat preservation layer (23), and the grooves (231) of the outer heat preservation layer (23) are embedded with the reinforcing protrusions (211) of the outer box body (21).
The invention also provides a biological sample transfer method which is operated by adopting the biological sample transfer box, and comprises the following processes:
1) firstly, checking the sealing and pressure maintaining effects of the inner heat preservation box (1);
2) packaging a biological sample meeting the preservation temperature requirement into a sample container, placing the sample container into a sample protection box (3), sequentially placing an ice box (4) and the sample protection box (3) meeting the temperature requirement into corresponding accommodating grooves (14) in an inner box body (11), and covering and buckling an inner box cover (12);
3) vacuumizing the inner box body (11) through a gas channel assembly (13) until a vacuum pressure gauge (122) displays-20 Pa to-40 Pa;
4) the outer surface of the box body of the inner heat insulation box (1) is disinfected, then the box body is sent out of a biological safety laboratory and is immediately placed into the outer heat insulation box (2);
5) after the biological sample transfer device arrives at a destination, the outer heat insulation box (2) is opened firstly, the outer surface of the box body of the inner heat insulation box (1) is disinfected and then sent into a laboratory, and whether the transfer condition required by the biological sample is met or not is judged by observing display data of a vacuum pressure gauge (122) and a thermometer (123).
In the above biological sample transport method, the process 1) specifically includes:
firstly, the inner box cover (12) is covered and buckled, the inner box body (11) is vacuumized through the gas channel assembly (13) until the vacuum pressure gauge (122) displays-40 Pa, the gas stop valve (131) is closed, the inner box body is placed for at least two hours, the vacuum pressure gauge (122) displays no obvious change, and the inner box body can be normally used; the ice box (4) for keeping the low temperature needs to be frozen for more than twelve hours under the required temperature condition.
By adopting the technical scheme, the invention has the following technical effects: the device has a simple structure, is convenient to use, is safe and reliable, reduces heat exchange between the outside and the inside of the box body by arranging the double-layer heat-insulating layer, and further delays the temperature rise speed of the inside of the box body; the ice box is placed in the inner heat insulation box, and the inner box body is vacuumized through the gas channel assembly arranged on the inner box cover, so that the biological sample is in a low-temperature negative pressure condition, and biological factors possibly having biological safety risks are prevented from diffusing to the outside to pollute the environment; the ice box and the sample protection box in the inner heat insulation box are both arranged in the accommodating groove of the inner heat insulation layer and are relatively independently arranged, so that the biological sample container is not in direct contact with the ice box, the sample container and the sample label can be prevented from being damaged, and the sample can be prevented from being frozen suddenly; the box body of the inner heat insulation box is made of corrosion-resistant stainless steel materials, and is sealed by a sealing structure, so that the inner heat insulation box can tolerate various disinfectants, is convenient for outer surface disinfection, and avoids causing safety risks such as biological diffusion.
Drawings
FIG. 1 is a schematic view of an exploded structure of a biological sample transport box according to the present invention;
FIG. 2 is a schematic view of an exploded structure of the inner incubator;
FIG. 3 is a schematic structural view of an inner cover of the inner incubator;
FIG. 4 is a cross-sectional view taken along line A-A of FIG. 3;
FIG. 5 is a schematic structural view of the inner insulating layer of the inner incubator;
FIG. 6 is a transverse sectional view of the outer incubator;
FIG. 7 is a comparison of the heat preservation effect of the biological sample transport box of the present invention and the existing single heat preservation layer biological transport device.
The reference numbers in the figures denote:
1-inner heat preservation box, 11-inner box body, 111-elastic clamping claw and 112-handle; 12-inner box cover, 121-clamping piece, 122-vacuum pressure gauge, 123-thermometer and 124-stop valve mounting groove; 13-gas channel assembly, 131-gas shut-off valve, 1311-knob; 132-gas line, 1321-pneumatic fitting; 133-plug; 14-inner insulating layer, 141-containing groove;
2-outer heat preservation box, 21-outer box, 211-reinforcing protrusion, 212-caster and 213-recording slot; 22-outer box cover, 221-label slot; 23-outer insulating layer, 231-groove; 24-outer box straps;
3-a sample protection box;
4-an ice box.
Detailed Description
The following describes the biological sample transport box and the biological sample transport method according to the present invention in detail with reference to the accompanying drawings and specific examples.
