CN116593441A - Detection box and detection method for detecting novel coronavirus N protein based on SERS - Google Patents
Detection box and detection method for detecting novel coronavirus N protein based on SERS Download PDFInfo
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- CN116593441A CN116593441A CN202310198221.3A CN202310198221A CN116593441A CN 116593441 A CN116593441 A CN 116593441A CN 202310198221 A CN202310198221 A CN 202310198221A CN 116593441 A CN116593441 A CN 116593441A
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- 238000001514 detection method Methods 0.000 title claims abstract description 95
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 title claims abstract description 28
- 108700002099 Coronavirus Nucleocapsid Proteins Proteins 0.000 title claims abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 76
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000011324 bead Substances 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 11
- 238000001069 Raman spectroscopy Methods 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 10
- 238000001237 Raman spectrum Methods 0.000 claims description 8
- 239000010931 gold Substances 0.000 claims description 8
- 229910052737 gold Inorganic materials 0.000 claims description 8
- 239000002105 nanoparticle Substances 0.000 claims description 8
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 2
- 241000711573 Coronaviridae Species 0.000 abstract description 9
- 241000700605 Viruses Species 0.000 abstract description 3
- 238000011895 specific detection Methods 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 description 11
- 239000000427 antigen Substances 0.000 description 9
- 102000036639 antigens Human genes 0.000 description 9
- 108091007433 antigens Proteins 0.000 description 9
- 238000012360 testing method Methods 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 239000000523 sample Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000004557 single molecule detection Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 238000004454 trace mineral analysis Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/165—Coronaviridae, e.g. avian infectious bronchitis virus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention belongs to the technical field of virus detection, and discloses a detection box and a detection method for detecting novel coronavirus N protein based on SERS, wherein the detection box comprises a box main body, a cover plate and a base, the box main body is provided with a plurality of reagent storage bins and a reagent mixing bin, the reagent storage bins are mutually communicated through connecting channels and then are communicated with the reagent mixing bin, and the bottom of the reagent mixing bin is a conical groove; the cover plate is connected to the top of the reagent mixing bin in a bonding way, the cover plate is connected with a freely rotatable magnet, and the box main body is combined with the base in a bonding way. The detection box is simple in structure, convenient and practical, and can realize the specific detection of the novel coronavirus through the drainage of the injector and the control of the magnetic field, so that the operation difficulty of a method for detecting the novel coronavirus N protein based on SERS is reduced, and the detection efficiency is improved.
Description
Technical Field
The invention belongs to the technical field of virus detection, and particularly relates to a detection box and a detection method for detecting novel coronavirus N protein based on SERS.
Background
Aiming at the detection of novel coronaviruses, three detection methods of nucleic acid detection, antibody detection and antigen detection are mainly provided. Nucleic acid detection is a "gold standard" for clinical diagnosis in which a patient is sampled in the respiratory tract (i.e., nasal and pharyngeal swabs) and then subjected to fluorescent quantitative PCR to detect the RNA sequence of a novel coronavirus. Antibody detection is by drawing blood to detect whether there are antibodies in the blood, e.g., positive, meaning that the person has been infected with the novel coronavirus, or has been vaccinated. With the continued expansion of the worldwide vaccinated population, the role of antibody detection at the screening level has been insignificant. Antigen detection is directed to detection of the pathogen itself, and can detect whether a human body contains a virus in an early stage of the disease, thereby providing direct evidence of a viral infection. The speed of antigen detection can be faster, the operation is more convenient, and a detector can take the detection result in a short time. Therefore, antigen detection plays an early shunting and rapid management role in novel coronavirus epidemic prevention and control.
Surface enhanced Raman (Surface-EnhancedRamanScattering, SERS) is a method of initiating Raman enhanced scattering by plasma resonance (SurfacePlasmonResonance, SPR) interaction between probe molecules on or near the Surface of a metal nanostructure and the metal Surface, wherein the Raman signal generated by SERS is enhanced by 10 compared with ordinary Raman scattering 3 -10 14 Multiple times. Compared with other spectrum detection methods, SERS has three obvious advantages of high sensitivity, high selectivity and loose detection conditions, and can be widely applied to the fields of trace analysis, single-molecule detection, biomedical detection, surface adsorption, catalytic reaction and the like.
