CN116600661A - Composition for promoting hair growth and/or inhibiting hair loss - Google Patents

Composition for promoting hair growth and/or inhibiting hair loss Download PDF

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Publication number
CN116600661A
CN116600661A CN202180079360.3A CN202180079360A CN116600661A CN 116600661 A CN116600661 A CN 116600661A CN 202180079360 A CN202180079360 A CN 202180079360A CN 116600661 A CN116600661 A CN 116600661A
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China
Prior art keywords
extract
group
broccoli
avocado
sitosterol
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CN202180079360.3A
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Chinese (zh)
Inventor
李康宪
鞠敏
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Astor Rogens Co ltd
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Astor Rogens Co ltd
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Priority claimed from PCT/KR2021/013668 external-priority patent/WO2022075731A1/en
Publication of CN116600661A publication Critical patent/CN116600661A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/03—Organic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/23—Removal of unwanted matter, e.g. deodorisation or detoxification by extraction with solvents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00—Processes
    • A23V2300/14—Extraction

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

The present application relates to a composition for promoting hair growth and/or preventing, alleviating or treating alopecia, comprising herbal extracts and/or effective compounds as active ingredients.

Description

Composition for promoting hair growth and/or inhibiting hair loss
Cross reference to
The present application requests priority from korean patent application No. 10-2020-0129117, which was filed on 7 th 10 th of 2020, and korean patent application No. 10-2020-0129118, which was filed on 7 th 10 th of 2020, and the entire contents disclosed in the corresponding korean patent application documents are incorporated herein as part of the specification.
Technical Field
The present application relates to a composition for promoting hair growth and/or inhibiting hair loss, which comprises herbal extracts and/or active compounds as active ingredients.
Background
The human head originates from hair follicles present in the scalp. Since hair follicles are initially formed in fetuses and are no longer produced after birth, the number of hair follicles remains unchanged from puberty to adulthood, and then gradually decreases from the elderly. The human hair repeatedly grows and kills through the unique cycle of the anagen phase, the catagen phase and the telogen phase. This hair cycle is repeated for 3 to 6 years and is known to be affected by various factors such as age, season, disease, stress, and the like, in addition to genetic factors. In general, baldness refers to an abnormal increase in the number of hair loss during the aforementioned hair cycle due to an increase in the number of hairs in the withdrawal or resting phase and a decrease in the number of hairs in the growing phase at the same time.
In general, alopecia is caused by heredity, aging, diseases, medication, radiotherapy, etc., but from a biochemical or physiological point of view, the cause of alopecia is androgen hypersecretion, sebum hypersecretion, scalp blood circulation disorder, nutrient deficiency, nutrient imbalance, stress, etc. However, the exact cause and mechanism of hair loss has not been determined, and there is also a shortage of definitive methods or medicaments for treating alopecia.
Disclosure of Invention
[ technical problem ]
An embodiment of the present invention provides a composition for promoting hair growth, comprising one or more selected from the group consisting of sulforaphane, indole-3-methanol, β -sitosterol (β -sitosterol), and salts thereof as an active ingredient.
Another embodiment provides a composition for promoting hair growth, comprising broccoli (broccoli) extract, avocado (avocado) extract, or a mixture thereof as an active ingredient.
The composition for promoting hair growth may be a pharmaceutical composition, a cosmetic composition, and/or a food composition (e.g., a health functional food).
Other embodiments provide a pharmaceutical composition for preventing, alleviating or treating alopecia, comprising one or more selected from the group consisting of sulforaphane, indole-3-methanol, beta-sitosterol (beta-sitosterol) and salts thereof as an active ingredient.
Other embodiments provide pharmaceutical compositions for preventing, alleviating or treating hair loss comprising broccoli extract, avocado extract, or mixtures thereof as an active ingredient.
Other embodiments provide cosmetic compositions for preventing, alleviating or treating hair loss comprising one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, beta-sitosterol (beta-sitosterol), and salts thereof as an active ingredient.
Other embodiments provide cosmetic compositions for preventing, alleviating or treating hair loss comprising broccoli extract, avocado extract, or mixtures thereof as an active ingredient.
Other embodiments provide a food composition for preventing, alleviating or treating alopecia, comprising one or more of sulforaphane, indole-3-methanol, beta-sitosterol, and salts thereof as an active ingredient. The food composition can be a health functional food.
Other embodiments provide a food composition for preventing, alleviating or treating hair loss comprising broccoli extract, avocado extract or a mixture thereof as an active ingredient. The food composition may be a health functional food.
Other embodiments provide methods for promoting hair growth and/or preventing, alleviating and/or treating hair loss comprising administering to a subject in need of promoting hair growth and/or preventing, alleviating and/or treating hair loss an effective amount of one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, beta-sitosterol, and salts thereof.
Other embodiments provide methods for promoting hair growth and/or preventing, alleviating and/or treating hair loss comprising administering to a subject in need of promoting hair growth and/or preventing, alleviating and/or treating hair loss an effective amount of an extract of broccoli, an extract of avocado, or a mixture thereof.
Other embodiments provide for the use of one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, β -sitosterol and salts thereof for promoting hair growth and/or preventing, alleviating or treating hair loss; or for the preparation of a composition for promoting hair growth and/or preventing, alleviating or treating hair loss.
Other embodiments provide for the use of broccoli extract, avocado extract, or mixtures thereof for promoting hair growth and/or preventing, alleviating, and/or treating hair loss; or for the preparation of a composition for promoting hair growth and/or preventing, alleviating and/or treating hair loss.
[ means of technology ]
Herein, herbal extracts and useful compounds having effects of promoting hair growth and/or inhibiting hair loss are identified, thereby providing their use in promoting hair growth and/or inhibiting hair loss.
More specifically, an embodiment provides a composition for promoting hair growth, comprising one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, β -sitosterol (β -sitosterol), and salts thereof as an active ingredient.
Another embodiment provides a composition for promoting hair growth, comprising broccoli (broccoli) extract, avocado (avocado) extract, or a mixture thereof as an active ingredient.
The composition for promoting hair growth may be a pharmaceutical composition, a cosmetic composition, and/or a food composition (e.g., a health functional food).
Other embodiments provide a pharmaceutical composition for preventing, alleviating or treating alopecia, comprising one or more selected from the group consisting of sulforaphane, indole-3-methanol, β -sitosterol and salts thereof as an active ingredient.
Other embodiments provide a pharmaceutical composition for preventing, alleviating or treating alopecia comprising broccoli extract, avocado extract or a mixture thereof as an active ingredient.
Other embodiments provide cosmetic compositions for preventing, alleviating or treating hair loss comprising as an active ingredient one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, β -sitosterol and salts thereof.
Other embodiments provide cosmetic compositions for preventing, alleviating or treating hair loss comprising broccoli extract, avocado extract, or mixtures thereof as an active ingredient.
Other embodiments provide a food composition for preventing, alleviating or treating alopecia, comprising one or more selected from the group consisting of sulforaphane, indole-3-methanol, β -sitosterol and salts thereof as an active ingredient. The food composition can be a health functional food.
Other embodiments provide a food composition for preventing, alleviating or treating hair loss comprising broccoli extract, avocado extract or a mixture thereof as an active ingredient. The food composition can be a health functional food.
Other embodiments provide methods for promoting hair growth and/or preventing, alleviating and/or treating hair loss comprising administering to a subject in need of promoting hair growth and/or preventing, alleviating and/or treating hair loss an effective amount of one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, beta-sitosterol, and salts thereof.
Other embodiments provide methods for promoting hair growth and/or preventing, alleviating and/or treating hair loss comprising administering to a subject in need of promoting hair growth and/or preventing, alleviating and/or treating hair loss an effective amount of an extract of broccoli, an extract of avocado, or a mixture thereof.
Other embodiments provide for the use of one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, β -sitosterol and salts thereof for promoting hair growth and/or preventing, alleviating or treating hair loss; or for the preparation of a composition for promoting hair growth and/or preventing, alleviating or treating hair loss.
Other embodiments provide for the use of broccoli extract, avocado extract, or mixtures thereof for promoting hair growth and/or preventing, alleviating, and/or treating hair loss; or for the preparation of a composition for promoting hair growth and/or preventing, alleviating and/or treating hair loss.