FIG. 1 is a structural example of a biological sample transport box according to the present invention. Referring to fig. 1, this biological sample transfer box has multilayer protection architecture, including sample protection box 3, interior insulation can 1 and outer insulation can 2, sample protection box 3 is used for the built-in sample container that is equipped with biological sample, and the inside shape of sample protection box 3 matches with the outside shape of sample container, plays the effect of protection sample container, and in the interior insulation can 1 of low temperature negative pressure was placed in sample protection box 3, interior insulation can 1 was placed in outer insulation can 2 that has the heat preservation function in, wherein:
the inner heat insulation box 1 is a sealed box structure, and referring to the embodiment shown in fig. 2, the inner heat insulation box comprises an inner box body 11 and an inner box cover 12, the upper end of the inner box body 11 is open, and the inner box cover 12 covers the opening of the inner box body 11 to form a sealed box body. Wherein:
referring to fig. 5, an inner heat insulation layer 14 is arranged inside the inner box body 11 to prevent heat exchange between the inside and the outside of the inner box body 11, and has heat insulation and cold insulation functions; the inner heat-insulating layer 14 can be made of porous materials such as foam materials and fiber materials, a plurality of accommodating grooves 141 are formed in the inner heat-insulating layer 14, the shapes of the accommodating grooves 141 are respectively matched with the shapes of the ice box 4 and the sample protection box 3, the ice box 4 and the sample protection box 3 are stably placed, and the ice box 4 is preferably made of low-temperature phase change cold storage materials. In one embodiment, two or three ice cases 4 are provided to ensure a low temperature environment is maintained for a long time in the inner case 11, and one or more sample protection cases 3 are provided depending on the size of the inner heat insulation case 1. Preferably, the inner case 11 is a cylindrical case having an open upper end.
Specifically, in order to facilitate carrying and moving the inner heat insulation box 1, handles 112 are symmetrically arranged on the outer wall of the inner box body 11.
Referring to fig. 3, the inner case cover 12 is a cover body that matches the inner case body 11. In this embodiment, the inner case cover 12 is an independent cover body that can be separated from the inner case body 11, a plurality of clamping members 121 are arranged around the outer wall of the inner case cover 12 at intervals, and the clamping members 121 are used in cooperation with a plurality of elastic clamping claws 111 arranged around the outer wall of the inner case body 11; the clip member 121 and the elastic clip 111 are combined to form a clip structure, and the clip structure may also be in a conventional form, which is not limited herein. Preferably, in order to ensure the sealing performance of the inner heat insulation box 1, the peripheral edge end face of the inner box cover 12 is provided with a sealing structure, the sealing structure comprises a groove arranged at the peripheral edge end face of the inner box cover 12 and an elastic sealing ring embedded in the groove, and when the inner box cover 12 is covered with the opening of the inner box body 11, the sealing structure is tightly pressed on the end face around the upper end opening of the inner box body 11 to seal the inner box body 11. Preferably, the inner case lid 12 is a cylindrical lid body having an open lower end.
Referring to fig. 3, the inner box cover 12 is provided with a gas channel assembly 13 communicated with the inside of the inner box body 11, and the gas channel assembly 13 is used for being matched with a vacuum pump to vacuumize the inside of the inner box body 11, so that a negative pressure environment is formed in the inner heat insulation box 1, and biological factors with safety risks are prevented from being diffused. As shown in fig. 4, the gas channel assembly 13 includes a gas pipeline 132 communicated with the inside of the inner box 11 and a pneumatic stop valve 131 connected in series to the gas pipeline, the pneumatic stop valve 131 is installed in a stop valve installation groove 124 formed in the inner box cover 12, and the pneumatic stop valve 131 is provided with a knob 1311 for opening and closing the pneumatic stop valve 131; the gas line 132 is provided with a pneumatic connector 1321 for connecting a vacuum pump and a plug 133 for plugging a port of the pneumatic connector 1321, and the pneumatic connector 1321 is provided at an end portion of the gas line 132 located in the shut-off valve mounting groove 124. In the process of transferring the biological sample, the pneumatic stop valve 131 is in a closed state, the plug 133 plugs the port of the pneumatic connector 1321, and the inner heat insulation box 1 is in a sealed state; when the inner heat preservation box 1 needs to be vacuumized, the plug 133 is pulled off, the pneumatic stop valve 131 is opened through the knob 1311, the vacuum pump is connected to the pneumatic connector 1321, and the interior of the inner heat preservation box 1 is vacuumized. With gas channel subassembly 13 setting on case lid 12 and not setting up including on box 11, the benefit lies in, interior box 11 need not set up the through-hole of connecting gas channel subassembly 13, has strengthened the intensity and the leakproofness of interior box 11, and interior case lid 12 sets up gas channel subassembly 13 relatively more nimble.