Currently, for antigen detection of novel coronaviruses, the main product on the market is an antigen test strip, but the antigen test strip has the defect of insufficient detection sensitivity. For other antigen detection methods, a new class of methods for SERS-based detection of coronavirus N proteins have been developed by the team, but the corresponding portable detection cartridges are lacking. Therefore, for a method of detecting novel coronavirus N protein based on SERS, a portable detection cartridge is urgently needed to provide a stable and rapid detection.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a detection box and a detection method for detecting novel coronavirus N protein based on SERS.
In order to achieve the above object, one of the technical solutions of the present invention is: the utility model provides a detect box based on SERS detects novel coronavirus N albumen, includes box main part, apron and base, the box main part is equipped with a plurality of reagent storage bins and a reagent and mixes the storehouse, communicates through connecting channel each other between a plurality of reagent storage bins again reagent mixes the storehouse, reagent mixes the storehouse bottom and is the toper recess, the apron bond is connected at reagent mixes the storehouse top, but apron bond a free rotation's magnet, the box main part combines through the bonding mode with the base.
In a preferred embodiment of the present invention, the bottom of the conical groove is a round flat bottom, and the round flat bottom is provided with a magnetic attraction detection point.
In a preferred embodiment of the invention, the side of the reagent mixing chamber remote from the reagent storage chamber is provided with a first syringe interface, which is connected to a syringe via a hose.
In a preferred embodiment of the invention, the reagent storage bin is used to store a pharyngeal swab collection solution, a magnetic bead solution, and a gold nanoparticle solution.
In a preferred embodiment of the invention, a second syringe interface is provided on top of the cover plate, the second syringe interface being connected to the syringe by a hose.
In a preferred embodiment of the invention, the magnetons are nested in a reagent mixing chamber.
In a preferred embodiment of the invention, the top of the reagent storage bin and the syringe interface are sealed by a sealing film.
In a preferred embodiment of the invention, the bottom of the box body is bonded to a base, which closes the bottom of the box body.
In order to achieve the above object, a second technical scheme of the present invention is as follows: the detection method of the detection box for detecting the novel coronavirus N protein based on SERS specifically comprises the following steps:
(1) All the reagents preloaded in a plurality of reagent storage bins of the detection box are introduced into a reagent mixing bin;
(2) Applying a rotating magnetic field to the bottom of the detection box to guide the magneton to rotate for incubation, stopping the rotation of the magnetic field, and enriching the magnetic beads in the conical grooves of the reagent mixing bin by utilizing the magnetic force of the magnetic field;
(3) Sucking all the reagents in the reagent mixing bin, leaving magnetic beads at the bottom of the bin, introducing cleaning liquid, re-rotating the magnetic field, stopping rotating the magnetic field after cleaning, and removing the magnetic field after the magnetic beads are enriched at the detection points;
(4) And the Raman detector emits laser irradiation detection points from the bottom of the detection box to collect signals, and integrates the obtained Raman spectrum signals to obtain signal intensity.
In a preferred embodiment of the present invention, the incubation time in step (2) is 3 to 5 minutes.
In a preferred embodiment of the present invention, the washing time in the step (3) is 20 to 30 seconds.
In a preferred embodiment of the present invention, the raman spectrum in step (4) has a wavelength in the range of 535 to 575nm.
Compared with the prior art, the invention has the beneficial effects that:
1. the detection box for detecting the novel coronavirus N protein based on SERS provided by the invention has a simple structure, is convenient and practical, can realize the specific detection of the novel coronavirus by virtue of the drainage of the injector and the control of the magnetic field, reduces the operation difficulty of a method for detecting the novel coronavirus N protein based on SERS, and improves the detection efficiency;
2. the detection box for detecting the novel coronavirus N protein based on SERS is preloaded with the reagent before use, does not need to carry other reagents to a detection site, and is convenient to carry.