Detailed Description
All values or ranges of values associated with the compositions and methods provided herein can be selected to contribute to or exhibit more beneficial effects for the purposes of the compositions and methods, such as promoting hair growth and/or inhibiting hair loss (preventing, alleviating, and/or treating hair loss).
Hereinafter, the present invention will be described in more detail.
Active compounds
Sulforaphane (SFN) is of formula C 6 H 11 NOS 2 (CAS number 4478-93-7), and may have the structure of formula 1 below.
[ chemical formula 1]
Indole-3-carbanol (I3C) is of formula C 9 H 9 A compound of NO (CAS No. 700-06-1) and may have the structure of the following chemical formula 2.
[ chemical formula 2]
Beta-sitosterol is of formula C 29 H 50 O (CAS number 83-46-5), and may have the structure of formula 3 below.
[ chemical formula 3]
The compounds of chemical formulas 1 to 3 or salts thereof may be obtained by extraction and/or isolation from natural products and/or varieties, or may be prepared by a conventional organic synthesis method, but are not limited thereto.
In the present application, "salt of a compound" may mean a physiologically acceptable salt among salts, which is a substance in which cations and anions are combined by electrostatic attraction, and for example, may mean a pharmaceutically acceptable salt, a cosmetically acceptable salt, and/or a salt acceptable for food. For example, the salt may be one or more salts selected from the group consisting of: metal salts, salts with organic bases, salts with inorganic acids, salts with organic acids, salts with basic or acidic amino acids, and the like. In one embodiment, the metal salt may be one or more metal salts selected from the group consisting of: alkali metal salts (sodium salt, potassium salt, etc.), alkaline earth metal salts (potassium salt, magnesium salt, barium salt, etc.), aluminum salts, etc.; the salt with the organic base may be one or more salts selected from the group consisting of: salts with triethylamine, pyridine, picoline, 2, 6-lutidine, ethanolamine, diethanolamine, triethanolamine, cyclohexylamine, dicyclohexylamine, N-dibenzylethylenediamine, and the like; the salt with the mineral acid may be one or more salts selected from the group consisting of: salts with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, phosphoric acid, and the like; the salt with the organic acid may be one or more salts selected from the group consisting of: salts of formic acid, acetic acid, trifluoroacetic acid, phthalic acid, fumaric acid, oxalic acid, tartaric acid, maleic acid, citric acid, succinic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, and the like; the salt with a basic amino acid may be one or more salts selected from the group consisting of: salts with arginine, lysine, ornithine and the like; and the salt with an acidic amino acid may be one or more salts selected from the group consisting of: salts with aspartic acid, glutamic acid, etc.
In one embodiment, the active ingredient may be sulforaphane.
In another embodiment, the active ingredient may be indole-3-methanol.
In other embodiments, the active ingredient may be β -sitosterol.
In other embodiments, the active ingredient may be a combination of sulforaphane and indole-3-methanol. In this case, the ratio of raphanin to indole-3-methanol contained in the compositions provided herein or administered in the methods provided herein may be 10 by weight: (0.1-10), 10: (0.1-7.5), 10: (0.1-5), 10: (0.1-2.5), 10: (0.1-2), 10: (0.1-1.5), 10: (0.1-1.25), 10: (0.25-10), 10: (0.25-7.5), 10: (0.25-5), 10: (0.25-2.5), 10: (0.25-2), 10: (0.25-1.5), 10: (0.25-1.25), 10: (0.5-10), 10: (0.5-7.5), 10: (0.5-5), 10: (0.5-2.5), 10: (0.5-2), 10: (0.5-1.5), 10: (0.5-1.25), 10: (0.75-10), 10: (0.75-7.5), 10: (0.75-5), 10: (0.75-2.5), 10: (0.75-2), 10: (0.75-1.5), 10: (0.75-1.25), 10: (1-10), 10: (1-7.5), 10: (1-5), 10: (1-2.5), 10: (1-2), 10: (1-1.5), 10: (1-1.25), 10: (1.25-10), 10: (1.25-7.5), 10: (1.25-5), 10: (1.25-2.5), 10: (1.25-2), 10: (1.25-1.5), 10: (2.5-10), 10: (2.5-7.5), 10: (2.5-5), about 10:1.25, or about 10:2.5 (weight of sulforaphane: weight of indole-3-methanol to date).
In other embodiments, the active ingredient may be a combination of sulforaphane and β -sitosterol. In this case, the ratio of sulforaphane to β -sitosterol contained in the compositions provided herein or administered in the methods provided herein may be 10 by weight: (0.1-10), 10: (0.1-7.5), 10: (0.1-5), 10: (0.1-2.5), 10: (0.1-2), 10: (0.1-1.5), 10: (0.1-1.25), 10: (0.25-10), 10: (0.25-7.5), 10: (0.25-5), 10: (0.25-2.5), 10: (0.25-2), 10: (0.25-1.5), 10: (0.25-1.25), 10: (0.5-10), 10: (0.5-7.5), 10: (0.5-5), 10: (0.5-2.5), 10: (0.5-2), 10: (0.5-1.5), 10: (0.5-1.25), 10: (0.75-10), 10: (0.75-7.5), 10: (0.75-5), 10: (0.75-2.5), 10: (0.75-2), 10: (0.75-1.5), 10: (0.75-1.25), 10: (1-10), 10: (1-7.5), 10: (1-5), 10: (1-2.5), 10: (1-2), 10: (1-1.5), 10: (1-1.25), 10: (1.25-10), 10: (1.25-7.5), 10: (1.25-5), 10: (1.25-2.5), 10: (1.25-2), 10: (1.25-1.5), 10: (2.5-10), 10: (2.5-7.5), 10: (2.5-5), about 10:1.25, or about 10:2.5 (weight of sulforaphane: weight of beta-sitosterol so far).
In other embodiments, the active ingredient may be a combination of indole-3-methanol and β -sitosterol. In this case, the ratio of indole-3-methanol to β -sitosterol contained in the compositions provided herein or administered in the methods provided herein may be 1 by weight: (0.1-10), 1: (0.1 to 7.5), 1: (0.1-5), 1: (0.1-2.5), 1: (0.1-1), 1: (0.25-10), 1: (0.25-7.5), 1: (0.25-5), 1: (0.25-2.5), 1: (0.25-1), 1: (0.5-10), 1: (0.5-7.5), 1: (0.5-5), 1: (0.5-2.5), 1: (0.5-1), 1: (0.75-10), 1: (0.75-7.5), 1: (0.75-5), 1: (0.75-2.5), 1: (0.75-1), 1: (1-10), 1: (1-7.5), 1: (1-5), 1: (1-2.5), or about 1:1 (weight of indole-3-methanol: weight of beta-sitosterol to date).
In other embodiments, the active ingredient may be a combination of sulforaphane, indole-3-methanol, and β -sitosterol. In this case, the ratio of raphanin, indole-3-methanol, to β -sitosterol contained in the compositions provided herein or administered in the methods provided herein may be 10 by weight: (0.1-10): (0.1-10), 10: (0.1-7.5): (0.1-7.5), 10: (0.1-5): (0.1-5), 10: (0.1-2.5): (0.1-2.5), 10: (0.1-2): (0.1-2), 10: (0.1-1.5): (0.1-1.5), 10: (0.1-1.25): (0.1-1.25), 10: (0.25-10): (0.25-10), 10: (0.25-7.5): (0.25-7.5), 10: (0.25-5): (0.25-5), 10: (0.25-2.5): (0.25-2.5), 10: (0.25-2): (0.25-2), 10: (0.25-1.5): (0.25-1.5), 10: (0.25-1.25): (0.25-1.25), 10: (0.5-10): (0.5-10), 10: (0.5-7.5): (0.5-7.5), 10: (0.5-5): (0.5-5), 10: (0.5-2.5): (0.5-2.5), 10: (0.5-2): (0.5-2), 10: (0.5-1.5): (0.5-1.5), 10: (0.5-1.25): (0.5-1.25), 10: (0.75-10): (0.75-10), 10: (0.75-7.5): (0.75-7.5), 10: (0.75-5): (0.75-5), 10: (0.75-2.5): (0.75-2.5), 10: (0.75-2): (0.75-2), 10: (0.75-1.5): (0.75-1.5), 10: (0.75-1.25): (0.75-1.25), 10: (1-10): (1-10), 10: (1-7.5): (1-7.5), 10: (1-5): (1-5), 10: (1-2.5): (1-2.5), 10: (1-2): (1-2), 10: (1-1.5): (1-1.5), 10: (1-1.25): (1-1.25), 10: (1.25-10): (1.25-10), 10: (1.25-7.5): (1.25-7.5), 10: (1.25-5): (1.25-5), 10: (1.25-2.5): (1.25-2.5), 10: (1.25-2), 10: (1.25-1.5): (1.25 to 1.5), or 10:1.25:1.25 (weight of sulforaphane: weight of indole-3-methanol: weight of beta-sitosterol to date).