Preferably, the inner box 11 and the inner box cover 12 are both made of 304 stainless steel material to ensure that the inner thermal insulation box 1 working in a negative pressure environment has no obvious deformation, so that the inner thermal insulation box 1 keeps good sealing performance to eliminate the biological safety risk in the process of transferring the biological sample. Meanwhile, the inner box body 11 and the inner box cover 12 can tolerate various disinfectants, so that the outer surface disinfection is facilitated, the surface wiping disinfection can be performed, the spraying disinfection can also be performed, the disinfectant can be thoroughly transmitted out from a laboratory after the surface disinfection is performed through the disinfectant aqueduct, the surface disinfection effect is ensured, and the biological safety risk caused by the incomplete disinfection of the outer surface of the sample or the sample box is avoided.
In this embodiment, the inner lid 12 is further provided with a vacuum pressure gauge 122 and a temperature gauge 123, preferably, the vacuum pressure gauge 122 and the temperature gauge 123 are both disposed at a groove on the outer surface of the inner lid 12 to reduce the impact of external force, and a pressure probe of the vacuum pressure gauge 122 and a temperature probe of the temperature gauge 123 are communicated with the inside of the inner lid 11 and are connected with the inner lid 12 in a sealing manner for monitoring the internal pressure and temperature.
The auxiliary structures of the inner heat insulation box 1, such as the vacuum pressure gauge 122, the thermometer 123, the gas channel assembly 13 and the like, are hermetically connected with the inner box cover 12, are arranged in the groove of the inner box cover 12, and are protected by the integral structure of the inner heat insulation box 1, so that the integral anti-falling capability of the transfer box is enhanced.
Referring to fig. 1, the sample protection box 3 is disposed in the accommodating groove 141 of the inner insulating layer 14, the biological sample container containing the biological sample is disposed in the sample protection box 3, and the sample protection box 3 and the ice box 4 are disposed in different accommodating grooves 141 of the inner insulating layer 14, so that the sample container is prevented from directly contacting the ice box 4 in the inner insulating layer 1, thereby preventing the sample container and the sample label from being damaged, and preventing the sample from being frozen suddenly. The shape and size of the sample protection box 3 are matched with those of commonly used sample containers, such as a 20ml centrifuge tube, a 100ml plastic bottle, and the like, and obviously, the shapes of the sample protection box 3 and the ice box 4 are not limited, and the shape of the accommodating groove 141 is matched with that of the sample protection box 3 or the ice box 4. After the sample protection box 3 and the ice box 4 are placed in the containing groove 141 of the inner insulating layer 14, the positions are fixed due to the shape matching with the containing groove, and no obvious position change occurs. Therefore, even if the outside has sudden external force impact, the biological sample cannot be damaged, and meanwhile, the sample container is ensured to be not in direct contact with the ice box 4, so that the damage to the sample container and the sample label can be avoided, and the phenomenon that the biological sample is frozen suddenly can also be avoided.
As shown in fig. 1 and 6, the outer heat insulation box 2 includes an outer box 21 and an outer box cover 22, the outer box 21 is a box structure with an open upper end, and an outer heat insulation layer 23 matched with the outer box 21 structure is arranged in the outer box, so as to reduce heat exchange between the inside and the outside of the outer box 21 and have a heat insulation function; the outer case cover 22 is a cover body that matches the outer case 21, and an outer insulating layer 23 is also disposed in the outer case cover 22. The outer insulating layer 23 can also be made of porous materials such as foam materials and fiber materials, and grooves matched with the shape of the inner insulating box 1 can be formed in the outer insulating layer 23 in a digging mode, so that the inner insulating box 1 is stably placed in the outer insulating box 2, and the outer box cover 22 covers the opening of the outer box body 21 to form a sealed box body. In one embodiment, the outer box cover 22 is hinged to the outer box body through a pivot, a buckle structure is arranged on one side of a joint between the outer box cover 22 and the outer box body 21, which is opposite to the pivot, and the buckle structure can be the same as that of the inner heat insulation box 1, or can adopt the existing commonly used buckle structure, the outer box cover 22 and the outer box body 21 are tightly locked through the buckle structure, preferably, the buckle structure is further provided with a lock hole, the outer box body 21 and the outer box cover 22 can be locked, and the lock hole can be a coded lock or a commonly used mechanical lock; in another embodiment, the outer case cover 22 and the outer case 21 are independently disposed and separable from each other, and at this time, a plurality of fastening structures are disposed around the interface between the outer case cover 22 and the outer case 21 at intervals, so as to fasten and lock the outer case cover 22 and the outer case 21, and the fastening structures are the same as above.