3. The detection box can be directly carried to a detection site for extracting and detecting a novel coronavirus antigen sample, the detection method is simple and convenient, the detection result can be obtained within 5-10 minutes, and the detection efficiency is greatly improved;
drawings
FIG. 1 is an isometric view of a cartridge for detecting novel coronavirus N protein based on SERS of example 1;
FIG. 2 is a main line-of-sight frame diagram of a detection kit for detecting novel coronavirus N protein based on SERS of example 1;
FIG. 3 is a top line drawing of a detection cassette for detecting novel coronavirus N protein based on SERS of example 1;
FIG. 4 is a side view of a rack of detection cartridges for SERS-based detection of novel coronavirus N protein of example 1;
FIG. 5 is a top view of the detection cartridge of example 1 for detecting novel coronavirus N protein based on SERS;
FIG. 6 is a front view of a detection cassette for detecting novel coronavirus N protein based on SERS of example 1;
FIG. 7 is a graph of signal intensity versus histogram for the wavelength range 540-570nm for the Raman spectrum of example 2;
in the figure:
1-a box body; 2-cover plate; 3-a base; 4-a first syringe interface; 5-a second syringe interface; 6-connecting channels; 7-a conical groove; 8-magnons; 9-a throat swab collecting liquid storage bin; 10-a magnetic bead solution storage bin; 11-gold nanoparticle solution storage bin; 12-a reagent mixing bin; 13-a magnetic attraction detection point.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in more detail with reference to the accompanying drawings and specific embodiments, but the scope of the present invention is not limited to these embodiments. Like reference numerals refer to like elements throughout, and like reference numerals refer to like elements.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are based on directions or positional relationships shown in the drawings, are merely for convenience of description and simplification of description, and do not indicate or imply that the apparatus or element to be referred to must have a specific direction, be constructed and operated in the specific direction, and thus should not be construed as limiting the present invention; the terms "first," "second," "third," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "coupled," and the like are to be construed broadly, and may be fixedly coupled, detachably coupled, or integrally coupled, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
The utility model provides a detect box based on SERS detects novel coronavirus N albumen, includes box main part, apron and base, the box main part is equipped with a plurality of reagent storage bins and a reagent and mixes the storehouse, communicates through connecting channel each other between a plurality of reagent storage bins again reagent mixes the storehouse, reagent mixes the storehouse bottom and is the toper recess, the apron bond is connected at reagent mixes the storehouse top, but apron bond a free rotation's magnet, the box main part combines through the bonding mode with the base.
The bottom of the conical groove is a round flat bottom, and a magnetic attraction detection point is arranged on the round flat bottom.
The reagent mixing bin is provided with a first syringe interface on one side far away from the reagent storage bin, and the first syringe interface is connected with a syringe through a hose.
The reagent storage bin is used for storing throat swab collection liquid, magnetic bead solution and gold nanoparticle solution.
The top of the cover plate is provided with a second syringe interface, and the second syringe interface is connected with a syringe through a hose.
The magnetons are nested in the reagent mixing bin.
The top of the reagent storage bin is sealed by a sealing film, and the injector interface is sealed by the sealing film.
The bottom of the box main body is bonded with the base, and the base seals the bottom of the box main body.
The detection method of the detection box for detecting the novel coronavirus N protein based on SERS specifically comprises the following steps:
(1) All the reagents preloaded in a plurality of reagent storage bins of the detection box are introduced into a reagent mixing bin;
(2) Applying a rotating magnetic field to the bottom of the detection box to guide the magneton to rotate for incubation, stopping the rotation of the magnetic field, and enriching the magnetic beads in the conical grooves of the reagent mixing bin by utilizing the magnetic force of the magnetic field;
(3) Sucking all the reagents in the reagent mixing bin, leaving magnetic beads at the bottom of the bin, introducing cleaning liquid, re-rotating the magnetic field, stopping rotating the magnetic field after cleaning, and removing the magnetic field after the magnetic beads are enriched at the detection points;
(4) And the Raman detector emits laser irradiation detection points from the bottom of the detection box to collect signals, and integrates the obtained Raman spectrum signals to obtain signal intensity.
The incubation time in the step (2) is 3-5 minutes.
The cleaning time in the step (3) is 20-30 seconds.
The wavelength range of the Raman spectrum in the step (4) is 535-575nm.