In one embodiment, the raphanin, indole-3-methanol, β -sitosterol, and/or salts thereof described as active ingredients in the compositions provided herein and/or methods provided herein may be contained in or isolated (or purified) from Broccoli (broccolii) extract, avocado extract, or mixtures thereof.
Extract of plant
Broccoli (Brassica oleracea var. Italica) is an annual dicotyledon belonging to the family Brassicaceae of Papaverales and is used in various ways for food. The broccoli extract used herein may be obtained by using one or more parts selected from the group consisting of broccoli seeds, buds, flowers, roots, whole plants, and the like. A shoot may refer to a first stem and/or leaf produced from a seed. The broccoli used herein for extraction may be in the form of a raw material, a dried product, or a crushed product of herbal or dried products, but is not limited thereto.
Avocado (Persea americana)) is an annual dicotyledonous plant belonging to the camphoriaceae family of camphorales, and is a fruit native to mexico and central america. Avocado extract as used herein may be obtained by using one or more parts selected from the group consisting of avocado seeds, fruits (flesh and/or pericarp), buds, flowers, stems, roots, whole plants, and the like. A shoot may refer to a first stem and/or leaf produced from a seed. Avocados for extraction herein can be in the form of raw materials, dried products, or crushed products of herbal or dried products, but are not limited thereto.
The extract of broccoli may be obtained by extracting broccoli with one or more extraction solvents selected from the group consisting of water and a linear or branched alcohol having 1 to 4 carbon atoms. In one embodiment of the present invention, in one embodiment, the extract of broccoli may be prepared by using water, or 5 to 100% (v/v), 5 to 98% (v/v), 5 to 95% (v/v), 5 to 92% (v/v), 5 to 90% (v/v), 5 to 85% (v/v), 5 to 80% (v/v), 5 to 75% (v/v), 5 to 70% (v/v), 5 to 65% (v/v), 5 to 60% (v/v), 5 to 55% (v/v), 5 to 50% (v/v), 5 to 45% (v/v), 5 to 40% (v/v), 5 to 35% (v/v), 5 to 30% (v/v), 5 to 25% (v/v), 5 to 20% (v/v), 5 to 15% (v/v), 5 to 10% (v/v), 10 to 100% (v/v), 10 to 98% (v/v), 10 to 95% (v/v), 10 to 92% (v/v), 10 to 90% (v/v), 10 to 85% (v, 10 to 80% (v), 10 to 80% (v/v), 5 to 35% (v/v, 5 to 35% (v), 5 to 30% (v/v), 5% (v/v), 10-60% (v/v), 10-55% (v/v), 10-50% (v/v), 10-45% (v/v), 10-40% (v/v), 10-35% (v/v), 10-30% (v/v), 10-25% (v/v), 10-20% (v/v), 10-15% (v/v), 15-100% (v/v), 15-98% (v/v), 15-95% (v/v), 15-92% (v/v), 15-90% (v/v), 15-85% (v/v), 15-80% (v/v), 15-75% (v/v), 15-70% (v/v), 15-65% (v/v), 15-60% (v/v), 15-55% (v/v), 15-50% (v/v), 15-45% (v), 15-40% (v/v), 15-35% (v/v), 15-30% (v/v), 15-25% (v), 15-20% (v/v), 15-75% (v/v), 15-20% (v/v), 15-70% (v/v), 15-65% (v/v), 20-90% (v/v), 20-85% (v/v), 20-80% (v/v), 20-75% (v/v), 20-70% (v/v), 20-65% (v/v), 20-60% (v/v), 20-55% (v/v), 20-50% (v/v), 20-45% (v/v), 20-40% (v/v), 20-35% (v/v), 20-30% (v/v), 20-25% (v/v), 25-100% (v/v), 25-98% (v/v), 25-95% (v/v), 25-92% (v/v), 25-90% (v/v), 25-85% (v/v), 25-80% (v/v), 25-75% (v/v), 25-70% (v/v), 25-65% (v), 25-60% (v/v), 25-55% (v/v), 25-50% (v/v), 25-45% (v), 25-40% (v), 25-35% (v/v), 25-92% (v/v), 25-30% (v) 100% (v/v), 25-90% (v/v), 25-80% (v/v), 25-75% (v) and 30% (v/v) 30 (v), 30-95% (v/v), 30-28% (v/v), 30-90% (v/v), 30-85% (v/v), 30-80% (v/v), 30-75% (v/v), 30-70% (v/v), 30-65% (v/v), 30-60% (v/v), 30-55% (v/v), 30-50% (v/v), 30-45% (v/v), 30-40% (v/v), 30-35% (v/v), 35-100% (v/v), 35-98% (v/v), 35-95% (v/v), 35-92% (v/v), 35-90% (v/v), 35-85% (v/v), 35-80% (v/v), 35-75% (v/v), 35-70% (v/v), 35-65% (v), 35-60% (v/v), 35-55% (v/v), 35-50% (v/v), 35-45% (v), 35-40% (v/v), 35-95% (v/v), 35-92% (v/v), 35-40% (v/v) and 40% (v/v), 40-90% (v/v), 40-85% (v/v), 40-80% (v/v), 40-75% (v/v), 40-70% (v/v), 40-65% (v/v), 40-60% (v/v), 40-55% (v/v), 40-50% (v/v), 40-45% (v/v), 45-100% (v/v), 45-98% (v/v), 45-95% (v/v), 45-92% (v/v), 45-90% (v/v), 45-85% (v/v), 45-80% (v/v), 45-75% (v/v), 45-70% (v/v), 45-65% (v/v), 45-60% (v/v), 45-55% (v/v), 45-50% (v/v), 50-100% (v), 50-98% (v/v), 50-95% (v/v), 50-92% (v/v), 50-90% (v), 50-85% (v/v), 50-80% (v), 50-75% (v/v), 50-70% (v) 50-50% (v) and 50-50% (v), 50-60% (v/v), 50-55% (v/v), 55-100% (v/v), 55-98% (v/v), 55-95% (v/v), 55-92% (v/v), 55-90% (v/v), 55-85% (v/v), 55-80% (v/v), 55-75% (v/v), 55-70% (v/v), 55-65% (v/v), 55-60% (v/v), 60-100% (v/v), 60-98% (v/v), 60-95% (v/v), 60-92% (v/v), 60-90% (v/v), 60-85% (v/v), 60-80% (v/v), 60-75% (v/v), 60-70% (v/v), 60-65% (v/v), 65-100% (v), 65-98% (v/v), 65-95% (v/v), 65-92% (v/v), 65-90% (v), 65-85% (v/v), 65-90% (v), 65% (v/v), 65-70% (v) and 65% (v/v), 70 to 98% (v/v), 70 to 95% (v/v), 70 to 92% (v/v), 70 to 90% (v/v), 70 to 85% (v/v), 70 to 80% (v/v), 70 to 75% (v/v), 75 to 100% (v/v), 75 to 98% (v/v), 75 to 95% (v/v), 75 to 92% (v/v), 75 to 90% (v/v), 75 to 85% (v/v), 75 to 80% (v/v), 80 to 100% (v/v), 80 to 98% (v/v), 80 to 95% (v/v), 80 to 92% (v/v), 80 to 90% (v/v), 80 to 85% (v/v), 85 to 100% (v/v), 85 to 98% (v/v), 85 to 95% (v/v), 85 to 92% (v/v), 85 to 90% (v/v), 90 to 98% (v/v) and alcohol-extracted cerulosa flowers, and/or can pass through the flow passage of 30 to 75, 30 to 70, 30 to 65, 30 to 60, 30 to 50, 30 to 40, 40 to 100, 40 to 900, 40 to 800, 40 to 750, 40 to 700, 40 to 650, 40 to 600, 40 to 500, 50 to 100, 50 to 900, 50 to 800, 50 to 750, 50 to 700, 50 to 650, 50 to 600, 60 to 100, 60 to 900, 60 to 800, 60 to 750, 60 to 700, 60 to 650, 65 to 100, 65 to 900, 65 to 800, 65 to 750, 65 to 700, 70 to 100, 70 to 900, 70 to 800, 70 to 750, 75 to 100, 75 to 900, 75 to 800, 80 to 100, 80 to 900 or 90 to 100.