In this embodiment, the outer box 21 may have a square tubular structure, a cylindrical structure, or other tubular structures, and the shape of the outer box 21 is not limited in the present invention.
Specifically, the outer box cover 22 covers the opening of the outer box 21, and the joint portion around the outer box cover is provided with a sealing structure, which may be the same as or different from the sealing structure of the inner heat insulation box 1.
Specifically, for carrying and using purposes, the bottom of the outer case 21 may be provided with casters 212, the side of the outer case 21 may be provided with a recording slot 213, and the top of the outer case cover 22 may be provided with a label slot 221 for placing a recording card or a sample label.
Preferably, the outer insulation can 2 is made of high-strength co-polymerized PP, a plurality of reinforcing protrusions 211 are arranged on the inner wall of the outer box 21 at intervals, the reinforcing protrusions 211 serve as a keel reinforcing structure of the outer box 21 to reinforce the anti-falling performance of the outer box 21, correspondingly, grooves 231 matched with the reinforcing protrusions 211 of the outer box 21 are formed in the periphery of the outer insulation layer 23, and the grooves 231 of the outer insulation layer 23 are embedded with the reinforcing protrusions 211 of the outer box 21. The outer box 21 with the keel structure can not deform under the action of normal external force, so that the biological sample can be better protected.
Specifically, longitudinal strap through holes are symmetrically formed in the opposite side surfaces of the outer box 21, and outer box straps 24 are inserted into the strap through holes. The outer box straps 24 are provided to facilitate carrying and transporting of the transfer box.
The biological sample transfer box adopts a double-layer heat preservation box body and an elastic sample protection box, so that heat conduction between the external environment and a sample can be reduced, and the speed of temperature rise of the sample is delayed; the inner box body 11 is always in a closed state in the sample transferring process, and the inner box body 11 is vacuumized through the gas channel assembly 13, so that the gas flow inside and outside the box body is blocked, and the effects of heat preservation and pressure maintaining are also achieved. The ice box 4 is made of a low-temperature phase change cold storage material, can be kept for 24-48 hours under the temperature condition required by sample temperature control, and ensures the low-temperature environment in the box body in a short time; as shown in figure 7, the heat preservation effect of the biological sample transfer box is obviously different from that of the existing single-layer heat preservation box, even if the heat preservation failure of the high-quality single heat preservation box is basically between 10h and 20h, the arrangement is shorter, and the heat preservation failure of the product is at least more than 30h and more than 40 h.
The biological sample transfer box adopts the structure to complete the safe transfer of the biological sample, and the specific transfer method comprises the following steps:
1) firstly, the sealing and pressure maintaining effects of the inner heat insulation box 1 are checked.
The method comprises the following specific steps: firstly, the inner box cover 12 is covered and buckled, the plug 133 of the gas channel assembly 13 is pulled out, the gas stop valve 131 in the gas channel assembly 13 is opened through the knob 1311, the vacuum pump or the air suction cylinder is connected to the pneumatic connector 1321, the inner box body 11 is vacuumized until the vacuum pressure gauge 122 shows-40 Pa, then the gas stop valve 131 is closed through the knob 1311, the plug 133 plugs the port of the pneumatic connector 1321, the inner box body 11 is placed for at least two hours, the pressure in the inner box body 11 does not change obviously (the vacuum pressure gauge 122 shows no obvious change), and the inner box body can be used normally; the ice box 4 for keeping the low temperature needs to be frozen for more than twelve hours under the required temperature condition;
2) putting the biological sample which is packaged into the corresponding sample container and meets the storage temperature requirement into the sample protection box 3, then putting the ice box 4 and the sample protection box 3 which meet the temperature requirement into the corresponding accommodating grooves 112 in the inner box body 11 in sequence, and covering and buckling the inner box cover 12;
3) the interior of the inner case 11 is evacuated through the gas passage assembly 13.