Example 1
Referring to fig. 1 to 6, a detection cartridge for detecting novel coronavirus N protein based on SERS includes a cartridge body 1, a cover plate 2, and a base 3; wherein 3 reagent storage bins and 1 reagent mixing bin 12 are arranged in the box main body 1, the 3 reagent mixing bins are communicated with each other through a connecting channel 6 and then are communicated with the reagent mixing bin 12, the cover plate 2 is connected with a freely rotatable magnet 8, the magnet 8 can rotate under the control of a magnetic field, and the box main body 1, the cover plate 2 and the base 3 are combined in a bonding mode; the 3 reagent storage bins are respectively a throat swab collecting liquid storage bin 9 (pre-filled with throat swab collecting liquid), a magnetic bead solution storage bin 10 (pre-filled with magnetic bead solution) and a gold nanoparticle solution storage bin 11 (pre-filled with gold nanoparticle solution). The tops of the 3 reagent storage bins are sealed by sealing films before use, and the sealing films are taken down when the reagent storage bins are used; the side of the reagent mixing bin 12, which is far away from the reagent storage bin, is provided with a first syringe interface 4, the first syringe interface 4 is connected with a syringe through a hose, the first syringe interface 4 is sealed by a sealing film, and the first syringe interface 4 is connected with the syringe in use; the bottom of the reagent mixing bin 12 is provided with a conical groove 7, so that the magnetic beads can be conveniently enriched, the bottom of the conical groove 7 is provided with a round flat bottom, and the signals can be conveniently collected by laser irradiation; the round flat bottom is provided with a magnetic attraction detection point 13; the bottom of the box main body 1 is bonded with a base 3, and the base 3 seals the bottom of the box main body 1; the top of the reagent mixing bin 12 in the box main body 1 is bonded with a cover plate, the cover plate 2 is provided with a second syringe interface 5, and the second syringe interface 5 is sealed by a sealing film and is connected with a syringe in use.
Working principle: the invention provides a detection box for detecting novel coronavirus N protein based on SERS, which is characterized in that a throat swab sample collection liquid is preloaded in a throat swab collection liquid storage bin 9 of the detection box before use, a magnetic bead solution is preloaded in a magnetic bead solution storage bin 10, a gold nanoparticle solution is preloaded in a gold nanoparticle solution storage bin 11, and a sealing film is used for sealing. The cover plate 2 is put on the box body 1. When the kit is used, all sealing films are taken down firstly, the first injector interface 4 and the second injector interface 5 are connected with the first injector and the second injector respectively through hoses, after the collected throat swab rod is rinsed in the throat swab collecting liquid storage bin 9, all reagents are introduced into the reagent mixing bin 12 through the connecting channel 6 by the second injector through negative pressure, the bottom of the detection box is applied with a rotating magnetic field to guide the magnet 8 to rotate for incubation, the rotation of the magnetic field is stopped after incubation for 3-5 minutes, and the magnetic beads are fully enriched in the conical groove 7 of the reagent mixing bin by utilizing the magnetic force of the magnetic field. The first syringe draws all solution away by negative pressure, leaving the magnetic beads at the bottom of the cartridge. And (5) introducing cleaning liquid into the interface of the second injector, rotating the magnetic field again, stopping rotating the magnetic field after cleaning for 20-30 seconds, and removing the magnetic field after the magnetic beads are enriched at the magnetic attraction detection point 13. The Raman detector emits laser to irradiate the detection point from the bottom of the detection box to acquire signals, and the detection result can be obtained.
Example 2
The cartridge of example 1 was used for detection, and the specific detection method was as follows:
1 person is shared by the test group A, and the nucleic acid detection is negative; the test group B test persons have 1 person, and the nucleic acid detection is positive. Before the experiment starts, all sealing films on the detection box are torn, and the first syringe interface part 4 and the second syringe interface part 5 are respectively connected with the first syringe and the second syringe through hoses. And (3) collecting a throat swab of a subject at the beginning of an experiment, after rinsing the throat swab collecting liquid storage bin of the detection box, introducing all reagents into the reagent mixing bin by a second injector through negative pressure, applying a rotating magnetic field at the bottom of the box main body 1 to guide the magnet 8 to rotate for incubation, stopping the rotation of the magnetic field after incubation for 3-5 minutes, and fully enriching the magnetic beads in the conical groove 7 of the reagent mixing bin by utilizing the magnetic force of the magnetic field. The first syringe draws all solution away by negative pressure, leaving the magnetic beads at the bottom of the cartridge. And (5) introducing a cleaning solution into the interface of the second injector, re-rotating the magnetic field, stopping rotating the magnetic field after cleaning for 20-30 seconds, and removing the magnetic field after the magnetic beads are enriched at the detection point. The Raman detector emits laser irradiation detection points from the bottom of the detection box to collect signals. The collected signals are simply processed, and the signals in the wavelength range of 540-570nm of the obtained Raman spectrum are integrated, so that a signal intensity comparison histogram is obtained and is shown in figure 7. As can be seen from fig. 7: the Raman signal intensity detected by the negative patient is far lower than that of the positive patient, and the difference is obvious, so that the detection box provided by the invention has extremely high sensitivity, and whether the sampled person is infected with the novel coronavirus can be obviously distinguished.