Avocado extract can be obtained by extracting avocado with an organic solvent, such as petroleum ether (CAS No. 101316-46-5).
The extraction time for extracting the broccoli and/or avocado is not limited as long as it is sufficient for extraction, and may be set to about 1 hour or more, 2 hours or more, 3 hours or more, 4 hours or more, for example, 1 to 24 hours, 2 to 24 hours, 3 to 24 hours, 4 to 24 hours, 1 to 12 hours, 2 to 12 hours, 3 to 12 hours, 4 to 12 hours, 1 to 6 hours, 2 to 6 hours, 3 to 6 hours, or 4 to 6 hours.
The extraction procedure for obtaining the broccoli extract and/or the avocado extract may be carried out by all usual extraction methods, for example, by one or more methods selected from the group consisting of hot water extraction, ultrasonic extraction reflux extraction, and the like, but is not limited thereto. After the extraction procedure, any extract may further be dried and/or concentrated by conventional methods.
The extraction may be performed one or two or more times (e.g., two, three, four, or five times).
The mixing ratio of the broccoli extract to the avocado extract in the extract mixture may be 1 by solid weight: 0.1-10, 1 (0.1-5), 1 (0.1-3), 1 (0.1-2.5), 1 (0.1-2), 1 (0.1-1.5), 1 (0.1-1), 1 (0.1-0.67), 1 (0.1-0.5), 1 (0.1-0.4), 1 (0.1-0.33), 1 (0.1-0.25), 1 (0.1-0.2), 1 (0.2-10), 1 (0.2-5), 1 (0.2-4), 1 (0.2-2.5), 1 (0.2-1.5), 1 (0.2-1.2), 1 (0.2-1), 1 (0.67), 1 (0.2-0.5), 1 (0.2-0.4), 1 (0.2-0.33), 1-0.2, 1 (0.25), 1-25, 1 (0.25) and 25 (0.25) 0.1-25) 0.25.1-25 1 (0.33-4), 1 (0.33-3), 1 (0.33-2.5), 1 (0.33-2), 1 (0.33-1.5), 1 (0.33-1), 1 (0.33-0.67), 1 (0.33-0.5), 1 (0.33-0.4), 1 (0.4-10), 1 (0.4-5), 1 (0.4-4), 1 (0.4-3), 1 (0.4-2.5), 1 (0.4-2), 1 (0.4-1.5), 1 (0.4-1), 1 (0.67), 1 (0.4-0.5), 1 (0.5-10), 1 (0.5-5), 1 (0.5-4), 1 (0.5-3), 1 (0.5-2.5), 1 (0.5-2), 1.5-1.5), 1 (0.5-5) or 1.67 by weight of the extract of the crocodile flower solid. The solid weight means the weight of the solid remaining after the solvent component of the extract is removed. This is a term for indicating that the mixing ratio means a ratio between weights of active ingredients from which the extraction solvent is removed.
The broccoli extract, avocado extract, or mixtures thereof described herein may be in the form of an extract (crude solution), a concentrate of the extract, or a dried product of the extract or concentrate.
The extract may comprise one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, beta-sitosterol and salts thereof.
Use of the same
The active ingredients (compounds and/or extracts), compositions comprising the active ingredients, and methods of using the active ingredients provided herein may have the effect of promoting hair growth (or hair growth promotion) and/or inhibiting (preventing, alleviating, and/or treating) hair loss.
Herein, "hair growth" refers to all actions that induce and/or promote hair production and/or growth, while "hair loss" refers to all states of reduced hair. The hair cycle may be divided into a growing period of hair growth, a regressing period of hair bulb stopping growth and contraction, a resting period of old hair dehairing by generating new hair, and a new growing period of hair with the new hair generated as a final period of the resting period. The hair may have periodicity by repeating the above-described neonatal, anagen, catagen, and telogen phases, and the telogen phase may be prolonged when symptoms of alopecia occur.
Herein, "treatment" is intended to encompass reducing, ameliorating, and alleviating symptoms (alopecia), reducing the scope of symptoms, delaying the progression of symptoms, and the like, or any one or more selected from the group. "preventing" is intended to cover all mechanisms and/or effects acting on a subject without symptoms (alopecia) to prevent the appearance of symptoms or delay the age of onset. By "ameliorating or alleviating" may be meant at least reducing a parameter associated with a disorder in which a symptom is treated by administration of a composition according to one embodiment, such as the severity of the symptom.
The subjects to which the compositions (pharmaceutical, cosmetic and/or food compositions) and/or active ingredients (active compounds and/or extracts) provided herein are administered may be mammals, including humans, dogs, cats, horses, cows, pigs, goats, rabbits, mice, rats, etc., e.g., humans.
In the pharmaceutical compositions provided herein or methods related thereto, the administration or method of administration of the compositions and/or active ingredients may be any of the usual methods, and may be, for example, oral administration, or parenteral administration such as dermal (e.g., scalp) administration, transdermal administration (through the scalp). The composition may be used by formulating into oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., or non-oral formulations such as suspensions, emulsions, aerosols, ointments, patches, gels, etc., according to common methods.
In addition to containing the active ingredient, the pharmaceutical compositions provided herein may further contain adjuvants such as pharmaceutically and/or physiologically acceptable carriers, excipients, and/or diluents, and the like. Examples of carriers, excipients or diluents may include one or more substances selected from the group consisting of: lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, mineral oil and the like. In the formulation, one or more diluents or excipients selected from the group consisting of usual fillers, thickeners, binders, wetting agents, disintegrants, surfactants, etc. may be used. Solid formulations for oral administration comprise one or more substances selected from the group consisting of: tablets, pills, powders, granules, capsules, syrups, powders, suspensions, and the like, and such solid formulations may be prepared by mixing at least one or more excipients, for example one or more excipients selected from the group consisting of starch, calcium carbonate, sucrose, lactose, gelatin, and the like, into the extract. In addition, in addition to using simple excipients, lubricants such as magnesium stearate talc are also used. One or more selected from the group consisting of suspensions, oral liquids, emulsions, syrups and the like corresponds to a liquid formulation for oral administration, and may contain various excipients, for example, one or more selected from wetting agents, sweeteners, fragrances, preservatives and the like, in addition to usual simple diluents, water or liquid paraffin. Formulations for parenteral administration include one or more members selected from the group consisting of sterile aqueous solutions, nonaqueous solutions, suspensions, emulsions, lyophilized formulations, transdermal formulations, and the like. As non-aqueous solutions or suspensions, one or more selected from propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate may be used.