The method comprises the following specific steps: pulling out a plug 133 of the gas channel assembly 13, opening a gas stop valve 131 in the gas channel assembly 13, connecting a vacuum pump or an air extracting cylinder into a pneumatic connector 1321, and vacuumizing the inner box body 11 until the vacuum pressure meter 122 shows-20 Pa to-40 Pa; then, the gas stop valve 131 is closed, and the plug 133 plugs the port of the pneumatic connector 1321;
4) sterilizing the outer surface of the box body of the inner heat insulation box 1, then sending out of a biological safety laboratory, immediately loading into the outer heat insulation box 2, and then transferring the biological sample;
5) after the biological sample transfer device arrives at a destination, the outer heat insulation box 2 is firstly opened, the outer surface of the box body of the inner heat insulation box 1 is disinfected and then sent into a laboratory, the display data of the vacuum pressure gauge 122 and the thermometer 123 on the inner box cover 12 or the inner box body 11 are observed, if the pressure in the inner box body 11 is still negative pressure, and the temperature is within the range of the biological sample transfer requirement condition, the biological sample transfer is indicated to be safe and qualified, at the moment, the plug 133 is pulled out from the port of the pneumatic connector 1321, the gas stop valve 131 is opened, and after the pressure in the inner box body 11 returns to zero, the inner box cover 12 is opened, the biological sample is taken out, and the sample transfer is.
After the biological sample is safely transported, the inner heat preservation box 1 can be wholly immersed into disinfectant for sterilization and air drying, and then the biological sample can be reused.
By adopting the structure, the invention has the following beneficial effects:
(1) according to the invention, the ice box 4 is placed in the inner heat insulation box 1, and the inside of the inner heat insulation box is vacuumized, so that a biological sample is in a low-temperature negative pressure condition, and biological factors possibly having biological safety risks are prevented from diffusing to the outside to pollute the environment;
(2) the ice box 4 provides a low-temperature environment in the heat insulation box 1 within a certain time, so that the temperature condition required by the biological sample in the transfer process is ensured, and a double-layer heat insulation layer is adopted, so that the heat exchange between the outside and the inside of the box body is reduced, and the temperature rising speed of the inside of the box body is delayed;
(3) the inner heat-insulation box 1 and the outer heat-insulation box 2 are made of high-strength materials, so that the anti-falling performance is good; the internal heat-insulating layer, the sample protection box 3, the ice box 4 and the like have certain elasticity, and can play a due buffer protection role on the biological sample;
(4) the box body of the inner heat insulation box 1 is made of corrosion-resistant stainless steel materials and is sealed by a sealing structure, so that the inner heat insulation box can resist various disinfectants, is convenient for outer surface disinfection, and avoids safety risks such as biological diffusion and the like;
(5) the ice box 4 and the sample protection box 3 in the inner heat preservation box 1 are both arranged in the containing groove 141 of the inner heat preservation layer 14 and are arranged independently, so that the biological sample container and the ice box 4 are not in direct contact, the damage to the sample container and the sample label can be avoided, and the sample can be prevented from being frozen suddenly.
It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention, and that various equivalent modifications and changes may be made thereto without departing from the scope of the present invention.
Claims (10)
1. A biological sample transfer box comprises an outer heat insulation box (2) and an inner heat insulation box (1) arranged in the outer heat insulation box, and is characterized in that the inner heat insulation box (1) comprises an inner box body (11) and an inner box cover (12), wherein the inner box body (11) is located in a low-temperature negative pressure environment, the inner box cover (12) is matched with an upper end opening of the inner box body (11), the inner box cover (12) is buckled at the upper end opening of the inner box body (11) to form a sealed box body, and a plurality of sample protection boxes (3) and a plurality of ice boxes (4) are arranged in the inner heat insulation;
the inner box cover (12) is provided with a gas channel assembly (13), a vacuum pressure gauge (122) and a thermometer (123), the gas channel assembly (13) comprises a gas pipeline (132) communicated with the inside of the inner box body (11) and a pneumatic stop valve (131) connected in series on the gas pipeline, and the gas pipeline (132) is provided with a pneumatic connector (1321) used for connecting a vacuum pump or an air suction cylinder and a plug (133) used for plugging the port of the pneumatic connector (1321); the vacuum pressure gauge (122) and the thermometer (123) are both arranged in a groove of the inner box cover (12), and a pressure probe of the vacuum pressure gauge (122) and a temperature probe of the thermometer (123) are communicated with the inside of the inner box body (11) and are hermetically connected with the inner box cover (12).
2. The biological sample transfer box according to claim 1, wherein the inner heat insulation box (1) further comprises an inner heat insulation layer (14) arranged in the inner box body (11), a plurality of accommodating grooves (141) are formed in the inner heat insulation layer (14), the shape and the size of the accommodating grooves (141) are matched with those of the sample protection box (3) and the ice box (4), and the sample containers containing biological samples are arranged in the sample protection box (3).