The foregoing description is only illustrative of the preferred embodiments of the present invention, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements or changes may be made without departing from the spirit and principles of the present invention.
Claims (10)
1. The detection box for detecting novel coronavirus N protein based on SERS comprises a box main body, a cover plate and a base, and is characterized in that the box main body is provided with a plurality of reagent storage bins and a reagent mixing bin, the reagent storage bins are mutually communicated through a connecting channel and then are communicated with the reagent mixing bin, and the bottom of the reagent mixing bin is provided with a conical groove; the cover plate is connected to the top of the reagent mixing bin in a bonding way, the cover plate is connected with a freely rotatable magnet, and the box main body is combined with the base in a bonding way.
2. The cartridge of claim 1, wherein the magnet is nested within the reagent mixing chamber.
3. The cartridge of claim 1, wherein the bottom of the conical recess is a circular flat bottom provided with a magnetic detection point.
4. The cartridge of claim 1, wherein a first syringe interface is provided on a side of the reagent mixing cartridge remote from the reagent storage cartridge, the first syringe interface being connected to a syringe by a hose, a second syringe interface is provided on the top of the cover plate, and the second syringe interface being connected to the syringe by a hose.
5. The cartridge of claim 1, wherein the reagent storage compartment is for storing a pharyngeal swab collection solution, a magnetic bead solution, and a gold nanoparticle solution.
6. The cartridge of claim 1, wherein the bottom of the cartridge body is bonded to a base that closes the bottom of the cartridge body.
7. The method for detecting a cartridge according to any one of claims 1 to 6, comprising the steps of:
(1) All the reagents preloaded in a plurality of reagent storage bins of the detection box are introduced into a reagent mixing bin;
(2) Applying a rotating magnetic field to the bottom of the detection box to guide the magneton to rotate for incubation, stopping the rotation of the magnetic field, and enriching the magnetic beads in the conical grooves of the reagent mixing bin by utilizing the magnetic force of the magnetic field;
(3) Sucking all the reagents in the reagent mixing bin, leaving magnetic beads at the bottom of the bin, introducing cleaning liquid, re-rotating the magnetic field, stopping rotating the magnetic field after cleaning, and removing the magnetic field after the magnetic beads are enriched at the detection points;
(4) And the Raman detector emits laser irradiation detection points from the bottom of the detection box to collect signals, and integrates the obtained Raman spectrum signals to obtain signal intensity.
8. The method of claim 7, wherein the incubation time in step (2) is 3 to 5 minutes.
9. The method of claim 7, wherein the washing time in the step (3) is 20 to 30 seconds.
10. The method of claim 7, wherein the raman spectrum in step (4) has a wavelength in the range of 535 nm to 575nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310198221.3A CN116593441A (en) | 2023-03-03 | 2023-03-03 | Detection box and detection method for detecting novel coronavirus N protein based on SERS |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310198221.3A CN116593441A (en) | 2023-03-03 | 2023-03-03 | Detection box and detection method for detecting novel coronavirus N protein based on SERS |
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| Publication Number | Publication Date |
|---|---|
| CN116593441A true CN116593441A (en) | 2023-08-15 |
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| CN202310198221.3A Pending CN116593441A (en) | 2023-03-03 | 2023-03-03 | Detection box and detection method for detecting novel coronavirus N protein based on SERS |
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| Country | Link |
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| CN (1) | CN116593441A (en) |
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- 2023-03-03 CN CN202310198221.3A patent/CN116593441A/en active Pending
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