The content of the active ingredient (active compound (sulforaphane, indole-3-methanol, beta-sitosterol, or a combination thereof) and/or extract (solid)) contained in the pharmaceutical composition can be appropriately determined depending on the form of formulation, desired effect, etc., for example, the total weight of the pharmaceutical composition may be 0.0001 to 99 wt%, 0.0001 to 90 wt%, 0.0001 to 80 wt%, 0.0001 to 70 wt%, 0.0001 to 60 wt%, 0.0001 to 50 wt%, 0.0001 to 40 wt%, 0.0001 to 30 wt%, 0.0001 to 20 wt%, 0.0001 to 10 wt%, 0.01 to 99 wt%, 0.01 to 90 wt%, 0.01 to 80 wt%, 0.01 to 70 wt%, 0.01 to 60 wt%, 0.01 to 50 wt%, 0.01 to 40 wt%, 0.01 to 30 wt%, 0.01 to 20 wt%, 0.01 to 10 wt%, 1 to 99 wt%, 1 to 90 wt%, 1 to 80 wt%, 1 to 70 wt%, 1 to 60 wt%, 1 to 50 wt%, and the like 1 to 40 weight percent, 1 to 30 weight percent, 1 to 20 weight percent, 1 to 10 weight percent, 5 to 99 weight percent, 5 to 90 weight percent, 5 to 80 weight percent, 5 to 70 weight percent, 5 to 60 weight percent, 5 to 50 weight percent, 5 to 40 weight percent, 5 to 30 weight percent, 5 to 20 weight percent, 5 to 10 weight percent, 10 to 99 weight percent, 10 to 90 weight percent, 10 to 80 weight percent, 10 to 70 weight percent, 10 to 60 weight percent, 10 to 50 weight percent, 10 to 40 weight percent, 10 to 30 weight percent, 10 to 20 weight percent, 20 to 99 weight percent, 20 to 90 weight percent, 20 to 80 weight percent, 20 to 70 weight percent, 20 to 60 weight percent, 20 to 50 weight percent, 20 to 40 weight percent, 20 to 30 weight percent, 30 to 99 wt%, 30 to 90 wt%, 30 to 80 wt%, 30 to 70 wt%, 30 to 60 wt%, 30 to 50 wt%, 30 to 40 wt%, 40 to 99 wt%, 40 to 90 wt%, 40 to 80 wt%, 40 to 70 wt%, 40 to 60 wt%, 40 to 50 wt%, 50 to 99 wt%, 50 to 90 wt%, 50 to 80 wt%, 50 to 70 wt%, 50 to 60 wt%, 60 to 99 wt%, 60 to 90 wt%, 60 to 80 wt%, or 60 to 70 wt%.
In addition to containing the active ingredient, the cosmetic compositions provided herein may further contain ingredients commonly used in cosmetic compositions, for example, may further contain one or more selected from the group consisting of common adjuvants (such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and flavoring agents) and carriers. As the carrier, one or more selected from purified water, monohydric alcohol (ethanol or propanol), polyhydric alcohol (glycerin, 1, 3-butylene glycol or propylene glycol), higher fatty acid (palmitic acid or threonic acid), fat and oil (wheat germ oil, camellia oil, jojoba oil, olive oil, squalene, sunflower oil, macadamia nut (macadamia nut) oil, avocado oil or fatty glyceride) and the like can be used, but are not limited thereto. In addition, if necessary, one or more selected from surfactants, moisturizers, preservatives, antioxidants, and the like may be added.
The formulation of the cosmetic composition may be in all forms applied to the hair, for example, may be in one or more selected from hair products such as solutions, sprays, emulsions, gels, creams, essences, masks, ampoules (samples), gels, shampoos, soaps, oils, etc.
The content of the active ingredient (active compound (sulforaphane, indole-3-methanol, beta-sitosterol, or a combination thereof) and/or extract (solid)) contained in the cosmetic composition may be appropriately determined depending on the form of formulation, desired effect, etc., for example, the total weight of the cosmetic composition may be 0.0001 to 99 wt%, 0.0001 to 90 wt%, 0.0001 to 80 wt%, 0.0001 to 70 wt%, 0.0001 to 60 wt%, 0.0001 to 50 wt%, 0.0001 to 40 wt%, 0.0001 to 30 wt%, 0.0001 to 20 wt%, 0.0001 to 10 wt%, 0.01 to 99 wt%, 0.01 to 90 wt%, 0.01 to 80 wt%, 0.01 to 70 wt%, 0.01 to 60 wt%, 0.01 to 50 wt%, 0.01 to 40 wt%, 0.01 to 30 wt%, 0.01 to 20 wt%, 0.01 to 10 wt%, 1 to 99 wt%, 1 to 90 wt%, 1 to 80 wt%, 1 to 70 wt%, 1 to 60 wt%: 1 to 50 wt%, 1 to 40 wt%, 1 to 30 wt%, 1 to 20 wt%, 1 to 10 wt%, 5 to 99 wt%, 5 to 90 wt%, 5 to 80 wt%, 5 to 70 wt%, 5 to 60 wt%, 5 to 50 wt%, 5 to 40 wt%, 5 to 30 wt%, 5 to 20 wt%, 5 to 10 wt%, 10 to 99 wt%, 10 to 90 wt%, 10 to 80 wt%, 10 to 70 wt%, 10 to 60 wt%, 10 to 50 wt%, 10 to 40 wt%, 10 to 30 wt%, 10 to 20 wt%, 20 to 99 wt%, 20 to 90 wt%, 20 to 80 wt%, 20 to 70 wt%, 20 to 60 wt%, 20 to 50 wt%, 20 to 40 wt% 20 to 30 wt%, 30 to 99 wt%, 30 to 90 wt%, 30 to 80 wt%, 30 to 70 wt%, 30 to 60 wt%, 30 to 50 wt%, 30 to 40 wt%, 40 to 99 wt%, 40 to 90 wt%, 40 to 80 wt%, 40 to 70 wt%, 40 to 60 wt%, 40 to 50 wt%, 50 to 99 wt%, 50 to 90 wt%, 50 to 80 wt%, 50 to 70 wt%, 50 to 60 wt%, 60 to 99 wt%, 60 to 90 wt%, 60 to 80 wt%, or 60 to 70 wt%.
The health functional food is a food manufactured using raw materials or components (hereinafter, "functional raw materials") that are easily lacking in daily diet or have useful functions to the human body, and refers to any food that helps to maintain health or prevent and/or improve certain diseases or symptoms, and there is no particular limitation on the form of the final product. For example, the health functional food may be selected from various foods, beverages, food additives, etc., but is not limited thereto.
The content of the active ingredient (active compound (sulforaphane, indole-3-methanol, beta-sitosterol, or a combination thereof) and/or extract (solid matter)) contained in the food composition may be appropriately determined depending on the form of formulation, desired effect, etc., for example, the total weight of the food composition may be 0.0001 to 99 wt%, 0.0001 to 90 wt%, 0.0001 to 80 wt%, 0.0001 to 70 wt%, 0.0001 to 60 wt%, 0.0001 to 50 wt%, 0.0001 to 40 wt%, 0.0001 to 30 wt%, 0.0001 to 20 wt%, 0.0001 to 10 wt%, 0.01 to 99 wt%, 0.01 to 90 wt%, 0.01 to 80 wt%, 0.01 to 70 wt%, 0.01 to 60 wt%, 0.01 to 50 wt%, 0.01 to 40 wt%, 0.01 to 30 wt%, 0.01 to 20 wt%, 0.01 to 10 wt%, 1 to 99 wt%, 1 to 90 wt%, 1 to 80 wt%, 1 to 70 wt%, 1 to 60 wt%, 1 to 50 wt% >, and 1 to 40 weight percent, 1 to 30 weight percent, 1 to 20 weight percent, 1 to 10 weight percent, 5 to 99 weight percent, 5 to 90 weight percent, 5 to 80 weight percent, 5 to 70 weight percent, 5 to 60 weight percent, 5 to 50 weight percent, 5 to 40 weight percent, 5 to 30 weight percent, 5 to 20 weight percent, 5 to 10 weight percent, 10 to 99 weight percent, 10 to 90 weight percent, 10 to 80 weight percent, 10 to 70 weight percent, 10 to 60 weight percent, 10 to 50 weight percent, 10 to 40 weight percent, 10 to 30 weight percent, 10 to 20 weight percent, 20 to 99 weight percent, 20 to 90 weight percent, 20 to 80 weight percent, 20 to 70 weight percent, 20 to 60 weight percent, 20 to 50 weight percent, 20 to 40 weight percent, 20 to 30 weight percent, 30 to 99 wt%, 30 to 90 wt%, 30 to 80 wt%, 30 to 70 wt%, 30 to 60 wt%, 30 to 50 wt%, 30 to 40 wt%, 40 to 99 wt%, 40 to 90 wt%, 40 to 80 wt%, 40 to 70 wt%, 40 to 60 wt%, 40 to 50 wt%, 50 to 99 wt%, 50 to 90 wt%, 50 to 80 wt%, 50 to 70 wt%, 50 to 60 wt%, 60 to 99 wt%, 60 to 90 wt%, 60 to 80 wt%, or 60 to 70 wt%.
The health functional food may further contain one or more additives selected from the group consisting of: various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic or natural flavoring agents, coloring agents, modifiers (cheese, chocolate, etc.), pectic acid or salts thereof, alginic acid or salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonating agents for carbonated beverages, etc. The ratio of the additive is generally selected in the range of about 0.00001 to about 20 parts by weight based on 100 parts by weight of the total health functional food, but is not limited thereto.