3. The biological sample transfer cassette of claim 1 or 2, wherein the pneumatic shut-off valve (131) is mounted in a shut-off valve mounting groove (124) provided in the inner cassette cover (12), the pneumatic shut-off valve (131) being provided with a knob (1311) for opening or closing the pneumatic shut-off valve; the pneumatic connector (1321) is disposed at an end of the gas line (132) within the shut-off valve mounting slot (124).
4. The biological sample transfer box according to any one of claims 1 to 3, wherein the peripheral edge end surface of the inner box cover (12) is provided with a sealing structure comprising a groove provided at the peripheral edge end surface of the inner box cover (12) and an elastic sealing ring embedded in the groove.
5. The biological sample transfer box of any one of claims 1 to 4, wherein the inner heat insulation box (1) is a cylindrical structure made of stainless steel, a plurality of buckling structures are arranged around the outer wall of the joint of the inner box body (11) and the inner box cover (12) at intervals, and handles (112) are symmetrically arranged on the outer wall of the inner box body (11).
6. The biological sample transfer box of claim 5, wherein the snap structure comprises a plurality of snap members (121) arranged around the outer wall of the inner box cover 12 and a plurality of elastic catches (111) arranged around the outer wall of the inner box body (11), and the snap members (121) and the elastic catches (111) are in one-to-one correspondence and cooperate to form a plurality of snap structures.
7. The biological sample transfer box according to any one of claims 1 to 6, wherein the outer heat preservation box (2) comprises an outer box body (21) and an outer box cover (22) hinged to an opening at the upper end of the outer box body (21), an outer heat preservation layer (23) made of foam material or fiber porous material is arranged inside each of the outer box body (21) and the outer box cover (22), and a groove matched with the inner heat preservation box (1) is formed in the middle of the outer heat preservation layer (23).
8. The biological sample transfer box of claim 7, wherein the outer heat insulation box (2) is made of high-strength co-polymerized PP material, a plurality of reinforcing protrusions (211) are arranged on the inner wall of the outer box body (21) at intervals to serve as a keel reinforcing structure of the outer box body, grooves (231) matched with the reinforcing protrusions (211) of the outer box body (21) are arranged on the periphery of the outer heat insulation layer (23), and the grooves (231) of the outer heat insulation layer (23) are embedded with the reinforcing protrusions (211) of the outer box body (21).
9. A biological sample transport method operating with the biological sample transport box according to any one of claims 1 to 8, comprising the processes of:
1) firstly, checking the sealing and pressure maintaining effects of the inner heat preservation box (1);
2) packaging a biological sample meeting the preservation temperature requirement into a sample container, placing the sample container into a sample protection box (3), sequentially placing an ice box (4) and the sample protection box (3) meeting the temperature requirement into corresponding accommodating grooves (14) in an inner box body (11), and covering and buckling an inner box cover (12);
3) vacuumizing the inner box body (11) through a gas channel assembly (13) until a vacuum pressure gauge (122) displays-20 Pa to-40 Pa;
4) the outer surface of the box body of the inner heat insulation box (1) is disinfected, then the box body is sent out of a biological safety laboratory and is immediately placed into the outer heat insulation box (2);
5) after the biological sample transfer device arrives at a destination, the outer heat insulation box (2) is opened firstly, the outer surface of the box body of the inner heat insulation box (1) is disinfected and then sent into a laboratory, and whether the transfer condition required by the biological sample is met or not is judged by observing display data of a vacuum pressure gauge (122) and a thermometer (123).
10. The method for transporting a biological sample according to claim 9, wherein the process 1) comprises in particular:
firstly, the inner box cover (12) is covered and buckled, the inner box body (11) is vacuumized through the gas channel assembly (13) until the vacuum pressure gauge (122) displays-40 Pa, the gas stop valve (131) is closed, the inner box body is placed for at least two hours, the vacuum pressure gauge (122) displays no obvious change, and the inner box body can be normally used; the ice box (4) for keeping the low temperature needs to be frozen for more than twelve hours under the required temperature condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201911146710.4A CN110817140A (en) | 2019-11-21 | 2019-11-21 | Biological sample transfer box and biological sample transfer method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911146710.4A CN110817140A (en) | 2019-11-21 | 2019-11-21 | Biological sample transfer box and biological sample transfer method |
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| CN110817140A true CN110817140A (en) | 2020-02-21 |
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Application publication date: 20200221 |