The compositions and/or active ingredients (active compounds and/or extracts) provided herein may be administered in a pharmaceutically effective dose. As used herein, a "pharmaceutically effective dose" or "effective dose" may refer to a dose that contributes to a desired effect (promoting hair growth and/or inhibiting hair loss), or exhibits a beneficial effect on that effect, or achieves that effect. Pharmaceutically effective dosages may be prescribed in various manners depending on factors such as a formulation method, age, body weight, sex, symptom level, administration time, administration interval, excretion rate, response sensitivity, and the like of the subject to be administered. The effective dose may vary depending on the age, weight, sex, administration form, health condition, symptom level, etc. of the subject, and the effective dose may be administered 1 time a day to several times at fixed small intervals at the discretion of a doctor or pharmacist.
For example, the single or daily dose of the composition may be in the following range, based on the weight of the active ingredient (active compound (sulforaphane, indole-3-methanol, β -sitosterol, or combinations thereof) and/or extract (solid)). 0.0001 to 10000mg/kg, specifically, 0.001 to 1000mg/kg, 0.001 to 500mg/kg, 0.001 to 300mg/kg, 0.001 to 250mg/kg, 0.01 to 1000mg/kg, 0.01 to 500mg/kg, 0.01 to 300mg/kg, 0.01 to 250mg/kg, 0.1 to 1000mg/kg, 0.1 to 500mg/kg, 0.1 to 300mg/kg, 0.1 to 250mg/kg, 1 to 1000mg/kg, 1 to 500mg/kg, 1 to 300mg/kg, 1 to 250mg/kg, 10 to 1000mg/kg, 10 to 500mg/kg, 10 to 300mg/kg, or 10 to 250mg/kg, but not limited thereto. Single or daily doses may be formulated as unit dosage forms as a single formulation, or by appropriate division into several parts, or by internalization in a multi-dose container. The dose is an example of an average case, and depending on individual differences, the dose may be higher or lower.
Advantageous effects
By containing broccoli (broccoli) extract, avocado (avocado) extract, and mixtures thereof or effective compounds derived from these natural extracts, the present specification provides a composition for promoting hair growth or inhibiting hair loss which has excellent biosafety and has excellent hair growth-promoting or hair loss-inhibiting effects.
Drawings
Fig. 1a is a graph showing testosterone concentration in serum of rats dosed with broccoli extract, avocado extract, or a mixture thereof, according to one embodiment.
Fig. 1b is a graph showing the concentration of Dihydrotestosterone (DHT) in serum of rats dosed with broccoli extract, avocado extract, or a mixture thereof according to one embodiment.
Fig. 2 is a photograph of a stained skin tissue of a rat administered with broccoli extract, avocado extract, or a mixture thereof, according to one embodiment.
Fig. 3 is a graph showing hair follicle length in the skin of a rat dosed with broccoli extract, avocado extract, or a mixture thereof, according to one embodiment.
Fig. 4a is a graph showing testosterone concentrations in serum of rats dosed with each or a combination of sulforaphane, indole-3-methanol, and β -sitosterol.
FIG. 4b is a graph showing the concentration of dihydrotestosterone in the serum of rats dosed with each or a combination of sulforaphane, indole-3-methanol and beta-sitosterol.
FIG. 5 is a graph showing hair follicle length in the skin of rats administered with each of sulforaphane, indole-3-methanol, and beta-sitosterol, or a combination thereof.
Examples
Hereinafter, the present invention will be described in more detail by the following examples. However, they are only intended to illustrate the present invention, and the scope of the present invention is not limited by these examples.
Example 1: preparation of the extract
1.1. Preparation of broccoli extract
The broccoli (broccoli) is washed with clear water and then dried sufficiently. To 100g of a powder obtained by pulverizing and drying broccoli, 10 times the volume (500 ml) of water or ethanol (10 to 100% (v/v) ethanol (ethanol)) was added, followed by extraction at 70℃for 3 hours or more, and after 3 times, the filtrate filtered through a 1 μm (micrometer) filter was concentrated under heating until it reached 10% (w/w) of the original weight. The obtained concentrated solution was continuously concentrated, completely dried, and then powdered to prepare a broccoli extract powder. Among them, broccoli water extract powder (hereinafter, referred to as BE) using water as an extraction solvent was used for the subsequent test.
1.2. Preparation of avocado extract
Avocado (avocado) was sterilized in chlorinated water for 15 minutes. The avocado flesh was separated and homogenized after addition of 1% (v/v) citric acid solution. The homogenized solution was lyophilized and powdered and stored frozen for 1 week prior to extraction. 100g of the prepared lyophilized powder was dissolved in 500mL of petroleum ether (CAS No. 101316-46-5) and extracted with a Soxlet extractor to obtain an oily extract. The extracted oil was pulverized to prepare avocado extract powder (hereinafter, referred to as AE).
Example 2: confirmation of the Male hormone-lowering Effect of the extract of broccoli and/or avocado
The testosterone and dihydrotestosterone (hereinafter, referred to as DHT) lowering effect of the broccoli extract and/or avocado extract prepared in example 1 was confirmed by collecting plasma of male wistar albino rats and using testosterone and DHT ELISA kit.
Example 2-1 raising of laboratory animals
Male wistar albino rats weighing 180-240 g and 2-3 months old were bred under conditions of constant light and shade cycle, temperature 22-25deg.C and relative humidity 60% in the light time from 8 a.m. to 8 a.m.. For the feed of experimental animals, a general pellet dry feed was used, and feed and water were allowed to be taken at any time.
Example 2-2 measurement of testosterone and DHT concentrations after extract administration
0.2ml of testosterone prepared by injecting 100mg of testosterone commercially available per day into the experimental animals raised in example 2-1) A solution of testosterone in 100ml peanut oil was diluted for a total of 21 days.
In addition, the broccoli extract and/or the avocado extract prepared in example 1 were orally administered daily for 21 days such that the dose was 100mg of the extract per 1kg of the body weight of each experimental animal (67 mg/kg of the broccoli extract+33 mg/kg of the avocado extract in the case of the co-administration group).
Groups of experimental animals treated with testosterone solution, broccoli extract, avocado extract, or a mixture of broccoli extract and avocado extract are listed in table 1 below.
TABLE 1
Testosterone solution and broccoli extract, avocado extract or broccoli extract and avocado extract were administered for 21 days, and on the next day, plasma from rats was extracted and testosterone and DHT concentrations were measured using testosterone and DHT ELISA kit (Arigo Biolaboratories company, taiwan, china) according to manufacturer's instructions prior to collecting skin samples.
The results of testosterone and DHT measurements in plasma from the normal, negative control and experimental groups are shown in fig. 1a and 1b, respectively. As shown in fig. 1a and 1b, the concentrations of testosterone and DHT in plasma were greatly increased compared to the normal group in the case of the negative control group administered with testosterone solution alone. On the other hand, in the case of the experimental group (groups 3, 4, 5) administered with the broccoli extract and/or the avocado extract, the concentrations of testosterone and DHT in the plasma were reduced, among them, in particular, in the case of the experimental group (group 5) administered with the mixture of the broccoli extract and the avocado extract, the concentrations of testosterone and DHT in the plasma were significantly reduced to be at the same level as in the normal group (group 1).
Example 3: confirmation of hair follicle effect by broccoli extract or avocado extract
Example 3-1 shape change of hair follicle (dyed photograph)
The shape change of hair follicles was studied by staining skin tissues of experimental animals. Test animals prepared in example 2-1 were dosed with testosterone solution and broccoli extract, avocado extract or a mixture thereof for 21 days, and on the next day, a dehairing drug was used (dehairing Mao Gao) The skin of the dorsal area of the experimental animal was then removed by incision and fixed with 10% (v/v) formalin. After 24 hours, skin samples were soaked in paraffin and cut to a uniform thickness of 10 μm and hair follicles were stained with hematoxylin and eosin. Slides were observed under a microscope and images were recorded using standard software (Leica, germany).
The photographs of the skin tissues of the normal, negative control and experimental groups observed are shown in fig. 2. As shown in fig. 2, in the case of the negative control group administered with testosterone solution only, many hair follicles were found to be in telogen phase. On the other hand, in the case of the experimental group (groups 3, 4, 5) to which the broccoli extract and/or the avocado extract were administered, the hair follicle length was increased, and the hair follicle length was found to be similar to that of the normal group (group 1).
Example 3-2 measurement of follicle Density
The hair follicle density in the skin of the normal, negative control and experimental groups was measured and is shown in table 2 below. The hair follicle density is determined by counting the unit area (1 mm 2 ) Is measured by the number of hair follicles. The results obtained are shown in table 2.
TABLE 2
Hair follicle density Rate of change compared to normal group
Normal group (group 1) 1.31±0.021 -
Negative control group (group 2) 0.91±0.069 -30.53％
Experiment group (group 3) 2.40±0.053* +83.21％
Experiment group (group 4) 2.09±0.032* +59.54％
Experiment group (group 5) 2.61±0.057* +99.24％
(in table 2, the values are mean ± SEM, n=15, p <0.05 compared to the negative control group
The rate of change compared to the normal group described in table 2 is calculated according to the following equation 1.
(equation 1)
Rate of change compared to the normal group= (hair follicle density of negative control group or experimental group-hair follicle density of normal group)/hair follicle density of normal group
As shown in table 2 above, the hair follicle density was reduced by about 31% in the case of the negative control group administered with testosterone solution alone, as compared to the normal group. On the other hand, in the case of the experimental group (groups 3, 4, 5) dosed with broccoli extract and/or avocado extract, they exhibited excellent effects of increasing the hair follicle density by about 83% (broccoli extract), about 60% (avocado extract), or about 99% (mixture of broccoli extract and avocado extract), respectively, compared to the normal group.
Examples 3-3 comparison of follicular condition
The ratio of the anagen phase to the telogen phase in the skin of the normal group, the negative control group and the experimental group was calculated and is shown in table 3 below.
TABLE 3
Growing period Rest period Growth phase/rest phase Rate of change compared to normal group
Normal group (group 1) 66.1 33.9 1.949853 -
Negative control group (group 2) 14.6 85.4 0.17096 -91.23％
Experiment group (group 3) 60.4 39.6 1.525253* -21.78％
Experiment group (group 4) 51.2 48.8 1.04918* -46.19％
Experiment group (group 5) 65.6 34.4 1.906977* -2.20％
P <0.05 compared to negative control group
The rate of change compared to the normal group in table 3 is calculated according to the following equation 2.
(equation 2)
Rate of change compared to the normal group= (growth period/rest period ratio of negative control group or experimental group-growth period/rest period ratio of normal group)/growth period/rest period ratio of normal group
As shown in table 3, in the case of the negative control group administered with testosterone solution alone, hair follicles in anagen phase decreased and hair follicles in telogen phase increased compared to the normal group, and thus the ratio of anagen phase/telogen phase was decreased by about 91%. On the other hand, in the case of all experimental groups (groups 3, 4, 5) dosed with broccoli extract and/or avocado extract, hair follicles in anagen phase were increased and hair follicles in telogen phase were decreased, compared with the negative control group. Among them, in particular, in the case of the experimental group (group 5) administered with a mixture of broccoli extract and avocado extract, hair follicles in anagen phase were greatly increased and hair follicles in telogen phase were significantly decreased, compared with the negative control group, and it was shown that the ratio of anagen/telogen phase was at a level similar to that of the normal group.
Examples 3-4 comparison of follicle Length
Hair follicle lengths in the skin of the normal, negative control, and experimental groups were measured and are shown in fig. 3. The stained hair follicle length photographed in example 3-1 was measured for hair follicle length using standard software (Leica, germany), and the results are shown in fig. 3.
As shown in fig. 3, a substantial decrease in hair follicle length was found in the case of the negative control group administered with testosterone solution alone, as compared to the normal group. On the other hand, in the case of the experimental group (groups 3, 4, 5) dosed with broccoli extract and/or avocado extract, the hair follicle length was increased compared to the negative control group. Among them, in particular, in the case of the experimental group administered with the mixture of the broccoli extract and the avocado extract, the hair follicle length was significantly increased as compared with the negative control group, and it exhibited excellent effects of the hair follicle length increase as compared with the normal group.
Examples 3 to 5 confirmation of the Effect of the extraction solvent from broccoli
An aqueous extract of broccoli was obtained according to example 1-1 by extracting broccoli with water. As a comparative group, broccoli extract was obtained in the same manner as in example 1-1, except that the extraction solvent was extracted with various concentrations (10%, 30%, 50%, 70%, 100%) of ethanol aqueous solution.
The content of Sulforaphane (SFN) in the broccoli water extract (DW) and the comparative group (EtOH; ethanol extracts extracted with different concentrations of ethanol) was measured and the results are shown in Table 4 below.
TABLE 4
Extraction solvent SFN content (μg/ml)
100％EtOH ND
70％EtOH ND
50％EtOH ND
30％EtOH 1.7
10％EtOH 5.2
DW 61.41
(ND: undetected)
As shown in table 4 above, it was confirmed that all extracts obtained by extracting broccoli with water or ethanol aqueous solutions of various concentrations (30% and 10%) contained sulforaphane. Among them, it was confirmed that the extract obtained by extracting broccoli with water was particularly high in sulforaphane content.
The water extract of broccoli and the ethanol extract of broccoli were administered to the experimental animals prepared in example 2-1 by the same method as in example 3-2, and the hair follicle density in the skin of the experimental animals was measured, and the results are shown in table 5 below. The normal control group (control; normal) was a group to which neither testosterone nor extract was administered, and the negative control group (testosterone; T) was a group to which testosterone alone was administered.
TABLE 5
Extraction solvent Hair follicle density
Control 1.25±0.39
Testosterone (Testosterone) 0.98±0.23
T+70％EtOH 0.99±0.48
T+30％EtOH 1.0±0.65
T+10％EtOH 1.14±0.73
T+DW 1.93±0.102
Hair follicle density is shown as mean ± mean standard deviation. The rate of change from the normal group was calculated by the same method as in equation 1 above.
As shown in table 5 above, it was confirmed that when the extract obtained by extracting broccoli with water or ethanol aqueous solutions of various concentrations was administered, the hair follicle density decreased by testosterone was increased again, as compared with the negative control group administered only with testosterone solution. In particular, it was confirmed that when the extract was obtained by extracting broccoli with water, the hair follicle density was increased more significantly.
Example 4: confirmation of androgen-lowering Effect of Compounds
0.2ml of testosterone prepared by injecting 100mg of testosterone commercially available per day into the experimental animals raised in example 2-1) A solution of testosterone in 100ml peanut oil was diluted for a total of 21 days and according to Table 6 below, 10mg/kg of sulforaphane, indole-3-methanol, beta-sitosterol or a mixture of these compounds was orally administered daily for 21 days.
The experimental animal groups treated with sulforaphane, indole-3-methanol, beta-sitosterol or a mixture of these compounds are listed in table 6 below.
TABLE 6
The administration of sulforaphane, indole-3-methanol, beta-sitosterol or mixtures thereof was carried out for 21 days, and on the next day, plasma from rats was extracted and testosterone and DHT concentrations were measured using testosterone and DHT ELISA kit (Arigo Biolaboratories company, taiwan) (see example 2-2).
The results of the testosterone and DHT concentration measurements in plasma from the normal (control), negative control and experimental groups are shown in fig. 4a (testosterone) and fig. 4b (DHT), respectively. As shown in fig. 4a and 4b, the concentrations of testosterone and DHT in plasma were greatly increased in the case of the negative control group administered with testosterone solution alone, compared to the normal group. On the other hand, it can be seen that in the case of the experimental group (group C, D, E, F) administered with sulforaphane, indole-3-methanol and/or β -sitosterol, the concentrations of testosterone and DHT in the plasma were significantly reduced. Among them, in particular, in the case of the experimental group (group F) to which a mixture of glucoraphanin, indole-3-methanol and β -phytosterol was administered, the concentrations of testosterone and DHT in plasma were greatly reduced to be at the same level as the normal group (group a).
Example 5: confirmation of follicular effect by Compounds
Example 5-1 measurement of follicle Density
Referring to example 3-2, the hair follicle density in the skin of the normal group, the negative control group and the experimental group was measured and is shown in table 7 below.
TABLE 7
Hair follicle density Rate of change compared to normal group
Normal group (group A) 1.31±0.021 -
Negative control group (group B) 0.91±0.069 -30.53％
Experiment group (group C) 1.93±0.102* +47.33％
Experiment group (group D) 1.59±0.74* +21.37％
Experiment group (group E) 2.195±0.093* +67.56％
Experiment group (group F) 2.41±0.064* +83.97％
Values are mean ± SEM, n=15, p <0.05 compared to negative control group
Hair follicle density is shown as mean ± mean standard deviation. The rate of change from the normal group was calculated by the same method as in equation 1 above.
As shown in table 7 above, the hair follicle density was reduced by about 31% in the case of the negative control group administered with testosterone solution alone, compared to the normal group. On the other hand, the experimental group (group C, D, E, F) administered with sulforaphane, indole-3-methanol, β -sitosterol or a mixture thereof exhibited excellent effects of increasing the hair follicle density by about 47% (sulforaphane), about 21% (indole-3-methanol), about 68% (β -sitosterol) or about 84% (a mixture of sulforaphane, indole-3-methanol and β -sitosterol), respectively, compared to the normal group.
Example 5-2 comparison of follicular conditions
Referring to examples 3-3, the ratio of the anagen phase to the telogen phase of hair follicles in the skin of the normal group, the negative control group and the experimental group was calculated and is shown in table 8 below.
TABLE 8
Growing period Rest period Growth phase/rest phase Rate of change compared to normal group
Normal group (group A) 66.1 33.9 1.94 -
Negative control group (group B) 14.6 85.4 0.17 -91.23％
Experiment group (group C) 50.8 49.2 1.03* -47.05％
Experiment group (group D) 46.8 53.2 0.87* -54.88％
Experiment group (group E) 50.2 49.8 1.00* -48.30％
Experiment group (group F) 58.4 41.6 1.40* -28.00％
* Refers to p <0.05 compared to the negative control group
The rate of change compared to the normal group is calculated by the same method as equation 2.
As shown in table 8 above, in the case of the negative control group administered with testosterone solution alone, hair follicles in anagen phase decreased and hair follicles in telogen phase increased compared to the normal group, so that the ratio of anagen phase/telogen phase was reduced by about 91%. On the other hand, in the case of the experimental group (group C, D, E, F) to which sulforaphane, indole-3-methanol, β -sitosterol, or a mixture thereof was administered, hair follicles in the anagen phase were increased and hair follicles in the telogen phase were decreased, compared with the negative control group. Among them, in particular, it was confirmed that in the case of the experimental group (group F) administered with a mixture of testosterone with sulforaphane, indole-3-methanol and β -phytosterol, hair follicles in anagen phase were greatly increased and hair follicles in telogen phase were significantly decreased as compared with the negative control group.
Examples 5-3 comparison of follicle lengths
Referring to examples 3-4, hair follicle lengths in the skin of the normal, negative control, and experimental groups were measured and are shown in fig. 5. As shown in fig. 5, the hair follicle length was greatly reduced in the case of the negative control group administered with testosterone alone solution, compared to the normal group (control). On the other hand, in the case of the experimental group (group C, D, E, F) to which sulforaphane, indole-3-methanol, β -sitosterol, or a mixture thereof was administered, the hair follicle length was increased as compared with the negative control group. Among them, in particular, it was confirmed that the hair follicle length was greatly increased in the case of the experimental group (group F) to which a mixture of testosterone with sulforaphane, indole-3-methanol and β -sitosterol was administered, as compared with the negative control group.
Examples 5 to 4 confirmation of effects according to the mixing ratio of the Compounds
To the experimental animals raised in example 2-1, a mixture of sulforaphane, indole-3-methanol and β -sitosterol was mixed by the same method as example 4 in various mixing ratios as in the following table 9 at a dose of 10mg/kg per day for a total of 21 days. The normal control group (control; normal) was a group to which neither testosterone nor extract was administered, and the negative control group (testosterone; T) was a group to which testosterone alone was administered.
Referring to example 3-2, the hair follicle density in the skin of the normal group, the negative control group and the experimental group was measured and is shown in table 9 below.
TABLE 9
( SFN: sulforaphane; I3C: indole-3-methanol; s: beta-sitosterol )
Hair follicle density is shown as mean ± mean standard deviation. The rate of change from the normal group was calculated by the same method as in equation 1 above.
As shown in table 9 above, the hair follicle density was reduced by about 22% in the case of the negative control group dosed with testosterone solution alone, compared to the normal group. However, it was confirmed that the hair follicle density was significantly increased in all cases where the mixture of sulforaphane, indole-3-methanol and β -sitosterol was administered in various mixing ratios, as compared with the negative control group.
Examples 5 to 5 confirmation of the Effect of the administration of Compounds
To the experimental animals raised in example 2-1, a mixture of raphanin, indole-3-methanol and β -sitosterol was mixed in the same manner as in example 4 at a ratio of 8:1:1 by oral administration daily at a dose of 10mg/kg (SFN 8 mg/kg+I3C1 mg/kg+S1 mg/kg) for a total of 21 days. As a comparative group, the mixture was administered by the same method, but the administration mode was the intraperitoneal administration (IP) method. The normal control group (control; normal) was a group to which neither testosterone nor extract was administered, and the negative control group (testosterone; T) was a group to which testosterone alone was administered.
Referring to example 3-2, the hair follicle density in the skin of the normal group, the negative control group and the experimental group was measured and is shown in the following table 10.
TABLE 10
Administration mode Hair follicle density Rate of change compared to normal group
Control 1.25±0.39 -
Testosterone (Testosterone) 0.98±0.23 -21.6％
T+ oral administration 2.41±0.064 92.8％
T+IP injection 1.37±0.051 9.6％
As shown in table 10 above, the hair follicle density was reduced by about 22% in the case of the negative control group administered with testosterone solution alone, compared to the normal group. It was confirmed that the hair follicle density was increased in all experimental groups to which the mixture was administered intraperitoneally (IP injection) or orally (oral administration) compared to the negative control group, and in particular, the hair follicle density was more significantly increased in the experimental group to which oral administration was performed.
From the above description, those skilled in the art to which the invention pertains will appreciate that the invention may be practiced in other embodiments without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are illustrative in all respects, rather than restrictive. The scope of the invention should be construed as being derived from the meaning and scope of the claims and equivalents thereof below, and not all changes or modifications derived from the detailed description.

Claims (9)

1. A composition for promoting hair growth or preventing, alleviating or treating alopecia, comprising one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, beta-sitosterol (beta-sitosterol) and salts thereof as an active ingredient.
2. A composition for promoting hair growth or preventing, relieving or treating alopecia comprises broccoli (broccoli) extract, avocado (avocado) extract or their mixture as active ingredient.
3. The composition for promoting hair growth according to claim 2, wherein the broccoli extract is extracted with one or more extraction solvents selected from water and linear or branched alcohols having 1 to 4 carbon atoms; and the avocado extract is extracted with petroleum ether solution.
4. A cosmetic composition for promoting hair growth or preventing, alleviating or treating alopecia comprises one or more substances selected from the group consisting of sulforaphane, indole-3-methanol, beta-sitosterol (beta-sitosterol) and salts thereof as active ingredients.
5. A cosmetic composition for promoting hair growth or preventing, relieving or treating alopecia comprises broccoli (broccoli) extract, avocado (avocado) extract or their mixture as active ingredient.
6. The cosmetic composition for promoting hair growth according to claim 5, wherein the broccoli extract is extracted with one or more extraction solvents selected from water and a linear or branched alcohol having 1 to 4 carbon atoms, and the avocado extract is extracted with a petroleum ether solution.
7. A health functional food for promoting hair growth or preventing, relieving or treating alopecia comprises one or more substances selected from sulforaphane, indole-3-methanol, beta-sitosterol (beta-sitosterol) and their salts as active ingredients.
8. A health functional food for promoting hair growth or preventing, improving or treating alopecia comprises broccoli (broccoli) extract, avocado (avocado) extract or their mixture.
9. The health functional food according to claim 8, wherein the broccoli extract is extracted with one or more extraction solvents selected from water and a linear or branched alcohol having 1 to 4 carbon atoms, and the avocado extract is extracted with a petroleum ether solution.
CN202180079360.3A 2020-10-07 2021-10-06 Composition for promoting hair growth and/or inhibiting hair loss Pending CN116600661A (en)

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